Cucsa.161260.1 (mRNA) Cucumber (Gy14) v1

NameCucsa.161260.1
TypemRNA
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionRegulator of chromosome condensation (RCC1) family protein
Locationscaffold01144 : 1157131 .. 1160200 (+)
Sequence length2241
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGATGAACTAGATCAACCAGTTATAGAAGTGAATTTTGTGTTATTCAATCAACCGATCCAATCTTCGTTTTCATCTCGAGCTCAACTTCTGATAGCACCATTAGCAAAGACGCCGTAGCTCTGTGCGTTACGTCCTTTGGTAAGAAAGGCGATGGAGGAGTCCGGAACTCGACCTTCTTCCGGTAAGTTGTCTGGTAAAGTCGTCGCAATTTCCGCCGGTGAAGCTCACACTCTCGTTCTCACAGGTAAGAGAGGACGATGGAGGTAGGGAGAGTTTCTGATTTGTACATTTGTGCATTTCTGTTGTTTCGAAGTTAACGGAGAACGAATCGGAGCTGGTGACGGAGTTTTGGTGTGGCTTGTAGGAGATGGCAGAGTGTATTCATGGGGAAGGGgAATGTTTGGGCGGCTCGGGACGGGTGCGGAATCGGATGAGCTGTTCCCCGTTCGAGTTAAGCTTGGTTCGGGGGAAGAGAATCTCAAAGTTGTGGCGATTGCTGCTGGTTCTTATCATAGCCTCGCTCTTACTGGTTAGTGTGCTTGTTTTTCTTTTCGGATATCAAATGTTGAATTCGATAGAGCTTTCTTTGCACTAACAATTGCCAAATTGTTCGGGTAAATGGAGACGATAGTATTAATTCTTTGCAGTATCTAAATTCTAAATCTAATACACGAGCATCCTATGATTCATTCTCTTGAAATATCCGATGATTTTtTtGTTCCTCGACGAAGAAGCTCGAAAAGTTTGATTCGCGTTCATTTTTAATGGCTTAGAGATATTTGTTCAACTGGTTGAGAACGTGAGCAATGTtATTTAAAGAAGAAACTGAGATAAAACTTTGAAGTAGATCTTTTATGAAATGGAGTGTTGAACTCAACGTACCATTCAAGAAACTAATGGCATAAACCAGAATGAATCTTATAAGTATGACCGAAGTATAATTTAAACTAAAATTTCAACACTTAGCCATGAAGACAGAACACTGAAAAGTATTCCCAGGTGCTACTTTACTGATTTGCAAATTCTCACCCTCAATTTATTCGCAGATGATGGTTCGGTTTGGTGCTGGGGTTACAATATCTGTATCCTTTGAAATGCATTCAGAGGAGAAATTATTAAGCATTCTTACACTATAGCTAGAGAGCATTTCATTTTTCCTTCTTACTGCATATGCCCTTTTTCATCCTTAACTATCATAAAGATGGCCAACTTGGATTTGATGGCGGGAATTCCGCCACCCCCTGCTTGCTGAAGCAGTTCCTTGAGTTGGAACCTCCAGAATCTTTGACAGAAGAATCAGGCTCAAAGAGTAAGGGATCACTAAAGGTCTGTTGGTTGGTTCTTTCCTTGTTTTGATTAATGGATGCTGAACTTTGCTTCTTTGTTTTTGGTTTTGAAGTTTCCATTGCTTCTATATCAGATTTGTGACATCAAAGCTGGGGGGCAGATTTCTTTTGCAATTGACGATCTTGGAGCACTATGGATGTGGGGTAATTGTCCAAAACCAAGCGGTGAGAATGAAGTTGGCTTATCTTATGTTAGCAGCTTCACACCCTCTCCAGTGTGGGAGTTCCATGGCCATACCGTTGTTAAGGTTGCTTGTGGAAATGAGCATATTGTGGCTCTAGTTAGTGTTGGGGAAACCTACAATAAAGCTGAAGACTTAGTTTGCTACTCCTGGGGAGATAATTCTCACGGTCAATTAGGCCTGGGAGATAAAGATAGCAGAGCATGCCCCCAAATTATCGAGACATTTAATGAGGCTTCCCCTTGGGCAGTGTACGAAGTAGCATGCGGGGCATTCCATACTGCTTTACTAAGTCACAGTAAGAGACCATGTGACACCTTAGAAAGTGTGTGTTGGACATTTGGTCTGGGGGAAAACGGACAGCTTGGGCATGGCACCACCCAAAATGAATTCCTTCCTCAGCCTGTAAAAGAGTTGCCACAATCTGTATCTCTGATCTCAGTCGCTTGTGGCCTATTTCATACAAGTGTTGTTTCATCAGGTGGAGATCTGTGGTCTTGGGGAATGGAAAGGGGACTTGGCCTTTGTCCAGATGCTAGTTTTACTGGAACCAACACGGGCGATGCTATATCTCCCCTATCCATCTTCTGTAGTGGCACTGAATCACCAAAATTTTTGGATCCTGTTCAAGTTGCTTGTGGGGCTGCTCATACTGTTATAGTTGCACATGATGGATATAAATTGTGGTCGTGGGGACGAGGAAGAAGCGGGGTTCTTGGTAATGGCAAGGCAGTCGACTGTTTTACTCCCACAGTTGTGTTGTGGCCTCCACTTAATGAGGATTTCGATCAAGAGGAAACTGAAACCAAATGCGAGGAAGATAAAGTTGAAGAAAATAAAACCGAGGAAGATAAAGTTGAAGAAAATAAAATCGAGGAAGTAACCGAAGAAAACAAAAAGCTGTTAATGGCTATGGAGGAAATAAAACTTCTTCTAGATAAATTATCTATCATGGAACGGTATGCAAGCTTTCTTCATGGCTCAATATTTGGAAAACCTTTCCAAGAGGGAGATATCCCACTATCATTGCGAAATTCTGGTTCCTTCGACATTGCTAAGGAATGGGAAAGCATGTTGGAGTCATCAGATCGATCAAATCTTTTAAGGTTGGAGACGGTTTATCAAAATATGCTGGCAAGTATTAAAGACAAGATATTGAAGAAAAGGGTCCAGGAGCTTGTGAAGGAGTTTCTTCAATCAGCAACACCCCCAAATTAGTGTCTCAGACGAACTGTTAATCAGGTATCAAAAAACCGAACATTTTCCAGCTATTCGAATTTTCAAATTTGTCATCGAAGATTATCAGTACTATTATAAATGGAAGGAAATGCATGGAGATGAGGGCCCGCTGAAGCTGAAGCTAAATATTACTGAACAAATATCCTTTTTTGGACCAGAGGTTATGCTTTGTAGAATTGGAATTGCATGTATGTAACAAGTAATAGGAATGCTAAGTTTATTGGACTTATTTTAAGTCAAATAGTTAGTCCTAAATTGTCCCATGTGGCGTTTTAGGTATCTACTTTTTGAGAGATTGGAGT

mRNA sequence

TGATGAACTAGATCAACCAGTTATAGAAGTGAATTTTGTGTTATTCAATCAACCGATCCAATCTTCGTTTTCATCTCGAGCTCAACTTCTGATAGCACCATTAGCAAAGACGCCGTAGCTCTGTGCGTTACGTCCTTTGGTAAGAAAGGCGATGGAGGAGTCCGGAACTCGACCTTCTTCCGGTAAGTTGTCTGGTAAAGTCGTCGCAATTTCCGCCGGTGAAGCTCACACTCTCGTTCTCACAGGAGATGGCAGAGTGTATTCATGGGGAAGGGGAATGTTTGGGCGGCTCGGGACGGGTGCGGAATCGGATGAGCTGTTCCCCGTTCGAGTTAAGCTTGGTTCGGGGGAAGAGAATCTCAAAGTTGTGGCGATTGCTGCTGGTTCTTATCATAGCCTCGCTCTTACTGATGATGGTTCGGTTTGGTGCTGGGGTTACAATATCTATGGCCAACTTGGATTTGATGGCGGGAATTCCGCCACCCCCTGCTTGCTGAAGCAGTTCCTTGAGTTGGAACCTCCAGAATCTTTGACAGAAGAATCAGGCTCAAAGAGTAAGGGATCACTAAAGTTTCCATTGCTTCTATATCAGATTTGTGACATCAAAGCTGGGGGGCAGATTTCTTTTGCAATTGACGATCTTGGAGCACTATGGATGTGGGGTAATTGTCCAAAACCAAGCGGTGAGAATGAAGTTGGCTTATCTTATGTTAGCAGCTTCACACCCTCTCCAGTGTGGGAGTTCCATGGCCATACCGTTGTTAAGGTTGCTTGTGGAAATGAGCATATTGTGGCTCTAGTTAGTGTTGGGGAAACCTACAATAAAGCTGAAGACTTAGTTTGCTACTCCTGGGGAGATAATTCTCACGGTCAATTAGGCCTGGGAGATAAAGATAGCAGAGCATGCCCCCAAATTATCGAGACATTTAATGAGGCTTCCCCTTGGGCAGTGTACGAAGTAGCATGCGGGGCATTCCATACTGCTTTACTAAGTCACAGTAAGAGACCATGTGACACCTTAGAAAGTGTGTGTTGGACATTTGGTCTGGGGGAAAACGGACAGCTTGGGCATGGCACCACCCAAAATGAATTCCTTCCTCAGCCTGTAAAAGAGTTGCCACAATCTGTATCTCTGATCTCAGTCGCTTGTGGCCTATTTCATACAAGTGTTGTTTCATCAGGTGGAGATCTGTGGTCTTGGGGAATGGAAAGGGGACTTGGCCTTTGTCCAGATGCTAGTTTTACTGGAACCAACACGGGCGATGCTATATCTCCCCTATCCATCTTCTGTAGTGGCACTGAATCACCAAAATTTTTGGATCCTGTTCAAGTTGCTTGTGGGGCTGCTCATACTGTTATAGTTGCACATGATGGATATAAATTGTGGTCGTGGGGACGAGGAAGAAGCGGGGTTCTTGGTAATGGCAAGGCAGTCGACTGTTTTACTCCCACAGTTGTGTTGTGGCCTCCACTTAATGAGGATTTCGATCAAGAGGAAACTGAAACCAAATGCGAGGaagataaagttgaagaaaataaaaccgaggaagataaagttgaagaaaataaaatCGAGGAAGTAACCGAAGAAAACAAAAAGCTGTTAATGGCTATGGAGGAAATAAAACTTCTTCTAGATAAATTATCTATCATGGAACGGTATGCAAGCTTTCTTCATGGCTCAATATTTGGAAAACCTTTCCAAGAGGGAGATATCCCACTATCATTGCGAAATTCTGGTTCCTTCGACATTGCTAAGGAATGGGAAAGCATGTTGGAGTCATCAGATCGATCAAATCTTTTAAGGTTGGAGACGGTTTATCAAAATATGCTGGCAAGTATTAAAGACAAGATATTGAAGAAAAGGGTCCAGGAGCTTGTGAAGGAGTTTCTTCAATCAGCAACACCCCCAAATTAGTGTCTCAGACGAACTGTTAATCAGGTATCAAAAAACCGAACATTTTCCAGCTATTCGAATTTTCAAATTTGTCATCGAAGATTATCAGTACTATTATAAATGGAAGGAAATGCATGGAGATGAGGGCCCGCTGAAGCTGAAGCTAAATATTACTGAACAAATATCCTTTTTTGGACCAGAGGTTATGCTTTGTAGAATTGGAATTGCATGTATGTAACAAGTAATAGGAATGCTAAGTTTATTGGACTTATTTTAAGTCAAATAGTTAGTCCTAAATTGTCCCATGTGGCGTTTTAGGTATCTACTTTTTGAGAGATTGGAGT

Coding sequence (CDS)

ATGGAGGAGTCCGGAACTCGACCTTCTTCCGGTAAGTTGTCTGGTAAAGTCGTCGCAATTTCCGCCGGTGAAGCTCACACTCTCGTTCTCACAGGAGATGGCAGAGTGTATTCATGGGGAAGGGgAATGTTTGGGCGGCTCGGGACGGGTGCGGAATCGGATGAGCTGTTCCCCGTTCGAGTTAAGCTTGGTTCGGGGGAAGAGAATCTCAAAGTTGTGGCGATTGCTGCTGGTTCTTATCATAGCCTCGCTCTTACTGATGATGGTTCGGTTTGGTGCTGGGGTTACAATATCTATGGCCAACTTGGATTTGATGGCGGGAATTCCGCCACCCCCTGCTTGCTGAAGCAGTTCCTTGAGTTGGAACCTCCAGAATCTTTGACAGAAGAATCAGGCTCAAAGAGTAAGGGATCACTAAAGTTTCCATTGCTTCTATATCAGATTTGTGACATCAAAGCTGGGGGGCAGATTTCTTTTGCAATTGACGATCTTGGAGCACTATGGATGTGGGGTAATTGTCCAAAACCAAGCGGTGAGAATGAAGTTGGCTTATCTTATGTTAGCAGCTTCACACCCTCTCCAGTGTGGGAGTTCCATGGCCATACCGTTGTTAAGGTTGCTTGTGGAAATGAGCATATTGTGGCTCTAGTTAGTGTTGGGGAAACCTACAATAAAGCTGAAGACTTAGTTTGCTACTCCTGGGGAGATAATTCTCACGGTCAATTAGGCCTGGGAGATAAAGATAGCAGAGCATGCCCCCAAATTATCGAGACATTTAATGAGGCTTCCCCTTGGGCAGTGTACGAAGTAGCATGCGGGGCATTCCATACTGCTTTACTAAGTCACAGTAAGAGACCATGTGACACCTTAGAAAGTGTGTGTTGGACATTTGGTCTGGGGGAAAACGGACAGCTTGGGCATGGCACCACCCAAAATGAATTCCTTCCTCAGCCTGTAAAAGAGTTGCCACAATCTGTATCTCTGATCTCAGTCGCTTGTGGCCTATTTCATACAAGTGTTGTTTCATCAGGTGGAGATCTGTGGTCTTGGGGAATGGAAAGGGGACTTGGCCTTTGTCCAGATGCTAGTTTTACTGGAACCAACACGGGCGATGCTATATCTCCCCTATCCATCTTCTGTAGTGGCACTGAATCACCAAAATTTTTGGATCCTGTTCAAGTTGCTTGTGGGGCTGCTCATACTGTTATAGTTGCACATGATGGATATAAATTGTGGTCGTGGGGACGAGGAAGAAGCGGGGTTCTTGGTAATGGCAAGGCAGTCGACTGTTTTACTCCCACAGTTGTGTTGTGGCCTCCACTTAATGAGGATTTCGATCAAGAGGAAACTGAAACCAAATGCGAGGAAGATAAAGTTGAAGAAAATAAAACCGAGGAAGATAAAGTTGAAGAAAATAAAATCGAGGAAGTAACCGAAGAAAACAAAAAGCTGTTAATGGCTATGGAGGAAATAAAACTTCTTCTAGATAAATTATCTATCATGGAACGGTATGCAAGCTTTCTTCATGGCTCAATATTTGGAAAACCTTTCCAAGAGGGAGATATCCCACTATCATTGCGAAATTCTGGTTCCTTCGACATTGCTAAGGAATGGGAAAGCATGTTGGAGTCATCAGATCGATCAAATCTTTTAAGGTTGGAGACGGTTTATCAAAATATGCTGGCAAGTATTAAAGACAAGATATTGAAGAAAAGGGTCCAGGAGCTTGTGAAGGAGTTTCTTCAATCAGCAACACCCCCAAATTAG

