Cucsa.185460 (gene) Cucumber (Gy14) v1

NameCucsa.185460
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionRibose-phosphate pyrophosphokinase, putative
Locationscaffold01274 : 943684 .. 947079 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGTTTCCTCTCTCTCTCTCTCTCTTTCTGCTTTGAGTGAAAGCAGCTTTGTGGTGGAAGTTGAAGTTGTCGGAATCAATGGCAAAGCCGCCGAAGAAGCACATCTTTCTCTTTTACTGCGAGGAATGCGAAGAACTTGCTCAAAAAGTTGCAGCTCAATCCGATTCCATCACTTTACAAACCATCAAATGGAGGTCCATTCCTTTTCCTCTTCCCTTAAATGCAAATTTCTTTGTTTCCCCTTAGATAAATGTTTGTATTTCTAAGTTTTCTGGCTTTTCTTTTGGTTTTCAGTTCTTAACTGTAGTTTTTTATTAGCTTGTTAATGTAATGATCATCTTGTGCTTGAAAATCTACAAACTTTAAGTAGACTGAGATAAAAAGTTTAAGAACCCATTTGGTTCGATTAGTTTTTGTTATGAACAAAACTTTTCCAGAACAAATAGCTGGTGGTGATCCTTTGGGTTCTTAGTTTTTAAAGTTGAGCTTGTTGTCCTTTCCTGTGAAATTGTTTTGCCCCTGTTCTACTTCTAAAATGTAAGTTTCTCTGCTGGTTTGTTTGACAGACATTACTGTATAGTTGTTACAAAAGAAAAAGGTATTTAGACATTTTATATGTTATAGATCTATATGAGACACATTTAGTAAAGAAAATGGGAAAAAAGTGATGAAGCTATGAACAACTCTAAAACTCAAAATGAATCCAAGAGTTGCAAGGGAGTGAAATTTCTTTACCTACCCATGTACCTATCTGAGCCAAGAAGATAACTAAAAGTTCACGAGACTGTAAATTGGTTATCTTTTTGTCTACCTATCGGCTATCTACTATAATCTGTCTGTAGACCTCGTATCTATGTTTATCTATGTTTTTCTAATCTTGAGGAGCAAATACCACTAGCAATCGACTGCATTTTAGTCCTTCATCATCTAATTACCTATATGCCAGAGAGTGGTAGATGAGGCTTCCTAACTCGAAAAGAAAAGTAGAGTGAAGAAGTACGGTTTCACGACTTGGGAATCAAAATGAACATATTAATGTATTAGCCATGTCCTGATTCTGTTGAAGCTGGGATTTTCCAATTCTCTGCTGCCATATCTGTGCCCTTTAATTGTATCCTTTCTTCTTGGGTTCTCTTTGCATGCCTTTGCTGCTGCTAAGAATTTGCTTTGTAGTTCATTTTGGTTTTTGTTAGTATGTCAAGTGTCAACTATACTGCTGCTTGTAAACTGCTTGAGGTAGTCTTTCATTTGGTAAGTACTATAATAAGTTCACACTATATACAGGACATTTGATGATGGTTTTCCAAATATATACATAAACAATGCTCAAGAACTGCGTGGGAATCATGTAGCATTCCTTGCATCTTTCAGCCACCAAGGAGCTATATTTGAACAGATATCAGTCATATACAATCTCCCAAGGCTTTTCGCTGCTTCATTCACGTTGGTATTGCCTTTCTTTCCTACTGGCTCCTTTGAGCGAATGGAAGAAGAAGGGGATGTAGCAACAGCATTTACGATGGCAAGAATCCTATCAAATATTCCTATTTCAAGAGGTGGCCCAACAAGCGTAGTAATCTATGATATACATGCCTTGCAGGTTTGTTGTTATTGATTGAATTTATGTTCTAAAACTAAGCCTTCCATTGCTGATGATTAAATCACTGGATAGCGTAGGAAGCAGCACTGGACATGGCCTCTTGCTAGTTAGTGTCATGTCAGTTGTTGATTTTGCGCTACCGTAGCTAGCTACAGCTGTTCTTATATTGATCATCTTCGCATGAATTAACTTTTACCAGTTGCTTTGTAACCTGGGAGAATCCTTTGACATCTTCACTCTCTTAGAATTTATCTTCCCCTTGTTGCCAATGTGGTTATATTGGAACGATATCATCTTTTATATTGATCTCCCTTTCACTACAGGAAAGATTTTACTTTGGGGATAATGTTTTGCCTTTGTTTGAAACGGGAATTCCACTCCTGAAGCAACGTCTACACCAACTTCCTGATGCTGACGATGTGAGTATGCTGTACAATGCCCAAAAGACTTTCTTTCAAACCATTTTTCCCCCTTTTACATTGTTCATGATTTAACCATGCTATTGTGCAGGTAGTAATAGCATTTCCTGATGATGGAGCTTGGAAGCGATTCCACAAGCTACTTGATCGTTATCCTGTGGTTAGTTGGAGTTTATCATATCTTCAAAGTACAGTCTCGTTTATGAGTACATTTTTCTCAGCATTCTAAAATATGATGCTGACTAATGGCAGGTCATCTGTACGAAAGTTCGTGAAGAAGACAAAAGGATAGTGCGAATCAAGGAAGGAAATCCTGCTGGTCGGCATGTGGTTATTGTTGATGATTTGGTACAATCTGGAGGAACCTTGATTGAGTGTCAGGTATTCCTCTCCTCTGGCACCTAACCCTGCTAGATACACTTGTGAACCACTGTCTCAGATATCATTTGAAGTCAATGCTCCATCACTTATTTGGTTTGGTACCGGAACTGCTGCATACTAATTGTGTTTTTAATCGGCTGTGAGCTGCAAAAAATTCCTTTCACTTGAACATCATACTTTTTATGCCACAATATAATTTCTGCACACAATTCTAAATTTCTAAGAAGCGTGCCCATTTTCCAGATTGATTCTTTTTCAAAAGTTTGTGATCAAGATGTATGCTGACAACCTCCAATCATATTCTTGGCTCTTGCTTGTCTGCTGCATTCTAATTTTTCTGCTCATTTTACTATCAGAAAGTTTTAGCAGCCCACGGAGCGGCCAAGGTTAGTGCATATGTTACTCATGGTGTTTTCCCAAAGCAATCATGGAAGCGGTTCCTTCACAAAAATGGTGAGTGGTTGAAGTTTTAGGAGTGAGCTTAAAATCACTTGTATCTAACTCAAAATCACTTTAGTATATCTTTAGTCTTTCAAAATTAAATTAATGTTTAGTTTCATACTTTGCACTTCCATACAAACAAAATTGATTAAAAAATTTTAAAAACATGTTTCAAACTAATTTTGAACTTAACAAAAACAATTTAACAATCACTACCAAAAATATCAATAATCATTCAAAGTAGGGTAATCTATATCTTGATATTCTTTCTGAAGGTTTGGAGATGGCATTTTCCTACTTCTGGATCACTGATTCCTGCCCTCATACAGTCAAAGCCATAGCGGATAAAGCTCCTTTTGAAGTTTTGAGTCTTGCAGGTTCCATTGCTGATGCTTTGGTGATTTGAATCGATAGGTTATTGATGAGACTTAAATTATGCAAATGTGACACATATTGTTAACAATAATGCATAGGGGGGACACAGTTCAGATTCTGACATATGAGTGAATGATGGATGACATCCTTTCTTATGAAATTGATGGTTTTTAACAATA

