Cucsa.023000.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.023000.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Ribonuclease 3-like protein 2
Location	scaffold00320 : 277959 .. 279480 (+)
Sequence length	380

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCTATATACGGTTCAAGACTCATCAAACAACTTAGGAGGTTAGTTTATTGGATTTGAGTTATTACGTCAAAATTAATAATATAATCAATAACATTTATTGAAATTATAATAATAACACTTTATCAATAATAATCAATTAATTATATTTATAATTTACGAGTTTTAGGACATAAATTCCAACAAGATGCTGCCACGATTTCCAATGTTGGTGACCTTATGTGATCATTAAAGCAGCAAAAGGGTAATGATCCATTCAATATTTCATCCCCTCTAATGCTATACACGTAGGAAGATTAATATTGTATTACTTTCATGCATACGAGAGGTCATAGGATCCCATTTATGTTCTAGAGAATAACATACCGATAGACCTTCACTATTTTGTTCTGTTTTCTTGAGTATAGCTAATAAAAGAGTATGTCTAAGTTGCAGCCTCTTCTACTAATTTTTGAGTCCATCTTGAAAAACACTAGCAAGTAACTTCTTCATGTAAGTCACACGAGATCAATTTTTATTTCTACTCCTTCAAACGGTGGTATAATGAAATTTGCTAAGAAACAGCTTACCTGCATTGGCTGCAAGGCTCTACTAGGGTAATTTTAAGAGCTTTTCTATTCTTCATGGTTTTTGCTAGTTTGAAAGGCATATTTTCTTTTTCTTCTAACGTTCTCTGTAGCAAGTTCAGTATCTTGGTAAAGTGAATTATGTATTATTTTTTACTGCACTCCAGCAAGGCAGACAAAACTCTTATTAACATATAGTCTAATAAGATCATTACCTTCTGAATATTTTACTTGCAACCAATCTCTAATTAACATATAGTCTGATACTCTTAGCCTTATCCTTGCACTTGTTCCAAAGGATGAGCAAACGGATCATGTTATTCAGTAAGTGCTCTTGAAAAATACTTACTAATTACTTTTTtCTCATTTATCCCCCCTCCACCAAGGCCATCTCTCTTTACTTTGAGCTGCTAACATTAGCACTGAGAAGCTTGCTCGTGTTGCAGTCACCCATGGATTGTATAACTATGTTAGGTGCAATGCAGCTGCTCTCGATGATAAGGTATTCAAATTAACCTTCACTTTTCGTTTGTTCTTAAAAACATTTGATTTTTTTTGCCCTGTTTTAACTTCATGTCTTTATTTTTTGATTGTTCAGATAACTCAGTTCCTTATGGTTTGTTGATTCTATTGCCTTAGAAGGTAACTCAGTTCCTTATGGTGGGTTGATGAAAGCTCCTAAAGTTCTTGCTGACATTGTGGAATCAGTGGCTGGTGTTGTCTATGTAGATGTTAATTTTGATCTTCACAAACTATGGGTGGTGAGTTCTTAGTTACATTTGGAGTATGATTATTGTTTAGCTGGGCACCAATTAGCTCTCTTTTTGTCTTCAATGGTTTGTAGATTATTAGGGGTTTGCTAAAGCCTATCTATACTCTTGAAGAGTTACAAGTAGAGCCCCAACCTGTAACTGTACTCTTTGAAATTTTGCCAAAAAAATGGGAAGCAATATA

mRNA sequence

atgtctctatatacggttcaagactcatcaaacaacttaggagCTGCTAACATTAGCACTGAGAAGCTTGCTCGTGTTGCAGTCACCCATGGATTGTATAACTATGTTAGGTGCAATGCAGCTGCTCTCGATGATAAGATAACTCAGTTCCTTATGGTTTTTCCTTATGGTGGGTTGATGAAAGCTCCTAAAGTTCTTGCTGACATTGTGGAATCAGTGGCTGGTGTTGTCTATGTAGATGTTAATTTTGATCTTCACAAACTATGGGTGATTATTAGGGGTTTGCTAAAGCCTATCTATACTCTTGAAGAGTTACAAGTAGAGCCCCAACCTGTAACTGTACTCTTTGAAATTTTGCCAAAAAAATGGGAAGCAATATA

