Cucsa.301910 (gene) Cucumber (Gy14) v1

NameCucsa.301910
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionL-lactate dehydrogenase
Locationscaffold02951 : 134020 .. 134307 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTAGATCTCCAACACGCTGCTGTTTTTCTCCCTCGTACAAACATCAGCGCCTCCACTGATTACTCCATTACTGCTGGATCTGATATGTGTATTGTTACTGATGGTGCTCGGCAGATCGTCGGTGAATCTAGGTTGAATCTGTTGTAGAGGAATGTTGCTCTGTTTCAGAAGATCGTTCCGTCGTTGGTTCAGTTCTCTCCTAAGATGATTCTTTTGATTGTTTCGAATCTGGTGGATGTTTTGACTTATGTTGCTTGGAAACTCTCTGGTTTTCCGTCGAATCGT

mRNA sequence

ATGCTAGATCTCCAACACGCTGCTGTTTTTCTCCCTCGTACAAACATCAGCGCCTCCACTGATTACTCCATTACTGCTGGATCTGATATGTGTATTGTTACTGATGGTGCTCGGCAGATCAGGAATGTTGCTCTGTTTCAGAAGATCGTTCCGTCGTTGGTTCAGTTCTCTCCTAAGATGATTCTTTTGATTGTTTCGAATCTGGTGGATGTTTTGACTTATGTTGCTTGGAAACTCTCTGGTTTTCCGTCGAATCGT

Coding sequence (CDS)

ATGCTAGATCTCCAACACGCTGCTGTTTTTCTCCCTCGTACAAACATCAGCGCCTCCACTGATTACTCCATTACTGCTGGATCTGATATGTGTATTGTTACTGATGGTGCTCGGCAGATCAGGAATGTTGCTCTGTTTCAGAAGATCGTTCCGTCGTTGGTTCAGTTCTCTCCTAAGATGATTCTTTTGATTGTTTCGAATCTGGTGGATGTTTTGACTTATGTTGCTTGGAAACTCTCTGGTTTTCCGTCGAATCGT

Protein sequence

MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQIRNVALFQKIVPSLVQFSPKMILLIVSNLVDVLTYVAWKLSGFPSNR
BLAST of Cucsa.301910 vs. Swiss-Prot
Match: LDH_MAIZE (L-lactate dehydrogenase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 8.1e-25
Identity = 61/96 (63.54%), Postives = 71/96 (73.96%), Query Frame = 1

Query: 1   MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQI----------RNVALFQKIV 60
           MLDLQHAA FLPRT + + TD S+T GSD+ IVT GARQI          RNVALF+KIV
Sbjct: 83  MLDLQHAAAFLPRTRLVSGTDMSVTRGSDLVIVTAGARQIQGETRLDLLQRNVALFRKIV 142

Query: 61  PSLVQFSPKMILLIVSNLVDVLTYVAWKLSGFPSNR 87
           P L + S   +LL+VSN VDVLTYVAWKLSGFP++R
Sbjct: 143 PPLAEQSHDALLLVVSNPVDVLTYVAWKLSGFPASR 178

BLAST of Cucsa.301910 vs. Swiss-Prot
Match: LDHB_HORVU (L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 3.4e-23
Identity = 56/95 (58.95%), Postives = 69/95 (72.63%), Query Frame = 1

Query: 2   LDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQI----------RNVALFQKIVP 61
           LDLQHAA FLPR  I + TD ++T  SD+ +VT GARQI          RNVAL++KIVP
Sbjct: 73  LDLQHAAAFLPRVRIVSGTDAAVTKNSDLIVVTAGARQIPGETRLNLLQRNVALYRKIVP 132

Query: 62  SLVQFSPKMILLIVSNLVDVLTYVAWKLSGFPSNR 87
            + + SP  +LL+VSN VDVLTYVAWKLSGFP++R
Sbjct: 133 PVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASR 167

BLAST of Cucsa.301910 vs. Swiss-Prot
Match: LDHA_HORVU (L-lactate dehydrogenase A OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 1.7e-22
Identity = 58/95 (61.05%), Postives = 69/95 (72.63%), Query Frame = 1

