Lsi01G020070.1 (mRNA) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi01G020070.1
Type	mRNA
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	S-adenosylmethionine: halide ion methyltransferase
Location	chr01 : 27372100 .. 27378891 (+)
Sequence length	840

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGTTGAGCTATGCTGATCTCTTTTTGCTATTTCTATATTTGGCAATTTAATTTCCTTGTACGAATTGATTTGCCGTAAATTTCTAAACGACCAAGTAGGTTCCAAATATGATCCTTGGCACTAGAAAATTGTCTTCAACACTTGGAGCTGCTCCCTTGCAAACCCATCGAAATTTGACGATGAAATTGAGGATGAACCAACAATATACCGAACATAACGCTTCGAAATCCAATCATCAATCGCGCGTCGATGAGTTTCAAAAAGTTGTGCGCAAAGACCATACTGGTAACACCAAATTTCATTTGATTCCACGTTTTATATATATCTGTTCTATGATGTTTTGGCCTTGAAGCTGCATCCAACTGATATATTACTGTTTTTTGGGATCAATAATGTATGCTGGATGAGCACATAATTTTAGAATTGAATTTTGGTGATTTAGGTTGCATTTCAAGAAGAAATACATTGATAGGTCTGAAATTCGATGGAATGATTGAGATTGATAATATCTGCTTCACTTCAGAACTATTTTTAATAGCTTAACCAGTCAATAATTATTGTAGAGTTTTTTGTGTATCAGCCTGGGCATCAGCTATTGAACCTTTTTACTTGACACATTTTTCTATAAAAATGTAGATTTTAGGTATAAAATTTTGATGGATGACTAACTTTTGTCTTCAATTTAGATGAATGCCAAAACCTGATTTCACTAATTCTGGATGTTTTATACGGTAATCAGGAGTTGCTGGTCAAATATGAAAATTAGTAGCTTCCTAGTTGGTTCTCATCATTTGGTTCCTCTCTCCTTATCTTCTTTTCTTTTTAGGCGCTCGACAATAAACTGTCTTGATGAACATTGAAAATATCTTTCTTGGACCACAAAAACATTAACGTGCGATTGTTGAAGAAAAAATCAGTTTTTAGGCTTTAATCATTTGTTACTGTGAAACTAATATTAGAGTAATGAAAGGTGTGCGAAATCGAGAGGTTTTCTAGGAAAACCAGTGCTATGGTGATTAATGTCCCATTGTTGTTAAGGTGCTCGGATGGTATGACATTCAACAACTTAGGATAAATTTTCTTTTCCCTCTGTCACCTCCGACAATGCCGACACGTCCCCCTCTTTCATATATGGAGTTAAGATTATCAACTCAATTTCAATTTATATTATGAAAATTTTCCTTTTAAAGTGCTAAAAAATTGTGTTTTGGGTGGTGGGAGAATAAATTATCCAAAACAATCATGACATTGAATAAAAATAAAATAAATATATGTATTTATAATTATATTATTAGTAAAAAAACATACTTTTTAAAATTAAAATACTAAGTTCGGGTTGGTTCAAGTTGTGTTGAGTTATTGTAGGTGAACTCATGAATCAACCCAACCCATAAAATTTTCATTTATTTGAACCCAACCCAATCCGACGATTGGGTTGGGTTGATCGGTTTTTTCGGGTCATCGGGTTTTTTGAACACTCCTACGCACAGCGTAATTCAAAATAACCTCCAGCCCACTATCACTTTCCATTATACCCCTTCTAACATACTTATTCACAATTGGAGCCTAACGTATAACTTCTAGATGGATATACCAAATTTAAATTTCATGGTTTGGTTCATGCAAATGTAGCATGTCTGATTTTGGAAGGGATTCTTTTGCATGTTTTGATGTTGTGTTGGACTGCATATTGTTTCTTTTTTCTTTGTTGTCTATTGTTACAATAATATGTCATCCAGTGTGCTCATGCTTTGAAGGGTTACTAATTAGGTGGTTGGGAGATATGCTGGGAGCAAGGTCTAACACCATGGGACTTGGGACAACCAACGCCTGTGATTAGCCATCTCTGTGCAACAGGAGCTCTTCCAAATGGTCGTGTACTTGTGCCTGGATGTGGAAGTGTACGTTATTTGATTTATATATAATTTTATAGCTATTTTTACTATATATATAAAAAAAAAAAAATTCATCTATAAAGATTGAAGTATGAAAATCAAGCCAGAAAATGGCGTTGACCAAAAGGGCAAATAACCAAGAAACTCTGCTTTACATTACATTTAAGAATTCCGACCAAGAATCAAGATAAGAAAAATGTAGAGAGGTTGAGAAGTAGAAACGGAAGTGAGGCATCGAAACATTCTAAGGTTTCTCTTTGAGAGATTAACAAAGGAAATGAGGAATATATTTTTGAGAGATTTCATAACATGGGAAGTTTAATTATCAACCTTCTATAGTTCTTCGTCTTCCTTTTATCTTCGAACCTATCTTGAAGATAATAGATGTGTTCCCTTTGCATGTAGTAGTTAGCTAGAATCTTACGATTTTTCTTTTTATAGAAAACATCAAAGTTCGTAAATATGACAGGGGTATGATGTTGTGCAAATGGCATGCTCTGAACGCTATGTTGTTGGATTGGACATATCAGACAATGCCATTCAAAAGGCAAAAGAGGTATAAGTATTATATTTCTGTACTTTTCTTCTTTACAGAGCCAAAATATTGGGAGAACAAAGGAAGTTTTAGGCCGTTTTGGGAATTTTCCACAAGAGAACTATTTGTGAATGTAATTGTTGGAACCTTAGCAGATATTCTTCTTATTTGTTCTATTAATTATCCAAGGTTTATTTTATTCTAACTTTTATCATATTTAAATGTAAATTATAGTATATATGTTTAGATTTAGTTAGTCACTTCCCTGGTAGATTCTGCTACCTTCTTTGTCTTCGATCTTTCCCCCAAGCCTCATGTAGACATAAATATATCTGAGTCATCTATCTTCATAAACATCTAATTCAATTTAAAAGATAGTTTGTCTATGTGCACATGGTTTCAGTGGGCTTGTAAACGCTCGGTTTAACAATGCTTGACATTTTTAGAGTGGTCGTAGTTTGCCTTGCTATGAAAGACACAGGAAAGTTGTAATGCCCATCACCCTTTCGCTTTCAACTTATAAGTGGAAAAAGTTTTAGAAAAAGTGAGCCCTAGTAAGAACTTAGCATGAGGTCTAAAGCATATACTATTAATGTGAAACAGAAAGAAACAAAGGGGCTTGGATTTAGCTCTTTTACTCTAACATCATGAAACTAACCGAACATGAGAGAATTGCATTATTTCATTTAATAAACATGTATATGGGAGCTCTAGTTTAGGAAATGGTATATTATAATAAAGGACTAATAAATAAACGGCCCATAATAGTTATGACCCACAAAACTAACAATATTATACAACTTATGAATCATCAAAGGTAAATTTCATGAATAATGTATTTTTTTTAAAAAAATTACAAAATTAAAGTCTTCTGTTTGAAAAAGAAATTAAAGTAGTGTTGAAATGAGCTGCACCGTTAAGACTCGCTGTTACATTTGAAAACAATAACTTATTTCTATTTTTCTTTAATGAGCTGCAGCTATCTTCCTCATCACCAAATATGAGATACTTTACCTTCTTAAAGGAAGATTTTTTCAGTTGGAGACCTAAGGAATTATTTGATCTGATTTTTGATTATACGTAAGTTGGCTTATGAAAAAATTATTTCCTATTAGAAGATTCCAACATGACCGCTATTGGAAACCATGCAGGTTTTTTTCTGCTATTGAACCTGTCATGAGGCTAGCTTGGGGACAACAAATTCAGAGGCTATTAAAGCCAAATGGAGAGCTTATAACACTAATGTTTCCTGTAATCATCTTTTCTTGAGACAAGTATTCTCTATATTTTTTCCTATAAGTTTTTATCATTTTAACAAGCCTAGAATGCAAAATTTCACCTCCTATTTCATTAGCAGTTCCCCTCCATGAATTGTTTTTTTTCCTATGACAATTCGTAATTTTTAGCGCACTTTTATCTTCTCTATCTTTTTCACTAATTCTTTTTCTTGGGCTTCCTTGTCATTTCACTATAAAAAATAATTTTCTTTAACAAAAACTGTAAAATGTCAGCACAATCTTGTCTTTAAAAGTAGAGTCATTTGATTGATCTTGTGCTGCGTAATATGTTTACGATTCTTTCCATTATTTCATGTTTGAAATACAATTTATTGGCTGGCAGATCGACGACCATAGTGGTGGACCACCTTACAAAGTTTCAGTTTCAGAGTAATTCTTAACCTTCACTTTGAATTTTTTCCTACTTGTTTAATTGGTCCTTTCTGTTCAAATATTTATCTAGATCAACTTGTTTTCCTTTTTCTGTTCCCTATAAAAATTGTAACTCCTTTATCTTTAAAAAAAATTAAGGTCATAAATATGAGGGATTAGATTTCTTATCTTTACTTGTTTTGTACCTTCTTCTAATAATCATTAAAATTGTGATTAGCTAGTTGAGTTGGCTGAAAGAAACGTCCTTGTCCTTGTCCATGGGATGATATTGAAGATGAGTGATGTATTTTCTCTTAAAGAAGAGTCTTAAGTCCTTGTCCATGGGATGATATTGAAGATGAATGATGTATTTTCTCTTAAAGAAGAGTCTTAAGTGGGTTTGTGGTGAGTGTTAGAGTGATATATGATATAACATTAAATTTACCTTCACTCATCAGCTTAAACTTTTGGTTCAATCGGTGATTTAACATGGTAGTAGAGTAGGAGGTCTAAGAGATCTTGTGTTCAAACTCCCTGCAATGGCTCATTTTCTCCCTAATTAATATTGATTCCACTTGTTAGTCTTCTACATATTTCAAGTCCACAAGTAAGGAGAGTGTTAGAGTGTTGATATAATATTAAATTTACCTTCACCCATCAACTTAAGTTTTTTTTGTCAATCTCATTAGGTGGAAAGGATTGCCCTTTTCTTATCTCGTGTTCTGCCTTGGCAATAATCAACATCTCTACTCCTGTTAGGATCCTAGTTGGTGGAATAGTTTAAAGCCAAGTTGGACAAGTAGTGAAACCTCCTTGTCTCATTAGCGGTAGATTGGCTTTGGCTCAGCCTGTTCTATTCCCAACAGGATGCTCATTTACTATTTTCCAAACTTAGGGCATCTATTAAAGTTATTTACTCAATGGCGATATATGAGAGATTTCATTTGAATGGAGGGGTTTACTGAAGCCATTTGGTGAAATGGCATTGTACTTATCTCTCCATTCACTATCGAGTCTTGGGTTGGTATGAGAGAACTAGTTAAGACATTATATTTTCAACTAAAGGTCAGATGTTCAAATTTTAACTTTCACATGTTAAATTAAAAAAAGAATAAAGGTAATCAATCATCTTTTTTCAACATTGAGTTCTAATCTTAGGCGGACTCTAAACATGACTATAAATAAGTAAAAAAGTTTGGAAACTTTATAAGGCTATAGCAAATTTTGGTTGCCAAAATGCAACAAACACAGTGAAAATTAAGAATACCCGACTCATTTGGAAATAATTATTTTAAGCGAAGTAATACTACTTCAACATTGAGTTTTGTTATTATTTATTTATTTATTTACATTATTATTAATCGAAGCACTTTGTTGAAGTAGTAGAGACCATTAGGTAGCACATTTAATCATTTTTCTCAAGCCTTGATTTTGAAAAATACAATCTGCTGTAGATGAAGGGCATTTTCATATACCTGCTTGATATATTTTGTTTATTATTGTCGTTGTATCCTCTAGCTCTATTTGGAATTAGGGAAGAGAATATGTGTAAACCACTATTTTGTGGCCTTAGAGTATCCATATATGTGTTAGGATACTTCCTAACAAGAACAAGATCTGATATTTAGTACCTAACAATACGTTAAAGAATCTCAGTGGTTCAATTTTGTGGTCTAATGCGTCAACAACTAGAATTCGGAAGATTTTGTTGGAGAGAGATGGGATGTCTTTCAAGGATTTTATAGGAACTTCTTGCATATGTTGAGCTTTGTTGCATCAACTTGGCACGTTCTACATTTTCGTTTTGTTGTTACTCATTATCAGTGCATTTCACTTGAAGCGTGTTTTTGTAATCTTCATTTTCATTTTGGTTAGGAGGGTATCTTATCTTCCCTTCCCTTCTGTCATGTATATCCGATTCCTTTTGGAATCGTATTATCTTTTTTGTTTCCTTGAAAAAAGATTACTTTTTGTATCTTCGCATATATATGTGTATATATATCTCGTCCTTCCATAAACTTGGCTCATGATTACATCGTTTATTGATAGTTATGAGGACGTACTACATCCATTGGGCTTTAAGGCTGTACTGATCAAGGACAATGAACTCGCCATCCCACCTCGCAAGGTATATATAGCAACTTTTTCGGTACGCTACAGAAGTTTCAATTGTCTGTTAAAACTCGATTTTGTTGTCAACAATTAATTGGCAACAGCAAAGAGAGATCTGTTCTCTCCAAGAACACTCAAAATAAGAGTTCCAAACAAAATAGCAGAAGCTTACCCCCTCCTTCCTTCGAACACGCCCTTAAAAGGCTACTCCTGTACAGAAAAAAATGCTCACCCAAAAGATTATAATACACTGGTGCTCATTCGCCTCCCCTATTCTCTCTCTTTTTTCCTACGTGTTAGAACGTAGTGACTTGGGTCTATCATCAAGAAAGTCATGCAACCACTATTTGAGCTACTGTAAATTGTCAATTAGAGCATAACACAACTAGTTAAGGTATTGTGGCCTTTATCAAATTCTCGCCACTCAAAAGTAAATGGACTTTTATAACTTATTTTTTAAAAATGCAATGTTATCCATGTTGTGTAATTGCTGAATGTTCAGTATGATTGAAGTAAATTTTATGACAGGGACGAGAGATGATTGGAAGGTGGAAGAGGAGTTTAGCTCAAGCTTCACTCTAACAAGTTTATAGCCAC

