Cucsa.302200 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.302200
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Cytochrome P450 family protein
Location	scaffold02951 : 409506 .. 411759 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCCGCTTGCAGTTGCCACCAGGTCCTCGCGGTGTTCCGCTGCTTGGAAACCTGCCTTTTCTAGACCCAAATCTTCACACTTACTTCATGGAGTTAGGCCAAAAATACGGCTCAATTGTAAAGCTCCAACTCGGTGGAAAGGTTGGTATAATCGTGAATTCACCATCAGTGGCACGTGAAATATTAAAGGACCATGATATCACGTTTGCTAATCGTGATGTTCCTCAAGCGGGCCGAGTTGCCACATATGGCGGTTTTGACATAACTTGGACCCCATACGGACCTGAGTGGCGTATGTTGAGAAAAGTTTGTACAATTAAGTTGCTTGGCAATGCTAGTTTGGACATGGTTTATGAGCTCCGTCGTAGTGAGGTTAGGAAAACAGTGGCTCAGTTGTATCAGAGAGCTGAGTCGACGGTAAAGATAGGAGAGCAGGTTTTTTTtACAGTTTTCAACGTGATTACGAGTATGTTGTGGGGTGGGACAATGGAAGGGGAGGAAAAGGCTGCCGTTGCAGTTGTGTTTCGAGAGATGGTTTCAGCCATGACTGAGTTAGCGGGTAAACCTAATATTTCTGATTTTTTtCCAAGCTTGGCTTGTTTGGATGTTCAAGGAATTGAGAAGAAAATGCTTAAGCTTCTCCCTAAATTGGACACTATTTTTGAAAAGTTGATTGATGAACGATTGAGAATGACGAATAATGAAGAAGGGAGTAGAAATAAAAATGATTTCTtGCAGTTTTTATTAAAAGTCAAAGATGAAAGCGATTCTCAAACACCTCTCACAGTGGTTCAACTCAAAGCCTTGCTAATGGTATGTATTTTCACCTTCCATCATCTAGAAATTTTTAACTTTttTTTttGTATATATATATTATAGTATTCCATCATCTACATTTACTTTAACCACGAAAAATATTCAACAGCCGATTGGATTGTTCTTATTTTGTTTAATATTATATTGTTAACGAAAATTGTAATATGAAAaTAAGCTATGCCTAATCATTAATTAAACAACCTTCTTTACTTGATATAATATATGAATAATTAGTGTTTGTCTTCGAAAAAAAaTCAGTAACTATTGATTGAATCAATTATTTTATGAAAAAAaGTCCAAATTATAGATGTGATGGAGAAGTTAGCAATTACTTTGGCCGGTTAGTATCTTTATTGTTATGGTCCATCTATATTTATTGTTACGGTCCATATATATTTTCTTATATCAACTTCTACTTTGTCCATCCTTTCATAATCATTTGCTTTTTAAGTTTTAATTTTAGAAAATTAAATTTCACCCATCTTGTTCCTTTTtAATCTTTTTAAGTGTATATATTTCGTAAATGTTCTCTTTATTACAATTTCTATTCTTAGAAAaCAAAaTAAATTTAATTTTGGAAAGCAAAAACAAGAAGATGAAAAAAaCGAAAAGATTTCTGAAAAACATTCAACTTTTCAAAATGGTTGATTCATAGTAGAGGAGAACTGCCACATTCTTGTAAACATATTTGAATCGACTCTAAAGAAGGGGACAATGTTTTTGGAGCAGGATATGGTGTTTGGTGGAACAGACACATCCTCAAACACCATTGAATTCGCAATGGCGGAGATGATGAAGAACCCAAAAGTTGCAGAGAAAGCAAAGGAAGAACTCCGTGCCGTGGTTGGAGAACAGAGTATTGTTGAAGAATCCCATATACAGAGTTTGCCATATCTCAAAGCCATAATGAAAGAAACATTGCGTCTTCATCCAATTCTGCCGCTGCTAGTGCCGCATTGCCCCAGCGACACCACCGTCGTCTCCAATTACACAATTCCAAAGGGTTCTCGAGTGTTCGTCAACGTCTGGGCCATTCAACGAGACCCCAATGAATGGGAGAATCCATTGGAGTTCGACCCAGAGAGGTTTATGAACGGTAAATTGGATTTCAGTGGGAGTGATTTTCGTTATTTCCCATTTGGATCTGGTAGAAGAAAATGTCCTGGGATTGCCATGGGGGAAAGAATGGTAATGTATTTGCTTGCTACGCTTTTGCATTCGTTCGATTGGAAATTGGAAGAAGGTGAAGAGATTGAAATTGAGGAGAAaTTTGGGATTGTTTTAACGATGAAGAAGCCTCTTGTTCTCATCCCCACGCCTAGATTATCAGACCCTACTCTGTATCAGTGAACAAATTGGCTTTTCTTCCGCCATTGTTGTTCCTAATTCAAAACTTCTCAAATTTCAAATAAAATCGTGAGCTTCAGCGTGTTGGGTT

mRNA sequence

CCCCGCTTGCAGTTGCCACCAGGTCCTCGCGGTGTTCCGCTGCTTGGAAACCTGCCTTTTCTAGACCCAAATCTTCACACTTACTTCATGGAGTTAGGCCAAAAATACGGCTCAATTGTAAAGCTCCAACTCGGTGGAAAGGTTGGTATAATCGTGAATTCACCATCAGTGGCACGTGAAATATTAAAGGACCATGATATCACGTTTGCTAATCGTGATGTTCCTCAAGCGGGCCGAGTTGCCACATATGGCGGTTTTGACATAACTTGGACCCCATACGGACCTGAGTGGCGTATGTTGAGAAAAGTTTGTACAATTAAGTTGCTTGGCAATGCTAGTTTGGACATGGTTTATGAGCTCCGTCGTAGTGAGGTTAGGAAAACAGTGGCTCAGTTGTATCAGAGAGCTGAGTCGACGGTAAAGATAGGAGAGCAGGTTTTTTTTACAGTTTTCAACGTGATTACGAGTATGTTGTGGGGTGGGACAATGGAAGGGGAGGAAAAGGCTGCCGTTGCAGTTGTGTTTCGAGAGATGGTTTCAGCCATGACTGAGTTAGCGGGTAAACCTAATATTTCTGATTTTTTTCCAAGCTTGGCTTGTTTGGATGTTCAAGGAATTGAGAAGAAAATGCTTAAGCTTCTCCCTAAATTGGACACTATTTTTGAAAAGTTGATTGATGAACGATTGAGAATGACGAATAATGAAGAAGGGAGTAGAAATAAAAATGATTTCTTGCAGTTTTTATTAAAAGTCAAAGATGAAAGCGATTCTCAAACACCTCTCACAGTGGTTCAACTCAAAGCCTTGCTAATGGATATGGTGTTTGGTGGAACAGACACATCCTCAAACACCATTGAATTCGCAATGGCGGAGATGATGAAGAACCCAAAAGTTGCAGAGAAAGCAAAGGAAGAACTCCGTGCCGTGGTTGGAGAACAGAGTATTGTTGAAGAATCCCATATACAGAGTTTGCCATATCTCAAAGCCATAATGAAAGAAACATTGCGTCTTCATCCAATTCTGCCGCTGCTAGTGCCGCATTGCCCCAGCGACACCACCGTCGTCTCCAATTACACAATTCCAAAGGGTTCTCGAGTGTTCGTCAACGTCTGGGCCATTCAACGAGACCCCAATGAATGGGAGAATCCATTGGAGTTCGACCCAGAGAGGTTTATGAACGGTAAATTGGATTTCAGTGGGAGTGATTTTCGTTATTTCCCATTTGGATCTGGTAGAAGAAAATGTCCTGGGATTGCCATGGGGGAAAGAATGGTAATGTATTTGCTTGCTACGCTTTTGCATTCGTTCGATTGGAAATTGGAAGAAGGTGAAGAGATTGAAATTGAGGAGAAATTTGGGATTGTTTTAACGATGAAGAAGCCTCTTGTTCTCATCCCCACGCCTAGATTATCAGACCCTACTCTGTATCAGTGAACAAATTGGCTTTTCTTCCGCCATTGTTGTTCCTAATTCAAAACTTCTCAAATTTCAAATAAAATCGTGAGCTTCAgcgtgttgggtt

Coding sequence (CDS)

