Cucsa.162860.2 (mRNA) Cucumber (Gy14) v1

NameCucsa.162860.2
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionMajor facilitator superfamily protein
Locationscaffold01144 : 2376303 .. 2379770 (+)
Sequence length1317
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTACAGCCATTCCGGTGGCTTAAAATGCTTTCAACGGAGCTACATCCAAGTTTCATCGCCGGAGTAATCCTCATCTACGGTCTAAGCCACGGCTTCTCCGGCTCTTATTTCAAAGTCGTTTCCGATTACTACTGGAAAGACGTACAAAAGCTACAACCCTCAGTAGTTCAGATCTACATCGGGATATACACGATTCCGTGGGTGATGAAACCAATTTGGGGGTTATTAACGGACGCGTTTCCAGTGAGAGGGTATTTGCGGCGGCCGTATTTCGTGATTTCGGGGGTGGTTGGaGCAACGGCGGCGGTTGGGATGGCAGTgAAAGGGGGATTGGGGGTTTTGGAAGCATTGGGGCTTTTGATTGGGATTTCAGGGGCGATGGCGATTGCGGATGTGACGATTGATGCTTGCATTGTGAGAAATAGCATTGAAGTTCAGTGGTTAGCCCAAGATTTGCAAAGCTTGTGTGGAGCTTGTTCTTCGATTGGGCATTTGATTGGATATTCAACTAGTGGATTTTTTGTTCATCTTCTTGGCGCTCAGGTCCTTAATCTAATACCCTCTCTTTATTTTCCTTTTtCTAAATAATATTTCACCCATCTCTATGCATCTTCTTCTTTTCTTATAATTATAATAATAACAATTTAAAATAAAATTAACAAAAACTAAGGACAGAAATGTATATGTATAATAAACTAATAAATCATCAATCATTTAATATAAATTAACCATACACGGTTAGGTAGTGCCTAAAATCATAAAAATCATATTCCTGTTTTCTTTTCACAAATTTACTTTTTTtAAATACTAGCCTGTTAAGGTGACAAACAATAAAAGAAGGAATTCAAGTGTGATGTAGGATTTTATTTCAACAAATTTCTAATACTGTAAGTATTATATTTATTTtGTTTACACTCTAAGCTCATGTTTATATACGAGTGGATTGGACTCCACGATATTGTCGATAGGACCCACTCCATATATTTTTCACACTTTTGTAATATAAAGAATTTTCTTAAGATGGAACTAAATATATTATGTAATTAGGCAATATAGGTACGTTGGCATGAAATTAATATTTAGTAAGAAGTTGGAAATCGGAATGGCCACCATGTATAACATATTGCCAAGTTAATATACATGAAAGTAGTTGTACTTTTTAGACACCCTAATTATATTTATTGTTATTGTTTTTGTAAAAGGTCGAAAAAACAAATATAATAAAGTTGTCCTATTTTTTTTtACTTAATACGATAGTTTATTAACTATGGGAGGAAAAATAGTGGGTAGAATATTTATATCAAATTTTATCTCGGTGGACAGTTCTTATTCTATTTTGATTAATAAGCTTTTTACATTTTATTTTAATCTTTAAATATTATATCTAAAACCATTTAAATCATTATCTTTCATTTTCTTTTATATAAATTGTTTAACAATGACTTCACAACTCATCTTTTGTTTAAAACTTTAAACGGGTTAGGTAGTGGTAGTTAAACTATCAAATTTAATAATGAATGATAAATAATTGATGAAAAAATTAACACAATCTCCAAATTCTTATTATACAGATTACAAATTTATATACTAAATTTAGAAATATAATAGTTAAAAAGTTAAAAACTACCAATAATAACAATTTATAACCCTAAACTTTTGGAAATAATCAATTATAATTAAAACTCTTAATTATATTAATTTCATTAAATTTTTAGTAAATTGTGTTGGATTTGGAAAAAAATGGTGCGTTAAGACTTTTGTTTGCTTCAAATAAATATAATACACTAATTTTATAAGTATTTATTAAAATCTTTAGCTAATTTTTATTTTtGAAAATCGCTATAATTGTAATTGCACAAAATGGTATGATTTAGCCCTTGAACTGCTTACTATTTTTAATTTATAACGAACATTACAAATGTGGATTGTTTGGGCACAGGAAGCACTTGGAATCTTGGCAATTCCACCAGCCTTGGTAGTCTTGTTGGGGTTCTTCATACATGAGACACCATCCCCCACCCTTCAATATAATGGGAAGTCCAAGGTCCATCTCCCCCTAAcAATATCTTCCAACTTTACCAATTCTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNaaaGTaCtAaTTTTGCTTTTttATCtGTTTTtAAAaCATGTGCAAGAATTTCACAATCATCCATTTGAATCAAGATATTTATGaGAAAATGTAAGAAGTATTTTGAATAGTAAAATGAGAGACACAAAAATACAACTCTGACCAAAATGTATCTAAGTATTATTGGGACTATTACATGACATATGTAGGGAGGAAGTATAAAGCAAGTGGGAGTGGCCATAAGAGACACGTGCAAAGCCATCAAATATCCACACGTGTGGAGGCCTTCCCTTTTTATGTTTTTATGCCTTTCTCTTAGCATAAGTACCCACGAGGGACAATTTTATTGGTACACAGACAAGAAAGCTGGACCTGCCTTTTCTCAGGTAATTCTACATGTCGTCCTGGTCTCTTTTACTCAAATTTGCGTAAAAACGTCATGTCGTTTTCTTCACATTCTTGAATCGCAGGAATCGGTAGGCCTAATTTACGCAGTCGGCTCAGCGGCGTCACTAATTGGAGTACTAATCTACCACAAAACCCTTAGAGACTTGAAATTCCGACGAATCCTCTTCTTCGCTCAACTCTTTTACGGCGTCGCCGGATTTCTCGACGTCATCTTCGTCCTTCGTTGGAATTTAGCCTTAAGAATTCCAGATGAGTTATTCGTGGTGATTGAAGAGTGCGTTACTCGAATCGTGACGCGAATCCGATGGACGCCGATGATGGTTCTGAACACTCGACTATGTTCCGTTGGAATCGAAGGGACTTTCTTTGCATTATTGGCTTGTATTGATAGTTTGGGGACGTTGTGCTCGAAATGGAGCGGGGGATTGGTGCTCCATGCGTTTGGAGTAACGAGGAGTGATTTTAGGAACTTGTGGTTGGTGGTTCTGCTTCGAACTGTGTTGAGATTTGTGGTTGTGGGGTTCGTTTTtCTTGTTCCCGATGCGAATCAAACAGACATTTTGATTCCGGGTGATCATACGATGGTTAGAAAGAGTAGTTCGGAAGATGGGGATAACATTCCTCTTGTTTCTATGAAAAGCGAGGGTCGAGATTAG

mRNA sequence

ATGTTACAGCCATTCCGGTGGCTTAAAATGCTTTCAACGGAGCTACATCCAAGTTTCATCGCCGGAGTAATCCTCATCTACGGTCTAAGCCACGGCTTCTCCGGCTCTTATTTCAAAGTCGTTTCCGATTACTACTGGAAAGACGTACAAAAGCTACAACCCTCAGTAGTTCAGATCTACATCGGGATATACACGATTCCGTGGGTGATGAAACCAATTTGGGGGTTATTAACGGACGCGTTTCCAGTGAGAGGGTATTTGCGGCGGCCGTATTTCGTGATTTCGGGGGTGGTTGGAGCAACGGCGGCGGTTGGGATGGCAGTGAAAGGGGGATTGGGGGTTTTGGAAGCATTGGGGCTTTTGATTGGGATTTCAGGGGCGATGGCGATTGCGGATGTGACGATTGATGCTTGCATTGTGAGAAATAGCATTGAAGTTCAGTGGTTAGCCCAAGATTTGCAAAGCTTGTGTGGAGCTTGTTCTTCGATTGGGCATTTGATTGGATATTCAACTAGTGGATTTTTTGTTCATCTTCTTGGCGCTCAGGGAGGAAGTATAAAGCAAGTGGGAGTGGCCATAAGAGACACGTGCAAAGCCATCAAATATCCACACGTGTGGAGGCCTTCCCTTTTTATGTTTTTATGCCTTTCTCTTAGCATAAGTACCCACGAGGGACAATTTTATTGGTACACAGACAAGAAAGCTGGACCTGCCTTTTCTCAGGAATCGGTAGGCCTAATTTACGCAGTCGGCTCAGCGGCGTCACTAATTGGAGTACTAATCTACCACAAAACCCTTAGAGACTTGAAATTCCGACGAATCCTCTTCTTCGCTCAACTCTTTTACGGCGTCGCCGGATTTCTCGACGTCATCTTCGTCCTTCGTTGGAATTTAGCCTTAAGAATTCCAGATGAGTTATTCGTGGTGATTGAAGAGTGCGTTACTCGAATCGTGACGCGAATCCGATGGACGCCGATGATGGTTCTGAACACTCGACTATGTTCCGTTGGAATCGAAGGGACTTTCTTTGCATTATTGGCTTGTATTGATAGTTTGGGGACGTTGTGCTCGAAATGGAGCGGGGGATTGGTGCTCCATGCGTTTGGAGTAACGAGGAGTGATTTTAGGAACTTGTGGTTGGTGGTTCTGCTTCGAACTGTGTTGAGATTTGTGGTTGTGGGGTTCGTTTTTCTTGTTCCCGATGCGAATCAAACAGACATTTTGATTCCGGGTGATCATACGATGGTTAGAAAGAGTAGTTCGGAAGATGGGGATAACATTCCTCTTGTTTCTATGAAAAGCGAGGGTCGAGATTAG