mRNA sequence

ATGAGAAAATTGTCTTCAACACTTGGAGCTGCTCCCTTGCAAACCCATCGAAATTTGACGATGAAATTGAGGATGAACCAACAATATACCGAACATAACGCTTCGAAATCCAATCATCAATCGCGCGTCGATGAGTTTCAAAAAGTTGTGCGCAAAGACCATACTGGTGGTTGGGAGATATGCTGGGAGCAAGGTCTAACACCATGGGACTTGGGACAACCAACGCCTGTGATTAGCCATCTCTGTGCAACAGGAGCTCTTCCAAATGGTCGTGTACTTGTGCCTGGATGTGGAAGTGGGTATGATGTTGTGCAAATGGCATGCTCTGAACGCTATGTTGTTGGATTGGACATATCAGACAATGCCATTCAAAAGGCAAAAGAGAGCCAAAATATTGGGAGAACAAAGGAACTATCTTCCTCATCACCAAATATGAGATACTTTACCTTCTTAAAGGAAGATTTTTTCAGTTGGAGACCTAAGGAATTATTTGATCTGATTTTTGATTATACATTCCAACATGACCGCTATTGGAAACCATGCAGGTTTTTTTCTGCTATTGAACCTGTCATGAGGCTAGCTTGGGGACAACAAATTCAGAGGCTATTAAAGCCAAATGGAGAGCTTATAACACTAATGTTTCCTATCGACGACCATAGTGGTGGACCACCTTACAAAGTTTCAGTTTCAGATTATGAGGACGTACTACATCCATTGGGCTTTAAGGCTGTACTGATCAAGGACAATGAACTCGCCATCCCACCTCGCAAGGGACGAGAGATGATTGGAAGGTGGAAGAGGAGTTTAGCTCAAGCTTCACTCTAACAAGTTTATAGCCAC

Coding sequence (CDS)

ATGAGAAAATTGTCTTCAACACTTGGAGCTGCTCCCTTGCAAACCCATCGAAATTTGACGATGAAATTGAGGATGAACCAACAATATACCGAACATAACGCTTCGAAATCCAATCATCAATCGCGCGTCGATGAGTTTCAAAAAGTTGTGCGCAAAGACCATACTGGTGGTTGGGAGATATGCTGGGAGCAAGGTCTAACACCATGGGACTTGGGACAACCAACGCCTGTGATTAGCCATCTCTGTGCAACAGGAGCTCTTCCAAATGGTCGTGTACTTGTGCCTGGATGTGGAAGTGGGTATGATGTTGTGCAAATGGCATGCTCTGAACGCTATGTTGTTGGATTGGACATATCAGACAATGCCATTCAAAAGGCAAAAGAGAGCCAAAATATTGGGAGAACAAAGGAACTATCTTCCTCATCACCAAATATGAGATACTTTACCTTCTTAAAGGAAGATTTTTTCAGTTGGAGACCTAAGGAATTATTTGATCTGATTTTTGATTATACATTCCAACATGACCGCTATTGGAAACCATGCAGGTTTTTTTCTGCTATTGAACCTGTCATGAGGCTAGCTTGGGGACAACAAATTCAGAGGCTATTAAAGCCAAATGGAGAGCTTATAACACTAATGTTTCCTATCGACGACCATAGTGGTGGACCACCTTACAAAGTTTCAGTTTCAGATTATGAGGACGTACTACATCCATTGGGCTTTAAGGCTGTACTGATCAAGGACAATGAACTCGCCATCCCACCTCGCAAGGGACGAGAGATGATTGGAAGGTGGAAGAGGAGTTTAGCTCAAGCTTCACTCTAA

Protein sequence

MRKLSSTLGAAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEICWEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKDNELAIPPRKGREMIGRWKRSLAQASL

Homology

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BLAST of Lsi01G020070.1 vs. Swiss-Prot
Match: HOL3_ARATH (Probable thiol methyltransferase 2 OS=Arabidopsis thaliana GN=HOL3 PE=1 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 6.1e-74
Identity = 138/237 (58.23%), Postives = 173/237 (73.00%), Query Frame = 1


Query: 33  NASKSNH-QSRVDEFQKVVRKDHTGGWEICWEQGLTPWDLGQPTPVISHLCATGALPNGR 92
           NA K+   QS  D F +++ ++ +GGWE  WE G TPWDLG+PTPVI+HL  TG+LPNGR
Sbjct: 3   NAGKATSLQSSRDLFHRLMSENSSGGWEKSWEAGATPWDLGKPTPVIAHLVETGSLPNGR 62

Query: 93  VLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKESQNIGRTKELSSSSPNMRYFTFL 152
            LVPGCG+GYDVV MA  +R+VVGLDIS  A++++        TK+ S+  PN +YF+FL
Sbjct: 63  ALVPGCGTGYDVVAMASPDRHVVGLDISKTAVERS--------TKKFSTL-PNAKYFSFL 122

Query: 153 KEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEPVMRLAWGQQIQRLLKPNGELIT 212
            EDFF+W P E FDLIFDYTF           F A EP +R  W Q++++LLKP GELIT
Sbjct: 123 SEDFFTWEPAEKFDLIFDYTF-----------FCAFEPGVRPLWAQRMEKLLKPGGELIT 182

Query: 213 LMFPIDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKDNELAIPPRKGREMIGRWKRS 269
           LMFPID+ SGGPPY+VSVS+YE VL PLGF+A+ I DNELA+ PRKG E +GRWK+S
Sbjct: 183 LMFPIDERSGGPPYEVSVSEYEKVLIPLGFEAISIVDNELAVGPRKGMEKLGRWKKS 219

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BLAST of Lsi01G020070.1 vs. Swiss-Prot
Match: TMT1_BRAOL (Thiocyanate methyltransferase 1 OS=Brassica oleracea GN=TMT1 PE=1 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 5.9e-69
Identity = 125/212 (58.96%), Postives = 153/212 (72.17%), Query Frame = 1


Query: 56  GGWEICWEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVG 115
           GGWE CWE G+TPWD G+ TP++ HL  + +LP GR LVPGCG G+DVV MA  ER+VVG
Sbjct: 33  GGWEKCWEDGITPWDQGRATPLVVHLVDSSSLPLGRALVPGCGGGHDVVAMASPERFVVG 92

