Cp4.1LG04g03950 (gene) Cucurbita pepo (Zucchini)

NameCp4.1LG04g03950
Typegene
OrganismCucurbita pepo (Cucurbita pepo (Zucchini))
DescriptionCalcium-binding EF-hand family protein
LocationCp4.1LG04 : 6924811 .. 6925224 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTGTGGAGATATTGGACAGTGCCACCATTGTCAACTTCGTCGAAGATGAGGCAGCCTTTGGTGCCTGCATAGGAGACCGGTTTGCTCACCTCGACACGAATCATGACGGTCTCCTTTCCTATAGTGAGATGTTGAAGGAGTTGCAGAGCCTCAGGGTCATCGAGGCCGATTTTGGAATCGACGTGAAGCCTGATCCAGATGAGCTTTCTTCTGTCTATAGTTCTATGTTTTTGCAGTTTGATCGTAACTCGAGTGGAACGGTTGATCTGGATGAGTTTAAGGCTGAGAACAAAAGGATGATGCTAGCTATGGCTAATGGGATTGGAGCTTTACCTGTTCAAATGGTGCTAGAAGAGGGCAGCTTCTTGATGAAGGCTGTTGAGAGAGAAGTTGCTATAATGACAACTTGA

mRNA sequence

ATGAGTGTGGAGATATTGGACAGTGCCACCATTGTCAACTTCGTCGAAGATGAGGCAGCCTTTGGTGCCTGCATAGGAGACCGGTTTGCTCACCTCGACACGAATCATGACGGTCTCCTTTCCTATAGTGAGATGTTGAAGGAGTTGCAGAGCCTCAGGGTCATCGAGGCCGATTTTGGAATCGACGTGAAGCCTGATCCAGATGAGCTTTCTTCTGTCTATAGTTCTATGTTTTTGCAGTTTGATCGTAACTCGAGTGGAACGGTTGATCTGGATGAGTTTAAGGCTGAGAACAAAAGGATGATGCTAGCTATGGCTAATGGGATTGGAGCTTTACCTGTTCAAATGGTGCTAGAAGAGGGCAGCTTCTTGATGAAGGCTGTTGAGAGAGAAGTTGCTATAATGACAACTTGA

Coding sequence (CDS)

ATGAGTGTGGAGATATTGGACAGTGCCACCATTGTCAACTTCGTCGAAGATGAGGCAGCCTTTGGTGCCTGCATAGGAGACCGGTTTGCTCACCTCGACACGAATCATGACGGTCTCCTTTCCTATAGTGAGATGTTGAAGGAGTTGCAGAGCCTCAGGGTCATCGAGGCCGATTTTGGAATCGACGTGAAGCCTGATCCAGATGAGCTTTCTTCTGTCTATAGTTCTATGTTTTTGCAGTTTGATCGTAACTCGAGTGGAACGGTTGATCTGGATGAGTTTAAGGCTGAGAACAAAAGGATGATGCTAGCTATGGCTAATGGGATTGGAGCTTTACCTGTTCAAATGGTGCTAGAAGAGGGCAGCTTCTTGATGAAGGCTGTTGAGAGAGAAGTTGCTATAATGACAACTTGA

Protein sequence

MSVEILDSATIVNFVEDEAAFGACIGDRFAHLDTNHDGLLSYSEMLKELQSLRVIEADFGIDVKPDPDELSSVYSSMFLQFDRNSSGTVDLDEFKAENKRMMLAMANGIGALPVQMVLEEGSFLMKAVEREVAIMTT
BLAST of Cp4.1LG04g03950 vs. TrEMBL
Match: A0A0A0KHT7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G418660 PE=4 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 2.0e-56
Identity = 116/134 (86.57%), Postives = 123/134 (91.79%), Query Frame = 1

Query: 1   MSVEILDSATIVNFVEDEAAFGACIGDRFAHLDTNHDGLLSYSEMLKELQSLRVIEADFG 60
           MSVEILDSATIVNFVED+ AFGA I DRFAHLDTN DGLLSYSEML+EL +LRV E  FG
Sbjct: 1   MSVEILDSATIVNFVEDDVAFGAFIRDRFAHLDTNRDGLLSYSEMLEELHTLRVFETHFG 60

