Cucsa.337650.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.337650.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Werner Syndrome-like exonuclease
Location	scaffold03356 : 691297 .. 691929 (+)
Sequence length	633

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCACTCTCACCATTTCCGACCGCCAAATCCCCCTCAACTCCCACGACTATTACGACATTACTTTCGACGACGATGAAATCCTCACTCTCGTCACTGCTTCCCCTTCCATGGTCGACATGTGGATCTCCGAAATCCTCCGTATTCACCGCCGTAGACTCCACCGTCTCGTCGTTGGTCTTGACGTTGAGTGGCGTCCTAACTTCGATCGTCATTTTCGTAACCCCGTCGCCACTTTACAACTCTGCGTCGGTCGCCGCTGCCTCATTTTCCAACTCATCCACGCATCTGAAACTCCTCAGTCTTTGATCGATTTCTTGGAGGATGATACTTTCACTTTTGTTGGCGTTGGAATCGACAATGACGTTCTCAAGCTCTACAATGATTACGATCTGAATGTTGCTAACACTGTTGATCTTAGGGAGTTGGCTGCGGATGAGATGCAGAGTGATGAGTTTAGGACTGCCGGATTGAAGACTTTGGGGAGAGAGGTTCTTGGAAGGGAGATTGATAAGCCGAGAAATGTGAAGTTAAGTCGGTGGGATCGTCAATGGCTGAACCCTGCTCAGATACTCTATGCTACTGTTGATGCATTTTTGTCGTTTGAGATTGGAAGGTATCTGCTGAGTTGA

mRNA sequence

ATGGCCACTCTCACCATTTCCGACCGCCAAATCCCCCTCAACTCCCACGACTATTACGACATTACTTTCGACGACGATGAAATCCTCACTCTCGTCACTGCTTCCCCTTCCATGGTCGACATGTGGATCTCCGAAATCCTCCGTATTCACCGCCGTAGACTCCACCGTCTCGTCGTTGGTCTTGACGTTGAGTGGCGTCCTAACTTCGATCGTCATTTTCGTAACCCCGTCGCCACTTTACAACTCTGCGTCGGTCGCCGCTGCCTCATTTTCCAACTCATCCACGCATCTGAAACTCCTCAGTCTTTGATCGATTTCTTGGAGGATGATACTTTCACTTTTGTTGGCGTTGGAATCGACAATGACGTTCTCAAGCTCTACAATGATTACGATCTGAATGTTGCTAACACTGTTGATCTTAGGGAGTTGGCTGCGGATGAGATGCAGAGTGATGAGTTTAGGACTGCCGGATTGAAGACTTTGGGGAGAGAGGTTCTTGGAAGGGAGATTGATAAGCCGAGAAATGTGAAGTTAAGTCGGTGGGATCGTCAATGGCTGAACCCTGCTCAGATACTCTATGCTACTGTTGATGCATTTTTGTCGTTTGAGATTGGAAGGTATCTGCTGAGTTGA

Coding sequence (CDS)

ATGGCCACTCTCACCATTTCCGACCGCCAAATCCCCCTCAACTCCCACGACTATTACGACATTACTTTCGACGACGATGAAATCCTCACTCTCGTCACTGCTTCCCCTTCCATGGTCGACATGTGGATCTCCGAAATCCTCCGTATTCACCGCCGTAGACTCCACCGTCTCGTCGTTGGTCTTGACGTTGAGTGGCGTCCTAACTTCGATCGTCATTTTCGTAACCCCGTCGCCACTTTACAACTCTGCGTCGGTCGCCGCTGCCTCATTTTCCAACTCATCCACGCATCTGAAACTCCTCAGTCTTTGATCGATTTCTTGGAGGATGATACTTTCACTTTTGTTGGCGTTGGAATCGACAATGACGTTCTCAAGCTCTACAATGATTACGATCTGAATGTTGCTAACACTGTTGATCTTAGGGAGTTGGCTGCGGATGAGATGCAGAGTGATGAGTTTAGGACTGCCGGATTGAAGACTTTGGGGAGAGAGGTTCTTGGAAGGGAGATTGATAAGCCGAGAAATGTGAAGTTAAGTCGGTGGGATCGTCAATGGCTGAACCCTGCTCAGATACTCTATGCTACTGTTGATGCATTTTTGTCGTTTGAGATTGGAAGGTATCTGCTGAGTTGA

Protein sequence

MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDNDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDRQWLNPAQILYATVDAFLSFEIGRYLLS*

Homology

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BLAST of Cucsa.337650.1 vs. Swiss-Prot
Match: WEX_ARATH (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana GN=WEX PE=1 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 1.3e-20
Identity = 59/170 (34.71%), Postives = 96/170 (56.47%), Query Frame = 1


