CmoCh20G001800 (gene) Cucurbita moschata (Rifu)

NameCmoCh20G001800
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu))
Descriptionpeptidyl-prolyl cis-trans isomerases;hydrolases;nucleoside-triphosphatases;ATP binding;nucleotide binding;ATPases
LocationCmo_Chr20 : 897254 .. 901350 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCTGAACTGAGCCTCCCGGAGGCATAAGATTTTCCCTTGTTTGTGAGGCTTTGCTTAACGTTATTTTTCAACCGATCTATTCCATTCAAGCTTCAGCCTCCGATCTCGAGGTAAAGGATTTTCCCGGTAAATTATATGCTTCCTTGGAAATCACCACTTGAGATTTAGGATTTCAATCGTTTGTTTGCTTTGAGATTCCAAGAGTCAAAGATCGATGTTCTAGGGTTTTTGTTTTCCTCGGAATACAATTTGCACGCACTGTTTTAGCCTATCTATGAAACTTGGTTGAAGTTATTCCAGAGATGTATAGCAATTTCAAGGAGCAAGCTATCGAGTACGTGAAGCAGGCGGTACATGAAGATAATGCTGGCAATTACGCGAAAGCCTTCCCCCTGTATATGAACGCCTTGGAGTACTTCAAAACTCATTTGAAGTATGAGAAGAATCCTAAGATCAAGGAAGCCATCACCCAGAAATTCACCGAGTATTTGCGCCGTGCCGAGGAGATACGTGCGGTTCTCGATGATGGCGGTCCTGGTCCGGCTTCCAATGGGGATGCAGCGGTTGCGACTAAGCCCAAAACGAAGCCAAAGGATGGAGAAGGAGGAGATGGAGAGGATCCAGAACAGACGAAGCTGCGGGCCGGTCTTAATTCCGCAATCATTAGGGAGAAGCCGGATGTCAAGTGGAACGATGTCGCTGGTTTAGAGAGCGCCAAGCAGGCATTGCAAGAGGCGGTAATATTACCCGTTAAGTTCCCGCAGTTCTTTACTGGTTAGTTCCATTTGACTTCTTTCGATATACTTGCATATTGATCTACGTTCTTCGTTTGCAATGGTCTTTATCACGTCGGTAAAGTTGCGTTGTCTCCTTGAGATTGGGATTTTATTCCTTTCGCATTTGGGTCTGATTTGGTGCGTAATGATTACGATTATGCTGGATTGAATGCTTGGTTGTCCTTTTTGTGCTCCTCTTTCTTGGAGTTGGAAGGAGATCCTGAGCACCATCTTCTTGTTTAATTCTAATGGTGAATCTTACCCTAATCAATTGCTTGATTCTACCCCTTCAATTACTGAATTGGACATGGAATAAACATGTGTAGAAGACTTGTTCTCATTTTCAATTATGAAGGTTTCTGCTTAGTTTTTAAATAAAAACATTTTCAAGATAATTATCTGGCTTTGTTTAGGCAAGAGGCGACCATGGCGAGCTTTCTTGTTATATGGGCCTCCTGGAACTGGAAAATCATACTTGGCAAAGGCTGTTGCGACGGAGGCCGACTCAACATTTTTCAGGTAATGGTCCTTCAAGCATATGAACTTGTCTTCTGTTATTTTTGTTTAGTCGTGACGCAATATATTTCTTCACTTACCTTGCTGTTTTCTTATAAGGTGACCCTGTGAGAAACTTACAACCCTCAAATACTCATGCAGCTCTTTTTTTTTTTTTTTTTAATTTATGGCATCAACAAAATTTATGACATGTGAGCAGCTAGTATGAAAGAGTGCAGCGAATATGAACAATATCATTTTGAAGCGATGTCTCTTATTTGCATTAGAGTTGAGGATCCAAGAGGTTAATAACAAATTTAGCTGTGTGGAATATCATTTTATAAATGTTCTATTCAACATGTCTAATAACAGATTTTGCTTTCATTCCCATTATATTCAATTGCAGTGCATACACACTCCACTTTCTATCTGGATATCTTTGGCGGTTATTTGAATGTGTTTTTAGTTATCACTAATCTCAATCATTTGCTGGTTCTATACCTTTTGTTTTAAATTGAGTATTTTTTCATTTTCTGCTGATAGCATTTCTTCTTCGGACCTTGTCTCGAAATGGATGGGTGAAAGTGAAAAGTTAGTTTCAAATCTTTTCGAAATGGCTCGTGATAGCGCTCCTTCTATCATCTTTATTGATGAAATAGATTCTCTGTGTGGTCAACGAGGTGAAGGTAATGAAAGTGAAGCTTCAAGACGCATTAAGACAGAACTTCTTGTGCAAATGCAGGTATTTTTTGTAGAGTAACAGTGTTAGTATTTTGTGTATTGAAGGAGGGGGTTGATTATTATTATTATCTTATGCACCCTAAGCCCATTCCATTGTCTTGAGAATTCTCATTTTCTGAAAATGTGTCCTTACTTGCTGCTGACCAACATTCAAGTGTTTTCTTGCTAGTTGAAAGTTGACTTCAGTTAGATCTGAAGACTTTTGTAATAATTAACAGTCCAATTACTTGTTTATGTGATTCTGCCCAACGACCTGGGTTTTCACTGCTTACACCTAAGACTTTAGAATATCTGGTTTGTTTCATATCTTGCAAAAAAATCCAAAAATTTTCAAACTTCTCCATTTGTATGGGTTTGCTACTGTGTTCACATTTTAAAATTTGGTAGTTGGGCAATGGACAAAGATGGGGACCTGGATGAGCAAGCCTGAGGCCGAGGGGTTTGAACTCATTATAACTTTCTCTTATTGTTTTCTTAACGTGCCCACCCTTTAATGTGTGTTTCACATGTTTCATGCATGCTGTTTGTTTTTTATTTTTCTGTTTGTTTGTTGCGAATGATCTCTGTATTAAATAATTTGTCAGATGAATTTTGATGCTTAGTGAAATTTGCACAATGAAAATGTAGTTTATTTTCTAATTACAGTTAAATAATCTACTCATGTATCACTAATTAAGCAATAGCTTATTGTGTCTGTGTTATTTTATTTTGGAGAAGATTACTTTGTGATTCACTTCAGGAATAAATTTAGGGAAAGGGGATGGAAATTATTTGGACATTTGGTGGGTTATAAATTTTCGGTTATGTTAATGTTTTTGGAAGCCTGTTTTTAAGAACTTGAAATCACTATTCACCAGTGTGTGATTGCATAAGAAGAACAACTTTACTTCTTTTAAAAAAAGTTCGTCCATGAGAGCAAATTGGACAAGAAAAAAAAAACATCTAAAAAATAGTTATCCAAATCCAAATATGCTATCATCGATTTTTTTTCTTCCAAAAAATAGAAAATGTATTTAGAAATGGTTTCGAAACAAACTCCATGGGTATTATCTCACTTTACCTTGTTTGTTACCAATGTTATTTGAAAATTGAAGCATATATTTCTTGCATTAATAGGGTGTAGGACACAATGATCAAAAGGTTCTTGTCCTTGCTGCAACAAATACTCCCTATGCTTTGGATCAGGTGAAGATGAAAATTGTCTATTTCCATATCATGTAAAGGATTACGGAAGTGTAATGCATATTTGCTGATAAGTAGTGTTTACAGGCCATTCGTCGACGATTCGACAAGCGGATATACATCCCTCTTCCCGACGCAAAAGCCAGACAGCACATGTTCAAAGTTCTGGGAATTATATTTTATTTCGTCCTGTAAAAAGCTAGATTTTTCTTTTTCGCCTGGAATGAACTCTGCTAAATTCCTAGATGTATTTAAATAGGTGCATCTGGGGGATACACCACATAATTTAACGGAAGCAGATTTTGAAAGCTTAGCCCGCAGGACAGATGGTTTCTCTGGTTCAGATGTTTCTGTTTGTGTAAGTGTATAATAGTAGTTGGAGGAACTGAATTATTCATTGAATTGTAATCAGAGATTAGTCAGAACATAGTTCATGGACCCCTTCTTAAATTTGCAGGTGAAGGATGTGCTCTTTGAGCCTGTTCGTAAAACACAAGATGCTATGTTCTTCATCAAGACTCCTGATGGTATGTGGGTACCTTGTGGACCAAAGCAACAAGGCGCTGTCCAAATTACCATGCAAGAGTTGGCAGCCAAAGGACTCGCCTCAAAGGTACATCGCATATTACCATCCTAAATGAGCGTTTGGATTTAAAATTTGATATTTGACGTGCAAGTACAGGTGCTCCTTGTGGCTGCTTGTTGCTAGGTACTCGCAATTTTCATTTAGTGAGAAATATATATATTATTAACATCTGTTACTTGTTTGGGTGCAGATTCTTCCTCCTCCAATTACAAGAACAGACTTCGACAAGGTTCTAGCTAGACAAAGGCCTACGGTGAGTAAATCTGATCTGGAGATTCACGAAAAGTTTACGAAGGAGTTTGGGGAGGAAGGTTGA