Protein sequence

MEESGTRPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSGEENLKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLELEPPESLTEESGSKSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVGLSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERGLGLCPDASFTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSGVLGNGKAVDCFTPTVVLWPPLNEDFDQEETETKCEEDKVEENKTEEDKVEENKIEEVTEENKKLLMAMEEIKLLLDKLSIMERYASFLHGSIFGKPFQEGDIPLSLRNSGSFDIAKEWESMLESSDRSNLLRLETVYQNMLASIKDKILKKRVQELVKEFLQSATPPN*
BLAST of Cucsa.161260.1 vs. Swiss-Prot
Match: UVR8_ARATH (Ultraviolet-B receptor UVR8 OS=Arabidopsis thaliana GN=UVR8 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.8e-35
Identity = 123/427 (28.81%), Postives = 189/427 (44.26%), Query Frame = 1

Query: 16  KVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSGEENLKVVAI 75
           KV+ ISAG +H++ L     V SWGRG  G+LG G   D   P ++    G +   +V++
Sbjct: 16  KVLIISAGASHSVALLSGDIVCSWGRGEDGQLGHGDAEDRPSPTQLSALDGHQ---IVSV 75

Query: 76  AAGSYHSLALTDDG-SVWCWGYNIYGQLGFDGGNSATPCLLKQFLELEPPESLTEESGSK 135
             G+ H++A +  G  V+ WG+  +G+LG   GNS+         +L  P  +    G +
Sbjct: 76  TCGADHTVAYSQSGMEVYSWGWGDFGRLGH--GNSS---------DLFTPLPIKALHGIR 135

Query: 136 SKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVGLSYV-SSFTPS 195
            K              I  G     A+   G +  WG     +   ++GL     S  P 
Sbjct: 136 IK-------------QIACGDSHCLAVTMEGEVQSWGR----NQNGQLGLGDTEDSLVPQ 195

Query: 196 PVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACP 255
            +  F G  +  VA G EH  A+   G+          Y WG   +G LGLGD+  R  P
Sbjct: 196 KIQAFEGIRIKMVAAGAEHTAAVTEDGD---------LYGWGWGRYGNLGLGDRTDRLVP 255

Query: 256 QIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHGTTQNE 315
              E         +  VACG  HT  +S+S           +T+G  + GQLGHG  ++ 
Sbjct: 256 ---ERVTSTGGEKMSMVACGWRHTISVSYS--------GALYTYGWSKYGQLGHGDLEDH 315

Query: 316 FLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMER----GLGLCPDASFTGTNT 375
            +P  ++ L  S  +  ++ G  HT  ++S G L+ WG  +    G+G          N 
Sbjct: 316 LIPHKLEALSNSF-ISQISGGWRHTMALTSDGKLYGWGWNKFGQVGVG----------NN 374

Query: 376 GDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSGVLGNGKAVD 435
            D  SP+ +       P     VQV+CG  HT+ V  +   +++WGRG +G LG G++VD
Sbjct: 376 LDQCSPVQV-----RFPDDQKVVQVSCGWRHTLAVT-ERNNVFAWGRGTNGQLGIGESVD 374

Query: 436 CFTPTVV 437
              P ++
Sbjct: 436 RNFPKII 374

BLAST of Cucsa.161260.1 vs. Swiss-Prot
Match: HERC4_MOUSE (Probable E3 ubiquitin-protein ligase HERC4 OS=Mus musculus GN=Herc4 PE=1 SV=2)

HSP 1 Score: 128.3 bits (321), Expect = 2.9e-28
Identity = 102/347 (29.39%), Postives = 149/347 (42.94%), Query Frame = 1

Query: 7   RPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSG 66
           R S   ++ KV  +  G  HT+ +  DG VY+ G    G+LG   E     P +V     
Sbjct: 26  RRSDFFVNKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGH--EKSRKKPEQVVALDA 85

Query: 67  EENLKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLELEPPES 126
           +    +VA+A G  H+LAL D G V+ WG +  GQLG  G              +  P +
Sbjct: 86  QN---IVAVACGEAHTLALNDKGQVYAWGLDSDGQLGLQGSEEC----------IRVPRN 145

Query: 127 LTEESGSKSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVGLSY 186
           +   S               QI  +  G   S A+     ++ WG      G+  +G+  
Sbjct: 146 IKSLSD-------------IQIVQVACGYYHSLALSKASEVFCWGQ--NKYGQLGLGIDC 205

Query: 187 VSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGD 246
               +P  +    G   ++VA G  H   L   G           + WG N  GQLGL D
Sbjct: 206 QKQTSPQLIKSLLGIPFMQVAAGGAHSFVLTLSG---------AIFGWGRNKFGQLGLND 265

Query: 247 KDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLG 306
           ++ R  P ++++        +  + CG  HTA L        T E   +TFG G  GQLG
Sbjct: 266 ENDRYVPNLLKSLRSQK---IVYICCGEDHTAAL--------TKEGGVFTFGAGGYGQLG 321

Query: 307 HGTTQNEFLPQPVKELPQSVSLISVACGLFHTSV-VSSGGDLWSWGM 353
           H +T +E  P+ V EL  S+ +  VACG  HTS  V S G ++S+G+
Sbjct: 326 HNSTSHEINPRKVFELMGSI-VTQVACGRQHTSAFVPSSGRIYSFGL 321


HSP 2 Score: 125.9 bits (315), Expect = 1.4e-27
Identity = 102/342 (29.82%), Postives = 149/342 (43.57%), Query Frame = 1

Query: 17  VVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGT-GAESDELFPVRVKLGSGEENLKVVAI 76
           +VA++ GEAHTL L   G+VY+WG    G+LG  G+E     P  +K  S   ++++V +
Sbjct: 86  IVAVACGEAHTLALNDKGQVYAWGLDSDGQLGLQGSEECIRVPRNIKSLS---DIQIVQV 145

Query: 77  AAGSYHSLALTDDGSVWCWGYNIYGQ--LGFDGGNSATPCLLKQFLELEPPESLTEESGS 136
           A G YHSLAL+    V+CWG N YGQ  LG D     +P L+K  L +            
Sbjct: 146 ACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCQKQTSPQLIKSLLGI------------ 205

Query: 137 KSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVGLSYVSS-FTP 196
                            + AGG  SF +   GA++ WG     +   ++GL+  +  + P
Sbjct: 206 -------------PFMQVAAGGAHSFVLTLSGAIFGWGR----NKFGQLGLNDENDRYVP 265

Query: 197 SPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRAC 256
           + +       +V + CG +H  AL   G           +++G   +GQLG         
Sbjct: 266 NLLKSLRSQKIVYICCGEDHTAALTKEGGV---------FTFGAGGYGQLGHNSTSHEIN 325

Query: 257 PQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHGTTQN 316
           P+ +    E     V +VACG  HT+    S           ++FGLG NGQLG G+T N
Sbjct: 326 PRKV---FELMGSIVTQVACGRQHTSAFVPS-------SGRIYSFGLGGNGQLGTGSTSN 372

Query: 317 EFLPQPVK--------ELPQSVSLISVACGLFHTSVVSSGGD 347
              P  VK        + PQ +      C       + SGGD
Sbjct: 386 RKSPFTVKGNWFSYNGQCPQDIGSEDYFC----VKRIFSGGD 372


HSP 3 Score: 85.5 bits (210), Expect = 2.1e-15
Identity = 94/349 (26.93%), Postives = 145/349 (41.55%), Query Frame = 1

Query: 93  CWGYNIYGQLGFDGGNSATPCLLKQFLELEPPESLTEESGSKSKGSLKFPLLLYQICDIK 152
           CWG   YGQLG  G        + + + LEP  S                 +  ++ D+ 
Sbjct: 3   CWGNASYGQLGLGG--------IDEEIVLEPRRSDF--------------FVNKKVRDVG 62

Query: 153 AGGQISFAIDDLGALWMWGNCPKPSGENEVG-LSYVSSFT-PSPVWEFHGHTVVKVACGN 212
            G + +  + D G ++  G C      N++G L +  S   P  V       +V VACG 
Sbjct: 63  CGLRHTVFVLDDGTVYTCG-C------NDLGQLGHEKSRKKPEQVVALDAQNIVAVACGE 122

Query: 213 EHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACPQIIETFNEASPWAVYEV 272
            H +AL   G+          Y+WG +S GQLGL  + S  C ++       S   + +V
Sbjct: 123 AHTLALNDKGQV---------YAWGLDSDGQLGL--QGSEECIRVPRNIKSLSDIQIVQV 182

Query: 273 ACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHG-TTQNEFLPQPVKELPQSVSLI 332
           ACG +H+  LS +       E  CW  G  + GQLG G   Q +  PQ +K L   +  +
Sbjct: 183 ACGYYHSLALSKAS------EVFCW--GQNKYGQLGLGIDCQKQTSPQLIKSL-LGIPFM 242

Query: 333 SVACGLFHTSVVSSGGDLWSWGMER--GLGLCPDASFTGTNTGDAISPLSIFCSGTESPK 392
            VA G  H+ V++  G ++ WG  +   LGL         +  D   P  +     +S +
Sbjct: 243 QVAAGGAHSFVLTLSGAIFGWGRNKFGQLGL--------NDENDRYVPNLL-----KSLR 288

Query: 393 FLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSGVLGNGKAVDCFTPTVV 437
               V + CG  HT  +  +G  ++++G G  G LG+        P  V
Sbjct: 303 SQKIVYICCGEDHTAALTKEG-GVFTFGAGGYGQLGHNSTSHEINPRKV 288

BLAST of Cucsa.161260.1 vs. Swiss-Prot
Match: HERC4_HUMAN (Probable E3 ubiquitin-protein ligase HERC4 OS=Homo sapiens GN=HERC4 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 3.7e-28
Identity = 97/308 (31.49%), Postives = 139/308 (45.13%), Query Frame = 1

Query: 17  VVAISAGEAHTLVLTGDGRVYSWGRGMFGRLG-TGAESDELFPVRVKLGSGEENLKVVAI 76
           +VA+S GEAHTL L   G+VY+WG    G+LG  G+E     P  +K  S   ++++V +
Sbjct: 86  IVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSLS---DIQIVQV 145

Query: 77  AAGSYHSLALTDDGSVWCWGYNIYGQLGF--DGGNSATPCLLKQFLELEPPESLTEESGS 136
           A G YHSLAL+    V+CWG N YGQLG   D     +P LLK  L +            
Sbjct: 146 ACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGI------------ 205

Query: 137 KSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVGLSYVSS-FTP 196
                            + AGG  SF +   GA++ WG     +   ++GL+  +  + P
Sbjct: 206 -------------PFMQVAAGGAHSFVLTLSGAIFGWGR----NKFGQLGLNDENDRYVP 265

Query: 197 SPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRAC 256
           + +       +V + CG +H  AL   G           +++G   +GQLG         
Sbjct: 266 NLLKSLRSQKIVYICCGEDHTAALTKEGGV---------FTFGAGGYGQLGHNSTSHEIN 325

Query: 257 PQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHGTTQN 316
           P+ +    E     V E+ACG  HT+    S           ++FGLG NGQLG G+T N
Sbjct: 326 PRKV---FELMGSIVTEIACGRQHTSAFVPS-------SGRIYSFGLGGNGQLGTGSTSN 342

Query: 317 EFLPQPVK 321
              P  VK
Sbjct: 386 RKSPFTVK 342


HSP 2 Score: 123.2 bits (308), Expect = 9.2e-27
Identity = 99/347 (28.53%), Postives = 148/347 (42.65%), Query Frame = 1

Query: 7   RPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSG 66
           R S   ++ +V  +  G  HT+ +  DG VY+ G    G+LG   E     P +V     
Sbjct: 26  RKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGH--EKSRKKPEQVVALDA 85

Query: 67  EENLKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLELEPPES 126
           +    +VA++ G  H+LAL D G V+ WG +  GQLG  G              +  P +
Sbjct: 86  QN---IVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEEC----------IRVPRN 145

Query: 127 LTEESGSKSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVGLSY 186
           +   S               QI  +  G   S A+     ++ WG      G+  +G   
Sbjct: 146 IKSLSD-------------IQIVQVACGYYHSLALSKASEVFCWGQ--NKYGQLGLGTDC 205

Query: 187 VSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGD 246
               +P  +    G   ++VA G  H   L   G           + WG N  GQLGL D
Sbjct: 206 KKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSG---------AIFGWGRNKFGQLGLND 265

Query: 247 KDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLG 306
           ++ R  P ++++        +  + CG  HTA L        T E   +TFG G  GQLG
Sbjct: 266 ENDRYVPNLLKSLRSQK---IVYICCGEDHTAAL--------TKEGGVFTFGAGGYGQLG 321

Query: 307 HGTTQNEFLPQPVKELPQSVSLISVACGLFHTSV-VSSGGDLWSWGM 353
           H +T +E  P+ V EL  S+ +  +ACG  HTS  V S G ++S+G+
Sbjct: 326 HNSTSHEINPRKVFELMGSI-VTEIACGRQHTSAFVPSSGRIYSFGL 321


HSP 3 Score: 82.4 bits (202), Expect = 1.8e-14
Identity = 92/349 (26.36%), Postives = 146/349 (41.83%), Query Frame = 1

Query: 93  CWGYNIYGQLGFDGGNSATPCLLKQFLELEPPESLTEESGSKSKGSLKFPLLLYQICDIK 152
           CWG   +GQLG  G        + + + LEP +S                 +  ++ D+ 
Sbjct: 3   CWGNASFGQLGLGG--------IDEEIVLEPRKSDF--------------FINKRVRDVG 62

Query: 153 AGGQISFAIDDLGALWMWGNCPKPSGENEVG-LSYVSSFT-PSPVWEFHGHTVVKVACGN 212
            G + +  + D G ++  G C      N++G L +  S   P  V       +V V+CG 
Sbjct: 63  CGLRHTVFVLDDGTVYTCG-C------NDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGE 122

Query: 213 EHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACPQIIETFNEASPWAVYEV 272
            H +AL   G+          Y+WG +S GQLGL    S  C ++       S   + +V
Sbjct: 123 AHTLALNDKGQV---------YAWGLDSDGQLGL--VGSEECIRVPRNIKSLSDIQIVQV 182