ATGGAGTTAGGCCAAAAATACGGCTCAATTGTAAAGCTCCAACTCGGTGGAAAGGTTGGTATAATCGTGAATTCACCATCAGTGGCACGTGAAATATTAAAGGACCATGATATCACGTTTGCTAATCGTGATGTTCCTCAAGCGGGCCGAGTTGCCACATATGGCGGTTTTGACATAACTTGGACCCCATACGGACCTGAGTGGCGTATGTTGAGAAAAGTTTGTACAATTAAGTTGCTTGGCAATGCTAGTTTGGACATGGTTTATGAGCTCCGTCGTAGTGAGGTTAGGAAAACAGTGGCTCAGTTGTATCAGAGAGCTGAGTCGACGGTAAAGATAGGAGAGCAGGTTTTTTTtACAGTTTTCAACGTGATTACGAGTATGTTGTGGGGTGGGACAATGGAAGGGGAGGAAAAGGCTGCCGTTGCAGTTGTGTTTCGAGAGATGGTTTCAGCCATGACTGAGTTAGCGGGTAAACCTAATATTTCTGATTTTTTtCCAAGCTTGGCTTGTTTGGATGTTCAAGGAATTGAGAAGAAAATGCTTAAGCTTCTCCCTAAATTGGACACTATTTTTGAAAAGTTGATTGATGAACGATTGAGAATGACGAATAATGAAGAAGGGAGTAGAAATAAAAATGATTTCTtGCAGTTTTTATTAAAAGTCAAAGATGAAAGCGATTCTCAAACACCTCTCACAGTGGTTCAACTCAAAGCCTTGCTAATGGATATGGTGTTTGGTGGAACAGACACATCCTCAAACACCATTGAATTCGCAATGGCGGAGATGATGAAGAACCCAAAAGTTGCAGAGAAAGCAAAGGAAGAACTCCGTGCCGTGGTTGGAGAACAGAGTATTGTTGAAGAATCCCATATACAGAGTTTGCCATATCTCAAAGCCATAATGAAAGAAACATTGCGTCTTCATCCAATTCTGCCGCTGCTAGTGCCGCATTGCCCCAGCGACACCACCGTCGTCTCCAATTACACAATTCCAAAGGGTTCTCGAGTGTTCGTCAACGTCTGGGCCATTCAACGAGACCCCAATGAATGGGAGAATCCATTGGAGTTCGACCCAGAGAGGTTTATGAACGGTAAATTGGATTTCAGTGGGAGTGATTTTCGTTATTTCCCATTTGGATCTGGTAGAAGAAAATGTCCTGGGATTGCCATGGGGGAAAGAATGGTAATGTATTTGCTTGCTACGCTTTTGCATTCGTTCGATTGGAAATTGGAAGAAGGTGAAGAGATTGAAATTGAGGAGAAaTTTGGGATTGTTTTAACGATGAAGAAGCCTCTTGTTCTCATCCCCACGCCTAGATTATCAGACCCTACTCTGTATCAGTGA

Protein sequence

MELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQVFFTVFNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEKKMLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQLKALLMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYLKAIMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFDPERFMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEEIEIEEKFGIVLTMKKPLVLIPTPRLSDPTLYQ*

Homology

[Close]

BLAST of Cucsa.302200 vs. Swiss-Prot
Match: C75A2_SOLME (Flavonoid 3',5'-hydroxylase OS=Solanum melongena GN=CYP75A2 PE=2 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 2.9e-89
Identity = 180/448 (40.18%), Postives = 270/448 (60.27%), Query Frame = 1


Query: 2   ELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRV-ATYGGFDIT 61
           ++ +KYG I+ L++G    ++ ++P+ A+  LK  DI F+NR  P AG     Y   D+ 
Sbjct: 62  KMAKKYGPIMYLKVGTCGMVVASTPNAAKAFLKTLDINFSNRP-PNAGATHMAYNAQDMV 121

Query: 62  WTPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAE--STVKIGEQVF 121
           + PYGP W++LRK+  + +LG  +L+    +R +E+   +  ++  +     + + + + 
Sbjct: 122 FAPYGPRWKLLRKLSNLHMLGGKALENWANVRANELGHMLKSMFDASHVGERIVVADMLT 181

Query: 122 FTVFNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIE 181
           F + N+I  ++    +   EK      F+ MV  +  +AG  NI DF P +A +D+QGIE
Sbjct: 182 FAMANMIGQVMLSKRVF-VEKGKEVNEFKNMVVELMTVAGYFNIGDFIPQIAWMDLQGIE 241

Query: 182 KKMLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQLK 241
           K M KL  K D +  K+ +E    +N  +G   K DFL F++  +D S+ +  L++  +K
Sbjct: 242 KGMKKLHKKFDDLLTKMFEEHEATSNERKG---KPDFLDFIMANRDNSEGER-LSITNIK 301

Query: 242 ALLMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYL 301
           ALL+++   GTDTSS+ IE+A+ EMMKNP + +KA++E+  ++G+     ES I +LPYL
Sbjct: 302 ALLLNLFTAGTDTSSSVIEWALTEMMKNPTIFKKAQQEMDQIIGKNRRFIESDIPNLPYL 361

Query: 302 KAIMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFD 361
           +AI KE  R HP  PL +P   SD   +  Y IPK +R+ VN+WAI RDP+ WENPLEF 
Sbjct: 362 RAICKEAFRKHPSTPLNLPRVSSDACTIDGYYIPKNTRLSVNIWAIGRDPDVWENPLEFI 421

Query: 362 PERFM---NGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKL-EEG 421
           PERF+   N K++  G+DF   PFG+GRR C G  MG  MV Y+L TL+HSFDWKL  + 
Sbjct: 422 PERFLSEKNAKIEHRGNDFELIPFGAGRRICAGTRMGIVMVEYILGTLIHSFDWKLPNDV 481

Query: 422 EEIEIEEKFGIVLTMKKPLVLIPTPRLS 443
            +I +EE FG+ L    PL  I TPRLS
Sbjct: 482 VDINMEETFGLALQKAVPLEAIVTPRLS 503

[Close]

BLAST of Cucsa.302200 vs. Swiss-Prot
Match: C75A6_CAMME (Flavonoid 3',5'-hydroxylase OS=Campanula medium GN=CYP75A6 PE=2 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 3.2e-88
Identity = 174/456 (38.16%), Postives = 267/456 (58.55%), Query Frame = 1


Query: 2   ELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDITW 61
           ++  KYG I+ L+LG K  ++ ++P  AR  LK HD  F+NR +        Y   D+ +
Sbjct: 64  DMAVKYGPIMYLKLGSKGTVVASNPKAARAFLKTHDANFSNRPIDGGPTYLAYNAQDMVF 123

Query: 62  TPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIG----EQV 121
             YGP+W++LRK+C++ +LG  +L+    ++ SEV   + ++Y+++  +V +     E +
Sbjct: 124 AEYGPKWKLLRKLCSLHMLGPKALEDWAHVKVSEVGHMLKEMYEQSSKSVPVPVVVPEML 183

Query: 122 FFTVFNVITSMLWG-------GTMEGEEKAAVAVV-FREMVSAMTELAGKPNISDFFPSL 181
            + + N+I  ++           ++    A+ +V  F+ MV  +  +AG  NI DF P +
Sbjct: 184 TYAMANMIGRIILSRRPFVITSKLDSSASASASVSEFQYMVMELMRMAGLFNIGDFIPYI 243

Query: 182 ACLDVQGIEKKMLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQ 241
           A +D+QGI++ M  +  K D +  K+I E    T +    ++  DFL  L+    E+   
Sbjct: 244 AWMDLQGIQRDMKVIQKKFDVLLNKMIKEH---TESAHDRKDNPDFLDILMAATQENTEG 303

Query: 242 TPLTVVQLKALLMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEE 301
             L +V +KALL+D+   GTDTSS+ IE+A+AEM+ + ++  +A EE+  V+G    +E+
Sbjct: 304 IQLNLVNVKALLLDLFTAGTDTSSSVIEWALAEMLNHRQILNRAHEEMDQVIGRNRRLEQ 363

Query: 302 SHIQSLPYLKAIMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPN 361
           S I +LPY +AI KET R HP  PL +P   ++   V  + IPK +R+ VN+WAI RDP 
Sbjct: 364 SDIPNLPYFQAICKETFRKHPSTPLNLPRISTEACEVDGFHIPKNTRLIVNIWAIGRDPK 423

Query: 362 EWENPLEFDPERFM---NGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHS 421
            WENPL+F PERF+   + K+D  G+ F   PFG+GRR C G  MG   V Y+L TL+HS
Sbjct: 424 VWENPLDFTPERFLSEKHAKIDPRGNHFELIPFGAGRRICAGARMGAASVEYILGTLVHS 483

Query: 422 FDWKLEEG-EEIEIEEKFGIVLTMKKPLVLIPTPRL 442
           FDWKL +G  E+ +EE FGI L  K PL  I TPRL
Sbjct: 484 FDWKLPDGVVEVNMEESFGIALQKKVPLSAIVTPRL 516

[Close]

BLAST of Cucsa.302200 vs. Swiss-Prot
Match: C75A1_PETHY (Flavonoid 3',5'-hydroxylase 1 OS=Petunia hybrida GN=CYP75A1 PE=2 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 9.3e-88
Identity = 183/447 (40.94%), Postives = 268/447 (59.96%), Query Frame = 1


Query: 2   ELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRV-ATYGGFDIT 61
           ++ +KYG+I+ L++G     + ++P  A+  LK  DI F+NR  P AG     Y   D+ 
Sbjct: 59  KMAKKYGAIMYLKVGTCGMAVASTPDAAKAFLKTLDINFSNRP-PNAGATHLAYNAQDMV 118

Query: 62  WTPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQL--YQRAESTVKIGEQVF 121
           +  YGP W++LRK+  + +LG  +L+    +R +E+   +  +    R    V + E + 
Sbjct: 119 FAHYGPRWKLLRKLSNLHMLGGKALENWANVRANELGHMLKSMSDMSREGQRVVVAEMLT 178