Query: 61  IDVKPDPDELSSVYSSMFLQFDRNSSGTVDLDEFKAENKRMMLAMANGIGALPVQMVLEE 120
           IDVKPDPDELSSVYSS+FLQFDR+SSGTVDLDEF+AE KRMMLAMANG+G LPVQMVLEE
Sbjct: 61  IDVKPDPDELSSVYSSLFLQFDRDSSGTVDLDEFRAETKRMMLAMANGMGFLPVQMVLEE 120

Query: 121 GSFLMKAVEREVAI 135
           GSFLMKAVERE AI
Sbjct: 121 GSFLMKAVERETAI 134

BLAST of Cp4.1LG04g03950 vs. TrEMBL
Match: A0A0A0K3B2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037625 PE=4 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 9.6e-51
Identity = 109/135 (80.74%), Postives = 117/135 (86.67%), Query Frame = 1

Query: 1   MSVEILDSATIVNFVEDEAAFGACIGDRFAHLDTNHDGLLSYSEMLKELQSLRVIEADFG 60
           MSVEILD  TIVNFVEDEAAF A I  +FAHLDT+HDGLLSY+EML+ELQSLRV +ADFG
Sbjct: 1   MSVEILDGTTIVNFVEDEAAFNAYIRAQFAHLDTDHDGLLSYNEMLEELQSLRVFDADFG 60

Query: 61  IDVKPDPDELSSVYSSMFLQFDRNSSGTVDLDEFKAENKRMMLAMANGIGALPVQMVLEE 120
            D K  PDELSSVYSS+FLQFDR+S G VDLD +K E KRMMLAMANGIG LPVQMVLEE
Sbjct: 61  NDEKLGPDELSSVYSSLFLQFDRDSDGMVDLDAYKTEIKRMMLAMANGIGFLPVQMVLEE 120

Query: 121 GSFLMKAVEREVAIM 136
           GSFLMKAVERE AIM
Sbjct: 121 GSFLMKAVERETAIM 135

BLAST of Cp4.1LG04g03950 vs. TrEMBL
Match: A0A061DTS7_THECC (Calcium-binding EF-hand family protein OS=Theobroma cacao GN=TCM_002258 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 2.4e-49
Identity = 102/131 (77.86%), Postives = 116/131 (88.55%), Query Frame = 1

Query: 1   MSVEILDSATIVNFVEDEAAFGACIGDRFAHLDTNHDGLLSYSEMLKELQSLRVIEADFG 60
           MSVE+LDSATIVNFVEDE AF   I DRFAHLDT+HDGLLSY+E+LKELQSLRV E  FG
Sbjct: 1   MSVEVLDSATIVNFVEDEEAFNVWICDRFAHLDTDHDGLLSYAELLKELQSLRVFETHFG 60

Query: 61  IDVKPDPDELSSVYSSMFLQFDRNSSGTVDLDEFKAENKRMMLAMANGIGALPVQMVLEE 120
           IDVK DP EL+ VY+S+F+QFD +S+GTVDL EFKAE+KRMMLAMANG+G LPVQM+LEE
Sbjct: 61  IDVKTDPKELAHVYNSLFVQFDHDSNGTVDLGEFKAESKRMMLAMANGMGFLPVQMLLEE 120

Query: 121 GSFLMKAVERE 132
           GSFL KAV+RE
Sbjct: 121 GSFLKKAVDRE 131

BLAST of Cp4.1LG04g03950 vs. TrEMBL
Match: V4U9W3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10018069mg PE=4 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 1.2e-48
Identity = 99/131 (75.57%), Postives = 118/131 (90.08%), Query Frame = 1

Query: 1   MSVEILDSATIVNFVEDEAAFGACIGDRFAHLDTNHDGLLSYSEMLKELQSLRVIEADFG 60
           MSVE+LDSATIV+FVEDE AF   I +RFAHLDT+HDGLLSY+EM++EL+SLRV E  FG
Sbjct: 1   MSVEVLDSATIVSFVEDEEAFNNSIRNRFAHLDTDHDGLLSYAEMMQELKSLRVFETHFG 60

Query: 61  IDVKPDPDELSSVYSSMFLQFDRNSSGTVDLDEFKAENKRMMLAMANGIGALPVQMVLEE 120
           ID KPDP+EL+SVY+S+F+QFD +S+GTVDL+EFKAE K+MMLAMANG+G LPVQMVLEE
Sbjct: 61  IDEKPDPEELASVYNSLFVQFDHDSNGTVDLEEFKAETKQMMLAMANGLGFLPVQMVLEE 120