Coding sequence (CDS)

ATGTCTCTATATACGGTTCAAGACTCATCAAACAACTTAGGAGCTGCTAACATTAGCACTGAGAAGCTTGCTCGTGTTGCAGTCACCCATGGATTGTATAACTATGTTAGGTGCAATGCAGCTGCTCTCGATGATAAGATAACTCAGTTCCTTATGGTTTTTCCTTATGGTGGGTTGATGAAAGCTCCTAAAGTTCTTGCTGACATTGTGGAATCAGTGGCTGGTGTTGTCTATGTAGATGTTAATTTTGATCTTCACAAACTATGGGTGATTATTAGGGGTTTGCTAAAGCCTATCTATACTCTTGAAGAGTTACAAGTAGAGCCCCAACCTGTAACTGTACTCTTTGAAATTTTGCCAAAAAAATGGGAAGCAATATA

Protein sequence

MSLYTVQDSSNNLGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFLMVFPYGGLMKAPKVLADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQPVTVLFEILPKKWEAIX

Homology

[Close]

BLAST of Cucsa.023000.1 vs. Swiss-Prot
Match: RTL2_ARATH (Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana GN=RTL2 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 2.0e-32
Identity = 69/117 (58.97%), Postives = 92/117 (78.63%), Query Frame = 1


Query: 13  LGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFL--------MVFPYGGLMKAPK 72
           L AAN+STEKLARV++ HGLY+++R NA +LD+K+ +F         +   YGGL+KAPK
Sbjct: 126 LRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKEDDLSVSYGGLVKAPK 185

Query: 73  VLADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQPVTVLFEILPK 122
           VLAD+ ES+AG VYVDVNFDL +LWVI RGLL+PI TL++LQ +PQPV++LF++  K
Sbjct: 186 VLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQPVSMLFKLCHK 242

[Close]

BLAST of Cucsa.023000.1 vs. Swiss-Prot
Match: RTL2_ORYSJ (Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica GN=Os05g0271300 PE=2 SV=2)

HSP 1 Score: 107.1 bits (266), Expect = 1.5e-22
Identity = 64/123 (52.03%), Postives = 77/123 (62.60%), Query Frame = 1


Query: 11  NNLGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQF-----------LMVFPYGG- 70
           + L AANISTEKLARVAV H LY  +R N   LD  + QF           L   PYGG 
Sbjct: 102 STLRAANISTEKLARVAVRHDLYPLLRRNCPRLDLLVGQFIETVKQEPEDDLSTVPYGGS 161

Query: 71  LMKAPKVLADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQPVTVLFEI 122
           ++KAPKVLADIVE++A  VYVD  FDL KLW + R L +PI T E   ++ QPVT+L E+
Sbjct: 162 VVKAPKVLADIVEAIAAAVYVDCKFDLEKLWKVTRWLFEPIITAE--TIDEQPVTMLHEL 221

[Close]

BLAST of Cucsa.023000.1 vs. Swiss-Prot
Match: RTL3_ARATH (Ribonuclease 3-like protein 3 OS=Arabidopsis thaliana GN=RTL3 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 4.7e-21
Identity = 51/118 (43.22%), Postives = 76/118 (64.41%), Query Frame = 1


Query: 12  NLGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFLMVF-------PYGGLMKAPK 71
           +L  AN+S EK AR+AV H L++++     +L  K+  F           PYGGL+KAPK
Sbjct: 65  DLRTANVSNEKFARIAVNHNLHHFLLLQNPSLFKKVKNFAEAVRKEDDPVPYGGLVKAPK 124

Query: 72  VLADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQ-PVTVLFEILPK 122
           +LAD +ES+A  V++DVN+D+ +LW I R LL+PIYT ++L ++P+ P   LF +  K
Sbjct: 125 ILADTLESIAATVFIDVNYDVKRLWEIFRSLLEPIYTPDDLLLQPKLPFLTLFRLADK 182