Query: 122 FTVFNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIE 181
           F + N+I  ++    +   +K      F++MV  +  +AG  NI DF P LA +D+QGIE
Sbjct: 179 FAMANMIGQVMLSKRVF-VDKGVEVNEFKDMVVELMTIAGYFNIGDFIPCLAWMDLQGIE 238

Query: 182 KKMLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQLK 241
           K+M +L  K D +  K+ DE    T   +G   K DFL  +++  D S+ +  L+   +K
Sbjct: 239 KRMKRLHKKFDALLTKMFDEHKATTYERKG---KPDFLDVVMENGDNSEGER-LSTTNIK 298

Query: 242 ALLMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYL 301
           ALL+++   GTDTSS+ IE+A+AEMMKNP + +KA+ E+  V+G    + ES I +LPYL
Sbjct: 299 ALLLNLFTAGTDTSSSAIEWALAEMMKNPAILKKAQAEMDQVIGRNRRLLESDIPNLPYL 358

Query: 302 KAIMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFD 361
           +AI KET R HP  PL +P   ++  +V  Y IPK +R+ VN+WAI RDP  WENPLEF+
Sbjct: 359 RAICKETFRKHPSTPLNLPRISNEPCIVDGYYIPKNTRLSVNIWAIGRDPQVWENPLEFN 418

Query: 362 PERFMNG---KLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKL-EEG 421
           PERF++G   K+D  G+DF   PFG+GRR C G  MG  MV Y+L TL+HSFDWKL  E 
Sbjct: 419 PERFLSGRNSKIDPRGNDFELIPFGAGRRICAGTRMGIVMVEYILGTLVHSFDWKLPSEV 478

Query: 422 EEIEIEEKFGIVLTMKKPLVLIPTPRL 442
            E+ +EE FG+ L    PL  + TPRL
Sbjct: 479 IELNMEEAFGLALQKAVPLEAMVTPRL 499

[Close]

BLAST of Cucsa.302200 vs. Swiss-Prot
Match: C75A3_PETHY (Flavonoid 3',5'-hydroxylase 2 OS=Petunia hybrida GN=CYP75A3 PE=2 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 2.1e-87
Identity = 182/447 (40.72%), Postives = 268/447 (59.96%), Query Frame = 1


Query: 2   ELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRV-ATYGGFDIT 61
           ++ +KYG+I+ L++G    ++ ++P  A+  LK  D+ F+NR  P AG     YG  D+ 
Sbjct: 59  KMAKKYGAIMYLKVGTCGMVVASTPDAAKAFLKTLDLNFSNRP-PNAGATHLAYGAQDMV 118

Query: 62  WTPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQ--RAESTVKIGEQVF 121
           +  YGP W++LRK+  + +LG  +L+    +R +E+   +  ++   R    V + E + 
Sbjct: 119 FAHYGPRWKLLRKLSNLHMLGGKALENWANVRANELGHMLKSMFDMSREGERVVVAEMLT 178

Query: 122 FTVFNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIE 181
           F + N+I  ++    +    K      F++MV  +   AG  NI DF P LA +D+QGIE
Sbjct: 179 FAMANMIGQVILSKRVF-VNKGVEVNEFKDMVVELMTTAGYFNIGDFIPCLAWMDLQGIE 238

Query: 182 KKMLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQLK 241
           K M +L  K D +  K+ DE    +   +G   K DFL  +++ +D S+ +  L+   +K
Sbjct: 239 KGMKRLHKKFDALLTKMFDEHKATSYERKG---KPDFLDCVMENRDNSEGER-LSTTNIK 298

Query: 242 ALLMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYL 301
           ALL+++   GTDTSS+ IE+A+AEMMKNP + +KA+ E+  V+G    + ES I +LPYL
Sbjct: 299 ALLLNLFTAGTDTSSSAIEWALAEMMKNPAILKKAQGEMDQVIGNNRRLLESDIPNLPYL 358

Query: 302 KAIMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFD 361
           +AI KET R HP  PL +P   ++  +V  Y IPK +R+ VN+WAI RDP  WENPLEF 
Sbjct: 359 RAICKETFRKHPSTPLNLPRISNEPCIVDGYYIPKNTRLSVNIWAIGRDPEVWENPLEFY 418

Query: 362 PERFMNG---KLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKL-EEG 421
           PERF++G   K+D  G+DF   PFG+GRR C G  MG  MV Y+L TL+HSFDWKL  E 
Sbjct: 419 PERFLSGRNSKIDPRGNDFELIPFGAGRRICAGTRMGIVMVEYILGTLVHSFDWKLPSEV 478

Query: 422 EEIEIEEKFGIVLTMKKPLVLIPTPRL 442
            E+ +EE FG+ L    PL  + TPRL
Sbjct: 479 IELNMEEAFGLALQKAVPLEAMVTPRL 499

[Close]

BLAST of Cucsa.302200 vs. Swiss-Prot
Match: F3PH_PETHY (Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 3.5e-87
Identity = 176/451 (39.02%), Postives = 262/451 (58.09%), Query Frame = 1


Query: 3   LGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDITWT 62
           + Q YG ++ L++G    ++  S SVA + LK HD  F++R          Y   D+ + 
Sbjct: 60  MAQTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFA 119

Query: 63  PYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQVFFTVF 122
           PYGP WRMLRK+C++ L    +LD    +R+ EV+     L    +  VK+G+ +     
Sbjct: 120 PYGPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNVCTT 179

Query: 123 NVITSMLWGGTMEGEEKAAV---AVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEK 182
           N +  ++ G  +  +    V   A  F+ MV  M  +AG  NI DF P L  LD+QG+  
Sbjct: 180 NALARVMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAA 239

Query: 183 KMLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVK-DESDSQT-PLTVVQL 242
           KM KL  + D     +++E       E       D L  L+ +K D++D+    LT  ++
Sbjct: 240 KMKKLHARFDAFLTDILEEHKGKIFGE-----MKDLLSTLISLKNDDADNDGGKLTDTEI 299

Query: 243 KALLMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPY 302
           KALL+++   GTDTSS+T+E+A+AE+++NPK+  +A++E+  VVG   +V E  +  L Y
Sbjct: 300 KALLLNLFVAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQLTY 359

Query: 303 LKAIMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEF 362
           L+AI+KET RLHP  PL +P   S++  ++ Y IPKGS + +NVWAI RDPN W +PLEF
Sbjct: 360 LEAIVKETFRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADPLEF 419

Query: 363 DPERFMNG----KLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEE 422
            PERF+ G    K+D  G+DF   PFG+GRR C G+ +G RMV  ++ATL+H+F+W L  
Sbjct: 420 RPERFLPGGEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDLVS 479

Query: 423 G---EEIEIEEKFGIVLTMKKPLVLIPTPRL 442
           G   E + +EE +G+ L    PLV+ P PRL
Sbjct: 480 GQLPEMLNMEEAYGLTLQRADPLVVHPRPRL 505

[Close]

BLAST of Cucsa.302200 vs. TrEMBL
Match: A0A0A0KP59_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G159610 PE=3 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 1.6e-256
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 1


Query: 1   MELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDIT 60
           MELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDIT
Sbjct: 67  MELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDIT 126

Query: 61  WTPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQVFFT 120
           WTPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQVFFT
Sbjct: 127 WTPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQVFFT 186

Query: 121 VFNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEKK 180
           VFNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEKK
Sbjct: 187 VFNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEKK 246

Query: 181 MLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQLKAL 240
           MLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQLKAL
Sbjct: 247 MLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQLKAL 306

Query: 241 LMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYLKA 300
           LMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYLKA
Sbjct: 307 LMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYLKA 366

Query: 301 IMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFDPE 360
           IMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFDPE
Sbjct: 367 IMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFDPE 426

Query: 361 RFMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEEIEIE 420
           RFMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEEIEIE
Sbjct: 427 RFMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEEIEIE 486

Query: 421 EKFGIVLTMKKPLVLIPTPRLSDPTLYQ 449
           EKFGIVLTMKKPLVLIPTPRLSDPTLYQ
Sbjct: 487 EKFGIVLTMKKPLVLIPTPRLSDPTLYQ 514

[Close]

BLAST of Cucsa.302200 vs. TrEMBL
Match: A0A0A0KKS3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G159620 PE=3 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 1.3e-202
Identity = 352/448 (78.57%), Postives = 394/448 (87.95%), Query Frame = 1


Query: 2   ELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDITW 61
           ELG+KYG IV+LQLGGK+GIIVNS S+ARE+LKDHDITFAN DV +AGR ATYGG DI+W
Sbjct: 71  ELGRKYGPIVRLQLGGKIGIIVNSSSLAREVLKDHDITFANHDVHEAGRAATYGGSDISW 130

Query: 62  TPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQVFFTV 121
            PYGPEWRMLRKVCTIK+L NASLD VYELRR EVR TVA LY+RA S V +GEQ F T+
Sbjct: 131 APYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTVAHLYRRAGSAVNVGEQGFLTI 190

Query: 122 FNVITSMLWGGTMEGEE-KAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEKK 181
           FNV+TSMLWGG++E ++ +  +A  FRE +S +T+L G+PN+SDFFPSLA  D+QGIEK+
Sbjct: 191 FNVVTSMLWGGSVESDQSRDNIAAEFRETISEITKLLGQPNVSDFFPSLARFDLQGIEKQ 250