Query: 41  MWISEILRIHRRRLHRLVVGLDVEWRPNFDRH-FRNPVATLQLCVGRR-CLIFQLIHASE 100
           M + ++L   R       VGLD+EWRP+F +      VAT+Q+CV    C +  + H S 
Sbjct: 112 MQLIKVLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFH-SG 171

Query: 101 TPQSLIDFLEDDTFTFVGVGIDNDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGL 160
            PQSL   +ED T   VG+GID D +KL++DY +++ +  DL +LA  ++  D  +  GL
Sbjct: 172 IPQSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGL 231

Query: 161 KTLGREVLGREIDKPRNVKLSRWDRQWLNPAQILYATVDAFLSFEIGRYL 209
            +L   ++ +E+ KP  ++L  W+   L+  Q+ YA  DA+ S+ + + L
Sbjct: 232 ASLTETLVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVL 278

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BLAST of Cucsa.337650.1 vs. Swiss-Prot
Match: WRN_HUMAN (Werner syndrome ATP-dependent helicase OS=Homo sapiens GN=WRN PE=1 SV=2)

HSP 1 Score: 94.4 bits (233), Expect = 1.6e-18
Identity = 55/152 (36.18%), Postives = 85/152 (55.92%), Query Frame = 1


Query: 58  VVGLDVEWRPNFDRHFRNPVATLQLCVGR-RCLIFQLIHASETPQSLIDFLEDDTFTFVG 117
           VVG D+EW P ++R     VA +QLCV   +C +F +   S  PQ L   LE+      G
Sbjct: 78  VVGFDMEWPPLYNRGKLGKVALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAG 137

Query: 118 VGIDNDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNV 177
           VGI+ D  KL  D+D+ + N V+L ++A  +++  E  T  L +L + +LG+++ K +++
Sbjct: 138 VGIEGDQWKLLRDFDIKLKNFVELTDVANKKLKCTE--TWSLNSLVKHLLGKQLLKDKSI 197

Query: 178 KLSRWDRQWLNPAQILYATVDAFLSFEIGRYL 209
           + S W +  L   Q LYA  DA+  F I R L
Sbjct: 198 RCSNWSKFPLTEDQKLYAATDAYAGFIIYRNL 227

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BLAST of Cucsa.337650.1 vs. Swiss-Prot
Match: WRN_XENLA (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis GN=wrn PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-14
Identity = 48/152 (31.58%), Postives = 81/152 (53.29%), Query Frame = 1


Query: 58  VVGLDVEWRPNFDRHFRNPVATLQLCVG-RRCLIFQLIHASETPQSLIDFLEDDTFTFVG 117
           V+G D+EW P + +     VA +Q+CV  ++C +F +   +  P+ L   LED++   VG
Sbjct: 72  VLGFDIEWPPVYTKGKTGKVALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVG 131

Query: 118 VGIDNDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNV 177
           VGI+ D  KL +DY+L +   ++L E+A  +++  E  T     L + +   ++ K ++ 
Sbjct: 132 VGIEGDQWKLMSDYELKLKGFIELSEMANQKLRCKEKWT--FNGLIKHLFKEQLYKRKSY 191

Query: 178 KLSRWDRQWLNPAQILYATVDAFLSFEIGRYL 209
           + S WD   L   Q LYA  DA+    I + L
Sbjct: 192 RCSNWDIFLLTEDQKLYAATDAYAGLLIYKKL 221

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BLAST of Cucsa.337650.1 vs. Swiss-Prot
Match: EXD2_HUMAN (Exonuclease 3'-5' domain-containing protein 2 OS=Homo sapiens GN=EXD2 PE=1 SV=2)

HSP 1 Score: 74.3 bits (181), Expect = 1.8e-12
Identity = 57/159 (35.85%), Postives = 82/159 (51.57%), Query Frame = 1


Query: 58  VVGLDVEWRPNFDRHFRNPVATLQ------LCVGRRCLIFQLIHASET-PQSLIDFLEDD 117
           V+G+D EW  N +    +P++ LQ      LCV  R  + +LI   +T P++L+D L D 
Sbjct: 104 VLGIDCEW-VNLEGK-ASPLSLLQMASPSGLCVLVR--LPKLICGGKTLPRTLLDILADG 163

Query: 118 TFTFVGVGIDNDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREI 177
           T   VGVG   D  KL  DY L V   +DLR LA  +  +       LK+L   VL   +
Sbjct: 164 TILKVGVGCSEDASKLLQDYGLVVRGCLDLRYLAMRQRNNLLCNGLSLKSLAETVLNFPL 223

