Lsi01G020770 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi01G020770
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Major facilitator superfamily protein
Location	chr01 : 29277894 .. 29279793 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGAGGCAGCGGCCGCGGGTTTCTTGCACAAGCTGTTAACCGGACGGTGGTTAATGCTATTCGCATCACTAACCATCATGGCAATGAACGGATCAGGCTACATGTTCGGGCTCTACTCCGCCCGCATCAAATCCATTTTTCGTTACGATCAGTCGGCTCTTAATTTTATCAGCTTCTTCAAGGACTTGGGTGGCAATCTTGGGGTGGTTTCCGGCCTCCTCTACGAGGTCGCACCACCGTGGCTGGTTCTCTCTGTTGGTGCCGTCCTCAACTTTTTCGCCTACTTCATGCTCTGGTCGGCGGTTTCTGGCCGGACCGCCGCACCGGGGCTGTGGTTGATGTGTGTTTATATGGGCGTTGCTGCTAACTCATTGTCTTTTGCTAATACGGCGGCTTTGGTTACTTGTGTTCGTAACTTTCCTCTTCATCGTGGCTGCCTTCTTAAAGGGTATATATACTCAACTGTCAACTCATCATCATTTTTTGAGAGAGGATTATTATTCTAATCTCTTTCACTTATTGTTTTGTTTATTAATCTTGAAAATCATGATCTTCTTAATCATTGGTTGAATGGGGAATGGGAGGGTTTGGGCATTTCGAAATAATTTCATATTCGAAATGTTTTGCAATTCGTTGATAACTTTTAATTTTGTGTTAAATATTTAATAAATTCATGAAGATGAGTCTCAATTTTTTCAATTTGAAATAAAATTTAATTTTATATCTAATTGAATCATAAGTTTTAAATTTTATACCTAATAGAAGATATATGGATTTTAATGAATGTTGAATAGGTTAATTATTAGGCACAAAATTAAAATTTATGTCAAATGGAACATGTTGAACTTTTAAGATTTTCAAATATGTCAAAGACTATTGAAACAAGAAATTGAAAGTACAAAAATTTAATAAAAATAAAATTTTAAATTTTAGGTCTCCTTTGGTAACTATTTGGTTTTTTGTTTTTTGTTTTTGAAAATTAAGCCTAAAAACACTTCTTTCACCTCTAATTTCTTGATTTGGTGTCTACTTGTAACTAATGTTTTCAAAAACCAAGACAAATTTTAAAAACTAAAAATATAGTTTTAAAAAACTTTTTTTTTTTTTGAATTTGACTAAGAATTCAAATCTTGTATTTAAGAAAGATACAAATCGTTATAAGAAATTGAGAGAAAATAGGCTTTAATTTAAAAAACGAAACGATTATTAAACGGGATTTTGAAGACACATTGGACACTTCTTAGGGGGTGTTTGGAATAAAGAGTGGAATAGTGAGGAGTAAGATGTTACGAAGTCTTAGGGAGTGAACTCTTGGAGGCCCACAATATAAAGAGTTGATAAGATATAAAATTAATGTTTTCTAGTAGTGAGACCCACCAACTTCTTAGACCAAATACGGAGTAGAATTCATGAACTTGTACTTTCTATCTCCATGGGTAAACACACCCTTAAAGTTTACAAATCATATATATATAAATCTAAAATTTCAAAGACTAAACTTATAATTAAATTATATATAAATTTGTGTCCCTTAGACGTAAAGTTTTAAATCTACCTCTATGAAATGAAAGTTTAAGAACACATATTATATGCAATAGACGAAAATGAAGTTTAAAGACTAAATTTGTAATTTAATATAGATACATAGGTTTGAGTGGTGCAATAATGACGCAACTTTACCATGCCATGTATGGAGATGACGAACCAGAAGGCCTTATTTTGATGATTGCTTGGCTCCCATCTGCCATTTCATTAGCTTCCCTTCCATTCATTCGAATCATAAACACCATCAACCATCAACCACCAAGCCAACAACCTCAAACCATTCTATACCCTTCTTTACATATCCCTCGCCCTTGCCACTTCTCTCATTGCCATTACCATCCCTCAAACTAA

