BLAST of Cucsa.161140 vs. Swiss-Prot
Match:
MAOX_PHAVU (NADP-dependent malic enzyme OS=Phaseolus vulgaris GN=ME1 PE=2 SV=1)
HSP 1 Score: 997.3 bits (2577), Expect = 7.2e-290
Identity = 497/587 (84.67%), Postives = 537/587 (91.48%), Query Frame = 1
Query: 2 ESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNK 61
E S+KK SNG D+ YGE +ATED LITPWT SVASG SLLRDP YNK
Sbjct: 12 EVSVKKDYSNGGGVRDL----------YGEDSATEDHLITPWTFSVASGCSLLRDPRYNK 71
Query: 62 GLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLF 121
GLAF+E ERDAHYLRGLLPP+V +QELQ KR+MHNLR YEVPLHRY+A+MDLQERNERLF
Sbjct: 72 GLAFTEGERDAHYLRGLLPPSVFNQELQEKRLMHNLRQYEVPLHRYMALMDLQERNERLF 131
Query: 122 YKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQ 181
YKLLIDNV ELLP+VYTPTVGEACQKYGS++RRPQGL+ISLKEKGKILEVLKNWPE++IQ
Sbjct: 132 YKLLIDNVAELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKSIQ 191
Query: 182 VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDE 241
VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPS+CLP+TIDVGTNNE+LLNDE
Sbjct: 192 VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNEKLLNDE 251
Query: 242 FYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVF 301
FYIGLRQ+RA GQEY LDEFM AVK+NYGEKVL+QFEDFANHNAF+LL +YSSSHLVF
Sbjct: 252 FYIGLRQRRATGQEYATFLDEFMRAVKQNYGEKVLVQFEDFANHNAFDLLEKYSSSHLVF 311
Query: 302 NDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTE 361
NDDIQGTASVV+AGLLA+LKL+GGTLADHTFLFLGAGEAGTGIAELIA+E+SKQT AP E
Sbjct: 312 NDDIQGTASVVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIAVEVSKQTKAPVE 371
Query: 362 ETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGK 421
ETRKKIWLVDSKGLIV SR ESLQ FKKPWAH+HE +K L AV+AIKPTVLIG+SG GK
Sbjct: 372 ETRKKIWLVDSKGLIVSSRLESLQQFKKPWAHEHEPVKGLLEAVKAIKPTVLIGSSGAGK 431
Query: 422 TFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGK 481
TFTKEVVE MAS NEKPLILALSNPTSQSECTAEEAYTWS+GRAIFASGSPFDPVEY+GK
Sbjct: 432 TFTKEVVETMASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGK 491
Query: 482 VFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTN 541
+FVPGQANNAYIFPGFGLGLIMSG IRV D+MLLAASEALAAQVS+ENYDKGLIYPPFTN
Sbjct: 492 LFVPGQANNAYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSEENYDKGLIYPPFTN 551
Query: 542 IRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
IRKISANIAAKVAAKAY+LGLAS L RPKDLVK+AESCMYSP YRSY
Sbjct: 552 IRKISANIAAKVAAKAYDLGLASHLKRPKDLVKYAESCMYSPGYRSY 588
BLAST of Cucsa.161140 vs. Swiss-Prot
Match:
MAOC_FLAPR (NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei GN=MODA PE=2 SV=1)
HSP 1 Score: 964.1 bits (2491), Expect = 6.8e-280
Identity = 465/563 (82.59%), Postives = 528/563 (93.78%), Query Frame = 1
Query: 26 EEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVID 85
E+ YGE +ATED ITPW+VSVASGYSLLRDP +NKGLAF+EKERDAHYLRGLLPP V++
Sbjct: 84 EDVYGEDSATEDHFITPWSVSVASGYSLLRDPHHNKGLAFTEKERDAHYLRGLLPPVVVN 143
Query: 86 QELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEAC 145