mRNA sequence

tttgtttcctctctctctctctctctttctGCTTTGAGTGAAAGCAGCTTTGTGGTGGAAGTTGAAGTTGTCGGAATCAATGGCAAAGCCGCCGAAGAAGCACATCTTTCTCTTTTACTGCGAGGAATGCGAAGAACTTGCTCAAAAAGTTGCAGCTCAATCCGATTCCATCACTTTACAAACCATCAAATGGAGGACATTTGATGATGGTTTTCCAAATATATACATAAACAATGCTCAAGAACTGCGTGGGAATCATGTAGCATTCCTTGCATCTTTCAGCCACCAAGGAGCTATATTTGAACAGATATCAGTCATATACAATCTCCCAAGGCTTTTCGCTGCTTCATTCACGTTGGTATTGCCTTTCTTTCCTACTGGCTCCTTTGAGCGAATGGAAGAAGAAGGGGATGTAGCAACAGCATTTACGATGGCAAGAATCCTATCAAATATTCCTATTTCAAGAGGTGGCCCAACAAGCGTAGTAATCTATGATATACATGCCTTGCAGGAAAGATTTTACTTTGGGGATAATGTTTTGCCTTTGTTTGAAACGGGAATTCCACTCCTGAAGCAACGTCTACACCAACTTCCTGATGCTGACGATGTAGTAATAGCATTTCCTGATGATGGAGCTTGGAAGCGATTCCACAAGCTACTTGATCGTTATCCTGTGGTCATCTGTACGAAAGTTCGTGAAGAAGACAAAAGGATAGTGCGAATCAAGGAAGGAAATCCTGCTGGTCGGCATGTGGTTATTGTTGATGATTTGGTACAATCTGGAGGAACCTTGATTGAGTGTCAGAAAGTTTTAGCAGCCCACGGAGCGGCCAAGGTTAGTGCATATGTTACTCATGGTGTTTTCCCAAAGCAATCATGGAAGCGGTTCCTTCACAAAAATGGTTTGGAGATGGCATTTTCCTACTTCTGGATCACTGATTCCTGCCCTCATACAGTCAAAGCCATAGCGGATAAAGCTCCTTTTGAAGTTTTGAGTCTTGCAGGTTCCATTGCTGATGCTTTGGTGATTTGAATCGATAGGTTATTGATGAGACTTAAATTATGCAAATGTGACACATATTGTTAACAATAATGCATAGGGGGGACACAGTTCAGATTCTGACATATGAGTGAATGATGGATGACATCCTTTCTTATGAAATTGATGGTTTTTAACAATA

Coding sequence (CDS)

ATGGCAAAGCCGCCGAAGAAGCACATCTTTCTCTTTTACTGCGAGGAATGCGAAGAACTTGCTCAAAAAGTTGCAGCTCAATCCGATTCCATCACTTTACAAACCATCAAATGGAGGACATTTGATGATGGTTTTCCAAATATATACATAAACAATGCTCAAGAACTGCGTGGGAATCATGTAGCATTCCTTGCATCTTTCAGCCACCAAGGAGCTATATTTGAACAGATATCAGTCATATACAATCTCCCAAGGCTTTTCGCTGCTTCATTCACGTTGGTATTGCCTTTCTTTCCTACTGGCTCCTTTGAGCGAATGGAAGAAGAAGGGGATGTAGCAACAGCATTTACGATGGCAAGAATCCTATCAAATATTCCTATTTCAAGAGGTGGCCCAACAAGCGTAGTAATCTATGATATACATGCCTTGCAGGAAAGATTTTACTTTGGGGATAATGTTTTGCCTTTGTTTGAAACGGGAATTCCACTCCTGAAGCAACGTCTACACCAACTTCCTGATGCTGACGATGTAGTAATAGCATTTCCTGATGATGGAGCTTGGAAGCGATTCCACAAGCTACTTGATCGTTATCCTGTGGTCATCTGTACGAAAGTTCGTGAAGAAGACAAAAGGATAGTGCGAATCAAGGAAGGAAATCCTGCTGGTCGGCATGTGGTTATTGTTGATGATTTGGTACAATCTGGAGGAACCTTGATTGAGTGTCAGAAAGTTTTAGCAGCCCACGGAGCGGCCAAGGTTAGTGCATATGTTACTCATGGTGTTTTCCCAAAGCAATCATGGAAGCGGTTCCTTCACAAAAATGGTTTGGAGATGGCATTTTCCTACTTCTGGATCACTGATTCCTGCCCTCATACAGTCAAAGCCATAGCGGATAAAGCTCCTTTTGAAGTTTTGAGTCTTGCAGGTTCCATTGCTGATGCTTTGGTGATTTGA

Protein sequence

MAKPPKKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNHVAFLASFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMARILSNIPISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIAFPDDGAWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIECQKVLAAHGAAKVSAYVTHGVFPKQSWKRFLHKNGLEMAFSYFWITDSCPHTVKAIADKAPFEVLSLAGSIADALVI*
BLAST of Cucsa.185460 vs. Swiss-Prot
Match: KPRS4_ARATH (Ribose-phosphate pyrophosphokinase 4 OS=Arabidopsis thaliana GN=PRS4 PE=1 SV=2)

HSP 1 Score: 528.9 bits (1361), Expect = 3.9e-149
Identity = 259/313 (82.75%), Postives = 280/313 (89.46%), Query Frame = 1

Query: 6   KKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNHVAFLA 65
           KK + LFYC ECEELA+ +AA+SD ITLQ+I WR+F DGFPN++INNA ++RG HVAFLA
Sbjct: 25  KKKVHLFYCLECEELARNIAAESDHITLQSINWRSFADGFPNLFINNAHDIRGQHVAFLA 84

Query: 66  SFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMARILSNI 125
           SFS    IFEQISVIY LPRLF ASFTLVLPFFPTGSFERMEEEGDVATAFTMARI+SNI
Sbjct: 85  SFSSPAVIFEQISVIYLLPRLFVASFTLVLPFFPTGSFERMEEEGDVATAFTMARIVSNI 144

Query: 126 PISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIAFPDDG 185
           PISRGGPTSVVIYDIHALQERFYF D VLPLFETGIPLL +RL QLP+ + V++AFPDDG
Sbjct: 145 PISRGGPTSVVIYDIHALQERFYFADQVLPLFETGIPLLTKRLQQLPETEKVIVAFPDDG 204

Query: 186 AWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIECQKVL 245
           AWKRFHKLLD YP V+CTKVRE DKRIVR+KEGNPAG HVVIVDDLVQSGGTLIECQKVL
Sbjct: 205 AWKRFHKLLDHYPTVVCTKVREGDKRIVRLKEGNPAGCHVVIVDDLVQSGGTLIECQKVL 264

Query: 246 AAHGAAKVSAYVTHGVFPKQSWKRFLH-KNGLEMAFSYFWITDSCPHTVKAIADKAPFEV 305
           AAHGA KVSAYVTHGVFPK SW+RF H KNGLE AF+YFWITDSCP TVKAI +KAPFEV
Sbjct: 265 AAHGAVKVSAYVTHGVFPKSSWERFTHKKNGLEEAFAYFWITDSCPQTVKAIGNKAPFEV 324

