Cucsa.049550.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.049550.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	RING/U-box superfamily protein
Location	scaffold00550 : 29154 .. 32519 (+)
Sequence length	2477

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCTTCACCAGGACCACCAATATGCATTACTACTGGTGCCGAATTTGTCGAAGAATCATCAGAATTAGCTTAGGAAACCCTTTAGAAATTTCCATTACTTGCCCCTTTTGCTCCAGAAATCTCCGCCATGAATTGGACGTAGCCAGGGCTCGAAATTTCCCTCGTCTCCCCGCCATTTCCCCTCCGATTGTCCAACGGTGGGGATTCGAAGAAACCGATGCGTCATGGATCACGCTCCAATTCCCGAGACCAGCGGTAAATTCCGGGATTGAGGAGATTCCAAGGGTGAGAATTACGGGGAAGCATTTGGAGAAGGATTCGAATTGTGCGATTTGTAAAGAGGAATTTGAAATGGGAGAGGAAGTTAGGGAGTTGCCTTGCAAGCATTTTTACCATTCGGATTGTGTTGTTCCTTGGTTGAGAATGCATAACACTTGCCCTGTTTGCCGGTATACGTTGGAGAATATCGGCGACGAGGGGAGTGGGAGCGGTTTTAATGGCGGAAGGGGTGAGGAATTCCGGGAAGAAGAAGAAGAAGGTGAAAGAAGAGGGAATGGATGGTGGAACTTGGTGTGTTGCTGGTGGCCGTTCCGTGTAGTCGGCGATTGGGCTCGCCGTCTCCGGTTTGCTGATTCGGGTGATAGTTTCTTCGCCGTTGAACGTAAGGTTTTTtCTTTtCAAGTTTCTTCAGAGATGGATGGAGTTGGGAAACAAAATATAAATAACAAACCATTTTTTtATAATCAAAATGAAATCGTTGAGATTATTACAATTATTTTAAATTTTATCTTTAATTTTGTAATTTTAGATTAGATTACTGTGTTTTGAATTCTCTTGTAACCATTTATATTTTTtACTTTTGCTGAGAATTTTTCAAAAAAAAAAAaaTACAAACTACACAATTTATAAACACAACTCCGTATTTGATAAAAGATTAAATGTTAATTTGTATTGGTAGAGTTTGACATAAAATGTCTAATTTCAATCTATATACTTTGGTAGTTTAATATCTACTAGCATTCTAACCATAAATTTTAATAACACATTCACGTAAATATTATAAATATTATTATTATTGTCAAACCAAATAGAATAAAAAATAATTTTGAGAACTATATTAAAAATGTATTGAATACATTCAAACAAATACTAAAATATAATTTTCTTAAAAAaCAAAAAAGTGACAGAAATCCATGAAGATCCTTACACAAGACAACTAGATTCAAAATGACAAAATGGACCATCAAAATCAAACAAGTCAACTTAGAAAAGGACAAAATTTGACCATCAAAATCAAACTAATCAAATTCACAAAAACATAACTAAACTCTAACTCAAAACAATCAAATAGACAAAAAAAAAAATAATAAAAAAAAAAaGaGTTTGCAAAATCTAAATATTTAAATCTAATGTTAATTAATGTGTACTGGTTGGAGTTTGCAGCAGGTTCGTGGATGTACTCTTGGCTAGCCAATTAACCCACCCCCGGTAATATCTACTACATTCCTTACTCTTCATTTCCTTTCATTTCTTAACCAATTGATTAGGTACATGAAATTGTGTTGGATTTAGTAATGTAATTAACAAGCATTTTGCTTTCTTATTCCATTTCTTTCTTGTAAGTAACTACCAAAATTGCAGACAGTCAAAAGGAACCTTTGTTTAAGAACTGTACATCTTTTACTAAAGAAAATAAGACTATATAATAATAGTTCCTTTTGAGGTCTGCAACTTCAAATCTCTCCTTTCATAAATTAATTATATTTTTTtCTAAAACATATATAATTTAAACCCAACCAAGGAGGAGAGTTGGTTCCCTTTGGAAAACAACATCCCTATTTCCCTGCGTAGGGTTAATATAAAGGGAAATATCACCTTTTGTCTTCTTCTTATTCTTCTTCTTATTCTTCTTCGGACTCTCTATACTGACTTTCTTTGTTCTTGAGGAGTGAACCAAAAATGAGTTTCCCATCTGATTGTATTCTTCAGACTATTGTATGTTGATTATGTAGGTGATAGATGTAAATGAGTTGTAGGAGAGAGCGGTCAAGACCCGTTATTCTTAATCCTACAAAGATGAGAAGGGAATGAGTTGTGCTTTTGTAGATATAAATGAATTGATTTTGTACTAAGAAATACTACTACTACGTGCTTTCAACCTATAATCAAGTAAAAAATATGTCAAACCTTACATGAACTGGATGTGACGGATCTTCATTTGTCTGCCCTGATTTGGAGCGAAAAGAAGGCTTGGGTTTAGGTAACAGATCGGGGGGCAACAGTGGTGGAGACTCTACTGGAGATGCACGTGGAGAATAAACACGAGGTTGATTAGCTGGAGGAACGATCAGTCTGGTAGACTTAGGATACGAGATTGGTGGTATTGAATGTTCATGAGGGGAATTTGCAGGAACCATAGAAGGGTTCGGAGACGAGCTTTTGCAATTTGCATGAGGTGGGGAATGGTTTGCTGGTGGTCGCATGGATCTCAACGGATGTGGGCCACTCGGTACTTCTACATGATTGCCAGGCGCTGGGGCAGGGGCTGGAGAAAAACTTGGTGGCATTGCCTTGGAGGTTCTCTTCGGATAAGAAGACAAACTGACACTCTTGACTTCTCCAAAAACAGAATAATCAAGGCCAAGATTTCTTTCAGGAGAGGTGTTGATGATTGCAGCCAACTGCTGTAATCTCTGTGACGTAATGCGTCCCAATTCCGACGTAATAGAAGCCTGAACAATTACGAGTGGTTGCACTGTCGAGCCAATCTTGTTTGTTATTTGCAAATACACATTCTGTATAACAAGAAAAGAAAGAGTAGAATCCAACATCCATTAATCAACTCCAGTCCCAGATACATAAAACTAACAACAATAATATAAAACTCATCTCAAGTCTAAACTTATGATCTCAAGTTCTAGAGATAATATTTGATAAATTCCATCCATAGCAGAAAGTATAAGTTTAACTTAAATAAGTTTTTGTACCTCATAAGACCTCAGACGCAATCCAAACTTTAGCTGGCTCTTGAACTTGGCAAAGTTGTCAAGTATTTCGGAAATGGAGTTAGTAAGAGTGAAGTTAAATACGATCTGGGGAAACTCCCAAATCGAAGCATGTTGAAATGGGATTATAGAAATCCCCCCTGGAAACTTGAGAATTTGAAGTGTCGATGGCTGTCCAAAAATTGATGTCGTCAAAGTAAGGTTGGATTCCGAAAGGAAAAAGTCATATAAAGATGATCTCAGCAGACTTAAGGACACTGGATTTATTGGAGCAGTTATGTATTCAGAAAGAAGACCGAAAACCACGTAAGTCCTGTTTGACTCACCTATATCATGCATGGATAGAATGGAAACCT