Query: 273 ACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHGT-TQNEFLPQPVKELPQSVSLI 332
           ACG +H+  LS +       E  CW  G  + GQLG GT  + +  PQ +K L   +  +
Sbjct: 183 ACGYYHSLALSKAS------EVFCW--GQNKYGQLGLGTDCKKQTSPQLLKSL-LGIPFM 242

Query: 333 SVACGLFHTSVVSSGGDLWSWGMER--GLGLCPDASFTGTNTGDAISPLSIFCSGTESPK 392
            VA G  H+ V++  G ++ WG  +   LGL         +  D   P  +     +S +
Sbjct: 243 QVAAGGAHSFVLTLSGAIFGWGRNKFGQLGL--------NDENDRYVPNLL-----KSLR 288

Query: 393 FLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSGVLGNGKAVDCFTPTVV 437
               V + CG  HT  +  +G  ++++G G  G LG+        P  V
Sbjct: 303 SQKIVYICCGEDHTAALTKEG-GVFTFGAGGYGQLGHNSTSHEINPRKV 288

BLAST of Cucsa.161260.1 vs. Swiss-Prot
Match: HERC4_RAT (Probable E3 ubiquitin-protein ligase HERC4 OS=Rattus norvegicus GN=Herc4 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 6.4e-28
Identity = 100/347 (28.82%), Postives = 149/347 (42.94%), Query Frame = 1

Query: 7   RPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSG 66
           R S   ++ KV  +  G  HT+ +  DG VY+ G    G+LG   E     P +V     
Sbjct: 26  RKSDFFVNKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGH--EKSRKKPEQVVALDA 85

Query: 67  EENLKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLELEPPES 126
           +    +VA++ G  H+LAL D G V+ WG +  GQLG  G              +  P +
Sbjct: 86  QN---IVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLQGSEEC----------IRVPRN 145

Query: 127 LTEESGSKSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVGLSY 186
           +   S               QI  +  G   S A+     ++ WG      G+  +G+  
Sbjct: 146 IKSLSD-------------IQIVQVACGYYHSLALSKASEVFCWGQ--NKYGQLGLGIEC 205

Query: 187 VSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGD 246
               +P  +    G   ++VA G  H   L   G           + WG N  GQLGL D
Sbjct: 206 QKQTSPQLIKSLLGIPFMQVAAGGAHSFVLTLSG---------AIFGWGRNKFGQLGLND 265

Query: 247 KDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLG 306
           ++ R  P ++++        +  + CG  HTA L        T E   +TFG G  GQLG
Sbjct: 266 ENDRYVPNLLKSLRSQK---IVYICCGEDHTAAL--------TKEGGVFTFGAGGYGQLG 321

Query: 307 HGTTQNEFLPQPVKELPQSVSLISVACGLFHTSV-VSSGGDLWSWGM 353
           H +T +E  P+ V EL  S+ +  +ACG  HTS  V S G ++S+G+
Sbjct: 326 HNSTSHEINPRKVFELMGSI-VTQIACGRQHTSAFVPSSGRIYSFGL 321


HSP 2 Score: 125.2 bits (313), Expect = 2.4e-27
Identity = 101/342 (29.53%), Postives = 149/342 (43.57%), Query Frame = 1

Query: 17  VVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGT-GAESDELFPVRVKLGSGEENLKVVAI 76
           +VA+S GEAHTL L   G+VY+WG    G+LG  G+E     P  +K  S   ++++V +
Sbjct: 86  IVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLQGSEECIRVPRNIKSLS---DIQIVQV 145

Query: 77  AAGSYHSLALTDDGSVWCWGYNIYGQ--LGFDGGNSATPCLLKQFLELEPPESLTEESGS 136
           A G YHSLAL+    V+CWG N YGQ  LG +     +P L+K  L +            
Sbjct: 146 ACGYYHSLALSKASEVFCWGQNKYGQLGLGIECQKQTSPQLIKSLLGI------------ 205

Query: 137 KSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVGLSYVSS-FTP 196
                            + AGG  SF +   GA++ WG     +   ++GL+  +  + P
Sbjct: 206 -------------PFMQVAAGGAHSFVLTLSGAIFGWGR----NKFGQLGLNDENDRYVP 265

Query: 197 SPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRAC 256
           + +       +V + CG +H  AL   G           +++G   +GQLG         
Sbjct: 266 NLLKSLRSQKIVYICCGEDHTAALTKEGGV---------FTFGAGGYGQLGHNSTSHEIN 325

Query: 257 PQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHGTTQN 316
           P+ +    E     V ++ACG  HT+    S           ++FGLG NGQLG G+T N
Sbjct: 326 PRKV---FELMGSIVTQIACGRQHTSAFVPS-------SGRIYSFGLGGNGQLGTGSTSN 372

Query: 317 EFLPQPVK--------ELPQSVSLISVACGLFHTSVVSSGGD 347
              P  VK        + PQ +      C       + SGGD
Sbjct: 386 RKSPFTVKGNWFSYNGQCPQDIGSEDYFC----VKRIFSGGD 372


HSP 3 Score: 84.7 bits (208), Expect = 3.6e-15
Identity = 93/349 (26.65%), Postives = 146/349 (41.83%), Query Frame = 1

Query: 93  CWGYNIYGQLGFDGGNSATPCLLKQFLELEPPESLTEESGSKSKGSLKFPLLLYQICDIK 152
           CWG   YGQLG  G        + + + LEP +S                 +  ++ D+ 
Sbjct: 3   CWGNASYGQLGLGG--------IDEEIVLEPRKSDF--------------FVNKKVRDVG 62

Query: 153 AGGQISFAIDDLGALWMWGNCPKPSGENEVG-LSYVSSFT-PSPVWEFHGHTVVKVACGN 212
            G + +  + D G ++  G C      N++G L +  S   P  V       +V V+CG 
Sbjct: 63  CGLRHTVFVLDDGTVYTCG-C------NDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGE 122

Query: 213 EHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACPQIIETFNEASPWAVYEV 272
            H +AL   G+          Y+WG +S GQLGL  + S  C ++       S   + +V
Sbjct: 123 AHTLALNDKGQV---------YAWGLDSDGQLGL--QGSEECIRVPRNIKSLSDIQIVQV 182

Query: 273 ACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHG-TTQNEFLPQPVKELPQSVSLI 332
           ACG +H+  LS +       E  CW  G  + GQLG G   Q +  PQ +K L   +  +
Sbjct: 183 ACGYYHSLALSKAS------EVFCW--GQNKYGQLGLGIECQKQTSPQLIKSL-LGIPFM 242

Query: 333 SVACGLFHTSVVSSGGDLWSWGMER--GLGLCPDASFTGTNTGDAISPLSIFCSGTESPK 392
            VA G  H+ V++  G ++ WG  +   LGL         +  D   P  +     +S +
Sbjct: 243 QVAAGGAHSFVLTLSGAIFGWGRNKFGQLGL--------NDENDRYVPNLL-----KSLR 288

Query: 393 FLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSGVLGNGKAVDCFTPTVV 437
               V + CG  HT  +  +G  ++++G G  G LG+        P  V
Sbjct: 303 SQKIVYICCGEDHTAALTKEG-GVFTFGAGGYGQLGHNSTSHEINPRKV 288

BLAST of Cucsa.161260.1 vs. Swiss-Prot
Match: HERC2_MOUSE (E3 ubiquitin-protein ligase HERC2 OS=Mus musculus GN=Herc2 PE=1 SV=3)

HSP 1 Score: 126.7 bits (317), Expect = 8.3e-28
Identity = 113/390 (28.97%), Postives = 165/390 (42.31%), Query Frame = 1

Query: 17   VVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSGEENLKVVAIA 76
            VV ++ G      +T +G+VYS G    GRLG G  S  +   R         +K VA+ 
Sbjct: 2995 VVQVAGGSKSLFAVTVEGKVYSCGEATNGRLGLGMSSGTVPIPRQITALSSYVVKKVAVH 3054

Query: 77   AGSYHSLALTDDGSVWCWGYNIYGQLG-FDGGNSATPCLLKQFLELEPPESLTEESGSKS 136
            +G  H+ ALT DG V+ WG    G+LG F   N   P L++                 K+
Sbjct: 3055 SGGRHATALTVDGKVFSWGEGDDGKLGHFSRMNCDKPRLIEAL---------------KT 3114

Query: 137  KGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWG---NCPKPSGENEVGLSYVSSFTP 196
            K          +I DI  G   S A+   G L+ WG         G+N   L       P
Sbjct: 3115 K----------RIRDIACGSSHSAALTSSGELYTWGLGEYGRLGHGDNTTQLK------P 3174

Query: 197  SPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRAC 256
              V    GH V++VACG+          +T    ++ + +SWGD   G+LG G  +    
Sbjct: 3175 KMVKVLLGHRVIQVACGSRD-------AQTLALTDEGLVFSWGDGDFGKLGRGGSEGCNI 3234

Query: 257  PQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHGTTQN 316
            PQ IE  N      V ++ CGA  +  L+ S         V WT+G G+  +LGHG+  +
Sbjct: 3235 PQNIERLNGQ---GVCQIECGAQFSLALTKS--------GVVWTWGKGDYFRLGHGSDVH 3294

Query: 317  EFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERGLGLCPDASFTGTNTGDA 376
               PQ V+ L +   ++ VA G  H   V+  G +++WG         D    G  T   
Sbjct: 3295 VRKPQVVEGL-RGKKIVHVAVGALHCLAVTDSGQVYAWGDN-------DHGQQGNGT-TT 3323

Query: 377  ISPLSIFCSGTESPKFLDPVQVACGAAHTV 403
            ++       G E  K     +VACG++H+V
Sbjct: 3355 VNRKPTLVQGLEGQKI---TRVACGSSHSV 3323


HSP 2 Score: 119.4 bits (298), Expect = 1.3e-25
Identity = 104/392 (26.53%), Postives = 166/392 (42.35%), Query Frame = 1

Query: 18   VAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSGEENLKVVAIAA 77
            V +  GE     +T DG++Y+ G G  GRLG G       P  ++       +K VA+ +
Sbjct: 3990 VQLIGGEQTLFAVTADGKLYATGYGAGGRLGIGGTESVSTPTLLE-SIQHVFIKKVAVNS 4049

Query: 78   GSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLELEPPESLTEESGSKSKG 137
            G  H LAL+ +G V+ WG    G+LG     + +PC   + +E                 
Sbjct: 4050 GGKHCLALSSEGEVYSWGEAEDGKLGH---GNRSPCDRPRVIE----------------- 4109

Query: 138  SLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVGLS-YVSSFTPSPVW 197
                 L   ++ D+ AGG  S  +   G L+ WG          +G S       P  V 
Sbjct: 4110 ----SLRGIEVVDVAAGGAHSACVTAAGDLYTWGK----GRYGRLGHSDSEDQLKPKLVE 4169

Query: 198  EFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACPQII 257
               GH V+ +ACG+          +T    +D   +SWGD  +G+LG G  D    P  I
Sbjct: 4170 ALQGHRVIDIACGS-------GDAQTLCLTDDDTVWSWGDGDYGKLGRGGSDGCKVPMKI 4229

Query: 258  ETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHGTTQNEFLP 317
            ++    +   V +V CG+  +  L+ S           +T+G G+  +LGHG+  +   P
Sbjct: 4230 DSL---TGLGVVKVECGSQFSVALTKS--------GAVYTWGKGDYHRLGHGSDDHVRRP 4289

Query: 318  QPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERGLGLCPDASFTGTNTGDAISPL 377
            + V+ L Q   +I++A G  H    +  G++++WG         D    G  T +AI   
Sbjct: 4290 RQVQGL-QGKKVIAIATGSLHCVCCTEDGEVYTWGDN-------DEGQLGDGTTNAI--- 4316

Query: 378  SIFCSGTESPKFLDPVQ------VACGAAHTV 403
                   + P+ +  +Q      VACG+AHT+
Sbjct: 4350 -------QRPRLVAALQGKKVNRVACGSAHTL 4316


HSP 3 Score: 106.3 bits (264), Expect = 1.2e-21
Identity = 75/238 (31.51%), Postives = 115/238 (48.32%), Query Frame = 1

Query: 10  SGKLSGK-VVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSGEE 69
           SGK +GK VV I+ G  ++  +T +G +Y+WGRG +GRLG G+  DE  P+ V   +G +
Sbjct: 546 SGKQAGKHVVHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDEAIPMLV---AGLK 605

Query: 70  NLKVVAIAAGS--YHSLALTDDGSVWCWGYNIYGQLGFDGGNSA-TPCLLKQFLELEPPE 129
            LKV+ +A GS    +LA+T++G VW WG   YG+LG  G +   TP L+++  +L+   
Sbjct: 606 GLKVIDVACGSGDAQTLAVTENGQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLD--- 665

Query: 130 SLTEESGSKSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGENE-VGL 189
                                 +  ++ G Q S A+   G ++ WG      G+N+ +G 
Sbjct: 666 ----------------------VIKVRCGSQFSIALTKDGQVYSWG-----KGDNQRLGH 725

Query: 190 SYVSSFT-PSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQ 242
                   P  +    G  V+ VA G+ H +AL          ED   +SWG N   Q
Sbjct: 726 GTEEHVRYPKLLEGLQGKKVIDVAAGSTHCLAL---------TEDSEVHSWGSNDQCQ 741


HSP 4 Score: 96.7 bits (239), Expect = 9.2e-19
Identity = 72/239 (30.13%), Postives = 116/239 (48.54%), Query Frame = 1

Query: 192 PSPVWEFHGHTVVKVACGNE--HIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDS 251
           P  V       +VK+A  ++  H +AL + GE Y         SWG    G+LG GD   
Sbjct: 485 PQLVQGLASRNIVKIAAHSDGHHYLALAATGEVY---------SWGCGDGGRLGHGDTVP 544

Query: 252 RACPQIIETFN-EASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHG 311
              P++I  F+ + +   V  +ACG+ ++A +        T E   +T+G G  G+LGHG
Sbjct: 545 LEEPKVISAFSGKQAGKHVVHIACGSTYSAAI--------TAEGELYTWGRGNYGRLGHG 604

Query: 312 TTQNEFLPQPVKELPQSVSLISVACGL--FHTSVVSSGGDLWSWGMERGLGLCPDASFTG 371
           ++++E +P  V  L + + +I VACG     T  V+  G +WSWG +   G        G
Sbjct: 605 SSEDEAIPMLVAGL-KGLKVIDVACGSGDAQTLAVTENGQVWSWG-DGDYGKLGRGGSDG 664

Query: 372 TNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSGVLGNG 426
             T   I  L          + LD ++V CG+  ++ +  DG +++SWG+G +  LG+G
Sbjct: 665 CKTPKLIEKL----------QDLDVIKVRCGSQFSIALTKDG-QVYSWGKGDNQRLGHG 693


