Cucsa.301840.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.301840.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Histidine triad nucleotide-binding protein 3
Location	scaffold02951 : 95861 .. 99371 (+)
Sequence length	429

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGCAGCAGCAGCAATTTCATCTTGTATTTTCTGCCAGAAAGCTTATAATTCTACCGCCAACAGCCTTCTTCATTTTGTCTGCTCCTTTCGTTTCTCTGATTAAAACCATTTTTCTTGTGCTTCTTCTTCTTCTTCTCTTATACCGGCGATTGAATGAAGCGTCTCTGATTAAGTATATCGGATTCTGCAGGATGAGAGAGTTGTTGCTTTTGAAGATATAAACCCTTCTGCTGTCAGGTATTTTGAACGGAACCTCTTAATCTATAGAATGAACCCTTTTTTCTAGCGTTTGATTCAGTTTTGCTATAGATATGAGGCTTCTGAGATATCTTCTGGGTTAATTTCATCTTCATTTTTTCATTGATTTAGCTTCTTTAGGAAAAAAaTCTTCTACTTTTGTATACCCATTTCGTCGGTTGCTTGATATTCAATGGACAATCTACATCTAGACCTGGATAGCAAATACCAATGGCGGCAGGAAAAATTTAGAAAGAGGCAATGTTTTAGATCTTTGGGCAAAAGTAATGCTAGACGGACTTATTGTTAAGGGTCTTTTACATTTTAAGTTCTGAACTGCCCCAAAATATTTCCAGATACAAGGTTGACAATGTACATTCTAGGATGTCATTATCAATTGAAGTCCTTGATAATCTGCTGAGTTCAAGAGCTCTTGTCGCTTATTTCGATCATACTTTTTTATGATTCTTCCACTTGTTGGTTTTTAACATTTATGACCTGTTTGTAGTAGGTTCTCAGTGGCCATCTACGACCTTCGTGAATTGCCATCTCGAAGGTGTTGAGGATGGCCTCTATAAGGCCACTAAATGGTGAGTGTCAATTGCCATTGTGGATTCTGTAAAATCTGGAAAGAGATATATGCATCACGTACTAAGAAACAAAGAAATAGTGGGTTCATTGTCATCTTTTGATTTGACTCTCATTTTTCAATCGTTTGTGTTTCTTCTGTTCGAGTCATTTACAAGAATTGGTCAACTCATTTTCAATCCTTTGATTTGTATTAGGATGCACTAGCAATTTCCACCACAAAAATGTTAGATGCAATTGATCTTCTGTTATTAAAGGCATCTATAAAACATTCTTACTTCGGATTGCCATTTTGTAGGCATTTCTTGGTAATTCCCAAGGAGCACATTCCCACAGTTAGGAATCTCCAGAGAAGAGCTGAAGATTACTCACTTGGTAATGTAACCATTGTTAAATGCAATTCATTTATGGTTTTTtGTTTAACGAAAGGGACAGGGGGAGGCAATGGGTGGGGGAACAAGTCCTTGAAAAGCAAGAGATATAGTTGCATGATATAATTGATAAAATCTATTGGTACCTTTTTGTCTGCATTAAACCTTACCATATTCTCTCCTTCCTTCGACCTCCATTTTTGAACCCTCTCATTCTCAAAGCTAGCCTTGGGGTGGGAAAAAAACTGCACGAAAATGAGTAGGCCATAATGCTCAACGTGGTCTTGACATTCCCGTTTTGAGAAAATAGTAAAGATTAGATATGCAAGCAAATTTCTTCCTTTCTTTCTGAAGGAAAAAAAaCCCAGGTGTTATATATGTGTCATGGATCTAGACTAGAGTCGCACCTTCTAAAACACAAGGGTTTTCTTTTGTTTGTTAAGAAACTGAACTTTCATTCAAAAAAATGAAAGAATACAATGGATTACAAAAAACAAGCCTGAAAAACATGAGTTCAACACTAACTACGAAAAGAGACTCAAATTCAAAGGAATAAGGCTGGTATCATAACTACAAAACGGTCTAGTGACCAGCTCCCAACCAGAAGCATTAAAGCTCGCAGACGTCCTCTCTAGATTTCTCCATATCCCTTAAAACTCTGCTATTAGGCTCCAACCAAGTACCCACAAAATAGCAAAGGAACTCTCTTGTCACAACATTTTTCCCTTCCTCGGGgAGCAAATGCAAAACCATCTTCTCCAACTAACAACACCCATCCCTGATACGAGCCCCACTAACCTCAAAAGAACTCCACCAACAATCCCAAAGGCAGTTTGTGAAATGACACTCCACAGTAAATGACTTAGATCCTCCTACTGCCTCCCACAGAGATTGCATTTTTAAATCTTTGGTCTGCTTGCTAATTCTGATAATGACTTGCTTATTTCTGTGTGGATTATTTTTAGCAGTAAGTCACATGTTGGAGGTGGGGCAGACACTTCTATCCCAAGATTCACCTCAATTAAAGCACAGGTACACTGAATGATAACCACTCTTCAAAACCATTATGTTGCTAGTCTTCAGCAAGTAAACGGTGTTTGTATTGTGTACTCTTCATTCAAATGCTAAAGCAGGGTAAAACTAAAGAAAACATGGATTTCAATGAATTAACAATGTCTCACATTCTCAATCCTTTCATATGTTTGGTTTATAATATCCCCACTTGGGCTGGTTTGTGCTTGCAACATATTATTGTGGAAACTTATAAAAATTGTTAATGTTTGCATTACCTTATGATAATTCATTGTGAACACTTCTATTAGTGTTGAAAAGCTACTTTAAGGGCATAGTACCTGATCGCAACTTAGTTCTTTCTTCCAAGGTGGACCAAGTGATGAATAATGTACTGTAATTGTGAATTCCTGCGAGTTTTGTTGTTCAAGTTCTACTACAAACTTTGTCTAGTGCGAAAGGATATCTTAGTTTCATTTCCTACATGGCATGATGGAGAATCAGAGATAGATCGCCTTTCTGTTCAGCAGAACGGATATAGGATCGATACCTGAAGAGAAGATTATCTTCTGGTCAATAGACTTTGGGCAAAGAAAAAGGAGTCTTGGTTCAGTTAATGATCAGGAGGTAGAAAGTGAGTTAACAAACTTGAATAAAAGAAATTTCACATCACATGTAAAGATAGTTTCCAGGCATTTCTTGGAATCTGATTCAGCATAACAGGTAATGAAAACTAGAGGCTTCTTTCCAAGGGGAAGGGGTGTGGATGACACTGAGAAACTTTGGCTCCAATGAAATTCCAGAGTTGAATTCATGAGTTGTTCTGATTTGGATTTCTTTGAAAGGTATTGATATAGGAATGACATTGGGACAAGATTAGGAATGTTATTAGGATTTTAAGAGTATATTAGTATTTAAACCTTCTTTTTACTACTGACATGAAATACTAAATGCTTCACAGTTCTGCTTTCAACTCTACTTTTAGTTCTTATATTTTACTTTAGAACAGCATGATTCTAAAATTGATGCTTCTCTTCTCTTTCTCTGCAAGATTTGGCTTCCATCAGCCACCGATGAACAGTGTCAATCACTTACATCTCCATTGTTTTGCTCTTCCTTACACACCCAGGTAACCAGTTTCTCAGTCTTATCAATGTGAAATTTGATACTATTTCTTTCAACAACAAAATTCTGAACCGAAACGGGGCAGCAAACAGGTGGAAGTTTGCAAAATATTTATCATTGGGATCGATCGGATTCATTGAAGCTGAGAAGTTGTTGGAGAAAaTAAAGCCTTAG