HSP 2 Score: 68.6 bits (166), Expect = 5.8e-11
Identity = 42/117 (35.90%), Postives = 68/117 (58.12%), Query Frame = 1


Query: 16  ANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFLMVF-----PYGGLMKAPKVLADIV 75
           AN   +K A VAV  G+Y     +    +   T F+ +      PY  ++KAPK+L +++
Sbjct: 481 ANTCHDKYACVAVKKGIYQSFIGSVPKPEKMTTDFIELMGKEDDPYR-VVKAPKILVNLL 540

Query: 76  ESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVE-PQPVTVLFEILPKKWEAI 127
             VAG VY+DV +++ +L  I R LL+PIYTL++L+++  QP  +LF +  K  + I
Sbjct: 541 AGVAGAVYIDVKYNVQRLLEIFRVLLEPIYTLDDLRMQLKQPFLMLFRLADKHGKQI 596

[Close]

BLAST of Cucsa.023000.1 vs. Swiss-Prot
Match: RTL3_ORYSJ (Ribonuclease 3-like protein 3 OS=Oryza sativa subsp. japonica GN=Os06g0358800 PE=2 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 5.4e-17
Identity = 51/114 (44.74%), Postives = 70/114 (61.40%), Query Frame = 1


Query: 13  LGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQF---LMVFPY--GGLMKAPKVLA 72
           L AAN+  EKLARVAV HGL++++R  A  LD +IT F   L ++P    GL+  PKVL 
Sbjct: 109 LRAANVDKEKLARVAVVHGLHHFLRHKAPNLDGQITDFIEELSMYPIHSNGLLDPPKVLC 168

Query: 73  DIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQPVTVLFEILPK 122
           D+VES+ G +Y D NF+   +W + + L  P+ +LE L     PV+ LFE   K
Sbjct: 169 DVVESLIGAIYCDSNFNQEIVWQVFQKLADPLISLETL--GKHPVSELFEFCQK 220

[Close]

BLAST of Cucsa.023000.1 vs. Swiss-Prot
Match: DCL3A_ORYSJ (Endoribonuclease Dicer homolog 3a OS=Oryza sativa subsp. japonica GN=DCL3A PE=2 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 3.1e-12
Identity = 40/115 (34.78%), Postives = 65/115 (56.52%), Query Frame = 1


Query: 5    TVQDSSNNLGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFL----------MVF 64
            T +    +L +A+++ E  A+VAV H  +++++ ++  L D+IT+++          +  
Sbjct: 1302 TNEGELTDLRSASVNNENFAQVAVKHNFHHFLQHSSGLLLDQITEYVNRLEGSSMDKVEL 1361

Query: 65   PYGGLMKAPKVLADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEP 110
               GL K PKVL DIVES+AG + +D   DL  +W I   LL PI T E L++ P
Sbjct: 1362 LSDGLPKGPKVLGDIVESIAGAILLDTKLDLDVVWGIFEPLLSPIVTPENLELPP 1416

[Close]

BLAST of Cucsa.023000.1 vs. TrEMBL
Match: A0A0A0L1E2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G192230 PE=4 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 1.6e-39
Identity = 86/121 (71.07%), Postives = 100/121 (82.64%), Query Frame = 1


Query: 13  LGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFL-------MVFPYGGLMKAPKV 72
           L AAN+STEKLARVAVTHGLYNYVR ++ A+DDK+ +F+          PYGG+MKAPKV
Sbjct: 71  LRAANVSTEKLARVAVTHGLYNYVRRDSPAMDDKVKEFVDSVALEGSSVPYGGMMKAPKV 130

Query: 73  LADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQPVTVLFEILPKKWEA 127
           LADIVESVAG VYVDVNFDL KLWVIIRGLL+PIYTLEELQVEPQPVTVLF++  +  + 
Sbjct: 131 LADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFQVCQRNGKQ 190

[Close]

