Cucsa.169890 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.169890
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionElongation factor 4
Locationscaffold01174 : 113190 .. 113831 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGTAGAGCTTTCTTCCGCGCCTCTCTTATCCACATGCACTCAACATCTTCGTCCTTTCCAATCAAGAACAAACATCCATTTCCATTTCCCTTCACTTCCAATTCCTCTATCCCTCACCACTTTCCCTTTCTCTTCTTCAACTGCCACAACCAGAGGCAAGGTCCTCTGTCAGACTGCTGGAGCGCATTTCTTCGATGCTCAAAACGCTGTTGAAGCAGGTCAAAATCGACTGCTGAAGGTTCTCTCCCTCACTATGTTCACATTTTTTTTTTATCCGTCTTTTATTCTCTTTTTGCTCGATTGAATTTGCTTCTTCTATACTCATCCGAGCATTCGGTAACTATGTCACAAAAATGCAGGTTCCTATATCGCACATCAGAAACTTCAGCATTATTGCGCACATTGATCATGGTAAATCAACTTTGGCCGATAAATTGCTTCAGATGACTGGTACCGTTCAGACTCGAGAAATGAAGGATCAGTTTCTTGATAACATGGATTTGGAGAGAGAAAGAGGCATCACAATTAAACTACAGGTCAGTTTACTCATTTGGTTTCACTTTGATGCAATTAATCGTTTATTCAGATTCCCGTAAGAATTGGAACAATTAAGTATTTACACCGAGCTATATTATAA

mRNA sequence

ATGGCGGTAGAGCTTTCTTCCGCGCCTCTCTTATCCACATGCACTCAACATCTTCGTCCTTTCCAATCAAGAACAAACATCCATTTCCATTTCCCTTCACTTCCAATTCCTCTATCCCTCACCACTTTCCCTTTCTCTTCTTCAACTGCCACAACCAGAGGCAAGGTCCTCTGTCAGACTGCTGGAGCGCATTTCTTCGATGCTCAAAACGCTGTTGAAGCAGGTCAAAATCGACTGCTGAAGGTTCCTATATCGCACATCAGAAACTTCAGCATTATTGCGCACATTGATCATGGTAAATCAACTTTGGCCGATAAATTGCTTCAGATGACTGGTACCGTTCAGACTCGAGAAATGAAGGATCAGTTTCTTGATAACATGGATTTGGAGAGAGAAAGAGGCATCACAATTAAACTACAGATTCCCGTAAGAATTGGAACAATTAAGTATTTACACCGAGCTATATTATAA

Coding sequence (CDS)

ATGGCGGTAGAGCTTTCTTCCGCGCCTCTCTTATCCACATGCACTCAACATCTTCGTCCTTTCCAATCAAGAACAAACATCCATTTCCATTTCCCTTCACTTCCAATTCCTCTATCCCTCACCACTTTCCCTTTCTCTTCTTCAACTGCCACAACCAGAGGCAAGGTCCTCTGTCAGACTGCTGGAGCGCATTTCTTCGATGCTCAAAACGCTGTTGAAGCAGGTCAAAATCGACTGCTGAAGGTTCCTATATCGCACATCAGAAACTTCAGCATTATTGCGCACATTGATCATGGTAAATCAACTTTGGCCGATAAATTGCTTCAGATGACTGGTACCGTTCAGACTCGAGAAATGAAGGATCAGTTTCTTGATAACATGGATTTGGAGAGAGAAAGAGGCATCACAATTAAACTACAGATTCCCGTAAGAATTGGAACAATTAAGTATTTACACCGAGCTATATTATAA

Protein sequence

MAVELSSAPLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSLTTFPFSSSTATTRGKVLCQTAGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMKDQFLDNMDLERERGITIKLQIPVRIGTIKYLHRAIL*
Homology
[Close]
BLAST of Cucsa.169890 vs. Swiss-Prot
Match: GUFP_RICCO (Translation factor GUF1 homolog, chloroplastic OS=Ricinus communis GN=RCOM_1767360 PE=3 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 1.8e-30
Identity = 84/149 (56.38%), Postives = 99/149 (66.44%), Query Frame = 1 