Coding sequence (CDS)

ATGTTACAGCCATTCCGGTGGCTTAAAATGCTTTCAACGGAGCTACATCCAAGTTTCATCGCCGGAGTAATCCTCATCTACGGTCTAAGCCACGGCTTCTCCGGCTCTTATTTCAAAGTCGTTTCCGATTACTACTGGAAAGACGTACAAAAGCTACAACCCTCAGTAGTTCAGATCTACATCGGGATATACACGATTCCGTGGGTGATGAAACCAATTTGGGGGTTATTAACGGACGCGTTTCCAGTGAGAGGGTATTTGCGGCGGCCGTATTTCGTGATTTCGGGGGTGGTTGGaGCAACGGCGGCGGTTGGGATGGCAGTgAAAGGGGGATTGGGGGTTTTGGAAGCATTGGGGCTTTTGATTGGGATTTCAGGGGCGATGGCGATTGCGGATGTGACGATTGATGCTTGCATTGTGAGAAATAGCATTGAAGTTCAGTGGTTAGCCCAAGATTTGCAAAGCTTGTGTGGAGCTTGTTCTTCGATTGGGCATTTGATTGGATATTCAACTAGTGGATTTTTTGTTCATCTTCTTGGCGCTCAGGGAGGAAGTATAAAGCAAGTGGGAGTGGCCATAAGAGACACGTGCAAAGCCATCAAATATCCACACGTGTGGAGGCCTTCCCTTTTTATGTTTTTATGCCTTTCTCTTAGCATAAGTACCCACGAGGGACAATTTTATTGGTACACAGACAAGAAAGCTGGACCTGCCTTTTCTCAGGAATCGGTAGGCCTAATTTACGCAGTCGGCTCAGCGGCGTCACTAATTGGAGTACTAATCTACCACAAAACCCTTAGAGACTTGAAATTCCGACGAATCCTCTTCTTCGCTCAACTCTTTTACGGCGTCGCCGGATTTCTCGACGTCATCTTCGTCCTTCGTTGGAATTTAGCCTTAAGAATTCCAGATGAGTTATTCGTGGTGATTGAAGAGTGCGTTACTCGAATCGTGACGCGAATCCGATGGACGCCGATGATGGTTCTGAACACTCGACTATGTTCCGTTGGAATCGAAGGGACTTTCTTTGCATTATTGGCTTGTATTGATAGTTTGGGGACGTTGTGCTCGAAATGGAGCGGGGGATTGGTGCTCCATGCGTTTGGAGTAACGAGGAGTGATTTTAGGAACTTGTGGTTGGTGGTTCTGCTTCGAACTGTGTTGAGATTTGTGGTTGTGGGGTTCGTTTTtCTTGTTCCCGATGCGAATCAAACAGACATTTTGATTCCGGGTGATCATACGATGGTTAGAAAGAGTAGTTCGGAAGATGGGGATAACATTCCTCTTGTTTCTATGAAAAGCGAGGGTCGAGATTAG

Protein sequence

MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGLLIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLGAQGGSIKQVGVAIRDTCKAIKYPHVWRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRTVLRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLVSMKSEGRD*
BLAST of Cucsa.162860.2 vs. Swiss-Prot
Match: FBT6_ARATH (Probable folate-biopterin transporter 6 OS=Arabidopsis thaliana GN=At5g10820 PE=2 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 8.3e-158
Identity = 266/463 (57.45%), Postives = 336/463 (72.57%), Query Frame = 1

Query: 1   MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY 60
           ++QPF+WL+MLS+ L+ SF+ GV+L+YG++ GFSGS FKVV+DYYWKDVQ++QPSVVQ+Y
Sbjct: 39  LIQPFQWLQMLSSRLNLSFVLGVVLVYGVNQGFSGSIFKVVTDYYWKDVQQVQPSVVQLY 98

Query: 61  IGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGL 120
           +G+Y IPWVM+PIWGL TD FP++GY R+PYFV+SGV+G  +A+ + V G L    AL  
Sbjct: 99  MGLYYIPWVMRPIWGLFTDVFPIKGYKRKPYFVVSGVLGLVSAIAIVVLGKLPAALALSC 158

Query: 121 LIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLG 180
           L+G+S AMAIADV IDACI  NSI ++ LA D+QSLC  CSS G L+GY+TSG FVH LG
Sbjct: 159 LLGVSAAMAIADVVIDACIATNSINIRSLAPDIQSLCMVCSSAGALVGYATSGVFVHQLG 218

Query: 181 AQGG---------------------------------SIKQVGVAIRDTCKAIKYPHVWR 240
            QG                                      +GVA++  CK +KYP VW+
Sbjct: 219 PQGALGVLAFSPATIVILGFFIYEKRSSTVPTQKTKKDTDGLGVAVKGMCKTVKYPEVWK 278

Query: 241 PSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLR 300
           PSL+MF+ L+L+ISTHEG FYWYTD  AGPAFSQE VG+IYAVG+ AS+ GVLIYHK L+
Sbjct: 279 PSLYMFISLALNISTHEGHFYWYTDPTAGPAFSQEFVGIIYAVGALASMFGVLIYHKKLK 338

Query: 301 DLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTPMM 360
              FR ILFFAQL Y  +G LD++F+ RWNL L IPD LFV+ EE  T+++++IRW PM+
Sbjct: 339 GYSFRNILFFAQLLYVFSGMLDLVFIKRWNLTLGIPDSLFVITEESFTKMISKIRWIPMV 398

Query: 361 VLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRTV 420
           VL+TRLC +GIEGTFFA L CIDS G L SKW GG VLHAFGVTR DF NLWLV+L+R +
Sbjct: 399 VLSTRLCPLGIEGTFFAFLMCIDSFGQLASKWGGGFVLHAFGVTRHDFGNLWLVILIRNI 458

Query: 421 LRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLV 431
           LR V V FVFLVPD++  D L+P D    ++S  +D D+I L+
Sbjct: 459 LRLVTVCFVFLVPDSDHLDDLVPSDVLPKKQSEDDDDDDIKLL 501

BLAST of Cucsa.162860.2 vs. Swiss-Prot
Match: FBT3_ARATH (Probable folate-biopterin transporter 3 OS=Arabidopsis thaliana GN=At1g79710 PE=2 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 3.5e-116
Identity = 204/463 (44.06%), Postives = 300/463 (64.79%), Query Frame = 1

Query: 3   QPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYIG 62
           +P RWL+ML  ELH SF  GVI++YG+S G      KV + YY+KD QK+QPS  QIY+G
Sbjct: 33  RPLRWLRMLIEELHWSFFFGVIIVYGVSQGLGKGLSKVSTQYYFKDEQKIQPSQAQIYVG 92

Query: 63  IYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGLLI 122
           +  IPW++KP+WGLLTD  PV GY RRPYF+++G +   + + + +   L +  AL  L+
Sbjct: 93  LIQIPWIIKPLWGLLTDVVPVLGYRRRPYFILAGFLAMISMMVLWLHTNLHLALALSCLV 152

Query: 123 GISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLGAQ 182
             S  +AIADVTIDAC+ + SI    LA D+QSLCG CSSIG L+G+S SG  VHL+G++
Sbjct: 153 AGSAGVAIADVTIDACVTQCSISHPTLAADMQSLCGLCSSIGSLVGFSLSGVLVHLVGSK 212

Query: 183 G---------GSIKQVGVAIRDT---------------------CKAIKYPHVWRPSLFM 242
           G         G +  VG+ ++++                      K  +Y  VWRP LFM
Sbjct: 213 GVYGLLGVTAGLLVVVGMVLKESPSRSLGRKHVNDKFLDAGSAIWKTFQYGEVWRPCLFM 272

Query: 243 FLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDLKFR 302
            L  ++S+  HEG FYWYTD K GP+FS+E+VG I + G+  SL+G+L+Y   L++  FR
Sbjct: 273 LLSAAVSLHIHEGMFYWYTDSKDGPSFSKEAVGSIMSFGAIGSLVGILLYQNFLKNFPFR 332