HSP 5 Score: 96.3 bits (238), Expect = 1.2e-18
Identity = 97/342 (28.36%), Postives = 143/342 (41.81%), Query Frame = 1

Query: 16  KVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSGEENLK-VVA 75
           K+ A S G  H L L   G VYSWG G  GRLG G       P  +   SG++  K VV 
Sbjct: 498 KIAAHSDGH-HYLALAATGEVYSWGCGDGGRLGHGDTVPLEEPKVISAFSGKQAGKHVVH 557

Query: 76  IAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGG-NSATPCLLKQFLELEPPESLTEESGS 135
           IA GS +S A+T +G ++ WG   YG+LG     + A P L+                  
Sbjct: 558 IACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDEAIPMLV------------------ 617

Query: 136 KSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVGLSYVSSF-TP 195
                LK   ++   C   +G   + A+ + G +W WG+        ++G        TP
Sbjct: 618 ---AGLKGLKVIDVACG--SGDAQTLAVTENGQVWSWGD----GDYGKLGRGGSDGCKTP 677

Query: 196 SPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRAC 255
             + +     V+KV CG++  +AL   G+          YSWG   + +LG G ++    
Sbjct: 678 KLIEKLQDLDVIKVRCGSQFSIALTKDGQV---------YSWGKGDNQRLGHGTEEHVRY 737

Query: 256 PQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHGTTQN 315
           P+++E         V +VA G+ H   L+         E   W    G N Q  H  T  
Sbjct: 738 PKLLEGLQGKK---VIDVAAGSTHCLALTEDS------EVHSW----GSNDQCQHFDTLR 787

Query: 316 EFLPQPVKELP--QSVSLISVACGLFHTSVVSSGGDLWSWGM 353
              P+P   LP   S  ++ +ACG   +   SS  + WS G+
Sbjct: 798 VTKPEPT-ALPGLDSKHIVGIACGPAQSFAWSSCSE-WSIGL 787


HSP 6 Score: 87.4 bits (215), Expect = 5.6e-16
Identity = 87/327 (26.61%), Postives = 137/327 (41.90%), Query Frame = 1

Query: 121  LEPPESLTEESGSKSK-GSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGE 180
            L   + L    GSK K  S    L    +  +  G +  FA+   G ++  G     +G 
Sbjct: 2967 LNDKDQLGGLKGSKIKVPSFSETLSALNVVQVAGGSKSLFAVTVEGKVYSCGEAT--NGR 3026

Query: 181  NEVGLSYVSSFTPSPVWEFHGHTVVKVAC--GNEHIVALVSVGETYNKAEDLVCYSWGDN 240
              +G+S  +   P  +     + V KVA   G  H  AL   G+ +         SWG+ 
Sbjct: 3027 LGLGMSSGTVPIPRQITALSSYVVKKVAVHSGGRHATALTVDGKVF---------SWGEG 3086

Query: 241  SHGQLGLGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTF 300
              G+LG   + +   P++IE         + ++ACG+ H+A L+ S           +T+
Sbjct: 3087 DDGKLGHFSRMNCDKPRLIEALKTKR---IRDIACGSSHSAALTSSGE--------LYTW 3146

Query: 301  GLGENGQLGHGTTQNEFLPQPVKELPQSVSLISVACGL--FHTSVVSSGGDLWSWGMERG 360
            GLGE G+LGHG    +  P+ VK L     +I VACG     T  ++  G ++SWG    
Sbjct: 3147 GLGEYGRLGHGDNTTQLKPKMVKVL-LGHRVIQVACGSRDAQTLALTDEGLVFSWG---- 3206

Query: 361  LGLCPDASFTGTNTGDAISPLSIFCSGTESPKFLDPV------QVACGAAHTVIVAHDGY 420
                 D  F     G +         G   P+ ++ +      Q+ CGA  ++ +   G 
Sbjct: 3207 -----DGDFGKLGRGGS--------EGCNIPQNIERLNGQGVCQIECGAQFSLALTKSGV 3252

Query: 421  KLWSWGRGRSGVLGNGKAVDCFTPTVV 437
             +W+WG+G    LG+G  V    P VV
Sbjct: 3267 -VWTWGKGDYFRLGHGSDVHVRKPQVV 3252


HSP 7 Score: 85.1 bits (209), Expect = 2.8e-15
Identity = 71/257 (27.63%), Postives = 106/257 (41.25%), Query Frame = 1

Query: 13   LSGKVVAISAG--EAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSGEENL 72
            L  +V+ ++ G  +A TL LT +G V+SWG G FG+LG G       P  ++  +G+   
Sbjct: 3150 LGHRVIQVACGSRDAQTLALTDEGLVFSWGDGDFGKLGRGGSEGCNIPQNIERLNGQ--- 3209

Query: 73   KVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLELEPPESLTEE 132
             V  I  G+  SLALT  G VW WG   Y +LG               + +  P+ +   
Sbjct: 3210 GVCQIECGAQFSLALTKSGVVWTWGKGDYFRLGHGSD-----------VHVRKPQVVEGL 3269

Query: 133  SGSKSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVGLSYVSSF 192
             G K             I  +  G     A+ D G ++ WG+     G+   G + V+  
Sbjct: 3270 RGKK-------------IVHVAVGALHCLAVTDSGQVYAWGD--NDHGQQGNGTTTVNR- 3329

Query: 193  TPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLG---DK 252
             P+ V    G  + +VACG+ H VA  +V        + V +    +  G   LG   D 
Sbjct: 3330 KPTLVQGLEGQKITRVACGSSHSVAWTTVDVATPSVHEPVLFQTARDPLGASYLGVPSDA 3376

Query: 253  DSRACPQIIETFNEASP 265
            DS +    I   N   P
Sbjct: 3390 DSSSSSNKISGANNCKP 3376


HSP 8 Score: 74.3 bits (181), Expect = 4.9e-12
Identity = 56/216 (25.93%), Postives = 99/216 (45.83%), Query Frame = 1

Query: 200 GHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACPQIIETF 259
           G  VV +ACG+ +  A+ + GE Y         +WG  ++G+LG G  +  A P ++   
Sbjct: 551 GKHVVHIACGSTYSAAITAEGELY---------TWGRGNYGRLGHGSSEDEAIPMLVAGL 610

Query: 260 NEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHGTTQNEFLPQPV 319
                  V +VACG+     L+ ++          W++G G+ G+LG G +     P+ +
Sbjct: 611 KGLK---VIDVACGSGDAQTLAVTEN------GQVWSWGDGDYGKLGRGGSDGCKTPKLI 670

Query: 320 KELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERGLGLCPDASFTGTNTGDAISPLSIF 379
           ++L Q + +I V CG   +  ++  G ++SWG         D    G  T + +    + 
Sbjct: 671 EKL-QDLDVIKVRCGSQFSIALTKDGQVYSWGK-------GDNQRLGHGTEEHVRYPKLL 730

Query: 380 CSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWG 416
             G +  K +D   VA G+ H + +  D  ++ SWG
Sbjct: 731 -EGLQGKKVID---VAAGSTHCLALTEDS-EVHSWG 735


HSP 9 Score: 72.4 bits (176), Expect = 1.9e-11
Identity = 56/204 (27.45%), Postives = 97/204 (47.55%), Query Frame = 1

Query: 232  YSWGDNSHGQLGLGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLE 291
            Y+ G  + G+LG+G  +S + P ++E+        V  V  G  H   LS         E
Sbjct: 4009 YATGYGAGGRLGIGGTESVSTPTLLESIQHVFIKKV-AVNSGGKHCLALSS--------E 4068

Query: 292  SVCWTFGLGENGQLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWG 351
               +++G  E+G+LGHG       P+ ++ L + + ++ VA G  H++ V++ GDL++WG
Sbjct: 4069 GEVYSWGEAEDGKLGHGNRSPCDRPRVIESL-RGIEVVDVAAGGAHSACVTAAGDLYTWG 4128

Query: 352  MERGLGLCPDASFTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGA--AHTVIVAHDGY 411
              R            +++ D + P  +     E+ +    + +ACG+  A T+ +  D  
Sbjct: 4129 KGR------YGRLGHSDSEDQLKPKLV-----EALQGHRVIDIACGSGDAQTLCLTDDD- 4188

Query: 412  KLWSWGRGRSGVLGNGKAVDCFTP 434
             +WSWG G  G LG G +  C  P
Sbjct: 4189 TVWSWGDGDYGKLGRGGSDGCKVP 4190


HSP 10 Score: 48.1 bits (113), Expect = 3.8e-04
Identity = 29/78 (37.18%), Postives = 40/78 (51.28%), Query Frame = 1

Query: 7    RPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSG 66
            R   G    KV+AI+ G  H +  T DG VY+WG    G+LG G  +    P   +L + 
Sbjct: 4243 RQVQGLQGKKVIAIATGSLHCVCCTEDGEVYTWGDNDEGQLGDGTTNAIQRP---RLVAA 4302

Query: 67   EENLKVVAIAAGSYHSLA 85
             +  KV  +A GS H+LA
Sbjct: 4303 LQGKKVNRVACGSAHTLA 4317


HSP 11 Score: 38.5 bits (88), Expect = 3.0e-01
Identity = 40/146 (27.40%), Postives = 63/146 (43.15%), Query Frame = 1

Query: 297  FGLGEN--GQLGHGTTQNEFLPQPVKELP--QSVSLISVACGLFHTSVVSSGGDLWSWGM 356
            +G G N  GQLG        +P P + L   + V LI     LF    V++ G L++ G 
Sbjct: 3957 YGWGHNHRGQLGGIEGAKVKVPTPCEALATLRPVQLIGGEQTLF---AVTADGKLYATGY 4016

Query: 357  ERG--LGLCPDASFTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYK 416
              G  LG+    S +     ++I  +           F+  V V  G  H + ++ +G +
Sbjct: 4017 GAGGRLGIGGTESVSTPTLLESIQHV-----------FIKKVAVNSGGKHCLALSSEG-E 4076

Query: 417  LWSWGRGRSGVLGNGKAVDCFTPTVV 437
            ++SWG    G LG+G    C  P V+
Sbjct: 4077 VYSWGEAEDGKLGHGNRSPCDRPRVI 4087

BLAST of Cucsa.161260.1 vs. TrEMBL
Match: A0A0A0LPS2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G373550 PE=4 SV=1)

HSP 1 Score: 1188.7 bits (3074), Expect = 0.0e+00
Identity = 581/588 (98.81%), Postives = 581/588 (98.81%), Query Frame = 1

Query: 1   MEESGTRPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVR 60
           MEESGTRPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVR
Sbjct: 1   MEESGTRPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVR 60

Query: 61  VKLGSGEENLKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLE 120
           VKLGSGEENLKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLE
Sbjct: 61  VKLGSGEENLKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLE 120

Query: 121 LEPPESLTEESGSKSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGEN 180
           LEPPESLTEESGSKSKGSLK       ICDIKAGGQISFAIDDLGALWMWGNCPKPSGEN
Sbjct: 121 LEPPESLTEESGSKSKGSLK-------ICDIKAGGQISFAIDDLGALWMWGNCPKPSGEN 180

Query: 181 EVGLSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHG 240
           EVGLSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHG
Sbjct: 181 EVGLSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHG 240

Query: 241 QLGLGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLG 300
           QLGLGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLG
Sbjct: 241 QLGLGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLG 300

Query: 301 ENGQLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERGLGLCP 360
           ENGQLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERGLGLCP
Sbjct: 301 ENGQLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERGLGLCP 360

Query: 361 DASFTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSG 420
           DASFTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSG
Sbjct: 361 DASFTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSG 420

Query: 421 VLGNGKAVDCFTPTVVLWPPLNEDFDQEETETKCEEDKVEENKTEEDKVEENKIEEVTEE 480
           VLGNGKAVDCFTPTVVLWPPLNEDFDQEETETKCEEDKVEENKTEEDKVEENKIEEVTEE
Sbjct: 421 VLGNGKAVDCFTPTVVLWPPLNEDFDQEETETKCEEDKVEENKTEEDKVEENKIEEVTEE 480

Query: 481 NKKLLMAMEEIKLLLDKLSIMERYASFLHGSIFGKPFQEGDIPLSLRNSGSFDIAKEWES 540
           NKKLLMAMEEIKLLLDKLSIMERYASFLHGSIFGKPFQEGDIPLSLRNSGSFDIAKEWES
Sbjct: 481 NKKLLMAMEEIKLLLDKLSIMERYASFLHGSIFGKPFQEGDIPLSLRNSGSFDIAKEWES 540

Query: 541 MLESSDRSNLLRLETVYQNMLASIKDKILKKRVQELVKEFLQSATPPN 589
           MLESSDRSNLLRLETVYQNMLASIKDKILKKRVQELVKEFLQSATPPN
Sbjct: 541 MLESSDRSNLLRLETVYQNMLASIKDKILKKRVQELVKEFLQSATPPN 581

BLAST of Cucsa.161260.1 vs. TrEMBL
Match: A0A061GK39_THECC (Regulator of chromosome condensation family protein isoform 2 OS=Theobroma cacao GN=TCM_037200 PE=4 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 7.1e-236
Identity = 398/583 (68.27%), Postives = 470/583 (80.62%), Query Frame = 1

Query: 4   SGTRPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKL 63
           +GT P S  L  KVVA+SAGEAHTL L+GDG VYSWGRGMFGRLGTG+ESD  FPVRVK 
Sbjct: 5   AGTFPGSSNLFRKVVAVSAGEAHTLALSGDGCVYSWGRGMFGRLGTGSESDGHFPVRVKF 64

Query: 64  GSGEENLKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLELEP 123
            + E  LK VA+AAG+YHSLAL DDGSVW WGYNIYGQLG  G NS  P LL++FLEL  
Sbjct: 65  QNSE--LKFVAVAAGAYHSLALADDGSVWSWGYNIYGQLGVHGENSLAPQLLERFLELGS 124

Query: 124 PESLTEESGSKSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVG 183
           P+   +E    +K  LK       IC +KAGG  S AID+LGALWMWG CP+ +  ++ G
Sbjct: 125 PDQSKDELEINTKTPLK-------ICAVKAGGMTSLAIDNLGALWMWGLCPQENSSSDGG 184

Query: 184 LSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLG 243
           L++VSSF+P PVW+FHGHTVVKVACGNEH+VALVS GETY K +DLVCYSWG N HGQLG
Sbjct: 185 LTFVSSFSPIPVWDFHGHTVVKVACGNEHVVALVSAGETY-KGDDLVCYSWGGNGHGQLG 244

Query: 244 LGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENG 303
           LGD++SR  P+I+ETFN+  PW VYEVACGAFHTALL+H KRP +TLES+CWTFGLG+ G
Sbjct: 245 LGDRESRVHPEIVETFNQDIPWTVYEVACGAFHTALLTHRKRPSETLESMCWTFGLGDKG 304

