Carg09324-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg09324-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	acyl-protein thioesterase 2-like
Location	Cucurbita_argyrosperma_scaffold_018 : 610972 .. 614438 (+)
Sequence length	951

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGTGAAGGGACGCGAAGGCAAAGCTTTTACAAAAGAGACAGAAACTGGAAAAGCCGATATGCTTTTCGGGTCGTTAAACACTTCTATCCCAAATTTTTTGTAATTGGTAAATAAAAACCCTCAAATCCTCTTCACTTTCTGCTTCTGTTCTTCATATTTAAGTAAATTTTAAGCAATCTGTCCAGATCTTTCCTATGAATTCTCGCCGTCATCAATGCTTGCTTGAACATTCAACTTTTAGGTCTGTAATTTGATGGCTGTCTTATCATTCTTTATGTATTGTATTTTATACCCTTTTTTTTCTTTCTTTTTTTTTTTTTTTTTTTTTTTGCATAAAAAATGGTTGAAAATTTTGATTAGTTGCTATATTCTGGTGTTGCTGTAGTTGAAGTGCTTTAGCCTTGTGGGTTGTCCTGCGTTCACGGGCATAGATTCTTTGCCTCTCGCTCTCTTGCCCTCGTAATCCCTCTTTTTGTTGCTCCCTCTGTGGCATTATGAACTGAAGGGCTATGTCAGCTCAGAGCTCGTTTTCTGCATCGATGCTTTAATCTCTTGTGGATTCTTCTTTGCCCATAAAGATTTCTTGCCTTTTTCTTCCTTTTTTGAGATGCCTTTCACTTTTTTTAGTATTTGAGGACTTTGGAGGCTGTTTATGGGTTGTGATATCAGGAGTAGCCAATGGAAAATTCATGGGTTTTAGTTCCATTTCAGCCCTCCCAAGTGCATATAAATACAGTTGGTCAATTCTAAAACTCTTCATTGCAGGAACTAATTCCAACGCCCAAGTTGTTGCCTGTCTTGCTTCCAACATTTTTGTGGAAATACAATGAGCTATAACAGCTCTGCTATGGGTTCTGGTTTGTTCACTTTCTGCTCATTTTGTTTTATTGGTTTACTGGCCATCAAGCTAAACTAGCTAGCTTTCATTTTTGCTCCATTATGTCAGGTAGTAGAACAGGCAGGATGAGCTTTGAGTTTGGGAGGACCCATGTGGTCAGGCCTAAAGGGAAGCATCAGGCAACTATTGTTTGGTTACATGGCCTTGGTGATAAGGGGTCAAGGTACTAATGGCTTATCTTCTTTACGTTCTCCTTCCTGTTTAGCCAGCTTATTAGAGTTATTTTGCTAGTTTCTGGTTGACTTACTTCGATTGAGATTAAGAAGAAACTTTGAGGTACTACGTGAGATCCCACATCAGTAGGAGAGGGGAACGAAACATTGCTCATAAGGGTGTGGAAACCTCTCCCTACCAAAGGGGCTCAGAAGGGAAAGCCCAAAGAAGACAATATCTGCTAGTGGTGAGTTTGGGCTGTTACAAATGGTATCAAAGCCAGACACCGGGCGGTGTGCTAGTGAGGACGCTAGGTCTCCAAGGGGGTGGATTGTGAGATCCCACATCGGTTGGAGAGGAGAATGAAACATTACTTATAAGGGTGTGGAAACCTCTCTCTACCAGACGCGTTTTAAAACCTTAAAGACAAACCTGTAAAGGAAAGTCCAAAGAGGACAATAACGGCCCGCCGTGGGTTTGGGCTATTACATACTTGAGTATAAACACTCAATGAACTCTATGGCGCATTACATGTAACTTGCAAACTTTCCATCCTAATTAGTGGGTTATATCTGAGCAGAGGACTTTCTTTTTGTCTTCCATGCCAAAAGTAAGGGAAGAAGTGGCTGAGTGAGAAAATGTCAATGTCTTGCCCTATCTTCTTGTGTATGTTCATATATAGAAAGTTTTAAAAATTCTATATGCCTTTACTAGAGATTCTTATTCTGATTTCTTTTTAAGTGGGAAGGTAACGTCATGTTTTGTGTCCTTTATAATTAGAAAAAAAATCAAAATCAATATAAAATAATGCTCATTATCATATGCTAGCTGATATCAGACATTTACATCATTAAAGGAGGGCTGGTTTCTTCATTATTGGAAGCCTATCTGGACATCTAATTTATTATTTATTTACTTCTAAATTTTTTCAGTTGGTCTCAGCTCTTGGAAACTCTTCCTCTACCAAACGTAAGGCTACTTAACATAGTAACCTTGATTGTCATTAAGTTTTGGGATTTGTTTAATATGATCAATTAACATAATAATTACGAATTCTGTTGCAGATTAAGTGGATTTGTCCTACTGCTCCTACTCGTCCTGTGACGTTATTTGGTGGATTTCCTTGCACTGCATGTAAGACTATCCAAATTTCATAAGTAGATTTATTAAAATTATGAGCCAAATTCCTCGTATGAAAACGATATATTTATTCTTGGAATTAGTTTAAATTATGAGTTACTCTGCCGTTTCGTATCAGGGTTTGATGTGGGAGATATTTCAGAGGAGTCTCCTGATGATTTAGAAGGCTTGGATGCTGCAGCATCGCATGTTGCTAATCTTTTGTCTACAGAACCTGCTGATAGTATGTTGTTTCTTCTTGAAAAGCTTTTAAACTCCCCCCAGCTGACTTGCTGTTTGTCATTTAATCACTGAACTTAATCATTGGTGTGATACTCCTCTGGTTGGTCACGTATTAGATTACCCGTTATTGTTGTGTCAAGGACTCGAACCGGTAGTTCTCATTTAAAACTATTTCATATATACGGTTTTGAAACGAAGAACAATTATGAATTGTATGTAGGGTAGGTATAGATAGAGGTTTCATCTACCTTCTCATATCGTTTTTACAGATGATACTTGGTGTTTGGCATACAAATTTGTCATATTCTCACTCTTTTTCTTACTAACAGTTAAACTGGGTATTGGAGGCTTCAGTATGGGTGCTGCAACTGCTATTTACTCTGCGTCACGCCGTATTCTTGGGCAATATGGAAATGGAAACCTATACCCTATTAACCTTAGTGCAGTTGTTGGGCTTAGTGGCTGGCTTCCCTGTTCAAGGTTTGATTTTTGTTTGTGAATTATGAAGAAGCCGAAACACACAGTGCACGGTCGTGAGAAAGCCTACACGGGTGGCCTTTGTTCTTTATCAAAGCCTTTTTTTGTTGTAGATCTTTGAGGAACCAGGTCAGTGTGTCACACGAAGCTGCAAGGCGCGCTGCGTCCCTGCCCATTTTACTCTGTCATGGTTCAGGTACACTGCTAGTTTACGAGTTCAAGCATTAATGCACTCTTGTGAGAACTAATTTCTCTCAATTCCCTTAATCCTATTGAATTTTACTGAGCGGTTGGTTACAGGTGACGATGTCGTAGCTTACAAACACGGGGAGAAATCTGCACATACATTAAGTTCATCTGGATTTAGCAATCTAACATTTAAATCCTATAACGGGTATGTTCGTATCTCGACTTTCTAGTGCTTCTCGTTTTGTATGTCTGATAATAAGTAACAAATAAGATGGGATCTTGCAATGCAGGCTGGGCCATTACACCATTCCTGAGGAGATGGATGTTGTTTGTAATTGGCTGACAGTCGTTTTGGGGCTCGATGGCTTACGGTTGGTCGATCACTGA