Query: 303 RILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTPMMVLNTR 362
            ++F+A     ++GFLD+I VLR NL L +PD  F+V++E V+ +++RI+W P++VL+++
Sbjct: 333 NVVFWALSLSVLSGFLDLILVLRINLKLGLPDYFFIVVDEFVSHMISRIKWLPLLVLSSK 392

Query: 363 LCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRTVLRFVV 422
           LC  G+EGTFFALL  I+ +G L S W GG++LHA  VTR+ F NLWLV+++R++LR + 
Sbjct: 393 LCPAGMEGTFFALLMSIEHIGHLVSSWGGGVLLHALKVTRTQFDNLWLVIVIRSLLRVIP 452

Query: 423 VGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLVSMKSE 436
           +G VFL+P+ +    ++P +    R S   D + I + ++ S+
Sbjct: 453 IGLVFLIPNVDSNSTILPAEMLTSRISEGVDTEKIEMTALISD 495

BLAST of Cucsa.162860.2 vs. Swiss-Prot
Match: FBT2_ARATH (Probable folate-biopterin transporter 2 OS=Arabidopsis thaliana GN=At5g25050 PE=2 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 4.9e-110
Identity = 203/463 (43.84%), Postives = 290/463 (62.63%), Query Frame = 1

Query: 4   PFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYIGI 63
           P RWLKMLS+ELH SF+ GV+ +YG++ G  GS  +V ++YY KDVQK+QPS  Q    I
Sbjct: 30  PVRWLKMLSSELHWSFVFGVVSLYGINQGLGGSLGRVATEYYMKDVQKVQPSESQALTAI 89

Query: 64  YTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGLLIG 123
             IPW++KP+WG+LTD  P+ G+ RRPYF+++GV+G  + + +++   L +  AL  +  
Sbjct: 90  TKIPWIIKPLWGILTDVLPIFGFHRRPYFILAGVLGVVSLLFISLHSNLHLYLALFWMTI 149

Query: 124 ISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLGAQ- 183
            S AMAIADVTIDAC   NSI+   LA D+QSLC   SSIG L+G+  SG  VHL+G++ 
Sbjct: 150 SSAAMAIADVTIDACTAYNSIKHPSLASDMQSLCSLSSSIGALLGFFMSGILVHLVGSKG 209

Query: 184 -------------------------GGSIKQVGVAIRDTCKA----IKYPHVWRPSLFMF 243
                                    G S KQV     D  KA    +K   VWRPSL+M+
Sbjct: 210 VFGLLTFPFALVSVVGIVFSEPHVPGFSYKQVNQKFTDAGKAMWRTMKCSDVWRPSLYMY 269

Query: 244 LCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDLKFRR 303
           + L+L ++ HEG FYW+TD K GP F+QE+VG I ++GS  S++   +Y   L+D  FR 
Sbjct: 270 ISLTLGLNIHEGLFYWFTDSKDGPLFAQETVGFILSIGSIGSILAATLYQLVLKDHPFRG 329

Query: 304 ILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTPMMVLNTRL 363
           +  + QL + ++G LD+I V R NL   +PD LF+V++E V++++ R++W P++VL ++L
Sbjct: 330 LCLWTQLLFALSGMLDLILVFRLNLKFGLPDYLFIVVDEIVSQMIGRLKWMPLLVLTSKL 389

Query: 364 CSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRTVLRFVVV 423
           C  GIEGTFFALL  ID+ G + S W GG++LH   VTR++F NLWL VL+R V+R + +
Sbjct: 390 CPHGIEGTFFALLMSIDNAGLMTSSWLGGIMLHVLKVTRTEFGNLWLAVLVRNVMRLLPL 449

Query: 424 GFVFLVPDANQTDILIP----GDHTMVRKSSSEDGDNIPLVSM 433
            F+FLVP  +Q    +P    G+ +   K   E   N+ L S+
Sbjct: 450 CFLFLVPKGDQNTFKLPDEIMGEDSDEEKDEKEGTRNLELASL 492

BLAST of Cucsa.162860.2 vs. Swiss-Prot
Match: FBT5_ARATH (Probable folate-biopterin transporter 5 OS=Arabidopsis thaliana GN=At5g25040 PE=2 SV=2)

HSP 1 Score: 370.5 bits (950), Expect = 2.5e-101
Identity = 194/457 (42.45%), Postives = 282/457 (61.71%), Query Frame = 1

Query: 4   PFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYIGI 63
           P RWLKML+++LH S++  V+   G++ GF  S   V +DYY KDVQK+QPS  Q    I
Sbjct: 30  PVRWLKMLASKLHWSYVFAVVSNCGINQGFGYSLGHVATDYYMKDVQKVQPSQYQALSAI 89

Query: 64  YTIPWVM-KPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGLLI 123
             I W++ KP++G+LTD  P+ G+ RRPYF+++GV+G  + + ++++  L +  AL  + 
Sbjct: 90  TRISWIIFKPLFGILTDVLPIFGFHRRPYFILAGVIGVVSLLFISLQSNLHLYLALFWMT 149

Query: 124 GISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLGAQ 183
             S AMAIADVTIDAC   NS +   LA D+QSLC    SIG L+G+  SG  VHL+G++
Sbjct: 150 ISSAAMAIADVTIDACTTYNSNKHPSLASDMQSLCSLSYSIGELLGFFMSGILVHLVGSK 209

Query: 184 G---------GSIKQVGV------------------AIRDTCKAIKYPHVWRPSLFMFLC 243
           G           +  VGV                  A++   + IK   VW+PSL+MF+ 
Sbjct: 210 GVFGLLTFTFALVSVVGVLFSEPRVHGFSFKQNFTNAMKAMWRTIKCSDVWQPSLYMFIT 269

Query: 244 LSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDLKFRRIL 303
            +L +   EG FYW+TD K GP F+QE+VG I ++GS  S++GVL+Y+  L+D  FR++ 
Sbjct: 270 RALGLDIKEGLFYWFTDSKDGPLFAQETVGFILSIGSIGSILGVLLYNLRLKDHPFRKLF 329

Query: 304 FFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTPMMVLNTRLCS 363
            + QL + ++G  D+I VLR NL   +PD LF+V++  V++++ R+ W  + VLNT+LC 
Sbjct: 330 LWTQLLFALSGMFDLILVLRLNLKFGLPDYLFIVVDGIVSKMIIRLTWMVIFVLNTKLCP 389

Query: 364 VGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRTVLRFVVVGF 423
            GIEGTFFALL  ID+ G + S W GG +LH   VTR++F NLWL VL+R V+R + + F
Sbjct: 390 HGIEGTFFALLMSIDNAGLMTSSWLGGKMLHVLKVTRTEFGNLWLAVLVRNVMRLLPICF 449

Query: 424 VFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLVSM 433
           +FLVP  +Q    +P +  M   S  E   N+ L S+
Sbjct: 450 LFLVPQGDQNTFKLPAE-IMGEDSEEEGTRNLELASL 485

BLAST of Cucsa.162860.2 vs. Swiss-Prot
Match: FBT_SYNY3 (Folate-biopterin transporter OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=slr0642 PE=1 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.2e-36
Identity = 125/439 (28.47%), Postives = 209/439 (47.61%), Query Frame = 1

Query: 19  FIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIPWVMKPIWGLLT 78
           F+ GV+   GLS        ++   ++ KD   L P+ +   IG+   PW++KP+ GL++
Sbjct: 44  FVQGVL---GLS--------RLAVSFFLKDELGLSPAAMGALIGLGAAPWILKPVLGLMS 103

Query: 79  DAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGLLIGISGAMAIADVTIDAC 138
           D  P+ GY RR Y  +SG++G+   +  A     G    L LL   S ++AI DV +D+ 
Sbjct: 104 DTVPLFGYRRRSYLWLSGLMGSAGWLLFAAWVSSGTQAGLVLLF-TSLSVAIGDVIVDSL 163

Query: 139 IVRNSIEVQWLAQ--DLQSLCGACSSIGHLIGYSTSG----------------FFVHLLG 198
           +V  + + + LAQ   LQSL    +++G +I    SG                 F  L  
Sbjct: 164 VVERA-QRESLAQVGSLQSLTWGAAAVGGIITAYASGALLEWFSTRTVFAITAIFPLLTV 223

Query: 199 AQGGSIKQVGVA-----------IRDTCKAIKYPHVWRPSLFMFLCLSLSISTHEGQFYW 258
                I +V  A           I+   +A++   +  P+LF+F     +  + E  F++
Sbjct: 224 GAAFLISEVSTAEEEEKPQPKAQIKLVWQAVRQKTILLPTLFIFFW--QATPSAESAFFY 283