Query: 1   MAVELSSAP-LLSTCTQHLRPFQSRTNIHFHFPSLPIPLSLTTFPFSSSTAT-TRGKVLC 60
           MA +LSS+  LL +      PF   TN         + LS T   ++S  A  +R ++LC
Sbjct: 1   MATDLSSSSTLLLSRNCKTPPFYHTTN--------SLSLSKTHHLYASRNAVVSRLRLLC 60

Query: 61  QTAGAH-------FFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMT 120
           QT G+          D Q A + GQ+RL KVPIS+IRNF IIAHIDHGKSTLADKLLQ+T
Sbjct: 61  QTTGSTQSTPTSGVSDLQFAAQVGQHRLSKVPISNIRNFCIIAHIDHGKSTLADKLLQVT 120

Query: 121 GTVQTREMKDQFLDNMDLERERGITIKLQ 141
           GTVQ REMK+QFLDNMDLERERGITIKLQ
Sbjct: 121 GTVQQREMKEQFLDNMDLERERGITIKLQ 141

[Close]
BLAST of Cucsa.169890 vs. Swiss-Prot
Match: GUFP_VITVI (Translation factor GUF1 homolog, chloroplastic OS=Vitis vinifera GN=VITISV_013255 PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 2.0e-29
Identity = 79/125 (63.20%), Postives = 85/125 (68.00%), Query Frame = 1 

Query: 28  HFHFPSLPIPLSLTTFPFSSSTATT--------RGKVLCQ-TAGAHF---FDAQNAVEAG 87
           H H  S P   S    PFS   +TT        R  V+ Q TAG       D   A  +G
Sbjct: 18  HHHHXSTPFYFS----PFSPHLSTTLTSRRRSLRSAVVAQSTAGTQSKAPSDVDLAAVSG 77

Query: 88  QNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMKDQFLDNMDLERERGI 141
           Q+RLLKVPIS+IRNF IIAHIDHGKSTLADKLLQMTGTVQ REMK+QFLDNMDLERERGI
Sbjct: 78  QDRLLKVPISNIRNFCIIAHIDHGKSTLADKLLQMTGTVQKREMKEQFLDNMDLERERGI 137

[Close]
BLAST of Cucsa.169890 vs. Swiss-Prot
Match: GUFP_ORYSJ (Translation factor GUF1 homolog, chloroplastic OS=Oryza sativa subsp. japonica GN=Os02g0157700 PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 2.0e-29
Identity = 72/109 (66.06%), Postives = 84/109 (77.06%), Query Frame = 1 

Query: 32  PSLPIPLSLTTFPFSSSTATTRGKVLCQTAGAHFFDAQNAVEAGQNRLLKVPISHIRNFS 91
           P+ P+P S    P + S A  RG+V+C  A A      +  +AGQ+RL KVP+S+IRNFS
Sbjct: 26  PAAPLP-SAPPRPRALSAAP-RGRVVCPAAPAS--SPASTTDAGQDRLQKVPVSNIRNFS 85

Query: 92  IIAHIDHGKSTLADKLLQMTGTVQTREMKDQFLDNMDLERERGITIKLQ 141
           IIAHIDHGKSTLADKLL++TGTVQ REMK QFLDNMDLERERGITIKLQ
Sbjct: 86  IIAHIDHGKSTLADKLLELTGTVQKREMKQQFLDNMDLERERGITIKLQ 130

[Close]
BLAST of Cucsa.169890 vs. Swiss-Prot
Match: GUFP_POPTR (Translation factor GUF1 homolog, chloroplastic OS=Populus trichocarpa GN=POPTRDRAFT_815670 PE=3 SV=2)

HSP 1 Score: 125.9 bits (315), Expect = 3.8e-28
Identity = 67/89 (75.28%), Postives = 71/89 (79.78%), Query Frame = 1 

Query: 52  TRGKVLCQTAGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMT 111
           TR  + CQT       A  A  AGQ+RL KVPI +IRNF IIAHIDHGKSTLADKLLQMT
Sbjct: 48  TRLHLRCQTTTGTPSAADFAAAAGQDRLRKVPIKNIRNFCIIAHIDHGKSTLADKLLQMT 107

Query: 112 GTVQTREMKDQFLDNMDLERERGITIKLQ 141
           GTVQ REMK+QFLDNMDLERERGITIKLQ
Sbjct: 108 GTVQKREMKEQFLDNMDLERERGITIKLQ 136

