Lsi04G004780.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi04G004780.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Protein NRT1/ PTR FAMILY 2.13
Location	chr04 : 4455071 .. 4455640 (+)
Sequence length	570

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACACAGGGGATGGCTTCAATGACACTGACGGCGTGGTTGCCACAGCTACATCCGCCGGGTTGCAGCGGATCAACGAAATGCTCAGGGCCGACAGCTTCCCAATTGGGATTCTTAGTAATGAGTCTTTGTTTAGTATCAATCGGAAGCGGCGGAATCAGGCCGTGTAGTATTCCATTTGGGGTAGACCAATTTGACCCGACGACGGAGAAAGGGAGGACAGGGATAGCGAGTTTTTACAATTGGTATTACGCGACTTTCATGGTGGTTTTGGTGTTTACTTTGACGATTGTTGTGTATATTCAAGATTCAGTGAGTTGGGTTTTGGGATATGGAATTCCGACGGGGCTTATGTTTTGTTCTATTGTTCTGTTCTTCGTGGGGACTCGTGTTTATGTGTTGATAAAGCCTGAAGGAAGCATATTTACTGGGTTAGCTCAAGTGGCTGTTGCTGCTTACAAAAAGCGTCATCTTAAGCTTCCTGATGATAGGATGGGTTTTTATGACCCTCCCTTGAACCCTGACTCTGTTGTCCTCTCCAAGCTTCCTCTCACCAACCAATTCAGGTAA

mRNA sequence

ATGACACAGGGGATGGCTTCAATGACACTGACGGCGTGGTTGCCACAGCTACATCCGCCGGGTTGCAGCGGATCAACGAAATGCTCAGGGCCGACAGCTTCCCAATTGGGATTCTTAGTAATGAGTCTTTGTTTAGTATCAATCGGAAGCGGCGGAATCAGGCCGTGTAGTATTCCATTTGGGGTAGACCAATTTGACCCGACGACGGAGAAAGGGAGGACAGGGATAGCGAGTTTTTACAATTGGTATTACGCGACTTTCATGGTGGTTTTGGTGTTTACTTTGACGATTGTTGTGTATATTCAAGATTCAGTGAGTTGGGTTTTGGGATATGGAATTCCGACGGGGCTTATGTTTTGTTCTATTGTTCTGTTCTTCGTGGGGACTCGTGTTTATGTGTTGATAAAGCCTGAAGGAAGCATATTTACTGGGTTAGCTCAAGTGGCTGTTGCTGCTTACAAAAAGCGTCATCTTAAGCTTCCTGATGATAGGATGGGTTTTTATGACCCTCCCTTGAACCCTGACTCTGTTGTCCTCTCCAAGCTTCCTCTCACCAACCAATTCAGGTAA

Coding sequence (CDS)

ATGACACAGGGGATGGCTTCAATGACACTGACGGCGTGGTTGCCACAGCTACATCCGCCGGGTTGCAGCGGATCAACGAAATGCTCAGGGCCGACAGCTTCCCAATTGGGATTCTTAGTAATGAGTCTTTGTTTAGTATCAATCGGAAGCGGCGGAATCAGGCCGTGTAGTATTCCATTTGGGGTAGACCAATTTGACCCGACGACGGAGAAAGGGAGGACAGGGATAGCGAGTTTTTACAATTGGTATTACGCGACTTTCATGGTGGTTTTGGTGTTTACTTTGACGATTGTTGTGTATATTCAAGATTCAGTGAGTTGGGTTTTGGGATATGGAATTCCGACGGGGCTTATGTTTTGTTCTATTGTTCTGTTCTTCGTGGGGACTCGTGTTTATGTGTTGATAAAGCCTGAAGGAAGCATATTTACTGGGTTAGCTCAAGTGGCTGTTGCTGCTTACAAAAAGCGTCATCTTAAGCTTCCTGATGATAGGATGGGTTTTTATGACCCTCCCTTGAACCCTGACTCTGTTGTCCTCTCCAAGCTTCCTCTCACCAACCAATTCAGGTAA

Protein sequence

MTQGMASMTLTAWLPQLHPPGCSGSTKCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIPFGVDQFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCSIVLFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRMGFYDPPLNPDSVVLSKLPLTNQFR

Homology

[Close]

BLAST of Lsi04G004780.1 vs. Swiss-Prot
Match: PTR21_ARATH (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 8.5e-59
Identity = 113/191 (59.16%), Postives = 141/191 (73.82%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSGST--KCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIPFG 63
           G+ ++TLTA  PQLHP  C+      C GP   Q+G L++ LC +S+GSGGIRPCSIPFG
Sbjct: 136 GLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFG 195