mRNA sequence

TGCTGAACTGAGCCTCCCGGAGGCATAAGATTTTCCCTTGTTTGTGAGGCTTTGCTTAACGTTATTTTTCAACCGATCTATTCCATTCAAGCTTCAGCCTCCGATCTCGAGGTAAAGGATTTTCCCGGTAAATTATATGCTTCCTTGGAAATCACCACTTGAGATTTAGGATTTCAATCGTTTGTTTGCTTTGAGATTCCAAGAGTCAAAGATCGATGTTCTAGGGTTTTTGTTTTCCTCGGAATACAATTTGCACGCACTGTTTTAGCCTATCTATGAAACTTGGTTGAAGTTATTCCAGAGATGTATAGCAATTTCAAGGAGCAAGCTATCGAGTACGTGAAGCAGGCGGTACATGAAGATAATGCTGGCAATTACGCGAAAGCCTTCCCCCTGTATATGAACGCCTTGGAGTACTTCAAAACTCATTTGAAGTATGAGAAGAATCCTAAGATCAAGGAAGCCATCACCCAGAAATTCACCGAGTATTTGCGCCGTGCCGAGGAGATACGTGCGGTTCTCGATGATGGCGGTCCTGGTCCGGCTTCCAATGGGGATGCAGCGGTTGCGACTAAGCCCAAAACGAAGCCAAAGGATGGAGAAGGAGGAGATGGAGAGGATCCAGAACAGACGAAGCTGCGGGCCGGTCTTAATTCCGCAATCATTAGGGAGAAGCCGGATGTCAAGTGGAACGATGTCGCTGGTTTAGAGAGCGCCAAGCAGGCATTGCAAGAGGCGGTAATATTACCCGTTAAGTTCCCGCAGTTCTTTACTGGCAAGAGGCGACCATGGCGAGCTTTCTTGTTATATGGGCCTCCTGGAACTGGAAAATCATACTTGGCAAAGGCTGTTGCGACGGAGGCCGACTCAACATTTTTCAGCATTTCTTCTTCGGACCTTGTCTCGAAATGGATGGGTGAAAGTGAAAAGTTAGTTTCAAATCTTTTCGAAATGGCTCGTGATAGCGCTCCTTCTATCATCTTTATTGATGAAATAGATTCTCTGTGTGGTCAACGAGGTGAAGGTAATGAAAGTGAAGCTTCAAGACGCATTAAGACAGAACTTCTTGTGCAAATGCAGGGTGTAGGACACAATGATCAAAAGGTTCTTGTCCTTGCTGCAACAAATACTCCCTATGCTTTGGATCAGGCCATTCGTCGACGATTCGACAAGCGGATATACATCCCTCTTCCCGACGCAAAAGCCAGACAGCACATGTTCAAAGTGCATCTGGGGGATACACCACATAATTTAACGGAAGCAGATTTTGAAAGCTTAGCCCGCAGGACAGATGGTTTCTCTGGTTCAGATGTTTCTGTTTGTGTGAAGGATGTGCTCTTTGAGCCTGTTCGTAAAACACAAGATGCTATGTTCTTCATCAAGACTCCTGATGGTATGTGGGTACCTTGTGGACCAAAGCAACAAGGCGCTGTCCAAATTACCATGCAAGAGTTGGCAGCCAAAGGACTCGCCTCAAAGATTCTTCCTCCTCCAATTACAAGAACAGACTTCGACAAGGTTCTAGCTAGACAAAGGCCTACGGTGAGTAAATCTGATCTGGAGATTCACGAAAAGTTTACGAAGGAGTTTGGGGAGGAAGGTTGA

Coding sequence (CDS)

ATGTATAGCAATTTCAAGGAGCAAGCTATCGAGTACGTGAAGCAGGCGGTACATGAAGATAATGCTGGCAATTACGCGAAAGCCTTCCCCCTGTATATGAACGCCTTGGAGTACTTCAAAACTCATTTGAAGTATGAGAAGAATCCTAAGATCAAGGAAGCCATCACCCAGAAATTCACCGAGTATTTGCGCCGTGCCGAGGAGATACGTGCGGTTCTCGATGATGGCGGTCCTGGTCCGGCTTCCAATGGGGATGCAGCGGTTGCGACTAAGCCCAAAACGAAGCCAAAGGATGGAGAAGGAGGAGATGGAGAGGATCCAGAACAGACGAAGCTGCGGGCCGGTCTTAATTCCGCAATCATTAGGGAGAAGCCGGATGTCAAGTGGAACGATGTCGCTGGTTTAGAGAGCGCCAAGCAGGCATTGCAAGAGGCGGTAATATTACCCGTTAAGTTCCCGCAGTTCTTTACTGGCAAGAGGCGACCATGGCGAGCTTTCTTGTTATATGGGCCTCCTGGAACTGGAAAATCATACTTGGCAAAGGCTGTTGCGACGGAGGCCGACTCAACATTTTTCAGCATTTCTTCTTCGGACCTTGTCTCGAAATGGATGGGTGAAAGTGAAAAGTTAGTTTCAAATCTTTTCGAAATGGCTCGTGATAGCGCTCCTTCTATCATCTTTATTGATGAAATAGATTCTCTGTGTGGTCAACGAGGTGAAGGTAATGAAAGTGAAGCTTCAAGACGCATTAAGACAGAACTTCTTGTGCAAATGCAGGGTGTAGGACACAATGATCAAAAGGTTCTTGTCCTTGCTGCAACAAATACTCCCTATGCTTTGGATCAGGCCATTCGTCGACGATTCGACAAGCGGATATACATCCCTCTTCCCGACGCAAAAGCCAGACAGCACATGTTCAAAGTGCATCTGGGGGATACACCACATAATTTAACGGAAGCAGATTTTGAAAGCTTAGCCCGCAGGACAGATGGTTTCTCTGGTTCAGATGTTTCTGTTTGTGTGAAGGATGTGCTCTTTGAGCCTGTTCGTAAAACACAAGATGCTATGTTCTTCATCAAGACTCCTGATGGTATGTGGGTACCTTGTGGACCAAAGCAACAAGGCGCTGTCCAAATTACCATGCAAGAGTTGGCAGCCAAAGGACTCGCCTCAAAGATTCTTCCTCCTCCAATTACAAGAACAGACTTCGACAAGGTTCTAGCTAGACAAAGGCCTACGGTGAGTAAATCTGATCTGGAGATTCACGAAAAGTTTACGAAGGAGTTTGGGGAGGAAGGTTGA
BLAST of CmoCh20G001800 vs. Swiss-Prot
Match: VPS4_ARATH (Protein SUPPRESSOR OF K(+) TRANSPORT GROWTH DEFECT 1 OS=Arabidopsis thaliana GN=SKD1 PE=1 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 9.5e-231
Identity = 392/435 (90.11%), Postives = 419/435 (96.32%), Query Frame = 1

Query: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60
           MYSNFKEQAIEYVKQAVHEDNAGNY KAFPLYMNALEYFKTHLKYEKNPKI+EAITQKFT
Sbjct: 1   MYSNFKEQAIEYVKQAVHEDNAGNYNKAFPLYMNALEYFKTHLKYEKNPKIREAITQKFT 60

Query: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGG--DGEDPEQTKLRAGLNS 120
           EYLRRAEEIRAVLD+GG GP SNGDAAVAT+PKTKPKDGEGG  DGEDPEQ+KLRAGLNS
Sbjct: 61  EYLRRAEEIRAVLDEGGSGPGSNGDAAVATRPKTKPKDGEGGGKDGEDPEQSKLRAGLNS 120

Query: 121 AIIREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSY 180
           AI+REKP++KW+DVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSY
Sbjct: 121 AIVREKPNIKWSDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSY 180

Query: 181 LAKAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQR 240
           LAKAVATEADSTFFS+SSSDLVSKWMGESEKLVSNLFEMAR+SAPSIIF+DEIDSLCG R
Sbjct: 181 LAKAVATEADSTFFSVSSSDLVSKWMGESEKLVSNLFEMARESAPSIIFVDEIDSLCGTR 240

Query: 241 GEGNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPD 300
           GEGNESEASRRIKTELLVQMQGVGHND+KVLVLAATNTPYALDQAIRRRFDKRIYIPLP+
Sbjct: 241 GEGNESEASRRIKTELLVQMQGVGHNDEKVLVLAATNTPYALDQAIRRRFDKRIYIPLPE 300

Query: 301 AKARQHMFKVHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMFF 360
           AKARQHMFKVHLGDTPHNLTE DFE L ++T+GFSGSDVSVCVKDVLFEPVRKTQDAMFF
Sbjct: 301 AKARQHMFKVHLGDTPHNLTEPDFEYLGQKTEGFSGSDVSVCVKDVLFEPVRKTQDAMFF 360

Query: 361 IKTPDGMWVPCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSD 420
            K+PDG W+PCGP+  GA+Q TMQ+LA KGLA KI+PPPITRTDF+KVLARQRPTVSKSD
Sbjct: 361 FKSPDGTWMPCGPRHPGAIQTTMQDLATKGLAEKIIPPPITRTDFEKVLARQRPTVSKSD 420

Query: 421 LEIHEKFTKEFGEEG 434
           L++HE+FT+EFGEEG
Sbjct: 421 LDVHERFTQEFGEEG 435

BLAST of CmoCh20G001800 vs. Swiss-Prot
Match: VPS4_CANAL (Vacuolar protein sorting-associated protein 4 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=VPS4 PE=2 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 2.2e-126
Identity = 238/440 (54.09%), Postives = 309/440 (70.23%), Query Frame = 1

Query: 3   SNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFTEY 62
           S+F  + I+ V++A+  D A  Y +A+ LY N L+Y    +KYEKNPK KE +  KFTEY
Sbjct: 5   SDFLSKGIDLVQKAIDADTATRYEEAYKLYYNGLDYLMLAIKYEKNPKSKELVKSKFTEY 64

Query: 63  LRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGG----DGEDPEQTKLRAGLNS 122
           L RAE+++  L+       S  +++V    K K  +G+G     D +D +  KLR  L  
Sbjct: 65  LTRAEQLKDHLEKQAQNK-STAESSVNGSTKAKKSNGDGNGSGDDNDDADTKKLRGALAG 124

Query: 123 AIIREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSY 182
           AI+ EKP+VKW+D+AGL++AK+AL+EAVILPVKFPQ F G R+P    LLYGPPGTGKSY
Sbjct: 125 AILSEKPNVKWSDIAGLDAAKEALKEAVILPVKFPQLFVGNRKPTSGILLYGPPGTGKSY 184

Query: 183 LAKAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQR 242
           LAKAVATEA+STFFS+SSSDLVSKWMGESE+LV  LF MAR++ PSIIFIDE+D+LCG R
Sbjct: 185 LAKAVATEANSTFFSVSSSDLVSKWMGESERLVKQLFTMARENKPSIIFIDEVDALCGPR 244

Query: 243 GEGNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPD 302
           GEG ESEASRRIKTELLVQM GVG++ Q VLVL ATN P+ LD A+RRRF++RIYI LPD
Sbjct: 245 GEG-ESEASRRIKTELLVQMNGVGNDSQGVLVLGATNIPWQLDAAVRRRFERRIYIALPD 304

Query: 303 AKARQHMFKVHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMFF 362
            +AR  MF++++GD P   T  D+ +LA  TDG+SG DV+V V+D L +P+RK Q A  F
Sbjct: 305 VEARTRMFEINIGDVPCECTPHDYRTLAEMTDGYSGHDVAVVVRDALMQPIRKIQQATHF 364

Query: 363 ---IKTPDG--MWVPCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQRPT 422
              I   DG     PC P  +GA ++   +LA      ++  PP+T  DF K +   RPT
Sbjct: 365 KPVIDETDGKEKLTPCSPGDEGAREMNWMDLA----TDELKEPPLTIKDFIKAIKNNRPT 424

Query: 423 VSKSDLEIHEKFTKEFGEEG 434
           V+++D+  H KFT++FG+EG
Sbjct: 425 VNEADIAQHVKFTEDFGQEG 438

BLAST of CmoCh20G001800 vs. Swiss-Prot
Match: VPS4B_HUMAN (Vacuolar protein sorting-associated protein 4B OS=Homo sapiens GN=VPS4B PE=1 SV=2)

HSP 1 Score: 448.7 bits (1153), Expect = 7.0e-125
Identity = 242/443 (54.63%), Postives = 314/443 (70.88%), Query Frame = 1

Query: 7   EQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYE-KNPKIKEAITQKFTEYLRR 66
           ++AI+   +A  ED AGNY +A  LY +A++YF   +KYE +  K K++I  K TEYL R
Sbjct: 9   QKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDR 68

Query: 67  AEEIRAVL---DDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQTKLRAGLNSAIIR 126
           AE+++  L   +     P   G  + A + K    DGEG + +DPE+ KL+  L  AI+ 
Sbjct: 69  AEKLKEYLKNKEKKAQKPVKEGQPSPADE-KGNDSDGEG-ESDDPEKKKLQNQLQGAIVI 128

Query: 127 EKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLAKA 186
           E+P+VKW+DVAGLE AK+AL+EAVILP+KFP  FTGKR PWR  LL+GPPGTGKSYLAKA
Sbjct: 129 ERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKA 188

Query: 187 VATEAD-STFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQRGEG 246
           VATEA+ STFFSISSSDLVSKW+GESEKLV NLF++AR++ PSIIFIDEIDSLCG R E 
Sbjct: 189 VATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSE- 248

Query: 247 NESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDAKA 306
           NESEA+RRIKTE LVQMQGVG ++  +LVL ATN P+ LD AIRRRF+KRIYIPLP+  A
Sbjct: 249 NESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHA 308

Query: 307 RQHMFKVHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMFFIKT 366
           R  MFK+HLG T ++LTEADF  L R+TDG+SG+D+S+ V+D L +PVRK Q A  F K 
Sbjct: 309 RAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKV 368

Query: 367 -----------PDGMWVPCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQ 426
                       D +  PC P   GA+++T  ++       K+L P ++ +D  + L+  
Sbjct: 369 RGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVP----GDKLLEPVVSMSDMLRSLSNT 428

Query: 427 RPTVSKSDLEIHEKFTKEFGEEG 434
           +PTV++ DL   +KFT++FG+EG
Sbjct: 429 KPTVNEHDLLKLKKFTEDFGQEG 444

BLAST of CmoCh20G001800 vs. Swiss-Prot
Match: VPS4B_BOVIN (Vacuolar protein sorting-associated protein 4B OS=Bos taurus GN=VPS4B PE=2 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 2.0e-124
Identity = 242/443 (54.63%), Postives = 314/443 (70.88%), Query Frame = 1

Query: 7   EQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYE-KNPKIKEAITQKFTEYLRR 66
           ++AI+   +A  ED AGNY +A  LY +A++YF   +KYE +  K K++I  K TEYL R
Sbjct: 9   QKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDR 68

Query: 67  AEEIRAVL---DDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQTKLRAGLNSAIIR 126
           AE+++  L   +     P   G  A A + K    DGEG + +DPE+ KL+  L  AI+ 
Sbjct: 69  AEKLKEYLKKREKKPQKPVKEGQPAPADE-KGNDSDGEG-ESDDPEKKKLQNQLQGAIVI 128

Query: 127 EKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLAKA 186
           E+P+VKW+DVAGLE AK+AL+EAVILP+KFP  FTGKR PWR  LL+GPPGTGKSYLAKA
Sbjct: 129 ERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKA 188

Query: 187 VATEAD-STFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQRGEG 246
           VATEA+ STFFSISSSDLVSKW+GESEKLV NLF++AR++ PSIIFIDEIDSLCG R E 
Sbjct: 189 VATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSE- 248

Query: 247 NESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDAKA 306
           NESEA+RRIKTE LVQMQGVG ++  +LVL ATN P+ LD AIRRRF+KRIYIPLP+A A
Sbjct: 249 NESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEAHA 308