Query: 121 GSFLMKAVERE 132
            SFLMKAVE+E
Sbjct: 121 DSFLMKAVEKE 131

BLAST of Cp4.1LG04g03950 vs. TrEMBL
Match: A0A0D2M0N6_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_001G194900 PE=4 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 7.6e-48
Identity = 100/131 (76.34%), Postives = 113/131 (86.26%), Query Frame = 1

Query: 1   MSVEILDSATIVNFVEDEAAFGACIGDRFAHLDTNHDGLLSYSEMLKELQSLRVIEADFG 60
           MSVE+LDS TIVNFVEDE AF   I DRFAHLD +HDGLLSYSE+LKELQSLRV+E  FG
Sbjct: 1   MSVELLDSGTIVNFVEDEEAFHVSIRDRFAHLDADHDGLLSYSELLKELQSLRVLETHFG 60

Query: 61  IDVKPDPDELSSVYSSMFLQFDRNSSGTVDLDEFKAENKRMMLAMANGIGALPVQMVLEE 120
           IDV+ DP EL+ VY S+F+QFD +S+GTVDL+EFK+E KRMMLAMANG+G LPVQMVLEE
Sbjct: 61  IDVQTDPKELAHVYKSLFVQFDHDSNGTVDLEEFKSETKRMMLAMANGMGFLPVQMVLEE 120

Query: 121 GSFLMKAVERE 132
           GSFL  AVERE
Sbjct: 121 GSFLKIAVERE 131

BLAST of Cp4.1LG04g03950 vs. TAIR10
Match: AT2G44310.1 (AT2G44310.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 125.9 bits (315), Expect = 1.9e-29
Identity = 60/135 (44.44%), Postives = 99/135 (73.33%), Query Frame = 1

Query: 1   MSVEILDSATIVNFVEDEAAFGACIGDRFAHLDTNHDGLLSYSEMLKELQSLRVIEADFG 60
           M V ++D +T+ +FV+DE  F   + +RFA LD N DG+LS SE+ K  +S+R++E+ FG
Sbjct: 1   MGVVLIDGSTVRSFVDDEEQFKKSVDERFAALDLNKDGVLSRSELRKAFESMRLLESHFG 60

Query: 61  IDVKPDPDELSSVYSSMFLQFDRNSSGTVDLDEFKAENKRMMLAMANGIGALPVQMVL-- 120
           +DV    DEL+++Y S+F +FD + SG+VDL+EF++E K+++LA+A+G+G+ P+ MVL  
Sbjct: 61  VDVVTPQDELTNLYDSIFEKFDTDQSGSVDLEEFRSEMKKIVLAIADGLGSCPITMVLDD 120

Query: 121 EEGSFLMKAVEREVA 134
           ++ +FL KA + E +
Sbjct: 121 DDDNFLKKAADLEAS 135

BLAST of Cp4.1LG04g03950 vs. NCBI nr
Match: gi|778716395|ref|XP_011657544.1| (PREDICTED: uncharacterized protein LOC105435869 [Cucumis sativus])

HSP 1 Score: 226.5 bits (576), Expect = 2.9e-56
Identity = 116/134 (86.57%), Postives = 123/134 (91.79%), Query Frame = 1

Query: 1   MSVEILDSATIVNFVEDEAAFGACIGDRFAHLDTNHDGLLSYSEMLKELQSLRVIEADFG 60
           MSVEILDSATIVNFVED+ AFGA I DRFAHLDTN DGLLSYSEML+EL +LRV E  FG
Sbjct: 1   MSVEILDSATIVNFVEDDVAFGAFIRDRFAHLDTNRDGLLSYSEMLEELHTLRVFETHFG 60

Query: 61  IDVKPDPDELSSVYSSMFLQFDRNSSGTVDLDEFKAENKRMMLAMANGIGALPVQMVLEE 120
           IDVKPDPDELSSVYSS+FLQFDR+SSGTVDLDEF+AE KRMMLAMANG+G LPVQMVLEE
Sbjct: 61  IDVKPDPDELSSVYSSLFLQFDRDSSGTVDLDEFRAETKRMMLAMANGMGFLPVQMVLEE 120