Query: 64  VDQFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCS 123
           VDQFD  TE+G  G+ASF+NWYY TF VVL+ T T+VVYIQD VSW++G+ IPTGLM  +
Sbjct: 196 VDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQTVVVYIQDQVSWIIGFSIPTGLMALA 255

Query: 124 IVLFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLKLP---DDRMGFYDPPLNPDSVV 183
           +V+FF G + YV +KPEGSIF+G+AQV VAA KKR LKLP   D  + +YDP +   S V
Sbjct: 256 VVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKLKLPAEDDGTVTYYDPAIK--SSV 315

Query: 184 LSKLPLTNQFR 190
           LSKL  +NQFR
Sbjct: 316 LSKLHRSNQFR 324

[Close]

BLAST of Lsi04G004780.1 vs. Swiss-Prot
Match: PTR13_ARATH (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 221.1 bits (562), Expect = 1.0e-56
Identity = 112/191 (58.64%), Postives = 143/191 (74.87%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSGS--TKCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIPFG 63
           G+ ++TLTA LPQLHPP C+     +C  P   QLG L + L  +SIGSGGIRPCSIPFG
Sbjct: 99  GLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGILFLGLGFLSIGSGGIRPCSIPFG 158

Query: 64  VDQFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCS 123
           VDQFD  TE+G  G+ASF+NWYY T  +VL+F+ T+VVY+Q +VSWV+G+ IPT LM C+
Sbjct: 159 VDQFDQRTEQGLKGVASFFNWYYLTLTMVLIFSHTVVVYLQ-TVSWVIGFSIPTSLMACA 218

Query: 124 IVLFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLKL---PDDRMGFYDPPLNPDSVV 183
           +VLFFVG R YV +KPEGS+F+G+A+V VAA KKR LK+    D    +Y+PP+ P   V
Sbjct: 219 VVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRDLKISLVDDGTEEYYEPPVKPG--V 278

Query: 184 LSKLPLTNQFR 190
           LSKLPLT+QF+
Sbjct: 279 LSKLPLTDQFK 286

[Close]

BLAST of Lsi04G004780.1 vs. Swiss-Prot
Match: PTR20_ARATH (Putative protein NRT1/ PTR FAMILY 2.14 OS=Arabidopsis thaliana GN=NPF2.14 PE=5 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 3.2e-42
Identity = 87/189 (46.03%), Postives = 130/189 (68.78%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSGST---KCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIPF 63
           GM  +T T+ +P L PP C+      +C   + SQL  L+  L L+S+G+GGIR CS+PF
Sbjct: 103 GMLVLTFTSLVPNLRPPPCTADQITGQCIPYSYSQLYVLLSGLFLLSVGTGGIRSCSVPF 162

Query: 64  GVDQFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFC 123
            +DQFD +TE+GR G  SF++WYY T  +V + ++T+V+Y+Q+++SW +G+ IPT L F 
Sbjct: 163 SLDQFDDSTEEGREGSRSFFSWYYTTHTIVQLVSMTLVLYVQNNISWGIGFAIPTVLNFF 222

Query: 124 SIVLFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRMGFYDPPLNPDSVVLS 183
           +++L FVGTR YV +KPEGS+F+G+ +V VAAYKKR  +     + ++ P L  D +  +
Sbjct: 223 ALLLLFVGTRYYVFVKPEGSVFSGVFKVLVAAYKKRKARFTSG-IDYHQPLLETD-LQSN 282

Query: 184 KLPLTNQFR 190
           KL LT+QFR
Sbjct: 283 KLVLTDQFR 289

[Close]

BLAST of Lsi04G004780.1 vs. Swiss-Prot
Match: PTR53_ARATH (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 6.8e-40
Identity = 85/189 (44.97%), Postives = 118/189 (62.43%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGC--SGSTKCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIPFG 63
           G   + LTA +PQLHP  C  +  + C+GP+  Q+ FL+M L  + +G+GGIRPC++ FG
Sbjct: 128 GSFVILLTAAVPQLHPAACGTAADSICNGPSGGQIAFLLMGLGFLVVGAGGIRPCNLAFG 187

Query: 64  VDQFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCS 123
            DQF+P +E G+ GI SF+NWY+ TF    + +LT+VVY+Q +VSW +G  IP  LMF +
Sbjct: 188 ADQFNPKSESGKRGIDSFFNWYFFTFTFAQILSLTLVVYVQSNVSWTIGLTIPAVLMFLA 247

Query: 124 IVLFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLK-LPDDRMGFYDPPLNPDSVVLS 183
            ++FF G ++YV IK  GS   G+AQV   A KKR LK      +  Y+    P     S
Sbjct: 248 CLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIKKRGLKPAKQPWLNLYN--YYPPKYANS 307