Query: 178 DKPRNVKLSRWDRQWLNPAQILYATVDAFLSFEIGRYLL 210
           DK   ++ S WD + L   Q++YA  DA +S  +  +LL
Sbjct: 224 DKSLLLRCSNWDAETLTEDQVIYAARDAQISVALFLHLL 258

[Close]

BLAST of Cucsa.337650.1 vs. Swiss-Prot
Match: EXD2_MOUSE (Exonuclease 3'-5' domain-containing protein 2 OS=Mus musculus GN=Exd2 PE=1 SV=2)

HSP 1 Score: 69.7 bits (169), Expect = 4.3e-11
Identity = 59/191 (30.89%), Postives = 91/191 (47.64%), Query Frame = 1


Query: 25  DDEILTLVTASPSMVDMWISEILRIHRRRLHRL-VVGLDVEWRPNFDRHFRNPVATLQLC 84
           ++ IL     + S    W ++I    +R L    V+G+D EW  N +    +P++ LQ+ 
Sbjct: 100 EERILQAEVVTVSQEAEW-NQIQPFLKRELEDFPVLGIDCEW-VNLEGK-ASPLSLLQMA 159

Query: 85  VGRR-CLIFQL---IHASET-PQSLIDFLEDDTFTFVGVGIDNDVLKLYNDYDLNVANTV 144
                C + +L   I+   T P++L+D L D     VGVG   D  KL  DY L V   +
Sbjct: 160 SPSGFCALVRLPRLIYGGRTLPRTLLDILADGAILKVGVGCSEDANKLLQDYGLIVRGCL 219

Query: 145 DLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDRQWLNPAQILYATVDA 204
           DLR LA  +  +       LK+L   +L   +DK   ++ S WD + L   Q+ YA  DA
Sbjct: 220 DLRYLAMKQGNNILCNGLSLKSLAETILNFPLDKSLLLRCSNWDAENLTEDQVTYAARDA 279

Query: 205 FLSFEIGRYLL 210
            +S  +  +LL
Sbjct: 280 QISVALFLHLL 287

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BLAST of Cucsa.337650.1 vs. TrEMBL
Match: A0A0A0LHC5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G819830 PE=4 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 3.4e-116
Identity = 210/210 (100.00%), Postives = 210/210 (100.00%), Query Frame = 1


Query: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60
           MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG
Sbjct: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120
           LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID
Sbjct: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120

Query: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180
           NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR
Sbjct: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180

Query: 181 WDRQWLNPAQILYATVDAFLSFEIGRYLLS 211
           WDRQWLNPAQILYATVDAFLSFEIGRYLLS
Sbjct: 181 WDRQWLNPAQILYATVDAFLSFEIGRYLLS 210

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BLAST of Cucsa.337650.1 vs. TrEMBL
Match: A0A067EQM3_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g048832mg PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 5.6e-66
Identity = 128/205 (62.44%), Postives = 158/205 (77.07%), Query Frame = 1


Query: 4   LTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDV 63
           ++IS  ++  N+HD Y + F DD + T VT SPS+VD WISEI RIHRRRLH L+VGLDV
Sbjct: 3   ISISKHEVQYNTHDEYTVHFYDDVVFTQVTRSPSVVDDWISEIERIHRRRLHCLIVGLDV 62

Query: 64  EWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDNDV 123
           EWRP+F R  +NPVATLQLCVGRRCLIFQ+IHA   PQSL +FL D+ +TFVGVGID DV
Sbjct: 63  EWRPSFSRQ-QNPVATLQLCVGRRCLIFQIIHARRIPQSLANFLSDEDYTFVGVGIDGDV 122

Query: 124 LKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDR 183
            KL N+Y L V  TVDLR LAA++++ +  R AGLK L  EVL +E++KPRN+ LS WD 
Sbjct: 123 KKLENNYGLQVFRTVDLRPLAAEDLEIEGLRFAGLKALSWEVLEKEVNKPRNITLSAWDT 182

Query: 184 QWLNPAQILYATVDAFLSFEIGRYL 209
           + L PAQ+LYA +DAF++FEIGR L
Sbjct: 183 RVLTPAQVLYACLDAFIAFEIGRVL 206

[Close]

BLAST of Cucsa.337650.1 vs. TrEMBL
Match: V4UVL0_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10012747mg PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 5.6e-66
Identity = 128/205 (62.44%), Postives = 158/205 (77.07%), Query Frame = 1