mRNA sequence

ATGGCGGAGGCAGCGGCCGCGGGTTTCTTGCACAAGCTGTTAACCGGACGGTGGTTAATGCTATTCGCATCACTAACCATCATGGCAATGAACGGATCAGGCTACATGTTCGGGCTCTACTCCGCCCGCATCAAATCCATTTTTCGTTACGATCAGTCGGCTCTTAATTTTATCAGCTTCTTCAAGGACTTGGGTGGCAATCTTGGGGTGGTTTCCGGCCTCCTCTACGAGGTCGCACCACCGTGGCTGGTTCTCTCTGTTGGTGCCGTCCTCAACTTTTTCGCCTACTTCATGCTCTGGTCGGCGGTTTCTGGCCGGACCGCCGCACCGGGGCTGTGGTTGATGTGTGTTTATATGGGCGTTGCTGCTAACTCATTGTCTTTTGCTAATACGGCGGCTTTGGTTACTTGTGTTCGTAACTTTCCTCTTCATCGTGGCTGCCTTCTTAAAGGATACATAGGTTTGAGTGGTGCAATAATGACGCAACTTTACCATGCCATGTATGGAGATGACGAACCAGAAGGCCTTATTTTGATGATTGCTTGGCTCCCATCTGCCATTTCATTAGCTTCCCTTCCATTCATTCGAATCATAAACACCATCAACCATCAACCACCAAGCCAACAACCTCAAACCATTCTATACCCTTCTTTACATATCCCTCGCCCTTGCCACTTCTCTCATTGCCATTACCATCCCTCAAACTAA

Coding sequence (CDS)

ATGGCGGAGGCAGCGGCCGCGGGTTTCTTGCACAAGCTGTTAACCGGACGGTGGTTAATGCTATTCGCATCACTAACCATCATGGCAATGAACGGATCAGGCTACATGTTCGGGCTCTACTCCGCCCGCATCAAATCCATTTTTCGTTACGATCAGTCGGCTCTTAATTTTATCAGCTTCTTCAAGGACTTGGGTGGCAATCTTGGGGTGGTTTCCGGCCTCCTCTACGAGGTCGCACCACCGTGGCTGGTTCTCTCTGTTGGTGCCGTCCTCAACTTTTTCGCCTACTTCATGCTCTGGTCGGCGGTTTCTGGCCGGACCGCCGCACCGGGGCTGTGGTTGATGTGTGTTTATATGGGCGTTGCTGCTAACTCATTGTCTTTTGCTAATACGGCGGCTTTGGTTACTTGTGTTCGTAACTTTCCTCTTCATCGTGGCTGCCTTCTTAAAGGATACATAGGTTTGAGTGGTGCAATAATGACGCAACTTTACCATGCCATGTATGGAGATGACGAACCAGAAGGCCTTATTTTGATGATTGCTTGGCTCCCATCTGCCATTTCATTAGCTTCCCTTCCATTCATTCGAATCATAAACACCATCAACCATCAACCACCAAGCCAACAACCTCAAACCATTCTATACCCTTCTTTACATATCCCTCGCCCTTGCCACTTCTCTCATTGCCATTACCATCCCTCAAACTAA

Protein sequence

MAEAAAAGFLHKLLTGRWLMLFASLTIMAMNGSGYMFGLYSARIKSIFRYDQSALNFISFFKDLGGNLGVVSGLLYEVAPPWLVLSVGAVLNFFAYFMLWSAVSGRTAAPGLWLMCVYMGVAANSLSFANTAALVTCVRNFPLHRGCLLKGYIGLSGAIMTQLYHAMYGDDEPEGLILMIAWLPSAISLASLPFIRIINTINHQPPSQQPQTILYPSLHIPRPCHFSHCHYHPSN

Homology

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BLAST of Lsi01G020770 vs. TrEMBL
Match: A0A0A0L178_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G507390 PE=4 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 3.2e-94
Identity = 184/221 (83.26%), Postives = 193/221 (87.33%), Query Frame = 1


Query: 2   AEAAAAGFLHKLLTGRWLMLFASLTIMAMNGSGYMFGLYSARIKSIFRYDQSALNFISFF 61
           A AA  GFLHKLLTGRWLMLFASL IMAMNGSGYMFGLYS  IKS+F YDQS LN ISFF
Sbjct: 5   ALAAVTGFLHKLLTGRWLMLFASLIIMAMNGSGYMFGLYSNHIKSVFGYDQSTLNLISFF 64

Query: 62  KDLGGNLGVVSGLLYEVAPPWLVLSVGAVLNFFAYFMLWSAVSGRTAAPGLWLMCVYMGV 121
           KDLG NLGVVSGLLYEVAPPWLVLSVGA+LNFF YFMLW AVSGRTAAPGL LMCVYM V
Sbjct: 65  KDLGANLGVVSGLLYEVAPPWLVLSVGAILNFFGYFMLWLAVSGRTAAPGLRLMCVYMSV 124

Query: 122 AANSLSFANTAALVTCVRNFPLHRGC---LLKGYIGLSGAIMTQLYHAMYGDDEPEGLIL 181
           AANSLSF NTAALVTC+RNFPLHRGC   LLKGYIGLSGAIMTQLYHAMYG+D PEGLIL
Sbjct: 125 AANSLSFGNTAALVTCLRNFPLHRGCLLGLLKGYIGLSGAIMTQLYHAMYGEDNPEGLIL 184