Query: 182 MLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQLKAL 241
           M+K + KLDTIFEK+IDER+RM +  E S  KNDFLQFLL VKDE DS+TPLT+VQLK L
Sbjct: 251 MVKHVLKLDTIFEKMIDERVRMKDVNE-SVKKNDFLQFLLTVKDEGDSKTPLTMVQLKGL 310

Query: 242 LMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYLKA 301
           LMDMV GGTDTSSNT+EFAM EMMKNPK+ EKAKEEL AVVGEQ +VEESHIQSL YLKA
Sbjct: 311 LMDMVTGGTDTSSNTVEFAMGEMMKNPKILEKAKEELCAVVGEQRMVEESHIQSLSYLKA 370

Query: 302 IMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFDPE 361
           +MKETLRLHPILPLL+PHCPS+TTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPL FDPE
Sbjct: 371 VMKETLRLHPILPLLIPHCPSETTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLVFDPE 430

Query: 362 RFMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEEIEIE 421
           RF+NGK DFSGSDFRYFPFGSGRR C GIAM ER VMYLLATLLHSFDWKLEEGE+IE+E
Sbjct: 431 RFLNGKFDFSGSDFRYFPFGSGRRNCAGIAMAERTVMYLLATLLHSFDWKLEEGEKIEVE 490

Query: 422 EKFGIVLTMKKPLVLIPTPRLSDPTLYQ 449
           EKFGIVL MKKPLVLIP PRL DPTLY+
Sbjct: 491 EKFGIVLKMKKPLVLIPRPRLLDPTLYE 517

[Close]

BLAST of Cucsa.302200 vs. TrEMBL
Match: A0A0A0KR55_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G159600 PE=3 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 2.5e-201
Identity = 341/449 (75.95%), Postives = 393/449 (87.53%), Query Frame = 1


Query: 2   ELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDITW 61
           +LGQKYG IVKLQLG K+GII+NSPSV RE+LKDHD+TFANRDVPQAGR A+YGG DI W
Sbjct: 65  QLGQKYGPIVKLQLGRKIGIIINSPSVVREVLKDHDVTFANRDVPQAGRAASYGGSDIVW 124

Query: 62  TPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQVFFTV 121
           TPYGP+WRMLRKVC +K+L NA+LD VYELRR EVR TVA LY RA + V +GEQ F TV
Sbjct: 125 TPYGPKWRMLRKVCVVKMLSNATLDSVYELRRKEVRNTVAHLYARAGTAVNVGEQGFLTV 184

Query: 122 FNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEKKM 181
           FNV+TSMLWGG++EGE++  +A  FRE VS MTEL G PN+SDFFPSLA  D+QGIEKKM
Sbjct: 185 FNVVTSMLWGGSVEGEQRDGLAAEFRETVSEMTELLGLPNVSDFFPSLARFDLQGIEKKM 244

Query: 182 LKLLPKLDTIFEKLIDERLRMTNNEEG-SRNKNDFLQFLLKVKDESDSQTPLTVVQLKAL 241
            +L P+ D+IFEK+ID+RL++   +EG S  KNDFLQFLL+VKDE +S+TPLT+  LKAL
Sbjct: 245 RELAPRFDSIFEKMIDQRLKIDGGDEGGSVKKNDFLQFLLQVKDEEESKTPLTMTHLKAL 304

Query: 242 LMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYLKA 301
           LMDMV GGTDTSSNT+EFAMAEM+K+PK  +KA+EE+ AVVGE +IVEESHI SLPYLKA
Sbjct: 305 LMDMVIGGTDTSSNTVEFAMAEMLKSPKTLKKAQEEVVAVVGEDNIVEESHIHSLPYLKA 364

Query: 302 IMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFDPE 361
           +MKETLRLHPILPLLVPHCPS+T +VSNYTIPKGSRVF+NVWAIQRDP  W+NPLEFDPE
Sbjct: 365 VMKETLRLHPILPLLVPHCPSETAIVSNYTIPKGSRVFINVWAIQRDPKNWDNPLEFDPE 424

Query: 362 RFMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGE-EIEI 421
           RF+NGK DFSG+DFRYFPFGSGRR C GIAM ERMVMY+LATLLHSFDWKLEEG+ +IE+
Sbjct: 425 RFLNGKFDFSGNDFRYFPFGSGRRNCAGIAMAERMVMYMLATLLHSFDWKLEEGDGKIEV 484

Query: 422 EEKFGIVLTMKKPLVLIPTPRLSDPTLYQ 449
           EEKFGIVL MK PLVLIPTP+L+DPTLYQ
Sbjct: 485 EEKFGIVLKMKTPLVLIPTPKLADPTLYQ 513

[Close]

BLAST of Cucsa.302200 vs. TrEMBL
Match: M5WU51_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005322mg PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 9.1e-175
Identity = 295/447 (66.00%), Postives = 360/447 (80.54%), Query Frame = 1


Query: 3   LGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDITWT 62
           L Q +G I KL+LG  +G++V S S AREILKDHD+TFANRDVP AGR A YGG DI WT
Sbjct: 23  LAQAHGPIFKLRLGNTLGVVVTSASSAREILKDHDVTFANRDVPAAGRAAAYGGNDIVWT 82

Query: 63  PYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQVFFTVF 122
           PYGPEWRMLRKVC +K+L N +LD VYELRR ++R+TV   Y R  S V +GEQ+F  V 
Sbjct: 83  PYGPEWRMLRKVCVLKMLSNTTLDSVYELRRKQLRETVGYFYSRVGSPVNVGEQMFLNVL 142

Query: 123 NVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEKKML 182
           NVIT+MLWGGT+EG+E+A +   FRE+VS MTEL GKPN+SDF+P LA  D+QG+EK+M 
Sbjct: 143 NVITNMLWGGTVEGDERAGLGAEFREVVSEMTELLGKPNVSDFYPGLARFDLQGVEKQMS 202

Query: 183 KLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDES-DSQTPLTVVQLKALL 242
           +L  + D IFEK+ID+R+R+  ++EG     DFL FLLK+KDE  DS+TP T+  LKALL
Sbjct: 203 RLARRFDGIFEKIIDQRMRI--DKEGPTESKDFLTFLLKLKDEGGDSKTPFTITHLKALL 262

Query: 243 MDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYLKAI 302
           MDMV GG+DTSSNT+EFA++E+M  P+V +KA++EL AVVG+ +IVEESHI  LPYL+A+
Sbjct: 263 MDMVVGGSDTSSNTVEFALSEIMNKPEVMKKAQQELEAVVGKHNIVEESHIHKLPYLQAV 322

Query: 303 MKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFDPER 362
           MKETLRLHP LPLLVPHCPS+T  V  YTIPKGSR+F NVWAI RDP+ WENPLEFDPER
Sbjct: 323 MKETLRLHPALPLLVPHCPSETCTVGGYTIPKGSRIFFNVWAIHRDPSIWENPLEFDPER 382

Query: 363 FMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEEIEIEE 422
           F+N K D+SG+DF YFPFGSGRR C GIAM ERMVM+ L+TL+HSFDWK+ +G+++++ E
Sbjct: 383 FLNSKWDYSGNDFNYFPFGSGRRICAGIAMAERMVMHSLSTLVHSFDWKVPQGQKLDLSE 442

Query: 423 KFGIVLTMKKPLVLIPTPRLSDPTLYQ 449
           KFGIVL  K PLV IPTPRLSDP LYQ
Sbjct: 443 KFGIVLKKKMPLVAIPTPRLSDPALYQ 467

[Close]

BLAST of Cucsa.302200 vs. TrEMBL
Match: U5GNW2_POPTR (Cytochrome P450 family protein OS=Populus trichocarpa GN=POPTR_0001s07850g PE=3 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 7.2e-172
Identity = 285/446 (63.90%), Postives = 356/446 (79.82%), Query Frame = 1


Query: 3   LGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDITWT 62
           L + YG I+KLQLG K+GIIV+SP++ARE+LKDHDITFANRDVP   R+A YGG DI W+
Sbjct: 61  LARTYGPILKLQLGSKLGIIVSSPNLAREVLKDHDITFANRDVPDVARIAAYGGSDIAWS 120

Query: 63  PYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQVFFTVF 122
           PYGPEWRMLRKVC +K+L N++LD VYELRR EVR  +A +Y +  S + +GEQ F T+ 
Sbjct: 121 PYGPEWRMLRKVCVLKMLSNSTLDSVYELRRREVRNIIAYIYSKPGSPINVGEQTFLTIL 180

Query: 123 NVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEKKML 182
           NV+TSMLWGGT++GEE+ ++   FR +V+ MTEL G PNISDFFP+LA  D+QG+ KKM 
Sbjct: 181 NVVTSMLWGGTVQGEERGSLGAEFRRVVADMTELLGAPNISDFFPALARFDLQGLVKKMS 240

Query: 183 KLLPKLDTIFEKLIDERLRMTN-NEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQLKALL 242
            L PK D IF+++I+++L +    +    +  DFLQFLLKVKDE D +TPLT+  +KALL
Sbjct: 241 GLAPKFDQIFDRMIEKQLSIDALGDRAGASSKDFLQFLLKVKDEGDVKTPLTMTHIKALL 300