Query: 307 RQHMFKVHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMFFIKT 366
           R  MFK+HLG T ++LTEADF  L ++T+G+SG+D+S+ V+D L +PVRK Q A  F K 
Sbjct: 309 RAAMFKLHLGTTQNSLTEADFRDLGKKTEGYSGADISIIVRDALMQPVRKVQSATHFKKV 368

Query: 367 -----------PDGMWVPCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQ 426
                       D +  PC P   GA+++T  ++       K+L P +  +D  + L+  
Sbjct: 369 RGPSRADPNNIVDDLLTPCSPGDPGAIEMTWMDVP----GDKLLEPVVCMSDMLRSLSST 428

Query: 427 RPTVSKSDLEIHEKFTKEFGEEG 434
           +PTV++ DL   +KFT++FG+EG
Sbjct: 429 KPTVNEHDLLKLKKFTEDFGQEG 444

BLAST of CmoCh20G001800 vs. Swiss-Prot
Match: VPS4B_PONAB (Vacuolar protein sorting-associated protein 4B OS=Pongo abelii GN=VPS4B PE=2 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 5.9e-124
Identity = 240/443 (54.18%), Postives = 314/443 (70.88%), Query Frame = 1

Query: 7   EQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYE-KNPKIKEAITQKFTEYLRR 66
           ++AI+   +A  ED AGNY +A  LY +A++YF   +KYE +  K K++I  + TEYL R
Sbjct: 9   QKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAQCTEYLDR 68

Query: 67  AEEIRAVL---DDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQTKLRAGLNSAIIR 126
           AE+++  L   +     P   G  + A + K    DGEG + +DPE+ KL+  L  AI+ 
Sbjct: 69  AEKLKEYLKNKEKKAQKPVKEGQPSPADE-KGNDSDGEG-ESDDPEKKKLQNQLQGAIVI 128

Query: 127 EKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLAKA 186
           E+P+VKW+DVAGLE AK+AL+EAVILP+KFP  FTGKR PWR  LL+GPPGTGKSYLAKA
Sbjct: 129 ERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKA 188

Query: 187 VATEAD-STFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQRGEG 246
           VATEA+ STFFSISSSDLVSKW+GESEKLV NLF++AR++ PSIIFIDEIDSLCG R E 
Sbjct: 189 VATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSE- 248

Query: 247 NESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDAKA 306
           NESEA+RRIKTE LVQM+GVG ++  +LVL ATN P+ LD AIRRRF+KRIYIPLP+  A
Sbjct: 249 NESEAARRIKTEFLVQMRGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHA 308

Query: 307 RQHMFKVHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMFFIKT 366
           R  MFK+HLG T ++LTEADF  L R+TDG+SG+D+S+ V+D L +PVRK Q A  F K 
Sbjct: 309 RAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKV 368

Query: 367 -----------PDGMWVPCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQ 426
                       D +  PC P   GA+++T  ++       K+L P ++ +D  + L+  
Sbjct: 369 RGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVP----GDKLLEPVVSMSDMLRSLSNT 428

Query: 427 RPTVSKSDLEIHEKFTKEFGEEG 434
           +PTV++ DL   +KFT++FG+EG
Sbjct: 429 KPTVNEHDLLKLKKFTEDFGQEG 444

BLAST of CmoCh20G001800 vs. TrEMBL
Match: A0A0A0KGS6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G152960 PE=3 SV=1)

HSP 1 Score: 852.0 bits (2200), Expect = 3.1e-244
Identity = 424/433 (97.92%), Postives = 430/433 (99.31%), Query Frame = 1

Query: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60
           MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT
Sbjct: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60

Query: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQTKLRAGLNSAI 120
           EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQ KLRAGLNSAI
Sbjct: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQAKLRAGLNSAI 120

Query: 121 IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA 180
           IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA
Sbjct: 121 IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA 180

Query: 181 KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQRGE 240
           KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLF+MARDSAPSIIFIDEIDSLCGQRGE
Sbjct: 181 KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFQMARDSAPSIIFIDEIDSLCGQRGE 240

Query: 241 GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDAK 300
           GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPD K
Sbjct: 241 GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDLK 300

Query: 301 ARQHMFKVHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMFFIK 360
           ARQHMFKVHLGDTPHNLTEADFE+LAR+TDGFSGSD+SVCVKDVLFEPVRKTQDAMFFI 
Sbjct: 301 ARQHMFKVHLGDTPHNLTEADFENLARKTDGFSGSDISVCVKDVLFEPVRKTQDAMFFIM 360

Query: 361 TPDGMWVPCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE 420
           TPDGMWVPCGPKQQGAVQI+MQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE
Sbjct: 361 TPDGMWVPCGPKQQGAVQISMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE 420

Query: 421 IHEKFTKEFGEEG 434
           IHE+FTKEFGEEG
Sbjct: 421 IHERFTKEFGEEG 433

BLAST of CmoCh20G001800 vs. TrEMBL
Match: T2HVY3_CUCSA (Vacuolar protein sorting-associated protein 4-like OS=Cucumis sativus var. sativus GN=VPS4 like PE=3 SV=1)

HSP 1 Score: 852.0 bits (2200), Expect = 3.1e-244
Identity = 424/433 (97.92%), Postives = 430/433 (99.31%), Query Frame = 1

Query: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60
           MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT
Sbjct: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60

Query: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQTKLRAGLNSAI 120
           EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQ KLRAGLNSAI
Sbjct: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQAKLRAGLNSAI 120

Query: 121 IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA 180
           IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA
Sbjct: 121 IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA 180

Query: 181 KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQRGE 240
           KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLF+MARDSAPSIIFIDEIDSLCGQRGE
Sbjct: 181 KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFQMARDSAPSIIFIDEIDSLCGQRGE 240

Query: 241 GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDAK 300
           GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPD K
Sbjct: 241 GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDLK 300

Query: 301 ARQHMFKVHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMFFIK 360
           ARQHMFKVHLGDTPHNLTEADFE+LAR+TDGFSGSD+SVCVKDVLFEPVRKTQDAMFFI 
Sbjct: 301 ARQHMFKVHLGDTPHNLTEADFENLARKTDGFSGSDISVCVKDVLFEPVRKTQDAMFFIM 360

Query: 361 TPDGMWVPCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE 420
           TPDGMWVPCGPKQQGAVQI+MQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE
Sbjct: 361 TPDGMWVPCGPKQQGAVQISMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE 420

Query: 421 IHEKFTKEFGEEG 434
           IHE+FTKEFGEEG
Sbjct: 421 IHERFTKEFGEEG 433

BLAST of CmoCh20G001800 vs. TrEMBL
Match: T2HVB0_CUCSA (Vacuolar protein sorting-associated protein 4-like OS=Cucumis sativus var. sativus GN=VPS4 like PE=3 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 7.5e-243
Identity = 422/433 (97.46%), Postives = 429/433 (99.08%), Query Frame = 1

Query: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60
           MYSNFKEQAIEYVKQAVHEDNAGNYAKA PLY+NALEYFKTHLKYEKNPKIKEAITQKFT
Sbjct: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKASPLYINALEYFKTHLKYEKNPKIKEAITQKFT 60

Query: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQTKLRAGLNSAI 120
           EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQ KLRAGLNSAI
Sbjct: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQAKLRAGLNSAI 120

Query: 121 IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA 180
           IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA
Sbjct: 121 IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA 180

Query: 181 KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQRGE 240
           KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLF+MARDSAPSIIFIDEIDSLCGQRGE
Sbjct: 181 KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFQMARDSAPSIIFIDEIDSLCGQRGE 240

Query: 241 GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDAK 300
           GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPD K
Sbjct: 241 GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDLK 300

Query: 301 ARQHMFKVHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMFFIK 360
           ARQHMFKVHLGDTPHNLTEADFE+LAR+TDGFSGSD+SVCVKDVLFEPVRKTQDAMFFI 
Sbjct: 301 ARQHMFKVHLGDTPHNLTEADFENLARKTDGFSGSDISVCVKDVLFEPVRKTQDAMFFIM 360

Query: 361 TPDGMWVPCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE 420
           TPDGMWVPCGPKQQGAVQI+MQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE
Sbjct: 361 TPDGMWVPCGPKQQGAVQISMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE 420