Query: 259 YTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDLKFRRILFFAQLFYGVAGFLD 318
           +T  + G  F  + +G +  V S A LIGV +Y + L+ L FR I+ ++ +   + G   
Sbjct: 284 FTTNELG--FEPKFLGRVRLVTSVAGLIGVGLYQRFLKTLPFRVIMGWSTVISSLLGLTT 343

Query: 319 VIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTPMMVLNTRLCSVGIEGTFFALLACI 378
           +I +   N A+ I D  F + +  +  +  +I + P++VL  RLC  GIE T FALL  +
Sbjct: 344 LILITHANRAMGIDDHWFSLGDSIILTVTGQIAFMPVLVLAARLCPPGIEATLFALLMSV 403

Query: 379 DSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRTVLRFVVVGFVFLVPDANQTDILI 429
            +L  + S   G L+ H  GVT + F NL L+V++  +   + + F+ L+P         
Sbjct: 404 MNLAGVLSFEVGSLLTHWLGVTETQFDNLALLVIITNLSTLLPLPFLGLLP--------- 456

BLAST of Cucsa.162860.2 vs. TrEMBL
Match: A0A0A0LMJ9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G382710 PE=4 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 6.7e-247
Identity = 436/473 (92.18%), Postives = 438/473 (92.60%), Query Frame = 1

Query: 1   MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY 60
           MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY
Sbjct: 73  MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY 132

Query: 61  IGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGL 120
           IGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGL
Sbjct: 133 IGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGL 192

Query: 121 LIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLG 180
           LIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLG
Sbjct: 193 LIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLG 252

Query: 181 -----------------------------------AQGGSIKQVGVAIRDTCKAIKYPHV 240
                                              ++GGSIKQVGVAIRDTCKAIKYPHV
Sbjct: 253 AQEALGILAIPPALVVLLGFFIHETPSPTLQYNGKSKGGSIKQVGVAIRDTCKAIKYPHV 312

Query: 241 WRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT 300
           WRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT
Sbjct: 313 WRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT 372

Query: 301 LRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTP 360
           LRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTP
Sbjct: 373 LRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTP 432

Query: 361 MMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLR 420
           MMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLR
Sbjct: 433 MMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLR 492

Query: 421 TVLRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLVSMKSEGRD 439
           TVLRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLVSMKSEGRD
Sbjct: 493 TVLRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLVSMKSEGRD 545

BLAST of Cucsa.162860.2 vs. TrEMBL
Match: W9RP11_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_025493 PE=4 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 7.8e-171
Identity = 296/468 (63.25%), Postives = 366/468 (78.21%), Query Frame = 1

Query: 2   LQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYI 61
           L+PFRWL+MLS++L P+F+AGV+L+YGL+ GFSGS+FKVVSDYYWKDVQK+QPS VQ+Y 
Sbjct: 39  LEPFRWLQMLSSQLSPTFVAGVVLVYGLNQGFSGSFFKVVSDYYWKDVQKVQPSAVQLYS 98

Query: 62  GIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGLL 121
           G+YTIPWVMKPIWGLLTD FPVRG+ RRPYFV++GVVGA +AV +A+ GG+    A+G L
Sbjct: 99  GLYTIPWVMKPIWGLLTDVFPVRGFRRRPYFVLAGVVGALSAVAVALDGGMSAAAAVGCL 158

Query: 122 IGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLGA 181
           +G+S A+AIADVTIDACI RNSIE++ LA DLQSLCG CSS+G L+GYS+SGFFVH LG 
Sbjct: 159 MGLSAAVAIADVTIDACIARNSIEIRALASDLQSLCGFCSSVGALLGYSSSGFFVHHLGP 218

Query: 182 QGG-------------------------------SIKQVGVAIRDTCKAIKYPHVWRPSL 241
           QG                                +++++GVA++   K IK+P VW+PSL
Sbjct: 219 QGALGLLAVPPALLVVLGFFIYEQRTISSHSEKKAVEKLGVAMKGMYKTIKFPQVWKPSL 278

Query: 242 FMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDLK 301
           +M+L L+LSISTHEGQFYWYTD KAGPAFSQE VG+IYA+G+ AS++GVLIYHKTL+D  
Sbjct: 279 YMYLSLALSISTHEGQFYWYTDPKAGPAFSQEFVGIIYAIGAMASIVGVLIYHKTLKDHP 338

Query: 302 FRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTPMMVLN 361
           FR ILF AQL YGV+G +D+IF+LRWNLAL IPD  FVV+EECV RIV+RIRW PM+VL+
Sbjct: 339 FRNILFLAQLLYGVSGMIDLIFILRWNLALGIPDYFFVVMEECVHRIVSRIRWMPMIVLS 398

Query: 362 TRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRTVLRF 421
           +RLC +GIEGTFFALL CIDSLG L SKW GG+VLH   VTR+DF NLWLVVL+R VLRF
Sbjct: 399 SRLCPLGIEGTFFALLMCIDSLGGLSSKWGGGMVLHLLHVTRTDFTNLWLVVLIRNVLRF 458

Query: 422 VVVGFVFLVPDANQTDILIPGDHTMVRKSSS---EDGDNIPLVSMKSE 436
             + F+FLVP A Q+D+LIP D  + + S+S   ED +N+ LV +  E
Sbjct: 459 STLAFIFLVPKAGQSDVLIPSD-LLTKNSTSIDEEDHENLELVPLNVE 505

BLAST of Cucsa.162860.2 vs. TrEMBL
Match: A0A061GIF0_THECC (Major facilitator superfamily protein OS=Theobroma cacao GN=TCM_036893 PE=4 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 1.5e-166
Identity = 285/462 (61.69%), Postives = 355/462 (76.84%), Query Frame = 1

Query: 2   LQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYI 61
           L+PF+WL+MLS++L+P+FI G++L+YGLS GFS S+FKVV+DYYWKDVQK+QPSVVQ+YI
Sbjct: 35  LEPFQWLQMLSSQLNPTFIFGIVLVYGLSQGFSSSFFKVVTDYYWKDVQKVQPSVVQLYI 94

Query: 62  GIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGLL 121
           G+Y IPW+MKP+WGL TD FP++GY RRPYFV++GVVG  +A+ +A+ G L    ALG L
Sbjct: 95  GLYYIPWIMKPVWGLFTDVFPIKGYRRRPYFVLAGVVGGASALMVALFGNLPAALALGCL 154

Query: 122 IGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLGA 181
           IG++  MAIADVTIDACI RNSI+V+ LA D+QSLCG CSS G LIGYSTSGFFVH LGA
Sbjct: 155 IGVAAGMAIADVTIDACIARNSIKVRSLAPDMQSLCGFCSSAGALIGYSTSGFFVHHLGA 214

Query: 182 QGGSI--------------------------------KQVGVAIRDTCKAIKYPHVWRPS 241
           QG  I                                + +GVA+R   + IK+P VW+PS
Sbjct: 215 QGALILLAIPPVFITVLGFVIYEMRSTSLQSEKQKAMETLGVALRGMYQTIKFPQVWKPS 274

Query: 242 LFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDL 301
           L+M+L L+LSISTHEGQFYWYTD KAGPAFSQE VG+IYA+G+ AS++GVLIYHKTL+D+
Sbjct: 275 LYMYLSLALSISTHEGQFYWYTDPKAGPAFSQEFVGVIYAIGAMASMVGVLIYHKTLKDI 334

Query: 302 KFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTPMMVL 361
            FR +LFFAQL YG +G LD+IF+LRWNL L IPD  FV++EECV+RI++RIRW PM+VL
Sbjct: 335 PFRNLLFFAQLLYGTSGMLDLIFILRWNLVLGIPDYFFVIMEECVSRIISRIRWIPMIVL 394

Query: 362 NTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRTVLR 421
           +TRLC +GIEGTFFALL CIDSLG+L SKW GG+VLH   VTR+DF NLWLVVL+R  LR
Sbjct: 395 STRLCPLGIEGTFFALLMCIDSLGSLTSKWGGGIVLHTLHVTRTDFTNLWLVVLIRNTLR 454

Query: 422 FVVVGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLVS 432
           F  +G +FLVP A+Q+D LIP D      + + D + + LVS
Sbjct: 455 FATLGLIFLVPKADQSDALIPPDILTNNSTVTADDEGLELVS 496

BLAST of Cucsa.162860.2 vs. TrEMBL
Match: A0A0D2UH96_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_009G063700 PE=4 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 3.2e-164
Identity = 280/461 (60.74%), Postives = 352/461 (76.36%), Query Frame = 1

Query: 2   LQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYI 61
           L+P +W++MLS++L+P+FI G++++YGL+ GFSGS+FKVV+DYYWKDVQK+QPSVVQ+YI
Sbjct: 46  LEPIQWIQMLSSQLNPTFIIGIVIVYGLNLGFSGSFFKVVTDYYWKDVQKVQPSVVQLYI 105

