Carg20944 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg20944
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	PQ-loop repeat family protein / transmembrane family protein
Location	Cucurbita_argyrosperma_scaffold_141 : 735710 .. 739848 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGGTGGGTATTGTGTGAAGGAGCAGAAGGCATGCGTGGGATGGGTTGAAACGTATTTCAAGGACTGTCTTTGCAATCTGAGAGACGACATGTCGTTCGGGTTCGGCGTTGTAAGTCTCCTCTGTTGGGGCCTCGCTGAGATTCCTCAAATCGTCACCAATTTCCGCACCAAGTCCAGCCATGGTGTCTCCATCCTCTTCCTCCTCACTTGGGTCGCCGGGTACTTCTTCTCTCTCTCTTCCCTCTCTCTCTCTCTCTCCTTAAAACGCCGCCGTTTCATCTCTTTCTTTGTCTGGCAGAGATGCGTTTAATCTGCTGGGCTGCCTTCTGGAACCTGCCACGGTGAGTCACTCGGTCCAACTCGGTTCAACTCGGTCCAACTCGGTTTGGTTTCTTTGTTCTCCACTCATTTCTTTTTTTTTTGCTTTTATTTCAGCTCCCCACTCAGCTCTACACAGCCCTGGTGAGTGCCCTTTTTTTTCCCTAATTTAAAATTAAAAAGTAATTAATTTCTAATATTTTAAAATTTTAAAATTTTAAAAATATATATTTGTGACGGTGATTAGACATGGGTGTCTGCAGCTTTACACAGTGAATACGATAGTATTGGTTCTGCAAAGTGTTTATTATGATTACGTCACTAGAAGCCGCAGCGACCGGAAGACCAAATCTCACCAAACGGTACCGTTTTATTCCCTTTTTTTTTTTTTCTTTTATTTATTTTAAAAATTATGAATAATAATGTTATATGATCGGACTAATTTGTATTACTCAACCCAAATCATAAATTTTATGACGAGTTAGATTATATGATTGAAAAATTTGCTCGATTTGTTGGGTTAATCCGACTATTCGAAAATAGGATTACAATCCGATTTGAAAATTTGCTCGATTTGCAGGTTGATAATTTTTTGGTTGGGTTAACTCGATTGTTTTTTTTTGCTCCGGTTTGCTGGTTGATAATTTGTTGGTTGGGTTAAACAGATCATTTCAAAAATAGGGTTATAATTTAATACTATCCTAATAATTACAATTAAGAATCTTGACTATGTAACCTATTTTAAGAATATGTTGTCTTGTCTGGTAAATTAGTCAATTTAAATATTTAATTATTTAGTTTTTAAAATTTTAAATTTTAAATTTTTTTTATTAGATTAAAATAAAATAAAAATAAAGAATAGACCAAAAGTAAAAGTAATTGTTTTGTATTATGACAATGAAAGGCGGCGGAAGAGGAAAACACGCCGTTAAAAGGCAGTAAAGGCGTTAGCTACGCCGGAATTCCAATTCCCAAGGCTTCCCCGAAGGCCACTCCTCGCCGGGAATTCTACTTCACGTCAGCTCGGTCGTTGGCCGGCAGCGATACGCCGCCGTTTAGAGCGTTTCTCCGGTTACCGAAAAGCGAACCCTCCGCCCTCGGGAACGACAGCTCATCTTCGGACGATGAATCCGAAACTGCGGCCGTGTCGTCCAACTCAGCTTTTACCCAGCCGAGGCCCATTCCTCGATCGGTTACCTCTCTTACTTACCTTCAGTCATTCAGCTTCAGTAATTAAAAATTACTGTTCCTTTGGTTTGGTTGTTATTATATTGAAATGATTTGGCATGTTTCAGGTTGGTTATGGCACGTTTTTGGCAGCTTCAGCAAATTTGCCTTTCCAAAGCAAAGGCTTCTCAGATGGATTTAGAGGAAGGAAGCTTTTACAAGTAAGTATTTATGATTTATGATTTATGATTTTCATTTTAACAAATAAATTAAATTATGATTTGGGTAATTTGTTTTAGTGGTTTGCGTTGGTTGGGTTGGGTCGGGTTGGGTCGGGTCGGGTTCAATTGTTCATGTTGTAAATGTGAGATCCTACCTCAGTTGGAGAGGGGAACGAAACATTGCTTATAAGTACGTGAAAACCTTTCTCTAGGTGACATTTTTTAGAATTGTGAGGCTAACGGTACTACGTAATGGGCGAAAGTGGACAATATTTGCTAGCGGTGGGCTTGGACGGTTGCAAATGGTATCAAAGCCAGACACTGGCAGTGTACTAGCAAGGACGTTGGGCTCCCAAAGGGGTAGATTGTGAGATCTCACATCGGTTGGATAGGGGAACGAAGCATTGCTTATAAGGGTGTGGAAACCTCTCCTTATTAGACACGTTTTAAAACCGTGAGACTGGTGACAATACGTAATGGGTCAAAGCAGACAATATTTATTAGCGGTGAGTTTGGGCTGTTATAAAATCGTGATACTGACGATACGTAATGGGCCAAAATGAACAATATTTGCTAATGGGACAATATTTGCCAGTGGTGAGCTTAGACTGTTACAAATGGTGTCAGAGCAAGACAGTGTGCGGTGTGCCAACGAGGAAGTTGGGTCTTCAAAGAGGTGGATTGTGAGATCCCACATCGGTTGGAAAAGTGAACAAAGCATTACTTATAAGGGCGTGGAAACCCTTCTCTACTATACGCGTTTTAAAACCGTGAGACTAACGACAATACGTAACGGGGCAAAGCAGACTATTTTTGTTAGCGGTGGGTTTCGGCTATTACAAAATCGTGATACTGACGATAACGGGCCAAAACGGACAATATTTACTAACAGTAGGTTTGGATGGTTACAAATGGTACCAGAACGGGCCGAAACGGACAATATTTGCTAACGGTAGGTTTAGACTGTTACAAATGGTACCAGAACAGTCTAATGCGGACAATATTTGCTAACGGTAGGCTTGGATGGTTACAAATGGTACTAGCGTTAGACACCAGACAATATGCCGGCGAGGACACTAGCCTCTAATGGAGAGTGGATTGTGAGATTCTACATCAGTTGGAGAGGGGAACGAAACATGTTATAAGAGTGTGAAAACCTCTCCCTAGTTTTTAATACTGAAAGGCTAACGGCGATACGCAATGGGCTAAAGCTAACAATACTTGCTAACGGTGGGTTTGAGCTGTTATACTAAATTTTTCAATCAAAACCAGATATAATTGTACCGCAGGTAAAAGTTATTTTTGAAAATCAACCATAAATTCAAGTGGGTTTAATTTAACTTAATCTAGTTTTAGCGGAACCCATAAACTAATTCAACTCAAATAAGTTTATAATTGAATCCAACATCAAATTTTTCGGGTTATTGAATTATTTAACGTGTATTTACAGGAACATGCGACCCATAGCGGGTTTGGGCAATTCTTGGGTTGGCTTATGGCTGCAATTTACATGGGTGGTAGACTCCCTCAGATTTGGTTAAATGTGGGTATCAAATTTTGAATCTTTTATTCTTTTTAATTTTTATTATTATCCTCAAATTTATTTATTTTATTTTCTTGCCAGATCAAAAGAGGAAGTGTGGAGGGGCTGAATCCCCTCATGTTCGTGTTTGCGTTGGTTGCCAATGCCACTTATGTAGCAAGGTAAGGTTCGTTCTCTCGATACCGACCTCGATATCAATTCCGGGTTTTTTTAATATCGTCCTCTCGTTGAATGAATGCAGTATTGTCGTGAGAAGTACGGAATGGGATAAAATCAAAGCTAACATGCCGTGGCTGCTCGATGCCGTTGTTTGCGTCGTCCTCGACTTATTCGTATCCTTTTTCGTGATCCTTTTACACTATGATTTGGTTTGAAATGTGAAAGCCTTAACTCAAACACCCAATAGATAATTGTGCAGTATATATATTACAGAAGTTTTAGAAGGAAGAGGCAAAGTGGAGGAAGAGAGTATGAAGAAGCAACAAAACATGCAACCTTGTGACCAATTTAAGTAAAAATAATGCCTTTTCTTCTTCATTTCTTGCATTCAGTGGCACAAAATGGGTCCTTTTGGCCTCTTTACTGAGTGATTCAAGAGGCTGCTAATGGAAGGTTGAATATTCTTTGTACTGTTCTTGAAGTATTCTAGACTTGTTTTTAGTTTTAAACTCTTGAAAGGGTGTTGTGATGATCCTTTATGGGAGTTTTATGTTTGTGTTGTTTGGAGTTGAAATGCAAAGAATGAATTTGATTTGGGTTTCCAAAATAAAGCCCAAAAGCCCAATGAAATGGTCCCTATGAGACTATGGAAGATAGAAATATCGACTTTTGAGATGATAATAGTTACTTTATTCTCTAAGCTATTATCGAACTGGTACTCGGTAT