Query: 421 IHEKFTKEFGEEG 434
           IHE+FTKEFGEEG
Sbjct: 421 IHERFTKEFGEEG 433

BLAST of CmoCh20G001800 vs. TrEMBL
Match: I1LTU5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_12G183700 PE=3 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 2.0e-235
Identity = 408/436 (93.58%), Postives = 425/436 (97.48%), Query Frame = 1

Query: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60
           MYSNFKEQAIEYVKQAV EDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT
Sbjct: 1   MYSNFKEQAIEYVKQAVQEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60

Query: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGE---GGDGEDPEQTKLRAGLN 120
           EYLRRAEEIRAVLDDGGPGPASNGDAAVA +PKTKPKDGE   GGDGEDPEQ KLRAGLN
Sbjct: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVAARPKTKPKDGESGGGGDGEDPEQAKLRAGLN 120

Query: 121 SAIIREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKS 180
           SAIIREKP+VKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKS
Sbjct: 121 SAIIREKPNVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKS 180

Query: 181 YLAKAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQ 240
           YLAKAVATEA+STFFS+SSSDLVSKWMGESEKLVSNLFEMAR+SAPSIIFIDEIDSLCGQ
Sbjct: 181 YLAKAVATEAESTFFSVSSSDLVSKWMGESEKLVSNLFEMARESAPSIIFIDEIDSLCGQ 240

Query: 241 RGEGNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLP 300
           RGEGNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLP
Sbjct: 241 RGEGNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLP 300

Query: 301 DAKARQHMFKVHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMF 360
           D KARQHMFKVHLGDTPHNLTE+DFE LA RT+GFSGSD+SVCVKDVLFEPVRKTQDAMF
Sbjct: 301 DLKARQHMFKVHLGDTPHNLTESDFEYLASRTEGFSGSDISVCVKDVLFEPVRKTQDAMF 360

Query: 361 FIKTPDGMWVPCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKS 420
           F+K P+GMW+PCGPKQQGAVQ +MQELAAKGLASKILPPPITRTDF+KVLARQRPTVSK+
Sbjct: 361 FLKNPEGMWIPCGPKQQGAVQTSMQELAAKGLASKILPPPITRTDFEKVLARQRPTVSKA 420

Query: 421 DLEIHEKFTKEFGEEG 434
           DL++HE+FTKEFGEEG
Sbjct: 421 DLDVHERFTKEFGEEG 436

BLAST of CmoCh20G001800 vs. TrEMBL
Match: M5WHD1_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005989mg PE=3 SV=1)

HSP 1 Score: 821.2 bits (2120), Expect = 5.8e-235
Identity = 407/433 (94.00%), Postives = 424/433 (97.92%), Query Frame = 1

Query: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60
           MYSNFKEQAIEYVKQAV EDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT
Sbjct: 1   MYSNFKEQAIEYVKQAVQEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60

Query: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQTKLRAGLNSAI 120
           EYLRRAEEIRAVLDDGGPGPASNGDAAVAT+PKTKPKDGEGGDGEDPEQ KLRAGLNSAI
Sbjct: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATRPKTKPKDGEGGDGEDPEQAKLRAGLNSAI 120

Query: 121 IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA 180
           IREKP+VKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA
Sbjct: 121 IREKPNVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA 180

Query: 181 KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQRGE 240
           KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLF+MARDSAPSIIFIDEIDSLCG RGE
Sbjct: 181 KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFQMARDSAPSIIFIDEIDSLCGTRGE 240

Query: 241 GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDAK 300
           GNESEASRRIKTELLVQMQGVG NDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPD K
Sbjct: 241 GNESEASRRIKTELLVQMQGVGTNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDLK 300

Query: 301 ARQHMFKVHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMFFIK 360
           ARQHMFKVHLGDTP+NL+E+DFESLAR+T+GFSGSDV+VCVKDVLFEPVRKTQDAMFF K
Sbjct: 301 ARQHMFKVHLGDTPNNLSESDFESLARKTEGFSGSDVAVCVKDVLFEPVRKTQDAMFFFK 360

Query: 361 TPDGMWVPCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE 420
           + D MW+PCGPKQ GA+QITMQELAAKGLAS+ILPPPIT+TDFDKVLARQRPTVSKSDL+
Sbjct: 361 SADNMWIPCGPKQPGAIQITMQELAAKGLASQILPPPITKTDFDKVLARQRPTVSKSDLD 420

Query: 421 IHEKFTKEFGEEG 434
           +HE+FTKEFGEEG
Sbjct: 421 VHERFTKEFGEEG 433

BLAST of CmoCh20G001800 vs. TAIR10
Match: AT2G27600.1 (AT2G27600.1 AAA-type ATPase family protein)

HSP 1 Score: 800.4 bits (2066), Expect = 5.4e-232
Identity = 392/435 (90.11%), Postives = 419/435 (96.32%), Query Frame = 1

Query: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60
           MYSNFKEQAIEYVKQAVHEDNAGNY KAFPLYMNALEYFKTHLKYEKNPKI+EAITQKFT
Sbjct: 1   MYSNFKEQAIEYVKQAVHEDNAGNYNKAFPLYMNALEYFKTHLKYEKNPKIREAITQKFT 60

Query: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGG--DGEDPEQTKLRAGLNS 120
           EYLRRAEEIRAVLD+GG GP SNGDAAVAT+PKTKPKDGEGG  DGEDPEQ+KLRAGLNS
Sbjct: 61  EYLRRAEEIRAVLDEGGSGPGSNGDAAVATRPKTKPKDGEGGGKDGEDPEQSKLRAGLNS 120

Query: 121 AIIREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSY 180
           AI+REKP++KW+DVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSY
Sbjct: 121 AIVREKPNIKWSDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSY 180

Query: 181 LAKAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQR 240
           LAKAVATEADSTFFS+SSSDLVSKWMGESEKLVSNLFEMAR+SAPSIIF+DEIDSLCG R
Sbjct: 181 LAKAVATEADSTFFSVSSSDLVSKWMGESEKLVSNLFEMARESAPSIIFVDEIDSLCGTR 240

Query: 241 GEGNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPD 300
           GEGNESEASRRIKTELLVQMQGVGHND+KVLVLAATNTPYALDQAIRRRFDKRIYIPLP+
Sbjct: 241 GEGNESEASRRIKTELLVQMQGVGHNDEKVLVLAATNTPYALDQAIRRRFDKRIYIPLPE 300

Query: 301 AKARQHMFKVHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMFF 360
           AKARQHMFKVHLGDTPHNLTE DFE L ++T+GFSGSDVSVCVKDVLFEPVRKTQDAMFF
Sbjct: 301 AKARQHMFKVHLGDTPHNLTEPDFEYLGQKTEGFSGSDVSVCVKDVLFEPVRKTQDAMFF 360

Query: 361 IKTPDGMWVPCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSD 420
            K+PDG W+PCGP+  GA+Q TMQ+LA KGLA KI+PPPITRTDF+KVLARQRPTVSKSD
Sbjct: 361 FKSPDGTWMPCGPRHPGAIQTTMQDLATKGLAEKIIPPPITRTDFEKVLARQRPTVSKSD 420

Query: 421 LEIHEKFTKEFGEEG 434
           L++HE+FT+EFGEEG
Sbjct: 421 LDVHERFTQEFGEEG 435

BLAST of CmoCh20G001800 vs. TAIR10
Match: AT1G80350.1 (AT1G80350.1 P-loop containing nucleoside triphosphate hydrolases superfamily protein)

HSP 1 Score: 269.6 bits (688), Expect = 3.3e-72
Identity = 149/362 (41.16%), Postives = 224/362 (61.88%), Query Frame = 1

Query: 76  GGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQTKLRAGLNSAIIREKPDVKWNDVAGL 135
           G  G AS  + A +     +    + G  E P++  L A L   ++   P V+W+DVAGL
Sbjct: 186 GKKGAASKSNKAESMNGDAEDGKSKRGLYEGPDED-LAAMLERDVLDSTPGVRWDDVAGL 245

Query: 136 ESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLAKAVATEADSTFFSIS 195
             AK+ L+EAV+LP+  P++F G RRPW+  L++GPPGTGK+ LAKAVATE  +TFF++S
Sbjct: 246 SEAKRLLEEAVVLPLWMPEYFQGIRRPWKGVLMFGPPGTGKTLLAKAVATECGTTFFNVS 305