Query: 306 LSLAGSIADALVI 318
           LSLAGSIADAL I
Sbjct: 325 LSLAGSIADALQI 337

BLAST of Cucsa.185460 vs. Swiss-Prot
Match: KPRS4_SPIOL (Ribose-phosphate pyrophosphokinase 4 OS=Spinacia oleracea GN=PRS4 PE=2 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 5.1e-149
Identity = 260/318 (81.76%), Postives = 284/318 (89.31%), Query Frame = 1

Query: 1   MAKPPKKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNH 60
           M KP  K + LFYC E EELA+KVAAQS  ITLQ+I WR+FDDGFPN++INNAQ++RG H
Sbjct: 1   MEKPNTKQVLLFYCVEAEELARKVAAQSPLITLQSINWRSFDDGFPNLFINNAQDIRGQH 60

Query: 61  VAFLASFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMAR 120
           VAFLA+FS    IFEQ+SVIY LPRLF ASFTLVLPFFPTGSFERMEEEGDVATAFTMAR
Sbjct: 61  VAFLAAFSSPAVIFEQLSVIYALPRLFVASFTLVLPFFPTGSFERMEEEGDVATAFTMAR 120

Query: 121 ILSNIPISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIA 180
           ILSNIP+SRGGPTSVVIYDIHALQERFYF DNVLPLFETGIPLLKQRL QLPDAD +V+A
Sbjct: 121 ILSNIPVSRGGPTSVVIYDIHALQERFYFSDNVLPLFETGIPLLKQRLDQLPDADKIVVA 180

Query: 181 FPDDGAWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIE 240
           FPDDGAWKRFHK LD +P+V+C KVRE DKRIVR+KEGNPAG HVVIVDDLVQSGGTLIE
Sbjct: 181 FPDDGAWKRFHKQLDHFPMVVCAKVREGDKRIVRLKEGNPAGCHVVIVDDLVQSGGTLIE 240

Query: 241 CQKVLAAHGAAKVSAYVTHGVFPKQSWKRFLHK-NGLEMAFSYFWITDSCPHTVKAIADK 300
           CQKVLAAHGA KVSAYVTH VFPK S++RF HK +G + AF+YFWITDSCP TVK+IA+K
Sbjct: 241 CQKVLAAHGATKVSAYVTHAVFPKNSFERFTHKDDGSDKAFTYFWITDSCPRTVKSIANK 300

Query: 301 APFEVLSLAGSIADALVI 318
           APFEVLSLAGSIADAL I
Sbjct: 301 APFEVLSLAGSIADALQI 318

BLAST of Cucsa.185460 vs. Swiss-Prot
Match: KPRS4_ORYSJ (Ribose-phosphate pyrophosphokinase 4 OS=Oryza sativa subsp. japonica GN=Os02g0714600 PE=2 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 1.3e-141
Identity = 252/314 (80.25%), Postives = 273/314 (86.94%), Query Frame = 1

Query: 6   KKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNHVAFLA 65
           KK I LFYC ECEELA KVAA SD+I LQ+I WR+FDDGFPN++INNA ++RG HVAFLA
Sbjct: 12  KKQINLFYCSECEELALKVAASSDTIHLQSINWRSFDDGFPNLFINNAHDIRGQHVAFLA 71

Query: 66  SFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMARILSNI 125
           SFS    IFEQISVI+ LP+LF ASFTLVLPFFPTGSFER+EEEGDVATAFT+ARILS I
Sbjct: 72  SFSSPSVIFEQISVIFALPKLFIASFTLVLPFFPTGSFERVEEEGDVATAFTLARILSMI 131

Query: 126 PISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIAFPDDG 185
           P SRGGPTSVVIYDIHALQERFYFGD+VLP FETGIPLL QRL QLPDAD++ IAFPDDG
Sbjct: 132 PKSRGGPTSVVIYDIHALQERFYFGDDVLPCFETGIPLLLQRLRQLPDADNITIAFPDDG 191

Query: 186 AWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIECQKVL 245
           AWKRFHKLL  +P+V+C KVRE DKRIVRIKEGNP GRHVVIVDDLVQSGGTL ECQKVL
Sbjct: 192 AWKRFHKLLLNFPMVVCAKVREGDKRIVRIKEGNPEGRHVVIVDDLVQSGGTLRECQKVL 251

Query: 246 AAHGAAKVSAYVTHGVFPKQSWKRFLHKNGLEMA--FSYFWITDSCPHTVKAIADKAPFE 305
           AAHGAAKVSAYVTH VFPKQS++RF H N    A  F+YFWITDSCP TVKAI  + PFE
Sbjct: 252 AAHGAAKVSAYVTHAVFPKQSYERFTHTNSAGSADKFAYFWITDSCPQTVKAINQQPPFE 311

Query: 306 VLSLAGSIADALVI 318
           VLSLAGSIADAL I
Sbjct: 312 VLSLAGSIADALQI 325

BLAST of Cucsa.185460 vs. Swiss-Prot
Match: KPRS3_SPIOL (Ribose-phosphate pyrophosphokinase 3, mitochondrial OS=Spinacia oleracea GN=PRS3 PE=2 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 2.8e-131
Identity = 225/313 (71.88%), Postives = 268/313 (85.62%), Query Frame = 1

Query: 7   KHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNHVAFLAS 66
           K + LFYC E + LA+++AAQSD+I L++I WRTF+DGFPN++I+NAQ +RG HVAFLAS
Sbjct: 94  KKVCLFYCPETKALAERIAAQSDAIQLRSISWRTFEDGFPNLFISNAQGIRGKHVAFLAS 153

Query: 67  FSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMARILSNIP 126
           FS  G IFEQ+SVIY LP+LF ASF LVLPFFPTG+ ERME+EGDVATAFT+ARILSNIP
Sbjct: 154 FSSPGVIFEQLSVIYALPKLFVASFKLVLPFFPTGTSERMEDEGDVATAFTLARILSNIP 213

Query: 127 ISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIAFPDDGA 186
           ISR GPTS+V +DIHALQERFYFGDN+LP FE+GIPLLK++L QLPD+D++ IAFPDDGA
Sbjct: 214 ISREGPTSLVTFDIHALQERFYFGDNILPCFESGIPLLKKKLQQLPDSDNITIAFPDDGA 273

Query: 187 WKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIECQKVLA 246
           WKRFHK L  +P+++C KVRE D+RIVR+KEG+P GRHVVIVDDLVQSGGTLIECQKVLA
Sbjct: 274 WKRFHKQLQHFPMIVCAKVREGDQRIVRLKEGDPTGRHVVIVDDLVQSGGTLIECQKVLA 333

Query: 247 AHGAAKVSAYVTHGVFPKQSWKRFLHKNG--LEMAFSYFWITDSCPHTVKAIADKAPFEV 306
           AHGAAKVSAYVTHG+FP +SW+RF        E   ++FWITDSCP TVK + ++ PFEV
Sbjct: 334 AHGAAKVSAYVTHGIFPNKSWERFKPDTAGCPEEGMTHFWITDSCPLTVKMVKNRPPFEV 393

Query: 307 LSLAGSIADALVI 318
           +SLAGSIA AL I
Sbjct: 394 ISLAGSIAAALQI 406

BLAST of Cucsa.185460 vs. Swiss-Prot
Match: KPRS3_ORYSJ (Ribose-phosphate pyrophosphokinase 3, chloroplastic OS=Oryza sativa subsp. japonica GN=Os01g0723600 PE=2 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 2.6e-129
Identity = 221/313 (70.61%), Postives = 266/313 (84.98%), Query Frame = 1

