Lsi10G008400 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi10G008400
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionSEC1 family transport protein SLY1
Locationchr10 : 11977752 .. 11980306 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACCCACCGTCGTATTTCGAGAACTTTGATTCGGCGAATTGAAAGAACGAATCCTCCAAAACCGAAGCTTTCGATTCCCACTGGAAAACAAGGGGACTGAATTTTACTGGGCTACGAATCGCCATGGCTTTGAATCTTCGCCAGAAGCAAACAGGTAATTTCTCTCTGTCTCTCTAGATCCCAGTTTTGAGGAGTGATCCAAACGAAATTTGGTGCTACATTGATGTTTTAAAAGTGGGATTTGTTTGTGGGTTTGATTTAAACTTCTTGCGATTGCAGAATGTATAATTCGAATGTTGAATTTGAACCAGCCGGTGAACTCAACATCGACCGGGAACGAAGAAGAGGTTTACAAGATCTTGATCTTCGACAGATTCTGCCAGAACATACTGTCCCCTTTGATTCATGTCAAGGATCTTCGCAAACATGGGATCACTCTTTATTTTCTCATCGACAAGGATCGAAAACCTGTTCACGATGTGCCCGCGGTCTATTTCGTCCAACCGAGTAAGATCAACATAGATAGAATTGTTGCTGATGCTTCTCGTTTCCTCTACGATAGCTTTTATTTAAACTTCTCTTCTTCTATTCCTCGTCCTCTTCTTGAGGATCTCGCATCTGGGTCGCTGAATTCGGATTCGGTACAACGAATCGCGAAGGTTCACGACCAGTATTTGGAGTTCGTTACATTAGAAGATAATTTGTTTTCGTTAGCACAGAAGAGTATATATGTCCAATTGAACGATCCCTCTGCTGGAGATCGTGAAATTGAGGAGATTATAGAGAGAATCGTGAGTGGGTTGTTTAGTGTTTTGGCTACGCTTGCTGTAGTGCCTGTTATTCGATGCCAACGAGGAGGGCCGGCGGAAATGGTTGCCTCGGCATTGGATCAGAGGTTGAGAGATCATTTTTTGTCGAAGAACAATTTGTTTACAGAGGGTGGGGGATTTGCTAGCTCCTTCCAGCGTCCGATTCTGTGTCTGTTTGATCGAAATTTTGAGTTATCTGTAGGAATACAGCATGATTTTAGGTACAGACCTTTAGTTCATGATGTTCTAGGATTGAAGTTGAATAGATTGAGTGTGCAGGGTGAAAAGGGTGGGATGAAATCATATGAATTAGATAGTTCAGACCCATTTTGGGTGGCAAATGGGTCTTTGGAGTTTCCAGAAGTTGCAGTTGAGATTGAGACTCAGTTGAATAAGTATAAGAGGGATGTTGATGAGGTGAATAGAAGAACTGGTGGGACTGATGGAGCTGAGTTTGATGGAACAGATTTGATTGGGAACACAAAACATTTGATGAATGCAGTGAACTCATTGCCAGAATTGACTGAAAGGAAGCAAGTTATTGATAAGCATACAAACATTGCTACGGTATTGTTGGGTGAGATCAAGGAGAGGTCACTTGATTCATATGCTAAAAAGGAGAATGACATGATGGCCAGAGGAGGGATCGATCGGAACGAACTCTTGAGTGTACTCAAAGGGAAGGGTACTAAGACGGATAAGCTCCGATTTGCTGTTATATATCTTATCTCTTCTGAAACTATAAACCAATCTGAAGTGGAAGCTGTGGAAACTGCTCTGAGGGAATCAGAGGTTGATACTAGTGCATTTCAATATGTCAAGAAGATCAAGTCATTGAATGTCTCATTCTCATCAGCAAATTCAGCTAGCAGAAGTAACATTGTGGATTGGGCTGAAAAACTCTATGGACAGTCAATTAGTGCAGTGACTGCTGGTGTTAAAAATCTTTTGTCTAGTGATAGGCAGCTTGCACTGACTAGGACGGTAGAGGGGTTGATGGAAGGAAAACTGAATCCTGAAATTGATACTTTCCTCACATTCGATCCACGTGCTCCAAAGTCGAGTTCTGGAACAAGTAGCAGCCATTTGAAAGGACCATTTAAGGAGGCAATTGTGTTCATGATTGGAGGGGGTAACTATGTCGAGTATGGGAGCTTGCAAGAGCTGGCAATGAATCAACAGCCAGTCAAGCATATAATATACGGTAGCACCGAAATCCTCACTGGAGTTGAGTTTGTGGAACAGCTTTTGCTTTTGGGGCAGAAGATGGGACTTGGCAATGTTGCTGCTGCTCCTCCTCCTCCAGGTCGTTAACTGAACTCCCTTCTGTAGAATTTCTTTGTCCATTGCAGTACCTTAGATTGGCTAATTCATTAAAGAAATTGTTGCATATCTGAAACAATTCTGGACAAGAACATTTTGGGTTATAGAATCTACCCACCTCAGTCATTTCGAGGCGGCTTCGTGTTCTTGAAATCAGACTCTTGATTGGCCTCAGCCTCCGATAATCTGGGAAGAATGCCTTTTTGTAGATTTTAGCTTGTCCATTTTAATAATTTTTTGTATTCTTTAGCAGTTTTATGTATTTGGAAATTCACTTGCCAAACATTCTTTTGTCAAGCTTGAGTCCTAAATTTCTTTGTCAAATGATATCTACAAAGATTATATGTGTAGTCGTAGGTCTTTTCAATTCTCTTGGTTGCTATTCACTTTCCTAAAACCAGCTTGCTTCATTTTAATTTTTGA

mRNA sequence

AACCCACCGTCGTATTTCGAGAACTTTGATTCGGCGAATTGAAAGAACGAATCCTCCAAAACCGAAGCTTTCGATTCCCACTGGAAAACAAGGGGACTGAATTTTACTGGGCTACGAATCGCCATGGCTTTGAATCTTCGCCAGAAGCAAACAGAATGTATAATTCGAATGTTGAATTTGAACCAGCCGGTGAACTCAACATCGACCGGGAACGAAGAAGAGGTTTACAAGATCTTGATCTTCGACAGATTCTGCCAGAACATACTGTCCCCTTTGATTCATGTCAAGGATCTTCGCAAACATGGGATCACTCTTTATTTTCTCATCGACAAGGATCGAAAACCTGTTCACGATGTGCCCGCGGTCTATTTCGTCCAACCGAGTAAGATCAACATAGATAGAATTGTTGCTGATGCTTCTCGTTTCCTCTACGATAGCTTTTATTTAAACTTCTCTTCTTCTATTCCTCGTCCTCTTCTTGAGGATCTCGCATCTGGGTCGCTGAATTCGGATTCGGTACAACGAATCGCGAAGGTTCACGACCAGTATTTGGAGTTCGTTACATTAGAAGATAATTTGTTTTCGTTAGCACAGAAGAGTATATATGTCCAATTGAACGATCCCTCTGCTGGAGATCGTGAAATTGAGGAGATTATAGAGAGAATCGTGAGTGGGTTGTTTAGTGTTTTGGCTACGCTTGCTGTAGTGCCTGTTATTCGATGCCAACGAGGAGGGCCGGCGGAAATGGTTGCCTCGGCATTGGATCAGAGGTTGAGAGATCATTTTTTGTCGAAGAACAATTTGTTTACAGAGGGTGGGGGATTTGCTAGCTCCTTCCAGCGTCCGATTCTGTGTCTGTTTGATCGAAATTTTGAGTTATCTGTAGGAATACAGCATGATTTTAGGTACAGACCTTTAGTTCATGATGTTCTAGGATTGAAGTTGAATAGATTGAGTGTGCAGGGTGAAAAGGGTGGGATGAAATCATATGAATTAGATAGTTCAGACCCATTTTGGGTGGCAAATGGGTCTTTGGAGTTTCCAGAAGTTGCAGTTGAGATTGAGACTCAGTTGAATAAGTATAAGAGGGATGTTGATGAGGTGAATAGAAGAACTGGTGGGACTGATGGAGCTGAGTTTGATGGAACAGATTTGATTGGGAACACAAAACATTTGATGAATGCAGTGAACTCATTGCCAGAATTGACTGAAAGGAAGCAAGTTATTGATAAGCATACAAACATTGCTACGGTATTGTTGGGTGAGATCAAGGAGAGGTCACTTGATTCATATGCTAAAAAGGAGAATGACATGATGGCCAGAGGAGGGATCGATCGGAACGAACTCTTGAGTGTACTCAAAGGGAAGGGTACTAAGACGGATAAGCTCCGATTTGCTGTTATATATCTTATCTCTTCTGAAACTATAAACCAATCTGAAGTGGAAGCTGTGGAAACTGCTCTGAGGGAATCAGAGGTTGATACTAGTGCATTTCAATATGTCAAGAAGATCAAGTCATTGAATGTCTCATTCTCATCAGCAAATTCAGCTAGCAGAAGTAACATTGTGGATTGGGCTGAAAAACTCTATGGACAGTCAATTAGTGCAGTGACTGCTGGTGTTAAAAATCTTTTGTCTAGTGATAGGCAGCTTGCACTGACTAGGACGGTAGAGGGGTTGATGGAAGGAAAACTGAATCCTGAAATTGATACTTTCCTCACATTCGATCCACGTGCTCCAAAGTCGAGTTCTGGAACAAGTAGCAGCCATTTGAAAGGACCATTTAAGGAGGCAATTGTGTTCATGATTGGAGGGGGTAACTATGTCGAGTATGGGAGCTTGCAAGAGCTGGCAATGAATCAACAGCCAGTCAAGCATATAATATACGGTAGCACCGAAATCCTCACTGGAGTTGAGTTTGTGGAACAGCTTTTGCTTTTGGGGCAGAAGATGGGACTTGGCAATGTTGCTGCTGCTCCTCCTCCTCCAGGTCGTTAACTGAACTCCCTTCTGTAGAATTTCTTTGTCCATTGCAGTACCTTAGATTGGCTAATTCATTAAAGAAATTGTTGCATATCTGAAACAATTCTGGACAAGAACATTTTGGGTTATAGAATCTACCCACCTCAGTCATTTCGAGGCGGCTTCGTGTTCTTGAAATCAGACTCTTGATTGGCCTCAGCCTCCGATAATCTGGGAAGAATGCCTTTTTGTAGATTTTAGCTTGTCCATTTTAATAATTTTTTGTATTCTTTAGCAGTTTTATGTATTTGGAAATTCACTTGCCAAACATTCTTTTGTCAAGCTTGAGTCCTAAATTTCTTTGTCAAATGATATCTACAAAGATTATATGTGTAGTCGTAGGTCTTTTCAATTCTCTTGGTTGCTATTCACTTTCCTAAAACCAGCTTGCTTCATTTTAATTTTTGA

Coding sequence (CDS)