Query: 62  GIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGLL 121
           G+Y IPW+MKP+WGLLTD FP+RGY RRPYFV++GV+G  +A+ +A+ G L    AL   
Sbjct: 106 GLYYIPWIMKPVWGLLTDVFPIRGYRRRPYFVLAGVLGGVSALMVALIGNLPAALALSCF 165

Query: 122 IGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLGA 181
           IGI+  MAIADVTIDACI  NSIEV+ LA D+QSLCG CSS G LIGYSTSGFFVH LGA
Sbjct: 166 IGIAAGMAIADVTIDACIATNSIEVRSLAPDMQSLCGFCSSAGALIGYSTSGFFVHHLGA 225

Query: 182 QGGSI---------------------------KQ-----VGVAIRDTCKAIKYPHVWRPS 241
           QG  I                           KQ     +GVA++   + IK+P VW+PS
Sbjct: 226 QGALILMSIPPVFITVLGFVIYEMRSPCLHPEKQKKMGTLGVAVKGMYQTIKFPQVWKPS 285

Query: 242 LFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDL 301
           L+M+L L+LSISTHEGQFYWYTD KAGP FSQESVG IYA+G+ AS++GVLIYHKTL+D+
Sbjct: 286 LYMYLSLALSISTHEGQFYWYTDAKAGPGFSQESVGAIYAIGAMASMVGVLIYHKTLKDI 345

Query: 302 KFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTPMMVL 361
            FR +LFFAQL YG +G LD+IF+LRWNL L IPD  FV+ EEC++RI++RIRW PM+VL
Sbjct: 346 PFRNLLFFAQLLYGASGMLDLIFILRWNLVLGIPDYFFVITEECISRIISRIRWIPMIVL 405

Query: 362 NTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRTVLR 421
           +TRLC +GIEGTFFALL CIDSLG+L SKW GG+VLH F VTR+DF NLWLV+L+R +LR
Sbjct: 406 STRLCPLGIEGTFFALLMCIDSLGSLTSKWGGGMVLHVFHVTRTDFTNLWLVILIRNILR 465

Query: 422 FVVVGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLV 431
              +G +FLVP A+ +D LIP D  M   + + D + + LV
Sbjct: 466 IATLGLIFLVPKADPSDALIPQDILMTHSTVTSDDEGLELV 506

BLAST of Cucsa.162860.2 vs. TrEMBL
Match: D7SV54_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0008g06790 PE=4 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 3.3e-161
Identity = 275/458 (60.04%), Postives = 346/458 (75.55%), Query Frame = 1

Query: 1   MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY 60
           ++QPF+WL+ML  +L+P+FI G+ L+YGLS GFS S+FKVV+D+YWKDVQK+QPSVVQ+Y
Sbjct: 49  LIQPFQWLQMLCKQLNPTFILGIALVYGLSQGFSNSFFKVVTDFYWKDVQKVQPSVVQLY 108

Query: 61  IGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGL 120
           IG Y IPW+MKP+WGLLTD FPV GY RRPYFV +GV+G  +A  +A+ G L V  ALG 
Sbjct: 109 IGFYYIPWIMKPVWGLLTDVFPVMGYRRRPYFVAAGVIGTVSAAVVAISGNLPVAVALGC 168

Query: 121 LIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLG 180
           LIGI+  +AIADVTIDACI RNSIE++ LA D+QS  G CSS G L+GYS+SGFFVH LG
Sbjct: 169 LIGITAGVAIADVTIDACIARNSIEIRSLAADMQSFSGFCSSAGALLGYSSSGFFVHHLG 228

Query: 181 AQGG---------------------------------SIKQVGVAIRDTCKAIKYPHVWR 240
           AQG                                  ++++V +AIRD  + I+ P VW+
Sbjct: 229 AQGALGILVGPPAMLVVLGFVIYESRAPNLASNNKRKAMEKVDIAIRDMYRTIRCPQVWK 288

Query: 241 PSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLR 300
           PSL+M+L L+LSISTHEGQFYWYTD KAGPAFSQE VG+IYAVG+ AS++G+LIYHKTL+
Sbjct: 289 PSLYMYLSLALSISTHEGQFYWYTDPKAGPAFSQEFVGMIYAVGALASILGILIYHKTLK 348

Query: 301 DLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTPMM 360
           D  FR +LFFAQL Y ++G LDVIF+LRWNLAL +PD LFVV+EEC++R+V R+RW PM+
Sbjct: 349 DCPFRTLLFFAQLIYAISGILDVIFILRWNLALGVPDYLFVVMEECISRVVARVRWMPMI 408

Query: 361 VLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRTV 420
           +L+T+LC +GIEGTFFALL CIDSLG+L SKW+GG+VLH   VTRSDF NLWLVVL+R +
Sbjct: 409 ILSTKLCPLGIEGTFFALLMCIDSLGSLSSKWAGGMVLHLLHVTRSDFTNLWLVVLIRNI 468

Query: 421 LRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSSEDGD 426
           LR   +G +FLVP+  Q+D +I  D      S SE+ D
Sbjct: 469 LRLATLGLIFLVPNVGQSDEVISSDFLNDTSSVSEEDD 506

BLAST of Cucsa.162860.2 vs. TAIR10
Match: AT5G10820.1 (AT5G10820.1 Major facilitator superfamily protein)

HSP 1 Score: 558.1 bits (1437), Expect = 4.7e-159
Identity = 266/463 (57.45%), Postives = 336/463 (72.57%), Query Frame = 1

Query: 1   MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY 60
           ++QPF+WL+MLS+ L+ SF+ GV+L+YG++ GFSGS FKVV+DYYWKDVQ++QPSVVQ+Y
Sbjct: 39  LIQPFQWLQMLSSRLNLSFVLGVVLVYGVNQGFSGSIFKVVTDYYWKDVQQVQPSVVQLY 98

Query: 61  IGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGL 120
           +G+Y IPWVM+PIWGL TD FP++GY R+PYFV+SGV+G  +A+ + V G L    AL  
Sbjct: 99  MGLYYIPWVMRPIWGLFTDVFPIKGYKRKPYFVVSGVLGLVSAIAIVVLGKLPAALALSC 158

Query: 121 LIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLG 180
           L+G+S AMAIADV IDACI  NSI ++ LA D+QSLC  CSS G L+GY+TSG FVH LG
Sbjct: 159 LLGVSAAMAIADVVIDACIATNSINIRSLAPDIQSLCMVCSSAGALVGYATSGVFVHQLG 218

Query: 181 AQGG---------------------------------SIKQVGVAIRDTCKAIKYPHVWR 240
            QG                                      +GVA++  CK +KYP VW+
Sbjct: 219 PQGALGVLAFSPATIVILGFFIYEKRSSTVPTQKTKKDTDGLGVAVKGMCKTVKYPEVWK 278

Query: 241 PSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLR 300
           PSL+MF+ L+L+ISTHEG FYWYTD  AGPAFSQE VG+IYAVG+ AS+ GVLIYHK L+
Sbjct: 279 PSLYMFISLALNISTHEGHFYWYTDPTAGPAFSQEFVGIIYAVGALASMFGVLIYHKKLK 338

Query: 301 DLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTPMM 360
              FR ILFFAQL Y  +G LD++F+ RWNL L IPD LFV+ EE  T+++++IRW PM+
Sbjct: 339 GYSFRNILFFAQLLYVFSGMLDLVFIKRWNLTLGIPDSLFVITEESFTKMISKIRWIPMV 398

Query: 361 VLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRTV 420
           VL+TRLC +GIEGTFFA L CIDS G L SKW GG VLHAFGVTR DF NLWLV+L+R +
Sbjct: 399 VLSTRLCPLGIEGTFFAFLMCIDSFGQLASKWGGGFVLHAFGVTRHDFGNLWLVILIRNI 458

Query: 421 LRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLV 431
           LR V V FVFLVPD++  D L+P D    ++S  +D D+I L+
Sbjct: 459 LRLVTVCFVFLVPDSDHLDDLVPSDVLPKKQSEDDDDDDIKLL 501

BLAST of Cucsa.162860.2 vs. TAIR10
Match: AT1G79710.1 (AT1G79710.1 Major facilitator superfamily protein)

HSP 1 Score: 419.9 bits (1078), Expect = 2.0e-117
Identity = 204/463 (44.06%), Postives = 300/463 (64.79%), Query Frame = 1

Query: 3   QPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYIG 62
           +P RWL+ML  ELH SF  GVI++YG+S G      KV + YY+KD QK+QPS  QIY+G
Sbjct: 33  RPLRWLRMLIEELHWSFFFGVIIVYGVSQGLGKGLSKVSTQYYFKDEQKIQPSQAQIYVG 92

Query: 63  IYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGLLI 122
           +  IPW++KP+WGLLTD  PV GY RRPYF+++G +   + + + +   L +  AL  L+
Sbjct: 93  LIQIPWIIKPLWGLLTDVVPVLGYRRRPYFILAGFLAMISMMVLWLHTNLHLALALSCLV 152