mRNA sequence

ATGAAGGGTGGGTATTGTGTGAAGGAGCAGAAGGCATGCGTGGGATGGGTTGAAACGTATTTCAAGGACTGTCTTTGCAATCTGAGAGACGACATGTCGTTCGGGTTCGGCGTTGTAAGTCTCCTCTGTTGGGGCCTCGCTGAGATTCCTCAAATCGTCACCAATTTCCGCACCAAGTCCAGCCATGGTGTCTCCATCCTCTTCCTCCTCACTTGGGTCGCCGGAGATGCGTTTAATCTGCTGGGCTGCCTTCTGGAACCTGCCACGCTCCCCACTCAGCTCTACACAGCCCTGCTTTACACAGTGAATACGATAGTATTGGTTCTGCAAAGTGTTTATTATGATTACGTCACTAGAAGCCGCAGCGACCGGAAGACCAAATCTCACCAAACGGCGGCGGAAGAGGAAAACACGCCGTTAAAAGGCAGTAAAGGCGTTAGCTACGCCGGAATTCCAATTCCCAAGGCTTCCCCGAAGGCCACTCCTCGCCGGGAATTCTACTTCACGTCAGCTCGGTCGTTGGCCGGCAGCGATACGCCGCCGTTTAGAGCGTTTCTCCGGTTACCGAAAAGCGAACCCTCCGCCCTCGGGAACGACAGCTCATCTTCGGACGATGAATCCGAAACTGCGGCCGTGTCGTCCAACTCAGCTTTTACCCAGCCGAGGCCCATTCCTCGATCGGTTGGTTATGGCACGTTTTTGGCAGCTTCAGCAAATTTGCCTTTCCAAAGCAAAGGCTTCTCAGATGGATTTAGAGGAAGGAAGCTTTTACAAATCAAAAGAGGAAGTGTGGAGGGGCTGAATCCCCTCATGTTCGTGTTTGCGTTGGTTGCCAATGCCACTTATGTAGCAAGTATTGTCGTGAGAAGTACGGAATGGGATAAAATCAAAGCTAACATGCCGTGGCTGCTCGATGCCGTTGTTTGCGTCGTCCTCGACTTATTCATAATTGTGCAGTATATATATTACAGAAGTTTTAGAAGGAAGAGGCAAAGTGGAGGAAGAGAGTATGAAGAAGCAACAAAACATGCAACCTTGTGACCAATTTAAGTAAAAATAATGCCTTTTCTTCTTCATTTCTTGCATTCAGTGGCACAAAATGGGTCCTTTTGGCCTCTTTACTGAGTGATTCAAGAGGCTGCTAATGGAAGGTTGAATATTCTTTGTACTGTTCTTGAAGTATTCTAGACTTGTTTTTAGTTTTAAACTCTTGAAAGGGTGTTGTGATGATCCTTTATGGGAGTTTTATGTTTGTGTTGTTTGGAGTTGAAATGCAAAGAATGAATTTGATTTGGGTTTCCAAAATAAAGCCCAAAAGCCCAATGAAATGGTCCCTATGAGACTATGGAAGATAGAAATATCGACTTTTGAGATGATAATAGTTACTTTATTCTCTAAGCTATTATCGAACTGGTACTCGGTAT

Coding sequence (CDS)

ATGAAGGGTGGGTATTGTGTGAAGGAGCAGAAGGCATGCGTGGGATGGGTTGAAACGTATTTCAAGGACTGTCTTTGCAATCTGAGAGACGACATGTCGTTCGGGTTCGGCGTTGTAAGTCTCCTCTGTTGGGGCCTCGCTGAGATTCCTCAAATCGTCACCAATTTCCGCACCAAGTCCAGCCATGGTGTCTCCATCCTCTTCCTCCTCACTTGGGTCGCCGGAGATGCGTTTAATCTGCTGGGCTGCCTTCTGGAACCTGCCACGCTCCCCACTCAGCTCTACACAGCCCTGCTTTACACAGTGAATACGATAGTATTGGTTCTGCAAAGTGTTTATTATGATTACGTCACTAGAAGCCGCAGCGACCGGAAGACCAAATCTCACCAAACGGCGGCGGAAGAGGAAAACACGCCGTTAAAAGGCAGTAAAGGCGTTAGCTACGCCGGAATTCCAATTCCCAAGGCTTCCCCGAAGGCCACTCCTCGCCGGGAATTCTACTTCACGTCAGCTCGGTCGTTGGCCGGCAGCGATACGCCGCCGTTTAGAGCGTTTCTCCGGTTACCGAAAAGCGAACCCTCCGCCCTCGGGAACGACAGCTCATCTTCGGACGATGAATCCGAAACTGCGGCCGTGTCGTCCAACTCAGCTTTTACCCAGCCGAGGCCCATTCCTCGATCGGTTGGTTATGGCACGTTTTTGGCAGCTTCAGCAAATTTGCCTTTCCAAAGCAAAGGCTTCTCAGATGGATTTAGAGGAAGGAAGCTTTTACAAATCAAAAGAGGAAGTGTGGAGGGGCTGAATCCCCTCATGTTCGTGTTTGCGTTGGTTGCCAATGCCACTTATGTAGCAAGTATTGTCGTGAGAAGTACGGAATGGGATAAAATCAAAGCTAACATGCCGTGGCTGCTCGATGCCGTTGTTTGCGTCGTCCTCGACTTATTCATAATTGTGCAGTATATATATTACAGAAGTTTTAGAAGGAAGAGGCAAAGTGGAGGAAGAGAGTATGAAGAAGCAACAAAACATGCAACCTTGTGA

Protein sequence

MKGGYCVKEQKACVGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKSSHGVSILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRSRSDRKTKSHQTAAEEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTPPFRAFLRLPKSEPSALGNDSSSSDDESETAAVSSNSAFTQPRPIPRSVGYGTFLAASANLPFQSKGFSDGFRGRKLLQIKRGSVEGLNPLMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYRSFRRKRQSGGREYEEATKHATL

Homology

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BLAST of Carg20944 vs. NCBI nr
Match: XP_022927971.1 (probable vacuolar amino acid transporter YPQ3 [Cucurbita moschata])

HSP 1 Score: 655.2 bits (1689), Expect = 1.2e-184
Identity = 343/377 (90.98%), Postives = 346/377 (91.78%), Query Frame = 0


Query: 1   MKGGYCVKEQKACVGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKS 60
           MKGGYCVKEQKACVGWVETYFKDCLCN+RDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKS
Sbjct: 1   MKGGYCVKEQKACVGWVETYFKDCLCNMRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKS 60