Query: 4   LTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDV 63
           ++IS  ++  N+HD Y + F DD + T VT SPS+VD WISEI RIHRRRLH L+VGLDV
Sbjct: 3   ISISKHEVQYNTHDEYTVHFYDDVVFTQVTRSPSVVDDWISEIERIHRRRLHCLIVGLDV 62

Query: 64  EWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDNDV 123
           EWRP+F R  +NPVATLQLCVGRRCLIFQ+IHA   PQSL +FL D+ +TFVGVGID DV
Sbjct: 63  EWRPSFSRQ-QNPVATLQLCVGRRCLIFQIIHARRIPQSLANFLSDEDYTFVGVGIDGDV 122

Query: 124 LKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDR 183
            KL N+Y L V  TVDLR LAA++++ +  R AGLK L  EVL +E++KPRN+ LS WD 
Sbjct: 123 KKLENNYGLQVFRTVDLRPLAAEDLEIEGLRFAGLKALSWEVLEKEVNKPRNITLSAWDT 182

Query: 184 QWLNPAQILYATVDAFLSFEIGRYL 209
           + L PAQ+LYA +DAF++FEIGR L
Sbjct: 183 RVLTPAQVLYACLDAFIAFEIGRVL 206

[Close]

BLAST of Cucsa.337650.1 vs. TrEMBL
Match: A0A0A0LFG2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G819840 PE=4 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 3.1e-64
Identity = 125/207 (60.39%), Postives = 157/207 (75.85%), Query Frame = 1


Query: 4   LTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDV 63
           L I+D  IP  +HDYYDIT DDDEILTL TAS  +V+ W++ IL ++ RR+  L+VGLD+
Sbjct: 5   LNITDLHIPYYTHDYYDITIDDDEILTLRTASIDVVNFWVATILEVNNRRIRPLIVGLDI 64

Query: 64  EWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDNDV 123
           EWRP F     NPVATLQLCVG RCLIFQL++    PQ+L++FL D + TFVGVGI  DV
Sbjct: 65  EWRPYFGPK-PNPVATLQLCVGHRCLIFQLLYCPAAPQALVNFLFDSSCTFVGVGIHQDV 124

Query: 124 LKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDR 183
            KLY++Y L V+N VDLR+LA +++     R AGLK+L REVLGREI+KP+ + LS WD 
Sbjct: 125 QKLYHEYGLTVSNVVDLRDLAVNKLGRTYLRYAGLKSLWREVLGREIEKPKYITLSNWDS 184

Query: 184 QWLNPAQILYATVDAFLSFEIGRYLLS 211
            WLN AQILYAT+DAF+SFEIGR L++
Sbjct: 185 VWLNYAQILYATIDAFISFEIGRSLMN 210

[Close]

BLAST of Cucsa.337650.1 vs. TrEMBL
Match: A0A103YDM5_CYNCS (3'-5' exonuclease domain-containing protein OS=Cynara cardunculus var. scolymus GN=Ccrd_014481 PE=4 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 4.9e-62
Identity = 117/207 (56.52%), Postives = 158/207 (76.33%), Query Frame = 1


Query: 4   LTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDV 63
           ++I D ++P  +H+ YD+TF ++ I T+VT +PS VD WI+EI RIHRR LHRL+VGLDV
Sbjct: 3   MSIVDHELPDYTHNLYDVTFYENTISTIVTNTPSYVDSWIAEIERIHRRHLHRLIVGLDV 62

Query: 64  EWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDNDV 123
           EWRPN  R+  NPVA LQLCVG+RCL+FQ++H+   P+SL +FL + ++TF GVGI++DV
Sbjct: 63  EWRPNQSRNSDNPVAILQLCVGKRCLVFQILHSPTVPESLKNFLRNPSYTFAGVGIESDV 122

Query: 124 LKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDR 183
            KL  DY+L VAN  D+R LAA +    E + AGLK+L   VLG+EI KP+ V +SRWD 
Sbjct: 123 EKLTVDYNLVVANMADVRALAAVKYGVRELQNAGLKSLTMTVLGKEISKPKGVTMSRWDN 182

Query: 184 QWLNPAQILYATVDAFLSFEIGRYLLS 211
           +WL PAQ+ YA++DAFLSF+IGR ++S
Sbjct: 183 EWLTPAQVQYASIDAFLSFKIGRTVIS 209

[Close]

BLAST of Cucsa.337650.1 vs. TAIR10
Match: AT3G12430.1 (AT3G12430.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 101.7 bits (252), Expect = 5.8e-22
Identity = 67/209 (32.06%), Postives = 97/209 (46.41%), Query Frame = 1