+LQVK+MMHN+R YEVPL RY AMMDLQERNERLFYKLLI+N+EELLPIVYTPTVGEAC
Sbjct: 144 HDLQVKKMMHNIRQYEVPLQRYQAMMDLQERNERLFYKLLIENIEELLPIVYTPTVGEAC 203
Query: 146 QKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIP 205
QKYG++++ PQGL+ISLK+KGK+LE+LKNWP++ IQVIVVTDGERILGLGDLGCQGMGIP
Sbjct: 204 QKYGTIFKNPQGLYISLKDKGKVLEILKNWPQKKIQVIVVTDGERILGLGDLGCQGMGIP 263
Query: 206 VGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMY 265
VGKLSLYTALGGIRPSACLPITIDVGTNNE++LNDEFYIGLRQ+RA G+EY EL++EFM
Sbjct: 264 VGKLSLYTALGGIRPSACLPITIDVGTNNEKMLNDEFYIGLRQRRASGKEYAELMNEFMS 323
Query: 266 AVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGG 325
AVK+NYGEKVLIQFEDFANHNAF+LL +Y ++HLVFNDDIQGTASVV+AGL++ALKL+GG
Sbjct: 324 AVKQNYGEKVLIQFEDFANHNAFDLLEKYRTTHLVFNDDIQGTASVVLAGLISALKLVGG 383
Query: 326 TLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQ 385
+LADH FLFLGAGEAGTGIAELIALEISKQT AP EETRKKIWLVDSKGLIV+SR +SLQ
Sbjct: 384 SLADHKFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKIWLVDSKGLIVRSRLDSLQ 443
Query: 386 HFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSN 445
HFKKPWAHDHE + AV+AIKPTVLIG+SG G+TFTKEVVEAM+SFNEKP+ILALSN
Sbjct: 444 HFKKPWAHDHEPVNKFLDAVKAIKPTVLIGSSGAGQTFTKEVVEAMSSFNEKPIILALSN 503
Query: 446 PTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSG 505
PTSQSECTAE+AYTWS+GR IFASGSPF PVEY+GKV+V GQ+NNAYIFPGFGLGLI+SG
Sbjct: 504 PTSQSECTAEQAYTWSEGRTIFASGSPFAPVEYNGKVYVSGQSNNAYIFPGFGLGLIISG 563
Query: 506 TIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASR 565
IRVHD+MLLAASEALA QV+QE++D GLIYPPFTNIRKISA+IAAKVAAKAYELGLASR
Sbjct: 564 AIRVHDEMLLAASEALAEQVTQEHFDNGLIYPPFTNIRKISAHIAAKVAAKAYELGLASR 623
Query: 566 LPRPKDLVKFAESCMYSPRYRSY 589
LP+P++LV +AESCMYSP+YR+Y
Sbjct: 624 LPQPENLVAYAESCMYSPKYRNY 646
BLAST of Cucsa.161140 vs. Swiss-Prot
Match:
MAOX_VITVI (NADP-dependent malic enzyme OS=Vitis vinifera PE=2 SV=1)
HSP 1 Score: 957.2 bits (2473), Expect = 8.3e-278
Identity = 472/591 (79.86%), Postives = 536/591 (90.69%), Query Frame = 1
Query: 1 MESSMKKTMSNGSVHMDMEE---IDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDP 60
MES++K SV +D++ + E+ YGE ATEDQL+TPWTVSVASGYSLLRDP
Sbjct: 1 MESTLKDIRDGASV-LDLDPKATVGGGVEDLYGEDFATEDQLVTPWTVSVASGYSLLRDP 60
Query: 61 IYNKGLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERN 120
+NKGLAF++KERDAHYL GLLPP V QELQ +++M+++R Y+VPL +Y+AMMDLQERN
Sbjct: 61 RHNKGLAFNDKERDAHYLCGLLPPVVSTQELQERKLMNSIRQYQVPLQKYMAMMDLQERN 120
Query: 121 ERLFYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPE 180
ERLFYKLLIDNVEELLP+VYTPTVGEACQKYGS++RRPQGL+ISLKEKGKILEVLKNWPE
Sbjct: 121 ERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPE 180
Query: 181 RNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQL 240
R IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPSACLPITIDVGTNNE+L
Sbjct: 181 RRIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKL 240
Query: 241 LNDEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSS 300