Query: 61  SHGVSILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRS 120
           SHGVSILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRS
Sbjct: 61  SHGVSILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRS 120

Query: 121 RSDRKTKSHQTAAEEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTP 180
           RSDRKTKSHQTAAEEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTP
Sbjct: 121 RSDRKTKSHQTAAEEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTP 180

Query: 181 PFRAFLRLPKSEPSALGNDSSSXXXXXETAAVSSNSAFTQPRPIPRSVGYGTFLAASANL 240
           PFRAFLRLPKSEPSALGNDSSSXXXXXETAAVSSNSAFTQPRPIPRSVGYGTFLAASANL
Sbjct: 181 PFRAFLRLPKSEPSALGNDSSSXXXXXETAAVSSNSAFTQPRPIPRSVGYGTFLAASANL 240

Query: 241 PFQSKGFSDGFRGRKLLQ-------------------------------IKRGSVEGLNP 300
           PFQSKGFSDGFRGRKLL+                               IKRGSVEGLNP
Sbjct: 241 PFQSKGFSDGFRGRKLLEEHATHSGFGQFLGWLMAAIYMGGRLPQIWLNIKRGSVEGLNP 300

Query: 301 LMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYRSFRRK 347
           LMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYRSFRRK
Sbjct: 301 LMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYRSFRRK 360

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BLAST of Carg20944 vs. NCBI nr
Match: XP_023512444.1 (probable vacuolar amino acid transporter YPQ3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 645.2 bits (1663), Expect = 1.3e-181
Identity = 339/377 (89.92%), Postives = 340/377 (90.19%), Query Frame = 0


Query: 1   MKGGYCVKEQKACVGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKS 60
           MKGGYCVKEQKACVGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKS
Sbjct: 1   MKGGYCVKEQKACVGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKS 60

Query: 61  SHGVSILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRS 120
           SHGVSILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTR 
Sbjct: 61  SHGVSILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRR 120

Query: 121 RSDRKTKSHQTAAEEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTP 180
           RSDRKTKSHQTAAEEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTP
Sbjct: 121 RSDRKTKSHQTAAEEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTP 180

Query: 181 PFRAFLRLPKSEPSALGNDSSSXXXXXETAAVSSNSAFTQPRPIPRSVGYGTFLAASANL 240
           PFRAFLRLPKSEPSALGNDSS XXXXX   AVSSNS FTQPRPIPRSVGYGTFLAASANL
Sbjct: 181 PFRAFLRLPKSEPSALGNDSSXXXXXXXXXAVSSNSTFTQPRPIPRSVGYGTFLAASANL 240

Query: 241 PFQSKGFSDGFRGRKLLQ-------------------------------IKRGSVEGLNP 300
           PFQSKGFSDGFRGRKLLQ                               IKRGSVEGLNP
Sbjct: 241 PFQSKGFSDGFRGRKLLQEHATHSGFGQFLGWLMAAIYMGGRLPQIWLNIKRGSVEGLNP 300

Query: 301 LMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYRSFRRK 347
           LMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYRSFRRK
Sbjct: 301 LMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYRSFRRK 360

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BLAST of Carg20944 vs. NCBI nr
Match: XP_022971727.1 (probable vacuolar amino acid transporter YPQ3 [Cucurbita maxima])

HSP 1 Score: 632.9 bits (1631), Expect = 6.5e-178
Identity = 333/377 (88.33%), Postives = 334/377 (88.59%), Query Frame = 0


Query: 1   MKGGYCVKEQKACVGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKS 60
           MKGGYCVKEQKACVGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKS
Sbjct: 1   MKGGYCVKEQKACVGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKS 60

Query: 61  SHGVSILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRS 120
           SHGVSILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTR 
Sbjct: 61  SHGVSILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRR 120

Query: 121 RSDRKTKSHQTAAEEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTP 180
           RSDRKTKSHQTAAE ENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTP
Sbjct: 121 RSDRKTKSHQTAAEVENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTP 180

Query: 181 PFRAFLRLPKSEPSALGNDSSSXXXXXETAAVSSNSAFTQPRPIPRSVGYGTFLAASANL 240
           PFRAFLRLPKSEPSALGNDS  XXXXX       NS FTQPRPIPRSVGYGTFLAASANL
Sbjct: 181 PFRAFLRLPKSEPSALGNDSXXXXXXXXXXXXXXNSTFTQPRPIPRSVGYGTFLAASANL 240

Query: 241 PFQSKGFSDGFRGRKLLQ-------------------------------IKRGSVEGLNP 300
           PFQSKGFSDGFRGRKLL+                               IKRGSVEGLNP
Sbjct: 241 PFQSKGFSDGFRGRKLLEEHATHSGFGQFLGWLMAAIYMGGRLPQIWLNIKRGSVEGLNP 300

Query: 301 LMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYRSFRRK 347
           LMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYRSFRRK
Sbjct: 301 LMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYRSFRRK 360

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BLAST of Carg20944 vs. NCBI nr
Match: XP_022155474.1 (probable vacuolar amino acid transporter YPQ1 [Momordica charantia])

HSP 1 Score: 555.4 bits (1430), Expect = 1.3e-154
Identity = 301/379 (79.42%), Postives = 316/379 (83.38%), Query Frame = 0


Query: 3   GGYCVKEQKACVGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKSSH 62
           G YCVKEQK CVGW +TYFKDCLCNLRDDMSF FG +SLLCWGLAEIPQIVTNFRTKSSH
Sbjct: 4   GSYCVKEQKPCVGWADTYFKDCLCNLRDDMSFAFGFISLLCWGLAEIPQIVTNFRTKSSH 63

Query: 63  GVSILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRSRS 122
           GVS+LFLLTWVAGDAFNL+GCLLEPATLPTQLYTALLYTV+TIVLVLQS+YYDYV R  S
Sbjct: 64  GVSLLFLLTWVAGDAFNLVGCLLEPATLPTQLYTALLYTVSTIVLVLQSLYYDYVYRLCS 123

Query: 123 DRKTKSHQTAAEEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTPPF 182
           DRK KS QT  EEE TPLK  K VSYAGIPIPKASPKATPRREFY+TSARSLAGSDTPPF
Sbjct: 124 DRKRKSDQT-GEEEKTPLK-PKAVSYAGIPIPKASPKATPRREFYYTSARSLAGSDTPPF 183

Query: 183 RAFLRLPKSEPSALGNDSSSXXXXXETAAVSSNSAFTQPRPIPRSVGYGTFLAASANLPF 242
           RAFLRLPKS PSALGNDSSSXXXXX  AA    S  ++PRPIPRSVGYGTFLAASANLPF
Sbjct: 184 RAFLRLPKSGPSALGNDSSSXXXXXXXAA----STISRPRPIPRSVGYGTFLAASANLPF 243

Query: 243 QSKGFSDGFRGRKLLQ--------------------------------IKRGSVEGLNPL 302
           QSKGF DGFRGRKLLQ                                IKRGSVEGLNPL
Sbjct: 244 QSKGFPDGFRGRKLLQEHGISHSGFGQLLGWLMAAIYMGGRLPQIWLNIKRGSVEGLNPL 303

Query: 303 MFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYRSFRRKR 347
           MFVFALVANATYVASI+VRST+W KIKANMPWLLDAVVCV+LDLFII+QYIYYR FR+KR
Sbjct: 304 MFVFALVANATYVASILVRSTDWGKIKANMPWLLDAVVCVLLDLFIILQYIYYRRFRKKR 363

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BLAST of Carg20944 vs. NCBI nr
Match: XP_008457880.1 (PREDICTED: probable vacuolar amino acid transporter YPQ1 [Cucumis melo] >XP_008457881.1 PREDICTED: probable vacuolar amino acid transporter YPQ1 [Cucumis melo] >XP_016902196.1 PREDICTED: probable vacuolar amino acid transporter YPQ1 [Cucumis melo])

