CmaCh04G019150 (gene) Cucurbita maxima (Rimu)

NameCmaCh04G019150
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionCalcium-binding EF-hand family protein, putative
LocationCma_Chr04 : 10198801 .. 10199259 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTATCCCCAAACTGCAAGCTTCCATGGCTAAGTATGAAGCAAGCGAGATGATCAAGAAGAATACTAGAAATGGTGATAATGTTCAACTAAAAGCTTTTAGTGGCATTACTCGAGCTTCAACTCAAGTCAACAATCAAGGCATGGTACCAGAAATTCTTAGAAATAATAAGAATGTCGGGCCCAAGCTTAGTTCAAATACTCATCTAAAGGCATCAAATATGCAATTTAGTCGAGAGCAGATGAAGAAGATCTTTAGATATCATGATAGCGACAGAGATGGTTTTCTCAATATAAGAGAGCTAACAAAGGCTTTTGCTTTTCTAGGCTCAATTATTCCATTTGAGAAAGCTTCCTATGGCATGACATATGCTGACACAAATAAAGATGGCCTTATCAACCAGGCTCAACTTGACAAACTAGTTGATTATGCCCAAAAATTTATGAAGAAGAAATGA

mRNA sequence

ATGTTTATCCCCAAACTGCAAGCTTCCATGGCTAAGTATGAAGCAAGCGAGATGATCAAGAAGAATACTAGAAATGGTGATAATGTTCAACTAAAAGCTTTTAGTGGCATTACTCGAGCTTCAACTCAAGTCAACAATCAAGGCATGGTACCAGAAATTCTTAGAAATAATAAGAATGTCGGGCCCAAGCTTAGTTCAAATACTCATCTAAAGGCATCAAATATGCAATTTAGTCGAGAGCAGATGAAGAAGATCTTTAGATATCATGATAGCGACAGAGATGGTTTTCTCAATATAAGAGAGCTAACAAAGGCTTTTGCTTTTCTAGGCTCAATTATTCCATTTGAGAAAGCTTCCTATGGCATGACATATGCTGACACAAATAAAGATGGCCTTATCAACCAGGCTCAACTTGACAAACTAGTTGATTATGCCCAAAAATTTATGAAGAAGAAATGA

Coding sequence (CDS)

ATGTTTATCCCCAAACTGCAAGCTTCCATGGCTAAGTATGAAGCAAGCGAGATGATCAAGAAGAATACTAGAAATGGTGATAATGTTCAACTAAAAGCTTTTAGTGGCATTACTCGAGCTTCAACTCAAGTCAACAATCAAGGCATGGTACCAGAAATTCTTAGAAATAATAAGAATGTCGGGCCCAAGCTTAGTTCAAATACTCATCTAAAGGCATCAAATATGCAATTTAGTCGAGAGCAGATGAAGAAGATCTTTAGATATCATGATAGCGACAGAGATGGTTTTCTCAATATAAGAGAGCTAACAAAGGCTTTTGCTTTTCTAGGCTCAATTATTCCATTTGAGAAAGCTTCCTATGGCATGACATATGCTGACACAAATAAAGATGGCCTTATCAACCAGGCTCAACTTGACAAACTAGTTGATTATGCCCAAAAATTTATGAAGAAGAAATGA

Protein sequence

MFIPKLQASMAKYEASEMIKKNTRNGDNVQLKAFSGITRASTQVNNQGMVPEILRNNKNVGPKLSSNTHLKASNMQFSREQMKKIFRYHDSDRDGFLNIRELTKAFAFLGSIIPFEKASYGMTYADTNKDGLINQAQLDKLVDYAQKFMKKK
BLAST of CmaCh04G019150 vs. TrEMBL
Match: A0A0A0KLF3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 2.4e-42
Identity = 100/173 (57.80%), Postives = 122/173 (70.52%), Query Frame = 1

Query: 1   MFIPKLQASMAKYEASEMIKKNTRNGDNVQLKAFSGITRASTQVN-NQGMVPEILRNNKN 60
           M IP+LQ S AKYEAS+++KK  +NG+ V L AF+   R STQ+N N+ M  + L+ N +
Sbjct: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDD 87

Query: 61  VGPKLSSNTHLK--------------------ASNMQFSREQMKKIFRYHDSDRDGFLNI 120
           V  KLSSN  LK                    ASNMQFSREQ+K+IF+Y+DSDRDGFLNI
Sbjct: 88  VAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNI 147