mRNA sequence

ATGGCGGCTTCACCAGGACCACCAATATGCATTACTACTGCCAGGGCTCGAAATTTCCCTCGTCTCCCCGCCATTTCCCCTCCGATTGTCCAACGGTGGGGATTCGAAGAAACCGATGCGTCATGGATCACGCTCCAATTCCCGAGACCAGCGGTAAATTCCGGGATTGAGGAGATTCCAAGGGTGAGAATTACGGGGAAGCATTTGGAGAAGGATTCGAATTGTGCGATTTGTAAAGAGGAATTTGAAATGGGAGAGGAAGTTAGGGAGTTGCCTTGCAAGCATTTTTACCATTCGGATTGTGTTGTTCCTTGGTTGAGAATGCATAACACTTGCCCTGTTTGCCGGTATACGTTGGAGAATATCGGCGACGAGGGGAGTGGGAGCGGTTTTAATGGCGGAAGGGGTGAGGAATTCCGGGAAGAAGAAGAAGAAGGTGAAAGAAGAGGGAATGGATGGTGGAACTTGGTGTGTTGCTGGTGGCCGTTCCGTGTAGTCGGCGATTGGGCTCGCCGTCTCCGGTTTGCTGATTCGGGTGATAGTTTCTTCGCCGTTGAACCAGGTTCGTGGATGTACTCTTGGCTAGCCAATTAACCCACCCCCGGTAATATCTACTACATTCCTTACTCTTCATTTCCTTTCATTTCTTAACCAATTGATTAGGTACATGAAATTGTGTTGGATTTAGTAATGTAATTAACAAGCATTTTGCTTTCTTATTCCATTTCTTTCTTGTAAGTAACTACCAAAATTGCAGACAGTCAAAAGGAACCTTTGTTTAAGAACTGTACATCTTTTACTAAAGAAAATAAGACTATATAATAATAGTTCCTTTTGAGGTCTGCAACTTCAAATCTCTCCTTTCataaattaattatatttttttctaaaacatatataatttaaaCCCAACCAAGGAGGAGAGTTGGTTCCCTTTGGAAAACAACATCCCTATTTCCCTGCGTAGGGTTAATATAAAGGGAAATATCACCTTTTGtcttcttcttattcttcttcttattcttcttcGGACTCTCTATACTGACTTTCTTTGTTCTTGAGGAGTGAACCAAAAATGAGTTTCCCATCTGATTGTATTCTTCAGACTATTGTATGTTGATTATGTAGGTGATAGATGTAAATGAGTTGTAGGAGAGAGCGGTCAAGACCCGTTATTCTTAATCCTACAAAGATGAGAAGGGAATGAGTTGTGCTTTTGTAGATATAAATGAATTGATTTTGTACTAAGAAATACTACTACTACGTGCTTTCAACCTATAATCAAGTAAAAAATATGTCAAACCTTACATGAACTGGATGTGACGGATCTTCATTTGTCTGCCCTGATTTGGAGCGAAAAGAAGGCTTGGGTTTAGGTAACAGATCGGGGGGCAACAGTGGTGGAGACTCTACTGGAGATGCACGTGGAGAATAAACACGAGGTTGATTAGCTGGAGGAACGATCAGTCTGGTAGACTTAGGATACGAGATTGGTGGTATTGAATGTTCATGAGGGGAATTTGCAGGAACCATAGAAGGGTTCGGAGACGAGCTTTTGCAATTTGCATGAGGTGGGGAATGGTTTGCTGGTGGTCGCATGGATCTCAACGGATGTGGGCCACTCGGTACTTCTACATGATTGCCAGGCGCTGGGGCAGGGGCTGGAGAAAAACTTGGTGGCATTGCCTTGGAGGTTCTCTTCGGATAAGAAGACAAACTGACACTCTTGACTTCTCCAAAAACAGAATAATCAAGGCCAAGATTTCTTTCAGGAGAGGTGTTGATGATTGCAGCCAACTGCTGTAATCTCTGTGACGTAATGCGTCCCAATTCCGACGTAATAGAAGCCTGAACAATTACGAGTGGTTGCACTGTCGAGCCAATCTTGTTTGTTATTTGCAAATACACATTCTGTATAACAAGAAAAGAAAGAGTAGAATCCAACATCCATTAATCAACTCCAGTCCCAGATACATAAAACTAACAACAATAATATAAAACTCATCTCAAGTCTAAACTTATGATCTCAAGTTCTAGAGATAATATTTGATAAATTCCATCCATAGCAGAAAGTATAAGTTTAACTTAAATAAGTTTTTGTACCTCATAAGACCTCAGACGCAATCCAAACTTTAGCTGGCTCTTGAACTTGGCAAAGTTGTCAAGTATTTCGGAAATGGAGTTAGTAAGAGTGAAGTTAAATACGATCTGGGGAAACTCCCAAATCGAAGCATGTTGAAATGGGATTATAGAAATCCCCCCTGGAAACTTGAGAATTTGAAGTGTCGATGGCTGTCCAAAAATTGATGTCGTCAAAGTAAGGTTGGATTCCGAAAGGAAAAAGTCATATAAAGATGATCTCAGCAGACTTAAGGACACTGGATTTATTGGAGCAGTTATGTATTCAGAAAGAAGACCGAAAACCACGTAAGTCCTGTTTGACTCACCTATATCATGCATGGATAGAATGGAAACCT