Query: 182 MIAWLPSAISLASLPFIRIINTINHQPPSQQP-QTILYPSL 219
           MIAWLPSAISLASLPFIR+IN+ N+Q    +P   +LY SL
Sbjct: 185 MIAWLPSAISLASLPFIRLINSNNNQRNDLKPFYNLLYISL 225

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BLAST of Lsi01G020770 vs. TrEMBL
Match: A0A151TVG0_CAJCA (Putative transporter MCH1 OS=Cajanus cajan GN=KK1_010259 PE=4 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 9.6e-67
Identity = 129/216 (59.72%), Postives = 163/216 (75.46%), Query Frame = 1


Query: 8   GFLHKLLTGRWLMLFASLTIMAMNGSGYMFGLYSARIKSIFRYDQSALNFISFFKDLGGN 67
           GF + +LTGRW MLFASL IM++ G+ YMFG+YS  +K+   YDQS LN + FFKDLG N
Sbjct: 16  GFSYHVLTGRWFMLFASLLIMSVAGATYMFGIYSNEVKTSLGYDQSTLNLLGFFKDLGAN 75

Query: 68  LGVVSGLLYEVAPPWLVLSVGAVLNFFAYFMLWSAVSGRTAAPGLWLMCVYMGVAANSLS 127
           +GV+SGL+ E+ PPW+VLS+G ++NFF YFM+W AVSGR A P +W MC+Y+ + ANS +
Sbjct: 76  VGVISGLVNEITPPWVVLSIGVIMNFFGYFMIWLAVSGRIAKPQVWQMCLYICIGANSQT 135

Query: 128 FANTAALVTCVRNFPLHRGC---LLKGYIGLSGAIMTQLYHAMYGDDEPEGLILMIAWLP 187
           FANT ALVTCV+NFP  RG    LLKGY+GLSGAI+ QLYHA YGD  P+ LIL+IAWLP
Sbjct: 136 FANTGALVTCVKNFPGSRGSILGLLKGYVGLSGAIIAQLYHAFYGDHNPQALILLIAWLP 195

Query: 188 SAISLASLPFIRIINTINHQPPSQQPQTILYPSLHI 221
           +A+S   LP IRI+NTI HQP   +   + Y  L+I
Sbjct: 196 AAVSFLFLPTIRIMNTI-HQPKDNR---VFYHLLYI 227

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BLAST of Lsi01G020770 vs. TrEMBL
Match: A0A0S3RYX4_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.04G333600 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.1e-66
Identity = 129/213 (60.56%), Postives = 162/213 (76.06%), Query Frame = 1


Query: 11  HKLLTGRWLMLFASLTIMAMNGSGYMFGLYSARIKSIFRYDQSALNFISFFKDLGGNLGV 70
           + ++TGRW MLFASL IM++ G+ YMFG YS  +K+   YDQS LN +SFFKDLG N+GV
Sbjct: 14  YNVITGRWFMLFASLLIMSVAGATYMFGAYSNEVKTSLGYDQSTLNLLSFFKDLGANVGV 73

Query: 71  VSGLLYEVAPPWLVLSVGAVLNFFAYFMLWSAVSGRTAAPGLWLMCVYMGVAANSLSFAN 130
           VSGL+ E+ PPW+VLS+G ++NFF YFM+W AVSGR A P LW MC+Y+ + ANS +FAN
Sbjct: 74  VSGLVNEITPPWVVLSIGVLMNFFGYFMIWLAVSGRIAKPQLWKMCLYICIGANSQTFAN 133

Query: 131 TAALVTCVRNFPLHRGC---LLKGYIGLSGAIMTQLYHAMYGDDEPEGLILMIAWLPSAI 190
           T ALVTCV+NFP  RG    LLKGY+GLSGAI+TQLYHA YGD  P+ LIL+IAWLP+A+
Sbjct: 134 TGALVTCVKNFPGSRGSLLGLLKGYVGLSGAIITQLYHAFYGDHNPQALILLIAWLPAAV 193

Query: 191 SLASLPFIRIINTINHQPPSQQPQTILYPSLHI 221
           S   LP IRI NT+ HQP + +   + Y  L+I
Sbjct: 194 SFLFLPTIRIFNTV-HQPKNNR---VFYHLLYI 222

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BLAST of Lsi01G020770 vs. TrEMBL
Match: A0A0L9TFQ4_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan641s007000 PE=4 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.1e-66
Identity = 129/213 (60.56%), Postives = 162/213 (76.06%), Query Frame = 1


Query: 11  HKLLTGRWLMLFASLTIMAMNGSGYMFGLYSARIKSIFRYDQSALNFISFFKDLGGNLGV 70
           + ++TGRW MLFASL IM++ G+ YMFG YS  +K+   YDQS LN +SFFKDLG N+GV
Sbjct: 14  YNVITGRWFMLFASLLIMSVAGATYMFGAYSNEVKTSLGYDQSTLNLLSFFKDLGANVGV 73