Query: 123 GISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLGAQ 182
             S  +AIADVTIDAC+ + SI    LA D+QSLCG CSSIG L+G+S SG  VHL+G++
Sbjct: 153 AGSAGVAIADVTIDACVTQCSISHPTLAADMQSLCGLCSSIGSLVGFSLSGVLVHLVGSK 212

Query: 183 G---------GSIKQVGVAIRDT---------------------CKAIKYPHVWRPSLFM 242
           G         G +  VG+ ++++                      K  +Y  VWRP LFM
Sbjct: 213 GVYGLLGVTAGLLVVVGMVLKESPSRSLGRKHVNDKFLDAGSAIWKTFQYGEVWRPCLFM 272

Query: 243 FLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDLKFR 302
            L  ++S+  HEG FYWYTD K GP+FS+E+VG I + G+  SL+G+L+Y   L++  FR
Sbjct: 273 LLSAAVSLHIHEGMFYWYTDSKDGPSFSKEAVGSIMSFGAIGSLVGILLYQNFLKNFPFR 332

Query: 303 RILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTPMMVLNTR 362
            ++F+A     ++GFLD+I VLR NL L +PD  F+V++E V+ +++RI+W P++VL+++
Sbjct: 333 NVVFWALSLSVLSGFLDLILVLRINLKLGLPDYFFIVVDEFVSHMISRIKWLPLLVLSSK 392

Query: 363 LCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRTVLRFVV 422
           LC  G+EGTFFALL  I+ +G L S W GG++LHA  VTR+ F NLWLV+++R++LR + 
Sbjct: 393 LCPAGMEGTFFALLMSIEHIGHLVSSWGGGVLLHALKVTRTQFDNLWLVIVIRSLLRVIP 452

Query: 423 VGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLVSMKSE 436
           +G VFL+P+ +    ++P +    R S   D + I + ++ S+
Sbjct: 453 IGLVFLIPNVDSNSTILPAEMLTSRISEGVDTEKIEMTALISD 495

BLAST of Cucsa.162860.2 vs. TAIR10
Match: AT5G25050.1 (AT5G25050.1 Major facilitator superfamily protein)

HSP 1 Score: 399.4 bits (1025), Expect = 2.8e-111
Identity = 203/463 (43.84%), Postives = 290/463 (62.63%), Query Frame = 1

Query: 4   PFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYIGI 63
           P RWLKMLS+ELH SF+ GV+ +YG++ G  GS  +V ++YY KDVQK+QPS  Q    I
Sbjct: 30  PVRWLKMLSSELHWSFVFGVVSLYGINQGLGGSLGRVATEYYMKDVQKVQPSESQALTAI 89

Query: 64  YTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGLLIG 123
             IPW++KP+WG+LTD  P+ G+ RRPYF+++GV+G  + + +++   L +  AL  +  
Sbjct: 90  TKIPWIIKPLWGILTDVLPIFGFHRRPYFILAGVLGVVSLLFISLHSNLHLYLALFWMTI 149

Query: 124 ISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLGAQ- 183
            S AMAIADVTIDAC   NSI+   LA D+QSLC   SSIG L+G+  SG  VHL+G++ 
Sbjct: 150 SSAAMAIADVTIDACTAYNSIKHPSLASDMQSLCSLSSSIGALLGFFMSGILVHLVGSKG 209

Query: 184 -------------------------GGSIKQVGVAIRDTCKA----IKYPHVWRPSLFMF 243
                                    G S KQV     D  KA    +K   VWRPSL+M+
Sbjct: 210 VFGLLTFPFALVSVVGIVFSEPHVPGFSYKQVNQKFTDAGKAMWRTMKCSDVWRPSLYMY 269

Query: 244 LCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDLKFRR 303
           + L+L ++ HEG FYW+TD K GP F+QE+VG I ++GS  S++   +Y   L+D  FR 
Sbjct: 270 ISLTLGLNIHEGLFYWFTDSKDGPLFAQETVGFILSIGSIGSILAATLYQLVLKDHPFRG 329

Query: 304 ILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTPMMVLNTRL 363
           +  + QL + ++G LD+I V R NL   +PD LF+V++E V++++ R++W P++VL ++L
Sbjct: 330 LCLWTQLLFALSGMLDLILVFRLNLKFGLPDYLFIVVDEIVSQMIGRLKWMPLLVLTSKL 389

Query: 364 CSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRTVLRFVVV 423
           C  GIEGTFFALL  ID+ G + S W GG++LH   VTR++F NLWL VL+R V+R + +
Sbjct: 390 CPHGIEGTFFALLMSIDNAGLMTSSWLGGIMLHVLKVTRTEFGNLWLAVLVRNVMRLLPL 449

Query: 424 GFVFLVPDANQTDILIP----GDHTMVRKSSSEDGDNIPLVSM 433
            F+FLVP  +Q    +P    G+ +   K   E   N+ L S+
Sbjct: 450 CFLFLVPKGDQNTFKLPDEIMGEDSDEEKDEKEGTRNLELASL 492

BLAST of Cucsa.162860.2 vs. TAIR10
Match: AT5G25040.2 (AT5G25040.2 Major facilitator superfamily protein)

HSP 1 Score: 356.7 bits (914), Expect = 2.1e-98
Identity = 194/482 (40.25%), Postives = 282/482 (58.51%), Query Frame = 1

Query: 4   PFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYIGI 63
           P RWLKML+++LH S++  V+   G++ GF  S   V +DYY KDVQK+QPS  Q    I
Sbjct: 30  PVRWLKMLASKLHWSYVFAVVSNCGINQGFGYSLGHVATDYYMKDVQKVQPSQYQALSAI 89

Query: 64  YTIPWVM-KPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGLLI 123
             I W++ KP++G+LTD  P+ G+ RRPYF+++GV+G  + + ++++  L +  AL  + 
Sbjct: 90  TRISWIIFKPLFGILTDVLPIFGFHRRPYFILAGVIGVVSLLFISLQSNLHLYLALFWMT 149

Query: 124 GISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLGAQ 183
             S AMAIADVTIDAC   NS +   LA D+QSLC    SIG L+G+  SG  VHL+G++
Sbjct: 150 ISSAAMAIADVTIDACTTYNSNKHPSLASDMQSLCSLSYSIGELLGFFMSGILVHLVGSK 209

Query: 184 G---------GSIKQVGV------------------AIRDTCKAIKYPHVWRPSLFMFLC 243
           G           +  VGV                  A++   + IK   VW+PSL+MF+ 
Sbjct: 210 GVFGLLTFTFALVSVVGVLFSEPRVHGFSFKQNFTNAMKAMWRTIKCSDVWQPSLYMFIT 269

Query: 244 LSLSISTHEGQFYWYTDKKAGPAFSQ-------------------------ESVGLIYAV 303
            +L +   EG FYW+TD K GP F+Q                         E+VG I ++
Sbjct: 270 RALGLDIKEGLFYWFTDSKDGPLFAQVIPKQVASIFSKSTNMLKAFLVCFQETVGFILSI 329

Query: 304 GSAASLIGVLIYHKTLRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVI 363
           GS  S++GVL+Y+  L+D  FR++  + QL + ++G  D+I VLR NL   +PD LF+V+
Sbjct: 330 GSIGSILGVLLYNLRLKDHPFRKLFLWTQLLFALSGMFDLILVLRLNLKFGLPDYLFIVV 389

Query: 364 EECVTRIVTRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGV 423
           +  V++++ R+ W  + VLNT+LC  GIEGTFFALL  ID+ G + S W GG +LH   V
Sbjct: 390 DGIVSKMIIRLTWMVIFVLNTKLCPHGIEGTFFALLMSIDNAGLMTSSWLGGKMLHVLKV 449

Query: 424 TRSDFRNLWLVVLLRTVLRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLV 433
           TR++F NLWL VL+R V+R + + F+FLVP  +Q    +P +  M   S  E   N+ L 
Sbjct: 450 TRTEFGNLWLAVLVRNVMRLLPICFLFLVPQGDQNTFKLPAE-IMGEDSEEEGTRNLELA 509

BLAST of Cucsa.162860.2 vs. TAIR10
Match: AT2G32040.1 (AT2G32040.1 Major facilitator superfamily protein)

HSP 1 Score: 106.3 bits (264), Expect = 4.9e-23
Identity = 70/222 (31.53%), Postives = 116/222 (52.25%), Query Frame = 1

Query: 199 AIKYPHVWRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIG 258
           AIK P+V+ P+LF+FL  +   S  +   +++T  K G  F+ E +G +  V S ASL+G
Sbjct: 344 AIKQPNVFLPTLFIFLWQATPHS--DSAMFYFTTNKLG--FTPEFLGRVKLVTSIASLLG 403