mRNA sequence

ATGGCAGCAGCAGCAGCAATTTCATCTTGTATTTTCTGCCAGAAAGCTTATAATTCTACCGCCAACAGCCTTCTTCATTTTGATGAGAGAGTTGTTGCTTTTGAAGATATAAACCCTTCTGCTGTCAGGCATTTCTTGGTAATTCCCAAGGAGCACATTCCCACAGTTAGGAATCTCCAGAGAAGAGCTGAAGATTACTCACTTGTAAGTCACATGTTGGAGGTGGGGCAGACACTTCTATCCCAAGATTCACCTCAATTAAAGCACAGATTTGGCTTCCATCAGCCACCGATGAACAGTGTCAATCACTTACATCTCCATTGTTTTGCTCTTCCTTACACACCCAGGTGGAAGTTTGCAAAATATTTATCATTGGGATCGATCGGATTCATTGAAGCTGAGAAGTTGTTGGAGAAAATAAAGCCTTAG

Coding sequence (CDS)

ATGGCAGCAGCAGCAGCAATTTCATCTTGTATTTTCTGCCAGAAAGCTTATAATTCTACCGCCAACAGCCTTCTTCATTTTGATGAGAGAGTTGTTGCTTTTGAAGATATAAACCCTTCTGCTGTCAGGCATTTCTTGGTAATTCCCAAGGAGCACATTCCCACAGTTAGGAATCTCCAGAGAAGAGCTGAAGATTACTCACTTGTAAGTCACATGTTGGAGGTGGGGCAGACACTTCTATCCCAAGATTCACCTCAATTAAAGCACAGATTTGGCTTCCATCAGCCACCGATGAACAGTGTCAATCACTTACATCTCCATTGTTTTGCTCTTCCTTACACACCCAGGTGGAAGTTTGCAAAATATTTATCATTGGGATCGATCGGATTCATTGAAGCTGAGAAGTTGTTGGAGAAAaTAAAGCCTTAG

Protein sequence

MAAAAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRAEDYSLVSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFAKYLSLGSIGFIEAEKLLEKIKP*

Homology

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BLAST of Cucsa.301840.1 vs. Swiss-Prot
Match: HINT4_ARATH (Bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4 OS=Arabidopsis thaliana GN=HINT4 PE=1 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 1.7e-51
Identity = 97/140 (69.29%), Postives = 110/140 (78.57%), Query Frame = 1


Query: 4   AAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRA 63
           A    +CIFC+   N T   LLH DE+V+AF+DI P+A RH+LVIPKEHIPTV +LQRR 
Sbjct: 2   AGVNQACIFCEIVRNPTTTRLLHTDEKVIAFQDIKPAAQRHYLVIPKEHIPTVNDLQRRD 61

Query: 64  EDYSLVSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFAKYL 123
           EDYSLV HML VGQ LL +D+PQ  HRFGFHQPP NSV+HLHLHCFALPY PRWK  KY 
Sbjct: 62  EDYSLVRHMLSVGQQLLQKDAPQSIHRFGFHQPPFNSVDHLHLHCFALPYVPRWKAIKYK 121

Query: 124 SLGSI-GFIEAEKLLEKIKP 143
           SLG + GFIEAE LLEKI+P
Sbjct: 122 SLGPLGGFIEAETLLEKIRP 141

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BLAST of Cucsa.301840.1 vs. Swiss-Prot
Match: HINT3_XENTR (Histidine triad nucleotide-binding protein 3 OS=Xenopus tropicalis GN=hint3 PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.4e-16
Identity = 48/136 (35.29%), Postives = 81/136 (59.56%), Query Frame = 1


Query: 9   SCIFCQKAYNSTANS-LLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRAEDYS 68
           SCIFC+ A    + + LLH D+ +V F+DI P+   H+LV+PK+H+ T + L +  +   
Sbjct: 18  SCIFCRIANKQESGAELLHSDDDLVCFKDIRPAVTHHYLVVPKKHVGTCKTLTK--DHVQ 77

Query: 69  LVSHMLEVGQTLLSQDS-PQLKH-RFGFHQPPMNSVNHLHLHCFALPYTPRWKFAKYLSL 128
           L+  M+EVG++ L +++   L+  R GFH PP  S++HLHLH  A      +       +
Sbjct: 78  LIKTMMEVGKSTLQKNNVTDLEDIRLGFHYPPFCSISHLHLHVLAPASQLGFLSRMIYRV 137

