Lsi02G027540 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi02G027540
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationchr02 : 33755235 .. 33758488 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAAGCACCTACCTTTTTTCTTGCATTGACCATTTTTTAAAAAACTTGTTTAATTTCTCTTACATAAGCTAAGCCATGCGAAGAGGTTGTAATTAAGACACAATGGTTAGTGAAGCAAAAGGCAAAATCCCCAGTATAGATTTGTCAAAAATAGAGGGAGGTGGGAAAGAAGAATGGGGTTTAGCATGCAAAGAGATGAGAATTGGTTTAGAAGAATTTGGTTGTTTTTCAGTGGTTTATGACGAAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAACAGCTGTTTCATCTACCAATTGAAACAAGAATGAAGAACACAAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCTGTTCTTCCTCTTTATGAATCCATGGGTATTGACAATCCAATTGAGCTACAAGCAATTCACAATTTCGCCAATCTCATGTGGCCTGCTACAGAAAACAATCGCTTCTGGTAATTAATAATTAACACACACTACTTTTCTTTCTCAACTATTTACTTTCAATTTTTTTTTCTTAATTATTTGTTCATGTCTGCAAAAAAAAAAAAAAATTTATTGTTTTGGAAAACACTTTATTATTGGCACAAAAAATGTCATGTGTATTAAATAATAAAATTGTTTCAATTACAAATTTAATCTATAAATTTTAGGATTTGTGTCTATTTTTAGAGTCAAATGAATATTAGGGATATTATGTTTATTCTTCTTCACCCTTGTGACGGGGTTGCCCATTCTTAGCAAGGTGGGCTTTCTCTAATGGTGTGAATGGTCAATGGACCGAAGCACTGTCTTTGTAGGTGAGGCCTAGTGTTGAGCATGATGCTCGTGATTGTAATTCCTTTTATGTCAAATATATATATATATATATATATATCCTTGACTTTTTTAATTTTTAATTTTTGTTTCATATAGATCATTGACTTTTTTTAAAAAAAAAAAAATTTAAAATTAACCAATCTGTCATATATAGAATTTAATTTAATCAATTCAAAAATACTTTTGGCTCTAACATAGAATAATGAAAGTAACAATTTAATCCCTAAACTTTGATTTGTAATGATTTAGTCTCTTTATTTTCAATTTTATAGCAATTTAATATATGAACTTTAGTATGTATAATTTATTCACTATACTTTCAAATTTGTAGCAATTTAATCTCTAACTTAAAAAAAATCATAAAAAATAAATGACAATTTTATAATTTTAAGGACATAGACTTTTTAGCTCATAAATATATAGAAAATATTTAGGTGCATTCCAGACATCATCTTTCTTTGTGCATCTCACTAAATTATCCATTCACAATGTGCTACATGGTAAATTCTTTTTACTTTTGTTTTGAAATATTTTAATATTTTTTATATATGTAGTGAAAATGGAGTGCAGTGCATCTTGAGATGCACCTGAGTATTACTCTAAATATATTGGGGTCAGACTTTTAATTAGTTTGTCGAACCACCCAATTAAAGAAATCTTATTAAATATTAACACTAATTATTTCTCTCTTTTTATGTTCTTCCCTCCAACTGTTGTAGTACGTTAACGTAGTTCAATGGGTGAAGATATGGCTTTAAGATTCATAAAAGTTGAAAAAAATGGGGTGTTATTGGTTTTGATTATCTTCTTTTAATTACATGAAACTGAATAATGATTGGCAGTGAAACTGTTGAACGTTTCTCAACGTTGGTGGCGAAATTGGAGAAGATGGTAACAAAAATGGTAATGGAAAGCTATGGAGTATCGAAAGTTGTGAGTGAGTCAAACATGGAGTCCACCGATTACCTTTTTCGATGCTACAAATACAGAGTGCCTGAGAAAGATGAAAGCGATGTGGGATTGCATTCCCATACAGATCTTACTTTCTTTTCCATAATTCATCAGCATCTCATTTCTGGCCTTCAAATTCAATCACTTGATGATCAATGGATTGATATTCACCCCTCAACCCATTGCTCCTTTGTTGTCATGGCTGGAGATGCACTCATGGTAATTAACCCCACAATCCCTTTTTTCTTTTTCATAACTAAATATTTACTCTCCCAACTTTTATCACCTAATCGAGATGGTAAAATAAATAAATTACAAATTTACTTCATCAACTTTAAGACATTTCTCGCTATTTTGAAGTTTGAAGTTTATTTCAAAACAATTATTGTAAGCTTTATTTTACCATTAAGTTCATAGACAAATCATACAGATAAGGCAAATAAAGACACATGACACATTCGTGAAATATATATTTGTTGTCGTCTTCCCTCCTTTCAACCTCACATGGGTGTTTATGATATTTTTTTTTTTTTTTTTTTAGCCCTAACTCCCAAATTTACTTCTCATGTGTTTTTAGATTTGTTTACTTAAAACAACACATAAGCAAACACAAACTTTTTCATCCCATCCATGAATTTAACAAGAAAAACCTTCCACGGTTATGTTTTAAGGGAAAAATAACTTCAAACTTCAAGGAAGAGTAATAATTGAAAACCAACCACATACTTCAAGGGTACTTTTGATAATTCAACATTTTTAAAATTCACGAATCTAATAGATACAAGATTAATAAAAATCTACTTGTAAATGTAAACTTCATAATATAAAGACTAAACTTATAATTTAAGCGTATGTTATGTTATGGATTGGTGTAGGCATGGAGCAACGAGAGAATAAGGTCATGTAGGCACCAAGTGATAATGAAAGGAGAGGAAACAAGATACTCAATTGGGCTGTTTTCATTCAAGAGTGAGACGATTGCTGTGCCTGAAGAATTTGTGGATGATGCCAACCCATTACGATACAAGCCTTTTAATCACTATGATTTCCTTACCTATGACAAAGCCAAGGCTTCTCATCAAAATGTCTCTCGTATCAAAGATTATTGTGGCATTTGATATCAACAAAGCCTTCCTCATATGCATCCATCATCAACAACCCTATATTTCACCTAATTTCCATACTCGTCGCGTTTGCTCTTAACCGCTAAATTTTTTTAGTGGAATTCACTTCTCTATTAAACACAAAATAAAGGTTATGTTTTCCTTTCTTTCTTTTATAAATTCAGACATAAATGGAATACTACTCCCCATATAATTTGATGTTGATTAAAAAATGTGCATATGTCCTTATAAGAATTATAGATAAGTCTTTTTGCTGTTTTTCACTCCTCACATTGTGGTGATTCTTGGAATAAAAGTTGTAGCAAATATATAGTATTGTTTTTCTAAATTA