Query: 19  YDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDVEWRPN---------- 78
           Y + F  +E + +VT  PS++  WI ++L  +R   H LVVG+ V+W P+          
Sbjct: 17  YLVDFFGEEFIVIVTPDPSVIGQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVR 76

Query: 79  ----------------FDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTF 138
                             R F  P  TLQLCVG RC+I QL H    PQ L +FL D  +
Sbjct: 77  YRSDSPPVRYRSDSPRVRRRFDPPADTLQLCVGNRCIIIQLSHCERVPQVLRNFLADRDY 136

Query: 139 TFVGVGIDNDVLKL-YNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGRE-I 198
           TFVG+    D  KL  + ++L +A  +DLR+  +D       +    + +  E LG   +
Sbjct: 137 TFVGIWNSQDAGKLKRSKHELEIAVLLDLRKFVSDS-SGRTMKRCSFEKIVEENLGHSGV 196

Query: 199 DKPRNVKLSRWDRQWLNPAQILYATVDAF 200
              R V  S W    L   QIL A++D +
Sbjct: 197 RLDRKVSRSDWRVYDLRYVQILQASIDVY 224

[Close]

BLAST of Cucsa.337650.1 vs. TAIR10
Match: AT4G13870.2 (AT4G13870.2 Werner syndrome-like exonuclease)

HSP 1 Score: 101.3 bits (251), Expect = 7.5e-22
Identity = 59/170 (34.71%), Postives = 96/170 (56.47%), Query Frame = 1


Query: 41  MWISEILRIHRRRLHRLVVGLDVEWRPNFDRH-FRNPVATLQLCVGRR-CLIFQLIHASE 100
           M + ++L   R       VGLD+EWRP+F +      VAT+Q+CV    C +  + H S 
Sbjct: 112 MQLIKVLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFH-SG 171

Query: 101 TPQSLIDFLEDDTFTFVGVGIDNDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGL 160
            PQSL   +ED T   VG+GID D +KL++DY +++ +  DL +LA  ++  D  +  GL
Sbjct: 172 IPQSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGGD--KKWGL 231

Query: 161 KTLGREVLGREIDKPRNVKLSRWDRQWLNPAQILYATVDAFLSFEIGRYL 209
            +L   ++ +E+ KP  ++L  W+   L+  Q+ YA  DA+ S+ + + L
Sbjct: 232 ASLTETLVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVL 278

[Close]

BLAST of Cucsa.337650.1 vs. TAIR10
Match: AT3G12410.1 (AT3G12410.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 97.4 bits (241), Expect = 1.1e-20
Identity = 67/211 (31.75%), Postives = 99/211 (46.92%), Query Frame = 1


Query: 14  NSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDVEW-------- 73
           N+H  Y + F  DE +  VT   S++  WI  +L  +R   H LVVG+ V+W        
Sbjct: 13  NTHLEYSVDFFGDEFIVTVTWDSSVISRWIRNVLFNNRFSSHPLVVGVGVQWTPFSYYSD 72

Query: 74  -RPN--------FDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVG 133
            RPN           +  NP   LQLCVG RCLI QL +  + P +L  FL D   TFVG
Sbjct: 73  PRPNNYYADPPPIRYYSDNPADILQLCVGNRCLIIQLGYCDQVPNNLRSFLADPETTFVG 132

Query: 134 VGIDNDVLKLYN-DYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGRE-IDKPR 193
           V    D  KL    + L +   +D+R    D       R +  + +  E +G + +    
Sbjct: 133 VWNGQDAGKLARCCHQLEIGELLDIRRYVTDSW-GRSMRRSSFEEIVEECMGYQGVMLDP 192

Query: 194 NVKLSRWDRQWLNPAQILYATVDAFLSFEIG 206
            + +S W    L+  QIL A++DA++  ++G
Sbjct: 193 EISMSDWTAYDLDLDQILQASLDAYVCHQLG 222

[Close]

BLAST of Cucsa.337650.1 vs. TAIR10
Match: AT2G36110.1 (AT2G36110.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 97.1 bits (240), Expect = 1.4e-20
Identity = 66/193 (34.20%), Postives = 96/193 (49.74%), Query Frame = 1


Query: 16  HDYYDITFDDDEILTLVTASPSMVDMWISEILRIHR-RRLHRLVVGLDVEWRPNFDRHFR 75
           H+   I F  + ++  VT + S +  WI  I    R R  H LVVGLDV+W P       
Sbjct: 14  HENRYIDFFGERLIVTVTHTTSTIRRWIHSIRFFSRLRSSHPLVVGLDVQWTPGGSDP-- 73