Query: 116 LDISDNAIQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHD 175
           LDIS++A++KA E+           SSP  +YFTF+KEDFF+WRP ELFDLIFDY     
Sbjct: 93  LDISESALEKAAETYG---------SSPKAKYFTFVKEDFFTWRPNELFDLIFDYVV--- 152

Query: 176 RYWKPCRFFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPIDDHSGGPPYKVSVSDYEDV 235
                   F AIEP MR AW + +  LLKP+GELITLM+PI DH GGPPYKV+VS YEDV
Sbjct: 153 --------FCAIEPEMRPAWAKSMYELLKPDGELITLMYPITDHDGGPPYKVAVSTYEDV 212

Query: 236 LHPLGFKAVLIKDNELAIPPRKGREMIGRWKR 268
           L P+GFKAV I++N  +I  RKG+E +GRWK+
Sbjct: 213 LVPVGFKAVSIEENPYSIATRKGKEKLGRWKK 224

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BLAST of Lsi01G020070.1 vs. Swiss-Prot
Match: HOL2_ARATH (Probable thiol methyltransferase 1 OS=Arabidopsis thaliana GN=HOL2 PE=1 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.5e-67
Identity = 123/212 (58.02%), Postives = 150/212 (70.75%), Query Frame = 1


Query: 56  GGWEICWEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVG 115
           GGW+ CWE G+TPWD G+ TP+I HL  + ALP GR LVPGCG G+DVV MA  ER+VVG
Sbjct: 34  GGWDKCWEDGVTPWDQGRATPLILHLLDSSALPLGRTLVPGCGGGHDVVAMASPERFVVG 93

Query: 116 LDISDNAIQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHD 175
           LDISD A+ KA E+           SSP   YF+F+KED F+WRP ELFDLIFDY F   
Sbjct: 94  LDISDKALNKANETYG---------SSPKAEYFSFVKEDVFTWRPNELFDLIFDYVF--- 153

Query: 176 RYWKPCRFFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPIDDHSGGPPYKVSVSDYEDV 235
                   F AIEP MR AWG+ +  LLKP+GELITLM+P+ DH GG PYKV++S YEDV
Sbjct: 154 --------FCAIEPEMRPAWGKSMHELLKPDGELITLMYPMTDHEGGAPYKVALSSYEDV 213

Query: 236 LHPLGFKAVLIKDNELAIPPRKGREMIGRWKR 268
           L P+GFKAV +++N  +IP RKG+E + RWK+
Sbjct: 214 LVPVGFKAVSVEENPDSIPTRKGKEKLARWKK 225

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BLAST of Lsi01G020070.1 vs. Swiss-Prot
Match: TMT2_BRAOL (Probable thiocyanate methyltransferase 2 OS=Brassica oleracea GN=TMT2 PE=2 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.5e-67
Identity = 129/240 (53.75%), Postives = 164/240 (68.33%), Query Frame = 1


Query: 31  EHNASKSNHQSRV--DEFQKVVRKD-HTGGWEICWEQGLTPWDLGQPTPVISHLCATGAL 90
           + N+  SN ++ +  ++  K + K    GGWE CWE G+TPWD G+ TP++ HL  + +L
Sbjct: 5   QQNSGNSNGENIIPPEDVAKFLPKTVDEGGWEKCWEDGVTPWDQGRATPLVVHLVESSSL 64

Query: 91  PNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKESQNIGRTKELSSSSPNMRY 150
           P GR LVPGCG G+DVV MA  ERYVVGLDIS++A++KA E+           SSP  +Y
Sbjct: 65  PLGRGLVPGCGGGHDVVAMASPERYVVGLDISESALEKAAETYG---------SSPKAKY 124

Query: 151 FTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEPVMRLAWGQQIQRLLKPNG 210
           FTF+KEDFF+WRP ELFDLIFDY             F AIEP  R AW + +  LLKP+G
Sbjct: 125 FTFVKEDFFTWRPNELFDLIFDYVV-----------FCAIEPETRPAWAKAMYELLKPDG 184

Query: 211 ELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKDNELAIPPRKGREMIGRWKR 268
           ELITLM+PI DH GGPPYKV+VS YEDVL P+GFKAV I++N  +I  RKG+E + RWK+
Sbjct: 185 ELITLMYPITDHDGGPPYKVAVSTYEDVLVPVGFKAVSIEENPYSIATRKGKEKLARWKK 224

[Close]

BLAST of Lsi01G020070.1 vs. Swiss-Prot
Match: HOL1_ARATH (Thiocyanate methyltransferase 1 OS=Arabidopsis thaliana GN=HOL1 PE=1 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 7.0e-62
Identity = 122/231 (52.81%), Postives = 154/231 (66.67%), Query Frame = 1


Query: 31  EHNASKSNHQSRVDEFQKVVRKDHT----GGWEICWEQGLTPWDLGQPTPVISHLCATGA 90
           + N+ +SN  + +   ++V    H     GGWE CWE+ +TPWD G+ TP+I HL  T +
Sbjct: 5   QQNSDQSNGGNVIPTPEEVATFLHKTVEEGGWEKCWEEEITPWDQGRATPLIVHLVDTSS 64

Query: 91  LPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKESQNIGRTKELSSSSPNMR 150
           LP GR LVPGCG G+DVV MA  ER+VVGLDIS++A+ KA E+           SSP   
Sbjct: 65  LPLGRALVPGCGGGHDVVAMASPERFVVGLDISESALAKANETY---------GSSPKAE 124

Query: 151 YFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEPVMRLAWGQQIQRLLKPN 210
           YF+F+KED F+WRP ELFDLIFDY            FF AIEP MR AW + +  LLKP+
Sbjct: 125 YFSFVKEDVFTWRPTELFDLIFDYV-----------FFCAIEPEMRPAWAKSMYELLKPD 184

Query: 211 GELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKDNELAIPPRK 258
           GELITLM+PI DH GGPPYKV VS +E+VL P+GFKAV +++N  AIP R+
Sbjct: 185 GELITLMYPITDHVGGPPYKVDVSTFEEVLVPIGFKAVSVEENPHAIPTRQ 215

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BLAST of Lsi01G020070.1 vs. TrEMBL
Match: A0A0A0KYY5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G439050 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.0e-120
Identity = 222/273 (81.32%), Postives = 231/273 (84.62%), Query Frame = 1


Query: 3   KLSSTLGAAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEICW 62
           K  S L  APLQTHR LT+KLRMNQ  T+HNASK NHQSRV EFQKV+  DH+GGW+ICW
Sbjct: 7   KFCSILSRAPLQTHRALTLKLRMNQNSTQHNASKFNHQSRVHEFQKVMGTDHSGGWDICW 66

Query: 63  EQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNA 122
           EQGLTPWDLGQPTP+I HLCATGALPNGRVLVPGCGSGYDVVQMAC+ERYVVGLDISD A
Sbjct: 67  EQGLTPWDLGQPTPLIGHLCATGALPNGRVLVPGCGSGYDVVQMACAERYVVGLDISDKA 126