ATGGCTTTGAATCTTCGCCAGAAGCAAACAGAATGTATAATTCGAATGTTGAATTTGAACCAGCCGGTGAACTCAACATCGACCGGGAACGAAGAAGAGGTTTACAAGATCTTGATCTTCGACAGATTCTGCCAGAACATACTGTCCCCTTTGATTCATGTCAAGGATCTTCGCAAACATGGGATCACTCTTTATTTTCTCATCGACAAGGATCGAAAACCTGTTCACGATGTGCCCGCGGTCTATTTCGTCCAACCGAGTAAGATCAACATAGATAGAATTGTTGCTGATGCTTCTCGTTTCCTCTACGATAGCTTTTATTTAAACTTCTCTTCTTCTATTCCTCGTCCTCTTCTTGAGGATCTCGCATCTGGGTCGCTGAATTCGGATTCGGTACAACGAATCGCGAAGGTTCACGACCAGTATTTGGAGTTCGTTACATTAGAAGATAATTTGTTTTCGTTAGCACAGAAGAGTATATATGTCCAATTGAACGATCCCTCTGCTGGAGATCGTGAAATTGAGGAGATTATAGAGAGAATCGTGAGTGGGTTGTTTAGTGTTTTGGCTACGCTTGCTGTAGTGCCTGTTATTCGATGCCAACGAGGAGGGCCGGCGGAAATGGTTGCCTCGGCATTGGATCAGAGGTTGAGAGATCATTTTTTGTCGAAGAACAATTTGTTTACAGAGGGTGGGGGATTTGCTAGCTCCTTCCAGCGTCCGATTCTGTGTCTGTTTGATCGAAATTTTGAGTTATCTGTAGGAATACAGCATGATTTTAGGTACAGACCTTTAGTTCATGATGTTCTAGGATTGAAGTTGAATAGATTGAGTGTGCAGGGTGAAAAGGGTGGGATGAAATCATATGAATTAGATAGTTCAGACCCATTTTGGGTGGCAAATGGGTCTTTGGAGTTTCCAGAAGTTGCAGTTGAGATTGAGACTCAGTTGAATAAGTATAAGAGGGATGTTGATGAGGTGAATAGAAGAACTGGTGGGACTGATGGAGCTGAGTTTGATGGAACAGATTTGATTGGGAACACAAAACATTTGATGAATGCAGTGAACTCATTGCCAGAATTGACTGAAAGGAAGCAAGTTATTGATAAGCATACAAACATTGCTACGGTATTGTTGGGTGAGATCAAGGAGAGGTCACTTGATTCATATGCTAAAAAGGAGAATGACATGATGGCCAGAGGAGGGATCGATCGGAACGAACTCTTGAGTGTACTCAAAGGGAAGGGTACTAAGACGGATAAGCTCCGATTTGCTGTTATATATCTTATCTCTTCTGAAACTATAAACCAATCTGAAGTGGAAGCTGTGGAAACTGCTCTGAGGGAATCAGAGGTTGATACTAGTGCATTTCAATATGTCAAGAAGATCAAGTCATTGAATGTCTCATTCTCATCAGCAAATTCAGCTAGCAGAAGTAACATTGTGGATTGGGCTGAAAAACTCTATGGACAGTCAATTAGTGCAGTGACTGCTGGTGTTAAAAATCTTTTGTCTAGTGATAGGCAGCTTGCACTGACTAGGACGGTAGAGGGGTTGATGGAAGGAAAACTGAATCCTGAAATTGATACTTTCCTCACATTCGATCCACGTGCTCCAAAGTCGAGTTCTGGAACAAGTAGCAGCCATTTGAAAGGACCATTTAAGGAGGCAATTGTGTTCATGATTGGAGGGGGTAACTATGTCGAGTATGGGAGCTTGCAAGAGCTGGCAATGAATCAACAGCCAGTCAAGCATATAATATACGGTAGCACCGAAATCCTCACTGGAGTTGAGTTTGTGGAACAGCTTTTGCTTTTGGGGCAGAAGATGGGACTTGGCAATGTTGCTGCTGCTCCTCCTCCTCCAGGTCGTTAA

Protein sequence

MALNLRQKQTECIIRMLNLNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKHGITLYFLIDKDRKPVHDVPAVYFVQPSKINIDRIVADASRFLYDSFYLNFSSSIPRPLLEDLASGSLNSDSVQRIAKVHDQYLEFVTLEDNLFSLAQKSIYVQLNDPSAGDREIEEIIERIVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHFLSKNNLFTEGGGFASSFQRPILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLSVQGEKGGMKSYELDSSDPFWVANGSLEFPEVAVEIETQLNKYKRDVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPELTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGKGTKTDKLRFAVIYLISSETINQSEVEAVETALRESEVDTSAFQYVKKIKSLNVSFSSANSASRSNIVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMEGKLNPEIDTFLTFDPRAPKSSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELAMNQQPVKHIIYGSTEILTGVEFVEQLLLLGQKMGLGNVAAAPPPPGR
BLAST of Lsi10G008400 vs. Swiss-Prot
Match: SLY1_ARATH (SEC1 family transport protein SLY1 OS=Arabidopsis thaliana GN=SLY1 PE=1 SV=1)

HSP 1 Score: 978.4 bits (2528), Expect = 3.7e-284
Identity = 495/622 (79.58%), Postives = 555/622 (89.23%), Query Frame = 1

Query: 1   MALNLRQKQTECIIRMLNLNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKH 60
           MALNLRQKQTEC+IRMLNLNQP+N + T NEE VYKILI+DRFCQNILSPL HVKDLRKH
Sbjct: 1   MALNLRQKQTECVIRMLNLNQPLNPSGTANEE-VYKILIYDRFCQNILSPLTHVKDLRKH 60

Query: 61  GITLYFLIDKDRKPVHDVPAVYFVQPSKINIDRIVADASRFLYDSFYLNFSSSIPRPLLE 120
           G+TL+FLIDKDR+PVHDVPAVYFVQP++ N+ RI+ADASR LYD+F+LNFSSSIPR  LE
Sbjct: 61  GVTLFFLIDKDRQPVHDVPAVYFVQPTESNLQRIIADASRSLYDTFHLNFSSSIPRKFLE 120

Query: 121 DLASGSLNSDSVQRIAKVHDQYLEFVTLEDNLFSLAQKSIYVQLNDPSAGDREIEEIIER 180
           +LASG+L S SV++++KVHDQYLEFVTLEDNLFSLAQ+S YVQ+NDPSAG++EI EIIER
Sbjct: 121 ELASGTLKSGSVEKVSKVHDQYLEFVTLEDNLFSLAQQSTYVQMNDPSAGEKEINEIIER 180

Query: 181 IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHFLSKNNLFTEGGGFASSFQR 240
           + SGLF VL TL VVPVIRC  GGPAEMVAS LDQ+LRDH LSKNNLFTEGGGF SSFQR
Sbjct: 181 VASGLFCVLVTLGVVPVIRCPSGGPAEMVASLLDQKLRDHLLSKNNLFTEGGGFMSSFQR 240

Query: 241 PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLSVQGEKGGMKSYELDSSDPFWVA 300
           P+LC+FDRNFELSVGIQHDFRYRPLVHDVLGLKLN+L VQGEKG  KS+ELDSSDPFW A
Sbjct: 241 PLLCIFDRNFELSVGIQHDFRYRPLVHDVLGLKLNQLKVQGEKGPPKSFELDSSDPFWSA 300

Query: 301 NGSLEFPEVAVEIETQLNKYKRDVDEVNRRTGGTDGAEFDGTDLIGN--TKHLMNAVNSL 360
           N +LEFP+VAVEIETQLNKYKRDV+EVN++TGG  GAEFDGTDLIGN  T+HLMN V SL
Sbjct: 301 NSTLEFPDVAVEIETQLNKYKRDVEEVNKKTGGGSGAEFDGTDLIGNIHTEHLMNTVKSL 360

Query: 361 PELTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGKGTK 420
           PELTERK+VIDKHTNIAT LLG+IKERS+D++ KKE+DMM RGGIDR EL++ LKGKGTK
Sbjct: 361 PELTERKKVIDKHTNIATALLGQIKERSIDAFTKKESDMMMRGGIDRTELMAALKGKGTK 420

Query: 421 TDKLRFAVIYLISSETINQSEVEAVETALRESEVDTSAFQYVKKIKSLNVSF--SSANSA 480
            DKLRFA++YLIS+ETINQSEVEAVE AL E+E DTSAFQYVKKIKSLN SF  +SANSA
Sbjct: 421 MDKLRFAIMYLISTETINQSEVEAVEAALNEAEADTSAFQYVKKIKSLNASFAATSANSA 480

Query: 481 SRSNIVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMEGKLNPEIDTFLTFDP 540
           SRSNIVDWAEKLYGQSISAVTAGVKNLLSSD+QLA+TRTVE L EGK NPEID++   DP
Sbjct: 481 SRSNIVDWAEKLYGQSISAVTAGVKNLLSSDQQLAVTRTVEALTEGKPNPEIDSYRFLDP 540

Query: 541 RAPKSSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELAMNQQPVKHIIYGSTEILTG 600
           RAPKSSS +  SH+KGPF+EAIVFMIGGGNYVEYGSLQEL   Q  VK++IYG+TEIL G
Sbjct: 541 RAPKSSS-SGGSHVKGPFREAIVFMIGGGNYVEYGSLQELTQRQLTVKNVIYGATEILNG 600

Query: 601 VEFVEQLLLLGQKMGLGNVAAA 619
            E VEQL LLG+KMGLG   A+
Sbjct: 601 GELVEQLGLLGKKMGLGGPVAS 620

BLAST of Lsi10G008400 vs. Swiss-Prot
Match: SLY1_ORYSJ (SEC1 family transport protein SLY1 OS=Oryza sativa subsp. japonica GN=SLY1 PE=2 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 6.2e-215
Identity = 392/632 (62.03%), Postives = 479/632 (75.79%), Query Frame = 1

Query: 1   MALNLRQKQTECIIRMLNLNQPVNSTSTG--------NEEEVYKILIFDRFCQNILSPLI 60
           MAL LR+KQ + I+RML+LNQ +  +  G         EEE YKIL+ D  C  +L+P++
Sbjct: 1   MALTLRKKQLDSIVRMLHLNQQLQGSPDGVGGGVGSAEEEEAYKILVMDSPCVALLAPVL 60

Query: 61  HVKDLRKHGITLYFLIDKDRKPVHDVPAVYFVQPSKINIDRIVADASRFLYDSFYLNFSS 120
            V +LR+HG+TL+  IDK R+ V D PAVY ++P+  N+DR+ ADA+  LY SF+LNFS+
Sbjct: 61  RVGELRRHGVTLHLNIDKARQQVPDAPAVYLLRPTAANVDRVAADAAAGLYASFHLNFST 120

Query: 121 SIPRPLLEDLASGSLNSDSVQRIAKVHDQYLEFVTLEDNLFSLAQKSIYVQLNDPSAGDR 180
            +PR LLE LAS +  S S  R+A+V DQYL+FV LE+ LFSLAQ   YV LNDP+A + 
Sbjct: 121 CVPRALLERLASATAASRSAHRVARVADQYLDFVCLEEGLFSLAQPRAYVALNDPAAAEA 180

Query: 181 EIEEIIERIVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHFLSKNNLFTEGG 240
           +I  +++ I  GLF V+ATL  VPVIRC  GGPAEMVA+ALD RLRDH ++K NLFTE  
Sbjct: 181 DITALVDAIALGLFCVVATLGAVPVIRCAGGGPAEMVAAALDARLRDHLIAKPNLFTEAA 240

Query: 241 GFA-SSFQRPILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLSVQGEKGGMKSYEL 300
             A +SFQRP+LCLFDRNFELSVGIQHD+ YRPLVHDVLGLK N+L +       + Y+L
Sbjct: 241 STAVASFQRPLLCLFDRNFELSVGIQHDWSYRPLVHDVLGLKSNKLKLP------EKYDL 300

Query: 301 DSSDPFWVANGSLEFPEVAVEIETQLNKYKRDVDEVNRRT-GGTDGAEFDGTDLIGNTKH 360
           D +DPFWVAN  L+FP+VA EIE QL KYK+DVDEVN+RT GG DG EFDGTDLIGNT+H
Sbjct: 301 DDTDPFWVANSWLQFPKVAEEIEAQLAKYKQDVDEVNQRTGGGRDGVEFDGTDLIGNTRH 360

Query: 361 LMNAVNSLPELTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLS 420
           LMNAVNSLPELTERK++IDKHTNIAT LLG IK RSLD Y + EN M+  G +DR +L++
Sbjct: 361 LMNAVNSLPELTERKKMIDKHTNIATALLGHIKGRSLDGYFECENSMLVDGTLDRTKLMN 420

Query: 421 VLKGKGTKTDKLRFAVIYLISSETINQSEVEAVETALRESEVDTSAFQYVKKIKSLNVSF 480
           +L+G GTK DKLR AV YL+S ET   S++E VE ALRESEVD SAFQYVK+IKSLN  F
Sbjct: 421 LLRGNGTKEDKLRLAVTYLLSFETPVPSDLEQVEAALRESEVDMSAFQYVKRIKSLNSQF 480

Query: 481 SSA-NSASRSNIVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMEGKLNPEID 540
           + A N+AS+ NIVDWAEKLYG SISA+T GV+NLLS  +QLA TR VE LMEGK NPE+D
Sbjct: 481 AGASNTASKVNIVDWAEKLYGHSISAMT-GVRNLLSDGKQLAATRAVEALMEGKPNPEVD 540

Query: 541 TFLTFDPRAPKSSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELAMNQQPVKHIIYG 600
            +L FDPRAPKS    ++   +GPF+EAIVFMIGGGNY+EY SL EL    Q  K +IYG
Sbjct: 541 NYLLFDPRAPKSG---TAGQFRGPFREAIVFMIGGGNYIEYRSLTELTQRSQTTKQVIYG 600