HSP 1 Score: 552.0 bits (1421), Expect = 1.5e-153
Identity = 293/379 (77.31%), Postives = 307/379 (81.00%), Query Frame = 0


Query: 1   MKGGYCVKEQKACVGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKS 60
           M+G YCVKEQK CVGWVETYFKDCLCNLRDDMSFGFG+VSLLCWGLAEIPQIVTNFRT  
Sbjct: 1   MEGWYCVKEQKRCVGWVETYFKDCLCNLRDDMSFGFGLVSLLCWGLAEIPQIVTNFRTXX 60

Query: 61  SHGVSILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRS 120
                       VAGD FNL+GCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVT+ 
Sbjct: 61  XXXXXXXXXXXXVAGDVFNLVGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTKW 120

Query: 121 RSDRKTKSHQTAAEEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTP 180
             DRK+KS QT  EEE TPLKG+KGV YAGIPIPKASPK TPRREFY+TSARSLAGSDTP
Sbjct: 121 CIDRKSKSDQT-VEEEKTPLKGNKGVGYAGIPIPKASPKPTPRREFYYTSARSLAGSDTP 180

Query: 181 PFRAFLRLPKSEPSALGNDSSSXXXXXETAAVSSNSAFTQPRPIPRSVGYGTFLAASANL 240
           PFRAFLRLPKS PSALGNDSSS     +TAAV S+SA TQPRPIPRSVGYGTFLAASANL
Sbjct: 181 PFRAFLRLPKSGPSALGNDSSSSDEESDTAAVFSHSAVTQPRPIPRSVGYGTFLAASANL 240

Query: 241 PFQSKGFSDGFRGRKLLQ-------------------------------IKRGSVEGLNP 300
           PFQSKGFSDGF GRKLLQ                               IKRGSVEGLNP
Sbjct: 241 PFQSKGFSDGFSGRKLLQEHSSHSGFGQLLGWLMAAIYMGGRLPQIWLNIKRGSVEGLNP 300

Query: 301 LMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYRSFRRK 345
           LMFVFAL+ANATYVASIVVRSTEW+ IKANMPWLLDAVVCV+LDLFII+QYIYYR FRRK
Sbjct: 301 LMFVFALIANATYVASIVVRSTEWENIKANMPWLLDAVVCVLLDLFIILQYIYYRRFRRK 360

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BLAST of Carg20944 vs. TAIR10
Match: AT4G36850.1 (PQ-loop repeat family protein / transmembrane family protein)

HSP 1 Score: 392.9 bits (1008), Expect = 2.1e-109
Identity = 217/385 (56.36%), Postives = 261/385 (67.79%), Query Frame = 0


Query: 4   GYCVKEQKACVGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKSSHG 63
           GYC+KE+K CV WVE YF DCLCNL DD+SF  G+ SLLCWG+AEIPQ++TNFRTKSS+G
Sbjct: 5   GYCLKEKKTCVRWVEIYFDDCLCNLNDDVSFALGIASLLCWGVAEIPQVITNFRTKSSNG 64

Query: 64  VSILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRSRSD 123
           VS+ FLL WVAGD FNL+GCLLEPATLPTQ YTALLYTV+T+VLV+Q++YYDY+ +    
Sbjct: 65  VSLSFLLAWVAGDIFNLVGCLLEPATLPTQFYTALLYTVSTVVLVIQTIYYDYIYKLCRH 124

Query: 124 RKTKSHQTAAE-EENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTPPF 183
           R+TK  Q   E EE  PLK  K +  A I IP  S K + RREFY+TSARSLAGS TPP 
Sbjct: 125 RRTKICQKDEEDEEKRPLKPPKTMGSA-ISIPGGSYKDSSRREFYYTSARSLAGSGTPPL 184

Query: 184 R-AFLRLPKSEPSALGND----SSSXXXXXETAAVSSNSAFTQPRPIPRSVGYGTFLAAS 243
           R ++ R+ KS PSAL  D    SS       T  V +    T+PRPIPR  G+GTFLAAS
Sbjct: 185 RTSYFRVAKSGPSALAIDNDGSSSDEDETMSTCPVITAKTITKPRPIPRQAGFGTFLAAS 244

Query: 244 ANLPFQSKGFSDGF---RGRKL--------------------------------LQIKRG 303
           A+LP Q+K  ++ +     R+L                                L IKRG
Sbjct: 245 ASLPLQAKSLAEKYAHASSRRLLNERIVEHSALGQWLGWLMAAIYMGGRIPQIWLNIKRG 304

Query: 304 SVEGLNPLMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIY 343
           SVEGLNPLMF+FALVANATYV SI+VR+TEWD IK N+PWLLDA+VCVVLDLFII+QYIY
Sbjct: 305 SVEGLNPLMFIFALVANATYVGSILVRTTEWDNIKPNLPWLLDAIVCVVLDLFIILQYIY 364

[Close]

BLAST of Carg20944 vs. TAIR10
Match: AT2G41050.1 (PQ-loop repeat family protein / transmembrane family protein)

HSP 1 Score: 199.9 bits (507), Expect = 2.6e-51
Identity = 137/369 (37.13%), Postives = 196/369 (53.12%), Query Frame = 0


Query: 27  NLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKSSHGVSILFLLTWVAGDAFNLLGCLLE 86
           + RD +S   G++S++ WG+AEIPQI+TN+  KS+ G+SI FL TW+ GD FNLLGCL+E
Sbjct: 6   SFRDGLSLSLGIISVISWGVAEIPQIMTNYSEKSTEGLSITFLTTWMIGDIFNLLGCLME 65

Query: 87  PATLPTQLYTALLYTVNTIVLVLQSVYYDYV---TRSRSDRKTKSHQTAAEEENTPLKG- 146
           PATLPTQ Y ALLYTV T VL +QS+YY ++    ++R D+  ++ + +    +  + G 
Sbjct: 66  PATLPTQFYMALLYTVTTSVLYVQSIYYGHIYPRLKNRRDQMVEAERISNIISDVKIPGR 125

Query: 147 ---SKGVSYAG------IPIPKASPKATPRREFYFTSARSLAGSDTPPFRAFLRL----- 206
              S   +  G        IP +   +   RE ++TSARSL+ S TPP  + L       
Sbjct: 126 WRNSSDTTTCGGQTTPITMIPGSQRTSFTGRELFYTSARSLSSSHTPPAGSVLAQRMARG 185

Query: 207 ---PKSEPSALGNDSSSXXXXXETAAVS---------SNSAFTQPRPIPRSVGYGTFL-- 266
              P  E   L  D +          VS           +  ++ R +    G   F+  
Sbjct: 186 YSEPTLEEPLLPEDVTHPSTKSLLCVVSVFLFLGTFNLPNLLSESRTMALGEGDRVFVVR 245

Query: 267 -------AASANLPFQSKGFSDG------------FRGRKLLQI----KRGSVEGLNPLM 326
                    S+N+   S G S              + G +L QI    +RG VEGLNPLM
Sbjct: 246 AARKLLQVTSSNVAEHSGGESSRIGMFLGWAMAAIYMGGRLPQICLNMRRGHVEGLNPLM 305

Query: 327 FVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYRSFRRKRQ 341
           F FALV N TYVASI+V S EW K+  N+PWL+DA  CVVLD  I++Q+ ++R  R+ + 
Sbjct: 306 FFFALVGNMTYVASILVNSVEWLKLAPNLPWLVDAGGCVVLDFLILLQFFHFRC-RKDKD 365

[Close]

BLAST of Carg20944 vs. TAIR10
Match: AT4G20100.1 (PQ-loop repeat family protein / transmembrane family protein)

HSP 1 Score: 179.9 bits (455), Expect = 2.7e-45
Identity = 111/311 (35.69%), Postives = 169/311 (54.34%), Query Frame = 0