Query: 2   LDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQI----------RNVALFQKIVP 61
           LDLQHAA FLPR  IS  TD ++T  SD+ IVT GARQI          RNVAL++KIVP
Sbjct: 86  LDLQHAAAFLPRVRISG-TDAAVTKNSDLVIVTAGARQIPGETRLNLLQRNVALYRKIVP 145

Query: 62  SLVQFSPKMILLIVSNLVDVLTYVAWKLSGFPSNR 87
            + + SP  +LL+VSN VDVLTYVAWKLSGFP++R
Sbjct: 146 PVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASR 179

BLAST of Cucsa.301910 vs. Swiss-Prot
Match: LDH_CAEEL (L-lactate dehydrogenase OS=Caenorhabditis elegans GN=ldh-1 PE=2 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 9.3e-21
Identity = 51/96 (53.12%), Postives = 62/96 (64.58%), Query Frame = 1

Query: 1   MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQI----------RNVALFQKIV 60
           M+DLQH   F     + A TDYSITAGS +C+VT GARQ           RNV +F+ I+
Sbjct: 62  MMDLQHGLAFTRHCTVKADTDYSITAGSKLCVVTAGARQREGETRLSLVQRNVEIFKGII 121

Query: 61  PSLVQFSPKMILLIVSNLVDVLTYVAWKLSGFPSNR 87
           P LV++SP   +L+VSN VDVLTYV WKLSG P  R
Sbjct: 122 PQLVKYSPDTCILVVSNPVDVLTYVTWKLSGLPRER 157

BLAST of Cucsa.301910 vs. Swiss-Prot
Match: LDHA_RAT (L-lactate dehydrogenase A chain OS=Rattus norvegicus GN=Ldha PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 1.6e-20
Identity = 51/96 (53.12%), Postives = 68/96 (70.83%), Query Frame = 1

Query: 1   MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQI----------RNVALFQKIV 60
           M+DLQH ++FL    I +S DYS+TA S + I+T GARQ           RNV +F+ I+
Sbjct: 62  MMDLQHGSLFLKTPKIVSSKDYSVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFII 121

Query: 61  PSLVQFSPKMILLIVSNLVDVLTYVAWKLSGFPSNR 87
           P++V++SP+  LLIVSN VD+LTYVAWK+SGFP NR
Sbjct: 122 PNVVKYSPQCKLLIVSNPVDILTYVAWKISGFPKNR 157

BLAST of Cucsa.301910 vs. TrEMBL
Match: A0A0A0KP88_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G161910 PE=4 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 1.4e-39
Identity = 86/86 (100.00%), Postives = 86/86 (100.00%), Query Frame = 1

Query: 1   MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQIRNVALFQKIVPSLVQFSPKM 60
           MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQIRNVALFQKIVPSLVQFSPKM
Sbjct: 60  MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQIRNVALFQKIVPSLVQFSPKM 119

Query: 61  ILLIVSNLVDVLTYVAWKLSGFPSNR 87
           ILLIVSNLVDVLTYVAWKLSGFPSNR
Sbjct: 120 ILLIVSNLVDVLTYVAWKLSGFPSNR 145

BLAST of Cucsa.301910 vs. TrEMBL
Match: A0A0A0KKM5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G161940 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 3.7e-32
Identity = 77/96 (80.21%), Postives = 80/96 (83.33%), Query Frame = 1

Query: 1   MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQI----------RNVALFQKIV 60
           MLDLQHAA FLPRT ISASTDYSITAGSD+CIVT GARQI          RNVALFQKIV
Sbjct: 79  MLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAGESRLNLLQRNVALFQKIV 138

Query: 61  PSLVQFSPKMILLIVSNLVDVLTYVAWKLSGFPSNR 87
           P LVQFSP+ ILLIVSN VDVLTY+AWKLSGFPSNR
Sbjct: 139 PPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNR 174

