Cp4.1LG18g00200 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG18g00200
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionBeta-1,3-glucanase, putative
LocationCp4.1LG18 : 376922 .. 378601 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAATGACAACCACAAAAATGGTTAAAATTTCCCACATGATTGGACTCTTCATGGCTGCCATGTCTTTAGTCGTTGGAGCATACGATGTTCTTCTTGGCGCCTACTACGGCCTTTTAGGTGACAACTTACCATCCCCATCGTCAGTGGTGCAACTCTGTCGAAAATACAACATTTCCCGCGTTCGATTGGAAGTTCCAAACCTCGATGTTATAGAAGGGTTTCGTGGCACACAAATCGACTTATCTTTCGGTGTCCCCAACGACATGTTACCAAACATGGCGAGCAATAAGACTTTGGTTGAGTAGTGGTTTAACACCTATGTCGAGCCCTACACTAACGATTGCACTATCAATTATATCGTTGTCGGTGACAAGGCCATCCCCGGCCTCGACGACAACATATTGCCTGTCATGAAATCCCTTCAAGATCTCCTCAACGCTCGCTACTTCGGAGCAGTGAAACTCACCACATTGGTTGGTTTCAACGCATTGGCCGTTACAAACCCTCCATCCAGCGGCGCATTCAACCCGGTTATTGTTGAGAACATGAGAGGAATCCTCGAGTTCTTACGAGGACAAGGGTCTCCTCTTATGGTTAGCGTATATACGTATGATCAATATGTTTATGGTGGTAATATAAGCTTGGGTTATGCGACGCTTAGTGAACAAAACACGTTGGTTCGCGATGGGATGTTGAGTTATAATAACTTGTTTGATGAAATGGTGGATGCGTTTTATGCAGCCATGGATAAGGAAAATGTTGGAGATGTGGCCATTGTTTTTGGGGAAACTGGTTGGCCTACTTGTGGAAATTATAACGCTACGACACCGTTACTTGCAGCGGAGTTCAATCGGAACTTCAAGAGTCATATTAGCTCAGGAAAGGGGACACCGAAAAAGGAAAATATTTATATTGAAGGATTTATTAGGAGTATATTCAATGAGAATCAAAAGCCCGAAGGAGAGTCACGGTGTTATGGCATGTTCAACGTTGATTCCACACCTATTTACTCCGTGTTTTAACTGGGTTAGATGTATCCTATGGTAAAATGGTGTTTTTCATGGGAAGTTTTTGGTACTAAATACTAAGATAGCTTGAAAAAATAACTTAGCATATTACTTCTATTCCTTCCAATGTGTGTGAGCTTGTTTGTCAAATAAAAATATTATTAGTTAGAGTATGTGCGGATTGGGTTAGGTCAACCCAACCCAAAAAAGTTAATGTTCGGTTGGGTTGATATCCGTTGAATTAAATAATGAATCAAATCCAACTCAAGCATAAATTTTGTATGTNTAAGCTTGGGTTATGCGACGCTTAGTGAACAAAACACGTTGGTTCGCGATGGGATGTTGAGTTATAATAACTTGTTTGATGAAATGGTGGATGCGTTTTATGCAGCCATGGATAAGGAAAATGTTGGAGATGTGGCCATTGTTTTTGGGGAAACTGGTTGGCCTACTTGTGGAAATTATAACGCTACGACACCGTTACTTGCAGCGGAGTTCAATCGGAACTTCAAGAGTCATATTAGCTCAGGAAAGGGGACACCGAAAAAGGAAAATATTTATATTGAAGGATTTATTAGGAGTATATTCAATGAGAATCAAAAGCCCGAAGGAGAGTCACGGTGTTATGGCATGTTCAACGTTGATTCCACACCTATTTACTCCGTGTTTTAA

mRNA sequence

ATAATGACAACCACAAAAATGGTTAAAATTTCCCACATGATTGGACTCTTCATGGCTGCCATGTCTTTAGTCGTTGGAGCATACGATGTTCTTCTTGGCGCCTACTACGGCCTTTTAGGTGACAACTTACCATCCCCATCGTCAGTGGTGCAACTCTGTCGAAAATACAACATTTCCCGCGTTCGATTGGAAGTTCCAAACCTCGATGTTATAGAAGGGTTTCGTGGCACACAAATCGACTTATCTTTCGGTGTCCCCAACGACATGTTACCAAACATGGCGAGCAATAAGACTTTGTGGTTTAACACCTATGTCGAGCCCTACACTAACGATTGCACTATCAATTATATCGTTGTCGGTGACAAGGCCATCCCCGGCCTCGACGACAACATATTGCCTGTCATGAAATCCCTTCAAGATCTCCTCAACGCTCGCTACTTCGGAGCAGTGAAACTCACCACATTGGTTGGTTTCAACGCATTGGCCGTTACAAACCCTCCATCCAGCGGCGCATTCAACCCGGTTATTGTTGAGAACATGAGAGGAATCCTCGAGTTCTTACGAGGACAAGGGTCTCCTCTTATGGTTAGCGTATATACGTATGATCAATATGTTTATGGTGGTAATATAAGCTTGGGTTATGCGACGCTTAGTGAACAAAACACGTTGGTTCGCGATGGGATGTTGAGTTATAATAACTTGTTTGATGAAATGGTGGATGCGTTTTATGCAGCCATGGATAAGGAAAATGTTGGAGATGTGGCCATTGTTTTTGGGGAAACTGGTTGGCCTACTTGTGGAAATTATAACGCTACGACACCGTTACTTGCAGCGGAGTTCAATCGGAACTTCAAGAGTCATATTAGCTCAGGAAAGGGGACACCGAAAAAGGAAAATATTTATATTGAAGGATTTATTAGGAGTATATTCAATGAGAATCAAAAGCCCGAAGGAGAGTCACGGTGTTATGGCATGTTCAACGTTGATTCCACACCTATTTACTCCGTTGAACAAAACACGTTGGTTCGCGATGGGATGTTGAGTTATAATAACTTGTTTGATGAAATGGTGGATGCGTTTTATGCAGCCATGGATAAGGAAAATGTTGGAGATGTGGCCATTGTTTTTGGGGAAACTGGTTGGCCTACTTGTGGAAATTATAACGCTACGACACCGTTACTTGCAGCGGAGTTCAATCGGAACTTCAAGAGTCATATTAGCTCAGGAAAGGGGACACCGAAAAAGGAAAATATTTATATTGAAGGATTTATTAGGAGTATATTCAATGAGAATCAAAAGCCCGAAGGAGAGTCACGGTGTTATGGCATGTTCAACGTTGATTCCACACCTATTTACTCCGTGTTTTAA

Coding sequence (CDS)