Query: 129 GSIGFIEAEKLLEKIK 142
            S  FI A++L+++++
Sbjct: 138 NSYWFITADELIDQLQ 151

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BLAST of Cucsa.301840.1 vs. Swiss-Prot
Match: HINT3_PONAB (Histidine triad nucleotide-binding protein 3 OS=Pongo abelii GN=HINT3 PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.4e-16
Identity = 53/139 (38.13%), Postives = 81/139 (58.27%), Query Frame = 1


Query: 8   SSCIFCQKA-YNSTANSLLHFD-ERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRAED 67
           S+C+FC+ A        LLH + E ++ F+DI P+A  H+LV+PK+HI   R L  R + 
Sbjct: 46  STCVFCRIAGRQDPGTELLHCENEDLICFKDIKPAATHHYLVVPKKHIGNCRTL--RKDQ 105

Query: 68  YSLVSHMLEVGQTLLSQD--SPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFAKYL 127
             LV +M+ VG+T+L ++  +     R GFH PP  S++HLHLH  A P       +K +
Sbjct: 106 VELVENMVTVGKTILERNNFTDFTNVRMGFHMPPFCSISHLHLHVLA-PVDQLGFLSKLV 165

Query: 128 -SLGSIGFIEAEKLLEKIK 142
             + S  FI A+ L+EK++
Sbjct: 166 YRVNSYWFITADHLIEKLR 181

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BLAST of Cucsa.301840.1 vs. Swiss-Prot
Match: HINT3_HUMAN (Histidine triad nucleotide-binding protein 3 OS=Homo sapiens GN=HINT3 PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.4e-16
Identity = 53/139 (38.13%), Postives = 81/139 (58.27%), Query Frame = 1


Query: 8   SSCIFCQKA-YNSTANSLLHFD-ERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRAED 67
           S+C+FC+ A        LLH + E ++ F+DI P+A  H+LV+PK+HI   R L  R + 
Sbjct: 46  STCVFCRIAGRQDPGTELLHCENEDLICFKDIKPAATHHYLVVPKKHIGNCRTL--RKDQ 105

Query: 68  YSLVSHMLEVGQTLLSQD--SPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFAKYL 127
             LV +M+ VG+T+L ++  +     R GFH PP  S++HLHLH  A P       +K +
Sbjct: 106 VELVENMVTVGKTILERNNFTDFTNVRMGFHMPPFCSISHLHLHVLA-PVDQLGFLSKLV 165

Query: 128 -SLGSIGFIEAEKLLEKIK 142
             + S  FI A+ L+EK++
Sbjct: 166 YRVNSYWFITADHLIEKLR 181

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BLAST of Cucsa.301840.1 vs. Swiss-Prot
Match: HINT3_RAT (Histidine triad nucleotide-binding protein 3 OS=Rattus norvegicus GN=Hint3 PE=2 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 3.7e-14
Identity = 47/138 (34.06%), Postives = 78/138 (56.52%), Query Frame = 1


Query: 8   SSCIFCQKAYNSTANSLLHF--DERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRAED 67
           S+C+FC+ A      + L +  ++ +V F+DI P+A+ H+LV+PK+HI + ++L +  + 
Sbjct: 39  SNCVFCRVAAGQEPETELLYCENKDLVCFKDIKPAALHHYLVVPKKHIGSCKDLNK--DH 98

Query: 68  YSLVSHMLEVGQTLLSQD--SPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFAKYL 127
             +V  M+ VG+T+L ++  +     R GFH PP  SV+HLHLH  A      +      
Sbjct: 99  IEMVESMVTVGKTILERNNFTDFTDVRMGFHVPPFCSVSHLHLHVIAPAKEFGFLSRVVY 158

Query: 128 SLGSIGFIEAEKLLEKIK 142
              S  FI  + LLEK++
Sbjct: 159 RRDSYWFITGDYLLEKLR 174

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BLAST of Cucsa.301840.1 vs. TrEMBL
Match: A0A0A0KKN0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G161990 PE=4 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 3.1e-76
Identity = 142/142 (100.00%), Postives = 142/142 (100.00%), Query Frame = 1


Query: 1   MAAAAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQ 60
           MAAAAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQ
Sbjct: 1   MAAAAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQ 60

Query: 61  RRAEDYSLVSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFA 120
           RRAEDYSLVSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFA
Sbjct: 61  RRAEDYSLVSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFA 120