Coding sequence (CDS)

ATGGCGGCTTCACCAGGACCACCAATATGCATTACTACTGCCAGGGCTCGAAATTTCCCTCGTCTCCCCGCCATTTCCCCTCCGATTGTCCAACGGTGGGGATTCGAAGAAACCGATGCGTCATGGATCACGCTCCAATTCCCGAGACCAGCGGTAAATTCCGGGATTGAGGAGATTCCAAGGGTGAGAATTACGGGGAAGCATTTGGAGAAGGATTCGAATTGTGCGATTTGTAAAGAGGAATTTGAAATGGGAGAGGAAGTTAGGGAGTTGCCTTGCAAGCATTTTTACCATTCGGATTGTGTTGTTCCTTGGTTGAGAATGCATAACACTTGCCCTGTTTGCCGGTATACGTTGGAGAATATCGGCGACGAGGGGAGTGGGAGCGGTTTTAATGGCGGAAGGGGTGAGGAATTCCGGGAAGAAGAAGAAGAAGGTGAAAGAAGAGGGAATGGATGGTGGAACTTGGTGTGTTGCTGGTGGCCGTTCCGTGTAGTCGGCGATTGGGCTCGCCGTCTCCGGTTTGCTGATTCGGGTGATAGTTTCTTCGCCGTTGAACCAGGTTCGTGGATGTACTCTTGGCTAGCCAATTAA

Protein sequence

MAASPGPPICITTARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSGFNGGRGEEFREEEEEGERRGNGWWNLVCCWWPFRVVGDWARRLRFADSGDSFFAVEPGSWMYSWLAN*

Homology

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BLAST of Cucsa.049550.1 vs. Swiss-Prot
Match: RHC1A_ARATH (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana GN=RHC1A PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.5e-23
Identity = 54/141 (38.30%), Postives = 73/141 (51.77%), Query Frame = 1


Query: 35  FEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLEK-DSNCAICKEEFEMGEEVRELPC 94
           FE+  A   T + P PA  S I+ +P ++I  +HL   DSNC +CK+EFE+G E +++PC
Sbjct: 150 FEQLSAG-TTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPC 209

Query: 95  KHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGS-------GFNGGRGEEFREEEEEG 154
            H YHSDC+VPWL  HN+CPVCR  L +     S          +            E G
Sbjct: 210 NHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRENG 269