mRNA sequence

AAAAAAAAAAAAGCACCTACCTTTTTTCTTGCATTGACCATTTTTTAAAAAACTTGTTTAATTTCTCTTACATAAGCTAAGCCATGCGAAGAGGTTGTAATTAAGACACAATGGTTAGTGAAGCAAAAGGCAAAATCCCCAGTATAGATTTGTCAAAAATAGAGGGAGGTGGGAAAGAAGAATGGGGTTTAGCATGCAAAGAGATGAGAATTGGTTTAGAAGAATTTGGTTGTTTTTCAGTGGTTTATGACGAAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAACAGCTGTTTCATCTACCAATTGAAACAAGAATGAAGAACACAAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCTGTTCTTCCTCTTTATGAATCCATGGGTATTGACAATCCAATTGAGCTACAAGCAATTCACAATTTCGCCAATCTCATGTGGCCTGCTACAGAAAACAATCGCTTCTGTGAAACTGTTGAACGTTTCTCAACGTTGGTGGCGAAATTGGAGAAGATGGTAACAAAAATGGTAATGGAAAGCTATGGAGTATCGAAAGTTGTGAGTGAGTCAAACATGGAGTCCACCGATTACCTTTTTCGATGCTACAAATACAGAGTGCCTGAGAAAGATGAAAGCGATGTGGGATTGCATTCCCATACAGATCTTACTTTCTTTTCCATAATTCATCAGCATCTCATTTCTGGCCTTCAAATTCAATCACTTGATGATCAATGGATTGATATTCACCCCTCAACCCATTGCTCCTTTGTTGTCATGGCTGGAGATGCACTCATGGCATGGAGCAACGAGAGAATAAGGTCATGTAGGCACCAAGTGATAATGAAAGGAGAGGAAACAAGATACTCAATTGGGCTGTTTTCATTCAAGAGTGAGACGATTGCTGTGCCTGAAGAATTTGTGGATGATGCCAACCCATTACGATACAAGCCTTTTAATCACTATGATTTCCTTACCTATGACAAAGCCAAGGCTTCTCATCAAAATGTCTCTCGTATCAAAGATTATTGTGGCATTTGATATCAACAAAGCCTTCCTCATATGCATCCATCATCAACAACCCTATATTTCACCTAATTTCCATACTCGTCGCGTTTGCTCTTAACCGCTAAATTTTTTTAGTGGAATTCACTTCTCTATTAAACACAAAATAAAGGTTATGTTTTCCTTTCTTTCTTTTATAAATTCAGACATAAATGGAATACTACTCCCCATATAATTTGATGTTGATTAAAAAATGTGCATATGTCCTTATAAGAATTATAGATAAGTCTTTTTGCTGTTTTTCACTCCTCACATTGTGGTGATTCTTGGAATAAAAGTTGTAGCAAATATATAGTATTGTTTTTCTAAATTA

Coding sequence (CDS)

ATGGTTAGTGAAGCAAAAGGCAAAATCCCCAGTATAGATTTGTCAAAAATAGAGGGAGGTGGGAAAGAAGAATGGGGTTTAGCATGCAAAGAGATGAGAATTGGTTTAGAAGAATTTGGTTGTTTTTCAGTGGTTTATGACGAAGTTTCTTTGGAGCTTCATAACTCCATTTTTGAAGCAACAAAACAGCTGTTTCATCTACCAATTGAAACAAGAATGAAGAACACAAGTGAAAAGCCTTACCATGGCTATTTTGGTGGATACTCTGTTCTTCCTCTTTATGAATCCATGGGTATTGACAATCCAATTGAGCTACAAGCAATTCACAATTTCGCCAATCTCATGTGGCCTGCTACAGAAAACAATCGCTTCTGTGAAACTGTTGAACGTTTCTCAACGTTGGTGGCGAAATTGGAGAAGATGGTAACAAAAATGGTAATGGAAAGCTATGGAGTATCGAAAGTTGTGAGTGAGTCAAACATGGAGTCCACCGATTACCTTTTTCGATGCTACAAATACAGAGTGCCTGAGAAAGATGAAAGCGATGTGGGATTGCATTCCCATACAGATCTTACTTTCTTTTCCATAATTCATCAGCATCTCATTTCTGGCCTTCAAATTCAATCACTTGATGATCAATGGATTGATATTCACCCCTCAACCCATTGCTCCTTTGTTGTCATGGCTGGAGATGCACTCATGGCATGGAGCAACGAGAGAATAAGGTCATGTAGGCACCAAGTGATAATGAAAGGAGAGGAAACAAGATACTCAATTGGGCTGTTTTCATTCAAGAGTGAGACGATTGCTGTGCCTGAAGAATTTGTGGATGATGCCAACCCATTACGATACAAGCCTTTTAATCACTATGATTTCCTTACCTATGACAAAGCCAAGGCTTCTCATCAAAATGTCTCTCGTATCAAAGATTATTGTGGCATTTGA

Protein sequence

MVSEAKGKIPSIDLSKIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATENNRFCETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVGLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSCRHQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQNVSRIKDYCGI
Homology
[Close]
BLAST of Lsi02G027540 vs. Swiss-Prot
Match: AOP1L_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 8.3e-59
Identity = 120/301 (39.87%), Postives = 181/301 (60.13%), Query Frame = 1 

Query: 8   KIPSIDLSKIE-GGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFH 67
           ++P ID S      G  +W     ++   LE++GCF   +D++S+EL+ S+FEA + LF 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLFE 72

Query: 68  LPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATENNRFCE 127
           LPI T+ +N S KP+HGY        LYES+GID+   L+ +++F   +WP   N    E
Sbjct: 73  LPIPTKQRNVSSKPFHGYL----CHNLYESLGIDDANVLEKVNDFTQQLWPDHGNKSISE 132

Query: 128 TVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESD---- 187
           T+  FS  + +L+ MV +M+MES+G+   + E ++ ST YL R  KY  P  D+ D    
Sbjct: 133 TIHLFSEQLVELDLMVRRMIMESFGIENYIDE-HLNSTYYLTRLMKYTSPPDDDDDDEET 192

Query: 188 -VGLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERI 247
            +GL SHTD    +I+HQ+ + GL++++ DD+WI + PS   S +VM GD+L A  N R+
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQD-SVLVMVGDSLCALLNGRL 252

Query: 248 RSCRHQVIMKGEETRYSIGLFSFKSETIAV--PEEFVDDANPLRYKPFNHYDFLTYDKAK 301
            S  H+VIM G++TRYS GLFS     + +  PEE VD  +P  +KPF + DFL + + +
Sbjct: 253 HSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTE 307