Query: 259 VLIYHKTLRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIV 318
           V +Y+  L+ +  R+I     +F    G   VI V  +N  L I DE F + +  +  ++
Sbjct: 404 VGLYNGFLKTVPLRKIFLVTTIFGTGLGMTQVILVSGFNRQLGISDEWFAIGDSLILTVL 463

Query: 319 TRIRWTPMMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNL 378
            +  + P++VL  RLC  G+E T FA L  I + G++     G  +  AFG+TR  F NL
Sbjct: 464 AQASFMPVLVLAARLCPEGMEATLFATLMSISNGGSVLGGLMGAGLTQAFGITRDSFGNL 523

Query: 379 WLVVLLRTVLRFVVVGFVFLVPDANQTDILIPGDHTMVRKSS 421
             +++L  +   + +  + L+P  +  D L   D  +  KS+
Sbjct: 524 STLIILCNLSSLLPLPLLGLLP-RDSPDTLTKDDADVEMKSN 560


HSP 2 Score: 84.7 bits (208), Expect = 1.5e-16
Identity = 59/176 (33.52%), Postives = 96/176 (54.55%), Query Frame = 1

Query: 8   LKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYIGIYTIP 67
           +K    EL P  +A V ++Y +  G  G     VS +Y KD   L P+   +  G+ ++P
Sbjct: 116 IKFYGVELSPDNVA-VAMVYFVQ-GVLGLARLAVS-FYLKDDLHLDPAETAVITGLSSLP 175

Query: 68  WVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGLLIGISGA 127
           W++KP++G ++D+ P+ GY RR Y V+SG++GA +   MA         A  +L+G S +
Sbjct: 176 WLVKPLYGFISDSVPLFGYRRRSYLVLSGLLGAFSWSLMAGFVDSKYSAAFCILLG-SLS 235

Query: 128 MAIADVTIDACIV-RNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLGAQ 183
           +A +DV +D+ +V R   E Q ++  LQSLC   S+ G ++    SG  V   G +
Sbjct: 236 VAFSDVVVDSMVVERARGESQSVSGSLQSLCWGSSAFGGIVSSYFSGSLVESYGVR 287

BLAST of Cucsa.162860.2 vs. NCBI nr
Match: gi|778672667|ref|XP_011649848.1| (PREDICTED: probable folate-biopterin transporter 6 [Cucumis sativus])

HSP 1 Score: 860.9 bits (2223), Expect = 9.6e-247
Identity = 436/473 (92.18%), Postives = 438/473 (92.60%), Query Frame = 1

Query: 1   MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY 60
           MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY
Sbjct: 1   MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY 60

Query: 61  IGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGL 120
           IGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGL
Sbjct: 61  IGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGL 120

Query: 121 LIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLG 180
           LIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLG
Sbjct: 121 LIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLG 180

Query: 181 -----------------------------------AQGGSIKQVGVAIRDTCKAIKYPHV 240
                                              ++GGSIKQVGVAIRDTCKAIKYPHV
Sbjct: 181 AQEALGILAIPPALVVLLGFFIHETPSPTLQYNGKSKGGSIKQVGVAIRDTCKAIKYPHV 240

Query: 241 WRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT 300
           WRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT
Sbjct: 241 WRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT 300

Query: 301 LRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTP 360
           LRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTP
Sbjct: 301 LRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTP 360

Query: 361 MMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLR 420
           MMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLR
Sbjct: 361 MMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLR 420

Query: 421 TVLRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLVSMKSEGRD 439
           TVLRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLVSMKSEGRD
Sbjct: 421 TVLRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLVSMKSEGRD 473

BLAST of Cucsa.162860.2 vs. NCBI nr
Match: gi|700207879|gb|KGN62998.1| (hypothetical protein Csa_2G382710 [Cucumis sativus])

HSP 1 Score: 860.9 bits (2223), Expect = 9.6e-247
Identity = 436/473 (92.18%), Postives = 438/473 (92.60%), Query Frame = 1

Query: 1   MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY 60
           MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY
Sbjct: 73  MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY 132

Query: 61  IGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGL 120
           IGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGL
Sbjct: 133 IGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGL 192

Query: 121 LIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLG 180
           LIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLG
Sbjct: 193 LIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLG 252

Query: 181 -----------------------------------AQGGSIKQVGVAIRDTCKAIKYPHV 240
                                              ++GGSIKQVGVAIRDTCKAIKYPHV
Sbjct: 253 AQEALGILAIPPALVVLLGFFIHETPSPTLQYNGKSKGGSIKQVGVAIRDTCKAIKYPHV 312

Query: 241 WRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT 300
           WRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT
Sbjct: 313 WRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT 372

Query: 301 LRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTP 360
           LRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTP
Sbjct: 373 LRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTP 432

Query: 361 MMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLR 420
           MMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLR
Sbjct: 433 MMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLR 492

Query: 421 TVLRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLVSMKSEGRD 439
           TVLRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLVSMKSEGRD
Sbjct: 493 TVLRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLVSMKSEGRD 545

BLAST of Cucsa.162860.2 vs. NCBI nr
Match: gi|659088992|ref|XP_008445270.1| (PREDICTED: probable folate-biopterin transporter 6 [Cucumis melo])

HSP 1 Score: 833.2 bits (2151), Expect = 2.1e-238
Identity = 423/477 (88.68%), Postives = 432/477 (90.57%), Query Frame = 1

Query: 1   MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY 60
           MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY
Sbjct: 24  MLQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIY 83

Query: 61  IGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGL 120
            GIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVG+AVKGGLGVLEALGL
Sbjct: 84  TGIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGVAVKGGLGVLEALGL 143

Query: 121 LIGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLG 180
           LIG+SGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYS+SG FVHLLG
Sbjct: 144 LIGVSGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSSSGIFVHLLG 203

Query: 181 AQ-----------------------------------GGSIKQVGVAIRDTCKAIKYPHV 240
           AQ                                   GG I QVGVAIR+TCKA+KYPHV
Sbjct: 204 AQEALGILAIPPALIILLGFFIHETPSPTLQYNGKSKGGCINQVGVAIRETCKAVKYPHV 263

Query: 241 WRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT 300
           WRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT
Sbjct: 264 WRPSLFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKT 323

Query: 301 LRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTP 360
           LRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLAL+IPDELFVVIEECVTRIVTRIRWTP
Sbjct: 324 LRDLKFRRILFFAQLFYGVAGFLDVIFVLRWNLALKIPDELFVVIEECVTRIVTRIRWTP 383

Query: 361 MMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLR 420
           MMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLR
Sbjct: 384 MMVLNTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLR 443

Query: 421 TVLRFVVVGFVFLVPDANQTDILIPGDHTMVRKSSS----EDGDNIPLVSMKSEGRD 439
           TVLRFVVV FVFLVPDANQ+DILIPGD TMVRKS+S    EDGDNIPLVSM+SEGRD
Sbjct: 444 TVLRFVVVAFVFLVPDANQSDILIPGDPTMVRKSNSRTTKEDGDNIPLVSMRSEGRD 500

BLAST of Cucsa.162860.2 vs. NCBI nr
Match: gi|703130080|ref|XP_010104519.1| (hypothetical protein L484_025493 [Morus notabilis])

HSP 1 Score: 608.2 bits (1567), Expect = 1.1e-170
Identity = 296/468 (63.25%), Postives = 366/468 (78.21%), Query Frame = 1

Query: 2   LQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYI 61
           L+PFRWL+MLS++L P+F+AGV+L+YGL+ GFSGS+FKVVSDYYWKDVQK+QPS VQ+Y 
Sbjct: 39  LEPFRWLQMLSSQLSPTFVAGVVLVYGLNQGFSGSFFKVVSDYYWKDVQKVQPSAVQLYS 98

Query: 62  GIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGLL 121
           G+YTIPWVMKPIWGLLTD FPVRG+ RRPYFV++GVVGA +AV +A+ GG+    A+G L
Sbjct: 99  GLYTIPWVMKPIWGLLTDVFPVRGFRRRPYFVLAGVVGALSAVAVALDGGMSAAAAVGCL 158

Query: 122 IGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLGA 181
           +G+S A+AIADVTIDACI RNSIE++ LA DLQSLCG CSS+G L+GYS+SGFFVH LG 
Sbjct: 159 MGLSAAVAIADVTIDACIARNSIEIRALASDLQSLCGFCSSVGALLGYSSSGFFVHHLGP 218

Query: 182 QGG-------------------------------SIKQVGVAIRDTCKAIKYPHVWRPSL 241
           QG                                +++++GVA++   K IK+P VW+PSL
Sbjct: 219 QGALGLLAVPPALLVVLGFFIYEQRTISSHSEKKAVEKLGVAMKGMYKTIKFPQVWKPSL 278