BLAST of Cucsa.301910 vs. TrEMBL
Match: A0A0L9UPX2_PHAAN (L-lactate dehydrogenase OS=Phaseolus angularis GN=LR48_Vigan06g009900 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 5.9e-30
Identity = 72/96 (75.00%), Postives = 77/96 (80.21%), Query Frame = 1

Query: 1   MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQI----------RNVALFQKIV 60
           MLDLQHAA FLPRT I ASTDYS+TAGSD+CIVT GARQI          RNV LFQKI+
Sbjct: 54  MLDLQHAAAFLPRTRIQASTDYSVTAGSDLCIVTAGARQIHGESRLNLLQRNVTLFQKII 113

Query: 61  PSLVQFSPKMILLIVSNLVDVLTYVAWKLSGFPSNR 87
           P LV++SP  ILLIVSN VDVLTYVAWKLSGFPSNR
Sbjct: 114 PPLVRYSPDTILLIVSNPVDVLTYVAWKLSGFPSNR 149

BLAST of Cucsa.301910 vs. TrEMBL
Match: A0A0S3T5G2_PHAAN (L-lactate dehydrogenase OS=Vigna angularis var. angularis GN=Vigan.10G205800 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 5.9e-30
Identity = 72/96 (75.00%), Postives = 77/96 (80.21%), Query Frame = 1

Query: 1   MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQI----------RNVALFQKIV 60
           MLDLQHAA FLPRT I ASTDYS+TAGSD+CIVT GARQI          RNV LFQKI+
Sbjct: 81  MLDLQHAAAFLPRTRIQASTDYSVTAGSDLCIVTAGARQIHGESRLNLLQRNVTLFQKII 140

Query: 61  PSLVQFSPKMILLIVSNLVDVLTYVAWKLSGFPSNR 87
           P LV++SP  ILLIVSN VDVLTYVAWKLSGFPSNR
Sbjct: 141 PPLVRYSPDTILLIVSNPVDVLTYVAWKLSGFPSNR 176

BLAST of Cucsa.301910 vs. TrEMBL
Match: A0A0A0KKU9_CUCSA (L-lactate dehydrogenase OS=Cucumis sativus GN=Csa_5G161920 PE=3 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 1.0e-29
Identity = 75/96 (78.12%), Postives = 77/96 (80.21%), Query Frame = 1

Query: 1   MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQI----------RNVALFQKIV 60
           MLDLQHAA FLPRT ISASTDYSITA SD+CIVT GARQI          RNV LFQKIV
Sbjct: 141 MLDLQHAAAFLPRTKISASTDYSITARSDLCIVTAGARQIAGESRLNLLQRNVTLFQKIV 200

Query: 61  PSLVQFSPKMILLIVSNLVDVLTYVAWKLSGFPSNR 87
           P LVQFSP+ ILLIVSN VDVLTYVAWKLSGF SNR
Sbjct: 201 PLLVQFSPETILLIVSNPVDVLTYVAWKLSGFSSNR 236

BLAST of Cucsa.301910 vs. TAIR10
Match: AT4G17260.1 (AT4G17260.1 Lactate/malate dehydrogenase family protein)

HSP 1 Score: 123.2 bits (308), Expect = 7.6e-29
Identity = 65/96 (67.71%), Postives = 72/96 (75.00%), Query Frame = 1

Query: 1   MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQI----------RNVALFQKIV 60
           MLDLQHAA FLPRT I+AS DY +TAGSD+CIVT GARQ           RNVALF+ I+
Sbjct: 82  MLDLQHAAAFLPRTKITASVDYEVTAGSDLCIVTAGARQNPGESRLNLLQRNVALFRHII 141

Query: 61  PSLVQFSPKMILLIVSNLVDVLTYVAWKLSGFPSNR 87
           P L + SP  IL+IVSN VDVLTYVAWKLSGFP NR
Sbjct: 142 PPLAKASPDSILIIVSNPVDVLTYVAWKLSGFPVNR 177

BLAST of Cucsa.301910 vs. NCBI nr
Match: gi|700195065|gb|KGN50242.1| (hypothetical protein Csa_5G161910 [Cucumis sativus])