Query: 155 ERRGNGWWNLVCCWWPFRVVG 168
             R N +      +WPFR  G
Sbjct: 270 NERRNPF----SSFWPFRSSG 285

[Close]

BLAST of Cucsa.049550.1 vs. Swiss-Prot
Match: RDUF1_ARATH (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 8.1e-20
Identity = 50/114 (43.86%), Postives = 64/114 (56.14%), Query Frame = 1


Query: 50  PAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMH 109
           PA  S IE +PRV I+  H + ++NCA+C E FE G E RE+PCKH +H DC+VPWL + 
Sbjct: 190 PASKSAIESLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIR 249

Query: 110 NTCPVCRYTLENIGDEGS---------------GSGFNGGRGEEFREEEEEGER 149
           N+CPVCR+ L +   + S               G GF  GR   F     EGER
Sbjct: 250 NSCPVCRFELPSDPIQRSNEEEHAVGMTIWRLPGGGFAVGR---FNAGVREGER 300

[Close]

BLAST of Cucsa.049550.1 vs. Swiss-Prot
Match: RING1_GOSHI (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.4e-19
Identity = 46/124 (37.10%), Postives = 68/124 (54.84%), Query Frame = 1


Query: 50  PAVNSGIEEIPRVRITGKHLEKDSN-CAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRM 109
           PA  S IE +P V IT  +L  + N CA+C ++FE G E +++PCKH YH DC++PWL +
Sbjct: 198 PASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLEL 257

Query: 110 HNTCPVCRYTLENIGDE-----GSGSGFNGGRGEEFREEEEEGERRGNGWWNLVCCWWPF 168
           HN+CPVCR+ L     +         G +GG   +   +  +G+ R       +   WPF
Sbjct: 258 HNSCPVCRHELPTDDPDYERRVRGAQGTSGGNDGDNSGQRSDGDNRTVERSFRISLPWPF 317

[Close]

BLAST of Cucsa.049550.1 vs. Swiss-Prot
Match: RDUF2_ARATH (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana GN=DURF2 PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.8e-19
Identity = 52/129 (40.31%), Postives = 68/129 (52.71%), Query Frame = 1


Query: 37  ETDASWITLQFPRPAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHF 96
           E  A+ I      PA  S IE +PRV I+  H+  ++NCA+C E FE   E RE+PCKH 
Sbjct: 161 EASATGIGRSGNPPASKSAIESLPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHL 220

Query: 97  YHSDCVVPWLRMHNTCPVCRYTL-------ENIGDEGS----------GSGFNGGRGEEF 149
           +H DC+VPWL + N+CPVCR+ L        N  +E +          G GF  GR   F
Sbjct: 221 FHDDCIVPWLSIRNSCPVCRFELPSEPNRRSNNNEEDNAVGMTIWRLPGGGFAVGR---F 280

[Close]

BLAST of Cucsa.049550.1 vs. Swiss-Prot
Match: RNG1L_ARATH (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 3.1e-19
Identity = 41/99 (41.41%), Postives = 63/99 (63.64%), Query Frame = 1


Query: 50  PAVNSGIEEIPRVRITGKHLEKDSN-CAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRM 109
           PA  S I+ +P V++T   L+ + N CA+C +EFE G +V+++PCKH +H DC++PWL +
Sbjct: 190 PASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLEL 249

Query: 110 HNTCPVCRYTL---------ENIGDEGSGSGFNGGRGEE 139
           HN+CPVCR+ L          + G +GSG G     G++
Sbjct: 250 HNSCPVCRFELPTDDPDYENRSQGSQGSGDGQGSVEGQQ 288

[Close]

BLAST of Cucsa.049550.1 vs. TrEMBL
Match: A0A0A0LC10_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G214060 PE=4 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 1.2e-102
Identity = 173/182 (95.05%), Postives = 176/182 (96.70%), Query Frame = 1


Query: 11  ITTARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 70
           +  ARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE
Sbjct: 62  LDVARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 121

Query: 71  KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSG 130
           KDSNCAICKEEFEMGEEVRELPCKHFYHSDCV+PWLRMHNTCPVCRYTLENIGDEGSGSG
Sbjct: 122 KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVIPWLRMHNTCPVCRYTLENIGDEGSGSG 181

Query: 131 FNGGRGEEFR-EEEEEGERRGNGWWNLVCCWWPFRVVGDWARRLRFADSGDSFFAVEPGS 190
           FNGGRGEEFR EEEEEGERRGNGWWNLVCCWWPFR+VGDWARRLRFADSGDSFFAVE   
Sbjct: 182 FNGGRGEEFREEEEEEGERRGNGWWNLVCCWWPFRLVGDWARRLRFADSGDSFFAVERKR 241

Query: 191 WM 192
           WM
Sbjct: 242 WM 243

[Close]

BLAST of Cucsa.049550.1 vs. TrEMBL
Match: A0A068TNL0_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00014914001 PE=4 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 3.9e-29
Identity = 62/125 (49.60%), Postives = 79/125 (63.20%), Query Frame = 1