BLAST of Cucsa.023000.1 vs. TrEMBL
Match: A0A061F5X1_THECC (Ribonuclease 3-like protein 2 OS=Theobroma cacao GN=TCM_030826 PE=4 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 8.4e-33
Identity = 78/115 (67.83%), Postives = 89/115 (77.39%), Query Frame = 1


Query: 15  AANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFLMVFP-------YGGLMKAPKVLA 74
           AANISTEKLARVA+ H L+ +VR NA +L+DK+ +F  V         YGG MKAPKVLA
Sbjct: 115 AANISTEKLARVAIKHRLFQFVRHNATSLEDKVREFAKVVSQEDDPVSYGGSMKAPKVLA 174

Query: 75  DIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQPVTVLFEILPKK 123
           DIVESVA  VYVDVNFDL KLWVIIRGLL+PI TLE+LQ +PQPVTVLFE   K+
Sbjct: 175 DIVESVAAAVYVDVNFDLSKLWVIIRGLLEPIVTLEDLQQQPQPVTVLFEFCQKQ 229

[Close]

BLAST of Cucsa.023000.1 vs. TrEMBL
Match: A0A067KSH7_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_00928 PE=4 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 4.1e-32
Identity = 73/116 (62.93%), Postives = 89/116 (76.72%), Query Frame = 1


Query: 13  LGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFLMVF-------PYGGLMKAPKV 72
           L AANISTEKLARVA+ HGLY +VR NAA+LDDK+T+F            +GG +KAPK+
Sbjct: 110 LRAANISTEKLARVAIRHGLYRFVRRNAASLDDKVTEFTDAIIHEDDPVAHGGSVKAPKI 169

Query: 73  LADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQPVTVLFEILPK 122
           LADI+ES+A  +Y DVNFDL +LWVI RGLL+PI TLE+LQ +PQPVT LFE+  K
Sbjct: 170 LADIIESIAAAIYFDVNFDLQRLWVIFRGLLEPIVTLEDLQQQPQPVTKLFELCQK 225

[Close]

BLAST of Cucsa.023000.1 vs. TrEMBL
Match: V4TYN2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10008694mg PE=4 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 7.1e-32
Identity = 74/121 (61.16%), Postives = 91/121 (75.21%), Query Frame = 1


Query: 13  LGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFLMVFP-------YGGLMKAPKV 72
           L AANISTEKLARVAV HG Y +VR +AAALDDK+ +F            YGG +KAPKV
Sbjct: 102 LRAANISTEKLARVAVKHGFYKFVRHSAAALDDKVREFAEAVSQEDNTAVYGGSIKAPKV 161

Query: 73  LADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQPVTVLFEILPKKWEA 127
           LADIVES+A  +YVD++FDL KLW+I RGLL+PI TLEELQ +PQPVT+LF++  K  + 
Sbjct: 162 LADIVESIAAAIYVDIDFDLQKLWMIFRGLLEPIVTLEELQQQPQPVTLLFKLCQKNGKQ 221

[Close]

BLAST of Cucsa.023000.1 vs. TrEMBL
Match: A0A067F7H9_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g017515mg PE=4 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 7.1e-32
Identity = 74/121 (61.16%), Postives = 91/121 (75.21%), Query Frame = 1


Query: 13  LGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFLMVFP-------YGGLMKAPKV 72
           L AANISTEKLARVAV HG Y +VR +AAALDDK+ +F            YGG +KAPKV
Sbjct: 102 LRAANISTEKLARVAVKHGFYKFVRHSAAALDDKVKEFAEAVSQEDNTAVYGGSIKAPKV 161

Query: 73  LADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQPVTVLFEILPKKWEA 127
           LADIVES+A  +YVD++FDL KLW+I RGLL+PI TLEELQ +PQPVT+LF++  K  + 
Sbjct: 162 LADIVESIAAAIYVDIDFDLQKLWMIFRGLLEPIVTLEELQQQPQPVTLLFKLCQKNGKQ 221

[Close]

BLAST of Cucsa.023000.1 vs. TAIR10
Match: AT3G20420.1 (AT3G20420.1 RNAse THREE-like protein 2)