Query: 7   KHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNHVAFLAS 66
           K++ LF+CEE  ELA++V A++D I L++I WRTF DGFPN++I+NA  +RG HVAFLAS
Sbjct: 97  KNVLLFHCEEMRELAEQVVARNDDIELRSISWRTFADGFPNLFISNAHTIRGQHVAFLAS 156

Query: 67  FSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMARILSNIP 126
           FS    IFEQ+S+IY LP+LF +SFTL+LPFFPTG+ ERME+EGDVATAFT+ARILSNIP
Sbjct: 157 FSSPSVIFEQLSIIYALPKLFISSFTLILPFFPTGTSERMEDEGDVATAFTLARILSNIP 216

Query: 127 ISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIAFPDDGA 186
           ISRGGP+S+VI+DIHALQERFYFGD+VLP FE+GIPLLK RL +LPD+D++ IAFPDDGA
Sbjct: 217 ISRGGPSSLVIFDIHALQERFYFGDSVLPCFESGIPLLKSRLQELPDSDNITIAFPDDGA 276

Query: 187 WKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIECQKVLA 246
           WKRF+K L  +P+V+C KVRE ++RIVRIKEG+P GRHVVIVDDLVQSGGTLIECQKVLA
Sbjct: 277 WKRFYKQLQHFPMVVCNKVREGEQRIVRIKEGDPRGRHVVIVDDLVQSGGTLIECQKVLA 336

Query: 247 AHGAAKVSAYVTHGVFPKQSWKRFLHKN--GLEMAFSYFWITDSCPHTVKAIADKAPFEV 306
            HGAAKVSAYVTHG+FP +SW++F   N  G     S+FWITDSCP TV A+ D+ PFE+
Sbjct: 337 EHGAAKVSAYVTHGIFPNKSWEKFQPDNGEGPGHGLSHFWITDSCPLTVNAVKDRQPFEI 396

Query: 307 LSLAGSIADALVI 318
           LSLAG IA AL I
Sbjct: 397 LSLAGPIASALQI 409

BLAST of Cucsa.185460 vs. TrEMBL
Match: A0A0A0KI24_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G423440 PE=3 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 2.1e-181
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 1

Query: 1   MAKPPKKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNH 60
           MAKPPKKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNH
Sbjct: 1   MAKPPKKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNH 60

Query: 61  VAFLASFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMAR 120
           VAFLASFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMAR
Sbjct: 61  VAFLASFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMAR 120

Query: 121 ILSNIPISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIA 180
           ILSNIPISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIA
Sbjct: 121 ILSNIPISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIA 180

Query: 181 FPDDGAWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIE 240
           FPDDGAWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIE
Sbjct: 181 FPDDGAWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIE 240

Query: 241 CQKVLAAHGAAKVSAYVTHGVFPKQSWKRFLHKNGLEMAFSYFWITDSCPHTVKAIADKA 300
           CQKVLAAHGAAKVSAYVTHGVFPKQSWKRFLHKNGLEMAFSYFWITDSCPHTVKAIADKA
Sbjct: 241 CQKVLAAHGAAKVSAYVTHGVFPKQSWKRFLHKNGLEMAFSYFWITDSCPHTVKAIADKA 300

Query: 301 PFEVLSLAGSIADALVI 318
           PFEVLSLAGSIADALVI
Sbjct: 301 PFEVLSLAGSIADALVI 317

BLAST of Cucsa.185460 vs. TrEMBL
Match: M5WT69_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008810mg PE=3 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 2.6e-155
Identity = 269/317 (84.86%), Postives = 293/317 (92.43%), Query Frame = 1

Query: 1   MAKPPKKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNH 60
           M    KK +FLFYC ECE+LA++VA+QSD ITLQTIKWRTFDDGFPNI+INNAQ+LRG H
Sbjct: 1   MENSQKKQVFLFYCAECEDLARQVASQSDHITLQTIKWRTFDDGFPNIFINNAQDLRGQH 60

Query: 61  VAFLASFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMAR 120
           VAFLASFS QG IFEQ+SVIY LPRLF ASFTLVLPFFPTGSFERMEEEGDVATAFTMAR
Sbjct: 61  VAFLASFSSQGVIFEQLSVIYALPRLFVASFTLVLPFFPTGSFERMEEEGDVATAFTMAR 120

Query: 121 ILSNIPISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIA 180
           +LSNIPIS+GGP SV+IYDIHALQERFYFGD+VLPLFETGIPLLKQRLHQLPD+D +V+A
Sbjct: 121 MLSNIPISKGGPNSVLIYDIHALQERFYFGDHVLPLFETGIPLLKQRLHQLPDSDKIVVA 180

Query: 181 FPDDGAWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIE 240
           FPDDGAWKRFHK LD +P+V+C KVRE DKRIVRIKEGNPAG HVVIVDDLVQSGGTLIE
Sbjct: 181 FPDDGAWKRFHKQLDHFPMVVCNKVREGDKRIVRIKEGNPAGYHVVIVDDLVQSGGTLIE 240

Query: 241 CQKVLAAHGAAKVSAYVTHGVFPKQSWKRFLHKNGLEMAFSYFWITDSCPHTVKAIADKA 300
           CQKVLAAHGAAKVSAYVTHGVFPK+SW+RF HK+G E AF+YFWITDSCP TVKA+A+KA
Sbjct: 241 CQKVLAAHGAAKVSAYVTHGVFPKRSWERFTHKDGSEKAFAYFWITDSCPLTVKAVANKA 300

Query: 301 PFEVLSLAGSIADALVI 318
           PFEVLSLAGSIADAL I
Sbjct: 301 PFEVLSLAGSIADALQI 317

BLAST of Cucsa.185460 vs. TrEMBL
Match: W9RT02_9ROSA (Ribose-phosphate pyrophosphokinase 4 OS=Morus notabilis GN=L484_021391 PE=4 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 2.2e-154
Identity = 266/310 (85.81%), Postives = 292/310 (94.19%), Query Frame = 1

Query: 6   KKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNHVAFLA 65
           KK ++LFYC ECE+LAQKVA+QSD ITLQTIKWR+FDDGFPNI+INNAQ LRG HVAFLA
Sbjct: 6   KKQVYLFYCSECEDLAQKVASQSDFITLQTIKWRSFDDGFPNIFINNAQVLRGQHVAFLA 65

Query: 66  SFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMARILSNI 125
           SFS QG IFEQ+SVIY LPRLF ASFTLVLPFFPTGSFERMEEEGDVATAFT+AR+LSNI
Sbjct: 66  SFSSQGVIFEQLSVIYALPRLFVASFTLVLPFFPTGSFERMEEEGDVATAFTLARMLSNI 125

Query: 126 PISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIAFPDDG 185
           PISRGGPTS+VIYDIHALQERFYFGD+VLPLFETGIPLLKQRLHQLPD+D +V+AFPDDG
Sbjct: 126 PISRGGPTSLVIYDIHALQERFYFGDHVLPLFETGIPLLKQRLHQLPDSDKIVVAFPDDG 185

Query: 186 AWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIECQKVL 245
           AWKRFHK LD +P+V+C KVRE DKRIVRIKEGNPAG HVVIVDDLVQSGGTLIECQKVL
Sbjct: 186 AWKRFHKQLDHFPMVVCNKVREGDKRIVRIKEGNPAGYHVVIVDDLVQSGGTLIECQKVL 245