Query: 184 KLPLTNQFR 190
           KL  T+QFR
Sbjct: 308 KLKYTDQFR 314

[Close]

BLAST of Lsi04G004780.1 vs. Swiss-Prot
Match: PTR44_ARATH (Protein NRT1/ PTR FAMILY 2.10 OS=Arabidopsis thaliana GN=NPF2.10 PE=1 SV=3)

HSP 1 Score: 164.5 bits (415), Expect = 1.2e-39
Identity = 84/187 (44.92%), Postives = 115/187 (61.50%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSGSTKCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIPFGVD 63
           G   + LTA +P LHP  C     C GP+  Q+ FL+M L  + +G+GGIRPC++ FG D
Sbjct: 146 GSFVILLTAAIPSLHPVACGNKISCEGPSVGQILFLLMGLGFLVVGAGGIRPCNLAFGAD 205

Query: 64  QFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCSIV 123
           QF+P +E G+ GI SF+NWY+ TF    + +LT VVYIQ +VSW +G  IP  LMF + V
Sbjct: 206 QFNPKSESGKKGINSFFNWYFFTFTFAQIISLTAVVYIQSNVSWTIGLIIPVALMFLACV 265

Query: 124 LFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLK-LPDDRMGFYDPPLNPDSVVLSKL 183
           +FF G R+YV +K  GS   G+A+V  AA KKR LK +    +  Y+    P +   + L
Sbjct: 266 IFFAGDRLYVKVKASGSPLAGIARVIAAAIKKRGLKPVKQPWVNLYNH--IPSNYANTTL 325

Query: 184 PLTNQFR 190
             T+QFR
Sbjct: 326 KYTDQFR 330

[Close]

BLAST of Lsi04G004780.1 vs. TrEMBL
Match: A0A0A0KTW3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G604170 PE=3 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 2.8e-93
Identity = 170/185 (91.89%), Postives = 174/185 (94.05%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSGSTKCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIPFGVD 63
           GMASMTLTAWLPQLHPPGC GS KC  PTASQLGFL+MSLCLVSIGSGGIRPCSIPFGVD
Sbjct: 123 GMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVD 182

Query: 64  QFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCSIV 123
           QFDPTTEKGR GIASFYNWYYATFMVVLV TLTIVVYIQDSVSWVLGYGIPT LMFCSI+
Sbjct: 183 QFDPTTEKGRKGIASFYNWYYATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCSII 242

Query: 124 LFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRMGFYDPPLNPDSVVLSKLP 183
           LFF+GT VYV IKPEGSIFTGLAQVAVAAYKKRHLKLPDDR GFYDPPL+P SVVLSKLP
Sbjct: 243 LFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLSKLP 302

Query: 184 LTNQF 189
           LTNQF
Sbjct: 303 LTNQF 307

[Close]

BLAST of Lsi04G004780.1 vs. TrEMBL
Match: U5G3E2_POPTR (Proton-dependent oligopeptide transport family protein OS=Populus trichocarpa GN=POPTR_0010s04370g PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.1e-65
Identity = 125/198 (63.13%), Postives = 151/198 (76.26%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSGSTK------CSGPTASQLGFLVMSLCLVSIGSGGIRPCS 63
           GM ++T TAW+P LHP  C    K      C  PT+ QLG L++ L  +SIG+GGIRPCS
Sbjct: 126 GMVTVTSTAWVPYLHPQKCEPEGKQQSYGNCESPTSMQLGVLLLGLGFLSIGTGGIRPCS 185

Query: 64  IPFGVDQFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGL 123
           IPFGVDQFDPTTE+G  GI+S+YNWYY TF VV++ TLT VVY+QDSVSWVLG+GIPT L
Sbjct: 186 IPFGVDQFDPTTEEGIKGISSYYNWYYTTFTVVMLITLTAVVYVQDSVSWVLGFGIPTVL 245

Query: 124 MFCSIVLFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRMG------FYDPP 183
           M CSI+LFF+GTR+YV +KPEGS+F+G+AQV V+AYKKR LKLPD+  G      FYDPP
Sbjct: 246 MLCSIILFFIGTRIYVHVKPEGSVFSGIAQVFVSAYKKRRLKLPDNCDGEQVDGIFYDPP 305

Query: 184 LNPDSVVLSKLPLTNQFR 190
           +     +LSKLPLTNQ R
Sbjct: 306 IKDQLTILSKLPLTNQIR 323

[Close]