Query: 601 STEILTGVEFVEQLLLLGQKMGLGNVAAAPPP 622
           +TEIL GVEF++QL  LGQK GLG V+++ PP
Sbjct: 601 ATEILNGVEFIQQLSELGQKAGLGGVSSSLPP 622

BLAST of Lsi10G008400 vs. Swiss-Prot
Match: SCFD1_HUMAN (Sec1 family domain-containing protein 1 OS=Homo sapiens GN=SCFD1 PE=1 SV=4)

HSP 1 Score: 443.7 bits (1140), Expect = 3.3e-123
Identity = 257/636 (40.41%), Postives = 387/636 (60.85%), Query Frame = 1

Query: 2   ALNLRQKQTECIIRMLNLNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKHG 61
           A ++R++QT  + RMLN N P    STG  E V+K+LI+DRF Q+I+SPL+ VK+LR  G
Sbjct: 12  AASIRERQTVALKRMLNFNVPHIKNSTG--EPVWKVLIYDRFGQDIISPLLSVKELRDMG 71

Query: 62  ITLYFLIDKDRKPVHDVPAVYFVQPSKINIDRIVADASRFLYDSFYLNFSSSIPRPLLED 121
           ITL+ L+  DR P+ DVPAVYFV P++ NIDR+  D    LY+S+YLNF S+I R  LED
Sbjct: 72  ITLHLLLHSDRDPIPDVPAVYFVMPTEENIDRMCQDLRNQLYESYYLNFISAISRSKLED 131

Query: 122 LASGSLNSDSVQRIAKVHDQYLEFVTLEDNLFSLAQKS----IYVQLNDPSAGDREIEEI 181
           +A+ +L + +V ++AKV DQYL F+TLED++F L  ++     Y  +N P   D E+E +
Sbjct: 132 IANAALAASAVTQVAKVFDQYLNFITLEDDMFVLCNQNKELVSYRAINRPDITDTEMETV 191

Query: 182 IERIVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHFL-SKNNLFTEG--GGF 241
           ++ IV  LF    TL  VP+IRC RG  AEMVA  LD++LR++   ++N+LFT    G  
Sbjct: 192 MDTIVDSLFCFFVTLGAVPIIRCSRGTAAEMVAVKLDKKLRENLRDARNSLFTGDTLGAG 251

Query: 242 ASSFQRPILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLSVQGEKG---------- 301
             SFQRP+L L DRN +L+  + H + Y+ LVHDVL   LNR++++   G          
Sbjct: 252 QFSFQRPLLVLVDRNIDLATPLHHTWTYQALVHDVLDFHLNRVNLEESSGVENSPAGARP 311

Query: 302 ---GMKSYELDSSDPFWVANGSLEFPEVAVEIETQLNKYKRDVDEVNRRTG--GTDGAEF 361
                KSY+L   D FW  +    FPEVA  ++ +L  Y+   DEV R     G +G + 
Sbjct: 312 KRKNKKSYDLTPVDKFWQKHKGSPFPEVAESVQQELESYRAQEDEVKRLKSIMGLEGEDE 371

Query: 362 DGTDLIG-NTKHLMNAVNSLPELTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMM 421
               ++  NT  L +AV+SLPEL E+K++ID HTN+AT +L  IK R LD Y + E  +M
Sbjct: 372 GAISMLSDNTAKLTSAVSSLPELLEKKRLIDLHTNVATAVLEHIKARKLDVYFEYEEKIM 431

Query: 422 ARGGIDRNELLSVLK--GKGTKTDKLRFAVIYLISSETI-NQSEVEAVETALRESEVDTS 481
           ++  +D++ LL ++     GT  DK+R  +IY IS++   +++++E  + AL ++  + +
Sbjct: 432 SKTTLDKS-LLDIISDPDAGTPEDKMRLFLIYYISTQQAPSEADLEQYKKALTDAGCNLN 491

Query: 482 AFQYVKKIKSLN--VSFSSANSASRSNIVDWAEKLYGQSISAVTAGVKNLLSSDRQLALT 541
             QY+K+ K+     S  ++  ++ +  +    ++       V  GVKNL+   + L +T
Sbjct: 492 PLQYIKQWKAFTKMASAPASYGSTTTKPMGLLSRVMNTGSQFVMEGVKNLVLKQQNLPVT 551

Query: 542 RTVEGLMEGKLNPEIDTFLTFDPRAPKSSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSL 601
           R ++ LME K NPE D +  FDP+  + +  +S    K PF+EAIVF++GGGNY+EY +L
Sbjct: 552 RILDNLMEMKSNPETDDYRYFDPKMLRGND-SSVPRNKNPFQEAIVFVVGGGNYIEYQNL 611

Query: 602 QELAMNQQPVKHIIYGSTEILTGVEFVEQLLLLGQK 610
            +    +Q  KHI+YG +E+    +F++QL  LGQK
Sbjct: 612 VDYIKGKQG-KHILYGCSELFNATQFIKQLSQLGQK 642

BLAST of Lsi10G008400 vs. Swiss-Prot
Match: SCFD1_RAT (Sec1 family domain-containing protein 1 OS=Rattus norvegicus GN=Scfd1 PE=1 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 3.3e-123
Identity = 261/637 (40.97%), Postives = 386/637 (60.60%), Query Frame = 1

Query: 1   MALNLRQKQTECIIRMLNLNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKH 60
           MA ++R++QT  + RMLN N P    S G  E V+K+LI+DRF Q+I+SPL+ VK+LR  
Sbjct: 6   MAASIRERQTVALKRMLNFNVPHVKNSPG--EPVWKVLIYDRFGQDIISPLLSVKELRDM 65

Query: 61  GITLYFLIDKDRKPVHDVPAVYFVQPSKINIDRIVADASRFLYDSFYLNFSSSIPRPLLE 120
           GITL+ L+  DR P+ DVPAVYFV P++ NIDR+  D    LY+S+YLNF S+I R  LE
Sbjct: 66  GITLHLLLHSDRDPIRDVPAVYFVMPTEENIDRLCQDLRNQLYESYYLNFISAISRSKLE 125

Query: 121 DLASGSLNSDSVQRIAKVHDQYLEFVTLEDNLFSLAQKS----IYVQLNDPSAGDREIEE 180
           D+A+ +L +++V ++AKV DQYL F+TLE+++F L  ++     Y  +N P   D E+E 
Sbjct: 126 DIANAALAANAVTQVAKVFDQYLNFITLEEDMFVLCNQNKELVSYRAINRPDITDTEMET 185

Query: 181 IIERIVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHFL-SKNNLFTEG--GG 240
           +++ IV  LF    TL  VP+IRC RG  AEMVA  LD++LR++   ++N+LFT    G 
Sbjct: 186 VMDTIVDSLFCFFVTLGAVPIIRCSRGTAAEMVAVKLDKKLRENLRDARNSLFTGDPLGT 245

Query: 241 FASSFQRPILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLSVQGEKG--------- 300
              SFQRP+L L DRN +L+  + H + Y+ LVHDVL   LNR++++   G         
Sbjct: 246 GQFSFQRPLLVLVDRNIDLATPLHHTWTYQALVHDVLDFHLNRVNLEESTGVENSPTGAR 305

Query: 301 ----GMKSYELDSSDPFWVANGSLEFPEVAVEIETQLNKYKRDVDEVNRRTG--GTDGAE 360
                 KSY+L   D FW  +    FPEVA  ++ +L  Y+   DEV R     G +G +
Sbjct: 306 PKRKNKKSYDLTPVDKFWQKHKGSPFPEVAESVQQELESYRAQEDEVKRLKSIMGLEGED 365

Query: 361 FDGTDLIG-NTKHLMNAVNSLPELTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDM 420
                ++  NT  L +AV+SLPEL E+K++ID HTN+AT +L  IK R LD Y + E  +
Sbjct: 366 EGAISMLSDNTAKLTSAVSSLPELLEKKRLIDLHTNVATAVLEHIKARKLDVYFEYEEKI 425

Query: 421 MARGGIDRNELLSVLK--GKGTKTDKLRFAVIYLISSETI-NQSEVEAVETALRESEVDT 480
           M++  +D++ LL V+     GT  DK+R  +IY IS++   ++ ++E  + AL ++  + 
Sbjct: 426 MSKTTLDKS-LLDVISDPDAGTPEDKMRLFLIYYISAQQAPSEVDLEQYKKALTDAGCNL 485

Query: 481 SAFQYVKKIKSLNVSFSSANSASRSNI--VDWAEKLYGQSISAVTAGVKNLLSSDRQLAL 540
           S  QY+K+ K+     S+  S   +    +    ++       V  GVKNL+   + L +
Sbjct: 486 SPLQYIKQWKAFAKMASTPASYGNTTTKPMGLLSRVMNTGSQFVMEGVKNLVLKQQNLPV 545

Query: 541 TRTVEGLMEGKLNPEIDTFLTFDPRAPKSSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGS 600
           TR ++ LME K NPE D +  FDP+  +S+  +S    K PF+EAIVF++GGGNY+EY +
Sbjct: 546 TRILDNLMEMKSNPETDDYRYFDPKMLRSND-SSVPRNKSPFQEAIVFVVGGGNYIEYQN 605

Query: 601 LQELAMNQQPVKHIIYGSTEILTGVEFVEQLLLLGQK 610
           L +    +Q  KHI+YG +EI    +F++QL  LGQK
Sbjct: 606 LVDYIKGKQG-KHILYGCSEIFNATQFIKQLSQLGQK 637

BLAST of Lsi10G008400 vs. Swiss-Prot
Match: SCFD1_MOUSE (Sec1 family domain-containing protein 1 OS=Mus musculus GN=Scfd1 PE=1 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 7.2e-123
Identity = 261/634 (41.17%), Postives = 384/634 (60.57%), Query Frame = 1

Query: 4   NLRQKQTECIIRMLNLNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKHGIT 63
           ++R++QT  + RMLN N P    STG  E V+K+LI+DRF Q+I+SPL+ VK+LR  GIT
Sbjct: 11  SIRERQTVALKRMLNFNVPHVKNSTG--EPVWKVLIYDRFGQDIISPLLSVKELRDMGIT 70

Query: 64  LYFLIDKDRKPVHDVPAVYFVQPSKINIDRIVADASRFLYDSFYLNFSSSIPRPLLEDLA 123
           L+ L+  DR P+ DVPAVYFV P++ NIDR+  D    LY+S+YLNF S+I R  LED+A
Sbjct: 71  LHLLLHSDRDPIPDVPAVYFVMPTEENIDRLCQDLRNQLYESYYLNFISAISRSKLEDIA 130

Query: 124 SGSLNSDSVQRIAKVHDQYLEFVTLEDNLFSLAQKS----IYVQLNDPSAGDREIEEIIE 183
           + +L + +V ++AKV DQYL F+TLED++F L  ++     Y  +N P   D E+E +++
Sbjct: 131 NAALAASAVTQVAKVFDQYLNFITLEDDMFVLCNQNKELVSYRAINRPDITDTEMETVMD 190

Query: 184 RIVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHFL-SKNNLFTEG--GGFAS 243
            IV  LF    TL  VP+IRC RG  AEMVA  LD++LR++   ++N+LFT    G    
Sbjct: 191 TIVDSLFCFFVTLGAVPIIRCSRGTAAEMVAVKLDKKLRENLRDARNSLFTGDPLGTGQF 250

Query: 244 SFQRPILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLSVQGEKG------------ 303
           SFQRP+L L DRN +L+  + H + Y+ LVHDVL   LNR++++   G            
Sbjct: 251 SFQRPLLVLVDRNIDLATPLHHTWTYQALVHDVLDFHLNRVNLEESTGVENSPAGARPKR 310

Query: 304 -GMKSYELDSSDPFWVANGSLEFPEVAVEIETQLNKYKRDVDEVNRRTG--GTDGAEFDG 363
              KSY+L   D FW  +    FPEVA  ++ +L  Y+   DEV R     G +G +   
Sbjct: 311 KNKKSYDLTPVDKFWQKHKGSPFPEVAESVQQELESYRAQEDEVKRLKSIMGLEGEDEGA 370

Query: 364 TDLIG-NTKHLMNAVNSLPELTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMAR 423
             ++  NT  L +AV+SLPEL E+K++ID HTN+AT +L  IK R LD Y + E  +M++
Sbjct: 371 ISMLSDNTAKLTSAVSSLPELLEKKRLIDLHTNVATAVLEHIKARKLDVYFEYEEKIMSK 430