Query: 71  VSGLLYEVAPPWLVLSVGAVLNFFAYFMLWSAVSGRTAAPGLWLMCVYMGVAANSLSFAN 130
           VSGL+ E+ PPW+VLS+G ++NFF YFM+W AVSGR A P LW MC+Y+ + ANS +FAN
Sbjct: 74  VSGLVNEITPPWVVLSIGVLMNFFGYFMIWLAVSGRIAKPQLWKMCLYICIGANSQTFAN 133

Query: 131 TAALVTCVRNFPLHRGC---LLKGYIGLSGAIMTQLYHAMYGDDEPEGLILMIAWLPSAI 190
           T ALVTCV+NFP  RG    LLKGY+GLSGAI+TQLYHA YGD  P+ LIL+IAWLP+A+
Sbjct: 134 TGALVTCVKNFPGSRGSLLGLLKGYVGLSGAIITQLYHAFYGDHNPQALILLIAWLPAAV 193

Query: 191 SLASLPFIRIINTINHQPPSQQPQTILYPSLHI 221
           S   LP IRI NT+ HQP + +   + Y  L+I
Sbjct: 194 SFLFLPTIRIFNTV-HQPKNNR---VFYHLLYI 222

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BLAST of Lsi01G020770 vs. TrEMBL
Match: V7D0U4_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_001G180500g PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.4e-65
Identity = 126/213 (59.15%), Postives = 162/213 (76.06%), Query Frame = 1


Query: 11  HKLLTGRWLMLFASLTIMAMNGSGYMFGLYSARIKSIFRYDQSALNFISFFKDLGGNLGV 70
           + ++TGRW MLFASL IM++ G+ YMF +YS  +K+   YDQS LN +SFFKDLG N+GV
Sbjct: 6   YHVITGRWFMLFASLLIMSVAGATYMFAIYSNEVKTSLGYDQSTLNLLSFFKDLGANVGV 65

Query: 71  VSGLLYEVAPPWLVLSVGAVLNFFAYFMLWSAVSGRTAAPGLWLMCVYMGVAANSLSFAN 130
           +SGL+ E+ PPW+VLS+G ++NFF YFM+W AVSGR A P +W MC+Y+ + ANS +FAN
Sbjct: 66  ISGLVNEITPPWVVLSIGVLMNFFGYFMIWLAVSGRIAKPQVWQMCLYICIGANSQTFAN 125

Query: 131 TAALVTCVRNFPLHRGC---LLKGYIGLSGAIMTQLYHAMYGDDEPEGLILMIAWLPSAI 190
           T ALVTCV+NFP  RG    LLKGY+GLSGAI+TQLYHA YGD  P+ LIL+IAWLP+A+
Sbjct: 126 TGALVTCVKNFPESRGSLLGLLKGYVGLSGAIITQLYHAFYGDHNPQALILLIAWLPAAV 185

Query: 191 SLASLPFIRIINTINHQPPSQQPQTILYPSLHI 221
           S   LP IRI NT+ HQP + +   + Y  L+I
Sbjct: 186 SFLFLPTIRIFNTV-HQPKNNR---VFYHLLYI 214

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BLAST of Lsi01G020770 vs. TAIR10
Match: AT2G39210.1 (AT2G39210.1 Major facilitator superfamily protein)

HSP 1 Score: 228.4 bits (581), Expect = 4.6e-60
Identity = 118/210 (56.19%), Postives = 147/210 (70.00%), Query Frame = 1


Query: 12  KLLTGRWLMLFASLTIMAMNGSGYMFGLYSARIKSIFRYDQSALNFISFFKDLGGNLGVV 71
           ++LTGRW M F SL IM+  G+ YMFG+YS  IK    YDQ+ LN +SFFKDLG N+GV+
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 72  SGLLYEVAPPWLVLSVGAVLNFFAYFMLWSAVSGRTAAPGLWLMCVYMGVAANSLSFANT 131
           +GLL EV PPW +L +GA+LNFF YFM+W AV+ R + P +W MC+Y+ V ANS SFANT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 132 AALVTCVRNFPLHRGC---LLKGYIGLSGAIMTQLYHAMYGDDEPEGLILMIAWLPSAIS 191
            +LVTCV+NFP  RG    +LKGY+GLSGAI+TQLY A YG+D  E LILMI WLP+ +S
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKE-LILMIGWLPAIVS 195

Query: 192 LASLPFIRIINTINHQPPSQQPQTILYPSL 219
            A L  IRI+         +     LY SL
Sbjct: 196 FAFLRTIRIMKVKRQTNELKVFYNFLYISL 224

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BLAST of Lsi01G020770 vs. TAIR10
Match: AT2G28120.1 (AT2G28120.1 Major facilitator superfamily protein)