Query: 76  NPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDNDVLKLYN-DYDLN 135
            P   LQLCVG RCLI QL H    P+ L  FLED+T TFVGV    D  KL    + L 
Sbjct: 74  -PPDILQLCVGNRCLIIQLSHCKRIPEVLRSFLEDETITFVGVWNSQDQGKLERFRHQLE 133

Query: 136 VANTVDLRELAADEMQSDEFRTAGLKTLGREVLG-REIDKPRNVKLSRWDRQWLNPAQIL 195
           +   +D+R      + +  F     + +  E LG + + K + + +S W  + L+  QI+
Sbjct: 134 IWRLLDIRHYLPTRLLNSSF-----EKIVEECLGYKGVRKDKEICMSNWGARSLSHDQIV 193

Query: 196 YATVDAFLSFEIG 206
            A+ D ++  ++G
Sbjct: 194 QASDDVYVCCKLG 198

[Close]

BLAST of Cucsa.337650.1 vs. TAIR10
Match: AT3G12460.1 (AT3G12460.1 Polynucleotidyl transferase, ribonuclease H-like superfamily protein)

HSP 1 Score: 96.3 bits (238), Expect = 2.4e-20
Identity = 66/209 (31.58%), Postives = 94/209 (44.98%), Query Frame = 1


Query: 19  YDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDVEWRPNFDRHFRNPVA 78
           Y + F  +E+   VT  PS++  WI ++   +R   H LVVG+ V+W P+       PV+
Sbjct: 17  YSVDFFGEELFVTVTPDPSVIGQWIHDVFFHNRLSSHPLVVGVGVQWTPSGYHPASPPVS 76

Query: 79  --------------------------TLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTF 138
                                     TLQLCVG RC+I QL +    PQ L +FL D   
Sbjct: 77  YRSDSSPDSYRSDSPPVFYSSDPPADTLQLCVGNRCIIIQLRYCERVPQVLRNFLADRDN 136

Query: 139 TFVGVGIDNDVLKLYND-YDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGRE-I 198
           TFVG+    D  KL    + L +A  +DLRE  +D           L+ +  E LG   +
Sbjct: 137 TFVGIWNSQDAGKLERSRHQLEIAELMDLREFVSDSSGRRSMYNYSLEKIVEENLGYPGV 196

Query: 199 DKPRNVKLSRWDRQWLNPAQILYATVDAF 200
              R V +S W    L+  QIL A++D +
Sbjct: 197 RLDREVSMSDWRVYNLSYDQILQASIDVY 225

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BLAST of Cucsa.337650.1 vs. NCBI nr
Match: gi|449437986|ref|XP_004136771.1| (PREDICTED: Werner Syndrome-like exonuclease [Cucumis sativus])

HSP 1 Score: 425.6 bits (1093), Expect = 5.0e-116
Identity = 210/210 (100.00%), Postives = 210/210 (100.00%), Query Frame = 1


Query: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60
           MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG
Sbjct: 1   MATLTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVG 60

Query: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120
           LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID
Sbjct: 61  LDVEWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGID 120

Query: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180
           NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR
Sbjct: 121 NDVLKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSR 180

Query: 181 WDRQWLNPAQILYATVDAFLSFEIGRYLLS 211
           WDRQWLNPAQILYATVDAFLSFEIGRYLLS
Sbjct: 181 WDRQWLNPAQILYATVDAFLSFEIGRYLLS 210

[Close]

BLAST of Cucsa.337650.1 vs. NCBI nr
Match: gi|568856922|ref|XP_006482021.1| (PREDICTED: Werner Syndrome-like exonuclease [Citrus sinensis])

HSP 1 Score: 258.8 bits (660), Expect = 8.0e-66
Identity = 128/205 (62.44%), Postives = 158/205 (77.07%), Query Frame = 1


Query: 4   LTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDV 63
           ++IS  ++  N+HD Y + F DD + T VT SPS+VD WISEI RIHRRRLH L+VGLDV
Sbjct: 3   ISISKHEVQYNTHDEYTVHFYDDVVFTQVTRSPSVVDDWISEIERIHRRRLHCLIVGLDV 62

Query: 64  EWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDNDV 123
           EWRP+F R  +NPVATLQLCVGRRCLIFQ+IHA   PQSL +FL D+ +TFVGVGID DV
Sbjct: 63  EWRPSFSRQ-QNPVATLQLCVGRRCLIFQIIHARRIPQSLANFLSDEDYTFVGVGIDGDV 122

Query: 124 LKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDR 183
            KL N+Y L V  TVDLR LAA++++ +  R AGLK L  EVL +E++KPRN+ LS WD 
Sbjct: 123 KKLENNYGLQVFRTVDLRPLAAEDLEIEGLRFAGLKALSWEVLEKEVNKPRNITLSAWDT 182