ATAATGACAACCACAAAAATGGTTAAAATTTCCCACATGATTGGACTCTTCATGGCTGCCATGTCTTTAGTCGTTGGAGCATACGATGTTCTTCTTGGCGCCTACTACGGCCTTTTAGGTGACAACTTACCATCCCCATCGTCAGTGGTGCAACTCTGTCGAAAATACAACATTTCCCGCGTTCGATTGGAAGTTCCAAACCTCGATGTTATAGAAGGGTTTCGTGGCACACAAATCGACTTATCTTTCGGTGTCCCCAACGACATGTTACCAAACATGGCGAGCAATAAGACTTTGTGGTTTAACACCTATGTCGAGCCCTACACTAACGATTGCACTATCAATTATATCGTTGTCGGTGACAAGGCCATCCCCGGCCTCGACGACAACATATTGCCTGTCATGAAATCCCTTCAAGATCTCCTCAACGCTCGCTACTTCGGAGCAGTGAAACTCACCACATTGGTTGGTTTCAACGCATTGGCCGTTACAAACCCTCCATCCAGCGGCGCATTCAACCCGGTTATTGTTGAGAACATGAGAGGAATCCTCGAGTTCTTACGAGGACAAGGGTCTCCTCTTATGGTTAGCGTATATACGTATGATCAATATGTTTATGGTGGTAATATAAGCTTGGGTTATGCGACGCTTAGTGAACAAAACACGTTGGTTCGCGATGGGATGTTGAGTTATAATAACTTGTTTGATGAAATGGTGGATGCGTTTTATGCAGCCATGGATAAGGAAAATGTTGGAGATGTGGCCATTGTTTTTGGGGAAACTGGTTGGCCTACTTGTGGAAATTATAACGCTACGACACCGTTACTTGCAGCGGAGTTCAATCGGAACTTCAAGAGTCATATTAGCTCAGGAAAGGGGACACCGAAAAAGGAAAATATTTATATTGAAGGATTTATTAGGAGTATATTCAATGAGAATCAAAAGCCCGAAGGAGAGTCACGGTGTTATGGCATGTTCAACGTTGATTCCACACCTATTTACTCCGTTGAACAAAACACGTTGGTTCGCGATGGGATGTTGAGTTATAATAACTTGTTTGATGAAATGGTGGATGCGTTTTATGCAGCCATGGATAAGGAAAATGTTGGAGATGTGGCCATTGTTTTTGGGGAAACTGGTTGGCCTACTTGTGGAAATTATAACGCTACGACACCGTTACTTGCAGCGGAGTTCAATCGGAACTTCAAGAGTCATATTAGCTCAGGAAAGGGGACACCGAAAAAGGAAAATATTTATATTGAAGGATTTATTAGGAGTATATTCAATGAGAATCAAAAGCCCGAAGGAGAGTCACGGTGTTATGGCATGTTCAACGTTGATTCCACACCTATTTACTCCGTGTTTTAA

Protein sequence

IMTTTKMVKISHMIGLFMAAMSLVVGAYDVLLGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNLDVIEGFRGTQIDLSFGVPNDMLPNMASNKTLWFNTYVEPYTNDCTINYIVVGDKAIPGLDDNILPVMKSLQDLLNARYFGAVKLTTLVGFNALAVTNPPSSGAFNPVIVENMRGILEFLRGQGSPLMVSVYTYDQYVYGGNISLGYATLSEQNTLVRDGMLSYNNLFDEMVDAFYAAMDKENVGDVAIVFGETGWPTCGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIYIEGFIRSIFNENQKPEGESRCYGMFNVDSTPIYSVEQNTLVRDGMLSYNNLFDEMVDAFYAAMDKENVGDVAIVFGETGWPTCGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIYIEGFIRSIFNENQKPEGESRCYGMFNVDSTPIYSVF
BLAST of Cp4.1LG18g00200 vs. Swiss-Prot
Match: BG4_ARATH (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana GN=BG4 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.5e-64
Identity = 129/312 (41.35%), Postives = 198/312 (63.46%), Query Frame = 1

Query: 32  LGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNLDVIEGFRGTQ-IDLSFGVPNDML 91
           +G  YGLLGDNLPSPS+V+ L +   ISR+R+  PN +V+   RG + I+++ GV +  L
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 92  PNMASNKTL---WFNTYVEPYTNDCTINYIVVGDKAIPG-LDDNILPVMKSLQDLLNARY 151
             +A+++     WF   +E Y  D  I +I VG++ IPG +   +LPVM+SL +L+ +R 
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 153

Query: 152 FGAVKLTTLVGFNALAVTNPPSSGAFNPVIVENMRGILEFLRGQGSPLMVSVYTYDQYVY 211
              + ++T+V  + L  + PPS+G F     E +  +L+ L    +P++V++Y Y  Y  
Sbjct: 154 L-PISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYFAYAS 213

Query: 212 G-GNISLGYATLSEQNTLVRDGMLSYNNLFDEMVDAFYAAMDKENVGDVAIVFGETGWPT 271
              NI L YA+ + ++ +V+DG L Y+N+FD + DAF  AM+KE V ++ +V  ETGWP+
Sbjct: 214 DPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGWPS 273

Query: 272 CGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIYIEGFIRSIFNENQKPEGESRCYGM 331
            GN N TTP +A+ +NRNF  HI+SGKGTPK+ N  + GF+ + FNENQKP G  + +G+
Sbjct: 274 AGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQNFGL 333

Query: 332 FN-VDSTPIYSV 337
           +N  D  PIY +
Sbjct: 334 YNPSDMKPIYKL 344

BLAST of Cp4.1LG18g00200 vs. Swiss-Prot
Match: BG5_ARATH (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana GN=BG5 PE=2 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 4.3e-64
Identity = 137/336 (40.77%), Postives = 206/336 (61.31%), Query Frame = 1

Query: 8   VKISHMIGLFMAAMSLVVGAYDVLLGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPN 67
           V  ++ IG   AA S+         G  YGLLGDNLPSPS V+ L +  +I+++R+  PN
Sbjct: 28  VNAANSIGFVNAANSI---------GLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPN 87

Query: 68  LDVIEGFRGTQ-IDLSFGVPNDMLPNMASNKTL---WFNTYVEPYTNDCTINYIVVGDKA 127
            +V+   RG + I ++ GV +  L  +++++     WF T +EPY +D  I +I VG++ 
Sbjct: 88  TEVLNALRGHRDIAVTVGVRDQDLAALSASEEAVKGWFATNIEPYLSDINIAFITVGNEV 147

Query: 128 IPG-LDDNILPVMKSLQDLLNARYFGAVKLTTLVGFNALAVTNPPSSGAFNPVIVENMRG 187
           IPG +   +LPVM+SL +L+ +R    + ++T+V    L  + PPS+G F     E +  
Sbjct: 148 IPGPIGPQVLPVMQSLTNLVKSRNL-PISISTVVAMWNLEQSYPPSAGMFTSQAREQLVP 207

Query: 188 ILEFLRGQGSPLMVSVYTYDQYVYG-GNISLGYATLSEQNTLVRDGMLSYNNLFDEMVDA 247
           +L+ L    SP++V +Y Y  Y     +I L YAT + +  +V+DG L Y+N+FD + DA
Sbjct: 208 VLKLLSQTNSPILVKIYPYFSYASDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDA 267

Query: 248 FYAAMDKENVGDVAIVFGETGWPTCGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIY 307
           F  AM+KE V D+ +V  ETGWP+ GN N TTP +A  +NRNF  HI+SGKGTPK+ N  
Sbjct: 268 FVWAMEKEGVKDLPMVVSETGWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKG 327

Query: 308 IEGFIRSIFNENQKPEGESRCYGMFNV-DSTPIYSV 337
           I+GF+ + FNENQKP G  + +G++N  D  PIY++
Sbjct: 328 IDGFLFATFNENQKPVGTEQNFGLYNPNDMKPIYNL 353

BLAST of Cp4.1LG18g00200 vs. Swiss-Prot
Match: E13B_HORVU (Glucan endo-1,3-beta-glucosidase GII OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 2.3e-49
Identity = 123/333 (36.94%), Postives = 183/333 (54.95%), Query Frame = 1

Query: 18  MAAMSLVVGAYDVL------LGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNLDVI 77
           M A +L +GA+  +      +G  YG++G+NLPS S VVQL R   I+ +R+   +   +
Sbjct: 9   MFAAALFIGAFAAVPTSVQSIGVCYGVIGNNLPSRSDVVQLYRSKGINGMRIYFADGQAL 68

Query: 78  EGFRGTQIDLSFGVPNDMLPNMA---SNKTLWFNTYVEPYTNDCTINYIVVGDKAIPGLD 137
              R + I L   + ND L N+A   SN   W    V PY     I YI  G++   G  
Sbjct: 69  SALRNSGIGLILDIGNDQLANIAASTSNAASWVQNNVRPYYPAVNIKYIAAGNEVQGGAT 128