HSP 1 Score: 214.9 bits (546), Expect = 5.2e-56
Identity = 117/239 (48.95%), Postives = 150/239 (62.76%), Query Frame = 1


Query: 9   FLHKLLTGRWLMLFASLTIMAMNGSGYMFGLYSARIKSIFRYDQSALNFISFFKDLGGNL 68
           FL     GRW M+FAS  IMA  G+ Y+FG YS  IKS   YDQ+ LN + FFKDLG N+
Sbjct: 9   FLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANV 68

Query: 69  GVVSGLLYEVAPPWLVLSVGAVLNFFAYFMLWSAVSGRTAAPGLWLMCVYMGVAANSLSF 128
           GV+SGL+ EV P W VL++G+ +NF  YFM+W  V+G+ A P +W MC+Y+ + ANS +F
Sbjct: 69  GVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNF 128

Query: 129 ANTAALVTCVRNFPLHRGC---LLKGYIGLSGAIMTQLYHAMYGDDEPEGLILMIAWLPS 188
           ANT ALVTCV+NFP  RG    LLKGY+GLSGAI TQLY A+YG D  + LIL+IAWLP+
Sbjct: 129 ANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDS-KSLILLIAWLPA 188

Query: 189 AISLASLPFIRIINTINHQPPSQQPQTILYPSLHI----------PRPCHFSHCHYHPS 235
           A+SL  +  IR    +  +         LY S+ +           +  HFS   Y  S
Sbjct: 189 AVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAAS 246

[Close]

BLAST of Lsi01G020770 vs. TAIR10
Match: AT1G18940.1 (AT1G18940.1 Nodulin-like / Major Facilitator Superfamily protein)

HSP 1 Score: 158.7 bits (400), Expect = 4.4e-39
Identity = 83/206 (40.29%), Postives = 132/206 (64.08%), Query Frame = 1


Query: 12  KLLTGRWLMLFASLTIMAMNGSGYMFGLYSARIKSIFRYDQSALNFISFFKDLGGNLGVV 71
           ++L  +W+ + AS+ I    G  Y FG+YSA +KS   YDQS L+ +S FKD+GGN+GV+
Sbjct: 4   EILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVL 63

Query: 72  SGLLYEVAP--------------PWLVLSVGAVLNFFAYFMLWSAVSGRTAAPGLWLMCV 131
           SGL+Y  A               PW+V+ +GA+LNF  YF++W++V+G    P + +MC+
Sbjct: 64  SGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCL 123

Query: 132 YMGVAANSLSFANTAALVTCVRNFPLHRGC---LLKGYIGLSGAIMTQLYHAMYGDDEPE 191
           +M +AA SL+F NTA +V+ + NF  + G    ++KG++GLSGA++ QLY  +   D P+
Sbjct: 124 FMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGD-PK 183

Query: 192 GLILMIAWLPSAISLASLPFIRIINT 201
             IL++A +PS +S+  +P +R+  T
Sbjct: 184 TFILLLAIVPSLLSVLVMPLVRVYKT 208

[Close]

BLAST of Lsi01G020770 vs. TAIR10
Match: AT1G74780.1 (AT1G74780.1 Nodulin-like / Major Facilitator Superfamily protein)

HSP 1 Score: 153.7 bits (387), Expect = 1.4e-37
Identity = 86/208 (41.35%), Postives = 127/208 (61.06%), Query Frame = 1


Query: 12  KLLTGRWLMLFASLTIMAMNGSGYMFGLYSARIKSIFRYDQSALNFISFFKDLGGNLGVV 71
           ++L  +W+ + AS+ I   +G+ Y FG+YSA +KS   YDQS L+ +S FKD+G N GV 
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 72  SGLLYEVAP----------------PWLVLSVGAVLNFFAYFMLWSAVSGRTAAPGLWLM 131
           SGLLY  A                 PW+VL+VGA+  F  YF++W++V+G    P + LM
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 132 CVYMGVAANSLSFANTAALVTCVRNFPLHRGC---LLKGYIGLSGAIMTQLYHAMYGDDE 191
           C++M +AA S +F NTA +V+ V NF  + G    ++KG++GLSGAI+ QLY  +   D 
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD- 181

Query: 192 PEGLILMIAWLPSAISLASLPFIRIINT 201
           P   IL++A  P+ +SL  +P +RI  T
Sbjct: 182 PASFILLLAVTPTVLSLLVMPLVRIYET 208

[Close]

BLAST of Lsi01G020770 vs. TAIR10
Match: AT2G34355.1 (AT2G34355.1 Major facilitator superfamily protein)

HSP 1 Score: 152.5 bits (384), Expect = 3.2e-37
Identity = 79/197 (40.10%), Postives = 125/197 (63.45%), Query Frame = 1