HSP 1 Score: 139.8 bits (351), Expect = 1.2e-33
Identity = 69/117 (58.97%), Postives = 92/117 (78.63%), Query Frame = 1


Query: 13  LGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFL--------MVFPYGGLMKAPK 72
           L AAN+STEKLARV++ HGLY+++R NA +LD+K+ +F         +   YGGL+KAPK
Sbjct: 126 LRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKEDDLSVSYGGLVKAPK 185

Query: 73  VLADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQPVTVLFEILPK 122
           VLAD+ ES+AG VYVDVNFDL +LWVI RGLL+PI TL++LQ +PQPV++LF++  K
Sbjct: 186 VLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQPVSMLFKLCHK 242

[Close]

BLAST of Cucsa.023000.1 vs. TAIR10
Match: AT5G45150.1 (AT5G45150.1 RNAse THREE-like protein 3)

HSP 1 Score: 102.1 bits (253), Expect = 2.7e-22
Identity = 51/118 (43.22%), Postives = 76/118 (64.41%), Query Frame = 1


Query: 12  NLGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFLMVF-------PYGGLMKAPK 71
           +L  AN+S EK AR+AV H L++++     +L  K+  F           PYGGL+KAPK
Sbjct: 65  DLRTANVSNEKFARIAVNHNLHHFLLLQNPSLFKKVKNFAEAVRKEDDPVPYGGLVKAPK 124

Query: 72  VLADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQ-PVTVLFEILPK 122
           +LAD +ES+A  V++DVN+D+ +LW I R LL+PIYT ++L ++P+ P   LF +  K
Sbjct: 125 ILADTLESIAATVFIDVNYDVKRLWEIFRSLLEPIYTPDDLLLQPKLPFLTLFRLADK 182

HSP 2 Score: 68.6 bits (166), Expect = 3.3e-12
Identity = 42/117 (35.90%), Postives = 68/117 (58.12%), Query Frame = 1


Query: 16  ANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFLMVF-----PYGGLMKAPKVLADIV 75
           AN   +K A VAV  G+Y     +    +   T F+ +      PY  ++KAPK+L +++
Sbjct: 481 ANTCHDKYACVAVKKGIYQSFIGSVPKPEKMTTDFIELMGKEDDPYR-VVKAPKILVNLL 540

Query: 76  ESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVE-PQPVTVLFEILPKKWEAI 127
             VAG VY+DV +++ +L  I R LL+PIYTL++L+++  QP  +LF +  K  + I
Sbjct: 541 AGVAGAVYIDVKYNVQRLLEIFRVLLEPIYTLDDLRMQLKQPFLMLFRLADKHGKQI 596

[Close]

BLAST of Cucsa.023000.1 vs. TAIR10
Match: AT4G15417.1 (AT4G15417.1 RNAse II-like 1)

HSP 1 Score: 84.3 bits (207), Expect = 5.7e-17
Identity = 49/115 (42.61%), Postives = 73/115 (63.48%), Query Frame = 1


Query: 13  LGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFLMV---FPY--GGLMKAPKVLA 72
           L A N+ TEKLARVAV H LY+Y+R     L+++I +F+     +P    GL+K PKVLA
Sbjct: 97  LRAVNVDTEKLARVAVNHQLYSYLRHKKPLLEEQILEFVEAMEKYPLHSNGLLKVPKVLA 156

Query: 73  DIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQPVTVLFEILPKK 123
           DIVES  G +++D N     +W +I+ LL+PI  L+++  +  P+T L E+  K+
Sbjct: 157 DIVESTIGAIFMDCN-STETVWKVIKPLLEPIIHLDKM--KSHPMTELNEMCQKR 208

[Close]

BLAST of Cucsa.023000.1 vs. TAIR10
Match: AT1G01040.2 (AT1G01040.2 dicer-like 1)

HSP 1 Score: 70.5 bits (171), Expect = 8.6e-13
Identity = 42/109 (38.53%), Postives = 63/109 (57.80%), Query Frame = 1