Query: 138 DNILPVMKSLQDLLNARYFGAVKLTTLVGFNALAVTNPPSSGAFNPVIVENMRGILEFLR 197
            +ILP M++L   L+A   GA+K++T + F+ +A + PPS+G F       M  +   L 
Sbjct: 129 QSILPAMRNLNAALSAAGLGAIKVSTSIRFDEVANSFPPSAGVFKNAY---MTDVARLLA 188

Query: 198 GQGSPLMVSVYTYDQYVYG-GNISLGYATLSEQNTLVRD--GMLSYNNLFDEMVDAFYAA 257
             G+PL+ +VY Y  Y    G+ISL YAT  +  T VRD    L+Y +LFD MVDA YAA
Sbjct: 189 STGAPLLANVYPYFAYRDNPGSISLNYATF-QPGTTVRDQNNGLTYTSLFDAMVDAVYAA 248

Query: 258 MDKENVGDVAIVFGETGWPTCGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIYIEGF 317
           ++K     V +V  E+GWP+ G + A+    A  +N+   +H+  G GTPKK    +E +
Sbjct: 249 LEKAGAPAVKVVVSESGWPSAGGFAASAG-NARTYNQGLINHV--GGGTPKKREA-LETY 308

Query: 318 IRSIFNENQKP-EGESRCYGMFNVDSTPIYSVE 338
           I ++FNENQK  +   R +G+FN D +P Y+++
Sbjct: 309 IFAMFNENQKTGDATERSFGLFNPDKSPAYNIQ 333

BLAST of Cp4.1LG18g00200 vs. Swiss-Prot
Match: GUB2_HORVU (Lichenase-2 (Fragment) OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 5.0e-49
Identity = 108/309 (34.95%), Postives = 171/309 (55.34%), Query Frame = 1

Query: 32  LGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNLDVIEGFRGTQIDLSFGVPNDMLP 91
           +G  YG+  +NLP+ S+VV + +   I  +RL  PN   ++   GT I++  G PND+L 
Sbjct: 7   IGVCYGMSANNLPAASTVVSMFKFNGIKSMRLYAPNQAALQAVGGTGINVVVGAPNDVLS 66

Query: 92  NMASNKTL---WFNTYVEPYTNDCTINYIVVGDKAIPGLDDNILPVMKSLQDLLNARYFG 151
           N+A++      W  + ++ Y    +  Y+ VG++   G   N++P MK++   L A   G
Sbjct: 67  NLAASPAAAASWVKSNIQAYPK-VSFRYVCVGNEVAGGATRNLVPAMKNVHGALVAAGLG 126

Query: 152 AVKLTTLVGFNALAVTNPPSSGAFNPVIVENMRGILEFLRGQGSPLMVSVYTYDQYVYGG 211
            +K+TT V    L V +PPS+G+F       M  +++FL    +PLM ++Y Y  + Y  
Sbjct: 127 HIKVTTSVSQAILGVFSPPSAGSFTGEAAAFMGPVVQFLARTNAPLMANIYPYLAWAYNP 186

Query: 212 N-ISLGYATLSEQNTLVRDGMLSYNNLFDEMVDAFYAAMDKENVGDVAIVFGETGWPTCG 271
           + + +GYA  +   T+VRDG   Y NLFD  VDAFY AM K     V +V  E+GWP+ G
Sbjct: 187 SAMDMGYALFNASGTVVRDGAYGYQNLFDTTVDAFYTAMGKHGGSSVKLVVSESGWPS-G 246

Query: 272 NYNATTPLLAAEFNRNFKSHISSGKGTPKKENIYIEGFIRSIFNENQKPEGESRCYGMFN 331
              A TP  A  +N++  +H+  G+GTP+     IE +I ++FNENQK  G  + +G+F 
Sbjct: 247 GGTAATPANARFYNQHLINHV--GRGTPRHPGA-IETYIFAMFNENQKDSGVEQNWGLFY 306

Query: 332 VDSTPIYSV 337
            +   +Y +
Sbjct: 307 PNMQHVYPI 310

BLAST of Cp4.1LG18g00200 vs. Swiss-Prot
Match: E13A_SOYBN (Glucan endo-1,3-beta-glucosidase OS=Glycine max PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.5e-48
Identity = 116/323 (35.91%), Postives = 180/323 (55.73%), Query Frame = 1

Query: 26  GAYDVLLGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNLDVIEGFRGTQIDLSFGV 85
           G  D   G  YG LG+NLP+P  VV L  + NI R+R+  P+ +V+E  RG+ I+L   +
Sbjct: 28  GTTDAQSGVCYGRLGNNLPTPQEVVALYNQANIRRMRIYGPSPEVLEALRGSNIELLLDI 87

Query: 86  PNDMLPNMAS---NKTLWFNTYVEPYTNDCTINYIVVGDKAIP--GLDDNILPVMKSLQD 145
           PND L N+AS   N   W    ++ Y N+    Y+ VG++  P       ++P ++++Q 
Sbjct: 88  PNDNLRNLASSQDNANKWVQDNIKNYANNVRFRYVSVGNEVKPEHSFAQFLVPALENIQR 147

Query: 146 LL-NARYFGAVKLTTLVGFNALAVTNPPSSGAF-NPVIVENMRGILEFLRGQGSPLMVSV 205
            + NA     VK++T +   ALA + PPS G+F +      + G++ FL    +PLMV+V
Sbjct: 148 AISNAGLGNQVKVSTAIDTGALAESFPPSKGSFKSDYRGAYLDGVIRFLVNNNAPLMVNV 207

Query: 206 YTYDQYVYG-GNISLGYATLSEQNTLVRDGMLSYNNLFDEMVDAFYAAMDKENVGDVAIV 265
           Y+Y  Y     +ISL YA     + +V+DG L Y NLFD  VDA YAA++K   G + IV
Sbjct: 208 YSYFAYTANPKDISLDYALFRSPSVVVQDGSLGYRNLFDASVDAVYAALEKAGGGSLNIV 267

Query: 266 FGETGWPTCGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIYIEGFIRSIFNENQKPE 325
             E+GWP+ G   AT+   A  +N N   ++   +GTPK+    +E ++ ++F+ENQK  
Sbjct: 268 VSESGWPSSGG-TATSLDNARTYNTNLVRNVK--QGTPKRPGAPLETYVFAMFDENQKQP 327

Query: 326 GESRCYGMFN-VDSTPIYSVEQN 340
              + +G+F+ +   P YS+  N
Sbjct: 328 EFEKFWGLFSPITKQPKYSINFN 347

BLAST of Cp4.1LG18g00200 vs. TrEMBL
Match: A0A0A0LR03_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G416760 PE=3 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 7.0e-114
Identity = 213/330 (64.55%), Postives = 255/330 (77.27%), Query Frame = 1

Query: 16  LFMAAMSLVVG-AYDVLLGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNLDVIEGF 75
           LFM AMS +V    ++ +GAYYG  G+NLP P  VVQLC KYNI R+R   PNLD++E F
Sbjct: 12  LFMVAMSSIVARGNNIFVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRFNEPNLDILEQF 71

Query: 76  RGTQIDLSFGVPNDMLPNMASNKTL---WFNTYVEPYTNDCTINYIVVGDKAIPGLDDNI 135
           RGT I++SF VP +++ N A+N+T    WF  YV P+  + TINYIVVGDK IPGLDDNI
Sbjct: 72  RGTNIEVSFSVPGELIINQATNQTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLDDNI 131

Query: 136 LPVMKSLQDLLNARYFGAVKLTTLVGFNALAVTNPPSSGAFNPVIVENMRGILEFLRGQG 195
           LPVM+SLQDLLN+RY G VK+TT+VG  AL V +PPSSGAF+P ++ENM+GIL+FL GQG
Sbjct: 132 LPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFLWGQG 191