Query: 121 KYLSLGSIGFIEAEKLLEKIKP 143
           KYLSLGSIGFIEAEKLLEKIKP
Sbjct: 121 KYLSLGSIGFIEAEKLLEKIKP 142

[Close]

BLAST of Cucsa.301840.1 vs. TrEMBL
Match: W9S6A5_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_011808 PE=4 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 2.3e-55
Identity = 105/141 (74.47%), Postives = 122/141 (86.52%), Query Frame = 1


Query: 2   AAAAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQR 61
           AAAA  + CIFCQ A  ST+ ++LH D++VVAF DINPSA RH+L+I   HIPTVR+LQR
Sbjct: 6   AAAAFQADCIFCQIASKSTSTTILHSDDKVVAFPDINPSAFRHYLIISVAHIPTVRDLQR 65

Query: 62  RAEDYSLVSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFAK 121
           +AEDY LVSHMLEVGQTLL++D+PQ K+RFGFHQPPMNSVNHLHLHC ALPYTPRWK  K
Sbjct: 66  KAEDYFLVSHMLEVGQTLLARDAPQCKYRFGFHQPPMNSVNHLHLHCQALPYTPRWKCMK 125

Query: 122 YLSLGSIGFIEAEKLLEKIKP 143
           YLS GS+GFIEA+KLLEK+KP
Sbjct: 126 YLSFGSLGFIEADKLLEKLKP 146

[Close]

BLAST of Cucsa.301840.1 vs. TrEMBL
Match: M5WVX2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012871mg PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 1.6e-53
Identity = 101/143 (70.63%), Postives = 123/143 (86.01%), Query Frame = 1


Query: 1   MAAAAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQ 60
           MAAAAA S CIFCQ A  ST+ +LLH D++VVAF+DI P+AVRH+LVIP +HIPTV++LQ
Sbjct: 1   MAAAAAASQCIFCQIASKSTSTTLLHTDDKVVAFQDIRPAAVRHYLVIPVDHIPTVKDLQ 60

Query: 61  RRAEDYSLVSHMLEVGQTLLSQDSPQL-KHRFGFHQPPMNSVNHLHLHCFALPYTPRWKF 120
           R+ EDYSLV+HMLEVG+ L+ +D+PQ  ++RFGFHQPP NSVNHLHLHCFALPYTPRWK 
Sbjct: 61  RKPEDYSLVNHMLEVGKMLVQRDAPQCHQYRFGFHQPPFNSVNHLHLHCFALPYTPRWKC 120

Query: 121 AKYLSLGSIGFIEAEKLLEKIKP 143
            KY+++G  GF+EAEKLL KIKP
Sbjct: 121 MKYIAMGPFGFLEAEKLLGKIKP 143

[Close]

BLAST of Cucsa.301840.1 vs. TrEMBL
Match: K4CIM5_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 2.8e-53
Identity = 101/139 (72.66%), Postives = 123/139 (88.49%), Query Frame = 1


Query: 6   AISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRAED 65
           A+S CIFCQ A +ST+ +LLH D++VVAF+DINPSA RH+LVIPK+HIPTV+NLQR ++D
Sbjct: 5   ALSECIFCQIATSSTSTTLLHSDDKVVAFQDINPSAFRHYLVIPKQHIPTVKNLQRSSDD 64

Query: 66  YSLVSHMLEVGQTLLSQDSPQLKH-RFGFHQPPMNSVNHLHLHCFALPYTPRWKFAKYLS 125
           +SLVSHML+VG++LL +D+PQ KH RFGFHQPP NSV+HLHLHCFALPYTP W+F KYLS
Sbjct: 65  FSLVSHMLDVGKSLLDRDAPQSKHYRFGFHQPPFNSVDHLHLHCFALPYTPSWRFMKYLS 124

Query: 126 LGSI-GFIEAEKLLEKIKP 143
           LG + GFIE EKLLE+IKP
Sbjct: 125 LGPLGGFIEVEKLLERIKP 143

[Close]