[Close]
BLAST of Lsi02G027540 vs. Swiss-Prot
Match: AOP1C_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana GN=AOP1 PE=2 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.1e-58
Identity = 119/302 (39.40%), Postives = 181/302 (59.93%), Query Frame = 1 

Query: 8   KIPSIDLSKIE-GGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFH 67
           ++P ID S      G  +W     ++   LE++GCF   +D++S+EL+ S+FEA + LF 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 72

Query: 68  LPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATENNRFCE 127
           LPI T+ +N S KP+HGY        LYES+GI++   L+ +++F   +WP   N    E
Sbjct: 73  LPIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 132

Query: 128 TVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESD---- 187
           T+  FS  + +L+ MV +M+MES+G+   + E ++ ST YL R  KY  P  D+ D    
Sbjct: 133 TIHLFSEQLVELDLMVRRMIMESFGIENYIDE-HLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 188 --VGLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNER 247
             +GL SHTD    +I+HQ+ + GL++++ DD+WI + PS   S +VM GD+L A  N R
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQD-SVLVMVGDSLCALLNGR 252

Query: 248 IRSCRHQVIMKGEETRYSIGLFSFKSETIAV--PEEFVDDANPLRYKPFNHYDFLTYDKA 301
           + S  H+VIM G++TRYS GLFS     + +  PEE VD  +P  +KPF + DFL + + 
Sbjct: 253 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQT 308

[Close]
BLAST of Lsi02G027540 vs. Swiss-Prot
Match: AOP1V_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana GN=AOP1 PE=2 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 7.0e-58
Identity = 119/300 (39.67%), Postives = 181/300 (60.33%), Query Frame = 1 

Query: 8   KIPSIDLSKIE-GGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFH 67
           ++P ID S      G  +W     ++   LE++GCF   +D++S+EL+ S+FEA + LF 
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 65

Query: 68  LPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATENNRFCE 127
           LPI T+ +N S K +HGY        LYES+GI++   L+ +++F   +WP   N    E
Sbjct: 66  LPIPTKQRNVSSKLFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 125

Query: 128 TVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESD---- 187
           T+  FS  + +L+ MV +M+MES+G+ K + E ++ ST YL R  KY  P  D+ D    
Sbjct: 126 TIHLFSEQLVELDLMVRRMIMESFGIEKYIDE-HLNSTYYLTRLMKYTSPPDDDDDEETK 185

Query: 188 VGLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERIR 247
           +GL SHTD    +I+HQ+ + GL++++ DD+WI + PS   S +VM GD+L A  N R+ 
Sbjct: 186 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQD-SVLVMVGDSLCALLNGRLH 245

Query: 248 SCRHQVIMKGEETRYSIGLFSFKSETIAV--PEEFVDDANPLRYKPFNHYDFLTYDKAKA 301
           S  H+VIM G++TRYS GLFS     + +  PEE VD  +P  +KPF + DFL + + +A
Sbjct: 246 SPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEA 299

[Close]
BLAST of Lsi02G027540 vs. Swiss-Prot
Match: AOP2V_ARATH (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.7e-27
Identity = 65/186 (34.95%), Postives = 110/186 (59.14%), Query Frame = 1 

Query: 8   KIPSIDLS-KIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFH 67
           ++P I+L+ K    G  +W     ++R  LE+FGCF   YD+VSLEL  SI +  ++LF 
Sbjct: 7   QLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELFA 66

Query: 68  LPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATE-NNRFC 127
           LP+ET+ +N   KPY GY    +   L ES+GI N   L+ ++ F   +WP  + N    
Sbjct: 67  LPVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENVNEFTQQLWPDGDGNENIS 126

Query: 128 ETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVGL 187
           +T++ F+  + +++ MV +MVMES+G+ K + + +++ST+Y  R  KY  P + +++  +
Sbjct: 127 KTIQLFAEKLVEIDVMVRRMVMESFGIEKYI-DDHLKSTEYRMRLMKYIAPPEGDANTTV 186

Query: 188 HSHTDL 192
             + DL
Sbjct: 187 DDYADL 188

[Close]
BLAST of Lsi02G027540 vs. Swiss-Prot
Match: AOP2L_ARATH (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 2.9e-27
Identity = 65/186 (34.95%), Postives = 110/186 (59.14%), Query Frame = 1 

Query: 8   KIPSIDLS-KIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFH 67
           ++P I+L+ K    G  +W     ++R  L++FGCF   YD+VSLEL  SI +  ++LF 
Sbjct: 7   QLPLINLADKTLEPGSSKWAEVRSDVRKALQDFGCFEASYDKVSLELQESIMKTMEELFA 66

Query: 68  LPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATE-NNRFC 127
           LP+ET+ +N   KPY GY    +   L ES+GI N   L+ I+ F   +WP  + N    
Sbjct: 67  LPVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENINEFTQQLWPHGDGNENIS 126

Query: 128 ETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVGL 187
           +T++ F+  + +++ MV +MVMES+G+ K + + +++ST+Y  R  KY  P + +++  +
Sbjct: 127 KTIQLFAEKLVEIDVMVRRMVMESFGIEKYI-DDHLKSTEYRMRLMKYIAPPEGDANTTV 186

Query: 188 HSHTDL 192
             + DL
Sbjct: 187 DDYADL 188

[Close]
BLAST of Lsi02G027540 vs. TrEMBL
Match: A0A0A0K8Y2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G434960 PE=4 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 4.8e-106
Identity = 194/242 (80.17%), Postives = 212/242 (87.60%), Query Frame = 1 

Query: 1   MVSEAKGK--IPSIDLSKIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIF 60
           MV EAK K  IP IDLSK+E G KE WGLAC+EMR GLE+FGCFSVVYD+VSLELHNSIF
Sbjct: 1   MVCEAKSKVVIPIIDLSKLEEGTKE-WGLACREMRFGLEQFGCFSVVYDKVSLELHNSIF 60

Query: 61  EATKQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPA 120
           EATK+LFHLPIETRMKNTSEKPYHGYFGGYS LPLYESM IDNPI+LQ   +FANLMWP 
Sbjct: 61  EATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPT 120

Query: 121 TENNRFCETVERFSTLVAKLEKMVTKMVMESYGVS-KVVSESNMESTDYLFRCYKYRVPE 180
           TENN FC  VERFSTLVAKLE MVTKMV+ESYGVS KVVSE  MEST+YL R +KYRV E
Sbjct: 121 TENNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAE 180

Query: 181 KDESDVGLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWS 240
           KDE+DVGLHSHTDLTF SIIHQHLISGLQIQ LDDQW+++HPS+HCSF VMAGDALM  +
Sbjct: 181 KDENDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMVTT 240