Query: 123 IQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCR 182
           IQKA          ELSSSSPNMR+ TFLKEDFFSWRPKELFDLIFDYT           
Sbjct: 127 IQKA---------IELSSSSPNMRHCTFLKEDFFSWRPKELFDLIFDYT----------- 186

Query: 183 FFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPI-DDHSGGPPYKVSVSDYEDVLHPLGF 242
           FF AIEPVMR AWGQQIQRLLKPNGELITLMFPI DDH GGPPYKVSVSDYEDVLHPLGF
Sbjct: 187 FFCAIEPVMRQAWGQQIQRLLKPNGELITLMFPIDDDHIGGPPYKVSVSDYEDVLHPLGF 246

Query: 243 KAVLIKDNELAIPPRKGREMIGRWKRSLAQASL 275
           KAVLI DNELA+PPRKGREMIGRWKRSLAQ SL
Sbjct: 247 KAVLINDNELAVPPRKGREMIGRWKRSLAQPSL 259

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BLAST of Lsi01G020070.1 vs. TrEMBL
Match: B9IJ56_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s03980g PE=4 SV=2)

HSP 1 Score: 313.9 bits (803), Expect = 1.9e-82
Identity = 154/232 (66.38%), Postives = 172/232 (74.14%), Query Frame = 1


Query: 43  VDEFQKVVRKDHTGGWEICWEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYD 102
           V + QK+V  + +GGWE CWEQGLTPWDLG+PTP+I HL  TGALP GR LVPGCGSGYD
Sbjct: 62  VHQMQKLVTTETSGGWENCWEQGLTPWDLGRPTPIILHLHQTGALPKGRALVPGCGSGYD 121

Query: 103 VVQMACSERYVVGLDISDNAIQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKE 162
           VV MACSERYVVGLD+S  AI+KA          ELSSS PN  YFTFLK DFF+W P E
Sbjct: 122 VVAMACSERYVVGLDVSHTAIEKA---------IELSSSLPNSSYFTFLKADFFTWHPPE 181

Query: 163 LFDLIFDYTFQHDRYWKPCRFFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPIDDHSGG 222
           LFDLIFDYT           FF AIEP MR  W  ++Q +LKP+GELITLM+PI DH GG
Sbjct: 182 LFDLIFDYT-----------FFCAIEPGMRSRWACKVQEMLKPDGELITLMYPISDHVGG 241

Query: 223 PPYKVSVSDYEDVLHPLGFKAVLIKDNELAIPPRKGREMIGRWKRSLAQASL 275
           PPYKVSVSDYE+VLHP+GFKAV I DNELAI  RKGRE  GRW+R   Q+ L
Sbjct: 242 PPYKVSVSDYEEVLHPMGFKAVTIVDNELAIEARKGREKFGRWRRDTTQSIL 273

[Close]

BLAST of Lsi01G020070.1 vs. TrEMBL
Match: A0A061EXJ7_THECC (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_021543 PE=4 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 8.0e-81
Identity = 157/274 (57.30%), Postives = 197/274 (71.90%), Query Frame = 1


Query: 1   MRKLSSTLGAAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEI 60
           +R  S+TLG   +  +         N+  T  +  +SN   RV + Q++V  + +GGWE 
Sbjct: 17  LRVPSTTLGTIRVMNN---------NRHRTADSRVESN--PRVHKLQQLVNTNASGGWEE 76

Query: 61  CWEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISD 120
            W+QG+TPWDLG+PTPVISHL  TG+LP GRVLVPGCG+GYDVV+MAC  RYVVGLDIS+
Sbjct: 77  SWKQGVTPWDLGRPTPVISHLHHTGSLPMGRVLVPGCGTGYDVVEMACLGRYVVGLDISE 136

Query: 121 NAIQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKP 180
            AI+KAK+         +SS+ PN  YFTF+K DFF+W P +LFDLIFDYTF        
Sbjct: 137 EAIKKAKQ---------MSSTLPNADYFTFIKADFFTWHPTDLFDLIFDYTF-------- 196

Query: 181 CRFFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLG 240
              F AIEP MR AW QQIQ  LKP+GELITLMFP+DDH+GGPPYKVS++DY++VLHP+G
Sbjct: 197 ---FCAIEPDMRSAWAQQIQNFLKPDGELITLMFPMDDHAGGPPYKVSIADYKEVLHPMG 256

Query: 241 FKAVLIKDNELAIPPRKGREMIGRWKRSLAQASL 275
           F+A+ I DN+ AI  RKGRE +GRWKR+L Q+SL
Sbjct: 257 FEAISIVDNKWAIEVRKGREKLGRWKRALGQSSL 259

[Close]

BLAST of Lsi01G020070.1 vs. TrEMBL
Match: A0A067K092_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19270 PE=4 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 2.3e-80
Identity = 161/260 (61.92%), Postives = 184/260 (70.77%), Query Frame = 1


Query: 15  THRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEICWEQGLTPWDLGQP 74
           T +N+  K R  Q Y   +  K N Q  V + Q+++  D +GGWE CWEQGLTPWDLGQP
Sbjct: 36  TSQNMDNK-RKEQLYRNESNVKFNPQ--VHQIQRLLETDSSGGWEKCWEQGLTPWDLGQP 95

Query: 75  TPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKESQNIGR 134
           TPV+ HL  TGALP GR LVPGCGSG+DV  +A  ERYVVGLD+S  AI+KA+E      
Sbjct: 96  TPVLLHLHRTGALPMGRALVPGCGSGHDVFAIASPERYVVGLDVSGQAIKKAEE------ 155

Query: 135 TKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEPVMRLA 194
              LSSSSPN   FTFLK DFF+W P ELFDLI DYTF           F AIEP MR  
Sbjct: 156 ---LSSSSPNANCFTFLKADFFTWHPPELFDLIIDYTF-----------FCAIEPEMRAR 215

Query: 195 WGQQIQRLLKPNGELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKDNELAIP 254
           W  +IQ LLKP+GELITL+FPIDDH GGPPYKVSVSDYE+VLHPLGFKAV I DN+LAI 
Sbjct: 216 WALKIQDLLKPDGELITLIFPIDDHVGGPPYKVSVSDYEEVLHPLGFKAVSIVDNDLAIK 272

Query: 255 PRKGREMIGRWKRSLAQASL 275
            RKGRE +GRWKRS  ++ L
Sbjct: 276 ARKGREKLGRWKRSSTRSLL 272

[Close]

BLAST of Lsi01G020070.1 vs. TrEMBL
Match: A0A0B0MY55_GOSAR (Putative thiol methyltransferase 2-like protein OS=Gossypium arboreum GN=F383_05001 PE=4 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.0e-79
Identity = 149/249 (59.84%), Postives = 185/249 (74.30%), Query Frame = 1