Query: 121 RELTKAFAFLGSIIPFEKASYGMTYADTNKDGLINQAQLDKLVDYAQKFMKKK 153
           RE+TKAFA LGSI PF KA +GM YADTNKDGLI + +LDKL++YA KFMKKK
Sbjct: 148 REVTKAFALLGSIFPFNKAYHGMVYADTNKDGLIGEDELDKLINYANKFMKKK 200

BLAST of CmaCh04G019150 vs. TrEMBL
Match: A0A0A0KIY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 7.2e-39
Identity = 94/176 (53.41%), Postives = 120/176 (68.18%), Query Frame = 1

Query: 1   MFIPKLQASMAKYEASEMIKKNTRNGDNVQLKAFSGITRASTQVNN-QGMVPEILRNNKN 60
           M+I  LQ S AKYEAS+++KK+  N + V LKAF+GI  AST++NN Q M  EI+    N
Sbjct: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60

Query: 61  --VGPKLSSNTHL---------------------KASNMQFSREQMKKIFRYHDSDRDGF 120
             V   L+SN ++                     KASNMQFSREQ+K+IF+YHDSD DGF
Sbjct: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120

Query: 121 LNIRELTKAFAFLGSIIPFEKASYGMTYADTNKDGLINQAQLDKLVDYAQKFMKKK 153
           LN+RE+TKAFAFLG+I+P  KA +GM YAD NKDGLI++ +L+KLVDYA KF  K+
Sbjct: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFNKR 176

BLAST of CmaCh04G019150 vs. TrEMBL
Match: A0A0A0KMQ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 1.6e-38
Identity = 91/172 (52.91%), Postives = 119/172 (69.19%), Query Frame = 1

Query: 1   MFIPKLQASMAKYEASEMIKKNTRNGDNVQLKAFSGITRASTQVNNQGMVPEILRNNKNV 60
           M+I KLQASMA YEAS +IK+  +NGD V++KAF  + R+STQ+ N+  V +IL+ +  V
Sbjct: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDV-RSSTQIGNRESVLKILKPSDGV 60

Query: 61  GPKLSSNTHL--------------------KASNMQFSREQMKKIFRYHDSDRDGFLNIR 120
             KL S  H+                    K+SNM  SRE++K+IF+YHD++ DGFLN  
Sbjct: 61  AHKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRM 120

Query: 121 ELTKAFAFLGSIIPFEKASYGMTYADTNKDGLINQAQLDKLVDYAQKFMKKK 153
           ELTKAFAFLGS+ PF KA YGM YAD N+DGLI++A+LDKL+DYA K +KKK
Sbjct: 121 ELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171

BLAST of CmaCh04G019150 vs. TrEMBL
Match: A0A0A0LRC8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.0e-32
Identity = 86/172 (50.00%), Postives = 117/172 (68.02%), Query Frame = 1

Query: 1   MFIPKLQASMAKYEASEMIKKNTRNGDNV----QLKAFSGITRASTQVNNQGMVPE--IL 60
           M +  LQA  AK EA+E+IKK  RNGD V    +L++F    R STQ+NN   V    + 
Sbjct: 1   MSVSHLQA-WAKNEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVP 60

Query: 61  RNNKNVGPKLSSNTHL--------------KASNMQFSREQMKKIFRYHDSDRDGFLNIR 120
           + N+N+  K+SSNT +              K SN+  SREQ+K+IF+YHDS++DGFL+IR
Sbjct: 61  KTNENLAQKVSSNTCITPKKQLLDKAQGSSKQSNVPLSREQIKEIFKYHDSNKDGFLSIR 120

Query: 121 ELTKAFAFLGSIIPFEKASYGMTYADTNKDGLINQAQLDKLVDYAQKFMKKK 153
           ELTKAF+ +GSI+PF KA Y M YAD ++DGL+++A+LDKL+DYA K +KKK
Sbjct: 121 ELTKAFSSMGSIVPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHKIIKKK 171

BLAST of CmaCh04G019150 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 4.8e-19
Identity = 48/80 (60.00%), Postives = 60/80 (75.00%), Query Frame = 1