Query: 48  PRPAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLR 107
           P PA  S I+ +P + +T  HL  DS+C +CK+EFE GEEVRELPCKHFYHSDC++PWL 
Sbjct: 157 PTPA--SAIDALPTIVLTPTHLAIDSHCPVCKDEFEAGEEVRELPCKHFYHSDCIIPWLH 216

Query: 108 MHNTCPVCRYTLENIGDEGSGSGFNGGRGEEFREEEEEGERRGNGWWNLVCCWWPFRVVG 167
           + N+CPVCRY +  +    S S F   + + F+EEEE        W   V   WPF + G
Sbjct: 217 IRNSCPVCRYQIRGL----SNSDFREEQADNFQEEEEVSSSPHRNWLLRVFSLWPFSLFG 275

Query: 168 DWARR 173
           DWA R
Sbjct: 277 DWAYR 275

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BLAST of Cucsa.049550.1 vs. TrEMBL
Match: M1C6Z4_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400023756 PE=4 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.1e-28
Identity = 62/128 (48.44%), Postives = 81/128 (63.28%), Query Frame = 1


Query: 48  PRPAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLR 107
           P P  +S I+ +PR+ +T   LE DS C +CK+EFE+G EV ELPCKHFYHS+C+VPWLR
Sbjct: 150 PPPTPSSAIDALPRIYLTPNLLENDSVCPVCKDEFEVGIEVIELPCKHFYHSECIVPWLR 209

Query: 108 MHNTCPVCRYTLENIGDEGSGSGFNGGRGEEFREEEEEGERRGNG--W-WNLVCCWWPFR 167
           +HNTCPVCRY L+   +    +G+      EF E+EEE E   N   W W  +   WPF 
Sbjct: 210 LHNTCPVCRYQLQGFSNNEDQNGYT----YEFNEQEEEEENIQNPLIWGWTQLTSLWPFS 269

Query: 168 VVGDWARR 173
           ++  W  R
Sbjct: 270 LMSSWRHR 273

[Close]

BLAST of Cucsa.049550.1 vs. TrEMBL
Match: A0A0B2RNN4_GLYSO (E3 ubiquitin-protein ligase RING1 OS=Glycine soja GN=glysoja_005509 PE=4 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 7.4e-28
Identity = 60/125 (48.00%), Postives = 75/125 (60.00%), Query Frame = 1


Query: 48  PRPAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLR 107
           P PA +S I  +P V++T  HL  D NC ICK+EFE+  E RELPCKHFYHSDC++PWLR
Sbjct: 172 PPPATSSAIAALPMVKLTQTHLASDPNCPICKDEFELDMEARELPCKHFYHSDCIIPWLR 231

Query: 108 MHNTCPVCRYTLENIGDEGSGSGFNGGRGEEFREEEEEGERRGNGW-WNLVCCWWPFRVV 167
           MHNTCPVCRY L+ +   G+ + +     E       E       W WN +  + P R V
Sbjct: 232 MHNTCPVCRYELQGVSTSGNANYYRLQNDENDMRFGFEDAASSLVWVWNQLTSFRPIRSV 291

Query: 168 GDWAR 172
            DW R
Sbjct: 292 LDWTR 296

[Close]

BLAST of Cucsa.049550.1 vs. TrEMBL
Match: K7M9N3_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_15G047300 PE=4 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 7.4e-28
Identity = 60/125 (48.00%), Postives = 75/125 (60.00%), Query Frame = 1


Query: 48  PRPAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLR 107
           P PA +S I  +P V++T  HL  D NC ICK+EFE+  E RELPCKHFYHSDC++PWLR
Sbjct: 172 PPPATSSAIAALPMVKLTQTHLASDPNCPICKDEFELDMEARELPCKHFYHSDCIIPWLR 231

Query: 108 MHNTCPVCRYTLENIGDEGSGSGFNGGRGEEFREEEEEGERRGNGW-WNLVCCWWPFRVV 167
           MHNTCPVCRY L+ +   G+ + +     E       E       W WN +  + P R V
Sbjct: 232 MHNTCPVCRYELQGVSTSGNANYYRLQNDENDMRFGFEDAASSLVWVWNQLTSFRPIRSV 291

Query: 168 GDWAR 172
            DW R
Sbjct: 292 LDWTR 296

[Close]

BLAST of Cucsa.049550.1 vs. TAIR10
Match: AT1G68180.1 (AT1G68180.1 RING/U-box superfamily protein)

HSP 1 Score: 112.1 bits (279), Expect = 4.0e-25
Identity = 57/132 (43.18%), Postives = 71/132 (53.79%), Query Frame = 1


Query: 48  PRPAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLR 107
           P PA  S IE +  V IT + L K+  CAICKEEFE+GEE +EL C H YHS C+V WL 
Sbjct: 111 PPPASQSAIEAVRTVIITDEDLVKEKVCAICKEEFEVGEEGKELKCLHLYHSSCIVSWLN 170