Query: 28  LRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKSSHGVSILFLLTWVAGDAFNLLGCLLEP 87
           +RDD+S   G++S++ W +AEIPQI+TN+  KS  GVSI FL TW+ GD FN++GCL+EP
Sbjct: 2   IRDDLSLSLGIISVISWSVAEIPQIMTNYNQKSIEGVSITFLTTWMLGDIFNVVGCLMEP 61

Query: 88  ATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRSRSDRKTKSHQTAAEEENTPLKGSKGVS 147
           A+LP Q YTA+LYT+ T+VL +QS+YY ++   R  +  ++HQ    EE    + +K  S
Sbjct: 62  ASLPVQFYTAVLYTLATLVLYVQSIYYGHI-YPRLMKNRRNHQMVDVEEPLLREEAKRPS 121

Query: 148 YAGIPIPKASPKATPRREFYFTSARSLAGSDTPPFRAFLRLPKSEPSALGNDSSSXXXXX 207
                    S         +  S   L+GS +   R      K     +G   +      
Sbjct: 122 -------TKSLLCVVSVFLFLGSFNVLSGSRSMDLRG-----KDRVFVVGGAGARKLLEV 181

Query: 208 ETAAVSSNSAFTQPRPIPRSVGYGTFLAASANLPFQSKGFSDGFRGRKLLQIKRGSVEGL 267
            +  +  N+          ++G     A +A        +  G   +  + ++RG+VEGL
Sbjct: 182 SSGNLGENN----------NIGMWLGWAMAAI-------YMGGRLPQICMNVRRGNVEGL 241

Query: 268 NPLMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYRSFR 327
           NPLMF FA + N TYVASI+V S EW KI+ N+PWL+D+  C VLD  I++Q+ Y+   +
Sbjct: 242 NPLMFFFAFIGNVTYVASILVNSVEWSKIEPNLPWLVDSGGCAVLDFLILLQFFYFHCRK 282

Query: 328 RKRQSGGREYE 339
            +  S  +++E
Sbjct: 302 VEADSVKKKHE 282

[Close]

BLAST of Carg20944 vs. Swiss-Prot
Match: sp|Q12010|YPQ1_YEAST (Probable vacuolar amino acid transporter YPQ1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YPQ1 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 3.9e-17
Identity = 89/323 (27.55%), Postives = 135/323 (41.80%), Query Frame = 0


Query: 29  RDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKSSHGVSILFLLTWVAGDAFNLLGCLLEPA 88
           R  +S   G +S+ CW +  +PQI  NF  KSS G+S+LF++ W+AGD FNL+G +++  
Sbjct: 10  RSTLSGISGSISISCWIIVFVPQIYENFYRKSSDGLSLLFVVLWLAGDVFNLMGAVMQ-H 69

Query: 89  TLPTQLYTALLYTVNTIVLVLQSVYYDYVTRSRSDRKTKSHQTAAEE--------ENTPL 148
            L T +  A  YTV  I+L+ Q ++YD   +   D    S      E        E  PL
Sbjct: 70  LLSTMIILAAYYTVADIILLGQCLWYDNEEKPAVDPIHLSPANPINENVLHDVFNEQQPL 129

Query: 149 KGSKGVSYAGIPIPKASPKATPRR---EFYFTSARSLAGSDTPPFRAFLRLPKSEPSALG 208
             S+G                P R   E    S+   AG D         L K      G
Sbjct: 130 LNSQG---------------QPNRIDEEMAAPSSDGNAGDDNLREVNSRNLIKDIFIVSG 189

Query: 209 NDSSSXXXXXETAAVSSNSAFTQPRPI---------------PRSVGY-GTFLAASANLP 268
                      T  V+    +TQP P+                +  GY    L   + +P
Sbjct: 190 VVFVGFISWYVTYCVN----YTQPPPVEDPSLPVPELQINWMAQIFGYLSALLYLGSRIP 249

Query: 269 FQSKGFSDGFRGRKLLQIKRGSVEGLNPLMFVFALVANATYVASIVVRSTEWDKIKANMP 325
                       + LL  KR S EG++ L F+FA + N T++ S++V S +W  +  N  
Sbjct: 250 ------------QILLNFKRKSCEGISFLFFLFACLGNTTFIFSVIVISLDWKYLIMNAS 300

[Close]

BLAST of Carg20944 vs. Swiss-Prot
Match: sp|Q6ZP29|LAAT1_HUMAN (Lysosomal amino acid transporter 1 homolog OS=Homo sapiens OX=9606 GN=PQLC2 PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 2.6e-13
Identity = 72/318 (22.64%), Postives = 130/318 (40.88%), Query Frame = 0


Query: 14  VGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRT-KSSHGVSILFLLTW 73
           + W+     +C  +  D+ S G G++S+LC+  +  PQ +  ++T      +S+ FLL W
Sbjct: 19  IQWIWDVLGECAQDGWDEASVGLGLISILCFAASTFPQFIKAYKTGNMDQALSLWFLLGW 78

Query: 74  VAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRSRSDRKTKSHQTA 133
           + GD+ NL+G  L    LP Q YTA+ Y +  +V++    YY + TR             
Sbjct: 79  IGGDSCNLIGSFLAD-QLPLQTYTAVYYVLADLVMLTLYFYYKFRTRP---------SLL 138

Query: 134 AEEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTPPFRAFLRLPKSE 193
           +   N+ L    G++ A   +  A P A PR  F   +  S+     P            
Sbjct: 139 SAPINSVLLFLMGMACATPLLSAAGPVAAPREAFRGRALLSVESGSKP------------ 198

Query: 194 PSALGNDSSSXXXXXETAAVSSNSAFTQPRPIPRSVG-YGTFLAASANLPFQSKGFSDGF 253
                                    FT+   I   +G   + L   + LP     F    
Sbjct: 199 -------------------------FTRQEVIGFVIGSISSVLYLLSRLPQIRTNF---- 258

Query: 254 RGRKLLQIKRGSVEGLNPLMFVFALVANATYVASIVVRS-----TEWDKIKANMPWLLDA 313
                    R S +G++  +F   ++ N  Y  S+++++     +E   +  ++PWL+ +
Sbjct: 259 --------LRKSTQGISYSLFALVMLGNTLYGLSVLLKNPEEGQSEGSYLLHHLPWLVGS 277

Query: 314 VVCVVLDLFIIVQYIYYR 325
           +  ++LD  I +Q++ YR
Sbjct: 319 LGVLLLDTIISIQFLVYR 277

[Close]

BLAST of Carg20944 vs. Swiss-Prot
Match: sp|Q95XZ6|LAAT1_CAEEL (Lysosomal amino acid transporter 1 OS=Caenorhabditis elegans OX=6239 GN=laat-1 PE=1 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 3.4e-13
Identity = 76/313 (24.28%), Postives = 124/313 (39.62%), Query Frame = 0


Query: 14  VGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKSSHGVSILFLLTWV 73
           + W++  F DC+      + F  G++SL  W +   PQ+  N++TK   G+S+ FL  W+
Sbjct: 23  IQWIKDVFTDCVDTDLKLLGFIIGLISLALWLIPLFPQLWQNYKTKKCEGLSLAFLFFWL 82

Query: 74  AGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRSRSDRKTKSHQTAA 133
            GD  N+LG +L     P Q    + Y +  +VL  Q  YY  +    +    +S+    
Sbjct: 83  VGDTCNMLGAILTNQQ-PIQKIIGVYYIIQDLVLWTQYGYYLKIYNRPTTSSARSNTIVV 142

Query: 134 EEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTPPFRAFLRLPKSEP 193
                   GS  V  + +P P    +   +R F      SL   +  P    L++     
Sbjct: 143 PVLALASVGSFFVFESALP-PVGDHRV--KRSF----LESLNHQEGLPLEGILKM----- 202

Query: 194 SALGNDSSSXXXXXETAAVSSNSAFTQPRPIPRSVGY--GTFLAASANLPFQSKGFSDGF 253
                                   FT    +   +GY  G+  A           +  G 
Sbjct: 203 ---------------------WPIFTSYTDM---LGYIIGSMAAVC---------YFGGR 262