mRNA sequence

GTGTGAAGGGACGCGAAGGCAAAGCTTTTACAAAAGAGACAGAAACTGGAAAAGCCGATATGCTTTTCGGGTCGTTAAACACTTCTATCCCAAATTTTTTGTAATTGGAACTAATTCCAACGCCCAAGTTGTTGCCTGTCTTGCTTCCAACATTTTTGTGGAAATACAATGAGCTATAACAGCTCTGCTATGGGTTCTGGTAGTAGAACAGGCAGGATGAGCTTTGAGTTTGGGAGGACCCATGTGGTCAGGCCTAAAGGGAAGCATCAGGCAACTATTGTTTGGTTACATGGCCTTGGTGATAAGGGGTCAAGTTGGTCTCAGCTCTTGGAAACTCTTCCTCTACCAAACATTAAGTGGATTTGTCCTACTGCTCCTACTCGTCCTGTGACGTTATTTGGTGGATTTCCTTGCACTGCATGGTTTGATGTGGGAGATATTTCAGAGGAGTCTCCTGATGATTTAGAAGGCTTGGATGCTGCAGCATCGCATGTTGCTAATCTTTTGTCTACAGAACCTGCTGATATTAAACTGGGTATTGGAGGCTTCAGTATGGGTGCTGCAACTGCTATTTACTCTGCGTCACGCCGTATTCTTGGGCAATATGGAAATGGAAACCTATACCCTATTAACCTTAGTGCAGTTGTTGGGCTTAGTGGCTGGCTTCCCTGTTCAAGATCTTTGAGGAACCAGGTCAGTGTGTCACACGAAGCTGCAAGGCGCGCTGCGTCCCTGCCCATTTTACTCTGTCATGGTTCAGGTGACGATGTCGTAGCTTACAAACACGGGGAGAAATCTGCACATACATTAAGTTCATCTGGATTTAGCAATCTAACATTTAAATCCTATAACGGGCTGGGCCATTACACCATTCCTGAGGAGATGGATGTTGTTTGTAATTGGCTGACAGTCGTTTTGGGGCTCGATGGCTTACGGTTGGTCGATCACTGA

Coding sequence (CDS)

ATGAGCTATAACAGCTCTGCTATGGGTTCTGGTAGTAGAACAGGCAGGATGAGCTTTGAGTTTGGGAGGACCCATGTGGTCAGGCCTAAAGGGAAGCATCAGGCAACTATTGTTTGGTTACATGGCCTTGGTGATAAGGGGTCAAGTTGGTCTCAGCTCTTGGAAACTCTTCCTCTACCAAACATTAAGTGGATTTGTCCTACTGCTCCTACTCGTCCTGTGACGTTATTTGGTGGATTTCCTTGCACTGCATGGTTTGATGTGGGAGATATTTCAGAGGAGTCTCCTGATGATTTAGAAGGCTTGGATGCTGCAGCATCGCATGTTGCTAATCTTTTGTCTACAGAACCTGCTGATATTAAACTGGGTATTGGAGGCTTCAGTATGGGTGCTGCAACTGCTATTTACTCTGCGTCACGCCGTATTCTTGGGCAATATGGAAATGGAAACCTATACCCTATTAACCTTAGTGCAGTTGTTGGGCTTAGTGGCTGGCTTCCCTGTTCAAGATCTTTGAGGAACCAGGTCAGTGTGTCACACGAAGCTGCAAGGCGCGCTGCGTCCCTGCCCATTTTACTCTGTCATGGTTCAGGTGACGATGTCGTAGCTTACAAACACGGGGAGAAATCTGCACATACATTAAGTTCATCTGGATTTAGCAATCTAACATTTAAATCCTATAACGGGCTGGGCCATTACACCATTCCTGAGGAGATGGATGTTGTTTGTAATTGGCTGACAGTCGTTTTGGGGCTCGATGGCTTACGGTTGGTCGATCACTGA