Query: 196 SSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQRGEGNESEASRRIKTELL 255
           S+ L SKW GESE++V  LF++AR  APS IFIDEIDSLC  RG   E E+SRR+K+ELL
Sbjct: 306 SATLASKWRGESERMVRCLFDLARAYAPSTIFIDEIDSLCNSRGGSGEHESSRRVKSELL 365

Query: 256 VQMQGVGH-------NDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDAKARQHMFKV 315
           VQ+ GV +       + + V+VLAATN P+ +D+A+RRR +KRIYIPLPD ++R+ +  +
Sbjct: 366 VQVDGVSNTATNEDGSRKIVMVLAATNFPWDIDEALRRRLEKRIYIPLPDFESRKALINI 425

Query: 316 HLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMFFIKTPDGMWVP 375
           +L  T    ++ + E +ARRT+G+SG D++   +D     +R+                 
Sbjct: 426 NL-RTVEVASDVNIEDVARRTEGYSGDDLTNVCRDASMNGMRR----------------- 485

Query: 376 CGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLEIHEKFTKE 431
              K  G  +  ++ ++   +++     P+   DF++ + + +P+VS SD+E HEK+  E
Sbjct: 486 ---KIAGKTRDEIKNMSKDDISN----DPVAMCDFEEAIRKVQPSVSSSDIEKHEKWLSE 521

BLAST of CmoCh20G001800 vs. TAIR10
Match: AT2G34560.2 (AT2G34560.2 P-loop containing nucleoside triphosphate hydrolases superfamily protein)

HSP 1 Score: 256.5 bits (654), Expect = 2.9e-68
Identity = 146/365 (40.00%), Postives = 221/365 (60.55%), Query Frame = 1

Query: 72  VLDDGGPGPASNGDAAVATKP--KTKPKDGEGGDGEDPEQTKLRAGLNSAIIREKPDVKW 131
           V ++  P    +G+ A+A     + KPK       E  E   L   L+  IIR  P++KW
Sbjct: 52  VNNNSSPVTNQDGNTALANGNVIREKPKKSMFPPFESAETRTLAESLSRDIIRGNPNIKW 111

Query: 132 NDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLAKAVATEADS 191
             + GLE+AK+ L+EAV++P+K+P +F G   PW+  LL+GPPGTGK+ LAKAVATE ++
Sbjct: 112 ESIKGLENAKKLLKEAVVMPIKYPTYFNGLLTPWKGILLFGPPGTGKTMLAKAVATECNT 171

Query: 192 TFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQR-GEG-NESEAS 251
           TFF+IS+S +VSKW G+SEKL+  LF++AR  APS IF+DEID++  QR GEG +E EAS
Sbjct: 172 TFFNISASSVVSKWRGDSEKLIRVLFDLARHHAPSTIFLDEIDAIISQRGGEGRSEHEAS 231

Query: 252 RRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDAKARQHMFK 311
           RR+KTELL+QM G+   ++ V VLAATN P+ LD A+ RR +KRI +PLPD +AR+ MF+
Sbjct: 232 RRLKTELLIQMDGLQKTNELVFVLAATNLPWELDAAMLRRLEKRILVPLPDPEARRGMFE 291

Query: 312 VHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMFFIKTPDGMWV 371
           + +   P +      + L  +++G+SGSD+ +  K+   +P+R+T               
Sbjct: 292 MLIPSQPGD-EPLPHDVLVEKSEGYSGSDIRILCKEAAMQPLRRTL-------------- 351

Query: 372 PCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLEIHEKFTK 431
                   A+    +++  +    KI   PI   D D+ L+  RP+ +     +++KF  
Sbjct: 352 --------AILEDREDVVPEDELPKI--GPILPEDIDRALSNTRPS-AHLHAHLYDKFND 390

Query: 432 EFGEE 433
           ++G +
Sbjct: 412 DYGSQ 390

BLAST of CmoCh20G001800 vs. TAIR10
Match: AT2G45500.1 (AT2G45500.1 AAA-type ATPase family protein)

HSP 1 Score: 249.6 bits (636), Expect = 3.5e-66
Identity = 148/378 (39.15%), Postives = 213/378 (56.35%), Query Frame = 1

Query: 54  AITQKFTEYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQTKLR 113
           +++   + Y +   +   V   G   P +  DAA + KP       E G+  D +  ++ 
Sbjct: 152 SVSSTASRYRKTLSQKTPVARGGVATPRNPKDAAASPKPVK-----ESGNVYDDKLVEM- 211

Query: 114 AGLNSAIIREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPG 173
             +N+ I+   P VKW+DVAGL  AKQAL E VILP K    FTG RRP R  LL+GPPG
Sbjct: 212 --INTTIVDRSPSVKWDDVAGLNGAKQALLEMVILPAKRRDLFTGLRRPARGLLLFGPPG 271

Query: 174 TGKSYLAKAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDS 233
            GK+ LAKAVA+E+ +TFF++S+S L SKW+GE+EKLV  LF++A    PS+IF+DEIDS
Sbjct: 272 NGKTMLAKAVASESQATFFNVSASSLTSKWVGEAEKLVKTLFQVAISRQPSVIFMDEIDS 331

Query: 234 LCGQRGEGNESEASRRIKTELLVQMQGVGHN-DQKVLVLAATNTPYALDQAIRRRFDKRI 293
           +   R   +E+EASRR+K+E L+Q  GV  N D  V+++ ATN P  LD A+ RR  KRI
Sbjct: 332 IMSTRST-SENEASRRLKSEFLIQFDGVTSNPDDLVIIIGATNKPQELDDAVLRRLVKRI 391

Query: 294 YIPLPDAKARQHMFKVHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKT 353
           Y+PLPD+  R+ +FK  L   PH+L++ D + + + T+G+SGSD+    ++    P+R  
Sbjct: 392 YVPLPDSNVRKLLFKTKLKCQPHSLSDGDIDKIVKETEGYSGSDLQALCEEAAMMPIR-- 451

Query: 354 QDAMFFIKTPDGMWVPCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQRP 413
                               + GA  +T+Q    + L                 +A  RP
Sbjct: 452 --------------------ELGANILTIQANKVRSLRYDDFRKS---------MAVIRP 489

Query: 414 TVSKSDLEIHEKFTKEFG 431
           ++SKS  E  E++  EFG
Sbjct: 512 SLSKSKWEELERWNSEFG 489

BLAST of CmoCh20G001800 vs. TAIR10
Match: AT3G27120.1 (AT3G27120.1 P-loop containing nucleoside triphosphate hydrolases superfamily protein)

HSP 1 Score: 231.5 bits (589), Expect = 9.9e-61
Identity = 131/336 (38.99%), Postives = 192/336 (57.14%), Query Frame = 1

Query: 101 GGDGEDPE-----QTKLRAGLNSAIIREKPDVKWNDVAGLESAKQALQEAVILPVKFPQF 160
           G DGE PE     + +L   +++ I+   P+V+W+D+AGLE AK+ + E VI P+  P  
Sbjct: 165 GPDGELPEKLRNLEPRLIEHVSNEIMDRDPNVRWDDIAGLEHAKKCVTEMVIWPLLRPDI 224

Query: 161 FTGKRRPWRAFLLYGPPGTGKSYLAKAVATEADSTFFSISSSDLVSKWMGESEKLVSNLF 220
           F G R P +  LL+GPPGTGK+ + KA+A EA +TFF IS+S L SKW+GE EKLV  LF
Sbjct: 225 FKGCRSPGKGLLLFGPPGTGKTMIGKAIAGEAKATFFYISASSLTSKWIGEGEKLVRALF 284

Query: 221 EMARDSAPSIIFIDEIDSLCGQRGEGNESEASRRIKTELLVQMQGVGHNDQKVLVLAATN 280
            +A    P++IF+DEIDSL  QR    E E+SRR+KT+ L++M+G     +++L++ ATN
Sbjct: 285 GVASCRQPAVIFVDEIDSLLSQRKSDGEHESSRRLKTQFLIEMEGFDSGSEQILLIGATN 344

Query: 281 TPYALDQAIRRRFDKRIYIPLPDAKARQHMFKVHLG-DTPHNLTEADFESLARRTDGFSG 340
            P  LD+A RRR  KR+YIPLP ++AR  + +  L  D    L++ D   +   T+G+SG
Sbjct: 345 RPQELDEAARRRLTKRLYIPLPSSEARAWIIQNLLKKDGLFTLSDDDMNIICNLTEGYSG 404