Query: 304 QLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERGLGLCPDAS 363
           QLGHGTTQ+  +P+PVKELPQ V L+SV CGLFHTSVVSS GD+WSWGME+GLGLCPDAS
Sbjct: 305 QLGHGTTQSTLVPEPVKELPQPVYLVSVDCGLFHTSVVSSAGDVWSWGMEKGLGLCPDAS 364

Query: 364 FTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSGVLG 423
           FTGT+ GDA+SPL I   G   PKF DPVQ++CGAAHTV+VAHDGYKLWSWGRGRSGVLG
Sbjct: 365 FTGTDAGDALSPLQISGDGLHEPKFRDPVQISCGAAHTVVVAHDGYKLWSWGRGRSGVLG 424

Query: 424 NGKAVDCFTPTVVLWPPLNEDFDQEE-TETKCEEDKVEENKTEEDKVEENKIEEVTEENK 483
           NGK +DCFTP++VLWPPLNEDF QEE   T  E DK+ ++K           +  +E +K
Sbjct: 425 NGKTIDCFTPSMVLWPPLNEDFQQEELNSTVGEGDKIVDHKNS---------DAGSEMDK 484

Query: 484 KLLMAMEEIKLLLDKLSIMERYASFLHGSIFGKPFQEGDIPLSLRNSGSFDIAKEWESML 543
           KL +AMEE+KLL  KLSIMERYAS LHGSIFGKPF+E DIP+SL+NSG+FD+A+EWE+ML
Sbjct: 485 KLSLAMEEMKLLQSKLSIMERYASVLHGSIFGKPFEEQDIPISLQNSGTFDVAREWENML 544

Query: 544 ESSDRSNLLRLETVYQNMLASIKDKILKKRVQELVKEFLQSAT 586
           ESSDRS L+RLE  Y+NMLA +KDK++KKR+QE++KE+L S+T
Sbjct: 545 ESSDRSKLVRLELFYRNMLAGVKDKMMKKRIQEIIKEYLPSST 568

BLAST of Cucsa.161260.1 vs. TrEMBL
Match: A0A061GKT1_THECC (Regulator of chromosome condensation family protein isoform 1 OS=Theobroma cacao GN=TCM_037200 PE=4 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 3.9e-226
Identity = 390/595 (65.55%), Postives = 465/595 (78.15%), Query Frame = 1

Query: 4   SGTRPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKL 63
           +GT P S  L  KVVA+SAGEAHTL L+GDG VYSWGRGMFGRLGTG+ESD  FPVRVK 
Sbjct: 5   AGTFPGSSNLFRKVVAVSAGEAHTLALSGDGCVYSWGRGMFGRLGTGSESDGHFPVRVKF 64

Query: 64  GSGEENLKVVAIAAGSYHSLALTDDGSVWCWGYNIY------------GQLGFDGGNSAT 123
            + E  LK VA+AAG+YHSLAL DDGS      +++            GQLG  G NS  
Sbjct: 65  QNSE--LKFVAVAAGAYHSLALADDGSYLFSLLSVFMYFGISLDCHMDGQLGVHGENSLA 124

Query: 124 PCLLKQFLELEPPESLTEESGSKSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWG 183
           P LL++FLEL  P+   +E    +K  LK       IC +KAGG  S AID+LGALWMWG
Sbjct: 125 PQLLERFLELGSPDQSKDELEINTKTPLK-------ICAVKAGGMTSLAIDNLGALWMWG 184

Query: 184 NCPKPSGENEVGLSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVC 243
            CP+ +  ++ GL++VSSF+P PVW+FHGHTVVKVACGNEH+VALVS GETY K +DLVC
Sbjct: 185 LCPQENSSSDGGLTFVSSFSPIPVWDFHGHTVVKVACGNEHVVALVSAGETY-KGDDLVC 244

Query: 244 YSWGDNSHGQLGLGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLE 303
           YSWG N HGQLGLGD++SR  P+I+ETFN+  PW VYEVACGAFHTALL+H KRP +TLE
Sbjct: 245 YSWGGNGHGQLGLGDRESRVHPEIVETFNQDIPWTVYEVACGAFHTALLTHRKRPSETLE 304

Query: 304 SVCWTFGLGENGQLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWG 363
           S+CWTFGLG+ GQLGHGTTQ+  +P+PVKELPQ V L+SV CGLFHTSVVSS GD+WSWG
Sbjct: 305 SMCWTFGLGDKGQLGHGTTQSTLVPEPVKELPQPVYLVSVDCGLFHTSVVSSAGDVWSWG 364

Query: 364 MERGLGLCPDASFTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKL 423
           ME+GLGLCPDASFTGT+ GDA+SPL I   G   PKF DPVQ++CGAAHTV+VAHDGYKL
Sbjct: 365 MEKGLGLCPDASFTGTDAGDALSPLQISGDGLHEPKFRDPVQISCGAAHTVVVAHDGYKL 424

Query: 424 WSWGRGRSGVLGNGKAVDCFTPTVVLWPPLNEDFDQEE-TETKCEEDKVEENKTEEDKVE 483
           WSWGRGRSGVLGNGK +DCFTP++VLWPPLNEDF QEE   T  E DK+ ++K       
Sbjct: 425 WSWGRGRSGVLGNGKTIDCFTPSMVLWPPLNEDFQQEELNSTVGEGDKIVDHKNS----- 484

Query: 484 ENKIEEVTEENKKLLMAMEEIKLLLDKLSIMERYASFLHGSIFGKPFQEGDIPLSLRNSG 543
               +  +E +KKL +AMEE+KLL  KLSIMERYAS LHGSIFGKPF+E DIP+SL+NSG
Sbjct: 485 ----DAGSEMDKKLSLAMEEMKLLQSKLSIMERYASVLHGSIFGKPFEEQDIPISLQNSG 544

Query: 544 SFDIAKEWESMLESSDRSNLLRLETVYQNMLASIKDKILKKRVQELVKEFLQSAT 586
           +FD+A+EWE+MLESSDRS L+RLE  Y+NMLA +KDK++KKR+QE++KE+L S+T
Sbjct: 545 TFDVAREWENMLESSDRSKLVRLELFYRNMLAGVKDKMMKKRIQEIIKEYLPSST 580

BLAST of Cucsa.161260.1 vs. TrEMBL
Match: F6H3D2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0008g03320 PE=4 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 1.2e-214
Identity = 382/618 (61.81%), Postives = 455/618 (73.62%), Query Frame = 1

Query: 1   MEESGTRPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVR 60
           ME++GT P SG L GK+V+++AGEAH+LVLTGDGRVYSWGRG FGRLGTG+E+DELFPV 
Sbjct: 1   MEDAGTVPESGNLRGKIVSVAAGEAHSLVLTGDGRVYSWGRGTFGRLGTGSEADELFPVP 60

Query: 61  VKLGSGEEN----LKVVAIAAGSYHSLALTDDGSVWCWGYN---IY-------------- 120
           +K  S E++    +K V IAAG+YHS+AL DDG+VWCWGYN   +Y              
Sbjct: 61  IKFDSAEKSDSKGVKFVGIAAGAYHSVALADDGTVWCWGYNSCILYHGDTWNLFCTFARS 120

Query: 121 ----------------GQLGFDGGNSATPCLLKQFLELEPPESLTEESGSKSKGSLKFPL 180
                           GQLGF   N   P LL+ FL L    SLT+ES +K+K  LK   
Sbjct: 121 VCFFVFLRFILDCYMDGQLGFSDDNCLVPHLLELFLGLGSLGSLTDESDTKNKTPLK--- 180

Query: 181 LLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVGLSYVSSFTPSPVWEFHGHTV 240
               +  +KAGG +S AID+LG LWMWGN P+ S  ++   S VSSFTP PVW+FHGHTV
Sbjct: 181 ----VNAVKAGGMMSLAIDNLGGLWMWGNVPE-SSPDKGEFSLVSSFTPIPVWDFHGHTV 240

Query: 241 VKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACPQIIETFNEAS 300
           VKVACGNEH+VALVS GETY K  DL+CYSWG+N HGQLGLGD ++R  P++++TFN  S
Sbjct: 241 VKVACGNEHVVALVSHGETY-KGGDLICYSWGNNGHGQLGLGDTENRLRPEVVKTFNLES 300

Query: 301 PWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHGTTQNEFLPQPVKELP 360
            W VYEVACGAFHTALL+H KRP DTLES+CWTFGLG+NGQLGHGTT+   LP+PVK+LP
Sbjct: 301 LWEVYEVACGAFHTALLTHKKRPSDTLESMCWTFGLGDNGQLGHGTTKGISLPEPVKDLP 360

Query: 361 QSVSLISVACGLFHTSVVSSGGDLWSWGMERGLGLCPDASFTGTNTGDAISPLSIFCSGT 420
           +   L+SV CGLFHTSVVSS G++WSWGME+GLGLCPDASFTGT+ GDA SPL       
Sbjct: 361 EHRFLVSVDCGLFHTSVVSSAGEVWSWGMEKGLGLCPDASFTGTDPGDAPSPL------L 420

Query: 421 ESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSGVLGNGKAVDCFTPTVVLWPPLNE 480
              KF DPVQVACGAAHTV+VA DGYKLWSWGRGRSGVLGNGK VDCF P+ VLWPPL E
Sbjct: 421 YGSKFRDPVQVACGAAHTVLVADDGYKLWSWGRGRSGVLGNGKTVDCFAPSTVLWPPLTE 480

Query: 481 DFDQEETETKCEEDKVEENKTEEDKVEENKIEEVTEENKKLLMAMEEIKLLLDKLSIMER 540
           DF + E+ T           + E K+E    EEVTE  K+L +A EE+KLL  KLS MER
Sbjct: 481 DFKELESGT----------TSGEKKIENKDPEEVTEMEKRLSLATEEMKLLHSKLSTMER 540

Query: 541 YASFLHGSIFGKPFQEGDIPLSLRNSGSFDIAKEWESMLESSDRSNLLRLETVYQNMLAS 582
           YAS LHGSIFGKPF+E DIP+SL+NSG FDIAKEWE+MLES+DR  L+ LE  Y+NMLA 
Sbjct: 541 YASILHGSIFGKPFEEQDIPISLQNSGFFDIAKEWENMLESADRGKLVMLEKFYRNMLAG 593

BLAST of Cucsa.161260.1 vs. TrEMBL
Match: B9RRY8_RICCO (Protein pim1, putative OS=Ricinus communis GN=RCOM_0799870 PE=4 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 4.5e-214
Identity = 379/591 (64.13%), Postives = 445/591 (75.30%), Query Frame = 1

Query: 1   MEESGT-RPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPV 60
           ME+ G     SG LS KVV+++AGEAHTL LTGDG VYSWGRG FGRLGTG E DEL+PV
Sbjct: 1   MEDIGAVTGGSGNLSCKVVSVAAGEAHTLALTGDGCVYSWGRGTFGRLGTGLEKDELYPV 60

Query: 61  RVKLGSGEE----NLKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLL 120
           +VK    EE     LK V IAAG+YHS+AL DDGSVWCWGYNI      D  N+A P ++
Sbjct: 61  KVKFDMCEEFEERKLKFVGIAAGAYHSVALADDGSVWCWGYNI---CILDPENAAVPRMM 120

Query: 121 KQFLELEPPESLTEESGSKSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPK 180
           +QF EL  P S T ES +  +  LK       +C +KAGG +S AID+LGALWMWGNCP+
Sbjct: 121 EQFHELIYPGSATNESETNREVPLK-------VCSVKAGGMMSLAIDNLGALWMWGNCPQ 180

Query: 181 PSGENEVGLSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWG 240
            + E+  G S VSSFTP PVW+F+GHTVVKVACGNEH+VALVS GETY K EDL+CYSWG
Sbjct: 181 QNSES--GFSLVSSFTPIPVWDFYGHTVVKVACGNEHVVALVSEGETY-KGEDLLCYSWG 240

Query: 241 DNSHGQLGLGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCW 300
           +N+HGQLGLGD +SR CP+I+ETFN+ SPWAVYEV+CGAFHTALL+  KRP D L+S CW
Sbjct: 241 NNNHGQLGLGDTESRVCPEIVETFNQDSPWAVYEVSCGAFHTALLTCKKRPNDKLQSTCW 300

Query: 301 TFGLGENGQLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERG 360
           TFGLGENGQLG GTTQN   P+PVKELP+   LISV CGLFHTSVVSS GD+WSWGM  G
Sbjct: 301 TFGLGENGQLGLGTTQNALKPEPVKELPEYAYLISVDCGLFHTSVVSSAGDVWSWGMMNG 360

Query: 361 LGLCPDASFTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWG 420
           LGLCPDA FTGT+ GDAISPL I  +GT   KF DPVQ+ CGAAHTV+V + GYKLWSWG
Sbjct: 361 LGLCPDARFTGTDAGDAISPLLI--TGT---KFNDPVQITCGAAHTVLVTNGGYKLWSWG 420

Query: 421 RGRSGVLGNGKAVDCFTPTVVLWPPLNEDFDQEETETKCEEDKVEENKTEEDKVEENKIE 480
           RGRSG LGNGK +D   P V+LWPPL E    +E+           N  EED + +   E
Sbjct: 421 RGRSGALGNGKEIDVLVPGVMLWPPLTEVVKNQES----------NNSVEEDNLVKKGSE 480

Query: 481 EVTEENKKLLMAMEEIKLLLDKLSIMERYASFLHGSIFGKPFQEGDIPLSLRNSGSFDIA 540
           EVTE   KL +AMEE+KLL  KL+IMERYA  LHGSIFGKPF E D+P+SL+NS +FDIA
Sbjct: 481 EVTEMENKLSLAMEEMKLLQSKLTIMERYAGILHGSIFGKPFAEDDLPISLQNSDTFDIA 540

Query: 541 KEWESMLESSDRSNLLRLETVYQNMLASIKDKILKKRVQELVKEFLQSATP 587
           K WE MLES+DRS L+RLE  Y+NMLA +KDK +K+R+QE+++E+L S TP
Sbjct: 541 KAWEEMLESADRSKLIRLEIFYRNMLAGVKDKKMKRRIQEIIQEYLPSVTP 563

BLAST of Cucsa.161260.1 vs. TAIR10
Match: AT5G11580.1 (AT5G11580.1 Regulator of chromosome condensation (RCC1) family protein)

HSP 1 Score: 627.5 bits (1617), Expect = 8.4e-180
Identity = 320/590 (54.24%), Postives = 404/590 (68.47%), Query Frame = 1

Query: 2   EESGTRPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRV 61
           ++S T   S  LS K+++++AGEAHT+ LTGDG VYSWGRGMFGRLGTG ESDEL PV V
Sbjct: 3   DKSPTLLISEDLSRKIISLAAGEAHTIALTGDGCVYSWGRGMFGRLGTGKESDELVPVLV 62