[Close]
BLAST of Lsi02G027540 vs. TrEMBL
Match: M5WSX6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008973mg PE=3 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 3.6e-93
Identity = 168/315 (53.33%), Postives = 223/315 (70.79%), Query Frame = 1 

Query: 1   MVSEAKGKIPSIDLSKIE-GGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFE 60
           M SE + K+P IDLS      G E W LA K ++  LEE+GCF  V+ +V L+LHNSIF 
Sbjct: 1   MGSETQPKLPVIDLSNENLKPGTEAWVLASKHVKYALEEYGCFEAVFHKVPLQLHNSIFS 60

Query: 61  ATKQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPAT 120
             + LF LPIET+ + TSE+PYH YFG YS LPLYES+G+DNP  ++    F  +MWPA 
Sbjct: 61  VVEDLFDLPIETKRQQTSERPYHSYFGQYSFLPLYESLGVDNPTTVEGAKGFTKIMWPA- 120

Query: 121 ENNRFCETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKD 180
            N+ FCE+ + FS LVA+L++MVT+MV + YGV ++  +S+M ST YL RC+KYR  +++
Sbjct: 121 GNDHFCESAQSFSKLVAELDQMVTRMVFDIYGVERLY-DSHMASTTYLLRCFKYREQKEN 180

Query: 181 ESDVGLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNE 240
           E++VGL  H D TF SI+HQ  + GLQ++S D QWI+  PS   SF+VMAGDA MAWSN+
Sbjct: 181 ETNVGLRPHKDKTFTSILHQSQVHGLQVRSKDGQWIEAEPSP-SSFLVMAGDAFMAWSND 240

Query: 241 RIRSCRHQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAK 300
           R+RSC HQVIMK  +TRYS+GLFSF S  + VPEE  DD +PL YKPF+H+ FL +D+  
Sbjct: 241 RVRSCDHQVIMKENKTRYSVGLFSFNSGILQVPEELTDDQHPLLYKPFDHFSFLRFDQTP 300

Query: 301 ASHQNVSRIKDYCGI 315
            + ++   IK YCG+
Sbjct: 301 EAKKSECPIKAYCGV 312

[Close]
BLAST of Lsi02G027540 vs. TrEMBL
Match: W9SCZ0_9ROSA (Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis GN=L484_019468 PE=3 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 4.2e-86
Identity = 155/296 (52.36%), Postives = 211/296 (71.28%), Query Frame = 1 

Query: 5   AKGKIPSIDLSKIE-GGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQ 64
           A+ KIP ID +K     G +EW LACKE+R G EE+GCF  VYD++ ++LHNS+F A + 
Sbjct: 6   AEHKIPVIDFTKKNLKPGTDEWVLACKEVRNGFEEYGCFEAVYDKIPVDLHNSVFAAAES 65

Query: 65  LFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATENNR 124
           LF LP+ET+M+ TSE+PYH Y+G +SV+PLYES+G+DNP  LQ    F NLMWPA  N  
Sbjct: 66  LFDLPLETKMQKTSERPYHSYYGQFSVVPLYESLGVDNPTTLQGSRKFTNLMWPA-GNEN 125

Query: 125 FCETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDV 184
           F ET +  S L+ +L ++ T+MV +SYGV + +  SNM  T YL R +KYR  + +E+DV
Sbjct: 126 FRETTQSMSKLLVELYELATRMVFDSYGVQR-LHGSNMALTSYLLRFFKYRTRQPNETDV 185

Query: 185 GLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERIRS 244
           GL  H D TF SI++QH I GLQ++S D QWID+ PS   SF+++AGD + AWSN+R+R 
Sbjct: 186 GLQCHKDRTFMSIVNQHQIEGLQVKSKDGQWIDVKPSA-SSFLIIAGDIVGAWSNDRVRP 245

Query: 245 CRHQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAK 300
           C HQVIMK  +TRYSIGLF+F +  I VPE+ VD+  PL+YKP +H+ +L + + +
Sbjct: 246 CEHQVIMKENKTRYSIGLFAFINGVIDVPEDLVDEKYPLKYKPIDHFGWLRFQEQR 298

[Close]
BLAST of Lsi02G027540 vs. TrEMBL
Match: V4ST91_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027420mg PE=3 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 4.7e-85
Identity = 161/317 (50.79%), Postives = 218/317 (68.77%), Query Frame = 1 

Query: 1   MVSEAKGKIPSIDLSKIE-GGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFE 60
           M S+ K KIP IDLSK +   G   W   CK++R   EEFGCF  +Y ++S E  + +  
Sbjct: 1   MGSQTKSKIPVIDLSKQDLKSGTSTWLSTCKDVRHAFEEFGCFEAIYQKISQEFRSEVLA 60

Query: 61  ATKQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPAT 120
           +T++LF +PIE ++KNTS KPY  Y+G Y+++PLYES+ ID P    A  +F NLMWPA 
Sbjct: 61  STEELFDVPIEIKVKNTSTKPYFEYYGQYTIIPLYESLAIDYPDTRNATQSFTNLMWPA- 120

Query: 121 ENNRFCETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKD 180
            N+ FCE+    S LV +L+KMVT+M+ ESYGV     +S + S +YL R +KYR PE +
Sbjct: 121 GNDCFCESSLSMSELVVELDKMVTRMLFESYGVEGYY-DSYIGSVNYLLRYFKYRAPEPN 180

Query: 181 ESDVGLHSHTDLTFFSIIHQ-HLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSN 240
           E+ +GL  HTD T  SIIHQ + I+GLQ+Q+ D +WID+ PS   SF+VMAGDALMAW N
Sbjct: 181 ETKMGLTPHTDKTMTSIIHQINHINGLQVQAKDGEWIDVEPSP-SSFIVMAGDALMAWGN 240

Query: 241 ERIRSCRHQVIM-KGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDK 300
           +RIR CRHQVIM    ETRYS+GLFSF S  + +P+E  D+  PL+YKPF+H+ FL +++
Sbjct: 241 DRIRPCRHQVIMDNASETRYSLGLFSFSSGVVDIPKELGDETQPLKYKPFDHFGFLHFNQ 300

Query: 301 AKASHQNVSRIKDYCGI 315
           ++   ++ S IK YCGI
Sbjct: 301 SEEGKKSASSIKAYCGI 314

[Close]
BLAST of Lsi02G027540 vs. TrEMBL
Match: V7APY1_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_010G152400g PE=3 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 1.8e-84
Identity = 144/313 (46.01%), Postives = 224/313 (71.57%), Query Frame = 1 

Query: 3   SEAKGKIPSIDLSKIE-GGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEAT 62
           SEA  ++P +D +  E   G  +WG AC+ +R  LE+ GCF  +YDEV +EL+NS+F   
Sbjct: 4   SEAVCEVPVVDFTDKELKPGTRKWGSACEVIRNALEDHGCFYALYDEVPMELYNSVFTLM 63