Query: 26  NQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEICWEQGLTPWDLGQPTPVISHLCATG 85
           N+  T  ++ ++N   R+ + Q++V+ D + GWE  W+QG+TPWDLG+PTPVI HL  +G
Sbjct: 31  NRSRTTDSSVQTN--PRIQKLQQIVKTDASAGWEESWKQGVTPWDLGRPTPVILHLHHSG 90

Query: 86  ALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKESQNIGRTKELSSSSPNM 145
           +LP GRVLVPGCG+GYDVV MAC  RYVVGLDIS+ AI+KAK+         +SSS PN 
Sbjct: 91  SLPMGRVLVPGCGTGYDVVAMACPGRYVVGLDISEEAIKKAKQ---------MSSSLPNA 150

Query: 146 RYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEPVMRLAWGQQIQRLLKP 205
             FTF++ +FFSWRP +LFDLIFDYTF           F AI P MRLAW QQIQ  LKP
Sbjct: 151 DNFTFIEANFFSWRPTDLFDLIFDYTF-----------FCAILPEMRLAWAQQIQNFLKP 210

Query: 206 NGELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKDNELAIPPRKGREMIGRW 265
           +GEL+TLMFP+DDH+GGPP+KVS+ DYE+VLHP+GFKAV I DNELAI  RKGRE +GRW
Sbjct: 211 DGELVTLMFPMDDHAGGPPFKVSIEDYEEVLHPMGFKAVSIVDNELAIEARKGREKLGRW 257

Query: 266 KRSLAQASL 275
           KR +  +SL
Sbjct: 271 KRGVGHSSL 257

[Close]

BLAST of Lsi01G020070.1 vs. TAIR10
Match: AT2G43940.1 (AT2G43940.1 S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 278.9 bits (712), Expect = 3.4e-75
Identity = 138/237 (58.23%), Postives = 173/237 (73.00%), Query Frame = 1


Query: 33  NASKSNH-QSRVDEFQKVVRKDHTGGWEICWEQGLTPWDLGQPTPVISHLCATGALPNGR 92
           NA K+   QS  D F +++ ++ +GGWE  WE G TPWDLG+PTPVI+HL  TG+LPNGR
Sbjct: 3   NAGKATSLQSSRDLFHRLMSENSSGGWEKSWEAGATPWDLGKPTPVIAHLVETGSLPNGR 62

Query: 93  VLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKESQNIGRTKELSSSSPNMRYFTFL 152
            LVPGCG+GYDVV MA  +R+VVGLDIS  A++++        TK+ S+  PN +YF+FL
Sbjct: 63  ALVPGCGTGYDVVAMASPDRHVVGLDISKTAVERS--------TKKFSTL-PNAKYFSFL 122

Query: 153 KEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEPVMRLAWGQQIQRLLKPNGELIT 212
            EDFF+W P E FDLIFDYTF           F A EP +R  W Q++++LLKP GELIT
Sbjct: 123 SEDFFTWEPAEKFDLIFDYTF-----------FCAFEPGVRPLWAQRMEKLLKPGGELIT 182

Query: 213 LMFPIDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKDNELAIPPRKGREMIGRWKRS 269
           LMFPID+ SGGPPY+VSVS+YE VL PLGF+A+ I DNELA+ PRKG E +GRWK+S
Sbjct: 183 LMFPIDERSGGPPYEVSVSEYEKVLIPLGFEAISIVDNELAVGPRKGMEKLGRWKKS 219

[Close]

BLAST of Lsi01G020070.1 vs. TAIR10
Match: AT2G43920.1 (AT2G43920.1 S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 257.7 bits (657), Expect = 8.2e-69
Identity = 123/212 (58.02%), Postives = 150/212 (70.75%), Query Frame = 1


Query: 56  GGWEICWEQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVG 115
           GGW+ CWE G+TPWD G+ TP+I HL  + ALP GR LVPGCG G+DVV MA  ER+VVG
Sbjct: 34  GGWDKCWEDGVTPWDQGRATPLILHLLDSSALPLGRTLVPGCGGGHDVVAMASPERFVVG 93

Query: 116 LDISDNAIQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHD 175
           LDISD A+ KA E+           SSP   YF+F+KED F+WRP ELFDLIFDY F   
Sbjct: 94  LDISDKALNKANETYG---------SSPKAEYFSFVKEDVFTWRPNELFDLIFDYVF--- 153

Query: 176 RYWKPCRFFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPIDDHSGGPPYKVSVSDYEDV 235
                   F AIEP MR AWG+ +  LLKP+GELITLM+P+ DH GG PYKV++S YEDV
Sbjct: 154 --------FCAIEPEMRPAWGKSMHELLKPDGELITLMYPMTDHEGGAPYKVALSSYEDV 213

Query: 236 LHPLGFKAVLIKDNELAIPPRKGREMIGRWKR 268
           L P+GFKAV +++N  +IP RKG+E + RWK+
Sbjct: 214 LVPVGFKAVSVEENPDSIPTRKGKEKLARWKK 225

[Close]

BLAST of Lsi01G020070.1 vs. TAIR10
Match: AT2G43910.2 (AT2G43910.2 HARMLESS TO OZONE LAYER 1)

HSP 1 Score: 238.8 bits (608), Expect = 3.9e-63
Identity = 122/231 (52.81%), Postives = 154/231 (66.67%), Query Frame = 1


Query: 31  EHNASKSNHQSRVDEFQKVVRKDHT----GGWEICWEQGLTPWDLGQPTPVISHLCATGA 90
           + N+ +SN  + +   ++V    H     GGWE CWE+ +TPWD G+ TP+I HL  T +
Sbjct: 5   QQNSDQSNGGNVIPTPEEVATFLHKTVEEGGWEKCWEEEITPWDQGRATPLIVHLVDTSS 64

Query: 91  LPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKESQNIGRTKELSSSSPNMR 150
           LP GR LVPGCG G+DVV MA  ER+VVGLDIS++A+ KA E+           SSP   
Sbjct: 65  LPLGRALVPGCGGGHDVVAMASPERFVVGLDISESALAKANETY---------GSSPKAE 124

Query: 151 YFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEPVMRLAWGQQIQRLLKPN 210
           YF+F+KED F+WRP ELFDLIFDY            FF AIEP MR AW + +  LLKP+
Sbjct: 125 YFSFVKEDVFTWRPTELFDLIFDYV-----------FFCAIEPEMRPAWAKSMYELLKPD 184

Query: 211 GELITLMFPIDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKDNELAIPPRK 258
           GELITLM+PI DH GGPPYKV VS +E+VL P+GFKAV +++N  AIP R+
Sbjct: 185 GELITLMYPITDHVGGPPYKVDVSTFEEVLVPIGFKAVSVEENPHAIPTRQ 215

[Close]

BLAST of Lsi01G020070.1 vs. NCBI nr
Match: gi|449453195|ref|XP_004144344.1| (PREDICTED: probable thiol methyltransferase 2 [Cucumis sativus])