[Close]
BLAST of Cucsa.169890 vs. Swiss-Prot
Match: GUFP_ARATH (Translation factor GUF1 homolog, chloroplastic OS=Arabidopsis thaliana GN=At5g08650 PE=2 SV=2)

HSP 1 Score: 125.6 bits (314), Expect = 4.9e-28
Identity = 65/93 (69.89%), Postives = 76/93 (81.72%), Query Frame = 1 

Query: 55  KVLCQT-------AGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKL 114
           ++LCQ        +G     ++ A  +GQ+RLLKVPIS+IRNFSIIAHIDHGKSTLADKL
Sbjct: 47  QILCQATAGTEPQSGLSVSGSKLAARSGQDRLLKVPISNIRNFSIIAHIDHGKSTLADKL 106

Query: 115 LQMTGTVQTREMKDQFLDNMDLERERGITIKLQ 141
           LQ+TGTVQ R+MK+QFLDNMDLERERGITIKLQ
Sbjct: 107 LQVTGTVQNRDMKEQFLDNMDLERERGITIKLQ 139

[Close]
BLAST of Cucsa.169890 vs. TrEMBL
Match: A0A0A0L295_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G437520 PE=4 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.0e-72
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 1 

Query: 1   MAVELSSAPLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSLTTFPFSSSTATTRGKVLCQT 60
           MAVELSSAPLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSLTTFPFSSSTATTRGKVLCQT
Sbjct: 1   MAVELSSAPLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSLTTFPFSSSTATTRGKVLCQT 60

Query: 61  AGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK 120
           AGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK
Sbjct: 61  AGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK 120

Query: 121 DQFLDNMDLERERGITIKLQ 141
           DQFLDNMDLERERGITIKLQ
Sbjct: 121 DQFLDNMDLERERGITIKLQ 140

[Close]
BLAST of Cucsa.169890 vs. TrEMBL
Match: A0A067K5K4_JATCU (Translation factor GUF1 homolog, chloroplastic OS=Jatropha curcas GN=JCGZ_15356 PE=3 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 2.8e-30
Identity = 85/145 (58.62%), Postives = 95/145 (65.52%), Query Frame = 1 

Query: 1   MAVELSSAPL-LSTCTQHLRPFQSRTNIHFHFPSLPIPLSLTTFPFS-SSTATTRGKVLC 60
           MA +LS   L LS   +   P           PSLP     TT+P S S  A  + ++LC
Sbjct: 1   MATDLSLPTLVLSKIHRKTLPIYLTRKPRLSSPSLP-----TTYPLSISKNAVPKLRLLC 60

Query: 61  QTAGAHFFDAQN---AVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQ 120
           QTAG           A + GQ+RL KVPIS+IRNF IIAHIDHGKSTLADKLLQMTGTVQ
Sbjct: 61  QTAGTQSTGVSGFEFAAQVGQHRLCKVPISNIRNFCIIAHIDHGKSTLADKLLQMTGTVQ 120

Query: 121 TREMKDQFLDNMDLERERGITIKLQ 141
            REMK+QFLDNMDLERERGITIKLQ
Sbjct: 121 QREMKEQFLDNMDLERERGITIKLQ 140

[Close]
BLAST of Cucsa.169890 vs. TrEMBL
Match: A0A0J8DX36_BETVU (Translation factor GUF1 homolog, chloroplastic OS=Beta vulgaris subsp. vulgaris GN=BVRB_008250 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.1e-29
Identity = 79/135 (58.52%), Postives = 93/135 (68.89%), Query Frame = 1 

Query: 21  FQSRTNIHFHFPSLPIPLSLTTFPFSSSTATTRGKVLCQTAGAHFFDA---QNAVEAGQN 80
           F S + ++ HFP           P SS     R KVL QTAGA   D    + A + GQ+
Sbjct: 31  FPSSSGLNGHFPP----------PISSLRTNRRLKVLAQTAGAPSKDVSALELAAKVGQD 90

Query: 81  RLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMKDQFLDNMDLERERGITI 140
           RLLKVP+S+IRNF IIAHIDHGKSTLADKLL++TGTVQ REMK+QFLDNMDLERERGITI
Sbjct: 91  RLLKVPVSNIRNFCIIAHIDHGKSTLADKLLEITGTVQKREMKEQFLDNMDLERERGITI 150