Query: 121 GSFLMKAVEREVAI 135
           GSFLMKAVERE AI
Sbjct: 121 GSFLMKAVERETAI 134

BLAST of Cp4.1LG04g03950 vs. NCBI nr
Match: gi|659097011|ref|XP_008449396.1| (PREDICTED: uncharacterized protein LOC103491294 [Cucumis melo])

HSP 1 Score: 224.9 bits (572), Expect = 8.3e-56
Identity = 115/134 (85.82%), Postives = 122/134 (91.04%), Query Frame = 1

Query: 1   MSVEILDSATIVNFVEDEAAFGACIGDRFAHLDTNHDGLLSYSEMLKELQSLRVIEADFG 60
           MSVEILDSATIVNFVED+ AFGA I DRFAHLDTNHDGLLSYSEML+EL +LRV E  FG
Sbjct: 1   MSVEILDSATIVNFVEDDVAFGAFIRDRFAHLDTNHDGLLSYSEMLEELHTLRVFETHFG 60

Query: 61  IDVKPDPDELSSVYSSMFLQFDRNSSGTVDLDEFKAENKRMMLAMANGIGALPVQMVLEE 120
           IDVKPDP+ELSSVYSS+FLQFD +SSGTVDLDEFKAE KRMML MANG+G LPVQMVLEE
Sbjct: 61  IDVKPDPEELSSVYSSLFLQFDHDSSGTVDLDEFKAETKRMMLDMANGMGFLPVQMVLEE 120

Query: 121 GSFLMKAVEREVAI 135
           GSFLMKAVERE AI
Sbjct: 121 GSFLMKAVERETAI 134

BLAST of Cp4.1LG04g03950 vs. NCBI nr
Match: gi|659110959|ref|XP_008455501.1| (PREDICTED: uncharacterized protein LOC103495653 [Cucumis melo])

HSP 1 Score: 218.0 bits (554), Expect = 1.0e-53
Identity = 114/135 (84.44%), Postives = 119/135 (88.15%), Query Frame = 1

Query: 1   MSVEILDSATIVNFVEDEAAFGACIGDRFAHLDTNHDGLLSYSEMLKELQSLRVIEADFG 60
           MSVEILDS TIVNFVEDEAAF A I DRF HLDTNHDGLLSY+EML+ELQSLRV EADFG
Sbjct: 1   MSVEILDSTTIVNFVEDEAAFNAYIRDRFTHLDTNHDGLLSYNEMLEELQSLRVFEADFG 60

Query: 61  IDVKPDPDELSSVYSSMFLQFDRNSSGTVDLDEFKAENKRMMLAMANGIGALPVQMVLEE 120
            D K DPDELSSVYSS+FLQFDR+S G VDLD +K E KRMMLAMANGIG LPVQMVLEE
Sbjct: 61  NDKKLDPDELSSVYSSLFLQFDRDSDGMVDLDAYKTEIKRMMLAMANGIGFLPVQMVLEE 120

Query: 121 GSFLMKAVEREVAIM 136
           GSFLMKAVERE AIM
Sbjct: 121 GSFLMKAVERETAIM 135

BLAST of Cp4.1LG04g03950 vs. NCBI nr
Match: gi|778723608|ref|XP_011658679.1| (PREDICTED: uncharacterized protein LOC105436072 [Cucumis sativus])

HSP 1 Score: 207.6 bits (527), Expect = 1.4e-50
Identity = 109/135 (80.74%), Postives = 117/135 (86.67%), Query Frame = 1

Query: 1   MSVEILDSATIVNFVEDEAAFGACIGDRFAHLDTNHDGLLSYSEMLKELQSLRVIEADFG 60
           MSVEILD  TIVNFVEDEAAF A I  +FAHLDT+HDGLLSY+EML+ELQSLRV +ADFG
Sbjct: 1   MSVEILDGTTIVNFVEDEAAFNAYIRAQFAHLDTDHDGLLSYNEMLEELQSLRVFDADFG 60