Query: 243 MDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYLKAI 302
           MDMV GG+DTSSN IEFA AE+M  P+V  KA++EL  VVG+ +IVEESHI  LPYL AI
Sbjct: 301 MDMVVGGSDTSSNAIEFAFAEVMNKPEVMRKAQDELDRVVGKDNIVEESHIHKLPYLHAI 360

Query: 303 MKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFDPER 362
           MKE+LRLHP+LPLL+PHCPS+T  +  +++PKG+RVF+NVWA+ RDP+ WENPLEF PER
Sbjct: 361 MKESLRLHPVLPLLIPHCPSETCTIGGFSVPKGARVFINVWAVHRDPSIWENPLEFKPER 420

Query: 363 FMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEEIEIEE 422
           F+N K D+SGSDF YFPFGSGRR C GIAM ERM +Y LATLLHSFDWKL EG+++++ E
Sbjct: 421 FLNSKFDYSGSDFNYFPFGSGRRICAGIAMAERMFLYFLATLLHSFDWKLPEGKQMDLTE 480

Query: 423 KFGIVLTMKKPLVLIPTPRLSDPTLY 448
           KFGIVL +K PLV IPTPRLS+P LY
Sbjct: 481 KFGIVLKLKNPLVAIPTPRLSNPALY 506

[Close]

BLAST of Cucsa.302200 vs. TAIR10
Match: AT4G12300.1 (AT4G12300.1 cytochrome P450, family 706, subfamily A, polypeptide 4)

HSP 1 Score: 534.6 bits (1376), Expect = 5.7e-152
Identity = 259/449 (57.68%), Postives = 336/449 (74.83%), Query Frame = 1


Query: 3   LGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDITWT 62
           L Q +G I KL LG K+ I+VNSPS+AREILKD DI F+NRDVP  GR ATYGG DI WT
Sbjct: 69  LAQSHGPIFKLNLGSKLTIVVNSPSLAREILKDQDINFSNRDVPLTGRAATYGGIDIVWT 128

Query: 63  PYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQ--RAESTVKIGEQVFFT 122
           PYG EWR LRK+C +KLL   +LD  YELRR EVR+    LY+  R +S VK+G+Q+F T
Sbjct: 129 PYGAEWRQLRKICVLKLLSRKTLDSFYELRRKEVRERTRYLYEQGRKQSPVKVGDQLFLT 188

Query: 123 VFNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEKK 182
           + N+  +MLWGG+++ EE  +V   F+ ++S +T L  +PN+SDFFP LA  D+QG+ K+
Sbjct: 189 MMNLTMNMLWGGSVKAEEMESVGTEFKGVISEITRLLSEPNVSDFFPWLARFDLQGLVKR 248

Query: 183 MLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKD-ESDSQTPLTVVQLKA 242
           M     +LD + ++ I++   +   ++      DFLQ+L+K+KD E DS+ P+T+  +KA
Sbjct: 249 MGVCARELDAVLDRAIEQMKPLRGRDDDE--VKDFLQYLMKLKDQEGDSEVPITINHVKA 308

Query: 243 LLMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYLK 302
           LL DMV GGTDTS+NTIEFAMAE+M NP++ ++A+EEL  VVG+ +IVEESHI  LPY+ 
Sbjct: 309 LLTDMVVGGTDTSTNTIEFAMAELMSNPELIKRAQEELDEVVGKDNIVEESHITRLPYIL 368

Query: 303 AIMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFDP 362
           AIMKETLRLHP LPLLVPH P++ TVV  YTIPK +++FVNVW+IQRDPN WENP EF P
Sbjct: 369 AIMKETLRLHPTLPLLVPHRPAENTVVGGYTIPKDTKIFVNVWSIQRDPNVWENPTEFRP 428

Query: 363 ERFM-NGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEEIE 422
           ERF+ N   DF+G+++ YFPFGSGRR C G+A+ ERMV+Y LATLLHSFDWK+ EG  ++
Sbjct: 429 ERFLDNNSCDFTGANYSYFPFGSGRRICAGVALAERMVLYTLATLLHSFDWKIPEGHVLD 488

Query: 423 IEEKFGIVLTMKKPLVLIPTPRLSDPTLY 448
           ++EKFGIVL +K PLV +P PR SD  LY
Sbjct: 489 LKEKFGIVLKLKIPLVALPIPRFSDSNLY 515

[Close]

BLAST of Cucsa.302200 vs. TAIR10
Match: AT4G12320.1 (AT4G12320.1 cytochrome P450, family 706, subfamily A, polypeptide 6)

HSP 1 Score: 523.5 bits (1347), Expect = 1.3e-148
Identity = 248/450 (55.11%), Postives = 336/450 (74.67%), Query Frame = 1


Query: 2   ELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDITW 61
           +L + YG I KL LG K+ ++VN+PS+AREILKD DI F+N DVP   R  TYGG D+ W
Sbjct: 68  KLAESYGPIFKLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVW 127

Query: 62  TPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRA--ESTVKIGEQVFF 121
            PYG EWRMLRKVC +KLL + +L+  YELRR E+R+    LYQ+   ES V +GEQVF 
Sbjct: 128 LPYGAEWRMLRKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFL 187

Query: 122 TVFNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEK 181
           T+ N+  +MLWGG+++ EE  +V   F+E++S +T L G+PN+SDFFP LA  D+QG+ K
Sbjct: 188 TMMNLTMNMLWGGSVKAEEMESVGTEFKEVISEITRLLGEPNVSDFFPRLARFDLQGLVK 247

Query: 182 KMLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKD-ESDSQTPLTVVQLK 241
           KM     +LD I ++ I++   +   +       DFLQ L+K+KD E+DS+ P+TV  +K
Sbjct: 248 KMHVCARELDAILDRAIEQMQLLRTRDGDDGECKDFLQHLMKLKDQEADSEVPITVNHVK 307

Query: 242 ALLMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYL 301
           A+L+D+V GGTDTS+NTIEFAMAE+++ P++ ++A++EL  VVG+ +I+EESHI  LP++
Sbjct: 308 AVLVDLVVGGTDTSTNTIEFAMAELIRKPELMKRAQQELDEVVGKDNIIEESHITRLPFI 367

Query: 302 KAIMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFD 361
            AIMKETLRL+P +PLLVPH PS+T +V  YTIPK +++F+NVW+IQRDPN WE P EF 
Sbjct: 368 SAIMKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFR 427

Query: 362 PERFMNGK-LDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEEI 421
           PERF++ K  DF+G+D+ Y PFGSGRR C GIA+ ERM++Y LATLLHSFDWK+ EG  +
Sbjct: 428 PERFLDKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWKIPEGHIL 487

Query: 422 EIEEKFGIVLTMKKPLVLIPTPRLSDPTLY 448
           +++EKFGIVL +K PLV +P PRLS+  LY
Sbjct: 488 DLKEKFGIVLKLKSPLVALPVPRLSNSNLY 517

[Close]

BLAST of Cucsa.302200 vs. TAIR10
Match: AT4G12310.1 (AT4G12310.1 cytochrome P450, family 706, subfamily A, polypeptide 5)

HSP 1 Score: 508.8 bits (1309), Expect = 3.3e-144
Identity = 242/450 (53.78%), Postives = 328/450 (72.89%), Query Frame = 1


Query: 2   ELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDITW 61
           +L Q +G I KL LG K+ ++VNSPS+A EILKD DI F+N DVP   R  TYGG D+ W
Sbjct: 70  KLAQSHGPIFKLNLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLVW 129

Query: 62  TPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRA--ESTVKIGEQVFF 121
            PYG EWRMLRKVC  KL    +LD  YELRR E+R+    LYQ+   +S V +GEQ+F 
Sbjct: 130 LPYGAEWRMLRKVCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFL 189

Query: 122 TVFNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEK 181
           T+ N++ +MLWGG+++ E+  +V   F+ ++S +T L G PN+SDFFP LA  D+QG+ K
Sbjct: 190 TMMNLMMNMLWGGSVKAEDMESVGTEFKGVISEITRLLGVPNVSDFFPMLARFDLQGLVK 249

Query: 182 KMLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKD-ESDSQTPLTVVQLK 241
           KM      LD I ++ I++  R+ + +       DFLQ L+K++D E+DS  P+T+  +K
Sbjct: 250 KMHLYARDLDAILDRAIEQMQRLRSRDGDDGECKDFLQHLMKLRDQEADSDVPITMNHVK 309

Query: 242 ALLMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYL 301
           A+LMDMV GGT++S+NTIEF MAE++ NP++  +A++EL  VVG+ +IVEESHI SLPY+
Sbjct: 310 AVLMDMVVGGTESSTNTIEFVMAELISNPELMRRAQQELDEVVGKDNIVEESHITSLPYI 369

Query: 302 KAIMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFD 361
            A++KETLRL+P +PLLVPH PS+T +V  YTIPK +++F+NVW+IQRDPN WE P EF 
Sbjct: 370 LAVLKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFR 429

Query: 362 PERFMNGK-LDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEEI 421
           PERF++ K  DF+G+D+ Y PFGSGRR C GIA+ ERM++Y LATLLHSFDW + +G  +
Sbjct: 430 PERFLDKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWTIPDGHVL 489