Query: 424 GGIDRNELLSVLK--GKGTKTDKLRFAVIYLISSETI-NQSEVEAVETALRESEVDTSAF 483
             +D++ LL V+     GT  DK+R  +IY IS++   ++ ++E  + AL ++  + S  
Sbjct: 431 TTLDKS-LLDVISDPDAGTPEDKMRLFLIYYISAQQAPSEVDLEQYKKALTDAGCNLSPL 490

Query: 484 QYVKKIKSLNVSFSSANSASRSNI--VDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRT 543
           QY+K+ K+     S+  S   +    +    ++       V  GVKNL+   + L +TR 
Sbjct: 491 QYIKQWKAFAKMASTPASYGNTTTKPMGLLSRVMNTGSQFVMEGVKNLVLKQQNLPVTRI 550

Query: 544 VEGLMEGKLNPEIDTFLTFDPRAPKSSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQE 603
           ++ LME K NPE D +  FDP+  +S+  +S    K PF+EAIVF++GGGNY+EY +L +
Sbjct: 551 LDNLMEMKSNPETDDYRYFDPKMLRSND-SSVPRNKSPFQEAIVFVVGGGNYIEYQNLVD 610

Query: 604 LAMNQQPVKHIIYGSTEILTGVEFVEQLLLLGQK 610
               +Q  KHI+YG +EI    +F++QL  LGQK
Sbjct: 611 YIKAKQG-KHILYGCSEIFNATQFIKQLSQLGQK 639

BLAST of Lsi10G008400 vs. TrEMBL
Match: A0A0A0KSS4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G265810 PE=3 SV=1)

HSP 1 Score: 1182.9 bits (3059), Expect = 0.0e+00
Identity = 601/624 (96.31%), Postives = 613/624 (98.24%), Query Frame = 1

Query: 1   MALNLRQKQTECIIRMLNLNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKH 60
           MALNLRQKQTECIIRMLNLNQPVNSTS GNEEEVYKILIFDRFCQNILSPLIHVKDLRKH
Sbjct: 1   MALNLRQKQTECIIRMLNLNQPVNSTSAGNEEEVYKILIFDRFCQNILSPLIHVKDLRKH 60

Query: 61  GITLYFLIDKDRKPVHDVPAVYFVQPSKINIDRIVADASRFLYDSFYLNFSSSIPRPLLE 120
           GITLYFLIDKDRKPVHDVPAVYFVQP+KINIDRIVADASR LYDSFYLNFSSSIPRPLLE
Sbjct: 61  GITLYFLIDKDRKPVHDVPAVYFVQPTKINIDRIVADASRLLYDSFYLNFSSSIPRPLLE 120

Query: 121 DLASGSLNSDSVQRIAKVHDQYLEFVTLEDNLFSLAQKSIYVQLNDPSAGDREIEEIIER 180
           DLASG+LNSDSVQRI+KVHDQYLEFVTLEDNLFSLAQKSIY+QLNDPSAGDREIEEIIER
Sbjct: 121 DLASGTLNSDSVQRISKVHDQYLEFVTLEDNLFSLAQKSIYLQLNDPSAGDREIEEIIER 180

Query: 181 IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHFLSKNNLFTEGGGFASSFQR 240
           IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDH LSKNNLFTEGGGFASSFQR
Sbjct: 181 IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHLLSKNNLFTEGGGFASSFQR 240

Query: 241 PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLSVQGEKGGMKSYELDSSDPFWVA 300
           PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRL+VQGEKGGMKSYELDSSDPFWV 
Sbjct: 241 PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLNVQGEKGGMKSYELDSSDPFWVG 300

Query: 301 NGSLEFPEVAVEIETQLNKYKRDVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPE 360
           NGSLEFPEVAVEIETQLNKYK+DVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPE
Sbjct: 301 NGSLEFPEVAVEIETQLNKYKKDVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPE 360

Query: 361 LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGKGTKTD 420
           LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGKGTKTD
Sbjct: 361 LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGKGTKTD 420

Query: 421 KLRFAVIYLISSETINQSEVEAVETALRESEVDTSAFQYVKKIKSLNVSFSSANSASRSN 480
           KLRFAVIYLISSET+N SEVEAVE ALRESEVDTSAFQYVKKIKSLN SFSSANSASRSN
Sbjct: 421 KLRFAVIYLISSETLNPSEVEAVEAALRESEVDTSAFQYVKKIKSLNASFSSANSASRSN 480

Query: 481 IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMEGKLNPEIDTFLTFDPRAPK 540
           +VDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLM+G+ NPEIDTFLTFDPRAPK
Sbjct: 481 LVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMDGRPNPEIDTFLTFDPRAPK 540

Query: 541 SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELAMNQQPVKHIIYGSTEILTGVEFV 600
           SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQEL+MNQQP+KHIIYGSTEILTGVEFV
Sbjct: 541 SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELSMNQQPIKHIIYGSTEILTGVEFV 600

Query: 601 EQLLLLGQKMGLGNVAAAPPPPGR 625
           EQL LLGQKMG GNVAA PPPPGR
Sbjct: 601 EQLSLLGQKMGFGNVAAPPPPPGR 624

BLAST of Lsi10G008400 vs. TrEMBL
Match: B9MYY5_POPTR (SEC1-family transport family protein OS=Populus trichocarpa GN=POPTR_0004s21450g PE=3 SV=1)

HSP 1 Score: 1102.0 bits (2849), Expect = 0.0e+00
Identity = 552/621 (88.89%), Postives = 590/621 (95.01%), Query Frame = 1

Query: 1   MALNLRQKQTECIIRMLNLNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKH 60
           MALNLRQKQTECIIRMLNLNQP+N+T T NEE VYKILI+D+FCQNILSPLIHVKDLRKH
Sbjct: 1   MALNLRQKQTECIIRMLNLNQPLNATGTTNEE-VYKILIYDKFCQNILSPLIHVKDLRKH 60

Query: 61  GITLYFLIDKDRKPVHDVPAVYFVQPSKINIDRIVADASRFLYDSFYLNFSSSIPRPLLE 120
           G+TLYFLIDKDRKPVHDVPAVYFVQPSK+N+ RIVADAS+ LYDSF+LNFSSSIPRPLLE
Sbjct: 61  GVTLYFLIDKDRKPVHDVPAVYFVQPSKVNVQRIVADASQSLYDSFHLNFSSSIPRPLLE 120

Query: 121 DLASGSLNSDSVQRIAKVHDQYLEFVTLEDNLFSLAQKSIYVQLNDPSAGDREIEEIIER 180
           DLASG+LNS+S+ +I+KVHDQYLEFVTLE+NLFSLAQKS YVQLNDPSAGDREIEEI+E+
Sbjct: 121 DLASGTLNSESIDKISKVHDQYLEFVTLENNLFSLAQKSCYVQLNDPSAGDREIEEIVEK 180

Query: 181 IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHFLSKNNLFTEGGGFASSFQR 240
           + SGLFSVLATLAVVPVIRC RGGPAEMVAS LDQ+LRDH LSKNNLFTEGGGFASSFQR
Sbjct: 181 VASGLFSVLATLAVVPVIRCPRGGPAEMVASVLDQKLRDHLLSKNNLFTEGGGFASSFQR 240

Query: 241 PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLSVQGEKGGMKSYELDSSDPFWVA 300
           P+LCLFDRNFELSVGIQHDFRYRPLVHDVLGL+LNRLSVQGEKGGM+SYELDSSDPFWVA
Sbjct: 241 PVLCLFDRNFELSVGIQHDFRYRPLVHDVLGLRLNRLSVQGEKGGMRSYELDSSDPFWVA 300

Query: 301 NGSLEFPEVAVEIETQLNKYKRDVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPE 360
           NGSLEFPEVAVEIETQLNKYK+DVDEVNRRTG TDGAEFDGTDLIGNTKHLMNAVNSLPE
Sbjct: 301 NGSLEFPEVAVEIETQLNKYKKDVDEVNRRTGETDGAEFDGTDLIGNTKHLMNAVNSLPE 360

Query: 361 LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGKGTKTD 420
           LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMM RGGIDRNELL VL+GKGTK D
Sbjct: 361 LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMVRGGIDRNELLGVLRGKGTKID 420

Query: 421 KLRFAVIYLISSETINQSEVEAVETALRESEVDTSAFQYVKKIKSLNVSFSSANSASRSN 480
           KLRFA+IYLI SE+INQSEVEA+ETALRESEVD  AFQYVKK+KSLNVS +SANSASRSN
Sbjct: 421 KLRFAIIYLICSESINQSEVEAIETALRESEVDNCAFQYVKKMKSLNVSLASANSASRSN 480

Query: 481 IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMEGKLNPEIDTFLTFDPRAPK 540
           IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVE LMEGK NPE+D++L FDPRAPK
Sbjct: 481 IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEALMEGKPNPEVDSYLVFDPRAPK 540

Query: 541 SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELAMNQQPVKHIIYGSTEILTGVEFV 600
           S SGTSSSHLKGPFKEAIVFMIGGGNY+EYGSLQE A  QQPVKH+IYG+TEILTG+EFV
Sbjct: 541 SGSGTSSSHLKGPFKEAIVFMIGGGNYMEYGSLQEFAQRQQPVKHVIYGTTEILTGMEFV 600

Query: 601 EQLLLLGQKMGLGNVAAAPPP 622
           EQL +LGQKMGLG+  A P P
Sbjct: 601 EQLTVLGQKMGLGSSVAPPAP 620

BLAST of Lsi10G008400 vs. TrEMBL
Match: A0A061E5A4_THECC (Sec1/munc18-like (SM) proteins superfamily OS=Theobroma cacao GN=TCM_006465 PE=3 SV=1)

HSP 1 Score: 1086.6 bits (2809), Expect = 0.0e+00
Identity = 547/617 (88.65%), Postives = 581/617 (94.17%), Query Frame = 1

Query: 1   MALNLRQKQTECIIRMLNLNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKH 60
           MALNLRQKQTECIIRMLNLNQPVN + T NEE VYKILI+DRFCQNILSPLIHVKDLRKH
Sbjct: 1   MALNLRQKQTECIIRMLNLNQPVNPSGTANEE-VYKILIYDRFCQNILSPLIHVKDLRKH 60

Query: 61  GITLYFLIDKDRKPVHDVPAVYFVQPSKINIDRIVADASRFLYDSFYLNFSSSIPRPLLE 120
           G+TLYFLIDKDRKPVHDVPAVYFVQPS  NI RIVADASR LYDSF+LNFSSSIPRPLLE
Sbjct: 61  GVTLYFLIDKDRKPVHDVPAVYFVQPSHSNIQRIVADASRSLYDSFHLNFSSSIPRPLLE 120

Query: 121 DLASGSLNSDSVQRIAKVHDQYLEFVTLEDNLFSLAQKSIYVQLNDPSAGDREIEEIIER 180
           DLASG+LNSDS+ RI+KVHDQYLEFVTLEDNLFSL+QKS YVQLNDPSAGD+EIE+IIER
Sbjct: 121 DLASGTLNSDSIHRISKVHDQYLEFVTLEDNLFSLSQKSTYVQLNDPSAGDKEIEDIIER 180

Query: 181 IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHFLSKNNLFTEGGGFASSFQR 240
           +VSGLF VLATL+VVP+IRC RGGPAE+VASALDQ+LRDH LSKNNLF+EGG F SSFQR
Sbjct: 181 VVSGLFCVLATLSVVPIIRCPRGGPAELVASALDQKLRDHLLSKNNLFSEGGSFVSSFQR 240

Query: 241 PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLSVQGEKGGMKSYELDSSDPFWVA 300
           PILC+FDRNFELS G+QHDFRYRPLVHD+LGLKLNRLSV GEKGGMKSYELDSSDPFW+A
Sbjct: 241 PILCVFDRNFELSAGLQHDFRYRPLVHDILGLKLNRLSVPGEKGGMKSYELDSSDPFWMA 300

Query: 301 NGSLEFPEVAVEIETQLNKYKRDVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPE 360
           NGSLEFPEVAVEIETQLNKYK+DVDEVNRRTGGT G EFDGTDLIGNTKHLMNAVNSLPE
Sbjct: 301 NGSLEFPEVAVEIETQLNKYKKDVDEVNRRTGGTAGTEFDGTDLIGNTKHLMNAVNSLPE 360