BLAST of Lsi04G004780.1 vs. TrEMBL
Match: A0A022QN29_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a004515mg PE=4 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 2.5e-65
Identity = 126/190 (66.32%), Postives = 150/190 (78.95%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSG--STKCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIPFG 63
           GM +MTL AW+PQLHPP C    S +C GPT SQLG L ++L  +SIGSGGIRPCSIPFG
Sbjct: 44  GMVTMTLIAWIPQLHPPPCKNLQSNQCLGPTKSQLGVLAVALGFLSIGSGGIRPCSIPFG 103

Query: 64  VDQFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCS 123
           VDQFD  TE+GR GI SF+NWYY TF +VL+  LT+VVYIQDSVSWV G+G+PT LM CS
Sbjct: 104 VDQFDSNTEEGRRGINSFFNWYYTTFTLVLIIALTLVVYIQDSVSWVWGFGLPTALMLCS 163

Query: 124 IVLFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRM--GFYDPPLNPDSVVL 183
           I+LFFVGTR+YV +KPEGS+F+G+ QV VAAYKKR  KLP+D +   +YDPP    +VV 
Sbjct: 164 ILLFFVGTRLYVYVKPEGSVFSGIGQVFVAAYKKRRQKLPEDYVDGAYYDPPPLKGTVV- 223

Query: 184 SKLPLTNQFR 190
            KLPLTNQ+R
Sbjct: 224 KKLPLTNQYR 232

[Close]

BLAST of Lsi04G004780.1 vs. TrEMBL
Match: D7TNF6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0026g01490 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 8.0e-64
Identity = 121/193 (62.69%), Postives = 155/193 (80.31%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSG----STKCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIP 63
           GM ++TLT+W+PQLHPP C+     + +C+GPT++Q+G L + L  ++IG+GGIRPCSIP
Sbjct: 137 GMVTLTLTSWIPQLHPPSCTEDQQQNDQCTGPTSAQMGILFLGLGFLTIGTGGIRPCSIP 196

Query: 64  FGVDQFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMF 123
           FGVDQFDPTTE+GR GI SFYNWYY +F +VL+  LT+VVYIQDSVSWVLG+GIPT LM 
Sbjct: 197 FGVDQFDPTTEQGRRGINSFYNWYYTSFTLVLMIALTLVVYIQDSVSWVLGFGIPTLLMV 256

Query: 124 CSIVLFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLKLP---DDRMGFYDPPLNPDS 183
            SI+LFF+GTRVYV +KPEGSIF+G+A+V VAAYKKR +K+P   +    +YDPPL   S
Sbjct: 257 GSIILFFLGTRVYVYVKPEGSIFSGIARVFVAAYKKRRVKIPVEGEKEGAYYDPPLTKGS 316

Query: 184 VVLSKLPLTNQFR 190
           +V +KLP T +FR
Sbjct: 317 IV-AKLPFTKRFR 328

[Close]

BLAST of Lsi04G004780.1 vs. TrEMBL
Match: A0A067JBR4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21712 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 1.4e-63
Identity = 126/190 (66.32%), Postives = 148/190 (77.89%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSGSTKCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIPFGVD 63
           GM ++TLTAWLP LHPP C     C  PTA+QLG L M L L++IG+GGIRPCSIPFGVD
Sbjct: 138 GMVTVTLTAWLPYLHPPKCQQDINCESPTAAQLGVLFMGLGLLTIGTGGIRPCSIPFGVD 197

Query: 64  QFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCSIV 123
           QFDPTTE+G  GI SFYNWYY TF VV++ T T+VVYIQDSVSWVLG+GIPT LM  S+ 
Sbjct: 198 QFDPTTEEGIKGINSFYNWYYTTFTVVIMITSTVVVYIQDSVSWVLGFGIPTLLMIGSLA 257

Query: 124 LFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLKLPDD-RMG---FYDPPLNPDSVVL 183
           LFF+G R+YV +KPEGSIF+ + QV VAAY+KR L+LPD+  +G   FYDPP   +   L
Sbjct: 258 LFFLGQRIYVYVKPEGSIFSSIFQVFVAAYRKRGLQLPDEGEIGCRVFYDPPTKVNE--L 317

Query: 184 SKLPLTNQFR 190
           SKLPLTNQFR
Sbjct: 318 SKLPLTNQFR 325

[Close]

BLAST of Lsi04G004780.1 vs. TAIR10
Match: AT1G69870.1 (AT1G69870.1 nitrate transporter 1.7)

HSP 1 Score: 228.0 bits (580), Expect = 4.8e-60
Identity = 113/191 (59.16%), Postives = 141/191 (73.82%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSGST--KCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIPFG 63
           G+ ++TLTA  PQLHP  C+      C GP   Q+G L++ LC +S+GSGGIRPCSIPFG
Sbjct: 136 GLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGVLLLGLCFLSVGSGGIRPCSIPFG 195