Query: 196 SPLMVSVYTYDQYVYGG---NISLGYATL---SEQNTLVR-DGMLSYNNLFDEMVDAFYA 255
           SPLM+S+Y YD Y Y G   NISLGYAT    +EQN  +R DG L Y N+FD+MVDAF+A
Sbjct: 192 SPLMLSLYPYDAYAYTGYTNNISLGYATFTSQTEQNPPIRIDGNLYYKNVFDDMVDAFHA 251

Query: 256 AMDKENVGDVAIVFGETGWPTCGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIYIEG 315
           A+DK   GDVAI  GETGWPT GNY A  P LA  +N NFK+HI+SGKGTP K NIYIEG
Sbjct: 252 AIDKACAGDVAIAIGETGWPTEGNYGA-GPSLALTYNLNFKNHITSGKGTPMKPNIYIEG 311

Query: 316 FIRSIFNENQKPEGESRCYGMFNVDSTPIY 335
           FIRS+FNEN+KPEGES+ YGMF+VDSTPIY
Sbjct: 312 FIRSLFNENEKPEGESQFYGMFHVDSTPIY 340

BLAST of Cp4.1LG18g00200 vs. TrEMBL
Match: A0A0A0LZ10_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G595900 PE=3 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 4.2e-111
Identity = 203/333 (60.96%), Postives = 260/333 (78.08%), Query Frame = 1

Query: 6   KMVKISHMIGLFMAAMSLVVGAYDVLLGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEV 65
           K+V+I  ++   + A + VVGAYD+LLGAYYGL+GDNLP P  VVQLC +YNI RVRL+V
Sbjct: 3   KVVQIVFLMASILMAAT-VVGAYDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDV 62

Query: 66  PNLDVIEGFRGTQIDLSFGVPNDMLPNMASNKTL---WFNTYVEPYTNDCTINYIVVGDK 125
           P++DV+  FRG  ID+SFG+PN+ML ++A+NK L   WFNTYV+ + +D  INYI+VGDK
Sbjct: 63  PDVDVLTAFRGGAIDVSFGIPNNMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDK 122

Query: 126 AIPGLDDNILPVMKSLQDLLNARYFGAVKLTTLVGFNALAVT-NPPSSGAFNPVIVENMR 185
           AIP   + ILP+M SLQDLLNA Y G VKLTTLVG+NA  V+ +PPSSG F+P + E+MR
Sbjct: 123 AIPSHANFILPIMMSLQDLLNANYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMR 182

Query: 186 GILEFLRGQGSPLMVSVYTYDQYVYGGNISLGYATLSEQNTLVRDGMLSYNNLFDEMVDA 245
           GIL+FL  +GS LMVSV+ + +Y + G ISL YA  +E N +V+D  L Y NLFD MVDA
Sbjct: 183 GILKFLAEEGSALMVSVFPHQKYEFNGGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDA 242

Query: 246 FYAAMDKENVGDVAIVFGETGWPTCGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIY 305
           FYAA++KE VG+V IV GETGWPTCG+ +  T  +A E+N  FKSHISSG GTP+K N+Y
Sbjct: 243 FYAAIEKEMVGEVNIVVGETGWPTCGDISG-TQAIAKEYNNKFKSHISSGNGTPRKPNVY 302

Query: 306 IEGFIRSIFNENQKPEGESRCYGMFNVDSTPIY 335
           +EGFI+SIFNE++KPEG+S+CYGMF+V+  PIY
Sbjct: 303 LEGFIQSIFNEDKKPEGDSQCYGMFDVNMKPIY 333

BLAST of Cp4.1LG18g00200 vs. TrEMBL
Match: A0A0A0LWM4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G595910 PE=3 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.0e-96
Identity = 182/270 (67.41%), Postives = 214/270 (79.26%), Query Frame = 1

Query: 75  RGTQIDLSFGVPNDMLPNMASNKTL---WFNTYVEPYTNDCTINYIVVGDKAIPGLDDNI 134
           RGT I++SF VP +++ N A+N T    WF  YV P+  + TINYIVVGDK IPGLDDNI
Sbjct: 7   RGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLDDNI 66

Query: 135 LPVMKSLQDLLNARYFGAVKLTTLVGFNALAVTNPPSSGAFNPVIVENMRGILEFLRGQG 194
           LPVM+SLQDLLN+RY G VK+TT+VG  AL V +PPSSGAF+P ++ENM+GIL+FL GQG
Sbjct: 67  LPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFLWGQG 126

Query: 195 SPLMVSVYTYDQYVYGG---NISLGYATL---SEQNTLVR-DGMLSYNNLFDEMVDAFYA 254
           SPLM+S+Y YD Y Y G   NISLGYAT    +EQN  +R DG LSYNN+FDEMVDAFYA
Sbjct: 127 SPLMLSLYPYDAYAYTGYTNNISLGYATFTSQTEQNPPIRTDGDLSYNNIFDEMVDAFYA 186

Query: 255 AMDKENVGDVAIVFGETGWPTCGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIYIEG 314
           A+DK NVGDVAI  GETGWPT GNY A  P LA  +N NFK+HI+SGKGTP   NIYIEG
Sbjct: 187 AIDKANVGDVAIAIGETGWPTNGNYGA-GPSLALTYNLNFKNHITSGKGTPMMPNIYIEG 246

Query: 315 FIRSIFNENQKPEGESRCYGMFNVDSTPIY 335
           FIRS+FNEN+KPEGES+ YGMF++D TPIY
Sbjct: 247 FIRSLFNENEKPEGESQFYGMFHIDFTPIY 275

BLAST of Cp4.1LG18g00200 vs. TrEMBL
Match: M5VWE6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa017802mg PE=3 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 2.5e-71
Identity = 146/327 (44.65%), Postives = 209/327 (63.91%), Query Frame = 1

Query: 17  FMAAMSLVVGAYD--VLLGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNLDVIEGF 76
           F+AA    +G  +  + +G  YG+LG++LPSP+ VV L + Y I ++RL  PN + ++  
Sbjct: 13  FVAASHNRIGLVEASIDIGVCYGMLGNDLPSPTQVVNLYKTYGIGKMRLFDPNPEALQAL 72

Query: 77  RGTQIDLSFGVPNDMLPNMASNKTL---WFNTYVEPYTNDCTINYIVVGDKAIPGLDDN- 136
           RG+QID+S G+ N+ LP++A ++     WF T+VEPY ND   N+I VG++ IPG   N 
Sbjct: 73  RGSQIDVSIGIRNEDLPSLAVSQDAVNSWFATHVEPYLNDIVFNHITVGNEIIPGSSGNY 132

Query: 137 ILPVMKSLQDLLNARYFGAVKLTTLVGFNALAVTNPPSSGAFNPVIVENMRGILEFLRGQ 196
           +LPVM+ LQ +L+ R    +K+TT+V   AL V+ PPSSGAF       M GIL  L  +
Sbjct: 133 VLPVMQYLQKILDDRSLAGIKVTTVVSGAALGVSYPPSSGAFTLEASSTMSGILPLLAQR 192

Query: 197 GSPLMVSVYTYDQYVYGG-NISLGYATLSEQNTLVRDGMLSYNNLFDEMVDAFYAAMDKE 256
           GSPLM++VY Y  Y     N+ L YA  +  + +V+DG LSY NLFD  VDAF AAM+K 
Sbjct: 193 GSPLMINVYPYFAYASDPVNVHLDYAQFTATSPVVQDGTLSYYNLFDATVDAFIAAMEKV 252

Query: 257 NVGDVAIVFGETGWPTCGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIYIEGFIRSI 316
              +V +V  E+GWP+ GN + TTP LA  +NRN    ++S  GTPK+   YIE +I ++
Sbjct: 253 GGANVDVVVSESGWPSDGNGDFTTPELAGTYNRNLLKQVTSRAGTPKRPGAYIEAYIFAM 312