Query: 422 EIEEKFGIVLTMKKPLVLIPTPRLSDPTLY 448
           ++EEKFGIVL +K PLV +P PRLS+   Y
Sbjct: 490 DLEEKFGIVLKLKTPLVALPIPRLSNSNFY 519

[Close]

BLAST of Cucsa.302200 vs. TAIR10
Match: AT5G44620.1 (AT5G44620.1 cytochrome P450, family 706, subfamily A, polypeptide 3)

HSP 1 Score: 507.3 bits (1305), Expect = 9.7e-144
Identity = 249/447 (55.70%), Postives = 324/447 (72.48%), Query Frame = 1


Query: 3   LGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDITWT 62
           L +K+G I KL LG K+ I+V S  VA+EILK +DI FAN DVP  G V TYGG +I W+
Sbjct: 73  LAKKHGPIFKLWLGAKLTIVVTSSEVAQEILKTNDIIFANHDVPAVGPVNTYGGTEIIWS 132

Query: 63  PYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAE--STVKIGEQVFFT 122
           PYGP+WRMLRK+C  ++L NA LD   +LRR E R+TV  L  +A   S V +GEQ+F  
Sbjct: 133 PYGPKWRMLRKLCVNRILRNAMLDSSTDLRRRETRQTVRYLADQARVGSPVNLGEQIFLM 192

Query: 123 VFNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEKK 182
           + NV+T MLWG T++ EE+  V   F E++  M +L   PNISDFFP L+  D+QG+ K+
Sbjct: 193 MLNVVTQMLWGTTVKEEEREVVGAEFLEVIREMNDLLLVPNISDFFPVLSRFDLQGLAKR 252

Query: 183 MLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQLKAL 242
           M +   ++D +F+++I++RL M  +    R   DFL  LLKVKDE   +T LT+  +KA+
Sbjct: 253 MRRPAQRMDQMFDRIINQRLGMDRDSSDGRAV-DFLDVLLKVKDEEAEKTKLTMNDVKAV 312

Query: 243 LMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYLKA 302
           LMDMV GGTDTS + IEFAMAE++ NP + ++A++E+  VVG++ +VEESHI  LPY+ A
Sbjct: 313 LMDMVLGGTDTSLHVIEFAMAELLHNPDIMKRAQQEVDKVVGKEKVVEESHISKLPYILA 372

Query: 303 IMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFDPE 362
           IMKETLRLH + PLLVP  PS TTVV  +TIPK S++F+N WAI R+PN WENPL+FDP+
Sbjct: 373 IMKETLRLHTVAPLLVPRRPSQTTVVGGFTIPKDSKIFINAWAIHRNPNVWENPLKFDPD 432

Query: 363 RFMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEEIEIE 422
           RF++   DF G+DF Y PFGSGRR C G+AMGER+V+Y LAT LHSFDWK+ +GE +E+E
Sbjct: 433 RFLDMSYDFKGNDFNYLPFGSGRRICVGMAMGERVVLYNLATFLHSFDWKIPQGERVEVE 492

Query: 423 EKFGIVLTMKKPLVLIPTPRLSDPTLY 448
           EKFGIVL +K PLV  P  RLSDP LY
Sbjct: 493 EKFGIVLELKNPLVATPVLRLSDPNLY 518

[Close]

BLAST of Cucsa.302200 vs. TAIR10
Match: AT4G12330.1 (AT4G12330.1 cytochrome P450, family 706, subfamily A, polypeptide 7)

HSP 1 Score: 502.3 bits (1292), Expect = 3.1e-142
Identity = 242/450 (53.78%), Postives = 330/450 (73.33%), Query Frame = 1


Query: 3   LGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDITWT 62
           L QK+G + KL LG K+ I++ S    R+IL+ +D+ FAN DVP AG ++TYGG DI W+
Sbjct: 73  LAQKHGPVFKLWLGAKLTIVITSSEATRDILRTNDVIFANDDVPVAGSLSTYGGVDIVWS 132

Query: 63  PYGPEWRMLRKVCTIKLLGNASLDM--VYELRRSEVRKTVAQLYQRAES--TVKIGEQVF 122
           PYGPEW MLRK+C  K+L NA+LD      LRR E R+TV  L  RA +   V +GEQ+F
Sbjct: 133 PYGPEWPMLRKICINKMLSNATLDSNSFSALRRQETRRTVRYLADRARAGLAVNVGEQIF 192

Query: 123 FTVFNVITSMLWGGTM-EGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGI 182
            T+ NV+T MLWG T+ + EE+  V   F E+++ + ++ GKPN+SDFFP L+  D+QG+
Sbjct: 193 VTILNVVTQMLWGETVADDEEREKVGAEFLELITEIIDVVGKPNVSDFFPVLSRFDLQGL 252

Query: 183 EKKMLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQL 242
            K++ +   ++D +F+++I +R+ M    +G  N  DFL  LL  KDE ++   +++  +
Sbjct: 253 AKRVRRSAQRMDRMFDRIISQRMGMDKGSKG--NGGDFLMVLLNAKDEDEN---MSMNHV 312

Query: 243 KALLMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPY 302
           KALLMDMV GGTDTS NTIEFAMAE++   ++ ++A++EL  VVG+ +IVEE HI  LPY
Sbjct: 313 KALLMDMVLGGTDTSLNTIEFAMAELINKLEIMKRAQQELDKVVGKNNIVEEKHITKLPY 372

Query: 303 LKAIMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEF 362
           + +IMKETLRLHP LPLL+P CPS+TTV+  YTIP  S+VF+NVWAI R+PN WENPLEF
Sbjct: 373 ILSIMKETLRLHPALPLLIPRCPSETTVIGGYTIPNDSKVFINVWAIHRNPNVWENPLEF 432

Query: 363 DPERFMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEEI 422
           +P+RF++   DFSG+D+ YFPFGSGRR C G+AM E++V+Y LATLLHSFDW++ EGE++
Sbjct: 433 NPDRFLDKGYDFSGNDYSYFPFGSGRRICAGMAMAEKVVLYNLATLLHSFDWRIGEGEKV 492

Query: 423 EIEEKFGIVLTMKKPLVLIPTPRLSDPTLY 448
           E+EEKFGI+L +K PLV  P  RLSDP L+
Sbjct: 493 ELEEKFGILLKLKNPLVATPVLRLSDPNLF 517

[Close]

BLAST of Cucsa.302200 vs. NCBI nr
Match: gi|778699925|ref|XP_011654786.1| (PREDICTED: flavonoid 3',5'-hydroxylase-like [Cucumis sativus])

HSP 1 Score: 892.9 bits (2306), Expect = 2.3e-256
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 1


Query: 1   MELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDIT 60
           MELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDIT
Sbjct: 67  MELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDIT 126

Query: 61  WTPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQVFFT 120
           WTPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQVFFT
Sbjct: 127 WTPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQVFFT 186

Query: 121 VFNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEKK 180
           VFNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEKK
Sbjct: 187 VFNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEKK 246

Query: 181 MLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQLKAL 240
           MLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQLKAL
Sbjct: 247 MLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQLKAL 306

Query: 241 LMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYLKA 300
           LMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYLKA
Sbjct: 307 LMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYLKA 366

Query: 301 IMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFDPE 360
           IMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFDPE
Sbjct: 367 IMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFDPE 426

Query: 361 RFMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEEIEIE 420
           RFMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEEIEIE
Sbjct: 427 RFMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEEIEIE 486

Query: 421 EKFGIVLTMKKPLVLIPTPRLSDPTLYQ 449
           EKFGIVLTMKKPLVLIPTPRLSDPTLYQ
Sbjct: 487 EKFGIVLTMKKPLVLIPTPRLSDPTLYQ 514

[Close]

BLAST of Cucsa.302200 vs. NCBI nr
Match: gi|659073578|ref|XP_008437137.1| (PREDICTED: geraniol 8-hydroxylase-like [Cucumis melo])

HSP 1 Score: 721.5 bits (1861), Expect = 9.3e-205
Identity = 355/448 (79.24%), Postives = 397/448 (88.62%), Query Frame = 1


Query: 2   ELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDITW 61
           ELG+KYG IV+L+LGGK+GIIVNS SVARE+LKDHDITFANRDVPQAGR ATYGG+DI+W
Sbjct: 71  ELGRKYGPIVRLRLGGKIGIIVNSSSVAREVLKDHDITFANRDVPQAGRAATYGGYDISW 130

Query: 62  TPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQVFFTV 121
            PYGPEWRMLRKVCT+K+L NASLD VYELRR EVRKTVA LY+RA S V +GEQ F T+
Sbjct: 131 APYGPEWRMLRKVCTVKMLSNASLDSVYELRRREVRKTVAHLYRRAGSAVNVGEQGFLTI 190

Query: 122 FNVITSMLWGGTMEGEE-KAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEKK 181
           FNV+TSMLWGG++E ++ +  +A  FRE VS +T+L G PN+SDFFPSLA  D+QGIEK+
Sbjct: 191 FNVVTSMLWGGSVENDQSRDDIAADFRETVSDVTKLLGLPNVSDFFPSLARFDLQGIEKQ 250