Query: 63  KQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATEN 122
           ++ F LP+ET+M+  S+KPYHGY+G ++ +PLYES+GI++P+ ++ I NF  LMWPA  +
Sbjct: 64  EEQFDLPLETKMQKISDKPYHGYYGQHAHVPLYESLGINHPLTMEGIQNFTKLMWPAGYD 123

Query: 123 NRFCETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDES 182
           + FCE++  ++ L+ +L+ M  +MV + YGV +   +S +EST+Y+ R +KYR+P+KDE+
Sbjct: 124 H-FCESMSMYAKLLVELDHMTKRMVFDGYGVEQRHCDSLLESTNYMLRSFKYRLPQKDEN 183

Query: 183 DVGLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERI 242
           D+GLH+HTD +FF+I+HQ+ ++GLQ++  +  WIDI PS    F+++AGDA   WSN+RI
Sbjct: 184 DLGLHAHTDTSFFTILHQNNVNGLQVKFKNGDWIDIDPSPF-MFLILAGDAFKVWSNDRI 243

Query: 243 RSCRHQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKAS 302
             C H+VI+ G++ RYS+GLFS   + +   EE VD+ +P  YKPF+HY++L++   K +
Sbjct: 244 HCCEHRVIISGKKERYSMGLFSLGGKMVETQEELVDEEHPRCYKPFDHYEYLSFCATKKA 303

Query: 303 HQNVSRIKDYCGI 315
            Q+ SRIK +CGI
Sbjct: 304 LQSHSRIKAFCGI 314

[Close]
BLAST of Lsi02G027540 vs. TAIR10
Match: AT1G52800.1 (AT1G52800.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 279.6 bits (714), Expect = 2.3e-75
Identity = 137/309 (44.34%), Postives = 197/309 (63.75%), Query Frame = 1 

Query: 8   KIPSIDLSKIEG--GGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLF 67
           K+P +DL+  +        W    +E    LEE+GCF  VYD V+ +L +SIF A ++LF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 68  HLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATENNRFC 127
            LP ET+ KN +EKPYHGY G   V+PL+E +G+D     +    F +LMWP   N+RFC
Sbjct: 69  DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWPQG-NDRFC 128

Query: 128 ETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVGL 187
            TV  FS  VA+L+++V +M+ E+YGV K   ES++ S  YL +  KY  P +  S    
Sbjct: 129 NTVHTFSNAVAELDRLVVRMIFENYGVEKHY-ESHVGSKTYLLKFLKYLAPPESISMPAF 188

Query: 188 HSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSCR 247
             HTD TF SI+HQ+ ++GL+++S D +WI +      S+VVMAGD  M WSN+RIRSC 
Sbjct: 189 PQHTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPK-SYVVMAGDISMGWSNDRIRSCE 248

Query: 248 HQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQNV 307
           H+V M+G++TRY++GLFSF ++ +++PEE VDD +PL YKPF++   + +   K   +  
Sbjct: 249 HRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREAN 308

Query: 308 SRIKDYCGI 315
           S +K YCGI
Sbjct: 309 STLKAYCGI 314

[Close]
BLAST of Lsi02G027540 vs. TAIR10
Match: AT1G52790.1 (AT1G52790.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 257.7 bits (657), Expect = 9.4e-69
Identity = 128/308 (41.56%), Postives = 198/308 (64.29%), Query Frame = 1 

Query: 8   KIPSIDLSKIE-GGGKEEWGLACKEMRIGLEEFGCFSV-VYDEVSLELHNSIFEATKQLF 67
           KIP++D S+ +   G + W    + +R  LEE+GCF + + D+  L+L + +F +   LF
Sbjct: 4   KIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDLF 63

Query: 68  HLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATENNRFC 127
            LP +T+MKN  +KP +GY G    LPL+ES+GIDN   L+A  +F  LMWP   N  F 
Sbjct: 64  DLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWPQG-NEHFS 123

Query: 128 ETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVGL 187
           E + +++   A+L++MVT+MV +SY V K   +  +EST YL R  K R P  +   +G 
Sbjct: 124 ECLYKYAEFAAELDQMVTRMVFQSYNVEKYY-DPYIESTTYLLRVLKNRAPNNENPTLGF 183

Query: 188 HSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSCR 247
            +HTD +F +I+HQ  ++GL++++ + + I+I+ S+   F+V+AGDALMAWSN+R+ S R
Sbjct: 184 VTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVWSPR 243

Query: 248 HQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQNV 307
           HQV++ GE  RYS+G+F+F + T+ VPEE +D  +PL YKPF+H   L + +    +++ 
Sbjct: 244 HQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYKSE 303

Query: 308 SRIKDYCG 314
             IK YCG
Sbjct: 304 CPIKAYCG 309

[Close]
BLAST of Lsi02G027540 vs. TAIR10
Match: AT1G52820.1 (AT1G52820.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 255.0 bits (650), Expect = 6.1e-68
Identity = 124/310 (40.00%), Postives = 199/310 (64.19%), Query Frame = 1 

Query: 8   KIPSIDLS-KIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFH 67
           ++P ID S K    G+ EW L   +++  L+++G F   +D +  EL  S+F A ++LF 
Sbjct: 11  RLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFD 70

Query: 68  LPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATENNRFCE 127
           LP++T+++N S+KP+HGY G Y ++PLYESMGID+    + +  F   +WP   N  F  
Sbjct: 71  LPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWPQG-NISFST 130

Query: 128 TVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDVGLH 187
           T++ FS  +++L+  + +M+MES+G+ K + E ++ ST+YL R  KY+ P+ +E+ VGL+
Sbjct: 131 TIQSFSKKLSELDITIRRMIMESFGLDKYIDE-HLHSTNYLLRVMKYKGPDTEETKVGLN 190

Query: 188 SHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERIRSCRH 247
           +HTD    +I++Q+ + GL++Q+ D  WI + P T  SF VM GD+L A  N R+ S  H
Sbjct: 191 AHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKP-TQDSFTVMIGDSLYALLNGRLHSPYH 250

Query: 248 QVIMKGEETRYSIGLFSF--KSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASHQN 307
           +V+M G ETRYS+GLFS       ++ P+E VD+ +P  +KPF+H +FL +   +A  ++
Sbjct: 251 RVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAGQRS 310

Query: 308 VSRIKDYCGI 315
            S +K YCGI
Sbjct: 311 QSALKTYCGI 317

[Close]
BLAST of Lsi02G027540 vs. TAIR10
Match: AT1G15540.1 (AT1G15540.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 229.6 bits (584), Expect = 2.7e-60
Identity = 131/314 (41.72%), Postives = 181/314 (57.64%), Query Frame = 1 