Query: 62  KLGSGEEN--LKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFL 121
           +  +  E   +++V +AAG+YHSLA++DDGSVWCWGYNIYGQLGFDG NS  PCL+K   
Sbjct: 63  EFPNQAEGDRIRIVGVAAGAYHSLAVSDDGSVWCWGYNIYGQLGFDGENSLAPCLIKNLF 122

Query: 122 ELEPPESLTEESGSKSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGE 181
           E E   S   +SG +    LK       +C +KAG  +S AID++G LWMWGN P    E
Sbjct: 123 ERETSSSSLNDSGREVGSDLK-------VCTVKAGSMMSLAIDNVGGLWMWGNVPPQDSE 182

Query: 182 NEVGLSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETY--NKAEDLVCYSWGDN 241
            +  LS+ S   P P+ +FHG TV+KVACG+EH+VALV  G+ +  N  +  V YSWG+N
Sbjct: 183 PDPRLSFTSIPIPFPILDFHGRTVLKVACGDEHVVALVGPGDIHKDNSYDVSVLYSWGNN 242

Query: 242 SHGQLGLGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTF 301
            HGQLGLGD +SRA PQ +ETFN+ S   VY++ACGA HTALL++ K       S+CWTF
Sbjct: 243 HHGQLGLGDGESRARPQTVETFNQKSGLTVYDIACGAHHTALLTYRKETPKG-PSICWTF 302

Query: 302 GLGENGQLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERGLG 361
           G GENGQLGH + ++  LP+PV +LP+   L+SV CGLFHTSVVSS G +WSWGMERGLG
Sbjct: 303 GFGENGQLGHRSNKSSSLPEPVSDLPEHAYLVSVDCGLFHTSVVSSEGYVWSWGMERGLG 362

Query: 362 LCPDASFTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRG 421
           LCPD +FT    GD   P  I  SG  S +F DPVQV+CGAAHTV+V   GYKLWSWGRG
Sbjct: 363 LCPDVNFTEVEAGDDSVPRKI--SGGSS-RFRDPVQVSCGAAHTVLVVDGGYKLWSWGRG 422

Query: 422 RSGVLGNGKAVDCFTPTVVLWPPLNEDFDQEETETKCEEDKVEENKTEEDKVEENKIEEV 481
           R+GVLG G   DC+ PT+V WP                      N+ + +K      EEV
Sbjct: 423 RNGVLGTGNVSDCYVPTLVFWP----------------------NELKPEK------EEV 482

Query: 482 TEENKKLLMAMEEIKLLLDKLSIMERYASFLHGSIFGKPF-QEGDIPLSLRNSGSFDIAK 541
            ++ K    + EEIK L  KL +MERYAS LHGSIFGKPF +E DIP SLR SG FD+ K
Sbjct: 483 PDDGKS--ASTEEIKRLESKLMVMERYASILHGSIFGKPFNEEEDIPYSLRVSGYFDMGK 542

Query: 542 EWESMLESSDRSNLLRLETVYQNMLASIKDKILKKRVQELVKEFLQSATP 587
           EW  MLES+D+S L+RL+  Y++M+  +KDK+L++R+QE++K+ LQS+ P
Sbjct: 543 EWGEMLESADKSQLMRLQAFYEDMIGRVKDKVLQRRIQEIMKDCLQSSAP 551

BLAST of Cucsa.161260.1 vs. TAIR10
Match: AT5G63860.1 (AT5G63860.1 Regulator of chromosome condensation (RCC1) family protein)

HSP 1 Score: 152.1 bits (383), Expect = 1.0e-36
Identity = 123/427 (28.81%), Postives = 189/427 (44.26%), Query Frame = 1

Query: 16  KVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSGEENLKVVAI 75
           KV+ ISAG +H++ L     V SWGRG  G+LG G   D   P ++    G +   +V++
Sbjct: 16  KVLIISAGASHSVALLSGDIVCSWGRGEDGQLGHGDAEDRPSPTQLSALDGHQ---IVSV 75

Query: 76  AAGSYHSLALTDDG-SVWCWGYNIYGQLGFDGGNSATPCLLKQFLELEPPESLTEESGSK 135
             G+ H++A +  G  V+ WG+  +G+LG   GNS+         +L  P  +    G +
Sbjct: 76  TCGADHTVAYSQSGMEVYSWGWGDFGRLGH--GNSS---------DLFTPLPIKALHGIR 135

Query: 136 SKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVGLSYV-SSFTPS 195
            K              I  G     A+   G +  WG     +   ++GL     S  P 
Sbjct: 136 IK-------------QIACGDSHCLAVTMEGEVQSWGR----NQNGQLGLGDTEDSLVPQ 195

Query: 196 PVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACP 255
            +  F G  +  VA G EH  A+   G+          Y WG   +G LGLGD+  R  P
Sbjct: 196 KIQAFEGIRIKMVAAGAEHTAAVTEDGD---------LYGWGWGRYGNLGLGDRTDRLVP 255

Query: 256 QIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHGTTQNE 315
              E         +  VACG  HT  +S+S           +T+G  + GQLGHG  ++ 
Sbjct: 256 ---ERVTSTGGEKMSMVACGWRHTISVSYS--------GALYTYGWSKYGQLGHGDLEDH 315

Query: 316 FLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMER----GLGLCPDASFTGTNT 375
            +P  ++ L  S  +  ++ G  HT  ++S G L+ WG  +    G+G          N 
Sbjct: 316 LIPHKLEALSNSF-ISQISGGWRHTMALTSDGKLYGWGWNKFGQVGVG----------NN 374

Query: 376 GDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSGVLGNGKAVD 435
            D  SP+ +       P     VQV+CG  HT+ V  +   +++WGRG +G LG G++VD
Sbjct: 376 LDQCSPVQV-----RFPDDQKVVQVSCGWRHTLAVT-ERNNVFAWGRGTNGQLGIGESVD 374

Query: 436 CFTPTVV 437
              P ++
Sbjct: 436 RNFPKII 374

BLAST of Cucsa.161260.1 vs. TAIR10
Match: AT5G08710.1 (AT5G08710.1 Regulator of chromosome condensation (RCC1) family protein)

HSP 1 Score: 126.3 bits (316), Expect = 6.1e-29
Identity = 123/419 (29.36%), Postives = 174/419 (41.53%), Query Frame = 1

Query: 39  WGRGMFGRLGTGAESDELFPVRVKLGSGEENLKVVAIAAGSYHSLALTDDGSVWCWGYNI 98
           WG G +GRLG G    +  P    + S   +  + A+A G  H+L LT+   V+  G N 
Sbjct: 54  WGSGDYGRLGLGNLDSQWTPA---VCSALSDHSITAVACGGAHTLFLTETRRVFATGLND 113

Query: 99  YGQLGFDGGNSATPCLLKQFLELEPPESLTEESGSKSKGSLKFPLLLYQICDIKAGGQIS 158
            GQLG     S           ++P     E SG           L   I  I AG   S
Sbjct: 114 CGQLGVSDVKSHA---------MDP----LEVSG-----------LDKDILHISAGYYHS 173

Query: 159 FAIDDLGALWMWGNCPKPSGENEVGLSYVSSF-TPSPVWEFHGHTVVKVACGNEHIVALV 218
            AI   G L+MWG     SG+  +G         P+ V   HG T+  VA G+EH VA+ 
Sbjct: 174 AAITVDGELYMWGK--NSSGQLGLGKKAARVVRVPTKVEALHGITIQSVALGSEHSVAVT 233

Query: 219 SVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACPQIIETFNEASPWAVYE-------- 278
             GE           SWG    G+LG G + S     I+ + +E +P  + E        
Sbjct: 234 DGGEVL---------SWGGGGSGRLGHGHQSSLF--GILRSNSEFTPRLIKELEGIKVTN 293

Query: 279 VACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHGTTQNEFLPQPVKELPQSVSLI 338
           VA G  H+A        C       + FG     ++G G  +N   P  + E+P +    
Sbjct: 294 VAAGLLHSA--------CTDENGSAFMFGEKSINKMGFGGVRNATTPSIISEVPYAEE-- 353

Query: 339 SVACGLFHTSVVSSGGDLWSWGMERGLGLCPDASFTGTNTGDAISPLSIFCSGTESPKFL 398
            VACG +HT VV+ GG+L++WG      L  D+++       + SP+ +     E P FL
Sbjct: 354 -VACGGYHTCVVTRGGELYTWGSNENGCLGTDSTYV------SHSPVRV-----EGP-FL 409

Query: 399 DPV--QVACGAAHTVIVAHDGYKLWSW----------GRGRSGVLGNGKAVDCFTPTVV 437
           +    QV+CG  HT  ++ +    W W          G    G LG+G  VD   P +V
Sbjct: 414 ESTVSQVSCGWKHTAAISDNNVFTWGWGGSHGTFSVDGHSSGGQLGHGSDVDYARPAMV 409

BLAST of Cucsa.161260.1 vs. TAIR10
Match: AT5G16040.1 (AT5G16040.1 Regulator of chromosome condensation (RCC1) family protein)

HSP 1 Score: 122.5 bits (306), Expect = 8.8e-28
Identity = 116/405 (28.64%), Postives = 170/405 (41.98%), Query Frame = 1

Query: 36  VYSWGRGMFGRLGTGAESD-ELFPVRVKLGSGEENLKVVAIAAGSYHSLALTDDGSVWCW 95
           V +WG G  G+LG G + + EL  V   L    E   V ++  GS +SLA+ DDG ++ W
Sbjct: 7   VIAWGSGEDGQLGLGTDEEKELASVVDAL----EPFNVRSVVGGSRNSLAICDDGKLFTW 66

Query: 96  GYNIYGQLGFDGGNSATPCLLKQFLELEPPESLTEESGSKSKGSLKFPLLLYQICDIKAG 155
           G+N  G LG                   PPE+ TE + S  K      L   +I     G
Sbjct: 67  GWNQRGTLG------------------HPPETKTESTPSLVKS-----LANVKIVQAAIG 126

Query: 156 GQISFAIDDLGALWMWGN-----CPKPSGENEVGLSYVSSFTPSPVWEFHGHTVVKVACG 215
           G    A+DD G  + WG      C +   ++E G   V      P       TV +VA G
Sbjct: 127 GWHCLAVDDQGRAYAWGGNEYGQCGEEPSKDETGRP-VRRDIVIPKRCAQQLTVRQVAAG 186

Query: 216 NEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACPQIIETFNEASPWAVYE 275
             H V L   G  +         +WG         GD    + P  ++         V  
Sbjct: 187 GTHSVVLTREGYVW---------TWGQ----PWPPGDIKQISVPVRVQGLEN-----VRL 246

Query: 276 VACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHGTTQNEFLPQPVKELPQSVSLI 335
           +A GAFH   L          +   W +G  E GQLG G TQ    P PV+ L   ++L+
Sbjct: 247 IAVGAFHNLALKE--------DGTLWAWGNNEYGQLGTGDTQPRSYPIPVQGL-DDLTLV 306

Query: 336 SVACGLFHTSVVSSGGDLWSWGM-ERGLGLCPDASFTGTNTGDAISPLSIFCSGTESPKF 395
            +A G +H++ +++ G+++ WG  E G        F   +    + P  +     E    
Sbjct: 307 DIAAGGWHSTALTNEGEVYGWGRGEHGR-----LGFGDNDKSSKMLPQKVNLLAGE---- 345

Query: 396 LDPVQVACGAAHTVIVAHDGYKLWSWGRGRSGVLGNGKAVDCFTP 434
            D +QV+CG  H+V +  DG +++S+GRG  G LG G+ V    P
Sbjct: 367 -DIIQVSCGGTHSVALTRDG-RIFSFGRGDHGRLGYGRKVTTGQP 345


HSP 2 Score: 95.1 bits (235), Expect = 1.5e-19
Identity = 81/274 (29.56%), Postives = 121/274 (44.16%), Query Frame = 1

Query: 20  ISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSGEENLKVVAIAAGS 79
           ++AG  H++VLT +G V++WG+        G       PVRV+   G EN++++A+  G+
Sbjct: 155 VAAGGTHSVVLTREGYVWTWGQPW----PPGDIKQISVPVRVQ---GLENVRLIAV--GA 214

Query: 80  YHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLELEPPESLTEESGSKSKGSL 139
           +H+LAL +DG++W WG N YGQLG       +  +  Q L     + LT           
Sbjct: 215 FHNLALKEDGTLWAWGNNEYGQLGTGDTQPRSYPIPVQGL-----DDLT----------- 274

Query: 140 KFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPS---GENEVGLSYVSSFTPSPVW 199
                   + DI AGG  S A+ + G ++ WG         G+N+      S   P  V 
Sbjct: 275 --------LVDIAAGGWHSTALTNEGEVYGWGRGEHGRLGFGDNDKS----SKMLPQKVN 334

Query: 200 EFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACPQII 259
              G  +++V+CG  H VAL   G           +S+G   HG+LG G K +   P  +
Sbjct: 335 LLAGEDIIQVSCGGTHSVALTRDGR---------IFSFGRGDHGRLGYGRKVTTGQPLEL 382

Query: 260 E--------TFN-----EASPWAVYEVACGAFHT 278
                     FN     +   W    VACG  HT
Sbjct: 395 PIHIPPPEGRFNHTDEEDDGKWIAKHVACGGRHT 382

BLAST of Cucsa.161260.1 vs. TAIR10
Match: AT1G69710.1 (AT1G69710.1 Regulator of chromosome condensation (RCC1) family with FYVE zinc finger domain)

HSP 1 Score: 119.0 bits (297), Expect = 9.8e-27
Identity = 98/340 (28.82%), Postives = 157/340 (46.18%), Query Frame = 1

Query: 20  ISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSGEENLKVVAIAAGS 79
           I+ G+ H +++T  G ++SWG G  G+LG G E+D   P   K  S    L   ++A G 
Sbjct: 305 IACGKCHAVLVTKQGEIFSWGEGKGGKLGHGLETDAQKP---KFISSVRGLGFKSLACGD 364

Query: 80  YHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLELEPPESLTEESGSKSKGSL 139
           +H+ A+T  G ++ WG   +       GN ++ C +        P+ +T        G L
Sbjct: 365 FHTCAITQSGDLYSWGDGTHNVDLLGHGNESS-CWI--------PKRVT--------GDL 424

Query: 140 KFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPS---GENEVGLSYVSSFTPSPVW 199
           +    LY + D+  G   +  +   G L+ +G+    +   G+        S+  P  V 
Sbjct: 425 QG---LY-VSDVACGPWHTAVVASSGQLFTFGDGTFGALGHGDRR------STSVPREVE 484

Query: 200 EFHGHTVVKVACGNEHIVALVSVGETYNKAE----DLVCYSWGDNSHGQLGLGDKDSRAC 259
              G  V KVACG  H  A+V V    ++AE        ++WGD   GQLG GD D++  
Sbjct: 485 SLIGLIVTKVACGVWHTAAVVEVTNEASEAEVDSSRGQVFTWGDGEKGQLGHGDNDTKLL 544