Query: 141 KLQIPVRIGTIKYLH 153
           KLQ       ++Y+H
Sbjct: 151 KLQ----AARMRYVH 151

[Close]
BLAST of Cucsa.169890 vs. TrEMBL
Match: K3YQL7_SETIT (Translation factor GUF1 homolog, chloroplastic OS=Setaria italica GN=SETIT_016561mg PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 9.0e-29
Identity = 70/94 (74.47%), Postives = 80/94 (85.11%), Query Frame = 1 

Query: 47  SSTATTRGKVLCQTAGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADK 106
           S+ A  RG+VLC  A A    A + ++AGQ+RLLKVPIS+IRNF IIAHIDHGKSTLADK
Sbjct: 41  SAAAAPRGRVLCLAAPAPA--AASTIDAGQDRLLKVPISNIRNFCIIAHIDHGKSTLADK 100

Query: 107 LLQMTGTVQTREMKDQFLDNMDLERERGITIKLQ 141
           LL++TGTVQ REMK+QFLDNMDLERERGITIKLQ
Sbjct: 101 LLELTGTVQKREMKEQFLDNMDLERERGITIKLQ 132

[Close]
BLAST of Cucsa.169890 vs. TrEMBL
Match: A0A0B0PR65_GOSAR (Translation factor GUF1 homolog, mitochondrial OS=Gossypium arboreum GN=F383_15606 PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.5e-28
Identity = 83/152 (54.61%), Postives = 100/152 (65.79%), Query Frame = 1 

Query: 1   MAVELSSA--PLLSTCT---QHLRPFQSRTNIHFHFPSLPIPLSLTTFPFSSSTATTRGK 60
           MA EL S    LLS  T   Q   P  +R+ +       P+P+S T       T  +R +
Sbjct: 1   MAKELCSGVTALLSINTWQGQQHHPHWARSQLS------PLPISAT----HRLTKHSRFR 60

Query: 61  VLCQT----AGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMT 120
           V+CQ+    A    +D +   + GQ+RLLKVP+S+IRNF IIAHIDHGKSTLADKLLQMT
Sbjct: 61  VVCQSVETQAKTEIYDPEFGAKVGQDRLLKVPVSNIRNFCIIAHIDHGKSTLADKLLQMT 120

Query: 121 GTVQTREMKDQFLDNMDLERERGITIKLQIPV 144
           GTVQ REMK+QFLDNMDLERERGITIKLQ  +
Sbjct: 121 GTVQKREMKEQFLDNMDLERERGITIKLQASI 142

[Close]
BLAST of Cucsa.169890 vs. TAIR10
Match: AT5G08650.1 (AT5G08650.1 Small GTP-binding protein)

HSP 1 Score: 125.6 bits (314), Expect = 2.8e-29
Identity = 65/93 (69.89%), Postives = 76/93 (81.72%), Query Frame = 1 

Query: 55  KVLCQT-------AGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKL 114
           ++LCQ        +G     ++ A  +GQ+RLLKVPIS+IRNFSIIAHIDHGKSTLADKL
Sbjct: 47  QILCQATAGTEPQSGLSVSGSKLAARSGQDRLLKVPISNIRNFSIIAHIDHGKSTLADKL 106

Query: 115 LQMTGTVQTREMKDQFLDNMDLERERGITIKLQ 141
           LQ+TGTVQ R+MK+QFLDNMDLERERGITIKLQ
Sbjct: 107 LQVTGTVQNRDMKEQFLDNMDLERERGITIKLQ 139

[Close]
BLAST of Cucsa.169890 vs. TAIR10
Match: AT5G39900.1 (AT5G39900.1 Small GTP-binding protein)

HSP 1 Score: 82.4 bits (202), Expect = 2.7e-16
Identity = 38/62 (61.29%), Postives = 49/62 (79.03%), Query Frame = 1 

Query: 79  LLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMKDQFLDNMDLERERGITIK 138
           L K P   IRNFSIIAHIDHGKSTLAD+L+++TGT++    + Q+LD + +ERERGIT+K
Sbjct: 58  LTKFPSEKIRNFSIIAHIDHGKSTLADRLMELTGTIKKGHGQPQYLDKLQVERERGITVK 117