Query: 254 RGRKLLQIKRGSVEGLNPLMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVV 313
             + +   +  S EGL+  MF   + AN TY  S+++ +T W  +  ++PWL  ++ C  
Sbjct: 263 IPQIIKNYRHSSCEGLSLTMFYIIVAANFTYGISVLLATTSWLYLLRHLPWLAGSLGCCC 289

Query: 314 LDLFIIVQYIYYR 325
            D  II QY  YR
Sbjct: 323 FDAVIISQYYLYR 289

[Close]

BLAST of Carg20944 vs. Swiss-Prot
Match: sp|P38279|YPQ3_YEAST (Probable vacuolar amino acid transporter YPQ3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RTC2 PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 2.9e-12
Identity = 67/292 (22.95%), Postives = 128/292 (43.84%), Query Frame = 0


Query: 37  GVVSLLCWGLAEIPQIVTNFRTKSSHGVSILFLLTWVAGDAFNLLGCLLEPATLPTQLYT 96
           G +S+ CW +  +PQI  NFR +S+ G+S+LF++ W+ GD FN++G +++   LPT +  
Sbjct: 18  GSISICCWIVVFVPQIYENFRRQSAEGLSLLFIVLWLLGDIFNVMGAMMQ-NLLPTMIIL 77

Query: 97  ALLYTVNTIVLVLQSVYYDYVTRSRSDRKTKS----HQTAAEEENTPLKGSKGVSYAGIP 156
           A  YT+  ++L++Q ++YD   +S      K+    H   A   N  +       Y  + 
Sbjct: 78  AAYYTLADLILLIQCMWYDKEKKSILQEVKKNVDPVHLPPANPINETVLQDVFNEYEPL- 137

Query: 157 IPKASPKATPRREFYFTSARSLAGSDTPPFRAFLRLPKSEPSALGNDSSSXXXXXETAAV 216
           +P+   + +            +       F  FL +     + + +   S     +    
Sbjct: 138 LPRIEEEDSQSYSSLELGRTIVVKERENFFNDFLIVSGVLIAGILSWYISYCSGLDNGIP 197

Query: 217 SSNSAFTQPRPIPRSVGY-GTFLAASANLPFQSKGFSDGFRGRKLLQIKRGSVEGLNPLM 276
               AF Q     + +GY    L   + +P            + +L  KR S EG++ L 
Sbjct: 198 KKKPAFEQINLPAQILGYLSAILYLGSRIP------------QIVLNFKRKSCEGVSFLF 257

Query: 277 FVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYY 324
           F+FA + N +++ S++  S           WL+ +   +++D  + +Q+  Y
Sbjct: 258 FLFACLGNTSFIISVLSAS-----------WLIGSAGTLLMDFTVFIQFFLY 284

[Close]

BLAST of Carg20944 vs. Swiss-Prot
Match: sp|Q8C4N4|LAAT1_MOUSE (Lysosomal amino acid transporter 1 homolog OS=Mus musculus OX=10090 GN=Pqlc2 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.9e-11
Identity = 77/322 (23.91%), Postives = 136/322 (42.24%), Query Frame = 0


Query: 14  VGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRT-KSSHGVSILFLLTW 73
           V W+   F +C  +  D+ S G G+VS+LC+  +  PQ +   +T      +S+ FLL W
Sbjct: 19  VQWIWDVFGECAQDGWDEASVGLGLVSILCFAASTFPQYIKACKTGNMDQALSLWFLLGW 78

Query: 74  VAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRSRSDRKTKSHQTA 133
           + GD+ NL+G  L    LP Q YTA+ Y +  ++++  ++Y+ Y  + R           
Sbjct: 79  IGGDSCNLIGSFLAD-QLPLQTYTAVYYVLADLMML--TLYFHYKFKKRP-------SPL 138

Query: 134 AEEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTPPFRAFLRLPKSE 193
           +   N+ L    G       +    P A PR  F   +  S+                 E
Sbjct: 139 SAPINSVLLFILGTVCITPLLSSTDPVAVPREGFRGRTLLSV-----------------E 198

Query: 194 PSALGNDSSSXXXXXETAAVSSNSAFTQPRPIPRSVGYGTFLAASANLPFQSKGFSDGFR 253
           P                     N  FT+   I   +G     +AS+ L   S        
Sbjct: 199 P--------------------GNKPFTKKEVIGFVIG-----SASSLLYLLS-------- 258

Query: 254 GRKLLQIK----RGSVEGLNPLMFVFALVANATYVASIVVRS-----TEWDKIKANMPWL 313
             +L QI+    R S +G++  +F   ++ N  Y  S+++++     +E   +  ++PWL
Sbjct: 259 --RLPQIRTNFIRQSTQGISYSLFALVMLGNTLYGLSVLLKNPEVGQSEGSYLLHHLPWL 278

Query: 314 LDAVVCVVLDLFIIVQYIYYRS 326
           + ++  ++LD  I +Q++ YRS
Sbjct: 319 VGSLGVLLLDTIISIQFLVYRS 278

[Close]

BLAST of Carg20944 vs. TrEMBL
Match: tr|A0A1S3C6I6|A0A1S3C6I6_CUCME (probable vacuolar amino acid transporter YPQ1 OS=Cucumis melo OX=3656 GN=LOC103497459 PE=4 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 9.7e-154
Identity = 293/379 (77.31%), Postives = 307/379 (81.00%), Query Frame = 0


Query: 1   MKGGYCVKEQKACVGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKS 60
           M+G YCVKEQK CVGWVETYFKDCLCNLRDDMSFGFG+VSLLCWGLAEIPQIVTNFRT  
Sbjct: 1   MEGWYCVKEQKRCVGWVETYFKDCLCNLRDDMSFGFGLVSLLCWGLAEIPQIVTNFRTXX 60

Query: 61  SHGVSILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRS 120
                       VAGD FNL+GCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVT+ 
Sbjct: 61  XXXXXXXXXXXXVAGDVFNLVGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTKW 120

Query: 121 RSDRKTKSHQTAAEEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTP 180
             DRK+KS QT  EEE TPLKG+KGV YAGIPIPKASPK TPRREFY+TSARSLAGSDTP
Sbjct: 121 CIDRKSKSDQT-VEEEKTPLKGNKGVGYAGIPIPKASPKPTPRREFYYTSARSLAGSDTP 180

Query: 181 PFRAFLRLPKSEPSALGNDSSSXXXXXETAAVSSNSAFTQPRPIPRSVGYGTFLAASANL 240
           PFRAFLRLPKS PSALGNDSSS     +TAAV S+SA TQPRPIPRSVGYGTFLAASANL
Sbjct: 181 PFRAFLRLPKSGPSALGNDSSSSDEESDTAAVFSHSAVTQPRPIPRSVGYGTFLAASANL 240

Query: 241 PFQSKGFSDGFRGRKLLQ-------------------------------IKRGSVEGLNP 300
           PFQSKGFSDGF GRKLLQ                               IKRGSVEGLNP
Sbjct: 241 PFQSKGFSDGFSGRKLLQEHSSHSGFGQLLGWLMAAIYMGGRLPQIWLNIKRGSVEGLNP 300

Query: 301 LMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYRSFRRK 345
           LMFVFAL+ANATYVASIVVRSTEW+ IKANMPWLLDAVVCV+LDLFII+QYIYYR FRRK
Sbjct: 301 LMFVFALIANATYVASIVVRSTEWENIKANMPWLLDAVVCVLLDLFIILQYIYYRRFRRK 360

[Close]

BLAST of Carg20944 vs. TrEMBL
Match: tr|A0A0A0LMT2|A0A0A0LMT2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G295430 PE=4 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 8.2e-153
Identity = 290/382 (75.92%), Postives = 306/382 (80.10%), Query Frame = 0


Query: 1   MKGGYCVKEQKACVGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKS 60
           M+G YCVKEQK CVGWVETYFKDCLCNLRDDMSFGFG+VSLLCWGLAEIPQIVTNFRT  
Sbjct: 1   MEGWYCVKEQKRCVGWVETYFKDCLCNLRDDMSFGFGLVSLLCWGLAEIPQIVTNFRTXX 60

Query: 61  SHGVSILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRS 120
                       VAGD FNL+GCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVT+ 
Sbjct: 61  XXXXXXXXXXXXVAGDVFNLVGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTKC 120

Query: 121 RSDRKTKSHQTAAEEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTP 180
             DRK KS  T  EEE TPLKG+KGV Y GIPIPKASPK TPRREFY+TSARSLAGSDTP
Sbjct: 121 CIDRKAKSDHT-GEEEKTPLKGNKGVGYVGIPIPKASPKPTPRREFYYTSARSLAGSDTP 180

Query: 181 PFRAFLRLPKSEPSALGNDSSSXXXXXETAAVSSNSAFTQPRPIPRSVGYGTFLAASANL 240
           PFRAFLRLPKS PSALGNDSSS     +TAAV S+SA TQPRPIPRSVGYGTFLAASANL
Sbjct: 181 PFRAFLRLPKSGPSALGNDSSSSDDESDTAAVFSHSAVTQPRPIPRSVGYGTFLAASANL 240

Query: 241 PFQSKGFSDGFRGRKLLQ-------------------------------IKRGSVEGLNP 300
           PFQ+KGFSDGF GRKLLQ                               IKRGSVEGLNP
Sbjct: 241 PFQTKGFSDGFSGRKLLQEHSSHSGFGQLLGWLMAAIYMGGRLPQIWLNIKRGSVEGLNP 300

Query: 301 LMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYRSFRRK 347
           LMFVFAL+ANATYVASIVVRSTEW+ IKANMPWLLDAVVCV+LDLFII+QYIYYR FRR+
Sbjct: 301 LMFVFALIANATYVASIVVRSTEWESIKANMPWLLDAVVCVLLDLFIILQYIYYRRFRRQ 360

[Close]

BLAST of Carg20944 vs. TrEMBL
Match: tr|A0A2I4F4D8|A0A2I4F4D8_9ROSI (probable vacuolar amino acid transporter YPQ1 OS=Juglans regia OX=51240 GN=LOC108995386 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 2.4e-120
Identity = 234/373 (62.73%), Postives = 267/373 (71.58%), Query Frame = 0


Query: 5   YCVKEQKACVGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKSSHGV 64
           YC KEQK C  WVE YFKDCLCNLRD++SFGFG +SL+CWG+AEIPQI+TNF+TKS HGV
Sbjct: 3   YCAKEQKPCARWVEKYFKDCLCNLRDELSFGFGFISLVCWGVAEIPQIITNFQTKSGHGV 62

Query: 65  SILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRSRSDR 124
           S+ FLLTW+ GD FNL+GCLLEPATLPTQ YTALLYTV+TIVLVLQSVYYDY+ R    R
Sbjct: 63  SLAFLLTWIVGDIFNLVGCLLEPATLPTQFYTALLYTVSTIVLVLQSVYYDYIRRWFKRR 122

Query: 125 KTKSHQTAAEEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTPPFRA 184
           + K++  A E+   PL   K V   GIPIP+ASPKATPRREFY TSARSLAGS TPPFR 
Sbjct: 123 QVKANHPAEEDHKKPLMRPKSVD-PGIPIPRASPKATPRREFYITSARSLAGSGTPPFRT 182

Query: 185 FLRLPKSEPSALGNDSSSXXXXXETAAVSSNSAFTQPRPIPRSVGYGTFLAASANLPFQS 244
           +L   KS PSA+G +S S     E A   S  + +QPRPIPRSVGYGTFLA S NLP  S
Sbjct: 183 YLTAAKSGPSAMGLNSDS-SSDDEAAPAHSKKSTSQPRPIPRSVGYGTFLATSLNLPLHS 242

Query: 245 KGFSD---GFRGRKL--------------------------------LQIKRGSVEGLNP 304
           KG ++   G  GRKL                                L IKRGSVEGLNP
Sbjct: 243 KGLTEAYLGLTGRKLLHEQGGVESVYGQWLGWLMAAIYMGGRLPQIWLNIKRGSVEGLNP 302

Query: 305 LMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYRSFRR- 340
           LMF+FALVAN TYVASI+VR+TEW+KIKANMPWLLDAVVCV LDLFII+QYIYY+  R+ 
Sbjct: 303 LMFIFALVANVTYVASILVRTTEWEKIKANMPWLLDAVVCVALDLFIILQYIYYKHLRKT 362

[Close]

BLAST of Carg20944 vs. TrEMBL
Match: tr|A0A2C9UV83|A0A2C9UV83_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_12G099200 PE=4 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 6.5e-118
Identity = 230/373 (61.66%), Postives = 264/373 (70.78%), Query Frame = 0


Query: 1   MKGGYCVKEQKACVGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKS 60
           M   YCV EQK CVGWVE YFKDCLCNLRDD SFGFG++SL+CWG+AEIPQI+TNFRTKS
Sbjct: 1   MSLAYCVTEQKPCVGWVEKYFKDCLCNLRDDFSFGFGLISLVCWGVAEIPQIITNFRTKS 60

Query: 61  SHGVSILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRS 120
           SHGVS+LFLLTWVAGD FNL+GCLLEPATLPTQ YTALLYTV+T+VLVLQ  YYD++ + 
Sbjct: 61  SHGVSLLFLLTWVAGDVFNLVGCLLEPATLPTQFYTALLYTVSTVVLVLQGFYYDHIYKL 120

Query: 121 RSDRKTKSHQTAAEEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTP 180
              ++  + Q A +++  PLK     S   IPIP ASP+ATPRRE+Y+TSARS+A S TP
Sbjct: 121 WKWQRADADQQAVDDDKKPLKPKSAGS--AIPIPTASPRATPRREYYYTSARSMASSGTP 180

Query: 181 PFRAFLRLPKSEPSALGNDSSSXXXXXETAAVSSNSAFTQPRPIPRSVGYGTFLAASANL 240
           PFR++LR  +S PSA+G D  S     E A+V S    +QPRPIPRS GYGTFLA S NL
Sbjct: 181 PFRSYLRAARSGPSAMGLDHES-SSDDEAASVPSKFV-SQPRPIPRSAGYGTFLATSLNL 240

Query: 241 PFQSKGFSD---GFRGRKL---------------------------------LQIKRGSV 300
           P  SK  +D   GF GR+L                                 L IKRGSV
Sbjct: 241 PLHSKALTDAYIGFTGRRLLHEGGAMEHSSFGQWLGWLMAAIYMGGRIPQIWLNIKRGSV 300

Query: 301 EGLNPLMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYR 338
           EGLNPLMF+FALVAN TYV SI+VR+TEWD IKANMPWLLDA VCV LDLFII+QYIYYR
Sbjct: 301 EGLNPLMFIFALVANLTYVLSILVRTTEWDSIKANMPWLLDAAVCVALDLFIILQYIYYR 360

[Close]

BLAST of Carg20944 vs. TrEMBL
Match: tr|B9T4V4|B9T4V4_RICCO (Uncharacterized protein OS=Ricinus communis OX=3988 GN=RCOM_0411610 PE=4 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 4.2e-117
Identity = 229/373 (61.39%), Postives = 265/373 (71.05%), Query Frame = 0


Query: 1   MKGGYCVKEQKACVGWVETYFKDCLCNLRDDMSFGFGVVSLLCWGLAEIPQIVTNFRTKS 60
           M   YCVKEQK CV WVE YFKDCLC+L+D++SFGFG VSL+ WG+AE+PQI+TNFRTKS
Sbjct: 1   MSLAYCVKEQKPCVRWVERYFKDCLCSLKDELSFGFGFVSLVSWGVAEVPQIITNFRTKS 60