Query: 64  VDQFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCS 123
           VDQFD  TE+G  G+ASF+NWYY TF VVL+ T T+VVYIQD VSW++G+ IPTGLM  +
Sbjct: 196 VDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQTVVVYIQDQVSWIIGFSIPTGLMALA 255

Query: 124 IVLFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLKLP---DDRMGFYDPPLNPDSVV 183
           +V+FF G + YV +KPEGSIF+G+AQV VAA KKR LKLP   D  + +YDP +   S V
Sbjct: 256 VVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKLKLPAEDDGTVTYYDPAIK--SSV 315

Query: 184 LSKLPLTNQFR 190
           LSKL  +NQFR
Sbjct: 316 LSKLHRSNQFR 324

[Close]

BLAST of Lsi04G004780.1 vs. TAIR10
Match: AT1G27080.1 (AT1G27080.1 nitrate transporter 1.6)

HSP 1 Score: 221.1 bits (562), Expect = 5.9e-58
Identity = 112/191 (58.64%), Postives = 143/191 (74.87%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSGS--TKCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIPFG 63
           G+ ++TLTA LPQLHPP C+     +C  P   QLG L + L  +SIGSGGIRPCSIPFG
Sbjct: 99  GLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGILFLGLGFLSIGSGGIRPCSIPFG 158

Query: 64  VDQFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCS 123
           VDQFD  TE+G  G+ASF+NWYY T  +VL+F+ T+VVY+Q +VSWV+G+ IPT LM C+
Sbjct: 159 VDQFDQRTEQGLKGVASFFNWYYLTLTMVLIFSHTVVVYLQ-TVSWVIGFSIPTSLMACA 218

Query: 124 IVLFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLKL---PDDRMGFYDPPLNPDSVV 183
           +VLFFVG R YV +KPEGS+F+G+A+V VAA KKR LK+    D    +Y+PP+ P   V
Sbjct: 219 VVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRDLKISLVDDGTEEYYEPPVKPG--V 278

Query: 184 LSKLPLTNQFR 190
           LSKLPLT+QF+
Sbjct: 279 LSKLPLTDQFK 286

[Close]

BLAST of Lsi04G004780.1 vs. TAIR10
Match: AT1G69860.1 (AT1G69860.1 Major facilitator superfamily protein)

HSP 1 Score: 172.9 bits (437), Expect = 1.8e-43
Identity = 87/189 (46.03%), Postives = 130/189 (68.78%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSGST---KCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIPF 63
           GM  +T T+ +P L PP C+      +C   + SQL  L+  L L+S+G+GGIR CS+PF
Sbjct: 103 GMLVLTFTSLVPNLRPPPCTADQITGQCIPYSYSQLYVLLSGLFLLSVGTGGIRSCSVPF 162

Query: 64  GVDQFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFC 123
            +DQFD +TE+GR G  SF++WYY T  +V + ++T+V+Y+Q+++SW +G+ IPT L F 
Sbjct: 163 SLDQFDDSTEEGREGSRSFFSWYYTTHTIVQLVSMTLVLYVQNNISWGIGFAIPTVLNFF 222

Query: 124 SIVLFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRMGFYDPPLNPDSVVLS 183
           +++L FVGTR YV +KPEGS+F+G+ +V VAAYKKR  +     + ++ P L  D +  +
Sbjct: 223 ALLLLFVGTRYYVFVKPEGSVFSGVFKVLVAAYKKRKARFTSG-IDYHQPLLETD-LQSN 282

Query: 184 KLPLTNQFR 190
           KL LT+QFR
Sbjct: 283 KLVLTDQFR 289

[Close]

BLAST of Lsi04G004780.1 vs. TAIR10
Match: AT5G62680.1 (AT5G62680.1 Major facilitator superfamily protein)

HSP 1 Score: 165.2 bits (417), Expect = 3.8e-41
Identity = 85/189 (44.97%), Postives = 118/189 (62.43%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGC--SGSTKCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIPFG 63
           G   + LTA +PQLHP  C  +  + C+GP+  Q+ FL+M L  + +G+GGIRPC++ FG
Sbjct: 128 GSFVILLTAAVPQLHPAACGTAADSICNGPSGGQIAFLLMGLGFLVVGAGGIRPCNLAFG 187

Query: 64  VDQFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCS 123
            DQF+P +E G+ GI SF+NWY+ TF    + +LT+VVY+Q +VSW +G  IP  LMF +
Sbjct: 188 ADQFNPKSESGKRGIDSFFNWYFFTFTFAQILSLTLVVYVQSNVSWTIGLTIPAVLMFLA 247