Query: 8   KIPSIDLS-KIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFH 67
           ++P I+LS K      E W      +R  +E  G F   Y+     LH SI EA K+L  
Sbjct: 8   ELPIINLSDKNLKQNTELWSFTRDSVREAMEHHGWFVAEYNNFPTGLHQSILEAAKELLD 67

Query: 68  LPIETRMKNTSEKPYHGYFGGYSV-LPLYESMGIDNPIELQAIHNFANLMWPAT--ENNR 127
           LP+E ++KN + K  HGY    S   P++E +GID   ++Q    F+ LMWP    +N+R
Sbjct: 68  LPVEIKLKNENHKAGHGYITMMSDGQPVHEGLGIDQVNDVQQCRGFSRLMWPDDHDDNDR 127

Query: 128 FCETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESDV 187
           FCETV  ++ + A+LE++V +M+ ESY V K  +E  +  T YL R  KYR     E + 
Sbjct: 128 FCETVHAYAKMQAELEQLVIRMLFESYNVEKY-TEKYIGGTRYLLRLLKYRRLPNGEPNR 187

Query: 188 GLHSHTDLTFFSIIHQHLISGLQIQS-LDDQWIDIHPSTHCSFVVMAGDALMAWSNERIR 247
              SHTD +F SI+HQ+ I+GL ++S  +D W    PS    FVV+AGDA+MAWSN+RI+
Sbjct: 188 KFISHTDKSFISILHQNHITGLMLKSEKEDVWYHFTPSP-TRFVVIAGDAIMAWSNDRIK 247

Query: 248 SCRHQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKASH 307
           +C H+V M+  E RYS+G FSF+   I+ PEE VD  +PL Y PF+H   L Y +    H
Sbjct: 248 ACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLLEYYETLEVH 307

Query: 308 QNVSR--IKDYCGI 315
               R   K YCGI
Sbjct: 308 LKAHRTMTKAYCGI 319

[Close]
BLAST of Lsi02G027540 vs. TAIR10
Match: AT4G03070.1 (AT4G03070.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 226.9 bits (577), Expect = 1.8e-59
Identity = 119/302 (39.40%), Postives = 181/302 (59.93%), Query Frame = 1 

Query: 8   KIPSIDLSKIE-GGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEATKQLFH 67
           ++P ID S      G  +W     ++   LE++GCF   +D++S+EL+ S+FEA + LF 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 72

Query: 68  LPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATENNRFCE 127
           LPI T+ +N S KP+HGY        LYES+GI++   L+ +++F   +WP   N    E
Sbjct: 73  LPIPTKQRNVSSKPFHGYL----CHNLYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 132

Query: 128 TVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDESD---- 187
           T+  FS  + +L+ MV +M+MES+G+   + E ++ ST YL R  KY  P  D+ D    
Sbjct: 133 TIHLFSEQLVELDLMVRRMIMESFGIENYIDE-HLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 188 --VGLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNER 247
             +GL SHTD    +I+HQ+ + GL++++ DD+WI + PS   S +VM GD+L A  N R
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQD-SVLVMVGDSLCALLNGR 252

Query: 248 IRSCRHQVIMKGEETRYSIGLFSFKSETIAV--PEEFVDDANPLRYKPFNHYDFLTYDKA 301
           + S  H+VIM G++TRYS GLFS     + +  PEE VD  +P  +KPF + DFL + + 
Sbjct: 253 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQT 308

[Close]
BLAST of Lsi02G027540 vs. NCBI nr
Match: gi|659122281|ref|XP_008461057.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo])

HSP 1 Score: 545.8 bits (1405), Expect = 4.9e-152
Identity = 263/317 (82.97%), Postives = 288/317 (90.85%), Query Frame = 1 

Query: 1   MVSEAKGK--IPSIDLSKIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIF 60
           MV EAK K  IP+IDLSK+E G K EWGLACKEMR GLEEFGCFSVVYD+VSLELHNSI 
Sbjct: 1   MVCEAKSKVVIPTIDLSKLEVGTK-EWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIV 60

Query: 61  EATKQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPA 120
           EATK+LFHLPIETRMKNTSEKPYHGYFGGYS LPLYESM IDNPI+L++  +FANLMWP 
Sbjct: 61  EATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPT 120

Query: 121 TENNRFCETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEK 180
            +NN FC TVERFSTLVAKLEKMVTKMV+ESYGVSKVV+E  MEST+YL R +KYRV EK
Sbjct: 121 RDNNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEK 180

Query: 181 DESDVGLHSHTDLTFFSIIHQHLISGLQIQSL-DDQWIDIHPSTHCSFVVMAGDALMAWS 240
           DE+DVGLHSHTDLTFFSIIHQHLISGLQIQSL DDQW+D+HPS+HCSF+VMAGDALMAWS
Sbjct: 181 DETDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWS 240

Query: 241 NERIRSCRHQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDK 300
           NERIRSCRH+VIM+GEETRYSIG+FSFK+ TI VP+ FVDDANPLRYKPFNHYDFLTYDK
Sbjct: 241 NERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDK 300

Query: 301 AKASHQNVSRIKDYCGI 315
           A ASH  +SRIKDYCG+
Sbjct: 301 ANASHNTISRIKDYCGL 316

[Close]
BLAST of Lsi02G027540 vs. NCBI nr
Match: gi|449436585|ref|XP_004136073.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus])

HSP 1 Score: 538.1 bits (1385), Expect = 1.0e-149
Identity = 260/317 (82.02%), Postives = 284/317 (89.59%), Query Frame = 1 

Query: 1   MVSEAKGK--IPSIDLSKIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIF 60
           MV EAK K  IP IDLSK+E G KE WGLAC+EMR GLE+FGCFSVVYD+VSLELHNSIF
Sbjct: 1   MVCEAKSKVVIPIIDLSKLEEGTKE-WGLACREMRFGLEQFGCFSVVYDKVSLELHNSIF 60

Query: 61  EATKQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPA 120
           EATK+LFHLPIETRMKNTSEKPYHGYFGGYS LPLYESM IDNPI+LQ   +FANLMWP 
Sbjct: 61  EATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPT 120

Query: 121 TENNRFCETVERFSTLVAKLEKMVTKMVMESYGVS-KVVSESNMESTDYLFRCYKYRVPE 180
           TENN FC  VERFSTLVAKLE MVTKMV+ESYGVS KVVSE  MEST+YL R +KYRV E
Sbjct: 121 TENNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAE 180

Query: 181 KDESDVGLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWS 240
           KDE+DVGLHSHTDLTF SIIHQHLISGLQIQ LDDQW+++HPS+HCSF VMAGDALMAWS
Sbjct: 181 KDENDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWS 240