Query: 361 LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGKGTKTD 420
           LT+RKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMM RG IDRNELLSVLKGKGTKTD
Sbjct: 361 LTDRKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMVRGSIDRNELLSVLKGKGTKTD 420

Query: 421 KLRFAVIYLISSETINQSEVEAVETALRESEVDTSAFQYVKKIKSLNVSFSSANSASRSN 480
           KLRFA++Y+ISSETIN SE EAVE ALRES+VDTSAFQYVKKIKSLNVS +SANSASR+N
Sbjct: 421 KLRFAIMYIISSETINPSEAEAVEAALRESDVDTSAFQYVKKIKSLNVSLASANSASRNN 480

Query: 481 IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMEGKLNPEIDTFLTFDPRAPK 540
           IVDWAEK+YGQSISAVTAGVKNLLSSDRQLALTR VE LMEGK NPEID++L FDPRAPK
Sbjct: 481 IVDWAEKIYGQSISAVTAGVKNLLSSDRQLALTRIVEALMEGKPNPEIDSYLVFDPRAPK 540

Query: 541 SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELAMNQQPVKHIIYGSTEILTGVEFV 600
           SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQEL   QQPVK++IYG+TEILTG EFV
Sbjct: 541 SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELVQRQQPVKYVIYGTTEILTGSEFV 600

Query: 601 EQLLLLGQKMGLGNVAA 618
           EQL LLGQKMGLGN  A
Sbjct: 601 EQLALLGQKMGLGNTVA 616

BLAST of Lsi10G008400 vs. TrEMBL
Match: A0A0D2TWT9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G040800 PE=3 SV=1)

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 544/618 (88.03%), Postives = 583/618 (94.34%), Query Frame = 1

Query: 1   MALNLRQKQTECIIRMLNLNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKH 60
           MALNLRQKQTECIIRMLNLNQPVN + T NEE VYKILI+D+FCQNILSPLIHVKDLRKH
Sbjct: 1   MALNLRQKQTECIIRMLNLNQPVNPSGTANEE-VYKILIYDKFCQNILSPLIHVKDLRKH 60

Query: 61  GITLYFLIDKDRKPVHDVPAVYFVQPSKINIDRIVADASRFLYDSFYLNFSSSIPRPLLE 120
           G+TLYFLIDKDRKPVHDVPAVYFVQP+  NI RIVADASR LYDSF+LNFSSSIPRPLLE
Sbjct: 61  GVTLYFLIDKDRKPVHDVPAVYFVQPNNPNIQRIVADASRSLYDSFHLNFSSSIPRPLLE 120

Query: 121 DLASGSLNSDSVQRIAKVHDQYLEFVTLEDNLFSLAQKSIYVQLNDPSAGDREIEEIIER 180
           DLASG+LNSDS+ RI+KVHDQYLEFVTLEDNLFSL+QKS YVQLNDPSAGD+EIE+IIER
Sbjct: 121 DLASGTLNSDSIHRISKVHDQYLEFVTLEDNLFSLSQKSTYVQLNDPSAGDKEIEDIIER 180

Query: 181 IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHFLSKNNLFTEGGGFASSFQR 240
           +V+GLF VLATLA VP+IRC RGGPAEMVASALDQ+LRDH LSKNNLF+EGGGF SSFQR
Sbjct: 181 VVNGLFCVLATLAAVPIIRCPRGGPAEMVASALDQKLRDHLLSKNNLFSEGGGFVSSFQR 240

Query: 241 PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLSVQGEKGGMKSYELDSSDPFWVA 300
           PILC+FDRNFELS GIQHDFRYRPLVHD+LGLKLNRLSV GEKGGMKS+ELDSSDPFW+A
Sbjct: 241 PILCIFDRNFELSAGIQHDFRYRPLVHDILGLKLNRLSVPGEKGGMKSFELDSSDPFWMA 300

Query: 301 NGSLEFPEVAVEIETQLNKYKRDVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPE 360
           NGSLEFPEVAVEIETQLNKYK+DVDEVN+RTGGT GAEFDGTDLIGNTKHLMNAVNSLPE
Sbjct: 301 NGSLEFPEVAVEIETQLNKYKKDVDEVNKRTGGTAGAEFDGTDLIGNTKHLMNAVNSLPE 360

Query: 361 LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGKGTKTD 420
           LTERKQVIDKHTNIATVLLGEIKERSLD+Y KKENDMM RGGIDRNEL+SVLKGKGTK D
Sbjct: 361 LTERKQVIDKHTNIATVLLGEIKERSLDAYVKKENDMMVRGGIDRNELMSVLKGKGTKID 420

Query: 421 KLRFAVIYLISSETINQSEVEAVETALRESEVDTSAFQYVKKIKSLNVSFSSANSASRSN 480
           KLRFA++Y+ISSETIN SE EAVE ALRESEVDTSAFQYVKKIKSLNVS +SANSASRSN
Sbjct: 421 KLRFAIMYIISSETINPSEAEAVEAALRESEVDTSAFQYVKKIKSLNVSLASANSASRSN 480

Query: 481 IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMEGKLNPEIDTFLTFDPRAPK 540
           IVDWAEKLYGQSISAVTAG+KNLLS DRQLALTRTVE LMEGK NPEID++L FDPRAPK
Sbjct: 481 IVDWAEKLYGQSISAVTAGMKNLLSGDRQLALTRTVEALMEGKPNPEIDSYLVFDPRAPK 540

Query: 541 SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELAMNQQPVKHIIYGSTEILTGVEFV 600
           SS GTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQ+LA +QQP K +IYG+TEILTG+EFV
Sbjct: 541 SSPGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQDLAQHQQPAKQVIYGTTEILTGMEFV 600

Query: 601 EQLLLLGQKMGLGNVAAA 619
           EQL LLGQKMGLG+ +AA
Sbjct: 601 EQLSLLGQKMGLGSSSAA 617

BLAST of Lsi10G008400 vs. TrEMBL
Match: A0A0B0NR49_GOSAR (SEC1 family transport protein SLY1 OS=Gossypium arboreum GN=F383_06667 PE=3 SV=1)

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 544/618 (88.03%), Postives = 584/618 (94.50%), Query Frame = 1

Query: 1   MALNLRQKQTECIIRMLNLNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKH 60
           MALNLRQKQTECIIRMLNLNQPVN + T NEE VYKILI+D+FCQNILSPLIHVKDLRKH
Sbjct: 1   MALNLRQKQTECIIRMLNLNQPVNPSGTANEE-VYKILIYDKFCQNILSPLIHVKDLRKH 60

Query: 61  GITLYFLIDKDRKPVHDVPAVYFVQPSKINIDRIVADASRFLYDSFYLNFSSSIPRPLLE 120
           G+TLYFLIDKDRKPVHDVPAVYFVQP+  NI RIVADASR LYDSF+LNFSSSIPRPLLE
Sbjct: 61  GVTLYFLIDKDRKPVHDVPAVYFVQPNNPNIQRIVADASRSLYDSFHLNFSSSIPRPLLE 120

Query: 121 DLASGSLNSDSVQRIAKVHDQYLEFVTLEDNLFSLAQKSIYVQLNDPSAGDREIEEIIER 180
           DLASG+LNSD++ RI+KVHDQYLEFVTLEDNLFSL+QKS YVQLNDPSAGD+EIE+IIER
Sbjct: 121 DLASGTLNSDAIHRISKVHDQYLEFVTLEDNLFSLSQKSTYVQLNDPSAGDKEIEDIIER 180

Query: 181 IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHFLSKNNLFTEGGGFASSFQR 240
           +V+GLF VLATLAVVP+IRC RGGPAEMVASALDQ+LRDH LSKNNLF+EGGGF SSFQR
Sbjct: 181 VVNGLFCVLATLAVVPIIRCPRGGPAEMVASALDQKLRDHLLSKNNLFSEGGGFVSSFQR 240

Query: 241 PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLSVQGEKGGMKSYELDSSDPFWVA 300
           PILC+FDRNFELS GIQHDFRYRPLVHD+LGLKLNRLSV GEKGGMKS+ELDSSD FW+A
Sbjct: 241 PILCIFDRNFELSAGIQHDFRYRPLVHDILGLKLNRLSVPGEKGGMKSFELDSSDTFWMA 300

Query: 301 NGSLEFPEVAVEIETQLNKYKRDVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPE 360
           NGSLEFPEVAVEIETQLNKYK+DVDEVN+RTGGT GAEFDGTDLIGNTKHLMNAVNSLPE
Sbjct: 301 NGSLEFPEVAVEIETQLNKYKKDVDEVNKRTGGTAGAEFDGTDLIGNTKHLMNAVNSLPE 360

Query: 361 LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGKGTKTD 420
           LTERKQVIDKHTNIATVLLGEIKERSLD+Y KKENDMM RGGIDRNEL+SVLKGKGTK D
Sbjct: 361 LTERKQVIDKHTNIATVLLGEIKERSLDAYVKKENDMMVRGGIDRNELMSVLKGKGTKID 420

Query: 421 KLRFAVIYLISSETINQSEVEAVETALRESEVDTSAFQYVKKIKSLNVSFSSANSASRSN 480
           KLRFA++Y+ISSETIN SE EAVE ALRESEVDTSAFQYVKKIKSLNVS +SANSASRSN
Sbjct: 421 KLRFAIMYIISSETINPSEAEAVEAALRESEVDTSAFQYVKKIKSLNVSLASANSASRSN 480

Query: 481 IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMEGKLNPEIDTFLTFDPRAPK 540
           IVDWAEKLYGQSISAVTAG+KNLLS D+QLALTRTVE LMEGK NPEID++L FDPRAPK
Sbjct: 481 IVDWAEKLYGQSISAVTAGMKNLLSGDKQLALTRTVEALMEGKPNPEIDSYLVFDPRAPK 540

Query: 541 SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELAMNQQPVKHIIYGSTEILTGVEFV 600
           SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQ+LA +QQP K +IYG+TEILTG+EFV
Sbjct: 541 SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQDLAQHQQPAKQVIYGTTEILTGMEFV 600

Query: 601 EQLLLLGQKMGLGNVAAA 619
           EQL LLGQKMGLG+ AAA
Sbjct: 601 EQLSLLGQKMGLGSSAAA 617

BLAST of Lsi10G008400 vs. TAIR10
Match: AT2G17980.1 (AT2G17980.1 Sec1/munc18-like (SM) proteins superfamily)

HSP 1 Score: 978.4 bits (2528), Expect = 2.1e-285
Identity = 495/622 (79.58%), Postives = 555/622 (89.23%), Query Frame = 1

Query: 1   MALNLRQKQTECIIRMLNLNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKH 60
           MALNLRQKQTEC+IRMLNLNQP+N + T NEE VYKILI+DRFCQNILSPL HVKDLRKH
Sbjct: 1   MALNLRQKQTECVIRMLNLNQPLNPSGTANEE-VYKILIYDRFCQNILSPLTHVKDLRKH 60

Query: 61  GITLYFLIDKDRKPVHDVPAVYFVQPSKINIDRIVADASRFLYDSFYLNFSSSIPRPLLE 120
           G+TL+FLIDKDR+PVHDVPAVYFVQP++ N+ RI+ADASR LYD+F+LNFSSSIPR  LE
Sbjct: 61  GVTLFFLIDKDRQPVHDVPAVYFVQPTESNLQRIIADASRSLYDTFHLNFSSSIPRKFLE 120

Query: 121 DLASGSLNSDSVQRIAKVHDQYLEFVTLEDNLFSLAQKSIYVQLNDPSAGDREIEEIIER 180
           +LASG+L S SV++++KVHDQYLEFVTLEDNLFSLAQ+S YVQ+NDPSAG++EI EIIER
Sbjct: 121 ELASGTLKSGSVEKVSKVHDQYLEFVTLEDNLFSLAQQSTYVQMNDPSAGEKEINEIIER 180

Query: 181 IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHFLSKNNLFTEGGGFASSFQR 240
           + SGLF VL TL VVPVIRC  GGPAEMVAS LDQ+LRDH LSKNNLFTEGGGF SSFQR
Sbjct: 181 VASGLFCVLVTLGVVPVIRCPSGGPAEMVASLLDQKLRDHLLSKNNLFTEGGGFMSSFQR 240