HSP 1 Score: 441.0 bits (1133), Expect = 1.5e-120
Identity = 222/273 (81.32%), Postives = 231/273 (84.62%), Query Frame = 1


Query: 3   KLSSTLGAAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEICW 62
           K  S L  APLQTHR LT+KLRMNQ  T+HNASK NHQSRV EFQKV+  DH+GGW+ICW
Sbjct: 7   KFCSILSRAPLQTHRALTLKLRMNQNSTQHNASKFNHQSRVHEFQKVMGTDHSGGWDICW 66

Query: 63  EQGLTPWDLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNA 122
           EQGLTPWDLGQPTP+I HLCATGALPNGRVLVPGCGSGYDVVQMAC+ERYVVGLDISD A
Sbjct: 67  EQGLTPWDLGQPTPLIGHLCATGALPNGRVLVPGCGSGYDVVQMACAERYVVGLDISDKA 126

Query: 123 IQKAKESQNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCR 182
           IQKA          ELSSSSPNMR+ TFLKEDFFSWRPKELFDLIFDYT           
Sbjct: 127 IQKA---------IELSSSSPNMRHCTFLKEDFFSWRPKELFDLIFDYT----------- 186

Query: 183 FFSAIEPVMRLAWGQQIQRLLKPNGELITLMFPI-DDHSGGPPYKVSVSDYEDVLHPLGF 242
           FF AIEPVMR AWGQQIQRLLKPNGELITLMFPI DDH GGPPYKVSVSDYEDVLHPLGF
Sbjct: 187 FFCAIEPVMRQAWGQQIQRLLKPNGELITLMFPIDDDHIGGPPYKVSVSDYEDVLHPLGF 246

Query: 243 KAVLIKDNELAIPPRKGREMIGRWKRSLAQASL 275
           KAVLI DNELA+PPRKGREMIGRWKRSLAQ SL
Sbjct: 247 KAVLINDNELAVPPRKGREMIGRWKRSLAQPSL 259

[Close]

BLAST of Lsi01G020070.1 vs. NCBI nr
Match: gi|659069382|ref|XP_008449545.1| (PREDICTED: probable thiol methyltransferase 2 isoform X1 [Cucumis melo])

HSP 1 Score: 440.7 bits (1132), Expect = 1.9e-120
Identity = 221/266 (83.08%), Postives = 230/266 (86.47%), Query Frame = 1


Query: 10  AAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEICWEQGLTPW 69
           AAPLQTHR LT+KLRMNQ  TEH ASK NHQSRV EFQKV+  DH+GGW+ICWE GLTPW
Sbjct: 15  AAPLQTHRALTLKLRMNQNSTEHKASKFNHQSRVHEFQKVMGTDHSGGWDICWEHGLTPW 74

Query: 70  DLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKES 129
           DLGQPTP+ISHLCATGALPNGRVLVPGCGSGYDVVQMAC+ERYVVGLDISDNAIQKA E 
Sbjct: 75  DLGQPTPLISHLCATGALPNGRVLVPGCGSGYDVVQMACAERYVVGLDISDNAIQKAIE- 134

Query: 130 QNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEP 189
                   LSSSSPNMR+ TFL+EDFFSWRPKELFDLIFDYT           FF AIEP
Sbjct: 135 --------LSSSSPNMRHCTFLREDFFSWRPKELFDLIFDYT-----------FFCAIEP 194

Query: 190 VMRLAWGQQIQRLLKPNGELITLMFPI-DDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKD 249
           VMR  WGQQIQRLLKPNGELITLMFPI DDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKD
Sbjct: 195 VMRQVWGQQIQRLLKPNGELITLMFPIDDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKD 254

Query: 250 NELAIPPRKGREMIGRWKRSLAQASL 275
           NELAIPPRKGREMIGRWKRSL+Q SL
Sbjct: 255 NELAIPPRKGREMIGRWKRSLSQPSL 260

[Close]

BLAST of Lsi01G020070.1 vs. NCBI nr
Match: gi|659069384|ref|XP_008449554.1| (PREDICTED: probable thiol methyltransferase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 410.2 bits (1053), Expect = 2.8e-111
Identity = 206/249 (82.73%), Postives = 214/249 (85.94%), Query Frame = 1


Query: 10  AAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEICWEQGLTPW 69
           AAPLQTHR LT+KLRMNQ  TEH ASK NHQSRV EFQKV+  DH+GGW+ICWE GLTPW
Sbjct: 15  AAPLQTHRALTLKLRMNQNSTEHKASKFNHQSRVHEFQKVMGTDHSGGWDICWEHGLTPW 74

Query: 70  DLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKES 129
           DLGQPTP+ISHLCATGALPNGRVLVPGCGSGYDVVQMAC+ERYVVGLDISDNAIQKA   
Sbjct: 75  DLGQPTPLISHLCATGALPNGRVLVPGCGSGYDVVQMACAERYVVGLDISDNAIQKA--- 134

Query: 130 QNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEP 189
                  ELSSSSPNMR+ TFL+EDFFSWRPKELFDLIFDYT           FF AIEP
Sbjct: 135 ------IELSSSSPNMRHCTFLREDFFSWRPKELFDLIFDYT-----------FFCAIEP 194

Query: 190 VMRLAWGQQIQRLLKPNGELITLMFPI-DDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKD 249
           VMR  WGQQIQRLLKPNGELITLMFPI DDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKD
Sbjct: 195 VMRQVWGQQIQRLLKPNGELITLMFPIDDDHSGGPPYKVSVSDYEDVLHPLGFKAVLIKD 243

Query: 250 NELAIPPRK 258
           NELAIPPRK
Sbjct: 255 NELAIPPRK 243

[Close]

BLAST of Lsi01G020070.1 vs. NCBI nr
Match: gi|659069392|ref|XP_008449585.1| (PREDICTED: probable thiol methyltransferase 2 isoform X5 [Cucumis melo])

HSP 1 Score: 356.3 bits (913), Expect = 4.8e-95
Identity = 180/225 (80.00%), Postives = 189/225 (84.00%), Query Frame = 1


Query: 10  AAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEICWEQGLTPW 69
           AAPLQTHR LT+KLRMNQ  TEH ASK NHQSRV EFQKV+  DH+GGW+ICWE GLTPW
Sbjct: 15  AAPLQTHRALTLKLRMNQNSTEHKASKFNHQSRVHEFQKVMGTDHSGGWDICWEHGLTPW 74

Query: 70  DLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKES 129
           DLGQPTP+ISHLCATGALPNGRVLVPGCGSGYDVVQMAC+ERYVVGLDISDNAIQKA   
Sbjct: 75  DLGQPTPLISHLCATGALPNGRVLVPGCGSGYDVVQMACAERYVVGLDISDNAIQKA--- 134

Query: 130 QNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEP 189
                  ELSSSSPNMR+ TFL+EDFFSWRPKELFDLIFDYT           FF AIEP
Sbjct: 135 ------IELSSSSPNMRHCTFLREDFFSWRPKELFDLIFDYT-----------FFCAIEP 194