Query: 246 AAHGAAKVSAYVTHGVFPKQSWKRFLHKNGLEMAFSYFWITDSCPHTVKAIADKAPFEVL 305
           AAHGA+KVSAYVTHGVFPK+SW+RF HK+GLE AF+YFWITDSCP TVKAIA++APFEVL
Sbjct: 246 AAHGASKVSAYVTHGVFPKRSWERFTHKDGLEKAFAYFWITDSCPLTVKAIANRAPFEVL 305

Query: 306 SLAGSIADAL 316
           SLAGSI++AL
Sbjct: 306 SLAGSISEAL 315

BLAST of Cucsa.185460 vs. TrEMBL
Match: M5XCT7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008810mg PE=3 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 6.3e-154
Identity = 269/318 (84.59%), Postives = 293/318 (92.14%), Query Frame = 1

Query: 1   MAKPPKKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNH 60
           M    KK +FLFYC ECE+LA++VA+QSD ITLQTIKWRTFDDGFPNI+INNAQ+LRG H
Sbjct: 1   MENSQKKQVFLFYCAECEDLARQVASQSDHITLQTIKWRTFDDGFPNIFINNAQDLRGQH 60

Query: 61  VAFLASFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMAR 120
           VAFLASFS QG IFEQ+SVIY LPRLF ASFTLVLPFFPTGSFERMEEEGDVATAFTMAR
Sbjct: 61  VAFLASFSSQGVIFEQLSVIYALPRLFVASFTLVLPFFPTGSFERMEEEGDVATAFTMAR 120

Query: 121 ILSNIPISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIA 180
           +LSNIPIS+GGP SV+IYDIHALQERFYFGD+VLPLFETGIPLLKQRLHQLPD+D +V+A
Sbjct: 121 MLSNIPISKGGPNSVLIYDIHALQERFYFGDHVLPLFETGIPLLKQRLHQLPDSDKIVVA 180

Query: 181 FPDDGAWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIE 240
           FPDDGAWKRFHK LD +P+V+C KVRE DKRIVRIKEGNPAG HVVIVDDLVQSGGTLIE
Sbjct: 181 FPDDGAWKRFHKQLDHFPMVVCNKVREGDKRIVRIKEGNPAGYHVVIVDDLVQSGGTLIE 240

Query: 241 CQKVLAAHGAAKVSAYVTHGVFPKQSWKRFLHKN-GLEMAFSYFWITDSCPHTVKAIADK 300
           CQKVLAAHGAAKVSAYVTHGVFPK+SW+RF HK+ G E AF+YFWITDSCP TVKA+A+K
Sbjct: 241 CQKVLAAHGAAKVSAYVTHGVFPKRSWERFTHKDEGSEKAFAYFWITDSCPLTVKAVANK 300

Query: 301 APFEVLSLAGSIADALVI 318
           APFEVLSLAGSIADAL I
Sbjct: 301 APFEVLSLAGSIADALQI 318

BLAST of Cucsa.185460 vs. TrEMBL
Match: W9R9E0_9ROSA (Ribose-phosphate pyrophosphokinase 4 OS=Morus notabilis GN=L484_014166 PE=4 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 2.0e-152
Identity = 262/315 (83.17%), Postives = 291/315 (92.38%), Query Frame = 1

Query: 1   MAKPPKKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNH 60
           M K  KK ++LFYC ECE+LAQKVA+QSD ITLQTIKWR+FDDGFPNI+INNAQELRG H
Sbjct: 1   MEKSQKKRVYLFYCSECEDLAQKVASQSDFITLQTIKWRSFDDGFPNIFINNAQELRGQH 60

Query: 61  VAFLASFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMAR 120
           VAFLASFS QG IFEQ+SVIY L RLF ASFTLVLPFFPTGSFERMEEEGDVATAFT+AR
Sbjct: 61  VAFLASFSSQGVIFEQLSVIYALTRLFVASFTLVLPFFPTGSFERMEEEGDVATAFTLAR 120

Query: 121 ILSNIPISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIA 180
           +L+NIPISRGGPTS+V+YDIHALQERFYFGD+VLPLFETGIPLLKQRLH+LPD+D +V+A
Sbjct: 121 MLTNIPISRGGPTSLVVYDIHALQERFYFGDHVLPLFETGIPLLKQRLHKLPDSDKIVVA 180

Query: 181 FPDDGAWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIE 240
           FPDDGAWKRFHK LD +P V+C KV E DKRIVRIKEGNPAG HVVIVDDLVQSGGTLIE
Sbjct: 181 FPDDGAWKRFHKQLDHFPTVVCNKVHEGDKRIVRIKEGNPAGYHVVIVDDLVQSGGTLIE 240

Query: 241 CQKVLAAHGAAKVSAYVTHGVFPKQSWKRFLHKNGLEMAFSYFWITDSCPHTVKAIADKA 300
           C+KVL AHGAAKVSAYVTHGVFPK+SW++F HK+GLE AF+YFWITDSCP TVKAIA++A
Sbjct: 241 CRKVLVAHGAAKVSAYVTHGVFPKRSWEQFTHKDGLEKAFTYFWITDSCPLTVKAIANRA 300

Query: 301 PFEVLSLAGSIADAL 316
           PFEVLSLAGSI++AL
Sbjct: 301 PFEVLSLAGSISEAL 315

BLAST of Cucsa.185460 vs. TAIR10
Match: AT2G42910.1 (AT2G42910.1 Phosphoribosyltransferase family protein)

HSP 1 Score: 528.9 bits (1361), Expect = 2.2e-150
Identity = 259/313 (82.75%), Postives = 280/313 (89.46%), Query Frame = 1

Query: 6   KKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNHVAFLA 65
           KK + LFYC ECEELA+ +AA+SD ITLQ+I WR+F DGFPN++INNA ++RG HVAFLA
Sbjct: 25  KKKVHLFYCLECEELARNIAAESDHITLQSINWRSFADGFPNLFINNAHDIRGQHVAFLA 84

Query: 66  SFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMARILSNI 125
           SFS    IFEQISVIY LPRLF ASFTLVLPFFPTGSFERMEEEGDVATAFTMARI+SNI
Sbjct: 85  SFSSPAVIFEQISVIYLLPRLFVASFTLVLPFFPTGSFERMEEEGDVATAFTMARIVSNI 144

Query: 126 PISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIAFPDDG 185
           PISRGGPTSVVIYDIHALQERFYF D VLPLFETGIPLL +RL QLP+ + V++AFPDDG
Sbjct: 145 PISRGGPTSVVIYDIHALQERFYFADQVLPLFETGIPLLTKRLQQLPETEKVIVAFPDDG 204

Query: 186 AWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIECQKVL 245
           AWKRFHKLLD YP V+CTKVRE DKRIVR+KEGNPAG HVVIVDDLVQSGGTLIECQKVL
Sbjct: 205 AWKRFHKLLDHYPTVVCTKVREGDKRIVRLKEGNPAGCHVVIVDDLVQSGGTLIECQKVL 264

Query: 246 AAHGAAKVSAYVTHGVFPKQSWKRFLH-KNGLEMAFSYFWITDSCPHTVKAIADKAPFEV 305
           AAHGA KVSAYVTHGVFPK SW+RF H KNGLE AF+YFWITDSCP TVKAI +KAPFEV
Sbjct: 265 AAHGAVKVSAYVTHGVFPKSSWERFTHKKNGLEEAFAYFWITDSCPQTVKAIGNKAPFEV 324

Query: 306 LSLAGSIADALVI 318
           LSLAGSIADAL I
Sbjct: 325 LSLAGSIADALQI 337

BLAST of Cucsa.185460 vs. TAIR10
Match: AT1G10700.1 (AT1G10700.1 phosphoribosyl pyrophosphate (PRPP) synthase 3)