Query: 108 MHNTCPVCRYTLENIG------DEGSGSGFNGGRGEEFREEEEEGERRGNGWWNLVCCWW 167
           +HNTCP+CR+ + N+G      DEG     +  R   FR                VC  W
Sbjct: 171 IHNTCPICRFEV-NLGVSESNVDEGGSYNIDNDRSNRFRTR--------------VCSLW 227

Query: 168 PFRVVGDWARRL 174
           P R++ DW   L
Sbjct: 231 PLRMMIDWVHNL 227

[Close]

BLAST of Cucsa.049550.1 vs. TAIR10
Match: AT2G40830.2 (AT2G40830.2 RING-H2 finger C1A)

HSP 1 Score: 109.8 bits (273), Expect = 2.0e-24
Identity = 54/141 (38.30%), Postives = 73/141 (51.77%), Query Frame = 1


Query: 35  FEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLEK-DSNCAICKEEFEMGEEVRELPC 94
           FE+  A   T + P PA  S I+ +P ++I  +HL   DSNC +CK+EFE+G E +++PC
Sbjct: 150 FEQLSAG-TTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPC 209

Query: 95  KHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGS-------GFNGGRGEEFREEEEEG 154
            H YHSDC+VPWL  HN+CPVCR  L +     S          +            E G
Sbjct: 210 NHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNYRSSSSSSSSNSRENG 269

Query: 155 ERRGNGWWNLVCCWWPFRVVG 168
             R N +      +WPFR  G
Sbjct: 270 NERRNPF----SSFWPFRSSG 285

[Close]

BLAST of Cucsa.049550.1 vs. TAIR10
Match: AT3G56580.2 (AT3G56580.2 RING/U-box superfamily protein)

HSP 1 Score: 107.8 bits (268), Expect = 7.6e-24
Identity = 52/118 (44.07%), Postives = 69/118 (58.47%), Query Frame = 1


Query: 48  PRPAVNSGIEEIPRVRITGKHLEK-DSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWL 107
           P PA  S I+ +P ++IT KHL+  DS+C +CK+EFE+  E +++PC H YHSDC+VPWL
Sbjct: 158 PPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWL 217

Query: 108 RMHNTCPVCRYTLENIGDEGSGSGFNGGRGEEFREEEEEGERRGNGWWNLVCCWWPFR 165
             HN+CPVCR  L + G   S         +       E  RR N + NL    WPFR
Sbjct: 218 VQHNSCPVCRKELPSRGSSSSTQS-----SQNRSTNGRENSRRRNIFSNL----WPFR 266

[Close]

BLAST of Cucsa.049550.1 vs. TAIR10
Match: AT3G46620.1 (AT3G46620.1 zinc finger (C3HC4-type RING finger) family protein)

HSP 1 Score: 98.6 bits (244), Expect = 4.6e-21
Identity = 50/114 (43.86%), Postives = 64/114 (56.14%), Query Frame = 1


Query: 50  PAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMH 109
           PA  S IE +PRV I+  H + ++NCA+C E FE G E RE+PCKH +H DC+VPWL + 
Sbjct: 190 PASKSAIESLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIR 249

Query: 110 NTCPVCRYTLENIGDEGS---------------GSGFNGGRGEEFREEEEEGER 149
           N+CPVCR+ L +   + S               G GF  GR   F     EGER
Sbjct: 250 NSCPVCRFELPSDPIQRSNEEEHAVGMTIWRLPGGGFAVGR---FNAGVREGER 300

[Close]

BLAST of Cucsa.049550.1 vs. TAIR10
Match: AT5G59550.1 (AT5G59550.1 zinc finger (C3HC4-type RING finger) family protein)

HSP 1 Score: 97.4 bits (241), Expect = 1.0e-20
Identity = 52/129 (40.31%), Postives = 68/129 (52.71%), Query Frame = 1


Query: 37  ETDASWITLQFPRPAVNSGIEEIPRVRITGKHLEKDSNCAICKEEFEMGEEVRELPCKHF 96
           E  A+ I      PA  S IE +PRV I+  H+  ++NCA+C E FE   E RE+PCKH 
Sbjct: 161 EASATGIGRSGNPPASKSAIESLPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHL 220

Query: 97  YHSDCVVPWLRMHNTCPVCRYTL-------ENIGDEGS----------GSGFNGGRGEEF 149
           +H DC+VPWL + N+CPVCR+ L        N  +E +          G GF  GR   F
Sbjct: 221 FHDDCIVPWLSIRNSCPVCRFELPSEPNRRSNNNEEDNAVGMTIWRLPGGGFAVGR---F 280

[Close]

BLAST of Cucsa.049550.1 vs. NCBI nr
Match: gi|449464484|ref|XP_004149959.1| (PREDICTED: E3 ubiquitin-protein ligase RNF126 isoform X1 [Cucumis sativus])

HSP 1 Score: 402.9 bits (1034), Expect = 3.2e-109
Identity = 182/188 (96.81%), Postives = 185/188 (98.40%), Query Frame = 1


Query: 11  ITTARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 70
           +  ARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE
Sbjct: 62  LDVARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 121