Query: 317 FNENQKPEGESRCYGMFNVDSTPIYSV 337
           FNENQKP+G  + +G+F+ +  P+Y V
Sbjct: 313 FNENQKPDGVEQHFGLFHPNMKPVYPV 339

BLAST of Cp4.1LG18g00200 vs. TrEMBL
Match: M5VS03_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa015919mg PE=3 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 9.5e-71
Identity = 147/321 (45.79%), Postives = 204/321 (63.55%), Query Frame = 1

Query: 21  MSLVVGAYDVLLGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNLDVIEGFRGTQID 80
           + LV  + D+  G  YG+LGD+LP+ + VV L +   I ++RL  PN   +E  RG +ID
Sbjct: 21  IGLVEASVDI--GVCYGMLGDDLPAATEVVNLYKINGIGKMRLFDPNPAALEALRGKEID 80

Query: 81  LSFGVPNDMLPNMASNKTL---WFNTYVEPYTNDCTINYIVVGDKAIPG-LDDNILPVMK 140
           +S G+ N+ LP +  +      WF T VEPY ND   NYI VG++ IPG L   +LPVM+
Sbjct: 81  VSLGIRNEDLPQLTGSADAVNSWFATNVEPYLNDIVFNYISVGNEVIPGSLGIYVLPVMQ 140

Query: 141 SLQDLLNARYFGAVKLTTLVGFNALAVTNPPSSGAFNPVIVENMRGILEFLRGQGSPLMV 200
           SLQ +L+ R    +K++T+V  +AL V+ PPSSG F       M GI+ FL  +GSPL++
Sbjct: 141 SLQKILDDRNLAGIKVSTVVPGSALGVSFPPSSGEFALEASSVMSGIVAFLAQRGSPLLI 200

Query: 201 SVYTYDQYVYGG-NISLGYATLSEQNTLVRDGMLSYNNLFDEMVDAFYAAMDKENVGDVA 260
           +VY Y  Y     NI L YA  +  + +V+DG LSY NLFD  VD+F AAM+K    +V 
Sbjct: 201 NVYPYFSYASDPVNIRLDYAQFTATSPVVKDGELSYYNLFDAAVDSFLAAMEKVGGANVD 260

Query: 261 IVFGETGWPTCGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIYIEGFIRSIFNENQK 320
           +V  E+GWP+ GN N TTP LA  +NRNF  HI+S  GTPK+   YIEG+I ++FNENQK
Sbjct: 261 VVVSESGWPSDGNGNFTTPELAGTYNRNFLKHITSNAGTPKRPGAYIEGYIFAMFNENQK 320

Query: 321 PEGESRCYGMFNVDSTPIYSV 337
           PEG  + +G+F+ +  P+YS+
Sbjct: 321 PEGVEQHFGLFHPNMQPVYSI 339

BLAST of Cp4.1LG18g00200 vs. TAIR10
Match: AT5G20330.1 (AT5G20330.1 beta-1,3-glucanase 4)

HSP 1 Score: 248.4 bits (633), Expect = 8.2e-66
Identity = 129/312 (41.35%), Postives = 198/312 (63.46%), Query Frame = 1

Query: 32  LGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNLDVIEGFRGTQ-IDLSFGVPNDML 91
           +G  YGLLGDNLPSPS+V+ L +   ISR+R+  PN +V+   RG + I+++ GV +  L
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 92  PNMASNKTL---WFNTYVEPYTNDCTINYIVVGDKAIPG-LDDNILPVMKSLQDLLNARY 151
             +A+++     WF   +E Y  D  I +I VG++ IPG +   +LPVM+SL +L+ +R 
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 153

Query: 152 FGAVKLTTLVGFNALAVTNPPSSGAFNPVIVENMRGILEFLRGQGSPLMVSVYTYDQYVY 211
              + ++T+V  + L  + PPS+G F     E +  +L+ L    +P++V++Y Y  Y  
Sbjct: 154 L-PISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYFAYAS 213

Query: 212 G-GNISLGYATLSEQNTLVRDGMLSYNNLFDEMVDAFYAAMDKENVGDVAIVFGETGWPT 271
              NI L YA+ + ++ +V+DG L Y+N+FD + DAF  AM+KE V ++ +V  ETGWP+
Sbjct: 214 DPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGWPS 273

Query: 272 CGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIYIEGFIRSIFNENQKPEGESRCYGM 331
            GN N TTP +A+ +NRNF  HI+SGKGTPK+ N  + GF+ + FNENQKP G  + +G+
Sbjct: 274 AGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQNFGL 333

Query: 332 FN-VDSTPIYSV 337
           +N  D  PIY +
Sbjct: 334 YNPSDMKPIYKL 344

BLAST of Cp4.1LG18g00200 vs. TAIR10
Match: AT5G20340.1 (AT5G20340.1 beta-1,3-glucanase 5)

HSP 1 Score: 246.9 bits (629), Expect = 2.4e-65
Identity = 137/336 (40.77%), Postives = 206/336 (61.31%), Query Frame = 1

Query: 8   VKISHMIGLFMAAMSLVVGAYDVLLGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPN 67
           V  ++ IG   AA S+         G  YGLLGDNLPSPS V+ L +  +I+++R+  PN
Sbjct: 28  VNAANSIGFVNAANSI---------GLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPN 87

Query: 68  LDVIEGFRGTQ-IDLSFGVPNDMLPNMASNKTL---WFNTYVEPYTNDCTINYIVVGDKA 127
            +V+   RG + I ++ GV +  L  +++++     WF T +EPY +D  I +I VG++ 
Sbjct: 88  TEVLNALRGHRDIAVTVGVRDQDLAALSASEEAVKGWFATNIEPYLSDINIAFITVGNEV 147

Query: 128 IPG-LDDNILPVMKSLQDLLNARYFGAVKLTTLVGFNALAVTNPPSSGAFNPVIVENMRG 187
           IPG +   +LPVM+SL +L+ +R    + ++T+V    L  + PPS+G F     E +  
Sbjct: 148 IPGPIGPQVLPVMQSLTNLVKSRNL-PISISTVVAMWNLEQSYPPSAGMFTSQAREQLVP 207

Query: 188 ILEFLRGQGSPLMVSVYTYDQYVYG-GNISLGYATLSEQNTLVRDGMLSYNNLFDEMVDA 247
           +L+ L    SP++V +Y Y  Y     +I L YAT + +  +V+DG L Y+N+FD + DA
Sbjct: 208 VLKLLSQTNSPILVKIYPYFSYASDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDA 267

Query: 248 FYAAMDKENVGDVAIVFGETGWPTCGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIY 307
           F  AM+KE V D+ +V  ETGWP+ GN N TTP +A  +NRNF  HI+SGKGTPK+ N  
Sbjct: 268 FVWAMEKEGVKDLPMVVSETGWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKG 327

Query: 308 IEGFIRSIFNENQKPEGESRCYGMFNV-DSTPIYSV 337
           I+GF+ + FNENQKP G  + +G++N  D  PIY++
Sbjct: 328 IDGFLFATFNENQKPVGTEQNFGLYNPNDMKPIYNL 353

BLAST of Cp4.1LG18g00200 vs. TAIR10
Match: AT5G20390.1 (AT5G20390.1 Glycosyl hydrolase superfamily protein)

HSP 1 Score: 239.6 bits (610), Expect = 3.8e-63
Identity = 127/312 (40.71%), Postives = 186/312 (59.62%), Query Frame = 1

Query: 32  LGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNLDVIEGFRGTQ-IDLSFGVPNDML 91
           +G  YGLLGDNLP PS V+ L +  +++ +R+     DV+  FRG + I +   V N  L
Sbjct: 33  IGLNYGLLGDNLPPPSEVINLYKSLSVTNIRIFDTTTDVLNAFRGNRNIGVMVDVKNQDL 92