HSP 1 Score: 169.9 bits (429), Expect = 2.0e-39
Identity = 86/86 (100.00%), Postives = 86/86 (100.00%), Query Frame = 1

Query: 1   MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQIRNVALFQKIVPSLVQFSPKM 60
           MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQIRNVALFQKIVPSLVQFSPKM
Sbjct: 60  MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQIRNVALFQKIVPSLVQFSPKM 119

Query: 61  ILLIVSNLVDVLTYVAWKLSGFPSNR 87
           ILLIVSNLVDVLTYVAWKLSGFPSNR
Sbjct: 120 ILLIVSNLVDVLTYVAWKLSGFPSNR 145

BLAST of Cucsa.301910 vs. NCBI nr
Match: gi|778700018|ref|XP_004151663.2| (PREDICTED: LOW QUALITY PROTEIN: L-lactate dehydrogenase A-like [Cucumis sativus])

HSP 1 Score: 145.2 bits (365), Expect = 5.3e-32
Identity = 77/96 (80.21%), Postives = 80/96 (83.33%), Query Frame = 1

Query: 1   MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQI----------RNVALFQKIV 60
           MLDLQHAA FLPRT ISASTDYSITAGSD+CIVT GARQI          RNVALFQKIV
Sbjct: 79  MLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAGESRLNLLQRNVALFQKIV 138

Query: 61  PSLVQFSPKMILLIVSNLVDVLTYVAWKLSGFPSNR 87
           P LVQFSP+ ILLIVSN VDVLTY+AWKLSGFPSNR
Sbjct: 139 PPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNR 174

BLAST of Cucsa.301910 vs. NCBI nr
Match: gi|700195068|gb|KGN50245.1| (hypothetical protein Csa_5G161940 [Cucumis sativus])

HSP 1 Score: 145.2 bits (365), Expect = 5.3e-32
Identity = 77/96 (80.21%), Postives = 80/96 (83.33%), Query Frame = 1

Query: 1   MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQI----------RNVALFQKIV 60
           MLDLQHAA FLPRT ISASTDYSITAGSD+CIVT GARQI          RNVALFQKIV
Sbjct: 79  MLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAGESRLNLLQRNVALFQKIV 138

Query: 61  PSLVQFSPKMILLIVSNLVDVLTYVAWKLSGFPSNR 87
           P LVQFSP+ ILLIVSN VDVLTY+AWKLSGFPSNR
Sbjct: 139 PPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNR 174

BLAST of Cucsa.301910 vs. NCBI nr
Match: gi|659073513|ref|XP_008437100.1| (PREDICTED: L-lactate dehydrogenase B [Cucumis melo])

HSP 1 Score: 141.4 bits (355), Expect = 7.6e-31
Identity = 76/96 (79.17%), Postives = 79/96 (82.29%), Query Frame = 1

Query: 1   MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQI----------RNVALFQKIV 60
           MLDLQHAA FLPRT ISASTDYS TAGS++CIVT GARQI          RNVALFQKIV
Sbjct: 161 MLDLQHAAAFLPRTKISASTDYSTTAGSNLCIVTAGARQIAGESRLNLLQRNVALFQKIV 220

Query: 61  PSLVQFSPKMILLIVSNLVDVLTYVAWKLSGFPSNR 87
           P LVQFSP+ ILLIVSN VDVLTYVAWKLSGFPSNR
Sbjct: 221 PPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNR 256

BLAST of Cucsa.301910 vs. NCBI nr
Match: gi|951047968|ref|XP_014519575.1| (PREDICTED: L-lactate dehydrogenase A-like [Vigna radiata var. radiata])

HSP 1 Score: 138.3 bits (347), Expect = 6.4e-30
Identity = 73/96 (76.04%), Postives = 77/96 (80.21%), Query Frame = 1

Query: 1   MLDLQHAAVFLPRTNISASTDYSITAGSDMCIVTDGARQI----------RNVALFQKIV 60
           MLDLQHAA FLPRT I ASTDYSITAGSD+CIVT GARQI          RNV LFQKI+
Sbjct: 79  MLDLQHAAAFLPRTRIQASTDYSITAGSDLCIVTAGARQIHGESRLNLLQRNVTLFQKII 138