Query: 184 QWLNPAQILYATVDAFLSFEIGRYL 209
           + L PAQ+LYA +DAF++FEIGR L
Sbjct: 183 RVLTPAQVLYACLDAFIAFEIGRVL 206

[Close]

BLAST of Cucsa.337650.1 vs. NCBI nr
Match: gi|567875789|ref|XP_006430484.1| (hypothetical protein CICLE_v10012747mg [Citrus clementina])

HSP 1 Score: 258.8 bits (660), Expect = 8.0e-66
Identity = 128/205 (62.44%), Postives = 158/205 (77.07%), Query Frame = 1


Query: 4   LTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDV 63
           ++IS  ++  N+HD Y + F DD + T VT SPS+VD WISEI RIHRRRLH L+VGLDV
Sbjct: 3   ISISKHEVQYNTHDEYTVHFYDDVVFTQVTRSPSVVDDWISEIERIHRRRLHCLIVGLDV 62

Query: 64  EWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDNDV 123
           EWRP+F R  +NPVATLQLCVGRRCLIFQ+IHA   PQSL +FL D+ +TFVGVGID DV
Sbjct: 63  EWRPSFSRQ-QNPVATLQLCVGRRCLIFQIIHARRIPQSLANFLSDEDYTFVGVGIDGDV 122

Query: 124 LKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDR 183
            KL N+Y L V  TVDLR LAA++++ +  R AGLK L  EVL +E++KPRN+ LS WD 
Sbjct: 123 KKLENNYGLQVFRTVDLRPLAAEDLEIEGLRFAGLKALSWEVLEKEVNKPRNITLSAWDT 182

Query: 184 QWLNPAQILYATVDAFLSFEIGRYL 209
           + L PAQ+LYA +DAF++FEIGR L
Sbjct: 183 RVLTPAQVLYACLDAFIAFEIGRVL 206

[Close]

BLAST of Cucsa.337650.1 vs. NCBI nr
Match: gi|449437988|ref|XP_004136772.1| (PREDICTED: Werner Syndrome-like exonuclease [Cucumis sativus])

HSP 1 Score: 253.1 bits (645), Expect = 4.4e-64
Identity = 125/207 (60.39%), Postives = 157/207 (75.85%), Query Frame = 1


Query: 4   LTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDV 63
           L I+D  IP  +HDYYDIT DDDEILTL TAS  +V+ W++ IL ++ RR+  L+VGLD+
Sbjct: 5   LNITDLHIPYYTHDYYDITIDDDEILTLRTASIDVVNFWVATILEVNNRRIRPLIVGLDI 64

Query: 64  EWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDNDV 123
           EWRP F     NPVATLQLCVG RCLIFQL++    PQ+L++FL D + TFVGVGI  DV
Sbjct: 65  EWRPYFGPK-PNPVATLQLCVGHRCLIFQLLYCPAAPQALVNFLFDSSCTFVGVGIHQDV 124

Query: 124 LKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDR 183
            KLY++Y L V+N VDLR+LA +++     R AGLK+L REVLGREI+KP+ + LS WD 
Sbjct: 125 QKLYHEYGLTVSNVVDLRDLAVNKLGRTYLRYAGLKSLWREVLGREIEKPKYITLSNWDS 184

Query: 184 QWLNPAQILYATVDAFLSFEIGRYLLS 211
            WLN AQILYAT+DAF+SFEIGR L++
Sbjct: 185 VWLNYAQILYATIDAFISFEIGRSLMN 210

[Close]

BLAST of Cucsa.337650.1 vs. NCBI nr
Match: gi|976922811|gb|KVI07167.1| (3'-5' exonuclease domain-containing protein [Cynara cardunculus var. scolymus])

HSP 1 Score: 245.7 bits (626), Expect = 7.0e-62
Identity = 117/207 (56.52%), Postives = 158/207 (76.33%), Query Frame = 1


Query: 4   LTISDRQIPLNSHDYYDITFDDDEILTLVTASPSMVDMWISEILRIHRRRLHRLVVGLDV 63
           ++I D ++P  +H+ YD+TF ++ I T+VT +PS VD WI+EI RIHRR LHRL+VGLDV
Sbjct: 3   MSIVDHELPDYTHNLYDVTFYENTISTIVTNTPSYVDSWIAEIERIHRRHLHRLIVGLDV 62