Protein sequence

MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADIKLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSHEAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMDVVCNWLTVVLGLDGLRLVDH

Homology

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BLAST of Carg09324-RA vs. NCBI nr
Match: XP_022961761.1 (acyl-protein thioesterase 2-like [Cucurbita moschata])

HSP 1 Score: 533.5 bits (1373), Expect = 4.0e-148
Identity = 259/260 (99.62%), Postives = 259/260 (99.62%), Query Frame = 0


Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLGIGGFSMGAATAIYSAS RILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD
Sbjct: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVVLGLDGLRLVDH 261
           VVCNWLTVVLGLDGLRLVDH
Sbjct: 241 VVCNWLTVVLGLDGLRLVDH 260

[Close]

BLAST of Carg09324-RA vs. NCBI nr
Match: XP_022996560.1 (acyl-protein thioesterase 2-like [Cucurbita maxima] >XP_022996561.1 acyl-protein thioesterase 2-like [Cucurbita maxima] >XP_022996562.1 acyl-protein thioesterase 2-like [Cucurbita maxima] >XP_022996563.1 acyl-protein thioesterase 2-like [Cucurbita maxima])

HSP 1 Score: 528.5 bits (1360), Expect = 1.3e-146
Identity = 255/260 (98.08%), Postives = 257/260 (98.85%), Query Frame = 0


Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSS MGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSTMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLGIGGFSMGAATAIYSAS RILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD
Sbjct: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVVLGLDGLRLVDH 261
           VVCNWLTV+L LDG+RLVDH
Sbjct: 241 VVCNWLTVILALDGIRLVDH 260

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BLAST of Carg09324-RA vs. NCBI nr
Match: XP_023545446.1 (acyl-protein thioesterase 2-like [Cucurbita pepo subsp. pepo] >XP_023545447.1 acyl-protein thioesterase 2-like [Cucurbita pepo subsp. pepo] >XP_023545448.1 acyl-protein thioesterase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 525.4 bits (1352), Expect = 1.1e-145
Identity = 254/260 (97.69%), Postives = 256/260 (98.46%), Query Frame = 0


Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSS MGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSTMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLGIGGFSMGAATAIYSAS RILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVS+SH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSMSH 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGF NLTFKSYNGLGHYTIPEEMD
Sbjct: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFRNLTFKSYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVVLGLDGLRLVDH 261
           VVCNWLTVVL LDG+RLVDH
Sbjct: 241 VVCNWLTVVLALDGVRLVDH 260

[Close]

BLAST of Carg09324-RA vs. NCBI nr
Match: XP_004138733.1 (PREDICTED: acyl-protein thioesterase 2 [Cucumis sativus] >XP_011649812.1 PREDICTED: acyl-protein thioesterase 2 [Cucumis sativus] >XP_011649813.1 PREDICTED: acyl-protein thioesterase 2 [Cucumis sativus] >XP_011649814.1 PREDICTED: acyl-protein thioesterase 2 [Cucumis sativus] >KGN62923.1 hypothetical protein Csa_2G380030 [Cucumis sativus])

HSP 1 Score: 515.4 bits (1326), Expect = 1.1e-142
Identity = 245/260 (94.23%), Postives = 255/260 (98.08%), Query Frame = 0


Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSS +GSGSRTGRM+FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQ+LETLPLP
Sbjct: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQILETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV LFGGFPCTAWFDVGDISE+SPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLGIGGFSMGAATAIYSAS RILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQ++VSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAA LPILLCHGSGDDVVAYKHGEKSAHTLSS+GF NLTFK+YNGLGHYTIPEEM+
Sbjct: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240

Query: 241 VVCNWLTVVLGLDGLRLVDH 261
           VVCNWLTV+LGLDGLRLVDH
Sbjct: 241 VVCNWLTVILGLDGLRLVDH 260

[Close]

BLAST of Carg09324-RA vs. NCBI nr
Match: XP_008445177.1 (PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo] >XP_008445178.1 PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo] >XP_008445179.1 PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo] >XP_008445180.1 PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo] >XP_016899931.1 PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo])

HSP 1 Score: 513.5 bits (1321), Expect = 4.3e-142
Identity = 245/260 (94.23%), Postives = 252/260 (96.92%), Query Frame = 0


Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSS +GSGSRTGRM+FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV LFGGFPCTAWFDVGDISE+SPDDLEGLDAAASHVANLLSTEP DI
Sbjct: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPGDI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLGIGGFSMGAATAIYSAS RILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQ+ VSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQIGVSH 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAA RAASLPILLCHGSGDDVVAYKHGEKSAHTLSS+GF NLTFK+YNGLGHYTIPEEMD
Sbjct: 181 EAAMRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKAYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVVLGLDGLRLVDH 261
           VVC WLTV+LGLDGLRLVDH
Sbjct: 241 VVCQWLTVILGLDGLRLVDH 260

[Close]

BLAST of Carg09324-RA vs. TAIR10
Match: AT5G20060.2 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 373.6 bits (958), Expect = 9.7e-104
Identity = 174/254 (68.50%), Postives = 212/254 (83.46%), Query Frame = 0


Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MS + +A+GSG R  R + EFG+THVVRPKGKHQATIVWLHGLGD GSSWSQLLETLPLP
Sbjct: 1   MSISGAAVGSG-RNLRRAVEFGKTHVVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAP++P++LFGGFP TAWFDV DI+E+ PDD+EGLD AA+HVANLLS EPADI
Sbjct: 61  NIKWICPTAPSQPISLFGGFPSTAWFDVVDINEDGPDDMEGLDVAAAHVANLLSNEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLG+GGFSMGAAT++YSA+   LG+YGNGN YPINLSA++GLSGWLPC+++L  ++    
Sbjct: 121 KLGVGGFSMGAATSLYSATCFALGKYGNGNPYPINLSAIIGLSGWLPCAKTLAGKLE-EE 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           +   RAASLPI++CHG  DDVV +K GEKS+  L S+GF  +TFK Y+ LGH+TIP+E+D
Sbjct: 181 QIKNRAASLPIVVCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQELD 240

Query: 241 VVCNWLTVVLGLDG 255
            +C WLT  L L+G
Sbjct: 241 ELCAWLTSTLSLEG 252

[Close]

BLAST of Carg09324-RA vs. TAIR10
Match: AT1G52700.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 372.9 bits (956), Expect = 1.7e-103
Identity = 178/256 (69.53%), Postives = 212/256 (82.81%), Query Frame = 0


Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSY+  +MGSGSR  R  +EFGRT+VVRPKGKHQAT+VWLHGLGD GSS SQL+++L LP
Sbjct: 1   MSYSHQSMGSGSRNAR-GYEFGRTYVVRPKGKHQATLVWLHGLGDNGSSSSQLMDSLHLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAP+RPVT  GGF CTAWFDVG+ISE+  DDLEGLDA+ASH+ANLLS+EPAD+
Sbjct: 61  NIKWICPTAPSRPVTSLGGFTCTAWFDVGEISEDGHDDLEGLDASASHIANLLSSEPADV 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           K+GIGGFSMGAA ++YSA+   LG+YG G+ YPINL AVVGLSGWLP  +SLR+++  S 
Sbjct: 121 KVGIGGFSMGAAISLYSATCYALGRYGTGHAYPINLQAVVGLSGWLPGWKSLRSKIECSF 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAASLPI+L HG+ DDVV Y+ GEKSA +L  +GF    FK Y GLGHYT+P EMD
Sbjct: 181 EAARRAASLPIILTHGTSDDVVPYRFGEKSAQSLGMAGFRLAMFKPYEGLGHYTVPREMD 240

Query: 241 VVCNWLTVVLGLDGLR 257
            V +WLT +LGL+G R
Sbjct: 241 EVVHWLTTMLGLEGSR 255

[Close]

BLAST of Carg09324-RA vs. TAIR10
Match: AT3G15650.2 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 362.1 bits (928), Expect = 2.9e-100
Identity = 178/275 (64.73%), Postives = 212/275 (77.09%), Query Frame = 0


Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGS------------ 60
           MSY+  +MGSGSR+ R  +EFGRT+VVRPKGKHQATIVWLHGLGD GS            
Sbjct: 1   MSYSRQSMGSGSRSTR-GYEFGRTYVVRPKGKHQATIVWLHGLGDNGSRILACSLITTSH 60

Query: 61  -------SWSQLLETLPLPNIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEG 120
                  S SQLLE+LPLPNIKWICPTAP+RPV+L GGFPCTAWFDVG+ISE+  DD+EG
Sbjct: 61  FGSVSFCSSSQLLESLPLPNIKWICPTAPSRPVSLLGGFPCTAWFDVGEISEDLHDDIEG 120

Query: 121 LDAAASHVANLLSTEPADIKLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVG 180
           LDA+A+H+ANLLS EP D+K+GIGGFSMGAA A+YS +   LG+YG G+ Y INL A VG
Sbjct: 121 LDASAAHIANLLSAEPTDVKVGIGGFSMGAAIALYSTTCYALGRYGTGHAYTINLRATVG 180

Query: 181 LSGWLPCSRSLRNQVSVSHEAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSN 240
           LSGWLP  RSLR+++  S+E ARRAAS+PILL HG+ DDVV Y+ GEKSAH+L+ +GF  
Sbjct: 181 LSGWLPGWRSLRSKIESSNEVARRAASIPILLAHGTSDDVVPYRFGEKSAHSLAMAGFRQ 240

Query: 241 LTFKSYNGLGHYTIPEEMDVVCNWLTVVLGLDGLR 257
             FK Y GLGHYT+P+EMD V +WL   LGL+G R
Sbjct: 241 TMFKPYEGLGHYTVPKEMDEVVHWLVSRLGLEGSR 274

[Close]

BLAST of Carg09324-RA vs. TAIR10
Match: AT1G52695.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 160.6 bits (405), Expect = 1.3e-39
Identity = 93/223 (41.70%), Postives = 123/223 (55.16%), Query Frame = 0


Query: 8   MGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICP 67
           M SGS       EFG+ + V P G H+ATIVWLH +G+ G +  + L+ L LPNIKWICP
Sbjct: 1   MASGS-INVSGLEFGQINTVYPTGIHKATIVWLHDVGNTGFNSLEPLQNLRLPNIKWICP 60

Query: 68  TAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADIKLGIGGF 127
           TAP R VT  GG    AW D+  +SE   DD   L+    ++ +L S EP ++  G+ G 
Sbjct: 61  TAPRRRVTSLGGEITNAWCDIAKVSENMQDDFGTLNYVNEYITSLFSNEPQNVIKGVAGL 120

Query: 128 SMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLR-NQVSVSHEAARRA 187
            +GAA A+Y  S    G        PIN   V+G++GWLP  R L  N  + +   A RA
Sbjct: 121 GLGAAQALYYTSCYAFG------WVPINPQIVIGINGWLPGWRRLEYNMNNTNFGTANRA 180

Query: 188 ASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNG 230
           A+  IL+ HG+ DDVV    G + A +L  +GF  L FK   G
Sbjct: 181 AASKILILHGTSDDVVPSSFGYRCADSLRMAGFPTL-FKQCGG 215