Query: 241 NERIRSCRHQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDK 300
           NERIRSCRH+VIM+GEETRYSIG+FSFK+  I VP+EFVDDANPLRYKPF+HYDFLTYDK
Sbjct: 241 NERIRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDK 300

Query: 301 AKASHQNVSRIKDYCGI 315
           A ASH+ +SRIKDYCG+
Sbjct: 301 ANASHKTISRIKDYCGL 316

[Close]
BLAST of Lsi02G027540 vs. NCBI nr
Match: gi|700190086|gb|KGN45319.1| (hypothetical protein Csa_7G434960 [Cucumis sativus])

HSP 1 Score: 392.5 bits (1007), Expect = 6.9e-106
Identity = 194/242 (80.17%), Postives = 212/242 (87.60%), Query Frame = 1 

Query: 1   MVSEAKGK--IPSIDLSKIEGGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIF 60
           MV EAK K  IP IDLSK+E G KE WGLAC+EMR GLE+FGCFSVVYD+VSLELHNSIF
Sbjct: 1   MVCEAKSKVVIPIIDLSKLEEGTKE-WGLACREMRFGLEQFGCFSVVYDKVSLELHNSIF 60

Query: 61  EATKQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPA 120
           EATK+LFHLPIETRMKNTSEKPYHGYFGGYS LPLYESM IDNPI+LQ   +FANLMWP 
Sbjct: 61  EATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPT 120

Query: 121 TENNRFCETVERFSTLVAKLEKMVTKMVMESYGVS-KVVSESNMESTDYLFRCYKYRVPE 180
           TENN FC  VERFSTLVAKLE MVTKMV+ESYGVS KVVSE  MEST+YL R +KYRV E
Sbjct: 121 TENNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAE 180

Query: 181 KDESDVGLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWS 240
           KDE+DVGLHSHTDLTF SIIHQHLISGLQIQ LDDQW+++HPS+HCSF VMAGDALM  +
Sbjct: 181 KDENDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMVTT 240

[Close]
BLAST of Lsi02G027540 vs. NCBI nr
Match: gi|470134199|ref|XP_004302943.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Fragaria vesca subsp. vesca])

HSP 1 Score: 360.9 bits (925), Expect = 2.2e-96
Identity = 169/313 (53.99%), Postives = 231/313 (73.80%), Query Frame = 1 

Query: 3   SEAKGKIPSIDLSKIE-GGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFEAT 62
           ++   K+P +DLSK     G + W LA KE++  LEE+GCF  +Y++V LELHNS F   
Sbjct: 5   TQPAAKVPIVDLSKENVKPGTDAWLLASKEIKYALEEYGCFEAIYNKVPLELHNSTFSVL 64

Query: 63  KQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPATEN 122
           + LF+LP+ET+M++TS++PYH YFG YS LPLYES+G+DNP +++ +  F ++MWP   N
Sbjct: 65  QDLFNLPLETKMQSTSDRPYHSYFGQYSFLPLYESLGVDNPTKIEGVQRFTSIMWPEG-N 124

Query: 123 NRFCETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKDES 182
           ++FCE+   +S LVA+L++MVTKMV E+YGV ++  ES+M ST YL RC+ YR P+ DE+
Sbjct: 125 DKFCESAHSYSKLVAELDEMVTKMVFENYGVERLY-ESHMASTTYLLRCFYYREPQLDET 184

Query: 183 DVGLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNERI 242
           D+GLH HTD TF SI+HQ  I+GLQI+S + QWID+ PS   SF+VMAGDA MAWSN+R+
Sbjct: 185 DMGLHPHTDKTFISILHQDEINGLQIKSKEGQWIDVEPSP-SSFIVMAGDAFMAWSNDRV 244

Query: 243 RSCRHQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAKAS 302
           RSC HQVIMK ++TRYSIGLF+F    + VP E VD+ +PL YKPF+H  FL ++K    
Sbjct: 245 RSCEHQVIMKEKKTRYSIGLFAFNG-VLQVPAELVDEKHPLLYKPFDHIGFLLFNKTPEG 304

Query: 303 HQNVSRIKDYCGI 315
            ++   IKDYCG+
Sbjct: 305 MKSKCPIKDYCGV 313

[Close]
BLAST of Lsi02G027540 vs. NCBI nr
Match: gi|657971607|ref|XP_008377592.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Malus domestica])

HSP 1 Score: 357.1 bits (915), Expect = 3.2e-95
Identity = 168/315 (53.33%), Postives = 228/315 (72.38%), Query Frame = 1 

Query: 1   MVSEAKGKIPSIDLSKIE-GGGKEEWGLACKEMRIGLEEFGCFSVVYDEVSLELHNSIFE 60
           M SE + K+P IDLS      G + W LACKE++  LEE+GCF  +Y ++ LELHNSIF 
Sbjct: 1   MGSETQPKLPVIDLSDENLKPGTDAWLLACKEIKDALEEYGCFEAIYHKIPLELHNSIFS 60

Query: 61  ATKQLFHLPIETRMKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQAIHNFANLMWPAT 120
           A + LF LP+ET+ + TS++PYH YFG YS LPLYES+G+DNP  ++    F ++MWP  
Sbjct: 61  AMEDLFGLPLETKKQKTSDRPYHSYFGQYSFLPLYESLGVDNPTTVEGAQGFTSIMWP-Q 120

Query: 121 ENNRFCETVERFSTLVAKLEKMVTKMVMESYGVSKVVSESNMESTDYLFRCYKYRVPEKD 180
            N  FCET   FS LVA+L +MVT+MV + YGV ++  +S+MEST YL RC+KYR  E++
Sbjct: 121 GNAHFCETAHSFSKLVAELSQMVTRMVFDMYGVQRLF-DSHMESTTYLLRCFKYRTQEEN 180

Query: 181 ESDVGLHSHTDLTFFSIIHQHLISGLQIQSLDDQWIDIHPSTHCSFVVMAGDALMAWSNE 240
           E++VGLH HTD TF SI++Q+ ++GLQI + D QWID++PS   SF+++AGDA MAWSN+
Sbjct: 181 ETNVGLHPHTDKTFISILNQNQVNGLQIGTKDGQWIDVNPSP-SSFLILAGDAFMAWSND 240

Query: 241 RIRSCRHQVIMKGEETRYSIGLFSFKSETIAVPEEFVDDANPLRYKPFNHYDFLTYDKAK 300
           R+ SC HQV MK  +TRYS+GLF+FKS  I VPEE +D+ +PL YKPF+H+ FL +D+  
Sbjct: 241 RVPSCDHQVTMKENKTRYSLGLFAFKSGIIQVPEELIDEKHPLLYKPFDHFSFLDFDRTP 300