Query: 64  EWRPNFDRHFRNPVATLQLCVGRRCLIFQLIHASETPQSLIDFLEDDTFTFVGVGIDNDV 123
           EWRPN  R+  NPVA LQLCVG+RCL+FQ++H+   P+SL +FL + ++TF GVGI++DV
Sbjct: 63  EWRPNQSRNSDNPVAILQLCVGKRCLVFQILHSPTVPESLKNFLRNPSYTFAGVGIESDV 122

Query: 124 LKLYNDYDLNVANTVDLRELAADEMQSDEFRTAGLKTLGREVLGREIDKPRNVKLSRWDR 183
            KL  DY+L VAN  D+R LAA +    E + AGLK+L   VLG+EI KP+ V +SRWD 
Sbjct: 123 EKLTVDYNLVVANMADVRALAAVKYGVRELQNAGLKSLTMTVLGKEISKPKGVTMSRWDN 182

Query: 184 QWLNPAQILYATVDAFLSFEIGRYLLS 211
           +WL PAQ+ YA++DAFLSF+IGR ++S
Sbjct: 183 EWLTPAQVQYASIDAFLSFKIGRTVIS 209

The following BLAST results are available for this feature:

BLAST of Cucsa.337650.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
WEX_ARATH	1.3e-20	34.71	Werner Syndrome-like exonuclease OS=Arabidopsis thaliana GN=WEX PE=1 SV=1	[more]
WRN_HUMAN	1.6e-18	36.18	Werner syndrome ATP-dependent helicase OS=Homo sapiens GN=WRN PE=1 SV=2	[more]
WRN_XENLA	1.1e-14	31.58	Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis GN=wrn PE=2 SV=...	[more]
EXD2_HUMAN	1.8e-12	35.85	Exonuclease 3'-5' domain-containing protein 2 OS=Homo sapiens GN=EXD2 PE=1 SV=2	[more]
EXD2_MOUSE	4.3e-11	30.89	Exonuclease 3'-5' domain-containing protein 2 OS=Mus musculus GN=Exd2 PE=1 SV=2	[more]

BLAST of Cucsa.337650.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHC5_CUCSA	3.4e-116	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G819830 PE=4 SV=1	[more]
A0A067EQM3_CITSI	5.6e-66	62.44	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g048832mg PE=4 SV=1	[more]
V4UVL0_9ROSI	5.6e-66	62.44	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10012747mg PE=4 SV=1	[more]
A0A0A0LFG2_CUCSA	3.1e-64	60.39	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G819840 PE=4 SV=1	[more]
A0A103YDM5_CYNCS	4.9e-62	56.52	3'-5' exonuclease domain-containing protein OS=Cynara cardunculus var. scolymus ...	[more]

BLAST of Cucsa.337650.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G12430.1	5.8e-22	32.06	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G13870.2	7.5e-22	34.71	Werner syndrome-like exonuclease	[more]
AT3G12410.1	1.1e-20	31.75	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G36110.1	1.4e-20	34.20	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G12460.1	2.4e-20	31.58	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

BLAST of Cucsa.337650.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449437986\|ref\|XP_004136771.1\|	5.0e-116	100.00	PREDICTED: Werner Syndrome-like exonuclease [Cucumis sativus]	[more]
gi\|568856922\|ref\|XP_006482021.1\|	8.0e-66	62.44	PREDICTED: Werner Syndrome-like exonuclease [Citrus sinensis]	[more]
gi\|567875789\|ref\|XP_006430484.1\|	8.0e-66	62.44	hypothetical protein CICLE_v10012747mg [Citrus clementina]	[more]
gi\|449437988\|ref\|XP_004136772.1\|	4.4e-64	60.39	PREDICTED: Werner Syndrome-like exonuclease [Cucumis sativus]	[more]
gi\|976922811\|gb\|KVI07167.1\|	7.0e-62	56.52	3'-5' exonuclease domain-containing protein [Cynara cardunculus var. scolymus]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR002562	3'-5'_exonuclease_dom
IPR012337	RNaseH-like_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0003676	nucleic acid binding
GO:0008408	3'-5' exonuclease activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006139	nucleobase-containing compound metabolic process

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006139	nucleobase-containing compound metabolic process
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.337650	Cucsa.337650	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.337650.1	Cucsa.337650.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.337650.1.CDS.1	Cucsa.337650.1.CDS.1	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002562	3'-5' exonuclease domain	PFAM	PF01612	DNA_pol_A_exo1	coord: 50..208 score: 3.9
IPR012337	Ribonuclease H-like domain	GENE3D	G3DSA:3.30.420.10		coord: 16..209 score: 2.3
IPR012337	Ribonuclease H-like domain	unknown	SSF53098	Ribonuclease H-like	coord: 41..209 score: 2.58
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 1..210 score: 1.8