[Close]

BLAST of Carg09324-RA vs. TAIR10
Match: AT1G52470.1 (alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 129.8 bits (325), Expect = 2.4e-30
Identity = 81/241 (33.61%), Postives = 122/241 (50.62%), Query Frame = 0


Query: 10  SGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICPTA 69
           S SR G    +   +H V P+G  +A+IVWLH   +  +   Q +++L L N+ WICP  
Sbjct: 4   SSSRNGANVLDLKCSHFVEPQGDQRASIVWLHDKDEHFTDSVQFVKSLKLKNVNWICP-- 63

Query: 70  PTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADIKLGIGGFSM 129
              P+           +D G  S    DD E LD+AA  VA+LL  EP ++  G+GGF M
Sbjct: 64  ---PIVY-----TNTSYDFG--SNIKQDDREALDSAAKFVADLLLREPLNVVKGVGGFGM 123

Query: 130 GAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSHEAARRAASL 189
           GA  A+  A+   LG       YPIN   VVG++GWL  + S+ + +  +  A  RAAS 
Sbjct: 124 GAVVALQFATNCALGH------YPINPRVVVGINGWLSITGSITSSIEYTVGAVARAASQ 183

Query: 190 PILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMDVVCNWLTVV 249
            I    G+ + ++ Y   E+   +L  +GF ++ F  Y+ L H    +  D++  WL + 
Sbjct: 184 KIFFTRGAENRLLPYTREEEVVESLREAGFGDVFFLIYSWLRHEHHLDIRDMLKLWLELS 226

Query: 250 L 251
           L
Sbjct: 244 L 226

[Close]

BLAST of Carg09324-RA vs. Swiss-Prot
Match: sp|O95372|LYPA2_HUMAN (Acyl-protein thioesterase 2 OS=Homo sapiens OX=9606 GN=LYPLA2 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.7e-28
Identity = 79/225 (35.11%), Postives = 120/225 (53.33%), Query Frame = 0


Query: 32  KHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICPTAPTRPVTLFGGFPCTAWFDVGDI 91
           +  A +++LHGLGD G SW+  L T+ LP++K+ICP AP  PVTL       +WFD+  +
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 92  SEESPDDLEGLDAAASHVANLLSTE-----PADIKLGIGGFSMGAATAIYSASRRILGQY 151
           S ++P+D  G+  AA ++  L+  E     PA+ ++ +GGFS G A ++Y+A        
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPAN-RIVLGGFSQGGALSLYTAL------- 141

Query: 152 GNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSHEAARRAASLPILLCHGSGDDVVAYKH 211
                 P  L+ +V LS WLP  R+     + S      A  L IL CHG  D +V  + 
Sbjct: 142 ----TCPHPLAGIVALSCWLPLHRAFPQAANGS------AKDLAILQCHGELDPMVPVRF 201

Query: 212 GEKSAHTLSSSGF-SNLTFKSYNGLGHYTIPEEMDVVCNWLTVVL 251
           G  +A  L S    + + FK+Y G+ H + P+EM  V  +L  +L
Sbjct: 202 GALTAEKLRSVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

[Close]

BLAST of Carg09324-RA vs. Swiss-Prot
Match: sp|Q9WTL7|LYPA2_MOUSE (Acyl-protein thioesterase 2 OS=Mus musculus OX=10090 GN=Lypla2 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.7e-28
Identity = 78/225 (34.67%), Postives = 120/225 (53.33%), Query Frame = 0


Query: 32  KHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICPTAPTRPVTLFGGFPCTAWFDVGDI 91
           +  A +++LHGLGD G SW+  L T+ LP++K+ICP AP  PVTL       +WFD+  +
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 92  SEESPDDLEGLDAAASHVANLLSTE-----PADIKLGIGGFSMGAATAIYSASRRILGQY 151
           S ++P+D  G+  AA ++  L+  E     PA+ ++ +GGFS G A ++Y+A        
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPAN-RIVLGGFSQGGALSLYTAL------- 141

Query: 152 GNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSHEAARRAASLPILLCHGSGDDVVAYKH 211
                 P  L+ +V LS WLP  R+     + S      A  L IL CHG  D +V  + 
Sbjct: 142 ----TCPHPLAGIVALSCWLPLHRNFPQAANGS------AKDLAILQCHGELDPMVPVRF 201

Query: 212 GEKSAHTLSSSGF-SNLTFKSYNGLGHYTIPEEMDVVCNWLTVVL 251
           G  +A  L +    + + FK+Y G+ H + P+EM  V  +L  +L
Sbjct: 202 GALTAEKLRTVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

[Close]

BLAST of Carg09324-RA vs. Swiss-Prot
Match: sp|Q9QYL8|LYPA2_RAT (Acyl-protein thioesterase 2 OS=Rattus norvegicus OX=10116 GN=Lypla2 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.7e-28
Identity = 78/225 (34.67%), Postives = 120/225 (53.33%), Query Frame = 0


Query: 32  KHQATIVWLHGLGDKGSSWSQLLETLPLPNIKWICPTAPTRPVTLFGGFPCTAWFDVGDI 91
           +  A +++LHGLGD G SW+  L T+ LP++K+ICP AP  PVTL       +WFD+  +
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 92  SEESPDDLEGLDAAASHVANLLSTE-----PADIKLGIGGFSMGAATAIYSASRRILGQY 151
           S ++P+D  G+  AA ++  L+  E     PA+ ++ +GGFS G A ++Y+A        
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPAN-RIVLGGFSQGGALSLYTAL------- 141

Query: 152 GNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSHEAARRAASLPILLCHGSGDDVVAYKH 211
                 P  L+ +V LS WLP  R+     + S      A  L IL CHG  D +V  + 
Sbjct: 142 ----TCPHPLAGIVALSCWLPLHRNFPQAANGS------AKDLAILQCHGELDPMVPVRF 201