Query: 139 LQ 141
            Q
Sbjct: 118 AQ 119

[Close]
BLAST of Cucsa.169890 vs. TAIR10
Match: AT5G13650.2 (AT5G13650.2 elongation factor family protein)

HSP 1 Score: 57.8 bits (138), Expect = 7.1e-09
Identity = 25/53 (47.17%), Postives = 40/53 (75.47%), Query Frame = 1 

Query: 86  HIRNFSIIAHIDHGKSTLADKLLQMTGTVQTRE-MKDQFLDNMDLERERGITI 138
           ++RN +I+AH+DHGK+TL D +L+     +  + M+++ +D+ DLERERGITI
Sbjct: 82  NVRNIAIVAHVDHGKTTLVDSMLRQAKVFRDNQVMQERIMDSNDLERERGITI 134

[Close]
BLAST of Cucsa.169890 vs. TAIR10
Match: AT2G31060.3 (AT2G31060.3 elongation factor family protein)

HSP 1 Score: 55.1 bits (131), Expect = 4.6e-08
Identity = 26/57 (45.61%), Postives = 41/57 (71.93%), Query Frame = 1 

Query: 85  SHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMKDQFLDNMDLERERGITIKLQI 142
           + +RN ++IAH+DHGK+TL D+LL+  G     E   + +D+++LERERGITI  ++
Sbjct: 57  NRLRNVAVIAHVDHGKTTLMDRLLRQCGADIPHE---RAMDSINLERERGITISSKV 110

[Close]
BLAST of Cucsa.169890 vs. TAIR10
Match: AT2G45030.1 (AT2G45030.1 Translation elongation factor EFG/EF2 protein)

HSP 1 Score: 54.3 bits (129), Expect = 7.9e-08
Identity = 41/120 (34.17%), Postives = 58/120 (48.33%), Query Frame = 1 

Query: 31  FPSLPIPLSLTTFPFSSSTATTRGKVLCQTAGAHFF------DAQNAVEAGQNRLLKVPI 90
           FP+ P P  L    FSS+   +       T   H         A  AV+  +    K  +
Sbjct: 4   FPTSPAPNLLLRL-FSSNKRASSPTAALLTGDFHLIRHFSAGTAARAVKDEKEPWWKESM 63

Query: 91  SHIRNFSIIAHIDHGKSTLADKLLQMTG------TVQTREMKDQFLDNMDLERERGITIK 139
             +RN  I AHID GK+TL +++L  TG       V+ R+     +D+MDLERE+GITI+
Sbjct: 64  DKLRNIGISAHIDSGKTTLTERVLFYTGRIHEIHEVRGRDGVGAKMDSMDLEREKGITIQ 122

[Close]
BLAST of Cucsa.169890 vs. NCBI nr
Match: gi|778694762|ref|XP_011653860.1| (PREDICTED: translation factor GUF1 homolog, chloroplastic [Cucumis sativus])

HSP 1 Score: 280.8 bits (717), Expect = 1.5e-72
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 1 

Query: 1   MAVELSSAPLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSLTTFPFSSSTATTRGKVLCQT 60
           MAVELSSAPLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSLTTFPFSSSTATTRGKVLCQT
Sbjct: 1   MAVELSSAPLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSLTTFPFSSSTATTRGKVLCQT 60

Query: 61  AGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK 120
           AGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK
Sbjct: 61  AGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK 120

Query: 121 DQFLDNMDLERERGITIKLQ 141
           DQFLDNMDLERERGITIKLQ
Sbjct: 121 DQFLDNMDLERERGITIKLQ 140

[Close]
BLAST of Cucsa.169890 vs. NCBI nr
Match: gi|700199574|gb|KGN54732.1| (hypothetical protein Csa_4G437520 [Cucumis sativus])

HSP 1 Score: 280.8 bits (717), Expect = 1.5e-72
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 1 

Query: 1   MAVELSSAPLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSLTTFPFSSSTATTRGKVLCQT 60
           MAVELSSAPLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSLTTFPFSSSTATTRGKVLCQT
Sbjct: 1   MAVELSSAPLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSLTTFPFSSSTATTRGKVLCQT 60

Query: 61  AGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK 120
           AGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK
Sbjct: 61  AGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK 120