Query: 341 SDVSVCVKDVLFEPVRKTQDAMFFIKTPDGMWVPCGPKQQGAVQITMQELAAKGLASKIL 400
           SD+   VKD    P+R+       I                   +T  ++          
Sbjct: 405 SDMKNLVKDATMGPLREALKRGIDI-----------------TNLTKDDMRL-------- 464

Query: 401 PPPITRTDFDKVLARQRPTVSKSDLEIHEKFTKEFG 431
              +T  DF   L   RP+VS+++L I+E +  +FG
Sbjct: 465 ---VTLQDFKDALQEVRPSVSQNELGIYENWNNQFG 472

BLAST of CmoCh20G001800 vs. NCBI nr
Match: gi|659117396|ref|XP_008458580.1| (PREDICTED: vacuolar protein sorting-associated protein 4 [Cucumis melo])

HSP 1 Score: 852.4 bits (2201), Expect = 3.4e-244
Identity = 425/433 (98.15%), Postives = 430/433 (99.31%), Query Frame = 1

Query: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60
           MYSNFKEQAIEYVKQAVHEDNAGNYAKAF LYMNALEYFKTHLKYEKNPKIKEAITQKFT
Sbjct: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKAFSLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60

Query: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQTKLRAGLNSAI 120
           EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQ KLRAGLNSAI
Sbjct: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQAKLRAGLNSAI 120

Query: 121 IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA 180
           IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA
Sbjct: 121 IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA 180

Query: 181 KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQRGE 240
           KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLF+MARDSAPSIIFIDEIDSLCGQRGE
Sbjct: 181 KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFQMARDSAPSIIFIDEIDSLCGQRGE 240

Query: 241 GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDAK 300
           GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPD K
Sbjct: 241 GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDLK 300

Query: 301 ARQHMFKVHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMFFIK 360
           ARQHMFKVHLGDTPHNLTEADFE+LARRTDGFSGSD+SVCVKDVLFEPVRKTQDAMFFIK
Sbjct: 301 ARQHMFKVHLGDTPHNLTEADFENLARRTDGFSGSDISVCVKDVLFEPVRKTQDAMFFIK 360

Query: 361 TPDGMWVPCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE 420
           TPDGMWVPCGPKQQGAVQI+MQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE
Sbjct: 361 TPDGMWVPCGPKQQGAVQISMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE 420

Query: 421 IHEKFTKEFGEEG 434
           IHE+FTKEFGEEG
Sbjct: 421 IHERFTKEFGEEG 433

BLAST of CmoCh20G001800 vs. NCBI nr
Match: gi|449463076|ref|XP_004149260.1| (PREDICTED: protein SUPPRESSOR OF K(+) TRANSPORT GROWTH DEFECT 1 [Cucumis sativus])

HSP 1 Score: 852.0 bits (2200), Expect = 4.4e-244
Identity = 424/433 (97.92%), Postives = 430/433 (99.31%), Query Frame = 1

Query: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60
           MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT
Sbjct: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60

Query: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQTKLRAGLNSAI 120
           EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQ KLRAGLNSAI
Sbjct: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQAKLRAGLNSAI 120

Query: 121 IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA 180
           IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA
Sbjct: 121 IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA 180

Query: 181 KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQRGE 240
           KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLF+MARDSAPSIIFIDEIDSLCGQRGE
Sbjct: 181 KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFQMARDSAPSIIFIDEIDSLCGQRGE 240

Query: 241 GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDAK 300
           GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPD K
Sbjct: 241 GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDLK 300

Query: 301 ARQHMFKVHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMFFIK 360
           ARQHMFKVHLGDTPHNLTEADFE+LAR+TDGFSGSD+SVCVKDVLFEPVRKTQDAMFFI 
Sbjct: 301 ARQHMFKVHLGDTPHNLTEADFENLARKTDGFSGSDISVCVKDVLFEPVRKTQDAMFFIM 360

Query: 361 TPDGMWVPCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE 420
           TPDGMWVPCGPKQQGAVQI+MQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE
Sbjct: 361 TPDGMWVPCGPKQQGAVQISMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE 420

Query: 421 IHEKFTKEFGEEG 434
           IHE+FTKEFGEEG
Sbjct: 421 IHERFTKEFGEEG 433

BLAST of CmoCh20G001800 vs. NCBI nr
Match: gi|544346004|dbj|BAN84248.1| (vacuolar protein sorting-associated protein 4-like [Cucumis sativus var. sativus])

HSP 1 Score: 847.4 bits (2188), Expect = 1.1e-242
Identity = 422/433 (97.46%), Postives = 429/433 (99.08%), Query Frame = 1

Query: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60
           MYSNFKEQAIEYVKQAVHEDNAGNYAKA PLY+NALEYFKTHLKYEKNPKIKEAITQKFT
Sbjct: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKASPLYINALEYFKTHLKYEKNPKIKEAITQKFT 60

Query: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQTKLRAGLNSAI 120
           EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQ KLRAGLNSAI
Sbjct: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQAKLRAGLNSAI 120

Query: 121 IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA 180
           IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA
Sbjct: 121 IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA 180

Query: 181 KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQRGE 240
           KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLF+MARDSAPSIIFIDEIDSLCGQRGE
Sbjct: 181 KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFQMARDSAPSIIFIDEIDSLCGQRGE 240

Query: 241 GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDAK 300
           GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPD K
Sbjct: 241 GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDLK 300

Query: 301 ARQHMFKVHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMFFIK 360
           ARQHMFKVHLGDTPHNLTEADFE+LAR+TDGFSGSD+SVCVKDVLFEPVRKTQDAMFFI 
Sbjct: 301 ARQHMFKVHLGDTPHNLTEADFENLARKTDGFSGSDISVCVKDVLFEPVRKTQDAMFFIM 360

Query: 361 TPDGMWVPCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE 420
           TPDGMWVPCGPKQQGAVQI+MQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE
Sbjct: 361 TPDGMWVPCGPKQQGAVQISMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE 420

Query: 421 IHEKFTKEFGEEG 434
           IHE+FTKEFGEEG
Sbjct: 421 IHERFTKEFGEEG 433

BLAST of CmoCh20G001800 vs. NCBI nr
Match: gi|657986582|ref|XP_008385424.1| (PREDICTED: vacuolar protein sorting-associated protein 4 [Malus domestica])

HSP 1 Score: 827.4 bits (2136), Expect = 1.2e-236
Identity = 409/433 (94.46%), Postives = 424/433 (97.92%), Query Frame = 1

Query: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60
           MYSNFKEQAIEYVKQAV EDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT
Sbjct: 1   MYSNFKEQAIEYVKQAVQEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60

Query: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQTKLRAGLNSAI 120
           EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQ KLRAGLNSAI
Sbjct: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGGDGEDPEQAKLRAGLNSAI 120

Query: 121 IREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA 180
           IREKP+V+WNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA
Sbjct: 121 IREKPNVQWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYLA 180

Query: 181 KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQRGE 240
           KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLF+MARDSAPSIIFIDEIDSLCG RGE
Sbjct: 181 KAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFQMARDSAPSIIFIDEIDSLCGTRGE 240

Query: 241 GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDAK 300
           GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPD K
Sbjct: 241 GNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDLK 300

Query: 301 ARQHMFKVHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMFFIK 360
           ARQHMFKVHLGDTPHNLTE+DFESLAR+TDGFSGSD++VCVKDVLFEPVRKTQDAMFF K
Sbjct: 301 ARQHMFKVHLGDTPHNLTESDFESLARKTDGFSGSDIAVCVKDVLFEPVRKTQDAMFFFK 360

Query: 361 TPDGMWVPCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDLE 420
            P  MW+PCGPKQ GAVQITMQ+LAAKGLAS+ILPPPIT+TDFDKVLARQRPTVSK+DLE
Sbjct: 361 DPKDMWIPCGPKQPGAVQITMQDLAAKGLASQILPPPITKTDFDKVLARQRPTVSKNDLE 420

Query: 421 IHEKFTKEFGEEG 434
           +HE+FT+EFGEEG
Sbjct: 421 VHERFTREFGEEG 433

BLAST of CmoCh20G001800 vs. NCBI nr
Match: gi|502083601|ref|XP_004487508.1| (PREDICTED: protein SUPPRESSOR OF K(+) TRANSPORT GROWTH DEFECT 1 isoform X1 [Cicer arietinum])

HSP 1 Score: 824.7 bits (2129), Expect = 7.5e-236
Identity = 409/434 (94.24%), Postives = 426/434 (98.16%), Query Frame = 1