Query: 241 PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLSVQGEKGGMKSYELDSSDPFWVA 300
           P+LC+FDRNFELSVGIQHDFRYRPLVHDVLGLKLN+L VQGEKG  KS+ELDSSDPFW A
Sbjct: 241 PLLCIFDRNFELSVGIQHDFRYRPLVHDVLGLKLNQLKVQGEKGPPKSFELDSSDPFWSA 300

Query: 301 NGSLEFPEVAVEIETQLNKYKRDVDEVNRRTGGTDGAEFDGTDLIGN--TKHLMNAVNSL 360
           N +LEFP+VAVEIETQLNKYKRDV+EVN++TGG  GAEFDGTDLIGN  T+HLMN V SL
Sbjct: 301 NSTLEFPDVAVEIETQLNKYKRDVEEVNKKTGGGSGAEFDGTDLIGNIHTEHLMNTVKSL 360

Query: 361 PELTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGKGTK 420
           PELTERK+VIDKHTNIAT LLG+IKERS+D++ KKE+DMM RGGIDR EL++ LKGKGTK
Sbjct: 361 PELTERKKVIDKHTNIATALLGQIKERSIDAFTKKESDMMMRGGIDRTELMAALKGKGTK 420

Query: 421 TDKLRFAVIYLISSETINQSEVEAVETALRESEVDTSAFQYVKKIKSLNVSF--SSANSA 480
            DKLRFA++YLIS+ETINQSEVEAVE AL E+E DTSAFQYVKKIKSLN SF  +SANSA
Sbjct: 421 MDKLRFAIMYLISTETINQSEVEAVEAALNEAEADTSAFQYVKKIKSLNASFAATSANSA 480

Query: 481 SRSNIVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMEGKLNPEIDTFLTFDP 540
           SRSNIVDWAEKLYGQSISAVTAGVKNLLSSD+QLA+TRTVE L EGK NPEID++   DP
Sbjct: 481 SRSNIVDWAEKLYGQSISAVTAGVKNLLSSDQQLAVTRTVEALTEGKPNPEIDSYRFLDP 540

Query: 541 RAPKSSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELAMNQQPVKHIIYGSTEILTG 600
           RAPKSSS +  SH+KGPF+EAIVFMIGGGNYVEYGSLQEL   Q  VK++IYG+TEIL G
Sbjct: 541 RAPKSSS-SGGSHVKGPFREAIVFMIGGGNYVEYGSLQELTQRQLTVKNVIYGATEILNG 600

Query: 601 VEFVEQLLLLGQKMGLGNVAAA 619
            E VEQL LLG+KMGLG   A+
Sbjct: 601 GELVEQLGLLGKKMGLGGPVAS 620

BLAST of Lsi10G008400 vs. TAIR10
Match: AT4G31740.1 (AT4G31740.1 Sec1/munc18-like (SM) proteins superfamily)

HSP 1 Score: 222.6 bits (566), Expect = 6.6e-58
Identity = 125/193 (64.77%), Postives = 143/193 (74.09%), Query Frame = 1

Query: 420 DKLRFAVIYLISSETINQSEVEAVETALRESEVDTSAFQYVKKIKSLNVSFSSANSASRS 479
           DKLRFA++YL+S ETINQSEVEAVE AL                        SA+SASRS
Sbjct: 2   DKLRFAIMYLLSLETINQSEVEAVEAAL-----------------------PSADSASRS 61

Query: 480 NIVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMEGKLNPEIDTFLTFDPRAP 539
           NIVDWAEKLYGQSISAVT GVKNLLSSD+QL + RTVE L +GK NPE D++L  D RA 
Sbjct: 62  NIVDWAEKLYGQSISAVTPGVKNLLSSDQQLPVARTVEALTDGKPNPETDSYLILDARAS 121

Query: 540 KSSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELAMNQQPVKHIIYGSTEILTGVEF 599
           KS S   +S++KGPF+EAIVFMIGGGNY+EY SLQEL+  Q+ V +IIYG+TEILTG E 
Sbjct: 122 KSGS-IDNSYVKGPFEEAIVFMIGGGNYIEYSSLQELSQRQEMVNNIIYGATEILTGTEL 170

Query: 600 VEQLLLLGQKMGL 613
           VEQL  LGQKMGL
Sbjct: 182 VEQLGELGQKMGL 170

BLAST of Lsi10G008400 vs. TAIR10
Match: AT4G36100.1 (AT4G36100.1 Sec1/munc18-like (SM) proteins superfamily)

HSP 1 Score: 145.2 bits (365), Expect = 1.3e-34
Identity = 85/137 (62.04%), Postives = 104/137 (75.91%), Query Frame = 1

Query: 382 IKERSLDSYAKKENDMMAR-GGIDRNELLSVLKGKGTKTDKLRFAVIYLISSETINQSEV 441
           + E+SLD+Y +KE +MM   G I+R ELLSVLK KGT  DKLRFA++YLIS E++NQ+EV
Sbjct: 105 LMEQSLDAYDEKEKEMMMMIGSINRTELLSVLKAKGTNIDKLRFAIMYLISLESVNQTEV 164

Query: 442 EAVETALRESEVDTSAFQYVKKIKSLNVSFSSANSASRSNIVDWAEKLYGQSISAVTAGV 501
           EAVE ALRE+++DTS FQYVKKIKSLNVS  +ANSAS+S+I  W     G       AGV
Sbjct: 165 EAVEAALREAKIDTSTFQYVKKIKSLNVSL-AANSASKSHIALWPVYKRGD-----RAGV 224

Query: 502 KNLLSSDRQLALTRTVE 518
           KNLLSSD +LA+ R VE
Sbjct: 225 KNLLSSDEKLAVARAVE 235

BLAST of Lsi10G008400 vs. TAIR10
Match: AT1G12360.1 (AT1G12360.1 Sec1/munc18-like (SM) proteins superfamily)

HSP 1 Score: 112.5 bits (280), Expect = 9.7e-25
Identity = 99/434 (22.81%), Postives = 194/434 (44.70%), Query Frame = 1

Query: 13  IIRMLNLNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKHGITLYFLIDKDR 72
           I R   L + + S  TG+ +  +K+LI D+    I+S    + D+ + G++L   I + R
Sbjct: 21  ITRERLLYEMLRSAKTGSSKSTWKVLIMDKLTVKIMSYACKMADITQEGVSLVEDIFRRR 80

Query: 73  KPVHDVPAVYFVQPSKINIDRIVADAS--RFLYDSFYLNFSSSIPRPLLEDLASGSLNSD 132
           +P+  + A+YF+QP+K N+   ++D S    LY   ++ FSS + + L+     G +  D
Sbjct: 81  QPLPSMDAIYFIQPTKENVIMFLSDMSGKSPLYKKAFVFFSSPVSKELV-----GHIKKD 140

Query: 133 S--VQRIAKVHDQYLEFVTLEDNLFSLAQKSIYVQLNDPSAGDREIEEIIERIVSGLFSV 192
           S  + RI  + +  LEF  ++   F    +     L       R+ +  +  + S + +V
Sbjct: 141 SSVLPRIGALREMNLEFFAIDSQGFITDHERALEDLFGDEETSRKGDACLNVMASRIATV 200

Query: 193 LATLAVVPVIRCQRGGPAEMVASALDQRLRDHFLSKN-----NLFTEGGGFASSFQRPIL 252
            A+L   P +R +    A+ + ++    LRD   +K      N   +      +F +   
Sbjct: 201 FASLREFPAVRYR---AAKSLDASTMTTLRDLIPTKLAAGIWNCLAKHKQSIENFPQTET 260

Query: 253 C---LFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRL-----SVQGEKGGMKSYELDSSD 312
           C   + DR+ +    + H++ Y  + HD+L ++ N+      S  G +   K   L+  D
Sbjct: 261 CELLILDRSIDQIAPVIHEWTYDAMCHDLLNMEGNKYVHVIPSKSGGQPEKKDVLLEEHD 320

Query: 313 PFWVANGSLEFPEVAVEIETQLNKYKRDVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAV 372
           P W+        + +  +  ++  +    ++  +  G  DGAE        +T+ L   V
Sbjct: 321 PIWLELRHAHIADASERLHDKMTNF-LSKNKAAQLQGKRDGAEL-------STRDLQKMV 380

Query: 373 NSLPELTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVL--K 428
            +LP+ +E+   +  H  IA  L   I+E+ L    + E D++  G     +++  L  +
Sbjct: 381 QALPQYSEQIDKLSLHVEIARKLNDLIREQGLRELGQLEQDLVF-GDAGMKDVIKYLSTQ 437

BLAST of Lsi10G008400 vs. TAIR10
Match: AT1G02010.1 (AT1G02010.1 secretory 1A)

HSP 1 Score: 109.4 bits (272), Expect = 8.2e-24
Identity = 98/426 (23.00%), Postives = 197/426 (46.24%), Query Frame = 1

Query: 19  LNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKHGITLYFLIDKDRKPVHDV 78
           L++ + ST TG + + +KILI DR    ++S    + D+   GI+L   + K R+P+  +
Sbjct: 30  LHEMLGSTKTG-DSKAWKILIMDRVTVKVMSQSCKMADITDQGISLVEELFKRREPMPGM 89

Query: 79  PAVYFVQPSKINIDRIVADAS--RFLYDSFYLNFSSSIPRPLLEDLASGSLNSDSVQRIA 138
            A+YF+QPSK NI   ++D S    LY   ++ FSS+IP+ L+  + S   +S  + RI 
Sbjct: 90  DAIYFIQPSKENIVMFLSDMSGREPLYRKAFIFFSSTIPKELVNHIKS---DSSVLPRIG 149

Query: 139 KVHDQYLEFVTLEDNLF-SLAQKSIYVQLNDPSAGDREIEEIIERIVSGLFSVLATLAVV 198
            + +  +E+  +++  F +  ++++     + +   R     +  + + + +V A+L  +
Sbjct: 150 ALREMNMEYFPIDNQGFLTDHEQALETLYAEDAENSRHFHICLNIMATRIATVFASLKEL 209

Query: 199 PVIR---CQRGGPAEMVASALDQRLRDHFLSKNNLFTEGGGFASSFQRPILC---LFDRN 258
           P +R    +     ++V S L   + D  +SK            +F +   C   + DR+
Sbjct: 210 PFVRYRAAKSTASRDLVPSKLAAAIWD-CISKYKAI-------PNFPQTETCELLIVDRS 269

Query: 259 FELSVGIQHDFRYRPLVHDVLGLKLNR--LSVQGEKGG---MKSYELDSSDPFWVANGSL 318
            +    I H++ Y  + HD+L ++ N+  + V  + GG    K   L+  DP W+     
Sbjct: 270 VDQIAPIIHEWTYDAMCHDLLDMEGNKHVIEVPSKTGGPPEKKEIVLEDHDPVWLELRHT 329

Query: 319 EFPEVAVEIETQLNKYKRDVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPELTER 378
              + +  +  ++  +         R+        DG++L  +T+ L   V +LP+  E+
Sbjct: 330 HIADASERLHEKMTNFASKNKAAQMRSR-------DGSEL--STRDLQKIVQALPQYGEQ 389

Query: 379 KQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGK--GTKTDKL 429
              +  H  +A  +   I++  L    + E D++  G     ++++ L+        +KL
Sbjct: 390 VDKLSTHVELAGKINRIIRDTGLRDLGQLEQDLVF-GDAGAKDVINFLRTNQDTNPENKL 433

BLAST of Lsi10G008400 vs. NCBI nr
Match: gi|449445286|ref|XP_004140404.1| (PREDICTED: SEC1 family transport protein SLY1-like [Cucumis sativus])

HSP 1 Score: 1182.9 bits (3059), Expect = 0.0e+00
Identity = 601/624 (96.31%), Postives = 613/624 (98.24%), Query Frame = 1

Query: 1   MALNLRQKQTECIIRMLNLNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKH 60
           MALNLRQKQTECIIRMLNLNQPVNSTS GNEEEVYKILIFDRFCQNILSPLIHVKDLRKH
Sbjct: 1   MALNLRQKQTECIIRMLNLNQPVNSTSAGNEEEVYKILIFDRFCQNILSPLIHVKDLRKH 60

Query: 61  GITLYFLIDKDRKPVHDVPAVYFVQPSKINIDRIVADASRFLYDSFYLNFSSSIPRPLLE 120
           GITLYFLIDKDRKPVHDVPAVYFVQP+KINIDRIVADASR LYDSFYLNFSSSIPRPLLE
Sbjct: 61  GITLYFLIDKDRKPVHDVPAVYFVQPTKINIDRIVADASRLLYDSFYLNFSSSIPRPLLE 120