Query: 12   NLGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFLMVFP----------YG-GLM 71
            +L AA ++ E  ARVAV H L+ Y+R  ++AL+ +I +F+              +G G  
Sbjct: 1627 DLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNSFGLGDC 1686

Query: 72   KAPKVLADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEP 110
            KAPKVL DIVES+AG +++D   D    W + + LL+P+ T E L + P
Sbjct: 1687 KAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPMHP 1735

[Close]

BLAST of Cucsa.023000.1 vs. TAIR10
Match: AT3G43920.2 (AT3G43920.2 dicer-like 3)

HSP 1 Score: 65.5 bits (158), Expect = 2.8e-11
Identity = 34/105 (32.38%), Postives = 60/105 (57.14%), Query Frame = 1


Query: 12   NLGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFLMVFPYGG-------LMKAPK 71
            +L +A ++ E  A+VAV + L+ +++  A  L+ +I  +LM F            ++ PK
Sbjct: 1263 DLRSACVNNENFAQVAVKNNLHTHLQRCATVLETQINDYLMSFQKPDETGRSIPSIQGPK 1322

Query: 72   VLADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEP 110
             L D+VES+AG + +D   DL ++W +   LL P+ T ++LQ+ P
Sbjct: 1323 ALGDVVESIAGALLIDTRLDLDQVWRVFEPLLSPLVTPDKLQLPP 1367

[Close]

BLAST of Cucsa.023000.1 vs. NCBI nr
Match: gi|659096529|ref|XP_008449146.1| (PREDICTED: ribonuclease 3-like protein 2 [Cucumis melo])

HSP 1 Score: 171.4 bits (433), Expect = 1.0e-39
Identity = 88/121 (72.73%), Postives = 99/121 (81.82%), Query Frame = 1


Query: 13  LGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFL-------MVFPYGGLMKAPKV 72
           L AAN+STEKLARVAVTHGLYNYVR NAA +D K+ +F+          PYGGLMKAPKV
Sbjct: 71  LRAANVSTEKLARVAVTHGLYNYVRRNAATMDAKVKEFVDSVALEGSSVPYGGLMKAPKV 130

Query: 73  LADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQPVTVLFEILPKKWEA 127
           LADIVESVAG VYVDVNFDL KLWVIIRGLL+PIYTLEELQVEPQPVTVLF++  +  + 
Sbjct: 131 LADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFDVCQRNGKQ 190

[Close]

BLAST of Cucsa.023000.1 vs. NCBI nr
Match: gi|778697607|ref|XP_011654357.1| (PREDICTED: ribonuclease 3-like protein 2 [Cucumis sativus])

HSP 1 Score: 170.2 bits (430), Expect = 2.3e-39
Identity = 86/121 (71.07%), Postives = 100/121 (82.64%), Query Frame = 1


Query: 13  LGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFL-------MVFPYGGLMKAPKV 72
           L AAN+STEKLARVAVTHGLYNYVR ++ A+DDK+ +F+          PYGG+MKAPKV
Sbjct: 71  LRAANVSTEKLARVAVTHGLYNYVRRDSPAMDDKVKEFVDSVALEGSSVPYGGMMKAPKV 130

Query: 73  LADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQPVTVLFEILPKKWEA 127
           LADIVESVAG VYVDVNFDL KLWVIIRGLL+PIYTLEELQVEPQPVTVLF++  +  + 
Sbjct: 131 LADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFQVCQRNGKQ 190

[Close]

BLAST of Cucsa.023000.1 vs. NCBI nr
Match: gi|778697607|ref|XP_011654357.1| (PREDICTED: ribonuclease 3-like protein 2 [Cucumis sativus])

HSP 1 Score: 142.5 bits (358), Expect = 5.0e-31
Identity = 75/121 (61.98%), Postives = 88/121 (72.73%), Query Frame = 1


Query: 13  LGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFLMV-------FPYGGLMKAPKV 72
           L AAN+STEKLARVAV H LY+YVR NA  LDD + +F           PY GL+KAPKV
Sbjct: 446 LRAANVSTEKLARVAVLHRLYSYVRRNADDLDDNVREFADAVALEDNSIPYVGLVKAPKV 505