Query: 124 IVLFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLK-LPDDRMGFYDPPLNPDSVVLS 183
            ++FF G ++YV IK  GS   G+AQV   A KKR LK      +  Y+    P     S
Sbjct: 248 CLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIKKRGLKPAKQPWLNLYN--YYPPKYANS 307

Query: 184 KLPLTNQFR 190
           KL  T+QFR
Sbjct: 308 KLKYTDQFR 314

[Close]

BLAST of Lsi04G004780.1 vs. TAIR10
Match: AT3G47960.1 (AT3G47960.1 Major facilitator superfamily protein)

HSP 1 Score: 164.5 bits (415), Expect = 6.5e-41
Identity = 84/187 (44.92%), Postives = 115/187 (61.50%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSGSTKCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIPFGVD 63
           G   + LTA +P LHP  C     C GP+  Q+ FL+M L  + +G+GGIRPC++ FG D
Sbjct: 146 GSFVILLTAAIPSLHPVACGNKISCEGPSVGQILFLLMGLGFLVVGAGGIRPCNLAFGAD 205

Query: 64  QFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCSIV 123
           QF+P +E G+ GI SF+NWY+ TF    + +LT VVYIQ +VSW +G  IP  LMF + V
Sbjct: 206 QFNPKSESGKKGINSFFNWYFFTFTFAQIISLTAVVYIQSNVSWTIGLIIPVALMFLACV 265

Query: 124 LFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLK-LPDDRMGFYDPPLNPDSVVLSKL 183
           +FF G R+YV +K  GS   G+A+V  AA KKR LK +    +  Y+    P +   + L
Sbjct: 266 IFFAGDRLYVKVKASGSPLAGIARVIAAAIKKRGLKPVKQPWVNLYNH--IPSNYANTTL 325

Query: 184 PLTNQFR 190
             T+QFR
Sbjct: 326 KYTDQFR 330

[Close]

BLAST of Lsi04G004780.1 vs. NCBI nr
Match: gi|659090965|ref|XP_008446297.1| (PREDICTED: LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 2.13-like [Cucumis melo])

HSP 1 Score: 355.1 bits (910), Expect = 7.4e-95
Identity = 172/185 (92.97%), Postives = 176/185 (95.14%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSGSTKCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIPFGVD 63
           GMASMTLTAWLPQLHPPGC GS KC  PTASQLGFL+MSLCLVSIGSGGIRPCSIPFGVD
Sbjct: 123 GMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVD 182

Query: 64  QFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCSIV 123
           QFDPTTEKGR GIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCSI+
Sbjct: 183 QFDPTTEKGRKGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCSII 242

Query: 124 LFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRMGFYDPPLNPDSVVLSKLP 183
           LFF+GT VYV IKPEGSIFTGLAQVAVAAYKKRHLKLPDDR GFYDPPL+P SVVLSKLP
Sbjct: 243 LFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLSKLP 302

Query: 184 LTNQF 189
           LTNQF
Sbjct: 303 LTNQF 307

[Close]

BLAST of Lsi04G004780.1 vs. NCBI nr
Match: gi|449434837|ref|XP_004135202.1| (PREDICTED: protein NRT1/ PTR FAMILY 2.13-like [Cucumis sativus])

HSP 1 Score: 349.4 bits (895), Expect = 4.0e-93
Identity = 170/185 (91.89%), Postives = 174/185 (94.05%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSGSTKCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIPFGVD 63
           GMASMTLTAWLPQLHPPGC GS KC  PTASQLGFL+MSLCLVSIGSGGIRPCSIPFGVD
Sbjct: 123 GMASMTLTAWLPQLHPPGCGGSVKCLTPTASQLGFLIMSLCLVSIGSGGIRPCSIPFGVD 182

Query: 64  QFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCSIV 123
           QFDPTTEKGR GIASFYNWYYATFMVVLV TLTIVVYIQDSVSWVLGYGIPT LMFCSI+
Sbjct: 183 QFDPTTEKGRKGIASFYNWYYATFMVVLVITLTIVVYIQDSVSWVLGYGIPTVLMFCSII 242

Query: 124 LFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRMGFYDPPLNPDSVVLSKLP 183
           LFF+GT VYV IKPEGSIFTGLAQVAVAAYKKRHLKLPDDR GFYDPPL+P SVVLSKLP
Sbjct: 243 LFFMGTHVYVFIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRKGFYDPPLDPASVVLSKLP 302

Query: 184 LTNQF 189
           LTNQF
Sbjct: 303 LTNQF 307

[Close]

BLAST of Lsi04G004780.1 vs. NCBI nr
Match: gi|743924907|ref|XP_011006584.1| (PREDICTED: protein NRT1/ PTR FAMILY 2.13 [Populus euphratica])