HSP 1 Score: 456.4 bits (1173), Expect = 1.4e-128
Identity = 221/313 (70.61%), Postives = 257/313 (82.11%), Query Frame = 1

Query: 7   KHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNHVAFLAS 66
           K + LF+ +E  +LA+++ A+SD I L++I W+ FDDGFPN++I NAQ +RG HVAFLAS
Sbjct: 99  KRVCLFHSDETRDLAERIVAKSDCIELRSINWKKFDDGFPNLFIQNAQGIRGQHVAFLAS 158

Query: 67  FSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMARILSNIP 126
           FS    IFEQ+SVIY LP+LF +SFTLVLPFFPTG+ ERME+EGDVATAFT+ARILSNIP
Sbjct: 159 FSSPAVIFEQLSVIYALPKLFVSSFTLVLPFFPTGTSERMEDEGDVATAFTLARILSNIP 218

Query: 127 ISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIAFPDDGA 186
            SRGGPTS+V +DIHALQERFYFGD +LP FE+GIPLLK RL  LPD+D++ IAFPDDGA
Sbjct: 219 TSRGGPTSLVTFDIHALQERFYFGDTILPCFESGIPLLKSRLQSLPDSDNISIAFPDDGA 278

Query: 187 WKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIECQKVLA 246
           WKRFHK L  YP ++C KVR  DKRIVRIKEG+  GRHVVIVDDLVQSGGTLIECQKVLA
Sbjct: 279 WKRFHKQLQHYPTIVCNKVRMGDKRIVRIKEGDAEGRHVVIVDDLVQSGGTLIECQKVLA 338

Query: 247 AHGAAKVSAYVTHGVFPKQSWKRFL--HKNGLEMAFSYFWITDSCPHTVKAIADKAPFEV 306
           AHGAAK+SAYVTHG+FP+ SWKRF    K       SYFWITDSC  TVK + +K PFEV
Sbjct: 339 AHGAAKISAYVTHGIFPRSSWKRFKLDTKGDPAEGLSYFWITDSCGMTVKEVMNKPPFEV 398

Query: 307 LSLAGSIADALVI 318
           LSLAGSIA AL +
Sbjct: 399 LSLAGSIASALQV 411

BLAST of Cucsa.185460 vs. TAIR10
Match: AT2G35390.2 (AT2G35390.2 Phosphoribosyltransferase family protein)

HSP 1 Score: 63.5 bits (153), Expect = 2.6e-10
Identity = 66/257 (25.68%), Postives = 119/257 (46.30%), Query Frame = 1

Query: 20  LAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNHVAFL--ASFSHQGAIFEQI 79
           LAQ++A     + L  +  + F DG   IY+   + +RG  V  +          + E +
Sbjct: 104 LAQEIAWYM-GLDLGKVNIKRFADG--EIYVQLQESVRGCDVYLVQPTCTPTNENLMELL 163

Query: 80  SVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMARILSNIPISRGGPTSVVI 139
            ++    R  A   T V+P+F     +R + +G  + A   A++++N+ I+  G   V+ 
Sbjct: 164 IMVDACRRASAKKVTAVIPYFGYARADR-KTQGRESIA---AKLVANL-ITEAGADRVLA 223

Query: 140 YDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIAFPDDGAWKR---FHKLL 199
            D+H+ Q   YF   V  ++   + L       +P ++D+V+  PD G   R   F K L
Sbjct: 224 CDLHSGQSMGYFDIPVDHVYCQPVILDYLASKSIP-SEDLVVVSPDVGGVARARAFAKKL 283

Query: 200 DRYPVVICTKVREEDKRIVRIKE--GNPAGRHVVIVDDLVQSGGTLIECQKVLAAHGAAK 259
              P+ I  K R     +  +    G+  G+  ++VDD++ + GT+++   +L   GA +
Sbjct: 284 SDAPLAIVDK-RRSGHNVAEVMNLIGDVRGKVAIMVDDMIDTAGTIVKGAALLHQEGARE 343

Query: 260 VSAYVTHGVFPKQSWKR 270
           V A  TH VF   + +R
Sbjct: 344 VYACCTHAVFSPPAIER 350

BLAST of Cucsa.185460 vs. TAIR10
Match: AT1G32380.1 (AT1G32380.1 phosphoribosyl pyrophosphate (PRPP) synthase 2)

HSP 1 Score: 62.0 bits (149), Expect = 7.6e-10
Identity = 65/257 (25.29%), Postives = 119/257 (46.30%), Query Frame = 1

Query: 20  LAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNHVAFL--ASFSHQGAIFEQI 79
           L+Q++A     + L  +  + F DG   IY+   + +RG  V  +          + E +
Sbjct: 101 LSQEIAWYM-GLELGKVSIKRFADG--EIYVQLKESVRGCDVFLVQPTCTPTNENLMELL 160

Query: 80  SVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMARILSNIPISRGGPTSVVI 139
            ++    R  A   T V+P+F     +R + +G  + A   A++++N+ I+  G   V+ 
Sbjct: 161 IMVDACRRASAKKVTAVIPYFGYARADR-KTQGRESIA---AKLVANL-ITEAGADRVLA 220

Query: 140 YDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIAFPDDGAWKR---FHKLL 199
            D+H+ Q   YF   V  ++   + +L     +   ++D+V+  PD G   R   F K L
Sbjct: 221 CDLHSGQSMGYFDIPVDHVYCQPV-ILDYLASKSISSEDLVVVSPDVGGVARARAFAKKL 280

Query: 200 DRYPVVICTKVREEDKRIVRIKE--GNPAGRHVVIVDDLVQSGGTLIECQKVLAAHGAAK 259
              P+ I  K R     +  +    G+  G+  V+VDD++ + GT+++   +L   GA +
Sbjct: 281 SDAPLAIVDK-RRHGHNVAEVMNLIGDVKGKVAVMVDDIIDTAGTIVKGAALLHEEGARE 340

Query: 260 VSAYVTHGVFPKQSWKR 270
           V A  TH VF   + +R
Sbjct: 341 VYACCTHAVFSPPAIER 347

BLAST of Cucsa.185460 vs. NCBI nr
Match: gi|449444763|ref|XP_004140143.1| (PREDICTED: ribose-phosphate pyrophosphokinase 4 [Cucumis sativus])

HSP 1 Score: 642.9 bits (1657), Expect = 3.0e-181
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 1

Query: 1   MAKPPKKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNH 60
           MAKPPKKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNH
Sbjct: 1   MAKPPKKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNH 60

Query: 61  VAFLASFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMAR 120
           VAFLASFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMAR
Sbjct: 61  VAFLASFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMAR 120

Query: 121 ILSNIPISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIA 180
           ILSNIPISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIA
Sbjct: 121 ILSNIPISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIA 180

Query: 181 FPDDGAWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIE 240
           FPDDGAWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIE
Sbjct: 181 FPDDGAWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIE 240

Query: 241 CQKVLAAHGAAKVSAYVTHGVFPKQSWKRFLHKNGLEMAFSYFWITDSCPHTVKAIADKA 300
           CQKVLAAHGAAKVSAYVTHGVFPKQSWKRFLHKNGLEMAFSYFWITDSCPHTVKAIADKA
Sbjct: 241 CQKVLAAHGAAKVSAYVTHGVFPKQSWKRFLHKNGLEMAFSYFWITDSCPHTVKAIADKA 300