Query: 121 DLASGSLNSDSVQRIAKVHDQYLEFVTLEDNLFSLAQKSIYVQLNDPSAGDREIEEIIER 180
           DLASG+LNSDSVQRI+KVHDQYLEFVTLEDNLFSLAQKSIY+QLNDPSAGDREIEEIIER
Sbjct: 121 DLASGTLNSDSVQRISKVHDQYLEFVTLEDNLFSLAQKSIYLQLNDPSAGDREIEEIIER 180

Query: 181 IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHFLSKNNLFTEGGGFASSFQR 240
           IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDH LSKNNLFTEGGGFASSFQR
Sbjct: 181 IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHLLSKNNLFTEGGGFASSFQR 240

Query: 241 PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLSVQGEKGGMKSYELDSSDPFWVA 300
           PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRL+VQGEKGGMKSYELDSSDPFWV 
Sbjct: 241 PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLNVQGEKGGMKSYELDSSDPFWVG 300

Query: 301 NGSLEFPEVAVEIETQLNKYKRDVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPE 360
           NGSLEFPEVAVEIETQLNKYK+DVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPE
Sbjct: 301 NGSLEFPEVAVEIETQLNKYKKDVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPE 360

Query: 361 LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGKGTKTD 420
           LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGKGTKTD
Sbjct: 361 LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGKGTKTD 420

Query: 421 KLRFAVIYLISSETINQSEVEAVETALRESEVDTSAFQYVKKIKSLNVSFSSANSASRSN 480
           KLRFAVIYLISSET+N SEVEAVE ALRESEVDTSAFQYVKKIKSLN SFSSANSASRSN
Sbjct: 421 KLRFAVIYLISSETLNPSEVEAVEAALRESEVDTSAFQYVKKIKSLNASFSSANSASRSN 480

Query: 481 IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMEGKLNPEIDTFLTFDPRAPK 540
           +VDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLM+G+ NPEIDTFLTFDPRAPK
Sbjct: 481 LVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMDGRPNPEIDTFLTFDPRAPK 540

Query: 541 SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELAMNQQPVKHIIYGSTEILTGVEFV 600
           SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQEL+MNQQP+KHIIYGSTEILTGVEFV
Sbjct: 541 SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELSMNQQPIKHIIYGSTEILTGVEFV 600

Query: 601 EQLLLLGQKMGLGNVAAAPPPPGR 625
           EQL LLGQKMG GNVAA PPPPGR
Sbjct: 601 EQLSLLGQKMGFGNVAAPPPPPGR 624

BLAST of Lsi10G008400 vs. NCBI nr
Match: gi|659120511|ref|XP_008460227.1| (PREDICTED: SEC1 family transport protein SLY1 [Cucumis melo])

HSP 1 Score: 1181.4 bits (3055), Expect = 0.0e+00
Identity = 600/624 (96.15%), Postives = 613/624 (98.24%), Query Frame = 1

Query: 1   MALNLRQKQTECIIRMLNLNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKH 60
           MALNLRQKQTECIIRMLNLNQPVNSTS GNEEEVYKILIFDRFCQNILSPLIHVKDLRKH
Sbjct: 1   MALNLRQKQTECIIRMLNLNQPVNSTSAGNEEEVYKILIFDRFCQNILSPLIHVKDLRKH 60

Query: 61  GITLYFLIDKDRKPVHDVPAVYFVQPSKINIDRIVADASRFLYDSFYLNFSSSIPRPLLE 120
           GITLYFLIDKDRKPVHDVPAVYFVQP+KINIDRIVADASRFLYDSFY+NFSSSIPRPLLE
Sbjct: 61  GITLYFLIDKDRKPVHDVPAVYFVQPTKINIDRIVADASRFLYDSFYINFSSSIPRPLLE 120

Query: 121 DLASGSLNSDSVQRIAKVHDQYLEFVTLEDNLFSLAQKSIYVQLNDPSAGDREIEEIIER 180
           DLASG+LNSDSVQRI+KVHDQYLEFVTLEDNLFSLAQKSIY+QLNDPSAGDREIEEIIER
Sbjct: 121 DLASGTLNSDSVQRISKVHDQYLEFVTLEDNLFSLAQKSIYLQLNDPSAGDREIEEIIER 180

Query: 181 IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHFLSKNNLFTEGGGFASSFQR 240
           IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDH LSKNNLFTEGGGFASSFQR
Sbjct: 181 IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHLLSKNNLFTEGGGFASSFQR 240

Query: 241 PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLSVQGEKGGMKSYELDSSDPFWVA 300
           PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRL+VQGEKGGMKSYELDSSDPFWVA
Sbjct: 241 PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLNVQGEKGGMKSYELDSSDPFWVA 300

Query: 301 NGSLEFPEVAVEIETQLNKYKRDVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPE 360
           NGSLEFPEVAVEIETQLNKYK+DVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPE
Sbjct: 301 NGSLEFPEVAVEIETQLNKYKKDVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPE 360

Query: 361 LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGKGTKTD 420
           LTERKQVIDKHTNIATVLLG IK+RSLDSYAKKENDMM RGGIDRNELLSVLKGKGTKTD
Sbjct: 361 LTERKQVIDKHTNIATVLLGAIKDRSLDSYAKKENDMMVRGGIDRNELLSVLKGKGTKTD 420

Query: 421 KLRFAVIYLISSETINQSEVEAVETALRESEVDTSAFQYVKKIKSLNVSFSSANSASRSN 480
           KLRFAVIYLISSET+N SEVEAVE ALRESEVDTSAFQYVKKIKSLN SFSSANSASRSN
Sbjct: 421 KLRFAVIYLISSETLNPSEVEAVEAALRESEVDTSAFQYVKKIKSLNASFSSANSASRSN 480

Query: 481 IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMEGKLNPEIDTFLTFDPRAPK 540
           +VDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLM+G+ NPEIDTFLTFDPRAPK
Sbjct: 481 LVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMDGRPNPEIDTFLTFDPRAPK 540

Query: 541 SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELAMNQQPVKHIIYGSTEILTGVEFV 600
           SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQEL+MNQQPVKHIIYGSTEILTGVEFV
Sbjct: 541 SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELSMNQQPVKHIIYGSTEILTGVEFV 600

Query: 601 EQLLLLGQKMGLGNVAAAPPPPGR 625
           EQL LLGQKMG GNVAA PPPPGR
Sbjct: 601 EQLSLLGQKMGFGNVAAPPPPPGR 624

BLAST of Lsi10G008400 vs. NCBI nr
Match: gi|743895028|ref|XP_011040777.1| (PREDICTED: SEC1 family transport protein SLY1-like [Populus euphratica])

HSP 1 Score: 1102.4 bits (2850), Expect = 0.0e+00
Identity = 552/624 (88.46%), Postives = 594/624 (95.19%), Query Frame = 1

Query: 1   MALNLRQKQTECIIRMLNLNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKH 60
           MALNLRQKQTECIIRMLNLNQP+N+T T NEE VYKILI+D+FCQNILSPLIHVKDLRKH
Sbjct: 1   MALNLRQKQTECIIRMLNLNQPLNATGTTNEE-VYKILIYDKFCQNILSPLIHVKDLRKH 60

Query: 61  GITLYFLIDKDRKPVHDVPAVYFVQPSKINIDRIVADASRFLYDSFYLNFSSSIPRPLLE 120
           G+TLYFLIDKDRKPVHDVPAVYFVQPS++N+ RIVADAS+ LYDSF+LNFSSSIPRPLLE
Sbjct: 61  GVTLYFLIDKDRKPVHDVPAVYFVQPSQVNVQRIVADASQSLYDSFHLNFSSSIPRPLLE 120

Query: 121 DLASGSLNSDSVQRIAKVHDQYLEFVTLEDNLFSLAQKSIYVQLNDPSAGDREIEEIIER 180
           DLASG+LNS+S+++I+KVHDQYLEFVTLE+NLFSLAQKS YVQLNDPSAGDREIEEI+E+
Sbjct: 121 DLASGTLNSESIEKISKVHDQYLEFVTLENNLFSLAQKSCYVQLNDPSAGDREIEEIVEK 180

Query: 181 IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHFLSKNNLFTEGGGFASSFQR 240
           + SGLF VLATLAVVPVIRC RGGPAEMVAS LDQ+LRDH LSKNNLFTEGGGFASSFQR
Sbjct: 181 VASGLFCVLATLAVVPVIRCPRGGPAEMVASVLDQKLRDHLLSKNNLFTEGGGFASSFQR 240

Query: 241 PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLSVQGEKGGMKSYELDSSDPFWVA 300
           P+LCLFDRNFELSVGIQHDFRYRPLVHDVLGL+LNRLSVQGEKGGM+SYELDSSDPFWVA
Sbjct: 241 PVLCLFDRNFELSVGIQHDFRYRPLVHDVLGLRLNRLSVQGEKGGMRSYELDSSDPFWVA 300

Query: 301 NGSLEFPEVAVEIETQLNKYKRDVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPE 360
           NGSLEFPEVAVEIETQLNKYK+DVDEVNRRTGGTDGAEFDGTDL+GNTKHLMNAVNSLPE
Sbjct: 301 NGSLEFPEVAVEIETQLNKYKKDVDEVNRRTGGTDGAEFDGTDLVGNTKHLMNAVNSLPE 360

Query: 361 LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGKGTKTD 420
           LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMM RGGIDRNELL VL+GKGTK D
Sbjct: 361 LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMVRGGIDRNELLGVLRGKGTKMD 420

Query: 421 KLRFAVIYLISSETINQSEVEAVETALRESEVDTSAFQYVKKIKSLNVSFSSANSASRSN 480
           KLRFA+IYLI SE+INQSEVEA+ETALRESEVD  AFQYVKK+KSLNVS +SANSASRSN
Sbjct: 421 KLRFAIIYLICSESINQSEVEAIETALRESEVDNCAFQYVKKMKSLNVSLASANSASRSN 480

Query: 481 IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMEGKLNPEIDTFLTFDPRAPK 540
           IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVE LMEGK NPE+D++L FDPRAPK
Sbjct: 481 IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEALMEGKPNPEVDSYLVFDPRAPK 540

Query: 541 SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELAMNQQPVKHIIYGSTEILTGVEFV 600
           S SGTSSSHLKGPFKEAIVFMIGGGNY+EYGSLQE A  QQPVKH+IYG+TEILTG+EFV
Sbjct: 541 SGSGTSSSHLKGPFKEAIVFMIGGGNYMEYGSLQEFAQRQQPVKHVIYGTTEILTGMEFV 600

Query: 601 EQLLLLGQKMGLGNVAAAPPPPGR 625
           EQL +LGQKMGLG+ + APP P R
Sbjct: 601 EQLTVLGQKMGLGS-SVAPPAPTR 622

BLAST of Lsi10G008400 vs. NCBI nr
Match: gi|566167811|ref|XP_006384832.1| (SEC1-family transport family protein [Populus trichocarpa])

HSP 1 Score: 1102.0 bits (2849), Expect = 0.0e+00
Identity = 552/621 (88.89%), Postives = 590/621 (95.01%), Query Frame = 1

Query: 1   MALNLRQKQTECIIRMLNLNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKH 60
           MALNLRQKQTECIIRMLNLNQP+N+T T NEE VYKILI+D+FCQNILSPLIHVKDLRKH
Sbjct: 1   MALNLRQKQTECIIRMLNLNQPLNATGTTNEE-VYKILIYDKFCQNILSPLIHVKDLRKH 60

Query: 61  GITLYFLIDKDRKPVHDVPAVYFVQPSKINIDRIVADASRFLYDSFYLNFSSSIPRPLLE 120
           G+TLYFLIDKDRKPVHDVPAVYFVQPSK+N+ RIVADAS+ LYDSF+LNFSSSIPRPLLE
Sbjct: 61  GVTLYFLIDKDRKPVHDVPAVYFVQPSKVNVQRIVADASQSLYDSFHLNFSSSIPRPLLE 120

Query: 121 DLASGSLNSDSVQRIAKVHDQYLEFVTLEDNLFSLAQKSIYVQLNDPSAGDREIEEIIER 180
           DLASG+LNS+S+ +I+KVHDQYLEFVTLE+NLFSLAQKS YVQLNDPSAGDREIEEI+E+
Sbjct: 121 DLASGTLNSESIDKISKVHDQYLEFVTLENNLFSLAQKSCYVQLNDPSAGDREIEEIVEK 180