Query: 301 ASHQNVSRIKDYCGI 315
            + +    IK YCG+
Sbjct: 301 EARKLKCSIKAYCGV 312

The following BLAST results are available for this feature:
BLAST of Lsi02G027540 vs. Swiss-Prot
Match NameE-valueIdentityDescription
AOP1L_ARATH8.3e-5939.87Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana GN=... [more]
AOP1C_ARATH3.1e-5839.40Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana GN=AO... [more]
AOP1V_ARATH7.0e-5839.67Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
AOP2V_ARATH1.7e-2734.952-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 S... [more]
AOP2L_ARATH2.9e-2734.952-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 S... [more]
BLAST of Lsi02G027540 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K8Y2_CUCSA4.8e-10680.17Uncharacterized protein OS=Cucumis sativus GN=Csa_7G434960 PE=4 SV=1[more]
M5WSX6_PRUPE3.6e-9353.33Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008973mg PE=3 SV=1[more]
W9SCZ0_9ROSA4.2e-8652.36Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis GN=L484_019468 PE=3 SV=1[more]
V4ST91_9ROSI4.7e-8550.79Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027420mg PE=3 SV=1[more]
V7APY1_PHAVU1.8e-8446.01Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_010G152400g PE=3 SV=1[more]
BLAST of Lsi02G027540 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G52800.12.3e-7544.34 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G52790.19.4e-6941.56 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G52820.16.1e-6840.00 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G15540.12.7e-6041.72 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G03070.11.8e-5939.40 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
BLAST of Lsi02G027540 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659122281|ref|XP_008461057.1|4.9e-15282.97PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumi... [more]
gi|449436585|ref|XP_004136073.1|1.0e-14982.02PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus][more]
gi|700190086|gb|KGN45319.1|6.9e-10680.17hypothetical protein Csa_7G434960 [Cucumis sativus][more]
gi|470134199|ref|XP_004302943.1|2.2e-9653.99PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Fragaria vesca su... [more]
gi|657971607|ref|XP_008377592.1|3.2e-9553.33PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Malus domestica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: INTERPRO
TermDefinition
IPR027443IPNS-like
IPR026992DIOX_N
IPR005123Oxoglu/Fe-dep_dioxygenase
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016491 oxidoreductase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G027540.1Lsi02G027540.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 171..264
score: 1.1
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 163..267
score:
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 9..86
score: 2.3
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 6..295
score: 1.8
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 3..308
score: 1.1E
NoneNo IPR availablePANTHERPTHR10209:SF182SUBFAMILY NOT NAMEDcoord: 3..308
score: 1.1E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 8..295
score: 6.11

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi02G027540Bhi09G000065Wax gourdlsiwgoB270
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Lsi02G027540Bottle gourd (USVL1VR-Ls)lsilsiB052
Lsi02G027540Bottle gourd (USVL1VR-Ls)lsilsiB117
Lsi02G027540Bottle gourd (USVL1VR-Ls)lsilsiB124
Lsi02G027540Cucumber (Gy14) v1cgylsiB050
Lsi02G027540Cucumber (Gy14) v1cgylsiB260
Lsi02G027540Cucurbita maxima (Rimu)cmalsiB051
Lsi02G027540Cucurbita maxima (Rimu)cmalsiB238
Lsi02G027540Cucurbita maxima (Rimu)cmalsiB348
Lsi02G027540Cucurbita maxima (Rimu)cmalsiB418
Lsi02G027540Cucurbita maxima (Rimu)cmalsiB827
Lsi02G027540Cucurbita moschata (Rifu)cmolsiB037
Lsi02G027540Cucurbita moschata (Rifu)cmolsiB225
Lsi02G027540Cucurbita moschata (Rifu)cmolsiB337
Lsi02G027540Cucurbita moschata (Rifu)cmolsiB405
Lsi02G027540Cucurbita moschata (Rifu)cmolsiB810
Lsi02G027540Cucurbita moschata (Rifu)cmolsiB814
Lsi02G027540Cucurbita pepo (Zucchini)cpelsiB113
Lsi02G027540Cucurbita pepo (Zucchini)cpelsiB259
Lsi02G027540Cucurbita pepo (Zucchini)cpelsiB442
Lsi02G027540Wild cucumber (PI 183967)cpilsiB112
Lsi02G027540Wild cucumber (PI 183967)cpilsiB517
Lsi02G027540Cucumber (Chinese Long) v2culsiB114
Lsi02G027540Cucumber (Chinese Long) v2culsiB503
Lsi02G027540Melon (DHL92) v3.5.1lsimeB185
Lsi02G027540Melon (DHL92) v3.5.1lsimeB211
Lsi02G027540Melon (DHL92) v3.5.1lsimeB213
Lsi02G027540Watermelon (Charleston Gray)lsiwcgB160
Lsi02G027540Watermelon (Charleston Gray)lsiwcgB204
Lsi02G027540Watermelon (Charleston Gray)lsiwcgB210
Lsi02G027540Watermelon (97103) v1lsiwmB176
Lsi02G027540Watermelon (97103) v1lsiwmB183
Lsi02G027540Watermelon (97103) v1lsiwmB191
Lsi02G027540Cucumber (Gy14) v2cgyblsiB101
Lsi02G027540Cucumber (Gy14) v2cgyblsiB472
Lsi02G027540Melon (DHL92) v3.6.1lsimedB194
Lsi02G027540Melon (DHL92) v3.6.1lsimedB220
Lsi02G027540Melon (DHL92) v3.6.1lsimedB222
Lsi02G027540Melon (DHL92) v3.6.1lsimedB229
Lsi02G027540Silver-seed gourdcarlsiB106
Lsi02G027540Silver-seed gourdcarlsiB114
Lsi02G027540Silver-seed gourdcarlsiB131
Lsi02G027540Silver-seed gourdcarlsiB176
Lsi02G027540Silver-seed gourdcarlsiB516
Lsi02G027540Silver-seed gourdcarlsiB937
Lsi02G027540Cucumber (Chinese Long) v3cuclsiB124
Lsi02G027540Cucumber (Chinese Long) v3cuclsiB126
Lsi02G027540Cucumber (Chinese Long) v3cuclsiB538
Lsi02G027540Watermelon (97103) v2lsiwmbB170
Lsi02G027540Watermelon (97103) v2lsiwmbB199
Lsi02G027540Watermelon (97103) v2lsiwmbB206
Lsi02G027540Watermelon (97103) v2lsiwmbB213
Lsi02G027540Wax gourdlsiwgoB221
Lsi02G027540Wax gourdlsiwgoB225
Lsi02G027540Wax gourdlsiwgoB286