Query: 17  RWLMLFASLTIMAMNGSGYMFGLYSARIKSIFRYDQSALNFISFFKDLGGNLGVVSGLLY 76
           +W+   AS+ I + +G+ Y F +YS+ +KS   YDQS L+F+S FKD+GG  G++SG LY
Sbjct: 7   KWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFLY 66

Query: 77  EV--------APPWLVLSVGAVLNFFAYFMLWSAVSGRTAAPGLWLMCVYMGVAANSLSF 136
                       PW+V+ VG V  F  +F +W++V G  A P + LMC+++ +A +SL F
Sbjct: 67  TAMTSKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHSLPF 126

Query: 137 ANTAALVTCVRNFPLHRGC---LLKGYIGLSGAIMTQLYHAMY-GDDEPEGLILMIAWLP 196
            NTA +VT  RNF  + G    +++G++GLSGAI+ QLYHA+  G+  P   IL++A +P
Sbjct: 127 FNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLAIVP 186

Query: 197 SAISLASLPFIRIINTI 202
           + +   ++PF+R+  T+
Sbjct: 187 TLVMFLAMPFVRVYETV 203

[Close]

BLAST of Lsi01G020770 vs. NCBI nr
Match: gi|700199651|gb|KGN54809.1| (hypothetical protein Csa_4G507390 [Cucumis sativus])

HSP 1 Score: 352.8 bits (904), Expect = 4.5e-94
Identity = 184/221 (83.26%), Postives = 193/221 (87.33%), Query Frame = 1


Query: 2   AEAAAAGFLHKLLTGRWLMLFASLTIMAMNGSGYMFGLYSARIKSIFRYDQSALNFISFF 61
           A AA  GFLHKLLTGRWLMLFASL IMAMNGSGYMFGLYS  IKS+F YDQS LN ISFF
Sbjct: 5   ALAAVTGFLHKLLTGRWLMLFASLIIMAMNGSGYMFGLYSNHIKSVFGYDQSTLNLISFF 64

Query: 62  KDLGGNLGVVSGLLYEVAPPWLVLSVGAVLNFFAYFMLWSAVSGRTAAPGLWLMCVYMGV 121
           KDLG NLGVVSGLLYEVAPPWLVLSVGA+LNFF YFMLW AVSGRTAAPGL LMCVYM V
Sbjct: 65  KDLGANLGVVSGLLYEVAPPWLVLSVGAILNFFGYFMLWLAVSGRTAAPGLRLMCVYMSV 124

Query: 122 AANSLSFANTAALVTCVRNFPLHRGC---LLKGYIGLSGAIMTQLYHAMYGDDEPEGLIL 181
           AANSLSF NTAALVTC+RNFPLHRGC   LLKGYIGLSGAIMTQLYHAMYG+D PEGLIL
Sbjct: 125 AANSLSFGNTAALVTCLRNFPLHRGCLLGLLKGYIGLSGAIMTQLYHAMYGEDNPEGLIL 184

Query: 182 MIAWLPSAISLASLPFIRIINTINHQPPSQQP-QTILYPSL 219
           MIAWLPSAISLASLPFIR+IN+ N+Q    +P   +LY SL
Sbjct: 185 MIAWLPSAISLASLPFIRLINSNNNQRNDLKPFYNLLYISL 225

[Close]

BLAST of Lsi01G020770 vs. NCBI nr
Match: gi|778697768|ref|XP_004146405.2| (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus])

HSP 1 Score: 324.3 bits (830), Expect = 1.7e-85
Identity = 169/203 (83.25%), Postives = 178/203 (87.68%), Query Frame = 1


Query: 20  MLFASLTIMAMNGSGYMFGLYSARIKSIFRYDQSALNFISFFKDLGGNLGVVSGLLYEVA 79
           MLFASL IMAMNGSGYMFGLYS  IKS+F YDQS LN ISFFKDLG NLGVVSGLLYEVA
Sbjct: 1   MLFASLIIMAMNGSGYMFGLYSNHIKSVFGYDQSTLNLISFFKDLGANLGVVSGLLYEVA 60

Query: 80  PPWLVLSVGAVLNFFAYFMLWSAVSGRTAAPGLWLMCVYMGVAANSLSFANTAALVTCVR 139
           PPWLVLSVGA+LNFF YFMLW AVSGRTAAPGL LMCVYM VAANSLSF NTAALVTC+R
Sbjct: 61  PPWLVLSVGAILNFFGYFMLWLAVSGRTAAPGLRLMCVYMSVAANSLSFGNTAALVTCLR 120

Query: 140 NFPLHRGC---LLKGYIGLSGAIMTQLYHAMYGDDEPEGLILMIAWLPSAISLASLPFIR 199
           NFPLHRGC   LLKGYIGLSGAIMTQLYHAMYG+D PEGLILMIAWLPSAISLASLPFIR
Sbjct: 121 NFPLHRGCLLGLLKGYIGLSGAIMTQLYHAMYGEDNPEGLILMIAWLPSAISLASLPFIR 180