BLAST of Cucsa.301840.1 vs. TrEMBL
Match: A0A0V0GW67_SOLCH (Putative histidine triad nucleotide-binding protein 3-like OS=Solanum chacoense PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 9.1e-52
Identity = 99/139 (71.22%), Postives = 121/139 (87.05%), Query Frame = 1


Query: 6   AISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRAED 65
           A+S CIFCQ A +ST+ +LLH D++VVAF DINPSA RH+LVIPK+HIPTV++LQR ++D
Sbjct: 15  ALSDCIFCQIATSSTSTTLLHSDDKVVAFRDINPSAFRHYLVIPKQHIPTVKSLQRSSDD 74

Query: 66  YSLVSHMLEVGQTLLSQDSPQLKH-RFGFHQPPMNSVNHLHLHCFALPYTPRWKFAKYLS 125
           +SLVSHML+VG++LL +D+PQ KH RFGFHQPP NSV+HLHLHCFALPYTP W+F KYLS
Sbjct: 75  FSLVSHMLDVGKSLLHRDAPQSKHYRFGFHQPPFNSVDHLHLHCFALPYTPSWRFMKYLS 134

Query: 126 LGSI-GFIEAEKLLEKIKP 143
           LG + GFIE EKLLE+I P
Sbjct: 135 LGPLGGFIEVEKLLERINP 153

[Close]

BLAST of Cucsa.301840.1 vs. TAIR10
Match: AT4G16566.1 (AT4G16566.1 histidine triad nucleotide-binding 4)

HSP 1 Score: 203.4 bits (516), Expect = 9.6e-53
Identity = 97/140 (69.29%), Postives = 110/140 (78.57%), Query Frame = 1


Query: 4   AAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRRA 63
           A    +CIFC+   N T   LLH DE+V+AF+DI P+A RH+LVIPKEHIPTV +LQRR 
Sbjct: 2   AGVNQACIFCEIVRNPTTTRLLHTDEKVIAFQDIKPAAQRHYLVIPKEHIPTVNDLQRRD 61

Query: 64  EDYSLVSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFAKYL 123
           EDYSLV HML VGQ LL +D+PQ  HRFGFHQPP NSV+HLHLHCFALPY PRWK  KY 
Sbjct: 62  EDYSLVRHMLSVGQQLLQKDAPQSIHRFGFHQPPFNSVDHLHLHCFALPYVPRWKAIKYK 121

Query: 124 SLGSI-GFIEAEKLLEKIKP 143
           SLG + GFIEAE LLEKI+P
Sbjct: 122 SLGPLGGFIEAETLLEKIRP 141

[Close]

BLAST of Cucsa.301840.1 vs. NCBI nr
Match: gi|449469392|ref|XP_004152404.1| (PREDICTED: histidine triad nucleotide-binding protein 3 isoform X2 [Cucumis sativus])

HSP 1 Score: 292.4 bits (747), Expect = 4.4e-76
Identity = 142/142 (100.00%), Postives = 142/142 (100.00%), Query Frame = 1


Query: 1   MAAAAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQ 60
           MAAAAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQ
Sbjct: 1   MAAAAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQ 60

Query: 61  RRAEDYSLVSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFA 120
           RRAEDYSLVSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFA
Sbjct: 61  RRAEDYSLVSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFA 120

Query: 121 KYLSLGSIGFIEAEKLLEKIKP 143
           KYLSLGSIGFIEAEKLLEKIKP
Sbjct: 121 KYLSLGSIGFIEAEKLLEKIKP 142

[Close]

BLAST of Cucsa.301840.1 vs. NCBI nr
Match: gi|778700040|ref|XP_011654800.1| (PREDICTED: histidine triad nucleotide-binding protein 3 isoform X1 [Cucumis sativus])

HSP 1 Score: 287.7 bits (735), Expect = 1.1e-74
Identity = 142/143 (99.30%), Postives = 142/143 (99.30%), Query Frame = 1


Query: 1   MAAAAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQ 60
           MAAAAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQ
Sbjct: 1   MAAAAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQ 60

Query: 61  RRAEDYSL-VSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKF 120
           RRAEDYSL VSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKF
Sbjct: 61  RRAEDYSLAVSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKF 120