Query: 61  IDVKPDPDELSSVYSSMFLQFDRNSSGTVDLDEFKAENKRMMLAMANGIGALPVQMVLEE 120
            D K  PDELSSVYSS+FLQFDR+S G VDLD +K E KRMMLAMANGIG LPVQMVLEE
Sbjct: 61  NDEKLGPDELSSVYSSLFLQFDRDSDGMVDLDAYKTEIKRMMLAMANGIGFLPVQMVLEE 120

Query: 121 GSFLMKAVEREVAIM 136
           GSFLMKAVERE AIM
Sbjct: 121 GSFLMKAVERETAIM 135

BLAST of Cp4.1LG04g03950 vs. NCBI nr
Match: gi|590711914|ref|XP_007049241.1| (Calcium-binding EF-hand family protein [Theobroma cacao])

HSP 1 Score: 203.0 bits (515), Expect = 3.4e-49
Identity = 102/131 (77.86%), Postives = 116/131 (88.55%), Query Frame = 1

Query: 1   MSVEILDSATIVNFVEDEAAFGACIGDRFAHLDTNHDGLLSYSEMLKELQSLRVIEADFG 60
           MSVE+LDSATIVNFVEDE AF   I DRFAHLDT+HDGLLSY+E+LKELQSLRV E  FG
Sbjct: 1   MSVEVLDSATIVNFVEDEEAFNVWICDRFAHLDTDHDGLLSYAELLKELQSLRVFETHFG 60

Query: 61  IDVKPDPDELSSVYSSMFLQFDRNSSGTVDLDEFKAENKRMMLAMANGIGALPVQMVLEE 120
           IDVK DP EL+ VY+S+F+QFD +S+GTVDL EFKAE+KRMMLAMANG+G LPVQM+LEE
Sbjct: 61  IDVKTDPKELAHVYNSLFVQFDHDSNGTVDLGEFKAESKRMMLAMANGMGFLPVQMLLEE 120

Query: 121 GSFLMKAVERE 132
           GSFL KAV+RE
Sbjct: 121 GSFLKKAVDRE 131

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0KHT7_CUCSA2.0e-5686.57Uncharacterized protein OS=Cucumis sativus GN=Csa_6G418660 PE=4 SV=1[more]
A0A0A0K3B2_CUCSA9.6e-5180.74Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037625 PE=4 SV=1[more]
A0A061DTS7_THECC2.4e-4977.86Calcium-binding EF-hand family protein OS=Theobroma cacao GN=TCM_002258 PE=4 SV=... [more]
V4U9W3_9ROSI1.2e-4875.57Uncharacterized protein OS=Citrus clementina GN=CICLE_v10018069mg PE=4 SV=1[more]
A0A0D2M0N6_GOSRA7.6e-4876.34Uncharacterized protein OS=Gossypium raimondii GN=B456_001G194900 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G44310.11.9e-2944.44 Calcium-binding EF-hand family protein[more]
Match NameE-valueIdentityDescription
gi|778716395|ref|XP_011657544.1|2.9e-5686.57PREDICTED: uncharacterized protein LOC105435869 [Cucumis sativus][more]
gi|659097011|ref|XP_008449396.1|8.3e-5685.82PREDICTED: uncharacterized protein LOC103491294 [Cucumis melo][more]
gi|659110959|ref|XP_008455501.1|1.0e-5384.44PREDICTED: uncharacterized protein LOC103495653 [Cucumis melo][more]
gi|778723608|ref|XP_011658679.1|1.4e-5080.74PREDICTED: uncharacterized protein LOC105436072 [Cucumis sativus][more]
gi|590711914|ref|XP_007049241.1|3.4e-4977.86Calcium-binding EF-hand family protein [Theobroma cacao][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR018247EF_Hand_1_Ca_BS
IPR011992EF-hand-dom_pair
IPR002048EF_hand_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG04g03950.1Cp4.1LG04g03950.1mRNA


Analysis Name: InterPro Annotations of Cucurbita pepo
Date Performed: 2017-12-02
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 26..95
score: 4.5
IPR002048EF-hand domainSMARTSM00054efh_1coord: 24..52
score: 2.2coord: 73..101
score:
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 20..55
score: 7.651coord: 69..104
score:
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 29..96
score: 1.6
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 27..96
score: 2.24
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 33..45
score: -coord: 82..94
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 1..135
score: 5.7
NoneNo IPR availablePANTHERPTHR10891:SF615SUBFAMILY NOT NAMEDcoord: 1..135
score: 5.7

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None