HSP 1 Score: 260.4 bits (664), Expect = 2.5e-66
Identity = 126/198 (63.64%), Postives = 151/198 (76.26%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSGSTK------CSGPTASQLGFLVMSLCLVSIGSGGIRPCS 63
           GM ++TLTAW+P LHP  C    K      C  PT+ Q G L++ L  +SIG+GGIRPCS
Sbjct: 126 GMVTVTLTAWVPYLHPKKCEPEGKQQSYGNCESPTSMQFGVLLLGLGFLSIGTGGIRPCS 185

Query: 64  IPFGVDQFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGL 123
           IPFGVDQFDPTTE+GR GI+SFYNWYY +F VVL+ TLT VVY+QDSVSWVLG+GIPT L
Sbjct: 186 IPFGVDQFDPTTEEGRKGISSFYNWYYTSFTVVLLITLTAVVYVQDSVSWVLGFGIPTVL 245

Query: 124 MFCSIVLFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRMG------FYDPP 183
           M CSI+LFF+GTR+YV +KPEGS+F+G+ QV V+AYKKR LKLPD+  G      FYDPP
Sbjct: 246 MLCSIILFFIGTRIYVHVKPEGSVFSGITQVFVSAYKKRRLKLPDNCDGEQVDGIFYDPP 305

Query: 184 LNPDSVVLSKLPLTNQFR 190
           +     +LSKLPLTNQ R
Sbjct: 306 IKDQLTILSKLPLTNQIR 323

[Close]

BLAST of Lsi04G004780.1 vs. NCBI nr
Match: gi|566189009|ref|XP_006378165.1| (proton-dependent oligopeptide transport family protein [Populus trichocarpa])

HSP 1 Score: 257.7 bits (657), Expect = 1.6e-65
Identity = 125/198 (63.13%), Postives = 151/198 (76.26%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSGSTK------CSGPTASQLGFLVMSLCLVSIGSGGIRPCS 63
           GM ++T TAW+P LHP  C    K      C  PT+ QLG L++ L  +SIG+GGIRPCS
Sbjct: 126 GMVTVTSTAWVPYLHPQKCEPEGKQQSYGNCESPTSMQLGVLLLGLGFLSIGTGGIRPCS 185

Query: 64  IPFGVDQFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGL 123
           IPFGVDQFDPTTE+G  GI+S+YNWYY TF VV++ TLT VVY+QDSVSWVLG+GIPT L
Sbjct: 186 IPFGVDQFDPTTEEGIKGISSYYNWYYTTFTVVMLITLTAVVYVQDSVSWVLGFGIPTVL 245

Query: 124 MFCSIVLFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRMG------FYDPP 183
           M CSI+LFF+GTR+YV +KPEGS+F+G+AQV V+AYKKR LKLPD+  G      FYDPP
Sbjct: 246 MLCSIILFFIGTRIYVHVKPEGSVFSGIAQVFVSAYKKRRLKLPDNCDGEQVDGIFYDPP 305

Query: 184 LNPDSVVLSKLPLTNQFR 190
           +     +LSKLPLTNQ R
Sbjct: 306 IKDQLTILSKLPLTNQIR 323

[Close]

BLAST of Lsi04G004780.1 vs. NCBI nr
Match: gi|604316501|gb|EYU28693.1| (hypothetical protein MIMGU_mgv1a004515mg [Erythranthe guttata])

HSP 1 Score: 256.5 bits (654), Expect = 3.6e-65
Identity = 126/190 (66.32%), Postives = 150/190 (78.95%), Query Frame = 1


Query: 4   GMASMTLTAWLPQLHPPGCSG--STKCSGPTASQLGFLVMSLCLVSIGSGGIRPCSIPFG 63
           GM +MTL AW+PQLHPP C    S +C GPT SQLG L ++L  +SIGSGGIRPCSIPFG
Sbjct: 44  GMVTMTLIAWIPQLHPPPCKNLQSNQCLGPTKSQLGVLAVALGFLSIGSGGIRPCSIPFG 103

Query: 64  VDQFDPTTEKGRTGIASFYNWYYATFMVVLVFTLTIVVYIQDSVSWVLGYGIPTGLMFCS 123
           VDQFD  TE+GR GI SF+NWYY TF +VL+  LT+VVYIQDSVSWV G+G+PT LM CS
Sbjct: 104 VDQFDSNTEEGRRGINSFFNWYYTTFTLVLIIALTLVVYIQDSVSWVWGFGLPTALMLCS 163