Query: 61  SHGVSILFLLTWVAGDAFNLLGCLLEPATLPTQLYTALLYTVNTIVLVLQSVYYDYVTRS 120
           SHGVS+LFLLTWVAGD FNL+GCLLEPATLPTQ YTALLYT +TIVLVLQ +YYDY+ R 
Sbjct: 61  SHGVSLLFLLTWVAGDVFNLVGCLLEPATLPTQFYTALLYTTSTIVLVLQGLYYDYIYRW 120

Query: 121 RSDRKTKSHQTAAEEENTPLKGSKGVSYAGIPIPKASPKATPRREFYFTSARSLAGSDTP 180
              +K + +Q   E+E  PLK   G S  GIPIP AS ++TPRRE+Y+TSARS+A S TP
Sbjct: 121 WKGQKNEVNQ-QVEDEKKPLKPKLGDS--GIPIPNASTRSTPRREYYYTSARSMASSGTP 180

Query: 181 PFRAFLRLPKSEPSALGNDSSSXXXXXETAAVSSNSAFTQPRPIPRSVGYGTFLAASANL 240
           PFR +LR  KS PSA+G D  S     E A VS+ S  +QPRPIPRS GYGTFLA S NL
Sbjct: 181 PFRGYLRTAKSGPSAMGFDHESSSSDDEAAPVSAASV-SQPRPIPRSAGYGTFLATSLNL 240

Query: 241 PFQSKGFSDGFRG---RKL---------------------------------LQIKRGSV 300
           P QSK  +D + G   R+L                                 L IKRGSV
Sbjct: 241 PLQSKALTDAYIGVTSRRLLHEGSGMEHSAFGQWLGWLMAAIYMGGRIPQIWLNIKRGSV 300

Query: 301 EGLNPLMFVFALVANATYVASIVVRSTEWDKIKANMPWLLDAVVCVVLDLFIIVQYIYYR 338
           EGLNPLMF+FALVAN TYV SIVVR+TEW+ IKANMPWLLDA VCV LD FII+QY+YYR
Sbjct: 301 EGLNPLMFIFALVANLTYVLSIVVRTTEWESIKANMPWLLDAAVCVALDFFIILQYVYYR 360

The following BLAST results are available for this feature:

BLAST of Carg20944 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022927971.1	1.2e-184	90.98	probable vacuolar amino acid transporter YPQ3 [Cucurbita moschata]	[more]
XP_023512444.1	1.3e-181	89.92	probable vacuolar amino acid transporter YPQ3 [Cucurbita pepo subsp. pepo]	[more]
XP_022971727.1	6.5e-178	88.33	probable vacuolar amino acid transporter YPQ3 [Cucurbita maxima]	[more]
XP_022155474.1	1.3e-154	79.42	probable vacuolar amino acid transporter YPQ1 [Momordica charantia]	[more]
XP_008457880.1	1.5e-153	77.31	PREDICTED: probable vacuolar amino acid transporter YPQ1 [Cucumis melo] >XP_0084...	[more]

BLAST of Carg20944 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G36850.1	2.1e-109	56.36	PQ-loop repeat family protein / transmembrane family protein	[more]
AT2G41050.1	2.6e-51	37.13	PQ-loop repeat family protein / transmembrane family protein	[more]
AT4G20100.1	2.7e-45	35.69	PQ-loop repeat family protein / transmembrane family protein	[more]

BLAST of Carg20944 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q12010\|YPQ1_YEAST	3.9e-17	27.55	Probable vacuolar amino acid transporter YPQ1 OS=Saccharomyces cerevisiae (strai...	[more]
sp\|Q6ZP29\|LAAT1_HUMAN	2.6e-13	22.64	Lysosomal amino acid transporter 1 homolog OS=Homo sapiens OX=9606 GN=PQLC2 PE=1...	[more]
sp\|Q95XZ6\|LAAT1_CAEEL	3.4e-13	24.28	Lysosomal amino acid transporter 1 OS=Caenorhabditis elegans OX=6239 GN=laat-1 P...	[more]
sp\|P38279\|YPQ3_YEAST	2.9e-12	22.95	Probable vacuolar amino acid transporter YPQ3 OS=Saccharomyces cerevisiae (strai...	[more]
sp\|Q8C4N4\|LAAT1_MOUSE	1.9e-11	23.91	Lysosomal amino acid transporter 1 homolog OS=Mus musculus OX=10090 GN=Pqlc2 PE=...	[more]

BLAST of Carg20944 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3C6I6\|A0A1S3C6I6_CUCME	9.7e-154	77.31	probable vacuolar amino acid transporter YPQ1 OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
tr\|A0A0A0LMT2\|A0A0A0LMT2_CUCSA	8.2e-153	75.92	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G295430 PE=4 SV=1	[more]
tr\|A0A2I4F4D8\|A0A2I4F4D8_9ROSI	2.4e-120	62.73	probable vacuolar amino acid transporter YPQ1 OS=Juglans regia OX=51240 GN=LOC10...	[more]
tr\|A0A2C9UV83\|A0A2C9UV83_MANES	6.5e-118	61.66	Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_12G099200 PE=4 SV=...	[more]
tr\|B9T4V4\|B9T4V4_RICCO	4.2e-117	61.39	Uncharacterized protein OS=Ricinus communis OX=3988 GN=RCOM_0411610 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006603	PQ-loop_rpt

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg20944-RA	Carg20944-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006603	PQ-loop repeat	SMART	SM00679	ctns	coord: 253..280 e-value: 0.31 score: 18.5 coord: 46..77 e-value: 1.9E-9 score: 47.4
IPR006603	PQ-loop repeat	PFAM	PF04193	PQ-loop	coord: 33..87 e-value: 1.5E-17 score: 63.0 coord: 259..293 e-value: 7.5E-6 score: 25.5
None	No IPR available	GENE3D	G3DSA:1.20.1280.290		coord: 29..123 e-value: 1.0E-7 score: 33.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 194..220
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 193..220
None	No IPR available	PANTHER	PTHR16201:SF43	PQ-LOOP REPEAT FAMILY PROTEIN / TRANSMEMBRANE FAMILY PROTEIN	coord: 257..344 coord: 3..258
None	No IPR available	PANTHER	PTHR16201	FAMILY NOT NAMED	coord: 257..344 coord: 3..258

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg20944	Cla020135	Watermelon (97103) v1	carwmB0993
Carg20944	Csa2G295430	Cucumber (Chinese Long) v2	carcuB1006
Carg20944	MELO3C020942	Melon (DHL92) v3.5.1	carmeB0967
Carg20944	ClCG02G013280	Watermelon (Charleston Gray)	carwcgB0967
Carg20944	CSPI02G14910	Wild cucumber (PI 183967)	carcpiB1030
Carg20944	Cucsa.250590	Cucumber (Gy14) v1	carcgyB1109
Carg20944	CmaCh20G011250	Cucurbita maxima (Rimu)	carcmaB1374
Carg20944	CmoCh20G011580	Cucurbita moschata (Rifu)	carcmoB1331
Carg20944	Lsi10G006430	Bottle gourd (USVL1VR-Ls)	carlsiB906
Carg20944	Cp4.1LG16g00310	Cucurbita pepo (Zucchini)	carcpeB1378
Carg20944	CsGy2G014910	Cucumber (Gy14) v2	carcgybB0979
Carg20944	MELO3C020942.2	Melon (DHL92) v3.6.1	carmedB0977
Carg20944	CsaV3_2G017460	Cucumber (Chinese Long) v3	carcucB1036
Carg20944	Cla97C02G038970	Watermelon (97103) v2	carwmbB1045
Carg20944	Bhi10G001839	Wax gourd	carwgoB1163

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg20944	Cucurbita maxima (Rimu)	carcmaB1371