Query: 92  PNMASNK---TLWFNTYVEPYTNDCTINYIVVGDKAIPG-LDDNILPVMKSLQDLLNARY 151
             ++ ++     WF T +EPY  D  I +I VG++ IPG +   +LPVMKSL +++ +R 
Sbjct: 93  EALSVSEEAVNTWFVTNIEPYLADVNITFIAVGNEVIPGEIGSYVLPVMKSLTNIVKSRS 152

Query: 152 FGAVKLTTLVGFNALAVTNPPSSGAFNPVIVENMRGILEFLRGQGSPLMVSVYTYDQYVY 211
              + ++T V    L  + PPS+G F P   E +  +L+FL    +P++V++Y Y  Y  
Sbjct: 153 L-PILISTTVAMTNLGQSYPPSAGDFMPQAREQLTPVLKFLSQTNTPILVNIYPYFAYAA 212

Query: 212 GG-NISLGYATLSEQNTLVRDGMLSYNNLFDEMVDAFYAAMDKENVGDVAIVFGETGWPT 271
              NI L YA  +    +V+DG L Y N+FD + DAF  AM+KE V D+ +V  ETGWP+
Sbjct: 213 DPINIQLDYAIFNTNKVVVQDGPLGYTNMFDVIFDAFVWAMEKEGVKDLPMVVTETGWPS 272

Query: 272 CGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIYIEGFIRSIFNENQKPEGESRCYGM 331
            GN N TTP +A+ +N NF  H+ SGKGTPK+    I GF+ + FNENQKP G  + +G+
Sbjct: 273 AGNGNLTTPDIASIYNTNFVKHVESGKGTPKRPKSGISGFLFATFNENQKPAGTEQNFGL 332

Query: 332 FN-VDSTPIYSV 337
           +N  D  PIY +
Sbjct: 333 YNPTDMKPIYKM 343

BLAST of Cp4.1LG18g00200 vs. TAIR10
Match: AT5G20560.1 (AT5G20560.1 Glycosyl hydrolase superfamily protein)

HSP 1 Score: 234.2 bits (596), Expect = 1.6e-61
Identity = 125/328 (38.11%), Postives = 196/328 (59.76%), Query Frame = 1

Query: 16  LFMAAMSLVVGAYDV-------LLGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNL 75
           L  +  +L++  Y+V       ++G  YGLLGDNLPSPS+V++  +  N++++R+  PN 
Sbjct: 11  LLFSCTALIISYYNVDSLSTASVVGLNYGLLGDNLPSPSNVIKFYKSQNVAKIRIFEPNK 70

Query: 76  DVIEGFRGTQ-IDLSFGVPNDMLPNMASNKTL---WFNTYVEPYTNDCTINYIVVGDKAI 135
           DV+   RG + I ++ G+ N+ L  +A+NK     WF+T ++PY  D  I +I VG++AI
Sbjct: 71  DVLNALRGNRDIGVTVGIKNEDLEALAANKDAVKSWFSTNIDPYIADVNITFITVGNQAI 130

Query: 136 PG--LDDNILPVMKSLQDLLNARYFGAVKLTTLVGFNALAVTNPPSSGAFNPVIVENMRG 195
           PG     ++LPV++SL DL+ +R    + ++T V   +LA   PPS+G   P   + +  
Sbjct: 131 PGDKHGPHVLPVIQSLTDLVKSRNL-QISISTTVTTTSLARLKPPSAGVLTPQARQQLVP 190

Query: 196 ILEFLRGQGSPLMVSVYTYDQYVYGG---NISLGYATLSEQNTLVRDGMLSYNNLFDEMV 255
           +L  L    +P+ V++Y Y  Y +     N+ L YA  +    +V+DG L Y+NLFD + 
Sbjct: 191 VLRLLSQTSTPIFVNIYPY--YFHASDPKNVPLEYANFNNDQIVVKDGALKYSNLFDAIF 250

Query: 256 DAFYAAMDKENVGDVAIVFGETGWPTCGNYNATTPLLAAEFNRNFKSHISSGKGTPKKEN 315
           DAF  AM+KE V  + +V  ETGWP+ GN   TTP L   +  NF  H++SGKGTPK+ N
Sbjct: 251 DAFLWAMEKEGVKGLPLVVSETGWPSAGNGGMTTPALQYTYIGNFVKHVASGKGTPKRPN 310

Query: 316 IYIEGFIRSIFNENQKPEGESRCYGMFN 328
             I+ +I   +NENQKP G  + +G+++
Sbjct: 311 SRIDAYIFETYNENQKPVGIYQHFGLYD 335

BLAST of Cp4.1LG18g00200 vs. TAIR10
Match: AT1G33220.1 (AT1G33220.1 Glycosyl hydrolase superfamily protein)

HSP 1 Score: 233.0 bits (593), Expect = 3.6e-61
Identity = 126/311 (40.51%), Postives = 189/311 (60.77%), Query Frame = 1

Query: 32  LGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNLDVIEGFRGTQ-IDLSFGVPNDML 91
           +G  YGLLGDN P+PS+V+ L +   I+++R+  P  +V+   RG + I+++ GV +  L
Sbjct: 34  IGLNYGLLGDNFPTPSNVLNLYKSIGITKIRIFDPKTEVLNALRGHRNIEVTVGVRDQDL 93

Query: 92  PNMASNKTL---WFNTYVEPYTNDCTINYIVVGDKAIPG-LDDNILPVMKSLQDLLNARY 151
             +++N+     WF T +EPY  D  I +I VG++ IPG +   +LPVM+SL  L+ +  
Sbjct: 94  GALSANEEAVKGWFATNIEPYLADVNIAFITVGNEVIPGPIGPQVLPVMQSLTILVKSMN 153

Query: 152 FGAVKLTTLVGFNALAVTNPPSSGAFNPVIVENMRGILEFLRGQGSPLMVSVYTYDQYVY 211
              + ++T+V  + L  + PPS+G F     E +  +L+ L    +P++V++Y Y  Y  
Sbjct: 154 L-PISISTVVAMSNLEQSYPPSAGEFTSQAREQLVPVLKLLSQTSTPILVNIYPYFPYAS 213

Query: 212 GG-NISLGYATLSEQNTLVRDGMLSYNNLFDEMVDAFYAAMDKENVGDVAIVFGETGWPT 271
              NI L YAT + + T+V+DG L Y+N+FD + DAF  AM+KE V D+ +V  ETGWP+
Sbjct: 214 DPTNIPLDYATFNTKATVVQDGPLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWPS 273

Query: 272 CGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIYIEGFIRSIFNENQKPEGESRCYGM 331
            GN N TTP +A  +NRNF  HI SGKGTPK+ N  ++GF+ + FNENQKP G       
Sbjct: 274 AGNGNLTTPDIAGTYNRNFVKHIVSGKGTPKRPNNGMDGFLFATFNENQKPAG------- 333

Query: 332 FNVDSTPIYSV 337
              D  PIY +
Sbjct: 334 --TDMKPIYKL 334

BLAST of Cp4.1LG18g00200 vs. NCBI nr
Match: gi|659100571|ref|XP_008451158.1| (PREDICTED: uncharacterized protein LOC103492536 [Cucumis melo])

HSP 1 Score: 443.7 bits (1140), Expect = 3.8e-121
Identity = 225/329 (68.39%), Postives = 255/329 (77.51%), Query Frame = 1

Query: 17  FMAAMSLVVGAYDVLLGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNLDVIEGFRG 76
           F A  S+VV AYDVLLGAYYG  G+NLP P  VVQLC+KYNI R+R   PN D+I+ FRG
Sbjct: 325 FYAMSSIVVRAYDVLLGAYYGTEGNNLPPPEKVVQLCQKYNIRRIRFSEPNFDIIDAFRG 384