Query: 73  SNMQFSREQMKKIFRYHDSDRDGFLNIRELTKAFAFLGSIIPFEKASYGMTYADTNKDGL 132
           S   FSRE+MK+IFR HD D DG+L+I EL KA  FLG  IPF KA YGM Y D N DGL
Sbjct: 7   SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 133 INQAQLDKLVDYAQKFMKKK 153
           I++ +LDKL+DYA++F K++
Sbjct: 67  ISEDELDKLIDYAERFQKRR 86

BLAST of CmaCh04G019150 vs. NCBI nr
Match: gi|700194512|gb|KGN49689.1| (hypothetical protein Csa_5G067670 [Cucumis sativus])

HSP 1 Score: 179.9 bits (455), Expect = 3.4e-42
Identity = 100/173 (57.80%), Postives = 122/173 (70.52%), Query Frame = 1

Query: 1   MFIPKLQASMAKYEASEMIKKNTRNGDNVQLKAFSGITRASTQVN-NQGMVPEILRNNKN 60
           M IP+LQ S AKYEAS+++KK  +NG+ V L AF+   R STQ+N N+ M  + L+ N +
Sbjct: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDD 87

Query: 61  VGPKLSSNTHLK--------------------ASNMQFSREQMKKIFRYHDSDRDGFLNI 120
           V  KLSSN  LK                    ASNMQFSREQ+K+IF+Y+DSDRDGFLNI
Sbjct: 88  VAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNI 147

Query: 121 RELTKAFAFLGSIIPFEKASYGMTYADTNKDGLINQAQLDKLVDYAQKFMKKK 153
           RE+TKAFA LGSI PF KA +GM YADTNKDGLI + +LDKL++YA KFMKKK
Sbjct: 148 REVTKAFALLGSIFPFNKAYHGMVYADTNKDGLIGEDELDKLINYANKFMKKK 200

BLAST of CmaCh04G019150 vs. NCBI nr
Match: gi|700194513|gb|KGN49690.1| (hypothetical protein Csa_5G068170 [Cucumis sativus])

HSP 1 Score: 168.3 bits (425), Expect = 1.0e-38
Identity = 94/176 (53.41%), Postives = 120/176 (68.18%), Query Frame = 1

Query: 1   MFIPKLQASMAKYEASEMIKKNTRNGDNVQLKAFSGITRASTQVNN-QGMVPEILRNNKN 60
           M+I  LQ S AKYEAS+++KK+  N + V LKAF+GI  AST++NN Q M  EI+    N
Sbjct: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60

Query: 61  --VGPKLSSNTHL---------------------KASNMQFSREQMKKIFRYHDSDRDGF 120
             V   L+SN ++                     KASNMQFSREQ+K+IF+YHDSD DGF
Sbjct: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120

Query: 121 LNIRELTKAFAFLGSIIPFEKASYGMTYADTNKDGLINQAQLDKLVDYAQKFMKKK 153
           LN+RE+TKAFAFLG+I+P  KA +GM YAD NKDGLI++ +L+KLVDYA KF  K+
Sbjct: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFNKR 176

BLAST of CmaCh04G019150 vs. NCBI nr
Match: gi|700194510|gb|KGN49687.1| (hypothetical protein Csa_5G067410 [Cucumis sativus])

HSP 1 Score: 167.2 bits (422), Expect = 2.3e-38
Identity = 91/172 (52.91%), Postives = 119/172 (69.19%), Query Frame = 1

Query: 1   MFIPKLQASMAKYEASEMIKKNTRNGDNVQLKAFSGITRASTQVNNQGMVPEILRNNKNV 60
           M+I KLQASMA YEAS +IK+  +NGD V++KAF  + R+STQ+ N+  V +IL+ +  V
Sbjct: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDV-RSSTQIGNRESVLKILKPSDGV 60

Query: 61  GPKLSSNTHL--------------------KASNMQFSREQMKKIFRYHDSDRDGFLNIR 120
             KL S  H+                    K+SNM  SRE++K+IF+YHD++ DGFLN  
Sbjct: 61  AHKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRM 120

Query: 121 ELTKAFAFLGSIIPFEKASYGMTYADTNKDGLINQAQLDKLVDYAQKFMKKK 153
           ELTKAFAFLGS+ PF KA YGM YAD N+DGLI++A+LDKL+DYA K +KKK
Sbjct: 121 ELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171

BLAST of CmaCh04G019150 vs. NCBI nr
Match: gi|449470190|ref|XP_004152801.1| (PREDICTED: probable calcium-binding protein CML11 [Cucumis sativus])