Query: 121 DQFLDNMDLERERGITIKLQ 141
           DQFLDNMDLERERGITIKLQ
Sbjct: 121 DQFLDNMDLERERGITIKLQ 140

[Close]
BLAST of Cucsa.169890 vs. NCBI nr
Match: gi|659069363|ref|XP_008449446.1| (PREDICTED: translation factor GUF1 homolog, chloroplastic isoform X3 [Cucumis melo])

HSP 1 Score: 272.3 bits (695), Expect = 5.2e-70
Identity = 135/140 (96.43%), Postives = 138/140 (98.57%), Query Frame = 1 

Query: 1   MAVELSSAPLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSLTTFPFSSSTATTRGKVLCQT 60
           MA +LS APLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSL+TFPFSSSTATTRGKVLCQT
Sbjct: 1   MAAQLSFAPLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSLSTFPFSSSTATTRGKVLCQT 60

Query: 61  AGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK 120
           AGAHFFDAQNAVEAGQ+RLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK
Sbjct: 61  AGAHFFDAQNAVEAGQDRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK 120

Query: 121 DQFLDNMDLERERGITIKLQ 141
           DQFLDNMDLERERGITIKLQ
Sbjct: 121 DQFLDNMDLERERGITIKLQ 140

[Close]
BLAST of Cucsa.169890 vs. NCBI nr
Match: gi|659069361|ref|XP_008449438.1| (PREDICTED: translation factor GUF1 homolog, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 272.3 bits (695), Expect = 5.2e-70
Identity = 135/140 (96.43%), Postives = 138/140 (98.57%), Query Frame = 1 

Query: 1   MAVELSSAPLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSLTTFPFSSSTATTRGKVLCQT 60
           MA +LS APLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSL+TFPFSSSTATTRGKVLCQT
Sbjct: 1   MAAQLSFAPLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSLSTFPFSSSTATTRGKVLCQT 60

Query: 61  AGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK 120
           AGAHFFDAQNAVEAGQ+RLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK
Sbjct: 61  AGAHFFDAQNAVEAGQDRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK 120

Query: 121 DQFLDNMDLERERGITIKLQ 141
           DQFLDNMDLERERGITIKLQ
Sbjct: 121 DQFLDNMDLERERGITIKLQ 140

[Close]
BLAST of Cucsa.169890 vs. NCBI nr
Match: gi|659069359|ref|XP_008449429.1| (PREDICTED: translation factor GUF1 homolog, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 272.3 bits (695), Expect = 5.2e-70
Identity = 135/140 (96.43%), Postives = 138/140 (98.57%), Query Frame = 1 

Query: 1   MAVELSSAPLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSLTTFPFSSSTATTRGKVLCQT 60
           MA +LS APLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSL+TFPFSSSTATTRGKVLCQT
Sbjct: 1   MAAQLSFAPLLSTCTQHLRPFQSRTNIHFHFPSLPIPLSLSTFPFSSSTATTRGKVLCQT 60

Query: 61  AGAHFFDAQNAVEAGQNRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK 120
           AGAHFFDAQNAVEAGQ+RLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK
Sbjct: 61  AGAHFFDAQNAVEAGQDRLLKVPISHIRNFSIIAHIDHGKSTLADKLLQMTGTVQTREMK 120