Query: 182 MLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQLKAL 241
           M K + KLD IFEK+IDERLRM +  E ++ K+DFLQFLLKVKDE DS+TPLT+VQLK L
Sbjct: 251 MGKHVQKLDAIFEKMIDERLRMKDVNESAK-KDDFLQFLLKVKDEGDSKTPLTMVQLKGL 310

Query: 242 LMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYLKA 301
           LMDMV GGTDTSSNTIEFAMAE+MKNPK+ EKAKEEL AVVGEQS+VEESHIQSLPYLKA
Sbjct: 311 LMDMVIGGTDTSSNTIEFAMAEIMKNPKIVEKAKEELCAVVGEQSMVEESHIQSLPYLKA 370

Query: 302 IMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFDPE 361
           +MKE LRLHP LPLLVPHCPS+TTV+SNYTIPKGSR+FVN+WAIQRDPNEWENP  FDPE
Sbjct: 371 VMKEALRLHPTLPLLVPHCPSETTVISNYTIPKGSRMFVNIWAIQRDPNEWENPSVFDPE 430

Query: 362 RFMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEEIEIE 421
           RF+NGK DFSGSDFRY PFGSGRR C GIAM ER VMYLLATLLHSFDWKLEEGE+IEIE
Sbjct: 431 RFLNGKFDFSGSDFRYLPFGSGRRNCAGIAMAERTVMYLLATLLHSFDWKLEEGEKIEIE 490

Query: 422 EKFGIVLTMKKPLVLIPTPRLSDPTLYQ 449
           EKFGIVL MKKPLVLIPTPRL DPTLYQ
Sbjct: 491 EKFGIVLKMKKPLVLIPTPRLLDPTLYQ 517

[Close]

BLAST of Cucsa.302200 vs. NCBI nr
Match: gi|449459696|ref|XP_004147582.1| (PREDICTED: geraniol 8-hydroxylase-like [Cucumis sativus])

HSP 1 Score: 713.8 bits (1841), Expect = 1.9e-202
Identity = 352/448 (78.57%), Postives = 394/448 (87.95%), Query Frame = 1


Query: 2   ELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDITW 61
           ELG+KYG IV+LQLGGK+GIIVNS S+ARE+LKDHDITFAN DV +AGR ATYGG DI+W
Sbjct: 71  ELGRKYGPIVRLQLGGKIGIIVNSSSLAREVLKDHDITFANHDVHEAGRAATYGGSDISW 130

Query: 62  TPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQVFFTV 121
            PYGPEWRMLRKVCTIK+L NASLD VYELRR EVR TVA LY+RA S V +GEQ F T+
Sbjct: 131 APYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTVAHLYRRAGSAVNVGEQGFLTI 190

Query: 122 FNVITSMLWGGTMEGEE-KAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEKK 181
           FNV+TSMLWGG++E ++ +  +A  FRE +S +T+L G+PN+SDFFPSLA  D+QGIEK+
Sbjct: 191 FNVVTSMLWGGSVESDQSRDNIAAEFRETISEITKLLGQPNVSDFFPSLARFDLQGIEKQ 250

Query: 182 MLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQLKAL 241
           M+K + KLDTIFEK+IDER+RM +  E S  KNDFLQFLL VKDE DS+TPLT+VQLK L
Sbjct: 251 MVKHVLKLDTIFEKMIDERVRMKDVNE-SVKKNDFLQFLLTVKDEGDSKTPLTMVQLKGL 310

Query: 242 LMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYLKA 301
           LMDMV GGTDTSSNT+EFAM EMMKNPK+ EKAKEEL AVVGEQ +VEESHIQSL YLKA
Sbjct: 311 LMDMVTGGTDTSSNTVEFAMGEMMKNPKILEKAKEELCAVVGEQRMVEESHIQSLSYLKA 370

Query: 302 IMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFDPE 361
           +MKETLRLHPILPLL+PHCPS+TTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPL FDPE
Sbjct: 371 VMKETLRLHPILPLLIPHCPSETTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLVFDPE 430

Query: 362 RFMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEEIEIE 421
           RF+NGK DFSGSDFRYFPFGSGRR C GIAM ER VMYLLATLLHSFDWKLEEGE+IE+E
Sbjct: 431 RFLNGKFDFSGSDFRYFPFGSGRRNCAGIAMAERTVMYLLATLLHSFDWKLEEGEKIEVE 490

Query: 422 EKFGIVLTMKKPLVLIPTPRLSDPTLYQ 449
           EKFGIVL MKKPLVLIP PRL DPTLY+
Sbjct: 491 EKFGIVLKMKKPLVLIPRPRLLDPTLYE 517

[Close]

BLAST of Cucsa.302200 vs. NCBI nr
Match: gi|449459692|ref|XP_004147580.1| (PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis sativus])

HSP 1 Score: 709.5 bits (1830), Expect = 3.7e-201
Identity = 341/449 (75.95%), Postives = 393/449 (87.53%), Query Frame = 1


Query: 2   ELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDITW 61
           +LGQKYG IVKLQLG K+GII+NSPSV RE+LKDHD+TFANRDVPQAGR A+YGG DI W
Sbjct: 65  QLGQKYGPIVKLQLGRKIGIIINSPSVVREVLKDHDVTFANRDVPQAGRAASYGGSDIVW 124

Query: 62  TPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQVFFTV 121
           TPYGP+WRMLRKVC +K+L NA+LD VYELRR EVR TVA LY RA + V +GEQ F TV
Sbjct: 125 TPYGPKWRMLRKVCVVKMLSNATLDSVYELRRKEVRNTVAHLYARAGTAVNVGEQGFLTV 184

Query: 122 FNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEKKM 181
           FNV+TSMLWGG++EGE++  +A  FRE VS MTEL G PN+SDFFPSLA  D+QGIEKKM
Sbjct: 185 FNVVTSMLWGGSVEGEQRDGLAAEFRETVSEMTELLGLPNVSDFFPSLARFDLQGIEKKM 244

Query: 182 LKLLPKLDTIFEKLIDERLRMTNNEEG-SRNKNDFLQFLLKVKDESDSQTPLTVVQLKAL 241
            +L P+ D+IFEK+ID+RL++   +EG S  KNDFLQFLL+VKDE +S+TPLT+  LKAL
Sbjct: 245 RELAPRFDSIFEKMIDQRLKIDGGDEGGSVKKNDFLQFLLQVKDEEESKTPLTMTHLKAL 304

Query: 242 LMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYLKA 301
           LMDMV GGTDTSSNT+EFAMAEM+K+PK  +KA+EE+ AVVGE +IVEESHI SLPYLKA
Sbjct: 305 LMDMVIGGTDTSSNTVEFAMAEMLKSPKTLKKAQEEVVAVVGEDNIVEESHIHSLPYLKA 364

Query: 302 IMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFDPE 361
           +MKETLRLHPILPLLVPHCPS+T +VSNYTIPKGSRVF+NVWAIQRDP  W+NPLEFDPE
Sbjct: 365 VMKETLRLHPILPLLVPHCPSETAIVSNYTIPKGSRVFINVWAIQRDPKNWDNPLEFDPE 424

Query: 362 RFMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGE-EIEI 421
           RF+NGK DFSG+DFRYFPFGSGRR C GIAM ERMVMY+LATLLHSFDWKLEEG+ +IE+
Sbjct: 425 RFLNGKFDFSGNDFRYFPFGSGRRNCAGIAMAERMVMYMLATLLHSFDWKLEEGDGKIEV 484

Query: 422 EEKFGIVLTMKKPLVLIPTPRLSDPTLYQ 449
           EEKFGIVL MK PLVLIPTP+L+DPTLYQ
Sbjct: 485 EEKFGIVLKMKTPLVLIPTPKLADPTLYQ 513

[Close]

BLAST of Cucsa.302200 vs. NCBI nr
Match: gi|659073580|ref|XP_008437138.1| (PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis melo])

HSP 1 Score: 700.7 bits (1807), Expect = 1.7e-198
Identity = 337/449 (75.06%), Postives = 389/449 (86.64%), Query Frame = 1


Query: 2   ELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGFDITW 61
           +L QKYG IVKLQLG K+GII+NSPSV RE+LKDHD+TFANRDVP AGR A+YGG DI W
Sbjct: 65  QLSQKYGPIVKLQLGRKIGIIINSPSVVREVLKDHDVTFANRDVPHAGRAASYGGSDIVW 124

Query: 62  TPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQVFFTV 121
           TPYGP+WRMLRKVC +K+L NA+LD VYELRR EVR TVA LY RA + V +GEQ F TV
Sbjct: 125 TPYGPQWRMLRKVCVLKMLSNATLDSVYELRRREVRNTVAHLYARAGTRVNVGEQGFLTV 184

Query: 122 FNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQGIEKKM 181
           FNV+TSMLWGG++EGE++  +A  FRE VS MTEL G PN+SDFFPSLA  D+QGIEKKM
Sbjct: 185 FNVVTSMLWGGSVEGEQRDGLAAEFRETVSEMTELLGLPNVSDFFPSLARFDIQGIEKKM 244