Query: 124 IVLFFVGTRVYVLIKPEGSIFTGLAQVAVAAYKKRHLKLPDDRM--GFYDPPLNPDSVVL 183
           I+LFFVGTR+YV +KPEGS+F+G+ QV VAAYKKR  KLP+D +   +YDPP    +VV 
Sbjct: 164 ILLFFVGTRLYVYVKPEGSVFSGIGQVFVAAYKKRRQKLPEDYVDGAYYDPPPLKGTVV- 223

Query: 184 SKLPLTNQFR 190
            KLPLTNQ+R
Sbjct: 224 KKLPLTNQYR 232

The following BLAST results are available for this feature:

BLAST of Lsi04G004780.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PTR21_ARATH	8.5e-59	59.16	Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana GN=NPF2.13 PE=1 SV=1	[more]
PTR13_ARATH	1.0e-56	58.64	Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana GN=NPF2.12 PE=1 SV=2	[more]
PTR20_ARATH	3.2e-42	46.03	Putative protein NRT1/ PTR FAMILY 2.14 OS=Arabidopsis thaliana GN=NPF2.14 PE=5 S...	[more]
PTR53_ARATH	6.8e-40	44.97	Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana GN=NPF2.11 PE=1 SV=1	[more]
PTR44_ARATH	1.2e-39	44.92	Protein NRT1/ PTR FAMILY 2.10 OS=Arabidopsis thaliana GN=NPF2.10 PE=1 SV=3	[more]

BLAST of Lsi04G004780.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KTW3_CUCSA	2.8e-93	91.89	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G604170 PE=3 SV=1	[more]
U5G3E2_POPTR	1.1e-65	63.13	Proton-dependent oligopeptide transport family protein OS=Populus trichocarpa GN...	[more]
A0A022QN29_ERYGU	2.5e-65	66.32	Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a004515mg PE=4 SV=1	[more]
D7TNF6_VITVI	8.0e-64	62.69	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0026g01490 PE=4 SV=...	[more]
A0A067JBR4_JATCU	1.4e-63	66.32	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21712 PE=4 SV=1	[more]

BLAST of Lsi04G004780.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G69870.1	4.8e-60	59.16	nitrate transporter 1.7	[more]
AT1G27080.1	5.9e-58	58.64	nitrate transporter 1.6	[more]
AT1G69860.1	1.8e-43	46.03	Major facilitator superfamily protein	[more]
AT5G62680.1	3.8e-41	44.97	Major facilitator superfamily protein	[more]
AT3G47960.1	6.5e-41	44.92	Major facilitator superfamily protein	[more]

BLAST of Lsi04G004780.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659090965\|ref\|XP_008446297.1\|	7.4e-95	92.97	PREDICTED: LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 2.13-like [Cucumis melo...	[more]
gi\|449434837\|ref\|XP_004135202.1\|	4.0e-93	91.89	PREDICTED: protein NRT1/ PTR FAMILY 2.13-like [Cucumis sativus]	[more]
gi\|743924907\|ref\|XP_011006584.1\|	2.5e-66	63.64	PREDICTED: protein NRT1/ PTR FAMILY 2.13 [Populus euphratica]	[more]
gi\|566189009\|ref\|XP_006378165.1\|	1.6e-65	63.13	proton-dependent oligopeptide transport family protein [Populus trichocarpa]	[more]
gi\|604316501\|gb\|EYU28693.1\|	3.6e-65	66.32	hypothetical protein MIMGU_mgv1a004515mg [Erythranthe guttata]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Cellular Component**
Term	Definition
GO:0016020	membrane

Vocabulary: **Biological Process**
Term	Definition
GO:0006810	transport

Vocabulary: **Molecular Function**
Term	Definition
GO:0005215	transporter activity

Vocabulary: **INTERPRO**
Term	Definition
IPR020846	MFS_dom
IPR000109	POT_fam

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006810	transport
biological_process	GO:0006857	oligopeptide transport
cellular_component	GO:0016020	membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005215	transporter activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi04G004780	Lsi04G004780	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi04G004780.1.CDS.1	Lsi04G004780.1.CDS.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi04G004780.1	Lsi04G004780.1-protein	polypeptide

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000109	Proton-dependent oligopeptide transporter family	PANTHER	PTHR11654	OLIGOPEPTIDE TRANSPORTER-RELATED	coord: 4..189 score: 1.0E
IPR000109	Proton-dependent oligopeptide transporter family	PFAM	PF00854	PTR2	coord: 2..156 score: 3.5
IPR020846	Major facilitator superfamily domain	unknown	SSF103473	MFS general substrate transporter	coord: 33..130 score: 3.1
None	No IPR available	PANTHER	PTHR11654:SF20	PROTEIN NRT1/ PTR FAMILY 2.12-RELATED	coord: 4..189 score: 1.0E