Query: 121 DQFLDNMDLERERGITIKLQ 141
           DQFLDNMDLERERGITIKLQ
Sbjct: 121 DQFLDNMDLERERGITIKLQ 140

The following BLAST results are available for this feature:
BLAST of Cucsa.169890 vs. Swiss-Prot
Match NameE-valueIdentityDescription
GUFP_RICCO1.8e-3056.38Translation factor GUF1 homolog, chloroplastic OS=Ricinus communis GN=RCOM_17673... [more]
GUFP_VITVI2.0e-2963.20Translation factor GUF1 homolog, chloroplastic OS=Vitis vinifera GN=VITISV_01325... [more]
GUFP_ORYSJ2.0e-2966.06Translation factor GUF1 homolog, chloroplastic OS=Oryza sativa subsp. japonica G... [more]
GUFP_POPTR3.8e-2875.28Translation factor GUF1 homolog, chloroplastic OS=Populus trichocarpa GN=POPTRDR... [more]
GUFP_ARATH4.9e-2869.89Translation factor GUF1 homolog, chloroplastic OS=Arabidopsis thaliana GN=At5g08... [more]
BLAST of Cucsa.169890 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L295_CUCSA1.0e-72100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G437520 PE=4 SV=1[more]
A0A067K5K4_JATCU2.8e-3058.62Translation factor GUF1 homolog, chloroplastic OS=Jatropha curcas GN=JCGZ_15356 ... [more]
A0A0J8DX36_BETVU1.1e-2958.52Translation factor GUF1 homolog, chloroplastic OS=Beta vulgaris subsp. vulgaris ... [more]
K3YQL7_SETIT9.0e-2974.47Translation factor GUF1 homolog, chloroplastic OS=Setaria italica GN=SETIT_01656... [more]
A0A0B0PR65_GOSAR1.5e-2854.61Translation factor GUF1 homolog, mitochondrial OS=Gossypium arboreum GN=F383_156... [more]
BLAST of Cucsa.169890 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G08650.12.8e-2969.89 Small GTP-binding protein[more]
AT5G39900.12.7e-1661.29 Small GTP-binding protein[more]
AT5G13650.27.1e-0947.17 elongation factor family protein[more]
AT2G31060.34.6e-0845.61 elongation factor family protein[more]
AT2G45030.17.9e-0834.17 Translation elongation factor EFG/EF2 protein[more]
BLAST of Cucsa.169890 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778694762|ref|XP_011653860.1|1.5e-72100.00PREDICTED: translation factor GUF1 homolog, chloroplastic [Cucumis sativus][more]
gi|700199574|gb|KGN54732.1|1.5e-72100.00hypothetical protein Csa_4G437520 [Cucumis sativus][more]
gi|659069363|ref|XP_008449446.1|5.2e-7096.43PREDICTED: translation factor GUF1 homolog, chloroplastic isoform X3 [Cucumis me... [more]
gi|659069361|ref|XP_008449438.1|5.2e-7096.43PREDICTED: translation factor GUF1 homolog, chloroplastic isoform X2 [Cucumis me... [more]
gi|659069359|ref|XP_008449429.1|5.2e-7096.43PREDICTED: translation factor GUF1 homolog, chloroplastic isoform X1 [Cucumis me... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000795TF_GTP-bd_dom
IPR027417P-loop_NTPase
Vocabulary: Molecular Function
TermDefinition
GO:0003924GTPase activity
GO:0005525GTP binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045727 positive regulation of translation
cellular_component GO:0009507 chloroplast
cellular_component GO:0005743 mitochondrial inner membrane
cellular_component GO:0005759 mitochondrial matrix
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0043022 ribosome binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.169890.1Cucsa.169890.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000795Transcription factor, GTP-binding domainPRINTSPR00315ELONGATNFCTcoord: 89..102
score: 2.7E-5coord: 132..140
score: 2.
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3DG3DSA:3.40.50.300coord: 84..140
score: 5.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseunknownSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 81..139
score: 6.09
NoneNo IPR availablePANTHERPTHR23115TRANSLATION FACTORcoord: 72..140
score: 5.4
NoneNo IPR availablePANTHERPTHR23115:SF189TRANSLATION FACTOR GUF1 HOMOLOG, CHLOROPLASTICcoord: 72..140
score: 5.4
NoneNo IPR availablePFAMPF00009GTP_EFTUcoord: 86..140
score: 4.9

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.169890Csa4G437520Cucumber (Chinese Long) v2cgycuB259
Cucsa.169890MELO3C003261Melon (DHL92) v3.5.1cgymeB303
Cucsa.169890CSPI04G19420Wild cucumber (PI 183967)cgycpiB267
Cucsa.169890Lsi01G020240Bottle gourd (USVL1VR-Ls)cgylsiB283
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.169890Watermelon (97103) v2cgywmbB308
Cucsa.169890Cucurbita maxima (Rimu)cgycmaB0482
Cucsa.169890Cucurbita moschata (Rifu)cgycmoB0485
Cucsa.169890Watermelon (Charleston Gray)cgywcgB304
Cucsa.169890Watermelon (97103) v1cgywmB325
Cucsa.169890Melon (DHL92) v3.6.1cgymedB295
Cucsa.169890Silver-seed gourdcarcgyB0546
Cucsa.169890Cucumber (Chinese Long) v3cgycucB271