Query: 242 FMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDLK 301
           +M+L L+LSISTHEGQFYWYTD KAGPAFSQE VG+IYA+G+ AS++GVLIYHKTL+D  
Sbjct: 279 YMYLSLALSISTHEGQFYWYTDPKAGPAFSQEFVGIIYAIGAMASIVGVLIYHKTLKDHP 338

Query: 302 FRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTPMMVLN 361
           FR ILF AQL YGV+G +D+IF+LRWNLAL IPD  FVV+EECV RIV+RIRW PM+VL+
Sbjct: 339 FRNILFLAQLLYGVSGMIDLIFILRWNLALGIPDYFFVVMEECVHRIVSRIRWMPMIVLS 398

Query: 362 TRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRTVLRF 421
           +RLC +GIEGTFFALL CIDSLG L SKW GG+VLH   VTR+DF NLWLVVL+R VLRF
Sbjct: 399 SRLCPLGIEGTFFALLMCIDSLGGLSSKWGGGMVLHLLHVTRTDFTNLWLVVLIRNVLRF 458

Query: 422 VVVGFVFLVPDANQTDILIPGDHTMVRKSSS---EDGDNIPLVSMKSE 436
             + F+FLVP A Q+D+LIP D  + + S+S   ED +N+ LV +  E
Sbjct: 459 STLAFIFLVPKAGQSDVLIPSD-LLTKNSTSIDEEDHENLELVPLNVE 505

BLAST of Cucsa.162860.2 vs. NCBI nr
Match: gi|590571814|ref|XP_007011697.1| (Major facilitator superfamily protein [Theobroma cacao])

HSP 1 Score: 594.0 bits (1530), Expect = 2.2e-166
Identity = 285/462 (61.69%), Postives = 355/462 (76.84%), Query Frame = 1

Query: 2   LQPFRWLKMLSTELHPSFIAGVILIYGLSHGFSGSYFKVVSDYYWKDVQKLQPSVVQIYI 61
           L+PF+WL+MLS++L+P+FI G++L+YGLS GFS S+FKVV+DYYWKDVQK+QPSVVQ+YI
Sbjct: 35  LEPFQWLQMLSSQLNPTFIFGIVLVYGLSQGFSSSFFKVVTDYYWKDVQKVQPSVVQLYI 94

Query: 62  GIYTIPWVMKPIWGLLTDAFPVRGYLRRPYFVISGVVGATAAVGMAVKGGLGVLEALGLL 121
           G+Y IPW+MKP+WGL TD FP++GY RRPYFV++GVVG  +A+ +A+ G L    ALG L
Sbjct: 95  GLYYIPWIMKPVWGLFTDVFPIKGYRRRPYFVLAGVVGGASALMVALFGNLPAALALGCL 154

Query: 122 IGISGAMAIADVTIDACIVRNSIEVQWLAQDLQSLCGACSSIGHLIGYSTSGFFVHLLGA 181
           IG++  MAIADVTIDACI RNSI+V+ LA D+QSLCG CSS G LIGYSTSGFFVH LGA
Sbjct: 155 IGVAAGMAIADVTIDACIARNSIKVRSLAPDMQSLCGFCSSAGALIGYSTSGFFVHHLGA 214

Query: 182 QGGSI--------------------------------KQVGVAIRDTCKAIKYPHVWRPS 241
           QG  I                                + +GVA+R   + IK+P VW+PS
Sbjct: 215 QGALILLAIPPVFITVLGFVIYEMRSTSLQSEKQKAMETLGVALRGMYQTIKFPQVWKPS 274

Query: 242 LFMFLCLSLSISTHEGQFYWYTDKKAGPAFSQESVGLIYAVGSAASLIGVLIYHKTLRDL 301
           L+M+L L+LSISTHEGQFYWYTD KAGPAFSQE VG+IYA+G+ AS++GVLIYHKTL+D+
Sbjct: 275 LYMYLSLALSISTHEGQFYWYTDPKAGPAFSQEFVGVIYAIGAMASMVGVLIYHKTLKDI 334

Query: 302 KFRRILFFAQLFYGVAGFLDVIFVLRWNLALRIPDELFVVIEECVTRIVTRIRWTPMMVL 361
            FR +LFFAQL YG +G LD+IF+LRWNL L IPD  FV++EECV+RI++RIRW PM+VL
Sbjct: 335 PFRNLLFFAQLLYGTSGMLDLIFILRWNLVLGIPDYFFVIMEECVSRIISRIRWIPMIVL 394

Query: 362 NTRLCSVGIEGTFFALLACIDSLGTLCSKWSGGLVLHAFGVTRSDFRNLWLVVLLRTVLR 421
           +TRLC +GIEGTFFALL CIDSLG+L SKW GG+VLH   VTR+DF NLWLVVL+R  LR
Sbjct: 395 STRLCPLGIEGTFFALLMCIDSLGSLTSKWGGGIVLHTLHVTRTDFTNLWLVVLIRNTLR 454

Query: 422 FVVVGFVFLVPDANQTDILIPGDHTMVRKSSSEDGDNIPLVS 432
           F  +G +FLVP A+Q+D LIP D      + + D + + LVS
Sbjct: 455 FATLGLIFLVPKADQSDALIPPDILTNNSTVTADDEGLELVS 496

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
FBT6_ARATH8.3e-15857.45Probable folate-biopterin transporter 6 OS=Arabidopsis thaliana GN=At5g10820 PE=... [more]
FBT3_ARATH3.5e-11644.06Probable folate-biopterin transporter 3 OS=Arabidopsis thaliana GN=At1g79710 PE=... [more]
FBT2_ARATH4.9e-11043.84Probable folate-biopterin transporter 2 OS=Arabidopsis thaliana GN=At5g25050 PE=... [more]
FBT5_ARATH2.5e-10142.45Probable folate-biopterin transporter 5 OS=Arabidopsis thaliana GN=At5g25040 PE=... [more]
FBT_SYNY31.2e-3628.47Folate-biopterin transporter OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=... [more]
Match NameE-valueIdentityDescription
A0A0A0LMJ9_CUCSA6.7e-24792.18Uncharacterized protein OS=Cucumis sativus GN=Csa_2G382710 PE=4 SV=1[more]
W9RP11_9ROSA7.8e-17163.25Uncharacterized protein OS=Morus notabilis GN=L484_025493 PE=4 SV=1[more]
A0A061GIF0_THECC1.5e-16661.69Major facilitator superfamily protein OS=Theobroma cacao GN=TCM_036893 PE=4 SV=1[more]
A0A0D2UH96_GOSRA3.2e-16460.74Uncharacterized protein OS=Gossypium raimondii GN=B456_009G063700 PE=4 SV=1[more]
D7SV54_VITVI3.3e-16160.04Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0008g06790 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT5G10820.14.7e-15957.45 Major facilitator superfamily protein[more]
AT1G79710.12.0e-11744.06 Major facilitator superfamily protein[more]
AT5G25050.12.8e-11143.84 Major facilitator superfamily protein[more]
AT5G25040.22.1e-9840.25 Major facilitator superfamily protein[more]
AT2G32040.14.9e-2331.53 Major facilitator superfamily protein[more]
Match NameE-valueIdentityDescription
gi|778672667|ref|XP_011649848.1|9.6e-24792.18PREDICTED: probable folate-biopterin transporter 6 [Cucumis sativus][more]
gi|700207879|gb|KGN62998.1|9.6e-24792.18hypothetical protein Csa_2G382710 [Cucumis sativus][more]
gi|659088992|ref|XP_008445270.1|2.1e-23888.68PREDICTED: probable folate-biopterin transporter 6 [Cucumis melo][more]
gi|703130080|ref|XP_010104519.1|1.1e-17063.25hypothetical protein L484_025493 [Morus notabilis][more]
gi|590571814|ref|XP_007011697.1|2.2e-16661.69Major facilitator superfamily protein [Theobroma cacao][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR004324FBT
IPR020846MFS_dom
Vocabulary: Biological Process
TermDefinition
GO:0006810transport
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006810 transport
biological_process GO:0016132 brassinosteroid biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.162860Cucsa.162860gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.162860.2Cucsa.162860.2-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.162860.2.CDS.1Cucsa.162860.2.CDS.1CDS
Cucsa.162860.2.CDS.2Cucsa.162860.2.CDS.2CDS
Cucsa.162860.2.CDS.3Cucsa.162860.2.CDS.3CDS


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004324Folate-biopterin transporterPFAMPF03092BT1coord: 213..406
score: 6.7E-44coord: 39..182
score: 4.0
IPR020846Major facilitator superfamily domainunknownSSF103473MFS general substrate transportercoord: 23..397
score: 1.31
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 24..183
score: 7.0E-4coord: 205..396
score: 9.
NoneNo IPR availablePANTHERPTHR31585FAMILY NOT NAMEDcoord: 1..437
score: 7.1E
NoneNo IPR availablePANTHERPTHR31585:SF3FOLATE-BIOPTERIN TRANSPORTER 6-RELATEDcoord: 1..437
score: 7.1E