Query: 260 PQ-IIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHGTTQ 319
           P+ +I   NE     + +VACG        HS     T     +T G    GQLG+ T +
Sbjct: 545 PECVISLTNE----NICQVACG--------HSLTVSRTSRGHVYTMGSTAYGQLGNPTAK 601

Query: 320 NEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWG 352
             F P+ V+      S+  +ACG +H +V++S  ++++WG
Sbjct: 605 GNF-PERVEGDIVEASVEEIACGSYHVAVLTSKSEIYTWG 601


HSP 2 Score: 100.5 bits (249), Expect = 3.6e-21
Identity = 110/420 (26.19%), Postives = 172/420 (40.95%), Query Frame = 1

Query: 34  GRVYSWGRGMFGRL--GTG-----AESDELFPVRVKLGSGEENLKVVAIAAGSYHSLALT 93
           G V+ WG  +   +  GTG        D L P   K       L    IA G  H++ +T
Sbjct: 260 GDVFVWGESISDGVLSGTGNSLNSTTEDALLP---KALESTIVLDAQNIACGKCHAVLVT 319

Query: 94  DDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLELEPPESLTEESGSKSKGSLKFPLLLY 153
             G ++ WG    G+LG      A           + P+ ++   G   K          
Sbjct: 320 KQGEIFSWGEGKGGKLGHGLETDA-----------QKPKFISSVRGLGFKS--------- 379

Query: 154 QICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVGL----SYVSSFTPSPVW-EFHGH 213
               +  G   + AI   G L+ WG+     G + V L    +  S + P  V  +  G 
Sbjct: 380 ----LACGDFHTCAITQSGDLYSWGD-----GTHNVDLLGHGNESSCWIPKRVTGDLQGL 439

Query: 214 TVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACPQIIETFNE 273
            V  VACG  H   + S G+ +         ++GD + G LG GD+ S + P+ +E+   
Sbjct: 440 YVSDVACGPWHTAVVASSGQLF---------TFGDGTFGALGHGDRRSTSVPREVESLIG 499

Query: 274 ASPWAVYEVACGAFHTALLSH-----SKRPCDTLESVCWTFGLGENGQLGHGTTQNEFLP 333
                V +VACG +HTA +       S+   D+     +T+G GE GQLGHG    + LP
Sbjct: 500 L---IVTKVACGVWHTAAVVEVTNEASEAEVDSSRGQVFTWGDGEKGQLGHGDNDTKLLP 559

Query: 334 QPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERGLGLCPDASFTGTNTGDAISPL 393
           + V  L    ++  VACG   T   +S G +++ G      L       G  T     P 
Sbjct: 560 ECVISLTNE-NICQVACGHSLTVSRTSRGHVYTMGSTAYGQL-------GNPTAKGNFPE 619

Query: 394 SIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSGVLGNGKAVDCFTPTVV 437
            +     E+       ++ACG+ H  ++     ++++WG+G +G LG+G   +   P VV
Sbjct: 620 RVEGDIVEA----SVEEIACGSYHVAVLTSKS-EIYTWGKGLNGQLGHGNVENKREPAVV 622


HSP 3 Score: 88.2 bits (217), Expect = 1.8e-17
Identity = 86/334 (25.75%), Postives = 132/334 (39.52%), Query Frame = 1

Query: 108 NSATPCLLKQFLELEPPESLTEESGSKSKGSLKFPLLLYQICDIKAGGQISFAIDDLGAL 167
           N+ T      F    P  +  E S  +   +  F L L       + G      D LG +
Sbjct: 203 NNQTEASGNLFCPFSPTPANVENSNLRFSTNDPFRLSLSSAVSTSSHGSYHEDFDALGDV 262

Query: 168 WMWGNCPKPSGENEVGLSYVSSFTPSPVWEFHGHTVV----KVACGNEHIVALVSVGETY 227
           ++WG        +  G S  S+   + + +    T+V     +ACG  H V +   GE +
Sbjct: 263 FVWGESISDGVLSGTGNSLNSTTEDALLPKALESTIVLDAQNIACGKCHAVLVTKQGEIF 322

Query: 228 NKAEDLVCYSWGDNSHGQLGLGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHS 287
                    SWG+   G+LG G +     P+ I +           +ACG FHT  ++ S
Sbjct: 323 ---------SWGEGKGGKLGHGLETDAQKPKFISSVRGLG---FKSLACGDFHTCAITQS 382

Query: 288 KRPCDTLESVCWTFGLGENGQLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSS 347
                  +   W  G      LGHG   + ++P+ V    Q + +  VACG +HT+VV+S
Sbjct: 383 G------DLYSWGDGTHNVDLLGHGNESSCWIPKRVTGDLQGLYVSDVACGPWHTAVVAS 442

Query: 348 GGDLWSWGMERGLGLCPDASFTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVI 407
            G L+++G         D +F     GD  S  +      ES   L   +VACG  HT  
Sbjct: 443 SGQLFTFG---------DGTFGALGHGDRRS--TSVPREVESLIGLIVTKVACGVWHTAA 502

Query: 408 VAH------------DGYKLWSWGRGRSGVLGNG 426
           V                 ++++WG G  G LG+G
Sbjct: 503 VVEVTNEASEAEVDSSRGQVFTWGDGEKGQLGHG 507

BLAST of Cucsa.161260.1 vs. NCBI nr
Match: gi|778672037|ref|XP_004148391.2| (PREDICTED: ultraviolet-B receptor UVR8 [Cucumis sativus])

HSP 1 Score: 1188.7 bits (3074), Expect = 0.0e+00
Identity = 581/588 (98.81%), Postives = 581/588 (98.81%), Query Frame = 1

Query: 1   MEESGTRPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVR 60
           MEESGTRPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVR
Sbjct: 1   MEESGTRPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVR 60

Query: 61  VKLGSGEENLKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLE 120
           VKLGSGEENLKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLE
Sbjct: 61  VKLGSGEENLKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLE 120

Query: 121 LEPPESLTEESGSKSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGEN 180
           LEPPESLTEESGSKSKGSLK       ICDIKAGGQISFAIDDLGALWMWGNCPKPSGEN
Sbjct: 121 LEPPESLTEESGSKSKGSLK-------ICDIKAGGQISFAIDDLGALWMWGNCPKPSGEN 180

Query: 181 EVGLSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHG 240
           EVGLSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHG
Sbjct: 181 EVGLSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHG 240

Query: 241 QLGLGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLG 300
           QLGLGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLG
Sbjct: 241 QLGLGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLG 300

Query: 301 ENGQLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERGLGLCP 360
           ENGQLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERGLGLCP
Sbjct: 301 ENGQLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERGLGLCP 360

Query: 361 DASFTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSG 420
           DASFTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSG
Sbjct: 361 DASFTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSG 420

Query: 421 VLGNGKAVDCFTPTVVLWPPLNEDFDQEETETKCEEDKVEENKTEEDKVEENKIEEVTEE 480
           VLGNGKAVDCFTPTVVLWPPLNEDFDQEETETKCEEDKVEENKTEEDKVEENKIEEVTEE
Sbjct: 421 VLGNGKAVDCFTPTVVLWPPLNEDFDQEETETKCEEDKVEENKTEEDKVEENKIEEVTEE 480

Query: 481 NKKLLMAMEEIKLLLDKLSIMERYASFLHGSIFGKPFQEGDIPLSLRNSGSFDIAKEWES 540
           NKKLLMAMEEIKLLLDKLSIMERYASFLHGSIFGKPFQEGDIPLSLRNSGSFDIAKEWES
Sbjct: 481 NKKLLMAMEEIKLLLDKLSIMERYASFLHGSIFGKPFQEGDIPLSLRNSGSFDIAKEWES 540

Query: 541 MLESSDRSNLLRLETVYQNMLASIKDKILKKRVQELVKEFLQSATPPN 589
           MLESSDRSNLLRLETVYQNMLASIKDKILKKRVQELVKEFLQSATPPN
Sbjct: 541 MLESSDRSNLLRLETVYQNMLASIKDKILKKRVQELVKEFLQSATPPN 581

BLAST of Cucsa.161260.1 vs. NCBI nr
Match: gi|659088493|ref|XP_008445010.1| (PREDICTED: probable E3 ubiquitin-protein ligase HERC4 isoform X2 [Cucumis melo])

HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 546/588 (92.86%), Postives = 560/588 (95.24%), Query Frame = 1

Query: 1   MEESGTRPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVR 60
           M+ESGTRPSS KLSGKVV I+AGEAHT+VLTGDGRVYSWGRGMFGRLGTGAESDELFPVR
Sbjct: 1   MDESGTRPSSSKLSGKVVEIAAGEAHTIVLTGDGRVYSWGRGMFGRLGTGAESDELFPVR 60

Query: 61  VKLGSGEENLKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLE 120
           VKLGS EENLKVVAIAAGSYHSLAL DDGSVWCWGYN+YGQLGFDGGNSATPCLLKQFLE
Sbjct: 61  VKLGSEEENLKVVAIAAGSYHSLALADDGSVWCWGYNLYGQLGFDGGNSATPCLLKQFLE 120

Query: 121 LEPPESLTEESGSKSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGEN 180
           LEPPESLTEESGSKSKGSLK       ICDIKAGGQISFAIDDLGALWMWGNCPKP+GEN
Sbjct: 121 LEPPESLTEESGSKSKGSLK-------ICDIKAGGQISFAIDDLGALWMWGNCPKPNGEN 180

Query: 181 EVGLSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHG 240
           EV LSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHG
Sbjct: 181 EVSLSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHG 240

Query: 241 QLGLGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLG 300
           QLGLGD+DSRA PQIIETFNEASPWAVYE+ACGAFHTAL+S SKRPCDTLESVCWTFGLG
Sbjct: 241 QLGLGDRDSRARPQIIETFNEASPWAVYEIACGAFHTALVSRSKRPCDTLESVCWTFGLG 300

Query: 301 ENGQLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERGLGLCP 360
           ENGQLGHGTTQNEFLPQPVKELP+SVSLISV CGLFHTSVVSSGGDLWSWGMERGLGLCP
Sbjct: 301 ENGQLGHGTTQNEFLPQPVKELPKSVSLISVDCGLFHTSVVSSGGDLWSWGMERGLGLCP 360

Query: 361 DASFTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSG 420
           DASFTGTNTGDAISPLSIFCSG+ESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSG
Sbjct: 361 DASFTGTNTGDAISPLSIFCSGSESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSG 420

Query: 421 VLGNGKAVDCFTPTVVLWPPLNEDFDQEETETKCEEDKVEENKTEEDKVEENKIEEVTEE 480
           VLGNGKAVDCFTPTVVLWPPL+EDFDQEETETK EEDKVEENKT          EEVTEE
Sbjct: 421 VLGNGKAVDCFTPTVVLWPPLDEDFDQEETETKSEEDKVEENKT----------EEVTEE 480

Query: 481 NKKLLMAMEEIKLLLDKLSIMERYASFLHGSIFGKPFQEGDIPLSLRNSGSFDIAKEWES 540
           NKKLLMAMEEIKLLL+KLSIMERYASFLHGSIFGKPFQEGDIPLSLRNSGSFDIAKEWES
Sbjct: 481 NKKLLMAMEEIKLLLEKLSIMERYASFLHGSIFGKPFQEGDIPLSLRNSGSFDIAKEWES 540

Query: 541 MLESSDRSNLLRLETVYQNMLASIKDKILKKRVQELVKEFLQSATPPN 589
           MLESSDRSNLLRLE VYQNMLASIKDKI+KKRVQELVKEFLQS+T PN
Sbjct: 541 MLESSDRSNLLRLEVVYQNMLASIKDKIMKKRVQELVKEFLQSSTSPN 571

BLAST of Cucsa.161260.1 vs. NCBI nr
Match: gi|659088491|ref|XP_008445009.1| (PREDICTED: regulator of chromosome condensation isoform X1 [Cucumis melo])

HSP 1 Score: 1064.7 bits (2752), Expect = 5.9e-308
Identity = 520/557 (93.36%), Postives = 531/557 (95.33%), Query Frame = 1

Query: 32  GDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSGEENLKVVAIAAGSYHSLALTDDGSV 91
           GDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGS EENLKVVAIAAGSYHSLAL DDGSV
Sbjct: 37  GDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSEEENLKVVAIAAGSYHSLALADDGSV 96

Query: 92  WCWGYNIYGQLGFDGGNSATPCLLKQFLELEPPESLTEESGSKSKGSLKFPLLLYQICDI 151
           WCWGYN+YGQLGFDGGNSATPCLLKQFLELEPPESLTEESGSKSKGSLK       ICDI
Sbjct: 97  WCWGYNLYGQLGFDGGNSATPCLLKQFLELEPPESLTEESGSKSKGSLK-------ICDI 156

Query: 152 KAGGQISFAIDDLGALWMWGNCPKPSGENEVGLSYVSSFTPSPVWEFHGHTVVKVACGNE 211
           KAGGQISFAIDDLGALWMWGNCPKP+GENEV LSYVSSFTPSPVWEFHGHTVVKVACGNE
Sbjct: 157 KAGGQISFAIDDLGALWMWGNCPKPNGENEVSLSYVSSFTPSPVWEFHGHTVVKVACGNE 216

Query: 212 HIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACPQIIETFNEASPWAVYEVA 271
           HIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGD+DSRA PQIIETFNEASPWAVYE+A
Sbjct: 217 HIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDRDSRARPQIIETFNEASPWAVYEIA 276

Query: 272 CGAFHTALLSHSKRPCDTLESVCWTFGLGENGQLGHGTTQNEFLPQPVKELPQSVSLISV 331
           CGAFHTAL+S SKRPCDTLESVCWTFGLGENGQLGHGTTQNEFLPQPVKELP+SVSLISV
Sbjct: 277 CGAFHTALVSRSKRPCDTLESVCWTFGLGENGQLGHGTTQNEFLPQPVKELPKSVSLISV 336

Query: 332 ACGLFHTSVVSSGGDLWSWGMERGLGLCPDASFTGTNTGDAISPLSIFCSGTESPKFLDP 391
            CGLFHTSVVSSGGDLWSWGMERGLGLCPDASFTGTNTGDAISPLSIFCSG+ESPKFLDP
Sbjct: 337 DCGLFHTSVVSSGGDLWSWGMERGLGLCPDASFTGTNTGDAISPLSIFCSGSESPKFLDP 396

Query: 392 VQVACGAAHTVIVAHDGYKLWSWGRGRSGVLGNGKAVDCFTPTVVLWPPLNEDFDQEETE 451
           VQVACGAAHTVIVAHDGYKLWSWGRGRSGVLGNGKAVDCFTPTVVLWPPL+EDFDQEETE
Sbjct: 397 VQVACGAAHTVIVAHDGYKLWSWGRGRSGVLGNGKAVDCFTPTVVLWPPLDEDFDQEETE 456