Query: 121 AKYLSLGSIGFIEAEKLLEKIKP 143
           AKYLSLGSIGFIEAEKLLEKIKP
Sbjct: 121 AKYLSLGSIGFIEAEKLLEKIKP 143

[Close]

BLAST of Cucsa.301840.1 vs. NCBI nr
Match: gi|659073483|ref|XP_008437084.1| (PREDICTED: histidine triad nucleotide-binding protein 3 isoform X2 [Cucumis melo])

HSP 1 Score: 275.0 bits (702), Expect = 7.3e-71
Identity = 133/140 (95.00%), Postives = 134/140 (95.71%), Query Frame = 1


Query: 3   AAAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRR 62
           AA  ISSCIFCQKA NSTAN+LLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRR
Sbjct: 2   AATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRR 61

Query: 63  AEDYSLVSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFAKY 122
           AEDYSLVSHML VGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPY PRWKF KY
Sbjct: 62  AEDYSLVSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVKY 121

Query: 123 LSLGSIGFIEAEKLLEKIKP 143
           LSLGSIGFIEAEKLLEKIKP
Sbjct: 122 LSLGSIGFIEAEKLLEKIKP 141

[Close]

BLAST of Cucsa.301840.1 vs. NCBI nr
Match: gi|659073481|ref|XP_008437083.1| (PREDICTED: histidine triad nucleotide-binding protein 3 isoform X1 [Cucumis melo])

HSP 1 Score: 270.4 bits (690), Expect = 1.8e-69
Identity = 133/141 (94.33%), Postives = 134/141 (95.04%), Query Frame = 1


Query: 3   AAAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRR 62
           AA  ISSCIFCQKA NSTAN+LLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRR
Sbjct: 2   AATTISSCIFCQKASNSTANNLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQRR 61

Query: 63  AEDYSL-VSHMLEVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFAK 122
           AEDYSL VSHML VGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPY PRWKF K
Sbjct: 62  AEDYSLAVSHMLGVGQTLLSQDSPQLKHRFGFHQPPMNSVNHLHLHCFALPYAPRWKFVK 121

Query: 123 YLSLGSIGFIEAEKLLEKIKP 143
           YLSLGSIGFIEAEKLLEKIKP
Sbjct: 122 YLSLGSIGFIEAEKLLEKIKP 142

[Close]

BLAST of Cucsa.301840.1 vs. NCBI nr
Match: gi|657955964|ref|XP_008369457.1| (PREDICTED: histidine triad nucleotide-binding protein 3 isoform X1 [Malus domestica])

HSP 1 Score: 224.9 bits (572), Expect = 8.7e-56
Identity = 107/142 (75.35%), Postives = 124/142 (87.32%), Query Frame = 1


Query: 2   AAAAAISSCIFCQKAYNSTANSLLHFDERVVAFEDINPSAVRHFLVIPKEHIPTVRNLQR 61
           A AAA S CIFCQ A  ST+ +L H DE+VVAF+DINP+AVRH+LVIP +HIPTV++LQR
Sbjct: 3   AVAAAASPCIFCQIAGKSTSTTLFHTDEKVVAFQDINPAAVRHYLVIPVDHIPTVKDLQR 62

Query: 62  RAEDYSLVSHMLEVGQTLLSQDSPQL-KHRFGFHQPPMNSVNHLHLHCFALPYTPRWKFA 121
           R EDYSLVSHMLEVG+TL+ +D+PQ  ++RFGFHQPP NSVNHLHLHCFALPYTPRWK  
Sbjct: 63  RPEDYSLVSHMLEVGKTLIQRDAPQCHQYRFGFHQPPFNSVNHLHLHCFALPYTPRWKCI 122

Query: 122 KYLSLGSIGFIEAEKLLEKIKP 143
           KYLS+GSIGF+EAEKLL KIKP
Sbjct: 123 KYLSVGSIGFVEAEKLLGKIKP 144

The following BLAST results are available for this feature:

BLAST of Cucsa.301840.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HINT4_ARATH	1.7e-51	69.29	Bifunctional adenosine 5'-phosphosulfate phosphorylase/adenylylsulfatase HINT4 O...	[more]
HINT3_XENTR	1.4e-16	35.29	Histidine triad nucleotide-binding protein 3 OS=Xenopus tropicalis GN=hint3 PE=2...	[more]
HINT3_PONAB	1.4e-16	38.13	Histidine triad nucleotide-binding protein 3 OS=Pongo abelii GN=HINT3 PE=2 SV=1	[more]
HINT3_HUMAN	1.4e-16	38.13	Histidine triad nucleotide-binding protein 3 OS=Homo sapiens GN=HINT3 PE=1 SV=1	[more]
HINT3_RAT	3.7e-14	34.06	Histidine triad nucleotide-binding protein 3 OS=Rattus norvegicus GN=Hint3 PE=2 ...	[more]

BLAST of Cucsa.301840.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KKN0_CUCSA	3.1e-76	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G161990 PE=4 SV=1	[more]
W9S6A5_9ROSA	2.3e-55	74.47	Uncharacterized protein OS=Morus notabilis GN=L484_011808 PE=4 SV=1	[more]
M5WVX2_PRUPE	1.6e-53	70.63	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012871mg PE=4 SV=1	[more]
K4CIM5_SOLLC	2.8e-53	72.66	Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1	[more]
A0A0V0GW67_SOLCH	9.1e-52	71.22	Putative histidine triad nucleotide-binding protein 3-like OS=Solanum chacoense ...	[more]

BLAST of Cucsa.301840.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G16566.1	9.6e-53	69.29	histidine triad nucleotide-binding 4	[more]

BLAST of Cucsa.301840.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449469392\|ref\|XP_004152404.1\|	4.4e-76	100.00	PREDICTED: histidine triad nucleotide-binding protein 3 isoform X2 [Cucumis sati...	[more]
gi\|778700040\|ref\|XP_011654800.1\|	1.1e-74	99.30	PREDICTED: histidine triad nucleotide-binding protein 3 isoform X1 [Cucumis sati...	[more]
gi\|659073483\|ref\|XP_008437084.1\|	7.3e-71	95.00	PREDICTED: histidine triad nucleotide-binding protein 3 isoform X2 [Cucumis melo...	[more]
gi\|659073481\|ref\|XP_008437083.1\|	1.8e-69	94.33	PREDICTED: histidine triad nucleotide-binding protein 3 isoform X1 [Cucumis melo...	[more]
gi\|657955964\|ref\|XP_008369457.1\|	8.7e-56	75.35	PREDICTED: histidine triad nucleotide-binding protein 3 isoform X1 [Malus domest...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001310	Histidine_triad_HIT
IPR011146	HIT-like

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009150	purine ribonucleotide metabolic process
biological_process	GO:0006790	sulfur compound metabolic process
cellular_component	GO:0005777	peroxisome
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0047627	adenylylsulfatase activity
molecular_function	GO:0004780	sulfate adenylyltransferase (ADP) activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.301840	Cucsa.301840	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.301840.1	Cucsa.301840.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.301840.1.CDS.1	Cucsa.301840.1.CDS.1	CDS
Cucsa.301840.1.CDS.2	Cucsa.301840.1.CDS.2	CDS
Cucsa.301840.1.CDS.3	Cucsa.301840.1.CDS.3	CDS
Cucsa.301840.1.CDS.4	Cucsa.301840.1.CDS.4	CDS
Cucsa.301840.1.CDS.5	Cucsa.301840.1.CDS.5	CDS
Cucsa.301840.1.CDS.6	Cucsa.301840.1.CDS.6	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001310	Histidine triad (HIT) protein	PRINTS	PR00332	HISTRIAD	coord: 99..109 score: 3.8E-7coord: 32..50 score: 3.
IPR011146	HIT-like domain	GENE3D	G3DSA:3.30.428.10		coord: 8..109 score: 1.2
IPR011146	HIT-like domain	PROFILE	PS51084	HIT_2	coord: 11..119 score: 12
IPR011146	HIT-like domain	unknown	SSF54197	HIT-like	coord: 7..130 score: 2.32
None	No IPR available	PANTHER	PTHR12486	APRATAXIN-RELATED	coord: 1..141 score: 5.2
None	No IPR available	PFAM	PF11969	DcpS_C	coord: 9..117 score: 2.0