Query: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIKEAITQKFT 60
           MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKI+EAITQKFT
Sbjct: 1   MYSNFKEQAIEYVKQAVHEDNAGNYAKAFPLYMNALEYFKTHLKYEKNPKIREAITQKFT 60

Query: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATKPKTKPKDGEGG-DGEDPEQTKLRAGLNSA 120
           EYLRRAEEIRAVLDDGGPGPASNGDAAVAT+PK+KPKDGEGG DGEDPEQ KLRAGLNSA
Sbjct: 61  EYLRRAEEIRAVLDDGGPGPASNGDAAVATRPKSKPKDGEGGGDGEDPEQAKLRAGLNSA 120

Query: 121 IIREKPDVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYL 180
           IIREKP+VKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYL
Sbjct: 121 IIREKPNVKWNDVAGLESAKQALQEAVILPVKFPQFFTGKRRPWRAFLLYGPPGTGKSYL 180

Query: 181 AKAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFEMARDSAPSIIFIDEIDSLCGQRG 240
           AKAVATEADSTFFSISSSDLVSKWMGESEKLVSNLF+MAR+SAPSIIFIDEIDSLCGQRG
Sbjct: 181 AKAVATEADSTFFSISSSDLVSKWMGESEKLVSNLFQMARESAPSIIFIDEIDSLCGQRG 240

Query: 241 EGNESEASRRIKTELLVQMQGVGHNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDA 300
           EGNESEASRRIKTELLVQMQGVG+NDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPD 
Sbjct: 241 EGNESEASRRIKTELLVQMQGVGNNDQKVLVLAATNTPYALDQAIRRRFDKRIYIPLPDL 300

Query: 301 KARQHMFKVHLGDTPHNLTEADFESLARRTDGFSGSDVSVCVKDVLFEPVRKTQDAMFFI 360
           KARQHMFKVHLGDTPHNLTE+DFE LA RT+GFSGSD+SVCVKDVLFEPVRKTQDAMFF 
Sbjct: 301 KARQHMFKVHLGDTPHNLTESDFEYLASRTEGFSGSDISVCVKDVLFEPVRKTQDAMFFF 360

Query: 361 KTPDGMWVPCGPKQQGAVQITMQELAAKGLASKILPPPITRTDFDKVLARQRPTVSKSDL 420
           K+P+GMW+PCGPKQQGAVQ TMQ+LA KGLASKILPPPITRTDF+KVLARQRPTVSKSDL
Sbjct: 361 KSPEGMWIPCGPKQQGAVQTTMQDLATKGLASKILPPPITRTDFEKVLARQRPTVSKSDL 420

Query: 421 EIHEKFTKEFGEEG 434
           E+HE+FTKEFGEEG
Sbjct: 421 EVHERFTKEFGEEG 434

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
VPS4_ARATH9.5e-23190.11Protein SUPPRESSOR OF K(+) TRANSPORT GROWTH DEFECT 1 OS=Arabidopsis thaliana GN=... [more]
VPS4_CANAL2.2e-12654.09Vacuolar protein sorting-associated protein 4 OS=Candida albicans (strain SC5314... [more]
VPS4B_HUMAN7.0e-12554.63Vacuolar protein sorting-associated protein 4B OS=Homo sapiens GN=VPS4B PE=1 SV=... [more]
VPS4B_BOVIN2.0e-12454.63Vacuolar protein sorting-associated protein 4B OS=Bos taurus GN=VPS4B PE=2 SV=1[more]
VPS4B_PONAB5.9e-12454.18Vacuolar protein sorting-associated protein 4B OS=Pongo abelii GN=VPS4B PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0KGS6_CUCSA3.1e-24497.92Uncharacterized protein OS=Cucumis sativus GN=Csa_6G152960 PE=3 SV=1[more]
T2HVY3_CUCSA3.1e-24497.92Vacuolar protein sorting-associated protein 4-like OS=Cucumis sativus var. sativ... [more]
T2HVB0_CUCSA7.5e-24397.46Vacuolar protein sorting-associated protein 4-like OS=Cucumis sativus var. sativ... [more]
I1LTU5_SOYBN2.0e-23593.58Uncharacterized protein OS=Glycine max GN=GLYMA_12G183700 PE=3 SV=1[more]
M5WHD1_PRUPE5.8e-23594.00Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005989mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G27600.15.4e-23290.11 AAA-type ATPase family protein[more]
AT1G80350.13.3e-7241.16 P-loop containing nucleoside triphosphate hydrolases superfamily pro... [more]
AT2G34560.22.9e-6840.00 P-loop containing nucleoside triphosphate hydrolases superfamily pro... [more]
AT2G45500.13.5e-6639.15 AAA-type ATPase family protein[more]
AT3G27120.19.9e-6138.99 P-loop containing nucleoside triphosphate hydrolases superfamily pro... [more]
Match NameE-valueIdentityDescription
gi|659117396|ref|XP_008458580.1|3.4e-24498.15PREDICTED: vacuolar protein sorting-associated protein 4 [Cucumis melo][more]
gi|449463076|ref|XP_004149260.1|4.4e-24497.92PREDICTED: protein SUPPRESSOR OF K(+) TRANSPORT GROWTH DEFECT 1 [Cucumis sativus... [more]
gi|544346004|dbj|BAN84248.1|1.1e-24297.46vacuolar protein sorting-associated protein 4-like [Cucumis sativus var. sativus... [more]
gi|657986582|ref|XP_008385424.1|1.2e-23694.46PREDICTED: vacuolar protein sorting-associated protein 4 [Malus domestica][more]
gi|502083601|ref|XP_004487508.1|7.5e-23694.24PREDICTED: protein SUPPRESSOR OF K(+) TRANSPORT GROWTH DEFECT 1 isoform X1 [Cice... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR003593AAA+_ATPase
IPR003959ATPase_AAA_core
IPR003960ATPase_AAA_CS
IPR007330MIT
IPR015415Vps4_C
IPR027417P-loop_NTPase
Vocabulary: Molecular Function
TermDefinition
GO:0005524ATP binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007032 endosome organization
biological_process GO:0006869 lipid transport
biological_process GO:0010091 trichome branching
biological_process GO:0007033 vacuole organization
biological_process GO:0016192 vesicle-mediated transport
biological_process GO:0008150 biological_process
biological_process GO:0006623 protein targeting to vacuole
biological_process GO:0016558 protein import into peroxisome matrix
biological_process GO:0010351 lithium ion transport
biological_process GO:0008152 metabolic process
biological_process GO:0016197 endosomal transport
biological_process GO:0006635 fatty acid beta-oxidation
biological_process GO:0006891 intra-Golgi vesicle-mediated transport
cellular_component GO:0005575 cellular_component
cellular_component GO:0005634 nucleus
cellular_component GO:0009506 plasmodesma
cellular_component GO:0005771 multivesicular body
molecular_function GO:0005524 ATP binding
molecular_function GO:0008568 microtubule-severing ATPase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G001800.1CmoCh20G001800.1mRNA


Analysis Name: InterPro Annotations of Cucurbita moschata
Date Performed: 2017-05-19
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003593AAA+ ATPase domainSMARTSM00382AAA_5coord: 162..298
score: 8.6
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 167..296
score: 9.5
IPR003960ATPase, AAA-type, conserved sitePROSITEPS00674AAAcoord: 268..287
scor
IPR007330MITGENE3DG3DSA:1.20.58.280coord: 2..75
score: 3.9
IPR007330MITPFAMPF04212MITcoord: 7..70
score: 5.3
IPR007330MITSMARTSM00745smartcoord: 2..79
score: 2.0
IPR007330MITunknownSSF116846MIT domaincoord: 2..81
score: 4.58
IPR015415Vps4 oligomerisation, C-terminalPFAMPF09336Vps4_Ccoord: 366..430
score: 1.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3DG3DSA:3.40.50.300coord: 121..304
score: 8.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseunknownSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 125..429
score: 1.55
NoneNo IPR availableGENE3DG3DSA:1.10.8.60coord: 305..412
score: 5.4
NoneNo IPR availablePANTHERPTHR23074AAA ATPASEcoord: 394..433
score: 0.0coord: 1..373
score:
NoneNo IPR availablePANTHERPTHR23074:SF93F10A12.27/F10A12.27coord: 394..433
score: 0.0coord: 1..373
score:

The following gene(s) are paralogous to this gene:

None