Query: 212 GEKSAHTLSSSGF-SNLTFKSYNGLGHYTIPEEMDVVCNWLTVVL 251
           G  +A  L +    + + FK+Y G+ H + P+EM  V  +L  +L
Sbjct: 202 GALTAEKLRTVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

[Close]

BLAST of Carg09324-RA vs. Swiss-Prot
Match: sp|Q54T49|APT11_DICDI (Acyl-protein thioesterase 1 homolog 1 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0282005 PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 5.9e-26
Identity = 70/227 (30.84%), Postives = 119/227 (52.42%), Query Frame = 0


Query: 33  HQATIVWLHGLGDKGSSWSQLLETLPLPN---IKWICPTAPTRPVTLFGGFPCTAWFDVG 92
           H AT+++ HGLGD G+ W +++E +   N   I++ICP AP + VTL GGF   +W+D+ 
Sbjct: 18  HSATVIFSHGLGDSGAGWIEVMEEIQSRNNGHIRFICPNAPIQAVTLNGGFKMPSWYDIK 77

Query: 93  DISEESPDDLEGLDAAASHVANLLSTEPADIKLG-----IGGFSMGAATAIYSASRRILG 152
            +S    +D   +D + + +  ++  E  + K+      IGGFS GAA ++Y+   +   
Sbjct: 78  SLSSRGDEDPAQVDESKNIIETIIKHEMEEEKIPAERIIIGGFSQGAALSLYTFYSQT-- 137

Query: 153 QYGNGNLYPINLSAVVGLSGWLP-CSRSLRNQVSVSHEAARRAASLPILLCHGSGDDVVA 212
                      L   + LSG+LP  ++ + N ++            P+L+ HG  D VV 
Sbjct: 138 --------ETKLGGCIALSGYLPLATKFVANSLNKEQ---------PLLMIHGDCDQVVR 197

Query: 213 YKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMDVVCNWLTVVL 251
           ++ G+ S   L S G  N  F +  GLGH++ PEE+D++  +++  L
Sbjct: 198 HQWGKLSFDHLKSQGI-NGEFITLKGLGHHSSPEEIDLMTKFISKTL 224

[Close]

BLAST of Carg09324-RA vs. Swiss-Prot
Match: sp|P0CL95|APTH1_CRYNB (Acyl-protein thioesterase 1 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=CNBF2260 PE=3 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 5.0e-25
Identity = 74/247 (29.96%), Postives = 119/247 (48.18%), Query Frame = 0


Query: 27  VRPKGKHQATIVWLHGLGDKGSSWSQLLETL--PLPNIKWICPTAPTRPVTLFGGFPCTA 86
           + PK  H AT+++LHGLGD G  W  + + L    PN+KWI P APT PV+L  G    +
Sbjct: 10  ISPKEAHTATVIFLHGLGDSGHGWLPVAKMLWSSFPNVKWILPHAPTIPVSLNHGMAMPS 69

Query: 87  WFDV---GDISEESPDDLEGLDAAASHVANLLSTEP----ADIKLGIGGFSMGAATAIYS 146
           WFD+     +     DD +G+      V  L+  E      + ++ +GGFS G A ++ +
Sbjct: 70  WFDIRHLDKLDNSENDDEQGMLETLKSVDELIQAEVDSGIPENRIVLGGFSQGGAISVLN 129

Query: 147 --ASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSHEAARRAASLPILLCH 206
              ++R              L+ VV LS W+P +  +   +S        A  +P+   H
Sbjct: 130 MLTTKR-------------KLAGVVALSTWVPLNHKIVQMMS------EHAKDIPVFWGH 189

Query: 207 GSGDDVVAYKHGEKS--------AHTLSSSGFS----NLTFKSYNGLGHYTIPEEMDVVC 251
           G+ D VV Y+ G++S         + L S G +     + F+SY G+ H + P+E++ + 
Sbjct: 190 GTNDPVVDYRFGQRSVDFLVQKCGYKLLSQGTTFARPGIRFESYPGMPHSSCPQEIEDLK 237

[Close]

BLAST of Carg09324-RA vs. TrEMBL
Match: tr|A0A0A0LM90|A0A0A0LM90_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G380030 PE=4 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 7.5e-143
Identity = 245/260 (94.23%), Postives = 255/260 (98.08%), Query Frame = 0


Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSS +GSGSRTGRM+FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQ+LETLPLP
Sbjct: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQILETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV LFGGFPCTAWFDVGDISE+SPDDLEGLDAAASHVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLGIGGFSMGAATAIYSAS RILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQ++VSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQINVSH 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAA LPILLCHGSGDDVVAYKHGEKSAHTLSS+GF NLTFK+YNGLGHYTIPEEM+
Sbjct: 181 EAARRAACLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKTYNGLGHYTIPEEMN 240

Query: 241 VVCNWLTVVLGLDGLRLVDH 261
           VVCNWLTV+LGLDGLRLVDH
Sbjct: 241 VVCNWLTVILGLDGLRLVDH 260

[Close]

BLAST of Carg09324-RA vs. TrEMBL
Match: tr|A0A1S4DVB8|A0A1S4DVB8_CUCME (acyl-protein thioesterase 2-like OS=Cucumis melo OX=3656 GN=LOC103488285 PE=4 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 2.9e-142
Identity = 245/260 (94.23%), Postives = 252/260 (96.92%), Query Frame = 0


Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYNSS +GSGSRTGRM+FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNSSTVGSGSRTGRMTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV LFGGFPCTAWFDVGDISE+SPDDLEGLDAAASHVANLLSTEP DI
Sbjct: 61  NIKWICPTAPTRPVALFGGFPCTAWFDVGDISEDSPDDLEGLDAAASHVANLLSTEPGDI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLGIGGFSMGAATAIYSAS RILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQ+ VSH
Sbjct: 121 KLGIGGFSMGAATAIYSASCRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQIGVSH 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAA RAASLPILLCHGSGDDVVAYKHGEKSAHTLSS+GF NLTFK+YNGLGHYTIPEEMD
Sbjct: 181 EAAMRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSAGFRNLTFKAYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVVLGLDGLRLVDH 261
           VVC WLTV+LGLDGLRLVDH
Sbjct: 241 VVCQWLTVILGLDGLRLVDH 260

[Close]

BLAST of Carg09324-RA vs. TrEMBL
Match: tr|B9IKU8|B9IKU8_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_018G030200v3 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 1.1e-125
Identity = 215/254 (84.65%), Postives = 237/254 (93.31%), Query Frame = 0


Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYN+SA+GSGSR    +FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNTSAVGSGSRNTTRTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV +FGGFPCTAWFDVGDISE++PDDLEGL+A+A+HVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVAIFGGFPCTAWFDVGDISEDAPDDLEGLEASATHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLG+GGFSMGAATA+YSA+  +LGQYGNGN YPINL+AVVGLSGWLPCSR+LR+++  S 
Sbjct: 121 KLGVGGFSMGAATALYSATCHVLGQYGNGNQYPINLTAVVGLSGWLPCSRTLRSRIERSD 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAASLPILLCHGSGDDVVA+KHGEKSA  LSS+GF NLTF+SYNGLGHYTIPEEMD
Sbjct: 181 EAARRAASLPILLCHGSGDDVVAHKHGEKSAQALSSAGFRNLTFRSYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVVLGLDG 255
            VC+WLT  LGLDG
Sbjct: 241 EVCHWLTTRLGLDG 254

[Close]

BLAST of Carg09324-RA vs. TrEMBL
Match: tr|A0A2K2A818|A0A2K2A818_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_006G251100v3 PE=4 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 1.4e-125
Identity = 215/256 (83.98%), Postives = 235/256 (91.80%), Query Frame = 0


Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYN+SA GSGSR    +FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP
Sbjct: 1   MSYNTSAAGSGSRNTTRTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV +FGGFPCTAW D GDISE++PDDLEGLDA+A+HVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVAIFGGFPCTAWSDAGDISEDAPDDLEGLDASAAHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLG+GGFSMGAATA+YSA+  I GQYGNGNLYP+NLSA+VGLSGWLPCSR+LRN++  S 
Sbjct: 121 KLGVGGFSMGAATALYSATCHIFGQYGNGNLYPVNLSAIVGLSGWLPCSRTLRNRMERSD 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAASLPILLCHGSGDDVVA+KHGEKSA  LSS+GF NLTF+SYNGLGHYTIPEEMD
Sbjct: 181 EAARRAASLPILLCHGSGDDVVAHKHGEKSAQALSSAGFRNLTFRSYNGLGHYTIPEEMD 240

Query: 241 VVCNWLTVVLGLDGLR 257
            VCNWLT  +GL+G R
Sbjct: 241 EVCNWLTTRIGLEGPR 256

[Close]

BLAST of Carg09324-RA vs. TrEMBL
Match: tr|A0A2P5DUI3|A0A2P5DUI3_9ROSA (Phospholipase/carboxylesterase/thioesterase OS=Trema orientalis OX=63057 GN=TorRG33x02_241300 PE=4 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 2.1e-124
Identity = 213/256 (83.20%), Postives = 237/256 (92.58%), Query Frame = 0


Query: 1   MSYNSSAMGSGSRTGRMSFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLP 60
           MSYN+S++GSGSRT R +FEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPL 
Sbjct: 1   MSYNNSSIGSGSRTARRTFEFGRTHVVRPKGKHQATIVWLHGLGDKGSSWSQLLETLPLS 60

Query: 61  NIKWICPTAPTRPVTLFGGFPCTAWFDVGDISEESPDDLEGLDAAASHVANLLSTEPADI 120
           NIKWICPTAPTRPV LFGGFPCTAWFD+GDISE++PDDLEGLDA+A+HVANLLSTEPADI
Sbjct: 61  NIKWICPTAPTRPVALFGGFPCTAWFDMGDISEDAPDDLEGLDASAAHVANLLSTEPADI 120

Query: 121 KLGIGGFSMGAATAIYSASRRILGQYGNGNLYPINLSAVVGLSGWLPCSRSLRNQVSVSH 180
           KLGIGGFSMGAATA+YSA+ R+LG+YGNGNLYP+NLSAVVGLSGWLPCSR+LR+ +  S 
Sbjct: 121 KLGIGGFSMGAATALYSATCRVLGKYGNGNLYPVNLSAVVGLSGWLPCSRTLRSLMEGSV 180

Query: 181 EAARRAASLPILLCHGSGDDVVAYKHGEKSAHTLSSSGFSNLTFKSYNGLGHYTIPEEMD 240
           EAARRAAS+PIL+CHG  DDVVAY HG+KSA TLSS+GF NLTF++YNGLGHYT+PEE D
Sbjct: 181 EAARRAASVPILMCHGLVDDVVAYHHGDKSAQTLSSTGFQNLTFRTYNGLGHYTVPEETD 240

Query: 241 VVCNWLTVVLGLDGLR 257
            VCNWLT  LGLDG R
Sbjct: 241 EVCNWLTANLGLDGSR 256

The following BLAST results are available for this feature:

BLAST of Carg09324-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022961761.1	4.0e-148	99.62	acyl-protein thioesterase 2-like [Cucurbita moschata]	[more]
XP_022996560.1	1.3e-146	98.08	acyl-protein thioesterase 2-like [Cucurbita maxima] >XP_022996561.1 acyl-protein...	[more]
XP_023545446.1	1.1e-145	97.69	acyl-protein thioesterase 2-like [Cucurbita pepo subsp. pepo] >XP_023545447.1 ac...	[more]
XP_004138733.1	1.1e-142	94.23	PREDICTED: acyl-protein thioesterase 2 [Cucumis sativus] >XP_011649812.1 PREDICT...	[more]
XP_008445177.1	4.3e-142	94.23	PREDICTED: acyl-protein thioesterase 2-like [Cucumis melo] >XP_008445178.1 PREDI...	[more]

BLAST of Carg09324-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT5G20060.2	9.7e-104	68.50	alpha/beta-Hydrolases superfamily protein	[more]
AT1G52700.1	1.7e-103	69.53	alpha/beta-Hydrolases superfamily protein	[more]
AT3G15650.2	2.9e-100	64.73	alpha/beta-Hydrolases superfamily protein	[more]
AT1G52695.1	1.3e-39	41.70	alpha/beta-Hydrolases superfamily protein	[more]
AT1G52470.1	2.4e-30	33.61	alpha/beta-Hydrolases superfamily protein	[more]

BLAST of Carg09324-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|O95372\|LYPA2_HUMAN	1.7e-28	35.11	Acyl-protein thioesterase 2 OS=Homo sapiens OX=9606 GN=LYPLA2 PE=1 SV=1	[more]
sp\|Q9WTL7\|LYPA2_MOUSE	3.7e-28	34.67	Acyl-protein thioesterase 2 OS=Mus musculus OX=10090 GN=Lypla2 PE=1 SV=1	[more]
sp\|Q9QYL8\|LYPA2_RAT	3.7e-28	34.67	Acyl-protein thioesterase 2 OS=Rattus norvegicus OX=10116 GN=Lypla2 PE=1 SV=1	[more]
sp\|Q54T49\|APT11_DICDI	5.9e-26	30.84	Acyl-protein thioesterase 1 homolog 1 OS=Dictyostelium discoideum OX=44689 GN=DD...	[more]
sp\|P0CL95\|APTH1_CRYNB	5.0e-25	29.96	Acyl-protein thioesterase 1 OS=Cryptococcus neoformans var. neoformans serotype ...	[more]

BLAST of Carg09324-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LM90\|A0A0A0LM90_CUCSA	7.5e-143	94.23	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G380030 PE=4 SV=1	[more]
tr\|A0A1S4DVB8\|A0A1S4DVB8_CUCME	2.9e-142	94.23	acyl-protein thioesterase 2-like OS=Cucumis melo OX=3656 GN=LOC103488285 PE=4 SV...	[more]
tr\|B9IKU8\|B9IKU8_POPTR	1.1e-125	84.65	Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_018G030200v3 PE=...	[more]
tr\|A0A2K2A818\|A0A2K2A818_POPTR	1.4e-125	83.98	Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_006G251100v3 PE=...	[more]
tr\|A0A2P5DUI3\|A0A2P5DUI3_9ROSA	2.1e-124	83.20	Phospholipase/carboxylesterase/thioesterase OS=Trema orientalis OX=63057 GN=TorR...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016787	hydrolase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR003140	PLipase/COase/thioEstase
IPR029058	AB_hydrolase

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016787	hydrolase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg09324	Carg09324	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg09324-RA	Carg09324-RA-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg09324-RA.exon.1	Carg09324-RA.exon.1	exon
Carg09324-RA.exon.2	Carg09324-RA.exon.2	exon
Carg09324-RA.exon.3	Carg09324-RA.exon.3	exon
Carg09324-RA.exon.4	Carg09324-RA.exon.4	exon
Carg09324-RA.exon.5	Carg09324-RA.exon.5	exon
Carg09324-RA.exon.6	Carg09324-RA.exon.6	exon
Carg09324-RA.exon.7	Carg09324-RA.exon.7	exon
Carg09324-RA.exon.8	Carg09324-RA.exon.8	exon
Carg09324-RA.exon.9	Carg09324-RA.exon.9	exon
Carg09324-RA.exon.10	Carg09324-RA.exon.10	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg09324-RA.five_prime_UTR.1	Carg09324-RA.five_prime_UTR.1	five_prime_UTR
Carg09324-RA.five_prime_UTR.2	Carg09324-RA.five_prime_UTR.2	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg09324-RA.CDS.1	Carg09324-RA.CDS.1	CDS
Carg09324-RA.CDS.2	Carg09324-RA.CDS.2	CDS
Carg09324-RA.CDS.3	Carg09324-RA.CDS.3	CDS
Carg09324-RA.CDS.4	Carg09324-RA.CDS.4	CDS
Carg09324-RA.CDS.5	Carg09324-RA.CDS.5	CDS
Carg09324-RA.CDS.6	Carg09324-RA.CDS.6	CDS
Carg09324-RA.CDS.7	Carg09324-RA.CDS.7	CDS
Carg09324-RA.CDS.8	Carg09324-RA.CDS.8	CDS
Carg09324-RA.CDS.9	Carg09324-RA.CDS.9	CDS

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	G3DSA:3.40.50.1820		coord: 15..251 e-value: 5.6E-62 score: 211.3
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	SSF53474	alpha/beta-Hydrolases	coord: 25..247
IPR003140	Phospholipase/carboxylesterase/thioesterase	PFAM	PF02230	Abhydrolase_2	coord: 25..248 e-value: 6.2E-40 score: 137.2
None	No IPR available	PANTHER	PTHR10655	LYSOPHOSPHOLIPASE-RELATED	coord: 2..254
None	No IPR available	PANTHER	PTHR10655:SF34	SUBFAMILY NOT NAMED	coord: 2..254