Query: 301 PFEVLSLAGSIADALVI 318
           PFEVLSLAGSIADALVI
Sbjct: 301 PFEVLSLAGSIADALVI 317

BLAST of Cucsa.185460 vs. NCBI nr
Match: gi|595938860|ref|XP_007215700.1| (hypothetical protein PRUPE_ppa008810mg [Prunus persica])

HSP 1 Score: 556.2 bits (1432), Expect = 3.7e-155
Identity = 269/317 (84.86%), Postives = 293/317 (92.43%), Query Frame = 1

Query: 1   MAKPPKKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNH 60
           M    KK +FLFYC ECE+LA++VA+QSD ITLQTIKWRTFDDGFPNI+INNAQ+LRG H
Sbjct: 1   MENSQKKQVFLFYCAECEDLARQVASQSDHITLQTIKWRTFDDGFPNIFINNAQDLRGQH 60

Query: 61  VAFLASFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMAR 120
           VAFLASFS QG IFEQ+SVIY LPRLF ASFTLVLPFFPTGSFERMEEEGDVATAFTMAR
Sbjct: 61  VAFLASFSSQGVIFEQLSVIYALPRLFVASFTLVLPFFPTGSFERMEEEGDVATAFTMAR 120

Query: 121 ILSNIPISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIA 180
           +LSNIPIS+GGP SV+IYDIHALQERFYFGD+VLPLFETGIPLLKQRLHQLPD+D +V+A
Sbjct: 121 MLSNIPISKGGPNSVLIYDIHALQERFYFGDHVLPLFETGIPLLKQRLHQLPDSDKIVVA 180

Query: 181 FPDDGAWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIE 240
           FPDDGAWKRFHK LD +P+V+C KVRE DKRIVRIKEGNPAG HVVIVDDLVQSGGTLIE
Sbjct: 181 FPDDGAWKRFHKQLDHFPMVVCNKVREGDKRIVRIKEGNPAGYHVVIVDDLVQSGGTLIE 240

Query: 241 CQKVLAAHGAAKVSAYVTHGVFPKQSWKRFLHKNGLEMAFSYFWITDSCPHTVKAIADKA 300
           CQKVLAAHGAAKVSAYVTHGVFPK+SW+RF HK+G E AF+YFWITDSCP TVKA+A+KA
Sbjct: 241 CQKVLAAHGAAKVSAYVTHGVFPKRSWERFTHKDGSEKAFAYFWITDSCPLTVKAVANKA 300

Query: 301 PFEVLSLAGSIADALVI 318
           PFEVLSLAGSIADAL I
Sbjct: 301 PFEVLSLAGSIADALQI 317

BLAST of Cucsa.185460 vs. NCBI nr
Match: gi|703120695|ref|XP_010102157.1| (Ribose-phosphate pyrophosphokinase 4 [Morus notabilis])

HSP 1 Score: 553.1 bits (1424), Expect = 3.1e-154
Identity = 266/310 (85.81%), Postives = 292/310 (94.19%), Query Frame = 1

Query: 6   KKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNHVAFLA 65
           KK ++LFYC ECE+LAQKVA+QSD ITLQTIKWR+FDDGFPNI+INNAQ LRG HVAFLA
Sbjct: 6   KKQVYLFYCSECEDLAQKVASQSDFITLQTIKWRSFDDGFPNIFINNAQVLRGQHVAFLA 65

Query: 66  SFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMARILSNI 125
           SFS QG IFEQ+SVIY LPRLF ASFTLVLPFFPTGSFERMEEEGDVATAFT+AR+LSNI
Sbjct: 66  SFSSQGVIFEQLSVIYALPRLFVASFTLVLPFFPTGSFERMEEEGDVATAFTLARMLSNI 125

Query: 126 PISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIAFPDDG 185
           PISRGGPTS+VIYDIHALQERFYFGD+VLPLFETGIPLLKQRLHQLPD+D +V+AFPDDG
Sbjct: 126 PISRGGPTSLVIYDIHALQERFYFGDHVLPLFETGIPLLKQRLHQLPDSDKIVVAFPDDG 185

Query: 186 AWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIECQKVL 245
           AWKRFHK LD +P+V+C KVRE DKRIVRIKEGNPAG HVVIVDDLVQSGGTLIECQKVL
Sbjct: 186 AWKRFHKQLDHFPMVVCNKVREGDKRIVRIKEGNPAGYHVVIVDDLVQSGGTLIECQKVL 245

Query: 246 AAHGAAKVSAYVTHGVFPKQSWKRFLHKNGLEMAFSYFWITDSCPHTVKAIADKAPFEVL 305
           AAHGA+KVSAYVTHGVFPK+SW+RF HK+GLE AF+YFWITDSCP TVKAIA++APFEVL
Sbjct: 246 AAHGASKVSAYVTHGVFPKRSWERFTHKDGLEKAFAYFWITDSCPLTVKAIANRAPFEVL 305

Query: 306 SLAGSIADAL 316
           SLAGSI++AL
Sbjct: 306 SLAGSISEAL 315

BLAST of Cucsa.185460 vs. NCBI nr
Match: gi|595938890|ref|XP_007215701.1| (hypothetical protein PRUPE_ppa008810mg [Prunus persica])

HSP 1 Score: 551.6 bits (1420), Expect = 9.0e-154
Identity = 269/318 (84.59%), Postives = 293/318 (92.14%), Query Frame = 1

Query: 1   MAKPPKKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNH 60
           M    KK +FLFYC ECE+LA++VA+QSD ITLQTIKWRTFDDGFPNI+INNAQ+LRG H
Sbjct: 1   MENSQKKQVFLFYCAECEDLARQVASQSDHITLQTIKWRTFDDGFPNIFINNAQDLRGQH 60

Query: 61  VAFLASFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMAR 120
           VAFLASFS QG IFEQ+SVIY LPRLF ASFTLVLPFFPTGSFERMEEEGDVATAFTMAR
Sbjct: 61  VAFLASFSSQGVIFEQLSVIYALPRLFVASFTLVLPFFPTGSFERMEEEGDVATAFTMAR 120

Query: 121 ILSNIPISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIA 180
           +LSNIPIS+GGP SV+IYDIHALQERFYFGD+VLPLFETGIPLLKQRLHQLPD+D +V+A
Sbjct: 121 MLSNIPISKGGPNSVLIYDIHALQERFYFGDHVLPLFETGIPLLKQRLHQLPDSDKIVVA 180

Query: 181 FPDDGAWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIE 240
           FPDDGAWKRFHK LD +P+V+C KVRE DKRIVRIKEGNPAG HVVIVDDLVQSGGTLIE
Sbjct: 181 FPDDGAWKRFHKQLDHFPMVVCNKVREGDKRIVRIKEGNPAGYHVVIVDDLVQSGGTLIE 240

Query: 241 CQKVLAAHGAAKVSAYVTHGVFPKQSWKRFLHKN-GLEMAFSYFWITDSCPHTVKAIADK 300
           CQKVLAAHGAAKVSAYVTHGVFPK+SW+RF HK+ G E AF+YFWITDSCP TVKA+A+K
Sbjct: 241 CQKVLAAHGAAKVSAYVTHGVFPKRSWERFTHKDEGSEKAFAYFWITDSCPLTVKAVANK 300