Query: 190 VMRLAWGQQIQRLLKPNGELITLMFPI-DDHSGGPPYKVSVSDYE 234
           VMR  WGQQIQRLLKPNGELITLMFPI DDHSGGPPYKVSVS+ E
Sbjct: 195 VMRQVWGQQIQRLLKPNGELITLMFPIDDDHSGGPPYKVSVSEDE 219

[Close]

BLAST of Lsi01G020070.1 vs. NCBI nr
Match: gi|659069388|ref|XP_008449569.1| (PREDICTED: probable thiol methyltransferase 2 isoform X4 [Cucumis melo])

HSP 1 Score: 327.0 bits (837), Expect = 3.1e-86
Identity = 164/206 (79.61%), Postives = 172/206 (83.50%), Query Frame = 1


Query: 10  AAPLQTHRNLTMKLRMNQQYTEHNASKSNHQSRVDEFQKVVRKDHTGGWEICWEQGLTPW 69
           AAPLQTHR LT+KLRMNQ  TEH ASK NHQSRV EFQKV+  DH+GGW+ICWE GLTPW
Sbjct: 15  AAPLQTHRALTLKLRMNQNSTEHKASKFNHQSRVHEFQKVMGTDHSGGWDICWEHGLTPW 74

Query: 70  DLGQPTPVISHLCATGALPNGRVLVPGCGSGYDVVQMACSERYVVGLDISDNAIQKAKES 129
           DLGQPTP+ISHLCATGALPNGRVLVPGCGSGYDVVQMAC+ERYVVGLDISDNAIQKA   
Sbjct: 75  DLGQPTPLISHLCATGALPNGRVLVPGCGSGYDVVQMACAERYVVGLDISDNAIQKA--- 134

Query: 130 QNIGRTKELSSSSPNMRYFTFLKEDFFSWRPKELFDLIFDYTFQHDRYWKPCRFFSAIEP 189
                  ELSSSSPNMR+ TFL+EDFFSWRPKELFDLIFDYT           FF AIEP
Sbjct: 135 ------IELSSSSPNMRHCTFLREDFFSWRPKELFDLIFDYT-----------FFCAIEP 194

Query: 190 VMRLAWGQQIQRLLKPNGELITLMFP 216
           VMR  WGQQIQRLLKPNGELITLMFP
Sbjct: 195 VMRQVWGQQIQRLLKPNGELITLMFP 200

The following BLAST results are available for this feature:

BLAST of Lsi01G020070.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HOL3_ARATH	6.1e-74	58.23	Probable thiol methyltransferase 2 OS=Arabidopsis thaliana GN=HOL3 PE=1 SV=1	[more]
TMT1_BRAOL	5.9e-69	58.96	Thiocyanate methyltransferase 1 OS=Brassica oleracea GN=TMT1 PE=1 SV=1	[more]
HOL2_ARATH	1.5e-67	58.02	Probable thiol methyltransferase 1 OS=Arabidopsis thaliana GN=HOL2 PE=1 SV=1	[more]
TMT2_BRAOL	2.5e-67	53.75	Probable thiocyanate methyltransferase 2 OS=Brassica oleracea GN=TMT2 PE=2 SV=1	[more]
HOL1_ARATH	7.0e-62	52.81	Thiocyanate methyltransferase 1 OS=Arabidopsis thaliana GN=HOL1 PE=1 SV=1	[more]

BLAST of Lsi01G020070.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KYY5_CUCSA	1.0e-120	81.32	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G439050 PE=4 SV=1	[more]
B9IJ56_POPTR	1.9e-82	66.38	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0017s03980g PE=4 SV=2	[more]
A0A061EXJ7_THECC	8.0e-81	57.30	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein isoform...	[more]
A0A067K092_JATCU	2.3e-80	61.92	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19270 PE=4 SV=1	[more]
A0A0B0MY55_GOSAR	2.0e-79	59.84	Putative thiol methyltransferase 2-like protein OS=Gossypium arboreum GN=F383_05...	[more]

BLAST of Lsi01G020070.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G43940.1	3.4e-75	58.23	S-adenosyl-L-methionine-dependent methyltransferases superfamily pro...	[more]
AT2G43920.1	8.2e-69	58.02	S-adenosyl-L-methionine-dependent methyltransferases superfamily pro...	[more]
AT2G43910.2	3.9e-63	52.81	HARMLESS TO OZONE LAYER 1	[more]

BLAST of Lsi01G020070.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449453195\|ref\|XP_004144344.1\|	1.5e-120	81.32	PREDICTED: probable thiol methyltransferase 2 [Cucumis sativus]	[more]
gi\|659069382\|ref\|XP_008449545.1\|	1.9e-120	83.08	PREDICTED: probable thiol methyltransferase 2 isoform X1 [Cucumis melo]	[more]
gi\|659069384\|ref\|XP_008449554.1\|	2.8e-111	82.73	PREDICTED: probable thiol methyltransferase 2 isoform X2 [Cucumis melo]	[more]
gi\|659069392\|ref\|XP_008449585.1\|	4.8e-95	80.00	PREDICTED: probable thiol methyltransferase 2 isoform X5 [Cucumis melo]	[more]
gi\|659069388\|ref\|XP_008449569.1\|	3.1e-86	79.61	PREDICTED: probable thiol methyltransferase 2 isoform X4 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **Molecular Function**
Term	Definition
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR008854	TPMT

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Lsi01G020070	Lsi01G020070	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Lsi01G020070.1	Lsi01G020070.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi01G020070.1.CDS.1	Lsi01G020070.1.CDS.1	CDS
Lsi01G020070.1.CDS.2	Lsi01G020070.1.CDS.2	CDS
Lsi01G020070.1.CDS.3	Lsi01G020070.1.CDS.3	CDS
Lsi01G020070.1.CDS.4	Lsi01G020070.1.CDS.4	CDS
Lsi01G020070.1.CDS.5	Lsi01G020070.1.CDS.5	CDS
Lsi01G020070.1.CDS.6	Lsi01G020070.1.CDS.6	CDS
Lsi01G020070.1.CDS.7	Lsi01G020070.1.CDS.7	CDS
Lsi01G020070.1.CDS.8	Lsi01G020070.1.CDS.8	CDS
Lsi01G020070.1.CDS.9	Lsi01G020070.1.CDS.9	CDS
Lsi01G020070.1.CDS.10	Lsi01G020070.1.CDS.10	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Lsi01G020070.1.three_prime_UTR.1	Lsi01G020070.1.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008854	TPMT family	PFAM	PF05724	TPMT	coord: 55..259 score: 5.0
IPR008854	TPMT family	PROFILE	PS51585	SAM_MT_TPMT	coord: 55..267 score: 44
None	No IPR available	PANTHER	PTHR32183	FAMILY NOT NAMED	coord: 12..273 score: 2.4E
None	No IPR available	PANTHER	PTHR32183:SF6	THIOCYANATE METHYLTRANSFERASE 1-RELATED	coord: 12..273 score: 2.4E