Query: 200 IINTINHQPPSQQP-QTILYPSL 219
           +IN+ N+Q    +P   +LY SL
Sbjct: 181 LINSNNNQRNDLKPFYNLLYISL 203

[Close]

BLAST of Lsi01G020770 vs. NCBI nr
Match: gi|1012359832|gb|KYP71016.1| (putative transporter MCH1 [Cajanus cajan])

HSP 1 Score: 261.5 bits (667), Expect = 1.4e-66
Identity = 129/216 (59.72%), Postives = 163/216 (75.46%), Query Frame = 1


Query: 8   GFLHKLLTGRWLMLFASLTIMAMNGSGYMFGLYSARIKSIFRYDQSALNFISFFKDLGGN 67
           GF + +LTGRW MLFASL IM++ G+ YMFG+YS  +K+   YDQS LN + FFKDLG N
Sbjct: 16  GFSYHVLTGRWFMLFASLLIMSVAGATYMFGIYSNEVKTSLGYDQSTLNLLGFFKDLGAN 75

Query: 68  LGVVSGLLYEVAPPWLVLSVGAVLNFFAYFMLWSAVSGRTAAPGLWLMCVYMGVAANSLS 127
           +GV+SGL+ E+ PPW+VLS+G ++NFF YFM+W AVSGR A P +W MC+Y+ + ANS +
Sbjct: 76  VGVISGLVNEITPPWVVLSIGVIMNFFGYFMIWLAVSGRIAKPQVWQMCLYICIGANSQT 135

Query: 128 FANTAALVTCVRNFPLHRGC---LLKGYIGLSGAIMTQLYHAMYGDDEPEGLILMIAWLP 187
           FANT ALVTCV+NFP  RG    LLKGY+GLSGAI+ QLYHA YGD  P+ LIL+IAWLP
Sbjct: 136 FANTGALVTCVKNFPGSRGSILGLLKGYVGLSGAIIAQLYHAFYGDHNPQALILLIAWLP 195

Query: 188 SAISLASLPFIRIINTINHQPPSQQPQTILYPSLHI 221
           +A+S   LP IRI+NTI HQP   +   + Y  L+I
Sbjct: 196 AAVSFLFLPTIRIMNTI-HQPKDNR---VFYHLLYI 227

[Close]

BLAST of Lsi01G020770 vs. NCBI nr
Match: gi|950954245|ref|XP_014496372.1| (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Vigna radiata var. radiata])

HSP 1 Score: 260.8 bits (665), Expect = 2.3e-66
Identity = 130/215 (60.47%), Postives = 163/215 (75.81%), Query Frame = 1


Query: 9   FLHKLLTGRWLMLFASLTIMAMNGSGYMFGLYSARIKSIFRYDQSALNFISFFKDLGGNL 68
           F + ++TGRW MLFASL IM++ G+ YMFG YS  +K+   YDQS LN +SFFKDLG N+
Sbjct: 12  FGYNVITGRWFMLFASLLIMSVAGATYMFGAYSNEVKTSLGYDQSTLNLLSFFKDLGANV 71

Query: 69  GVVSGLLYEVAPPWLVLSVGAVLNFFAYFMLWSAVSGRTAAPGLWLMCVYMGVAANSLSF 128
           GVVSGL+ E+ PPW+VLS+G ++NFF YFM+W AVSGR A P LW MC+Y+ + ANS +F
Sbjct: 72  GVVSGLVNEITPPWVVLSIGVLMNFFGYFMIWLAVSGRIAKPQLWKMCLYICIGANSQTF 131

Query: 129 ANTAALVTCVRNFPLHRGC---LLKGYIGLSGAIMTQLYHAMYGDDEPEGLILMIAWLPS 188
           ANT ALVTCV+NFP  RG    LLKGY+GLSGAI+TQLYHA YGD  P+ LIL+IAWLP+
Sbjct: 132 ANTGALVTCVKNFPGSRGSLLGLLKGYVGLSGAIITQLYHAFYGDHNPQALILLIAWLPA 191

Query: 189 AISLASLPFIRIINTINHQPPSQQPQTILYPSLHI 221
           A+S   LP IRI NT+ HQP + +   + Y  L+I
Sbjct: 192 AVSFLFLPTIRIFNTV-HQPKNNR---VFYHLLYI 222

[Close]

BLAST of Lsi01G020770 vs. NCBI nr
Match: gi|920682510|gb|KOM29282.1| (hypothetical protein LR48_Vigan641s007000 [Vigna angularis])

HSP 1 Score: 260.4 bits (664), Expect = 3.1e-66
Identity = 129/213 (60.56%), Postives = 162/213 (76.06%), Query Frame = 1