Query: 77  TQIDLSFGVPNDMLPNMASNKTL---WFNTYVEPYTNDCTINYIVVGDKAIPGLDDNILP 136
             I+LSF V  +++ NMA+N T    WF  YV P+  D TINYI+VGDKAIPGLDDNILP
Sbjct: 385 KDIELSFSVTGELITNMATNHTAVEEWFVNYVAPFIGDFTINYIIVGDKAIPGLDDNILP 444

Query: 137 VMKSLQDLLNARYFGAVKLTTLVGFNALAVTNPPSSGAFNPVIVENMRGILEFLRGQGSP 196
           VMKSLQ LLN RY G VK+TTLVG  AL V  PPSSG F+P ++ENMRGIL+FL GQGSP
Sbjct: 445 VMKSLQVLLNGRYLGQVKITTLVGLAALGVQTPPSSGTFDPNVLENMRGILQFLHGQGSP 504

Query: 197 LMVSVYTYDQYVYGG---NISLGYATLS---EQNTLVRD-GMLSYNNLFDEMVDAFYAAM 256
           LM+S+Y Y +Y Y G   NISLGYA  +   E+N  +R  G LSYNNLFDEMVD FYAA+
Sbjct: 505 LMLSLYPYQEYAYTGNSNNISLGYANFNSQLEKNPPIRTYGDLSYNNLFDEMVDTFYAAI 564

Query: 257 DKENVGDVAIVFGETGWPTCGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIYIEGFI 316
           DK NVGDV IV GETGWPT GNY   +P LAA +NRNFK+HISSGKGTP K NIYIEGFI
Sbjct: 565 DKANVGDVPIVIGETGWPTNGNYGG-SPSLAATYNRNFKNHISSGKGTPMKPNIYIEGFI 624

Query: 317 RSIFNENQKPEGESRCYGMFNVDSTPIYS 336
           RS+FNEN+KPEGESR YGMFNVDSTPIYS
Sbjct: 625 RSLFNENEKPEGESRFYGMFNVDSTPIYS 652

BLAST of Cp4.1LG18g00200 vs. NCBI nr
Match: gi|659100567|ref|XP_008451156.1| (PREDICTED: putative glucan endo-1,3-beta-glucosidase GVI [Cucumis melo])

HSP 1 Score: 426.0 bits (1094), Expect = 8.2e-116
Identity = 208/338 (61.54%), Postives = 263/338 (77.81%), Query Frame = 1

Query: 7   MVKISHMIGLFMAAMSL---VVGAYDVLLGAYYGLLGDNLPSPSSVVQLCRKYNISRVRL 66
           M K+  +I   MA++ +   +V A+DVLLGAYYGL G+NLP P  VV LC KYNI R+RL
Sbjct: 1   MAKLVQIIVFLMASILMPTSLVRAHDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRL 60

Query: 67  EVPNLDVIEGFRGTQIDLSFGVPNDMLPNMASNKTL---WFNTYVEPYTNDCTINYIVVG 126
           ++P +DV+  FRG+ IDLSF VPNDML +MA+NK L   WFNTY++P+ +D  INYI+VG
Sbjct: 61  DIPTVDVLTAFRGSNIDLSFSVPNDMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVG 120

Query: 127 DKAIPGLDDNILPVMKSLQDLLNARYFGAVKLTTLVGFNALAVT-NPPSSGAFNPVIVEN 186
           DKA+PGLD+ ILPVM SLQDLLNA YFG VKLTTLVG+NA  V+ +PPSSG FNP + EN
Sbjct: 121 DKAVPGLDNFILPVMMSLQDLLNANYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVREN 180

Query: 187 MRGILEFLRGQGSPLMVSVYTYDQYVYGGNISLGYATLSEQNTLVRDGMLSYNNLFDEMV 246
           MRGIL+FL  +GS LMVSV+ + QY   G +SL YA L E   +V DG L Y+NLFD M+
Sbjct: 181 MRGILKFLAEEGSALMVSVFPHRQYKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMM 240

Query: 247 DAFYAAMDKENVGDVAIVFGETGWPTCG-NYNATTPLLAAEFNRNFKSHISSGKGTPKKE 306
           DAFYAA++KE +G+V +V GETGWPTCG N+   T  +A ++N  FKSHISSGKGTP+K 
Sbjct: 241 DAFYAAIEKETIGEVTLVVGETGWPTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKP 300

Query: 307 NIYIEGFIRSIFNENQKPEGESRCYGMFNVDSTPIYSV 337
           N+Y+EGFI+S+FNENQKPEGES+C+G+F+V+  PIYS+
Sbjct: 301 NVYLEGFIQSLFNENQKPEGESQCFGVFDVNMNPIYSL 338

BLAST of Cp4.1LG18g00200 vs. NCBI nr
Match: gi|778674443|ref|XP_004153772.2| (PREDICTED: lichenase-2-like [Cucumis sativus])

HSP 1 Score: 419.1 bits (1076), Expect = 1.0e-113
Identity = 213/330 (64.55%), Postives = 255/330 (77.27%), Query Frame = 1

Query: 16  LFMAAMSLVVG-AYDVLLGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEVPNLDVIEGF 75
           LFM AMS +V    ++ +GAYYG  G+NLP P  VVQLC KYNI R+R   PNLD++E F
Sbjct: 12  LFMVAMSSIVARGNNIFVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRFNEPNLDILEQF 71

Query: 76  RGTQIDLSFGVPNDMLPNMASNKTL---WFNTYVEPYTNDCTINYIVVGDKAIPGLDDNI 135
           RGT I++SF VP +++ N A+N+T    WF  YV P+  + TINYIVVGDK IPGLDDNI
Sbjct: 72  RGTNIEVSFSVPGELIINQATNQTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLDDNI 131

Query: 136 LPVMKSLQDLLNARYFGAVKLTTLVGFNALAVTNPPSSGAFNPVIVENMRGILEFLRGQG 195
           LPVM+SLQDLLN+RY G VK+TT+VG  AL V +PPSSGAF+P ++ENM+GIL+FL GQG
Sbjct: 132 LPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFLWGQG 191

Query: 196 SPLMVSVYTYDQYVYGG---NISLGYATL---SEQNTLVR-DGMLSYNNLFDEMVDAFYA 255
           SPLM+S+Y YD Y Y G   NISLGYAT    +EQN  +R DG L Y N+FD+MVDAF+A
Sbjct: 192 SPLMLSLYPYDAYAYTGYTNNISLGYATFTSQTEQNPPIRIDGNLYYKNVFDDMVDAFHA 251

Query: 256 AMDKENVGDVAIVFGETGWPTCGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIYIEG 315
           A+DK   GDVAI  GETGWPT GNY A  P LA  +N NFK+HI+SGKGTP K NIYIEG
Sbjct: 252 AIDKACAGDVAIAIGETGWPTEGNYGA-GPSLALTYNLNFKNHITSGKGTPMKPNIYIEG 311

Query: 316 FIRSIFNENQKPEGESRCYGMFNVDSTPIY 335
           FIRS+FNEN+KPEGES+ YGMF+VDSTPIY
Sbjct: 312 FIRSLFNENEKPEGESQFYGMFHVDSTPIY 340

BLAST of Cp4.1LG18g00200 vs. NCBI nr
Match: gi|778665329|ref|XP_004144124.2| (PREDICTED: putative glucan endo-1,3-beta-glucosidase GVI [Cucumis sativus])

HSP 1 Score: 409.8 bits (1052), Expect = 6.1e-111
Identity = 203/333 (60.96%), Postives = 260/333 (78.08%), Query Frame = 1