Query: 71  KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSG 130
           KDSNCAICKEEFEMGEEVRELPCKHFYHSDCV+PWLRMHNTCPVCRYTLENIGDEGSGSG
Sbjct: 122 KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVIPWLRMHNTCPVCRYTLENIGDEGSGSG 181

Query: 131 FNGGRGEEFR-EEEEEGERRGNGWWNLVCCWWPFRVVGDWARRLRFADSGDSFFAVEPGS 190
           FNGGRGEEFR EEEEEGERRGNGWWNLVCCWWPFR+VGDWARRLRFADSGDSFFAVEPGS
Sbjct: 182 FNGGRGEEFREEEEEEGERRGNGWWNLVCCWWPFRLVGDWARRLRFADSGDSFFAVEPGS 241

Query: 191 WMYSWLAN 198
           WMYSWLAN
Sbjct: 242 WMYSWLAN 249

[Close]

BLAST of Cucsa.049550.1 vs. NCBI nr
Match: gi|778680175|ref|XP_011651263.1| (PREDICTED: E3 ubiquitin-protein ligase RNF126 isoform X2 [Cucumis sativus])

HSP 1 Score: 392.5 bits (1007), Expect = 4.4e-106
Identity = 180/188 (95.74%), Postives = 183/188 (97.34%), Query Frame = 1


Query: 11  ITTARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 70
           +  ARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE
Sbjct: 62  LDVARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 121

Query: 71  KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSG 130
           KDSNCAICKEEFEMGEEVRELPCKHFYHSDCV+PWLRMHNTCPVCRYTLENIGDEGSGSG
Sbjct: 122 KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVIPWLRMHNTCPVCRYTLENIGDEGSGSG 181

Query: 131 FNGGRGEEFR-EEEEEGERRGNGWWNLVCCWWPFRVVGDWARRLRFADSGDSFFAVEPGS 190
           FNGGRGEEFR EEEEEGERRGNGWWNLVCCWWPFR+VGDWARRLRFADSGDSFFAVE  S
Sbjct: 182 FNGGRGEEFREEEEEEGERRGNGWWNLVCCWWPFRLVGDWARRLRFADSGDSFFAVE-RS 241

Query: 191 WMYSWLAN 198
           WMYSWLAN
Sbjct: 242 WMYSWLAN 248

[Close]

BLAST of Cucsa.049550.1 vs. NCBI nr
Match: gi|659112139|ref|XP_008456082.1| (PREDICTED: uncharacterized protein LOC103496124 isoform X1 [Cucumis melo])

HSP 1 Score: 384.8 bits (987), Expect = 9.1e-104
Identity = 174/192 (90.62%), Postives = 178/192 (92.71%), Query Frame = 1


Query: 11  ITTARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 70
           +  ARARNFPRLP ISPPIVQRWGFE+TD SWITLQFPRPAVNSGIEEIPRVRITGKHLE
Sbjct: 62  LDVARARNFPRLPPISPPIVQRWGFEDTDESWITLQFPRPAVNSGIEEIPRVRITGKHLE 121

Query: 71  KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSG 130
           KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSG
Sbjct: 122 KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSG 181

Query: 131 FNGGRGEEFREEEE-----EGERRGNGWWNLVCCWWPFRVVGDWARRLRFADSGDSFFAV 190
           FNGGRGEE  EEEE     EGE RGNGWWNLVCCWWP R++GDWARRLRFADSGDSFF V
Sbjct: 182 FNGGRGEELGEEEEEEEEGEGEGRGNGWWNLVCCWWPLRLIGDWARRLRFADSGDSFFVV 241

Query: 191 EPGSWMYSWLAN 198
           EPGSWMYSWLAN
Sbjct: 242 EPGSWMYSWLAN 253

[Close]

BLAST of Cucsa.049550.1 vs. NCBI nr
Match: gi|778680186|ref|XP_011651266.1| (PREDICTED: E3 ubiquitin-protein ligase RNF126 isoform X3 [Cucumis sativus])

HSP 1 Score: 381.3 bits (978), Expect = 1.0e-102
Identity = 173/179 (96.65%), Postives = 176/179 (98.32%), Query Frame = 1


Query: 11  ITTARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 70
           +  ARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE
Sbjct: 62  LDVARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 121

Query: 71  KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSG 130
           KDSNCAICKEEFEMGEEVRELPCKHFYHSDCV+PWLRMHNTCPVCRYTLENIGDEGSGSG
Sbjct: 122 KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVIPWLRMHNTCPVCRYTLENIGDEGSGSG 181

Query: 131 FNGGRGEEFR-EEEEEGERRGNGWWNLVCCWWPFRVVGDWARRLRFADSGDSFFAVEPG 189
           FNGGRGEEFR EEEEEGERRGNGWWNLVCCWWPFR+VGDWARRLRFADSGDSFFAVEPG
Sbjct: 182 FNGGRGEEFREEEEEEGERRGNGWWNLVCCWWPFRLVGDWARRLRFADSGDSFFAVEPG 240

[Close]

BLAST of Cucsa.049550.1 vs. NCBI nr
Match: gi|700202433|gb|KGN57566.1| (hypothetical protein Csa_3G214060 [Cucumis sativus])