Query: 301 APFEVLSLAGSIADALVI 318
           APFEVLSLAGSIADAL I
Sbjct: 301 APFEVLSLAGSIADALQI 318

BLAST of Cucsa.185460 vs. NCBI nr
Match: gi|470136165|ref|XP_004303868.1| (PREDICTED: ribose-phosphate pyrophosphokinase 4 [Fragaria vesca subsp. vesca])

HSP 1 Score: 547.0 bits (1408), Expect = 2.2e-152
Identity = 264/316 (83.54%), Postives = 292/316 (92.41%), Query Frame = 1

Query: 1   MAKPPKKHIFLFYCEECEELAQKVAAQSDSITLQTIKWRTFDDGFPNIYINNAQELRGNH 60
           M K PKK ++LFYC ECE+LA++VA+QSD ITLQTIKWR FDDGFPNI+INNAQ+LRG H
Sbjct: 3   MDKSPKKQVYLFYCAECEDLARQVASQSDLITLQTIKWRNFDDGFPNIFINNAQDLRGQH 62

Query: 61  VAFLASFSHQGAIFEQISVIYNLPRLFAASFTLVLPFFPTGSFERMEEEGDVATAFTMAR 120
           VAFLASFS QG IFEQ+SVIY LP+LF ASF+LVLPFFPTGSFERMEEEGDVATAFTMAR
Sbjct: 63  VAFLASFSSQGVIFEQLSVIYALPKLFVASFSLVLPFFPTGSFERMEEEGDVATAFTMAR 122

Query: 121 ILSNIPISRGGPTSVVIYDIHALQERFYFGDNVLPLFETGIPLLKQRLHQLPDADDVVIA 180
           +LSNIPIS+GGPTS+VIYDIHALQERFYFGD+VLPLFETGIPLLK+RLHQLPD+D +V+A
Sbjct: 123 MLSNIPISKGGPTSLVIYDIHALQERFYFGDHVLPLFETGIPLLKERLHQLPDSDKIVVA 182

Query: 181 FPDDGAWKRFHKLLDRYPVVICTKVREEDKRIVRIKEGNPAGRHVVIVDDLVQSGGTLIE 240
           FPDDGAWKRFHK LD +P+V+C KVRE DKRIVRIKEGNPAG HVVIVDDLVQSGGTLIE
Sbjct: 183 FPDDGAWKRFHKQLDHFPMVVCNKVREGDKRIVRIKEGNPAGFHVVIVDDLVQSGGTLIE 242

Query: 241 CQKVLAAHGAAKVSAYVTHGVFPKQSWKRFLHKNG-LEMAFSYFWITDSCPHTVKAIADK 300
           CQKVLAAHGAAKVSAYVTHGVFPK+SW+RF HKNG LE  F+YFWITDSCP TV A+ +K
Sbjct: 243 CQKVLAAHGAAKVSAYVTHGVFPKRSWERFTHKNGELENPFAYFWITDSCPLTVTAVKNK 302

Query: 301 APFEVLSLAGSIADAL 316
           APFE+LSLAGSIADAL
Sbjct: 303 APFEILSLAGSIADAL 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KPRS4_ARATH3.9e-14982.75Ribose-phosphate pyrophosphokinase 4 OS=Arabidopsis thaliana GN=PRS4 PE=1 SV=2[more]
KPRS4_SPIOL5.1e-14981.76Ribose-phosphate pyrophosphokinase 4 OS=Spinacia oleracea GN=PRS4 PE=2 SV=1[more]
KPRS4_ORYSJ1.3e-14180.25Ribose-phosphate pyrophosphokinase 4 OS=Oryza sativa subsp. japonica GN=Os02g071... [more]
KPRS3_SPIOL2.8e-13171.88Ribose-phosphate pyrophosphokinase 3, mitochondrial OS=Spinacia oleracea GN=PRS3... [more]
KPRS3_ORYSJ2.6e-12970.61Ribose-phosphate pyrophosphokinase 3, chloroplastic OS=Oryza sativa subsp. japon... [more]
Match NameE-valueIdentityDescription
A0A0A0KI24_CUCSA2.1e-181100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_6G423440 PE=3 SV=1[more]
M5WT69_PRUPE2.6e-15584.86Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008810mg PE=3 SV=1[more]
W9RT02_9ROSA2.2e-15485.81Ribose-phosphate pyrophosphokinase 4 OS=Morus notabilis GN=L484_021391 PE=4 SV=1[more]
M5XCT7_PRUPE6.3e-15484.59Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008810mg PE=3 SV=1[more]
W9R9E0_9ROSA2.0e-15283.17Ribose-phosphate pyrophosphokinase 4 OS=Morus notabilis GN=L484_014166 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G42910.12.2e-15082.75 Phosphoribosyltransferase family protein[more]
AT1G10700.11.4e-12870.61 phosphoribosyl pyrophosphate (PRPP) synthase 3[more]
AT2G35390.22.6e-1025.68 Phosphoribosyltransferase family protein[more]
AT1G32380.17.6e-1025.29 phosphoribosyl pyrophosphate (PRPP) synthase 2[more]
Match NameE-valueIdentityDescription
gi|449444763|ref|XP_004140143.1|3.0e-181100.00PREDICTED: ribose-phosphate pyrophosphokinase 4 [Cucumis sativus][more]
gi|595938860|ref|XP_007215700.1|3.7e-15584.86hypothetical protein PRUPE_ppa008810mg [Prunus persica][more]
gi|703120695|ref|XP_010102157.1|3.1e-15485.81Ribose-phosphate pyrophosphokinase 4 [Morus notabilis][more]
gi|595938890|ref|XP_007215701.1|9.0e-15484.59hypothetical protein PRUPE_ppa008810mg [Prunus persica][more]
gi|470136165|ref|XP_004303868.1|2.2e-15283.54PREDICTED: ribose-phosphate pyrophosphokinase 4 [Fragaria vesca subsp. vesca][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000836PRibTrfase_dom
IPR005946Rib-P_diPkinase
Vocabulary: Biological Process
TermDefinition
GO:0009116nucleoside metabolic process
GO:0009165nucleotide biosynthetic process
Vocabulary: Molecular Function
TermDefinition
GO:0000287magnesium ion binding
GO:0004749ribose phosphate diphosphokinase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006783 heme biosynthetic process
biological_process GO:0009116 nucleoside metabolic process
biological_process GO:0006098 pentose-phosphate shunt
biological_process GO:0016310 phosphorylation
biological_process GO:0006144 purine nucleobase metabolic process
biological_process GO:0009220 pyrimidine ribonucleotide biosynthetic process
biological_process GO:0009165 nucleotide biosynthetic process
cellular_component GO:0005829 cytosol
cellular_component GO:0005886 plasma membrane
cellular_component GO:0009506 plasmodesma
cellular_component GO:0005575 cellular_component
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004749 ribose phosphate diphosphokinase activity
molecular_function GO:0016757 transferase activity, transferring glycosyl groups

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.185460.1Cucsa.185460.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000836Phosphoribosyltransferase domainPFAMPF00156Pribosyltrancoord: 172..262
score: 2.5
IPR005946Ribose-phosphate diphosphokinaseTIGRFAMsTIGR01251TIGR01251coord: 11..315
score: 5.2
NoneNo IPR availablePANTHERPTHR10210RIBOSE-PHOSPHATE PYROPHOSPHOKINASEcoord: 1..315
score: 2.7E
NoneNo IPR availablePANTHERPTHR10210:SF34RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 4coord: 1..315
score: 2.7E
NoneNo IPR availableSMARTSM01400Pribosyltran_N_2coord: 9..126
score: 1.0

The following gene(s) are paralogous to this gene:

None