Query: 61  PSLVQFSPKMILLIVSNLVDVLTYVAWKLSGFPSNR 87
           P LV++SP  ILLIVSN VDVLTYVAWKLSGFPSNR
Sbjct: 139 PPLVRYSPDTILLIVSNPVDVLTYVAWKLSGFPSNR 174

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
LDH_MAIZE8.1e-2563.54L-lactate dehydrogenase OS=Zea mays PE=2 SV=1[more]
LDHB_HORVU3.4e-2358.95L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare PE=1 SV=1[more]
LDHA_HORVU1.7e-2261.05L-lactate dehydrogenase A OS=Hordeum vulgare PE=1 SV=1[more]
LDH_CAEEL9.3e-2153.13L-lactate dehydrogenase OS=Caenorhabditis elegans GN=ldh-1 PE=2 SV=1[more]
LDHA_RAT1.6e-2053.13L-lactate dehydrogenase A chain OS=Rattus norvegicus GN=Ldha PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KP88_CUCSA1.4e-39100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G161910 PE=4 SV=1[more]
A0A0A0KKM5_CUCSA3.7e-3280.21Uncharacterized protein OS=Cucumis sativus GN=Csa_5G161940 PE=4 SV=1[more]
A0A0L9UPX2_PHAAN5.9e-3075.00L-lactate dehydrogenase OS=Phaseolus angularis GN=LR48_Vigan06g009900 PE=3 SV=1[more]
A0A0S3T5G2_PHAAN5.9e-3075.00L-lactate dehydrogenase OS=Vigna angularis var. angularis GN=Vigan.10G205800 PE=... [more]
A0A0A0KKU9_CUCSA1.0e-2978.13L-lactate dehydrogenase OS=Cucumis sativus GN=Csa_5G161920 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G17260.17.6e-2967.71 Lactate/malate dehydrogenase family protein[more]
Match NameE-valueIdentityDescription
gi|700195065|gb|KGN50242.1|2.0e-39100.00hypothetical protein Csa_5G161910 [Cucumis sativus][more]
gi|778700018|ref|XP_004151663.2|5.3e-3280.21PREDICTED: LOW QUALITY PROTEIN: L-lactate dehydrogenase A-like [Cucumis sativus][more]
gi|700195068|gb|KGN50245.1|5.3e-3280.21hypothetical protein Csa_5G161940 [Cucumis sativus][more]
gi|659073513|ref|XP_008437100.1|7.6e-3179.17PREDICTED: L-lactate dehydrogenase B [Cucumis melo][more]
gi|951047968|ref|XP_014519575.1|6.4e-3076.04PREDICTED: L-lactate dehydrogenase A-like [Vigna radiata var. radiata][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001236Lactate/malate_DH_N
IPR001557L-lactate/malate_DH
IPR016040NAD(P)-bd_dom
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
GO:0016616oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO:0019752carboxylic acid metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006534 cysteine metabolic process
biological_process GO:0006094 gluconeogenesis
biological_process GO:0006096 glycolytic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0019752 carboxylic acid metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004459 L-lactate dehydrogenase activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.301910.1Cucsa.301910.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001236Lactate/malate dehydrogenase, N-terminalPFAMPF00056Ldh_1_Ncoord: 1..86
score: 1.0
IPR001557L-lactate/malate dehydrogenasePANTHERPTHR11540MALATE AND LACTATE DEHYDROGENASEcoord: 1..86
score: 1.5
IPR016040NAD(P)-binding domainGENE3DG3DSA:3.40.50.720coord: 1..86
score: 6.1
IPR016040NAD(P)-binding domainunknownSSF51735NAD(P)-binding Rossmann-fold domainscoord: 1..86
score: 8.45
NoneNo IPR availablePANTHERPTHR11540:SF3L-LACTATE DEHYDROGENASEcoord: 1..86
score: 1.5

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None