Query: 182 LKLLPKLDTIFEKLIDERLRMTNNEEG-SRNKNDFLQFLLKVKDESDSQTPLTVVQLKAL 241
            +L P+ D+IFEK+ID+RL++   +EG S  KNDFLQFLL+VKD+ +S+TPLT+  LKAL
Sbjct: 245 RELAPRFDSIFEKMIDQRLKIDGKDEGESVKKNDFLQFLLQVKDDGESKTPLTMTHLKAL 304

Query: 242 LMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLPYLKA 301
           LMDMV GGTDTSSNT+EFAMAEM+K+PK  +KA++EL AVVGE +IVEESHI SLPYLKA
Sbjct: 305 LMDMVIGGTDTSSNTVEFAMAEMLKSPKTLKKAQQELVAVVGEDNIVEESHIHSLPYLKA 364

Query: 302 IMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLEFDPE 361
           +MKETLRLHPILPLLVPHCPS+T +VSNYTIPKGSRVF+NVWAIQRDP  W+NPLEFDPE
Sbjct: 365 VMKETLRLHPILPLLVPHCPSETAIVSNYTIPKGSRVFINVWAIQRDPKNWDNPLEFDPE 424

Query: 362 RFMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEE-IEI 421
           RF+NGK DFSG+DFRYFPFGSGRR C GI M ERMVMYLLATLLHSFDWKL +G+E IE+
Sbjct: 425 RFLNGKYDFSGNDFRYFPFGSGRRNCAGIGMAERMVMYLLATLLHSFDWKLGDGDEKIEV 484

Query: 422 EEKFGIVLTMKKPLVLIPTPRLSDPTLYQ 449
           EEKFGIVL MK PLV+IPTP+LSDPTLYQ
Sbjct: 485 EEKFGIVLKMKTPLVVIPTPKLSDPTLYQ 513

The following BLAST results are available for this feature:

BLAST of Cucsa.302200 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C75A2_SOLME	2.9e-89	40.18	Flavonoid 3',5'-hydroxylase OS=Solanum melongena GN=CYP75A2 PE=2 SV=1	[more]
C75A6_CAMME	3.2e-88	38.16	Flavonoid 3',5'-hydroxylase OS=Campanula medium GN=CYP75A6 PE=2 SV=1	[more]
C75A1_PETHY	9.3e-88	40.94	Flavonoid 3',5'-hydroxylase 1 OS=Petunia hybrida GN=CYP75A1 PE=2 SV=1	[more]
C75A3_PETHY	2.1e-87	40.72	Flavonoid 3',5'-hydroxylase 2 OS=Petunia hybrida GN=CYP75A3 PE=2 SV=1	[more]
F3PH_PETHY	3.5e-87	39.02	Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=CYP75B2 PE=2 SV=1	[more]

BLAST of Cucsa.302200 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KP59_CUCSA	1.6e-256	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G159610 PE=3 SV=1	[more]
A0A0A0KKS3_CUCSA	1.3e-202	78.57	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G159620 PE=3 SV=1	[more]
A0A0A0KR55_CUCSA	2.5e-201	75.95	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G159600 PE=3 SV=1	[more]
M5WU51_PRUPE	9.1e-175	66.00	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005322mg PE=3 SV=1	[more]
U5GNW2_POPTR	7.2e-172	63.90	Cytochrome P450 family protein OS=Populus trichocarpa GN=POPTR_0001s07850g PE=3 ...	[more]

BLAST of Cucsa.302200 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G12300.1	5.7e-152	57.68	cytochrome P450, family 706, subfamily A, polypeptide 4	[more]
AT4G12320.1	1.3e-148	55.11	cytochrome P450, family 706, subfamily A, polypeptide 6	[more]
AT4G12310.1	3.3e-144	53.78	cytochrome P450, family 706, subfamily A, polypeptide 5	[more]
AT5G44620.1	9.7e-144	55.70	cytochrome P450, family 706, subfamily A, polypeptide 3	[more]
AT4G12330.1	3.1e-142	53.78	cytochrome P450, family 706, subfamily A, polypeptide 7	[more]

BLAST of Cucsa.302200 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778699925\|ref\|XP_011654786.1\|	2.3e-256	100.00	PREDICTED: flavonoid 3',5'-hydroxylase-like [Cucumis sativus]	[more]
gi\|659073578\|ref\|XP_008437137.1\|	9.3e-205	79.24	PREDICTED: geraniol 8-hydroxylase-like [Cucumis melo]	[more]
gi\|449459696\|ref\|XP_004147582.1\|	1.9e-202	78.57	PREDICTED: geraniol 8-hydroxylase-like [Cucumis sativus]	[more]
gi\|449459692\|ref\|XP_004147580.1\|	3.7e-201	75.95	PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis sativus]	[more]
gi\|659073580\|ref\|XP_008437138.1\|	1.7e-198	75.06	PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450
IPR002401	Cyt_P450_E_grp-I
IPR017972	Cyt_P450_CS

Vocabulary: **Molecular Function**
Term	Definition
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506	iron ion binding
GO:0020037	heme binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0033772	flavonoid 3',5'-hydroxylase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.302200.1	Cucsa.302200.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 300..311 score: 1.1E-9coord: 247..264 score: 1.1E-9coord: 377..386 score: 1.
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 2..440 score: 3.5E
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..432 score: 8.0
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 2..440 score: 4.98E
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 4..23 score: 3.5E-48coord: 120..138 score: 3.5E-48coord: 256..282 score: 3.5E-48coord: 386..409 score: 3.5E-48coord: 236..253 score: 3.5E-48coord: 376..386 score: 3.5E-48coord: 28..49 score: 3.5E-48coord: 340..364 score: 3.5E-48coord: 299..317 score: 3.5
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 379..388 scor
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 2..444 score: 9.9E
None	No IPR available	PANTHER	PTHR24298:SF114	CYTOCHROME P450 - LIKE PROTEIN-RELATED	coord: 2..444 score: 9.9E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.302200	Bhi12G000365	Wax gourd	cgywgoB604

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.302200	Silver-seed gourd	carcgyB0988
Cucsa.302200	Silver-seed gourd	carcgyB1043
Cucsa.302200	Cucumber (Chinese Long) v3	cgycucB465
Cucsa.302200	Cucumber (Chinese Long) v3	cgycucB466
Cucsa.302200	Cucumber (Chinese Long) v3	cgycucB470
Cucsa.302200	Watermelon (97103) v2	cgywmbB520
Cucsa.302200	Watermelon (97103) v2	cgywmbB521
Cucsa.302200	Watermelon (97103) v2	cgywmbB522
Cucsa.302200	Wax gourd	cgywgoB606
Cucsa.302200	Cucumber (Gy14) v1	cgycgyB126
Cucsa.302200	Cucumber (Gy14) v1	cgycgyB141
Cucsa.302200	Cucurbita maxima (Rimu)	cgycmaB0830
Cucsa.302200	Cucurbita maxima (Rimu)	cgycmaB0837
Cucsa.302200	Cucurbita maxima (Rimu)	cgycmaB0839
Cucsa.302200	Cucurbita moschata (Rifu)	cgycmoB0824
Cucsa.302200	Cucurbita moschata (Rifu)	cgycmoB0826
Cucsa.302200	Cucurbita moschata (Rifu)	cgycmoB0832
Cucsa.302200	Cucurbita moschata (Rifu)	cgycmoB0834
Cucsa.302200	Wild cucumber (PI 183967)	cgycpiB452
Cucsa.302200	Wild cucumber (PI 183967)	cgycpiB453
Cucsa.302200	Wild cucumber (PI 183967)	cgycpiB457
Cucsa.302200	Cucumber (Chinese Long) v2	cgycuB434
Cucsa.302200	Cucumber (Chinese Long) v2	cgycuB429
Cucsa.302200	Cucumber (Chinese Long) v2	cgycuB430
Cucsa.302200	Melon (DHL92) v3.5.1	cgymeB495
Cucsa.302200	Melon (DHL92) v3.5.1	cgymeB497
Cucsa.302200	Melon (DHL92) v3.5.1	cgymeB498
Cucsa.302200	Watermelon (Charleston Gray)	cgywcgB519
Cucsa.302200	Watermelon (Charleston Gray)	cgywcgB520
Cucsa.302200	Watermelon (Charleston Gray)	cgywcgB521
Cucsa.302200	Watermelon (97103) v1	cgywmB547
Cucsa.302200	Watermelon (97103) v1	cgywmB549
Cucsa.302200	Watermelon (97103) v1	cgywmB552
Cucsa.302200	Cucurbita pepo (Zucchini)	cgycpeB0789
Cucsa.302200	Cucurbita pepo (Zucchini)	cgycpeB0790
Cucsa.302200	Cucurbita pepo (Zucchini)	cgycpeB0792
Cucsa.302200	Cucurbita pepo (Zucchini)	cgycpeB0796
Cucsa.302200	Bottle gourd (USVL1VR-Ls)	cgylsiB487
Cucsa.302200	Bottle gourd (USVL1VR-Ls)	cgylsiB490
Cucsa.302200	Bottle gourd (USVL1VR-Ls)	cgylsiB491
Cucsa.302200	Melon (DHL92) v3.6.1	cgymedB494
Cucsa.302200	Melon (DHL92) v3.6.1	cgymedB496
Cucsa.302200	Melon (DHL92) v3.6.1	cgymedB497