HSP 1 Score: 380.6 bits (976), Expect = 1.7e-102
Identity = 173/182 (95.05%), Postives = 176/182 (96.70%), Query Frame = 1


Query: 11  ITTARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 70
           +  ARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE
Sbjct: 62  LDVARARNFPRLPAISPPIVQRWGFEETDASWITLQFPRPAVNSGIEEIPRVRITGKHLE 121

Query: 71  KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVVPWLRMHNTCPVCRYTLENIGDEGSGSG 130
           KDSNCAICKEEFEMGEEVRELPCKHFYHSDCV+PWLRMHNTCPVCRYTLENIGDEGSGSG
Sbjct: 122 KDSNCAICKEEFEMGEEVRELPCKHFYHSDCVIPWLRMHNTCPVCRYTLENIGDEGSGSG 181

Query: 131 FNGGRGEEFR-EEEEEGERRGNGWWNLVCCWWPFRVVGDWARRLRFADSGDSFFAVEPGS 190
           FNGGRGEEFR EEEEEGERRGNGWWNLVCCWWPFR+VGDWARRLRFADSGDSFFAVE   
Sbjct: 182 FNGGRGEEFREEEEEEGERRGNGWWNLVCCWWPFRLVGDWARRLRFADSGDSFFAVERKR 241

Query: 191 WM 192
           WM
Sbjct: 242 WM 243

The following BLAST results are available for this feature:

BLAST of Cucsa.049550.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RHC1A_ARATH	3.5e-23	38.30	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana GN=RHC1A PE=2...	[more]
RDUF1_ARATH	8.1e-20	43.86	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana GN=RDUF1 PE=1 SV=1	[more]
RING1_GOSHI	1.4e-19	37.10	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1	[more]
RDUF2_ARATH	1.8e-19	40.31	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana GN=DURF2 PE=1 SV=1	[more]
RNG1L_ARATH	3.1e-19	41.41	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1...	[more]

BLAST of Cucsa.049550.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LC10_CUCSA	1.2e-102	95.05	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G214060 PE=4 SV=1	[more]
A0A068TNL0_COFCA	3.9e-29	49.60	Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00014914001 PE=4 SV=1	[more]
M1C6Z4_SOLTU	1.1e-28	48.44	Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400023756 PE=4 SV=1	[more]
A0A0B2RNN4_GLYSO	7.4e-28	48.00	E3 ubiquitin-protein ligase RING1 OS=Glycine soja GN=glysoja_005509 PE=4 SV=1	[more]
K7M9N3_SOYBN	7.4e-28	48.00	Uncharacterized protein OS=Glycine max GN=GLYMA_15G047300 PE=4 SV=1	[more]

BLAST of Cucsa.049550.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G68180.1	4.0e-25	43.18	RING/U-box superfamily protein	[more]
AT2G40830.2	2.0e-24	38.30	RING-H2 finger C1A	[more]
AT3G56580.2	7.6e-24	44.07	RING/U-box superfamily protein	[more]
AT3G46620.1	4.6e-21	43.86	zinc finger (C3HC4-type RING finger) family protein	[more]
AT5G59550.1	1.0e-20	40.31	zinc finger (C3HC4-type RING finger) family protein	[more]

BLAST of Cucsa.049550.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449464484\|ref\|XP_004149959.1\|	3.2e-109	96.81	PREDICTED: E3 ubiquitin-protein ligase RNF126 isoform X1 [Cucumis sativus]	[more]
gi\|778680175\|ref\|XP_011651263.1\|	4.4e-106	95.74	PREDICTED: E3 ubiquitin-protein ligase RNF126 isoform X2 [Cucumis sativus]	[more]
gi\|659112139\|ref\|XP_008456082.1\|	9.1e-104	90.63	PREDICTED: uncharacterized protein LOC103496124 isoform X1 [Cucumis melo]	[more]
gi\|778680186\|ref\|XP_011651266.1\|	1.0e-102	96.65	PREDICTED: E3 ubiquitin-protein ligase RNF126 isoform X3 [Cucumis sativus]	[more]
gi\|700202433\|gb\|KGN57566.1\|	1.7e-102	95.05	hypothetical protein Csa_3G214060 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.049550	Cucsa.049550	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.049550.1	Cucsa.049550.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.049550.1.CDS.1	Cucsa.049550.1.CDS.1	CDS
Cucsa.049550.1.CDS.2	Cucsa.049550.1.CDS.2	CDS
Cucsa.049550.1.CDS.3	Cucsa.049550.1.CDS.3	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.049550.1.three_prime_UTR.1	Cucsa.049550.1.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 74..116 score: 6.7
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 75..115 score: 2.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 75..116 score: 12
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 70..119 score: 1.2
None	No IPR available	PANTHER	PTHR22763	RING ZINC FINGER PROTEIN	coord: 48..153 score: 6.6
None	No IPR available	PANTHER	PTHR22763:SF88	RING/U-BOX DOMAIN-CONTAINING PROTEIN	coord: 48..153 score: 6.6
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 56..120 score: 2.14