HSP 1 Score: 147.9 bits (372), Expect = 1.4e-32
Identity = 86/172 (50.00%), Postives = 117/172 (68.02%), Query Frame = 1

Query: 1   MFIPKLQASMAKYEASEMIKKNTRNGDNV----QLKAFSGITRASTQVNNQGMVPE--IL 60
           M +  LQA  AK EA+E+IKK  RNGD V    +L++F    R STQ+NN   V    + 
Sbjct: 1   MSVSHLQA-WAKNEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVP 60

Query: 61  RNNKNVGPKLSSNTHL--------------KASNMQFSREQMKKIFRYHDSDRDGFLNIR 120
           + N+N+  K+SSNT +              K SN+  SREQ+K+IF+YHDS++DGFL+IR
Sbjct: 61  KTNENLAQKVSSNTCITPKKQLLDKAQGSSKQSNVPLSREQIKEIFKYHDSNKDGFLSIR 120

Query: 121 ELTKAFAFLGSIIPFEKASYGMTYADTNKDGLINQAQLDKLVDYAQKFMKKK 153
           ELTKAF+ +GSI+PF KA Y M YAD ++DGL+++A+LDKL+DYA K +KKK
Sbjct: 121 ELTKAFSSMGSIVPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHKIIKKK 171

BLAST of CmaCh04G019150 vs. NCBI nr
Match: gi|659089539|ref|XP_008445564.1| (PREDICTED: probable calcium-binding protein CML11 [Cucumis melo])

HSP 1 Score: 137.9 bits (346), Expect = 1.5e-29
Identity = 82/168 (48.81%), Postives = 108/168 (64.29%), Query Frame = 1

Query: 1   MFIPKLQASMAKYEASEMIKKNTRNGDNVQLKAFSGITRASTQVNNQGMVPE--ILRNNK 60
           M +  LQA  AK EASE+IKK  RNGD V  K          ++ N   V    I + N+
Sbjct: 1   MSVSHLQA-WAKIEASELIKKYDRNGDGVLTKEELQSFLHDARLKNIRFVSSSTIPKTNE 60

Query: 61  NVGPKLSSNTHLKA--------------SNMQFSREQMKKIFRYHDSDRDGFLNIRELTK 120
           N+  K+SSNT +K               SN+  SREQ+K+IF+YHDS++DGFL+IRELTK
Sbjct: 61  NLDQKVSSNTRIKPKKQLLDKAQDSLRQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTK 120

Query: 121 AFAFLGSIIPFEKASYGMTYADTNKDGLINQAQLDKLVDYAQKFMKKK 153
           AF  LGSI+PF KA + M YAD ++DGLI++A+L KL+DYA+K +KKK
Sbjct: 121 AFGSLGSIVPFAKARHAMAYADVDEDGLISEAELHKLIDYAEKIIKKK 167

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0KLF3_CUCSA2.4e-4257.80Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1[more]
A0A0A0KIY4_CUCSA7.2e-3953.41Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1[more]
A0A0A0KMQ1_CUCSA1.6e-3852.91Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1[more]
A0A0A0LRC8_CUCSA1.0e-3250.00Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359950 PE=4 SV=1[more]
A0A0A0LKX1_CUCSA4.8e-1960.00Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
gi|700194512|gb|KGN49689.1|3.4e-4257.80hypothetical protein Csa_5G067670 [Cucumis sativus][more]
gi|700194513|gb|KGN49690.1|1.0e-3853.41hypothetical protein Csa_5G068170 [Cucumis sativus][more]
gi|700194510|gb|KGN49687.1|2.3e-3852.91hypothetical protein Csa_5G067410 [Cucumis sativus][more]
gi|449470190|ref|XP_004152801.1|1.4e-3250.00PREDICTED: probable calcium-binding protein CML11 [Cucumis sativus][more]
gi|659089539|ref|XP_008445564.1|1.5e-2948.81PREDICTED: probable calcium-binding protein CML11 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G019150.1CmaCh04G019150.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13405EF-hand_6coord: 82..109
score: 8.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 77..112
score: 12.338coord: 126..148
score: 5
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 9..142
score: 2.0
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 71..136
score: 1.43
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 90..102
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 73..145
score: 2.5
NoneNo IPR availablePANTHERPTHR10891:SF592CALCIUM-BINDING PROTEIN CML38-RELATEDcoord: 73..145
score: 2.5

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None