Query: 6   KMVKISHMIGLFMAAMSLVVGAYDVLLGAYYGLLGDNLPSPSSVVQLCRKYNISRVRLEV 65
           K+V+I  ++   + A + VVGAYD+LLGAYYGL+GDNLP P  VVQLC +YNI RVRL+V
Sbjct: 3   KVVQIVFLMASILMAAT-VVGAYDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDV 62

Query: 66  PNLDVIEGFRGTQIDLSFGVPNDMLPNMASNKTL---WFNTYVEPYTNDCTINYIVVGDK 125
           P++DV+  FRG  ID+SFG+PN+ML ++A+NK L   WFNTYV+ + +D  INYI+VGDK
Sbjct: 63  PDVDVLTAFRGGAIDVSFGIPNNMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDK 122

Query: 126 AIPGLDDNILPVMKSLQDLLNARYFGAVKLTTLVGFNALAVT-NPPSSGAFNPVIVENMR 185
           AIP   + ILP+M SLQDLLNA Y G VKLTTLVG+NA  V+ +PPSSG F+P + E+MR
Sbjct: 123 AIPSHANFILPIMMSLQDLLNANYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMR 182

Query: 186 GILEFLRGQGSPLMVSVYTYDQYVYGGNISLGYATLSEQNTLVRDGMLSYNNLFDEMVDA 245
           GIL+FL  +GS LMVSV+ + +Y + G ISL YA  +E N +V+D  L Y NLFD MVDA
Sbjct: 183 GILKFLAEEGSALMVSVFPHQKYEFNGGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDA 242

Query: 246 FYAAMDKENVGDVAIVFGETGWPTCGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIY 305
           FYAA++KE VG+V IV GETGWPTCG+ +  T  +A E+N  FKSHISSG GTP+K N+Y
Sbjct: 243 FYAAIEKEMVGEVNIVVGETGWPTCGDISG-TQAIAKEYNNKFKSHISSGNGTPRKPNVY 302

Query: 306 IEGFIRSIFNENQKPEGESRCYGMFNVDSTPIY 335
           +EGFI+SIFNE++KPEG+S+CYGMF+V+  PIY
Sbjct: 303 LEGFIQSIFNEDKKPEGDSQCYGMFDVNMKPIY 333

BLAST of Cp4.1LG18g00200 vs. NCBI nr
Match: gi|778665333|ref|XP_004144115.2| (PREDICTED: lichenase-2-like [Cucumis sativus])

HSP 1 Score: 362.1 bits (928), Expect = 1.4e-96
Identity = 182/270 (67.41%), Postives = 214/270 (79.26%), Query Frame = 1

Query: 75  RGTQIDLSFGVPNDMLPNMASNKTL---WFNTYVEPYTNDCTINYIVVGDKAIPGLDDNI 134
           RGT I++SF VP +++ N A+N T    WF  YV P+  + TINYIVVGDK IPGLDDNI
Sbjct: 7   RGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLDDNI 66

Query: 135 LPVMKSLQDLLNARYFGAVKLTTLVGFNALAVTNPPSSGAFNPVIVENMRGILEFLRGQG 194
           LPVM+SLQDLLN+RY G VK+TT+VG  AL V +PPSSGAF+P ++ENM+GIL+FL GQG
Sbjct: 67  LPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENMKGILKFLWGQG 126

Query: 195 SPLMVSVYTYDQYVYGG---NISLGYATL---SEQNTLVR-DGMLSYNNLFDEMVDAFYA 254
           SPLM+S+Y YD Y Y G   NISLGYAT    +EQN  +R DG LSYNN+FDEMVDAFYA
Sbjct: 127 SPLMLSLYPYDAYAYTGYTNNISLGYATFTSQTEQNPPIRTDGDLSYNNIFDEMVDAFYA 186

Query: 255 AMDKENVGDVAIVFGETGWPTCGNYNATTPLLAAEFNRNFKSHISSGKGTPKKENIYIEG 314
           A+DK NVGDVAI  GETGWPT GNY A  P LA  +N NFK+HI+SGKGTP   NIYIEG
Sbjct: 187 AIDKANVGDVAIAIGETGWPTNGNYGA-GPSLALTYNLNFKNHITSGKGTPMMPNIYIEG 246

Query: 315 FIRSIFNENQKPEGESRCYGMFNVDSTPIY 335
           FIRS+FNEN+KPEGES+ YGMF++D TPIY
Sbjct: 247 FIRSLFNENEKPEGESQFYGMFHIDFTPIY 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
BG4_ARATH1.5e-6441.35Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana GN=BG4 PE=... [more]
BG5_ARATH4.3e-6440.77Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana GN=BG5 PE=... [more]
E13B_HORVU2.3e-4936.94Glucan endo-1,3-beta-glucosidase GII OS=Hordeum vulgare PE=1 SV=1[more]
GUB2_HORVU5.0e-4934.95Lichenase-2 (Fragment) OS=Hordeum vulgare PE=1 SV=1[more]
E13A_SOYBN1.5e-4835.91Glucan endo-1,3-beta-glucosidase OS=Glycine max PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LR03_CUCSA7.0e-11464.55Uncharacterized protein OS=Cucumis sativus GN=Csa_2G416760 PE=3 SV=1[more]
A0A0A0LZ10_CUCSA4.2e-11160.96Uncharacterized protein OS=Cucumis sativus GN=Csa_1G595900 PE=3 SV=1[more]
A0A0A0LWM4_CUCSA1.0e-9667.41Uncharacterized protein OS=Cucumis sativus GN=Csa_1G595910 PE=3 SV=1[more]
M5VWE6_PRUPE2.5e-7144.65Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa017802mg PE=3 SV=1[more]
M5VS03_PRUPE9.5e-7145.79Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa015919mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G20330.18.2e-6641.35 beta-1,3-glucanase 4[more]
AT5G20340.12.4e-6540.77 beta-1,3-glucanase 5[more]
AT5G20390.13.8e-6340.71 Glycosyl hydrolase superfamily protein[more]
AT5G20560.11.6e-6138.11 Glycosyl hydrolase superfamily protein[more]
AT1G33220.13.6e-6140.51 Glycosyl hydrolase superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659100571|ref|XP_008451158.1|3.8e-12168.39PREDICTED: uncharacterized protein LOC103492536 [Cucumis melo][more]
gi|659100567|ref|XP_008451156.1|8.2e-11661.54PREDICTED: putative glucan endo-1,3-beta-glucosidase GVI [Cucumis melo][more]
gi|778674443|ref|XP_004153772.2|1.0e-11364.55PREDICTED: lichenase-2-like [Cucumis sativus][more]
gi|778665329|ref|XP_004144124.2|6.1e-11160.96PREDICTED: putative glucan endo-1,3-beta-glucosidase GVI [Cucumis sativus][more]
gi|778665333|ref|XP_004144115.2|1.4e-9667.41PREDICTED: lichenase-2-like [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0004553hydrolase activity, hydrolyzing O-glycosyl compounds
Vocabulary: INTERPRO
TermDefinition
IPR017853Glycoside_hydrolase_SF
IPR013781Glycoside hydrolase, catalytic domain
IPR000490Glyco_hydro_17
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g00200.1Cp4.1LG18g00200.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 32..337
score: 6.6E-67coord: 338..454
score: 3.0
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 254..267
score: -coord: 372..385
scor
IPR013781Glycoside hydrolase, catalytic domainGENE3DG3DSA:3.20.20.80coord: 33..337
score: 3.4E-78coord: 339..454
score: 4.9
IPR017853Glycoside hydrolase superfamilyunknownSSF51445(Trans)glycosidasescoord: 32..336
score: 2.11E-58coord: 341..454
score: 1.11
NoneNo IPR availablePANTHERPTHR32227FAMILY NOT NAMEDcoord: 26..429
score: 2.2
NoneNo IPR availablePANTHERPTHR32227:SF103BETA-1,3-GLUCANASE 4 (PRECURSOR)-RELATEDcoord: 26..429
score: 2.2

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None