Query: 11  HKLLTGRWLMLFASLTIMAMNGSGYMFGLYSARIKSIFRYDQSALNFISFFKDLGGNLGV 70
           + ++TGRW MLFASL IM++ G+ YMFG YS  +K+   YDQS LN +SFFKDLG N+GV
Sbjct: 14  YNVITGRWFMLFASLLIMSVAGATYMFGAYSNEVKTSLGYDQSTLNLLSFFKDLGANVGV 73

Query: 71  VSGLLYEVAPPWLVLSVGAVLNFFAYFMLWSAVSGRTAAPGLWLMCVYMGVAANSLSFAN 130
           VSGL+ E+ PPW+VLS+G ++NFF YFM+W AVSGR A P LW MC+Y+ + ANS +FAN
Sbjct: 74  VSGLVNEITPPWVVLSIGVLMNFFGYFMIWLAVSGRIAKPQLWKMCLYICIGANSQTFAN 133

Query: 131 TAALVTCVRNFPLHRGC---LLKGYIGLSGAIMTQLYHAMYGDDEPEGLILMIAWLPSAI 190
           T ALVTCV+NFP  RG    LLKGY+GLSGAI+TQLYHA YGD  P+ LIL+IAWLP+A+
Sbjct: 134 TGALVTCVKNFPGSRGSLLGLLKGYVGLSGAIITQLYHAFYGDHNPQALILLIAWLPAAV 193

Query: 191 SLASLPFIRIINTINHQPPSQQPQTILYPSLHI 221
           S   LP IRI NT+ HQP + +   + Y  L+I
Sbjct: 194 SFLFLPTIRIFNTV-HQPKNNR---VFYHLLYI 222

The following BLAST results are available for this feature:

BLAST of Lsi01G020770 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Lsi01G020770 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L178_CUCSA	3.2e-94	83.26	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G507390 PE=4 SV=1	[more]
A0A151TVG0_CAJCA	9.6e-67	59.72	Putative transporter MCH1 OS=Cajanus cajan GN=KK1_010259 PE=4 SV=1	[more]
A0A0S3RYX4_PHAAN	2.1e-66	60.56	Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.04G333600 PE=...	[more]
A0A0L9TFQ4_PHAAN	2.1e-66	60.56	Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan641s007000 PE=4 SV=1	[more]
V7D0U4_PHAVU	1.4e-65	59.15	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_001G180500g PE=4 SV=1	[more]

BLAST of Lsi01G020770 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G39210.1	4.6e-60	56.19	Major facilitator superfamily protein	[more]
AT2G28120.1	5.2e-56	48.95	Major facilitator superfamily protein	[more]
AT1G18940.1	4.4e-39	40.29	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT1G74780.1	1.4e-37	41.35	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT2G34355.1	3.2e-37	40.10	Major facilitator superfamily protein	[more]

BLAST of Lsi01G020770 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700199651\|gb\|KGN54809.1\|	4.5e-94	83.26	hypothetical protein Csa_4G507390 [Cucumis sativus]	[more]
gi\|778697768\|ref\|XP_004146405.2\|	1.7e-85	83.25	PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus]	[more]
gi\|1012359832\|gb\|KYP71016.1\|	1.4e-66	59.72	putative transporter MCH1 [Cajanus cajan]	[more]
gi\|950954245\|ref\|XP_014496372.1\|	2.3e-66	60.47	PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Vigna radiata var. radiata]	[more]
gi\|920682510\|gb\|KOM29282.1\|	3.1e-66	60.56	hypothetical protein LR48_Vigan641s007000 [Vigna angularis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR010658	Nodulin-like

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi01G020770.1	Lsi01G020770.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 17..204 score: 2.2
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 1..206 score: 9.2E
None	No IPR available	PANTHER	PTHR21576:SF18	SUBFAMILY NOT NAMED	coord: 1..206 score: 9.2E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi01G020770	ClCG04G002340	Watermelon (Charleston Gray)	lsiwcgB139
Lsi01G020770	Cla97C04G069920	Watermelon (97103) v2	lsiwmbB137

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi01G020770	Wax gourd	lsiwgoB174
Lsi01G020770	Cucumber (Gy14) v1	cgylsiB428
Lsi01G020770	Cucurbita maxima (Rimu)	cmalsiB599
Lsi01G020770	Cucurbita moschata (Rifu)	cmolsiB585
Lsi01G020770	Cucurbita pepo (Zucchini)	cpelsiB050
Lsi01G020770	Wild cucumber (PI 183967)	cpilsiB249
Lsi01G020770	Cucumber (Chinese Long) v2	culsiB261
Lsi01G020770	Melon (DHL92) v3.5.1	lsimeB159
Lsi01G020770	Watermelon (97103) v1	lsiwmB151
Lsi01G020770	Cucumber (Gy14) v2	cgyblsiB238
Lsi01G020770	Melon (DHL92) v3.6.1	lsimedB167
Lsi01G020770	Cucumber (Chinese Long) v3	cuclsiB274