Query: 73  LADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQPVTVLFEILPKKWEA 127
           LADIVESVAG +YVDV FDL KLW II+  LKPIYT E+L+ +PQPVT+LFE   KK + 
Sbjct: 506 LADIVESVAGAIYVDVEFDLQKLWAIIKDFLKPIYTPEDLEADPQPVTLLFEDCQKKGKQ 565

HSP 2 Score: 170.2 bits (430), Expect = 2.3e-39
Identity = 86/121 (71.07%), Postives = 100/121 (82.64%), Query Frame = 1


Query: 13  LGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFL-------MVFPYGGLMKAPKV 72
           L AAN+STEKLARVAVTHGLYNYVR ++ A+DDK+ +F+          PYGG+MKAPKV
Sbjct: 71  LRAANVSTEKLARVAVTHGLYNYVRRDSPAMDDKVKEFVDSVALEGSSVPYGGMMKAPKV 130

Query: 73  LADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQPVTVLFEILPKKWEA 127
           LADIVESVAG VYVDVNFDL KLWVIIRGLL+PIYTLEELQVEPQPVTVLF++  +  + 
Sbjct: 131 LADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVLFQVCQRNGKQ 190

[Close]

BLAST of Cucsa.023000.1 vs. NCBI nr
Match: gi|659096535|ref|XP_008449149.1| (PREDICTED: ribonuclease 3-like protein 2 isoform X3 [Cucumis melo])

HSP 1 Score: 151.8 bits (382), Expect = 8.3e-34
Identity = 78/121 (64.46%), Postives = 91/121 (75.21%), Query Frame = 1


Query: 13  LGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFLMV-------FPYGGLMKAPKV 72
           L AAN+STEKLARVAV HGLY+YVR +AA LDD + +F           PYGGL KAPKV
Sbjct: 67  LRAANVSTEKLARVAVLHGLYDYVRRSAADLDDNVREFADAVALEDNSIPYGGLKKAPKV 126

Query: 73  LADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQPVTVLFEILPKKWEA 127
           LADIVESVAG +YVDV FDL KLW II+ LLKPIYT E+L+ EPQPVT+LFE+  K  + 
Sbjct: 127 LADIVESVAGAIYVDVEFDLQKLWAIIKDLLKPIYTPEDLEAEPQPVTLLFEVCQKNGKQ 186

[Close]

BLAST of Cucsa.023000.1 vs. NCBI nr
Match: gi|659096533|ref|XP_008449148.1| (PREDICTED: ribonuclease 3-like protein 2 isoform X2 [Cucumis melo])

HSP 1 Score: 151.8 bits (382), Expect = 8.3e-34
Identity = 78/121 (64.46%), Postives = 91/121 (75.21%), Query Frame = 1


Query: 13  LGAANISTEKLARVAVTHGLYNYVRCNAAALDDKITQFLMV-------FPYGGLMKAPKV 72
           L AAN+STEKLARVAV HGLY+YVR +AA LDD + +F           PYGGL KAPKV
Sbjct: 67  LRAANVSTEKLARVAVLHGLYDYVRRSAADLDDNVREFADAVALEDNSIPYGGLKKAPKV 126

Query: 73  LADIVESVAGVVYVDVNFDLHKLWVIIRGLLKPIYTLEELQVEPQPVTVLFEILPKKWEA 127
           LADIVESVAG +YVDV FDL KLW II+ LLKPIYT E+L+ EPQPVT+LFE+  K  + 
Sbjct: 127 LADIVESVAGAIYVDVEFDLQKLWAIIKDLLKPIYTPEDLEAEPQPVTLLFEVCQKNGKQ 186

The following BLAST results are available for this feature:

BLAST of Cucsa.023000.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RTL2_ARATH	2.0e-32	58.97	Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana GN=RTL2 PE=1 SV=1	[more]
RTL2_ORYSJ	1.5e-22	52.03	Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica GN=Os05g0271300 PE...	[more]
RTL3_ARATH	4.7e-21	43.22	Ribonuclease 3-like protein 3 OS=Arabidopsis thaliana GN=RTL3 PE=2 SV=1	[more]
RTL3_ORYSJ	5.4e-17	44.74	Ribonuclease 3-like protein 3 OS=Oryza sativa subsp. japonica GN=Os06g0358800 PE...	[more]
DCL3A_ORYSJ	3.1e-12	34.78	Endoribonuclease Dicer homolog 3a OS=Oryza sativa subsp. japonica GN=DCL3A PE=2 ...	[more]

BLAST of Cucsa.023000.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1E2_CUCSA	1.6e-39	71.07	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G192230 PE=4 SV=1	[more]
A0A061F5X1_THECC	8.4e-33	67.83	Ribonuclease 3-like protein 2 OS=Theobroma cacao GN=TCM_030826 PE=4 SV=1	[more]
A0A067KSH7_JATCU	4.1e-32	62.93	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_00928 PE=4 SV=1	[more]
V4TYN2_9ROSI	7.1e-32	61.16	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10008694mg PE=4 SV=1	[more]
A0A067F7H9_CITSI	7.1e-32	61.16	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g017515mg PE=4 SV=1	[more]

BLAST of Cucsa.023000.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G20420.1	1.2e-33	58.97	RNAse THREE-like protein 2	[more]
AT5G45150.1	2.7e-22	43.22	RNAse THREE-like protein 3	[more]
AT4G15417.1	5.7e-17	42.61	RNAse II-like 1	[more]
AT1G01040.2	8.6e-13	38.53	dicer-like 1	[more]
AT3G43920.2	2.8e-11	32.38	dicer-like 3	[more]

BLAST of Cucsa.023000.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659096529\|ref\|XP_008449146.1\|	1.0e-39	72.73	PREDICTED: ribonuclease 3-like protein 2 [Cucumis melo]	[more]
gi\|778697607\|ref\|XP_011654357.1\|	2.3e-39	71.07	PREDICTED: ribonuclease 3-like protein 2 [Cucumis sativus]	[more]
gi\|778697607\|ref\|XP_011654357.1\|	5.0e-31	61.98	PREDICTED: ribonuclease 3-like protein 2 [Cucumis sativus]	[more]
gi\|659096535\|ref\|XP_008449149.1\|	8.3e-34	64.46	PREDICTED: ribonuclease 3-like protein 2 isoform X3 [Cucumis melo]	[more]
gi\|659096533\|ref\|XP_008449148.1\|	8.3e-34	64.46	PREDICTED: ribonuclease 3-like protein 2 isoform X2 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR000999	RNase_III_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004525	ribonuclease III activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006396	RNA processing

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006396	RNA processing
biological_process	GO:0051252	regulation of RNA metabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004525	ribonuclease III activity
molecular_function	GO:0003723	RNA binding

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.023000	Cucsa.023000	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.023000.1	Cucsa.023000.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.023000.1.CDS.1	Cucsa.023000.1.CDS.1	CDS
Cucsa.023000.1.CDS.2	Cucsa.023000.1.CDS.2	CDS
Cucsa.023000.1.CDS.3	Cucsa.023000.1.CDS.3	CDS
Cucsa.023000.1.CDS.4	Cucsa.023000.1.CDS.4	CDS
Cucsa.023000.1.CDS.5	Cucsa.023000.1.CDS.5	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000999	Ribonuclease III domain	GENE3D	G3DSA:1.10.1520.10		coord: 13..101 score: 1.8
IPR000999	Ribonuclease III domain	PFAM	PF00636	Ribonuclease_3	coord: 13..80 score: 4.
IPR000999	Ribonuclease III domain	PROFILE	PS50142	RNASE_3_2	coord: 11..82 score: 11
IPR000999	Ribonuclease III domain	unknown	SSF69065	RNase III domain-like	coord: 15..101 score: 8.37
None	No IPR available	PANTHER	PTHR14950	HELICASE-RELATED	coord: 13..118 score: 1.2
None	No IPR available	PANTHER	PTHR14950:SF22	SUBFAMILY NOT NAMED	coord: 13..118 score: 1.2