Query: 452 TKCEEDKVEENKTEEDKVEENKIEEVTEENKKLLMAMEEIKLLLDKLSIMERYASFLHGS 511
           TK EEDKVEENKT          EEVTEENKKLLMAMEEIKLLL+KLSIMERYASFLHGS
Sbjct: 457 TKSEEDKVEENKT----------EEVTEENKKLLMAMEEIKLLLEKLSIMERYASFLHGS 516

Query: 512 IFGKPFQEGDIPLSLRNSGSFDIAKEWESMLESSDRSNLLRLETVYQNMLASIKDKILKK 571
           IFGKPFQEGDIPLSLRNSGSFDIAKEWESMLESSDRSNLLRLE VYQNMLASIKDKI+KK
Sbjct: 517 IFGKPFQEGDIPLSLRNSGSFDIAKEWESMLESSDRSNLLRLEVVYQNMLASIKDKIMKK 576

Query: 572 RVQELVKEFLQSATPPN 589
           RVQELVKEFLQS+T PN
Sbjct: 577 RVQELVKEFLQSSTSPN 576

BLAST of Cucsa.161260.1 vs. NCBI nr
Match: gi|1009135276|ref|XP_015884900.1| (PREDICTED: ultraviolet-B receptor UVR8 isoform X1 [Ziziphus jujuba])

HSP 1 Score: 839.0 bits (2166), Expect = 5.2e-240
Identity = 410/590 (69.49%), Postives = 477/590 (80.85%), Query Frame = 1

Query: 1   MEESGTRPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVR 60
           M++S   P +  LS KVVAI+AGEAHTL LTGDG +YSWGRG FGRLGTG+E+DELFPVR
Sbjct: 1   MDDSTNLPPADYLSRKVVAIAAGEAHTLALTGDGSIYSWGRGKFGRLGTGSEADELFPVR 60

Query: 61  VKL----GSGEENLKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLK 120
           VK     GS E  LK V +AAG+YHSLAL DDGSVWCWGYNIYGQLG +G NS  PCLL+
Sbjct: 61  VKFDIPHGSEERKLKFVGVAAGAYHSLALADDGSVWCWGYNIYGQLGVNGENSVVPCLLE 120

Query: 121 QFLELEPPESLTEESGSKSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKP 180
           QFLEL  P+SL +ES  KS+ SLK       +C +KAGG +S AID+LGALW+WGNCP+ 
Sbjct: 121 QFLELGNPDSLADESEVKSRASLK-------VCSVKAGGMMSLAIDNLGALWIWGNCPQQ 180

Query: 181 SGEN-EVGLSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWG 240
           S  + E G S VS+FTP+PVW+FHGH VVKVACGNEH+VALVS GETY K EDLVCYSWG
Sbjct: 181 SSSSTEDGFSLVSNFTPTPVWDFHGHIVVKVACGNEHVVALVSAGETY-KGEDLVCYSWG 240

Query: 241 DNSHGQLGLGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCW 300
            N+HGQLGLGD++SR  P I+ETFN+ SPWAVYEVACGAFHTALL+H KRP D LES CW
Sbjct: 241 SNNHGQLGLGDRESRHHPAIVETFNQDSPWAVYEVACGAFHTALLTHKKRPNDILESKCW 300

Query: 301 TFGLGENGQLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERG 360
           TFGLGENGQLGHGTTQN + P+PV ELP  V LISV CGLFHTSVVS+ GD+WSWGME+G
Sbjct: 301 TFGLGENGQLGHGTTQNAWSPEPVTELPSHVYLISVDCGLFHTSVVSTAGDVWSWGMEKG 360

Query: 361 LGLCPDASFTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWG 420
           LGLCPDASFTGT+ GDA+SPL I CSG   PKF DPVQ+ACGAAHTV+VA+DGYKLWSWG
Sbjct: 361 LGLCPDASFTGTDAGDALSPLPISCSGLNGPKFQDPVQIACGAAHTVLVANDGYKLWSWG 420

Query: 421 RGRSGVLGNGKAVDCFTPTVVLWPPLNEDFDQEETETKCEEDKVEENKTEEDKVEENKIE 480
           RGRSGVLGNGKA+DCF+ T+VLWPPL EDF QEE  T  +EDK  E   +   V EN   
Sbjct: 421 RGRSGVLGNGKAIDCFSSTLVLWPPLKEDFKQEELNTD-DEDKTAEK--DSKGVTEN--- 480

Query: 481 EVTEENKKLLMAMEEIKLLLDKLSIMERYASFLHGSIFGKPFQEGDIPLSLRNSGSFDIA 540
              E +K+L +AMEE+KLL  KLSIMERYAS LHGSIFGKP ++ DIP SL NS +FDIA
Sbjct: 481 ---ETDKRLSLAMEEVKLLKSKLSIMERYASTLHGSIFGKPLEQQDIPNSLHNSDTFDIA 540

Query: 541 KEWESMLESSDRSNLLRLETVYQNMLASIKDKILKKRVQELVKEFLQSAT 586
           KEWE+MLES+DR+ L+RLE  Y++MLA +KDK++K RVQE+++E+LQS+T
Sbjct: 541 KEWENMLESADRARLVRLEMFYRSMLAGVKDKLMKIRVQEIIREYLQSST 573

BLAST of Cucsa.161260.1 vs. NCBI nr
Match: gi|590573499|ref|XP_007012139.1| (Regulator of chromosome condensation family protein isoform 2 [Theobroma cacao])

HSP 1 Score: 824.7 bits (2129), Expect = 1.0e-235
Identity = 398/583 (68.27%), Postives = 470/583 (80.62%), Query Frame = 1

Query: 4   SGTRPSSGKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKL 63
           +GT P S  L  KVVA+SAGEAHTL L+GDG VYSWGRGMFGRLGTG+ESD  FPVRVK 
Sbjct: 5   AGTFPGSSNLFRKVVAVSAGEAHTLALSGDGCVYSWGRGMFGRLGTGSESDGHFPVRVKF 64

Query: 64  GSGEENLKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLELEP 123
            + E  LK VA+AAG+YHSLAL DDGSVW WGYNIYGQLG  G NS  P LL++FLEL  
Sbjct: 65  QNSE--LKFVAVAAGAYHSLALADDGSVWSWGYNIYGQLGVHGENSLAPQLLERFLELGS 124

Query: 124 PESLTEESGSKSKGSLKFPLLLYQICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVG 183
           P+   +E    +K  LK       IC +KAGG  S AID+LGALWMWG CP+ +  ++ G
Sbjct: 125 PDQSKDELEINTKTPLK-------ICAVKAGGMTSLAIDNLGALWMWGLCPQENSSSDGG 184

Query: 184 LSYVSSFTPSPVWEFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLG 243
           L++VSSF+P PVW+FHGHTVVKVACGNEH+VALVS GETY K +DLVCYSWG N HGQLG
Sbjct: 185 LTFVSSFSPIPVWDFHGHTVVKVACGNEHVVALVSAGETY-KGDDLVCYSWGGNGHGQLG 244

Query: 244 LGDKDSRACPQIIETFNEASPWAVYEVACGAFHTALLSHSKRPCDTLESVCWTFGLGENG 303
           LGD++SR  P+I+ETFN+  PW VYEVACGAFHTALL+H KRP +TLES+CWTFGLG+ G
Sbjct: 245 LGDRESRVHPEIVETFNQDIPWTVYEVACGAFHTALLTHRKRPSETLESMCWTFGLGDKG 304

Query: 304 QLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERGLGLCPDAS 363
           QLGHGTTQ+  +P+PVKELPQ V L+SV CGLFHTSVVSS GD+WSWGME+GLGLCPDAS
Sbjct: 305 QLGHGTTQSTLVPEPVKELPQPVYLVSVDCGLFHTSVVSSAGDVWSWGMEKGLGLCPDAS 364

Query: 364 FTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSGVLG 423
           FTGT+ GDA+SPL I   G   PKF DPVQ++CGAAHTV+VAHDGYKLWSWGRGRSGVLG
Sbjct: 365 FTGTDAGDALSPLQISGDGLHEPKFRDPVQISCGAAHTVVVAHDGYKLWSWGRGRSGVLG 424

Query: 424 NGKAVDCFTPTVVLWPPLNEDFDQEE-TETKCEEDKVEENKTEEDKVEENKIEEVTEENK 483
           NGK +DCFTP++VLWPPLNEDF QEE   T  E DK+ ++K           +  +E +K
Sbjct: 425 NGKTIDCFTPSMVLWPPLNEDFQQEELNSTVGEGDKIVDHKNS---------DAGSEMDK 484

Query: 484 KLLMAMEEIKLLLDKLSIMERYASFLHGSIFGKPFQEGDIPLSLRNSGSFDIAKEWESML 543
           KL +AMEE+KLL  KLSIMERYAS LHGSIFGKPF+E DIP+SL+NSG+FD+A+EWE+ML
Sbjct: 485 KLSLAMEEMKLLQSKLSIMERYASVLHGSIFGKPFEEQDIPISLQNSGTFDVAREWENML 544

Query: 544 ESSDRSNLLRLETVYQNMLASIKDKILKKRVQELVKEFLQSAT 586
           ESSDRS L+RLE  Y+NMLA +KDK++KKR+QE++KE+L S+T
Sbjct: 545 ESSDRSKLVRLELFYRNMLAGVKDKMMKKRIQEIIKEYLPSST 568

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
UVR8_ARATH1.8e-3528.81Ultraviolet-B receptor UVR8 OS=Arabidopsis thaliana GN=UVR8 PE=1 SV=1[more]
HERC4_MOUSE2.9e-2829.39Probable E3 ubiquitin-protein ligase HERC4 OS=Mus musculus GN=Herc4 PE=1 SV=2[more]
HERC4_HUMAN3.7e-2831.49Probable E3 ubiquitin-protein ligase HERC4 OS=Homo sapiens GN=HERC4 PE=1 SV=1[more]
HERC4_RAT6.4e-2828.82Probable E3 ubiquitin-protein ligase HERC4 OS=Rattus norvegicus GN=Herc4 PE=2 SV... [more]
HERC2_MOUSE8.3e-2828.97E3 ubiquitin-protein ligase HERC2 OS=Mus musculus GN=Herc2 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A0A0LPS2_CUCSA0.0e+0098.81Uncharacterized protein OS=Cucumis sativus GN=Csa_2G373550 PE=4 SV=1[more]
A0A061GK39_THECC7.1e-23668.27Regulator of chromosome condensation family protein isoform 2 OS=Theobroma cacao... [more]
A0A061GKT1_THECC3.9e-22665.55Regulator of chromosome condensation family protein isoform 1 OS=Theobroma cacao... [more]
F6H3D2_VITVI1.2e-21461.81Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0008g03320 PE=4 SV=... [more]
B9RRY8_RICCO4.5e-21464.13Protein pim1, putative OS=Ricinus communis GN=RCOM_0799870 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G11580.18.4e-18054.24 Regulator of chromosome condensation (RCC1) family protein[more]
AT5G63860.11.0e-3628.81 Regulator of chromosome condensation (RCC1) family protein[more]
AT5G08710.16.1e-2929.36 Regulator of chromosome condensation (RCC1) family protein[more]
AT5G16040.18.8e-2828.64 Regulator of chromosome condensation (RCC1) family protein[more]
AT1G69710.19.8e-2728.82 Regulator of chromosome condensation (RCC1) family with FYVE zinc fi... [more]
Match NameE-valueIdentityDescription
gi|778672037|ref|XP_004148391.2|0.0e+0098.81PREDICTED: ultraviolet-B receptor UVR8 [Cucumis sativus][more]
gi|659088493|ref|XP_008445010.1|0.0e+0092.86PREDICTED: probable E3 ubiquitin-protein ligase HERC4 isoform X2 [Cucumis melo][more]
gi|659088491|ref|XP_008445009.1|5.9e-30893.36PREDICTED: regulator of chromosome condensation isoform X1 [Cucumis melo][more]
gi|1009135276|ref|XP_015884900.1|5.2e-24069.49PREDICTED: ultraviolet-B receptor UVR8 isoform X1 [Ziziphus jujuba][more]
gi|590573499|ref|XP_007012139.1|1.0e-23568.27Regulator of chromosome condensation family protein isoform 2 [Theobroma cacao][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR000408Reg_chr_condens
IPR009091RCC1/BLIP-II
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Cucsa.161260Cucsa.161260gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Cucsa.161260.1Cucsa.161260.1-proteinpolypeptide


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.161260.1.five_prime_UTR.1Cucsa.161260.1.five_prime_UTR.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.161260.1.CDS.1Cucsa.161260.1.CDS.1CDS
Cucsa.161260.1.CDS.2Cucsa.161260.1.CDS.2CDS
Cucsa.161260.1.CDS.3Cucsa.161260.1.CDS.3CDS
Cucsa.161260.1.CDS.4Cucsa.161260.1.CDS.4CDS
Cucsa.161260.1.CDS.5Cucsa.161260.1.CDS.5CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Cucsa.161260.1.three_prime_UTR.1Cucsa.161260.1.three_prime_UTR.1three_prime_UTR


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000408Regulator of chromosome condensation, RCC1PRINTSPR00633RCCNDNSATIONcoord: 334..352
score: 2.8E-18coord: 91..107
score: 2.8E-18coord: 35..51
score: 2.8E-18coord: 72..85
score: 2.8E-18coord: 206..222
score: 2.8E-18coord: 404..425
score: 2.8
IPR000408Regulator of chromosome condensation, RCC1PFAMPF00415RCC1coord: 295..340
score: 2.4E-10coord: 33..85
score: 1.6E-15coord: 88..161
score: 1.3E-5coord: 232..280
score: 2.0
IPR000408Regulator of chromosome condensation, RCC1PROSITEPS00626RCC1_2coord: 270..280
score: -coord: 206..216
score: -coord: 75..85
scor
IPR000408Regulator of chromosome condensation, RCC1PROFILEPS50012RCC1_3coord: 34..88
score: 17.72coord: 229..283
score: 14.926coord: 89..164
score: 9.057coord: 345..407
score: 8.615coord: 165..219
score: 10.444coord: 292..344
score: 13
IPR009091Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein IIGENE3DG3DSA:2.130.10.30coord: 15..159
score: 8.7E-38coord: 160..439
score: 1.9
IPR009091Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein IIunknownSSF50985RCC1/BLIP-IIcoord: 147..320
score: 2.27E-54coord: 15..110
score: 2.27E-54coord: 267..436
score: 1.94
NoneNo IPR availableunknownCoilCoilcoord: 474..494
scor
NoneNo IPR availablePANTHERPTHR22870REGULATOR OF CHROMOSOME CONDENSATIONcoord: 1..584
score: 1.7E
NoneNo IPR availablePANTHERPTHR22870:SF155SUBFAMILY NOT NAMEDcoord: 1..584
score: 1.7E