Query: 181 IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHFLSKNNLFTEGGGFASSFQR 240
           + SGLFSVLATLAVVPVIRC RGGPAEMVAS LDQ+LRDH LSKNNLFTEGGGFASSFQR
Sbjct: 181 VASGLFSVLATLAVVPVIRCPRGGPAEMVASVLDQKLRDHLLSKNNLFTEGGGFASSFQR 240

Query: 241 PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLSVQGEKGGMKSYELDSSDPFWVA 300
           P+LCLFDRNFELSVGIQHDFRYRPLVHDVLGL+LNRLSVQGEKGGM+SYELDSSDPFWVA
Sbjct: 241 PVLCLFDRNFELSVGIQHDFRYRPLVHDVLGLRLNRLSVQGEKGGMRSYELDSSDPFWVA 300

Query: 301 NGSLEFPEVAVEIETQLNKYKRDVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPE 360
           NGSLEFPEVAVEIETQLNKYK+DVDEVNRRTG TDGAEFDGTDLIGNTKHLMNAVNSLPE
Sbjct: 301 NGSLEFPEVAVEIETQLNKYKKDVDEVNRRTGETDGAEFDGTDLIGNTKHLMNAVNSLPE 360

Query: 361 LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGKGTKTD 420
           LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMM RGGIDRNELL VL+GKGTK D
Sbjct: 361 LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMVRGGIDRNELLGVLRGKGTKID 420

Query: 421 KLRFAVIYLISSETINQSEVEAVETALRESEVDTSAFQYVKKIKSLNVSFSSANSASRSN 480
           KLRFA+IYLI SE+INQSEVEA+ETALRESEVD  AFQYVKK+KSLNVS +SANSASRSN
Sbjct: 421 KLRFAIIYLICSESINQSEVEAIETALRESEVDNCAFQYVKKMKSLNVSLASANSASRSN 480

Query: 481 IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMEGKLNPEIDTFLTFDPRAPK 540
           IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVE LMEGK NPE+D++L FDPRAPK
Sbjct: 481 IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEALMEGKPNPEVDSYLVFDPRAPK 540

Query: 541 SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELAMNQQPVKHIIYGSTEILTGVEFV 600
           S SGTSSSHLKGPFKEAIVFMIGGGNY+EYGSLQE A  QQPVKH+IYG+TEILTG+EFV
Sbjct: 541 SGSGTSSSHLKGPFKEAIVFMIGGGNYMEYGSLQEFAQRQQPVKHVIYGTTEILTGMEFV 600

Query: 601 EQLLLLGQKMGLGNVAAAPPP 622
           EQL +LGQKMGLG+  A P P
Sbjct: 601 EQLTVLGQKMGLGSSVAPPAP 620

BLAST of Lsi10G008400 vs. NCBI nr
Match: gi|743797164|ref|XP_011007325.1| (PREDICTED: SEC1 family transport protein SLY1-like [Populus euphratica])

HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 551/624 (88.30%), Postives = 593/624 (95.03%), Query Frame = 1

Query: 1   MALNLRQKQTECIIRMLNLNQPVNSTSTGNEEEVYKILIFDRFCQNILSPLIHVKDLRKH 60
           MALN RQKQTECIIRMLNLNQP+N+T T NEE VYKILI+D+FCQNILSPLIHVKDLRKH
Sbjct: 1   MALNRRQKQTECIIRMLNLNQPLNATGTTNEE-VYKILIYDKFCQNILSPLIHVKDLRKH 60

Query: 61  GITLYFLIDKDRKPVHDVPAVYFVQPSKINIDRIVADASRFLYDSFYLNFSSSIPRPLLE 120
           G+TLYFLIDKDRKPVHDVPAVYFVQPS++N+ RIVADAS+ LYDSF+LNFSSSIPRPLLE
Sbjct: 61  GVTLYFLIDKDRKPVHDVPAVYFVQPSQVNVQRIVADASQSLYDSFHLNFSSSIPRPLLE 120

Query: 121 DLASGSLNSDSVQRIAKVHDQYLEFVTLEDNLFSLAQKSIYVQLNDPSAGDREIEEIIER 180
           DLASG+LNS+S+++I+KVHDQYLEFVTLE+NLFSLAQKS YVQLNDPSAGDREIEEI+E+
Sbjct: 121 DLASGTLNSESIEKISKVHDQYLEFVTLENNLFSLAQKSCYVQLNDPSAGDREIEEIVEK 180

Query: 181 IVSGLFSVLATLAVVPVIRCQRGGPAEMVASALDQRLRDHFLSKNNLFTEGGGFASSFQR 240
           + SGLF VLATLAVVPVIRC RGGPAEMVAS LDQ+LRDH LSKNNLFTEGGGFASSFQR
Sbjct: 181 VASGLFCVLATLAVVPVIRCPRGGPAEMVASVLDQKLRDHLLSKNNLFTEGGGFASSFQR 240

Query: 241 PILCLFDRNFELSVGIQHDFRYRPLVHDVLGLKLNRLSVQGEKGGMKSYELDSSDPFWVA 300
           P+LCLFDRNFELSVGIQHDFRYRPLVHDVLGL+LNRLSVQGEKGGM+SYELDSSDPFWVA
Sbjct: 241 PVLCLFDRNFELSVGIQHDFRYRPLVHDVLGLRLNRLSVQGEKGGMRSYELDSSDPFWVA 300

Query: 301 NGSLEFPEVAVEIETQLNKYKRDVDEVNRRTGGTDGAEFDGTDLIGNTKHLMNAVNSLPE 360
           NGSLEFPEVAVEIETQLNKYK+DVDEVNRRTGGTDGAEFDGTDL+GNTKHLMNAVNSLPE
Sbjct: 301 NGSLEFPEVAVEIETQLNKYKKDVDEVNRRTGGTDGAEFDGTDLVGNTKHLMNAVNSLPE 360

Query: 361 LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMARGGIDRNELLSVLKGKGTKTD 420
           LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMM RGGIDRNELL VL+GKGTK D
Sbjct: 361 LTERKQVIDKHTNIATVLLGEIKERSLDSYAKKENDMMVRGGIDRNELLGVLRGKGTKMD 420

Query: 421 KLRFAVIYLISSETINQSEVEAVETALRESEVDTSAFQYVKKIKSLNVSFSSANSASRSN 480
           KLRFA+IYLI SE+INQSEVEA+ETALRESEVD  AFQYVKK+KSLNVS +SANSASRSN
Sbjct: 421 KLRFAIIYLICSESINQSEVEAIETALRESEVDNCAFQYVKKMKSLNVSLASANSASRSN 480

Query: 481 IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEGLMEGKLNPEIDTFLTFDPRAPK 540
           IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVE LMEGK NPE+D++L FDPRAPK
Sbjct: 481 IVDWAEKLYGQSISAVTAGVKNLLSSDRQLALTRTVEALMEGKPNPEVDSYLVFDPRAPK 540

Query: 541 SSSGTSSSHLKGPFKEAIVFMIGGGNYVEYGSLQELAMNQQPVKHIIYGSTEILTGVEFV 600
           S SGTSSSHLKGPFKEAIVFMIGGGNY+EYGSLQE A  QQPVKH+IYG+TEILTG+EFV
Sbjct: 541 SGSGTSSSHLKGPFKEAIVFMIGGGNYMEYGSLQEFAQRQQPVKHVIYGTTEILTGMEFV 600

Query: 601 EQLLLLGQKMGLGNVAAAPPPPGR 625
           EQL +LGQKMGLG+ + APP P R
Sbjct: 601 EQLTVLGQKMGLGS-SVAPPAPTR 622

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SLY1_ARATH3.7e-28479.58SEC1 family transport protein SLY1 OS=Arabidopsis thaliana GN=SLY1 PE=1 SV=1[more]
SLY1_ORYSJ6.2e-21562.03SEC1 family transport protein SLY1 OS=Oryza sativa subsp. japonica GN=SLY1 PE=2 ... [more]
SCFD1_HUMAN3.3e-12340.41Sec1 family domain-containing protein 1 OS=Homo sapiens GN=SCFD1 PE=1 SV=4[more]
SCFD1_RAT3.3e-12340.97Sec1 family domain-containing protein 1 OS=Rattus norvegicus GN=Scfd1 PE=1 SV=1[more]
SCFD1_MOUSE7.2e-12341.17Sec1 family domain-containing protein 1 OS=Mus musculus GN=Scfd1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KSS4_CUCSA0.0e+0096.31Uncharacterized protein OS=Cucumis sativus GN=Csa_5G265810 PE=3 SV=1[more]
B9MYY5_POPTR0.0e+0088.89SEC1-family transport family protein OS=Populus trichocarpa GN=POPTR_0004s21450g... [more]
A0A061E5A4_THECC0.0e+0088.65Sec1/munc18-like (SM) proteins superfamily OS=Theobroma cacao GN=TCM_006465 PE=3... [more]
A0A0D2TWT9_GOSRA0.0e+0088.03Uncharacterized protein OS=Gossypium raimondii GN=B456_008G040800 PE=3 SV=1[more]
A0A0B0NR49_GOSAR0.0e+0088.03SEC1 family transport protein SLY1 OS=Gossypium arboreum GN=F383_06667 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G17980.12.1e-28579.58 Sec1/munc18-like (SM) proteins superfamily[more]
AT4G31740.16.6e-5864.77 Sec1/munc18-like (SM) proteins superfamily[more]
AT4G36100.11.3e-3462.04 Sec1/munc18-like (SM) proteins superfamily[more]
AT1G12360.19.7e-2522.81 Sec1/munc18-like (SM) proteins superfamily[more]
AT1G02010.18.2e-2423.00 secretory 1A[more]
Match NameE-valueIdentityDescription
gi|449445286|ref|XP_004140404.1|0.0e+0096.31PREDICTED: SEC1 family transport protein SLY1-like [Cucumis sativus][more]
gi|659120511|ref|XP_008460227.1|0.0e+0096.15PREDICTED: SEC1 family transport protein SLY1 [Cucumis melo][more]
gi|743895028|ref|XP_011040777.1|0.0e+0088.46PREDICTED: SEC1 family transport protein SLY1-like [Populus euphratica][more]
gi|566167811|ref|XP_006384832.1|0.0e+0088.89SEC1-family transport family protein [Populus trichocarpa][more]
gi|743797164|ref|XP_011007325.1|0.0e+0088.30PREDICTED: SEC1 family transport protein SLY1-like [Populus euphratica][more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0016192vesicle-mediated transport
GO:0006904vesicle docking involved in exocytosis
Vocabulary: INTERPRO
TermDefinition
IPR027482Sec-1-like_dom2
IPR001619Sec1-like
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006904 vesicle docking involved in exocytosis
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0005575 cellular_component
cellular_component GO:0005829 cytosol
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G008400.1Lsi10G008400.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001619Sec1-like proteinPIRPIRSF005715VPS45_Sec1coord: 3..614
score: 4.8
IPR001619Sec1-like proteinPANTHERPTHR11679VESICLE PROTEIN SORTING-ASSOCIATEDcoord: 2..612
score: 2.9E
IPR001619Sec1-like proteinPFAMPF00995Sec1coord: 36..602
score: 6.9
IPR001619Sec1-like proteinunknownSSF56815Sec1/munc18-like (SM) proteinscoord: 1..609
score: 4.71E
IPR027482Sec1-like, domain 2GENE3DG3DSA:3.40.50.1910coord: 511..611
score: 7.2E-66coord: 145..258
score: 7.2
NoneNo IPR availableunknownCoilCoilcoord: 310..330
scor
NoneNo IPR availableGENE3DG3DSA:1.25.40.60coord: 395..494
score: 4.2
NoneNo IPR availableGENE3DG3DSA:3.40.50.2060coord: 4..140
score: 4.6
NoneNo IPR availableGENE3DG3DSA:3.90.830.10coord: 260..394
score: 2.9
NoneNo IPR availablePANTHERPTHR11679:SF2SEC1 FAMILY DOMAIN-CONTAINING PROTEIN 1coord: 2..612
score: 2.9E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi10G008400CmaCh02G002940Cucurbita maxima (Rimu)cmalsiB556
Lsi10G008400CmoCh02G002890Cucurbita moschata (Rifu)cmolsiB541
Lsi10G008400Carg19763Silver-seed gourdcarlsiB778
The following gene(s) are paralogous to this gene:

None