L +EFYIGL+Q+RA G+EY E L EFM VK+NYGEKVLIQFEDFANHNAF+LL++Y ++
Sbjct: 241 LANEFYIGLKQRRATGKEYSEFLQEFMSPVKQNYGEKVLIQFEDFANHNAFDLLAKYGTT 300
Query: 301 HLVFNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTG 360
HL FNDDIQGTASVV+AG+++AL+L+GGTLADH FLFLGAGEAGTGIAELIALE+SKQT
Sbjct: 301 HLAFNDDIQGTASVVLAGIVSALRLLGGTLADHKFLFLGAGEAGTGIAELIALEMSKQTK 360
Query: 361 APTEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTS 420
P EETRKKIWLVDSKGLIV SR +SLQ FKKPWAH+HE +KDL AV+ IKPTVLIG+S
Sbjct: 361 CPIEETRKKIWLVDSKGLIVGSRKDSLQQFKKPWAHEHEPVKDLLDAVKVIKPTVLIGSS 420
Query: 421 GVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVE 480
GVGK FTKEV+EAMAS NEKPLILALSNPTSQSECTAEEAYTW+QGRAIFASGSPFDPVE
Sbjct: 421 GVGKAFTKEVIEAMASCNEKPLILALSNPTSQSECTAEEAYTWTQGRAIFASGSPFDPVE 480
Query: 481 YDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYP 540
Y+GK FVPGQANNAYIFPG G+GL++SG IRVHD+MLLAASEALA QV+QEN+DKGLIYP
Sbjct: 481 YNGKTFVPGQANNAYIFPGLGMGLVISGAIRVHDEMLLAASEALARQVTQENFDKGLIYP 540
Query: 541 PFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
PF+NIRKISA+IAA VAAKAYELGLA+RLP+P++LVK+AESCMYSP YRSY
Sbjct: 541 PFSNIRKISAHIAANVAAKAYELGLATRLPQPENLVKYAESCMYSPVYRSY 590
BLAST of Cucsa.161140 vs. Swiss-Prot
Match:
MAOP3_ARATH (NADP-dependent malic enzyme 3 OS=Arabidopsis thaliana GN=NADP-ME3 PE=1 SV=1)
HSP 1 Score: 953.0 bits (2462), Expect = 1.6e-276
Identity = 464/562 (82.56%), Postives = 515/562 (91.64%), Query Frame = 1
Query: 27 EAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQ 86
+ YGE +AT DQL+TPW SVASGY+L+RDP YNKGLAF++KERDAHY+ GLLPP V+ Q
Sbjct: 26 DVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQ 85
Query: 87 ELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEACQ 146
++Q +++MHNLR Y VPL RY+A+MDLQERNERLFYKLLIDNVEELLP+VYTPTVGEACQ
Sbjct: 86 DVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 145
Query: 147 KYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPV 206
KYGS+YRRPQGL+ISLKEKGKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPV
Sbjct: 146 KYGSIYRRPQGLYISLKEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPV 205
Query: 207 GKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYA 266
GKLSLYTALGGIRPSACLPITIDVGTNNE+LLN+EFYIGL+QKRA G+EY E L EFM A
Sbjct: 206 GKLSLYTALGGIRPSACLPITIDVGTNNEKLLNNEFYIGLKQKRANGEEYAEFLQEFMCA 265
Query: 267 VKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGT 326
VK+NYGEKVL+QFEDFANH+AFELLS+Y SSHLVFNDDIQGTASVV+AGL+AA K++G +
Sbjct: 266 VKQNYGEKVLVQFEDFANHHAFELLSKYCSSHLVFNDDIQGTASVVLAGLIAAQKVLGKS 325
Query: 327 LADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQH 386
LADHTFLFLGAGEAGTGIAELIAL+ISK+TG P +ETRKKIWLVDSKGLIV R ESLQH
Sbjct: 326 LADHTFLFLGAGEAGTGIAELIALKISKETGKPIDETRKKIWLVDSKGLIVSERKESLQH 385
Query: 387 FKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNP 446
FK+PWAHDH+ +K+L AV AIKPTVLIGTSGVGKTFTKEVVEAMA+ NEKPLILALSNP
Sbjct: 386 FKQPWAHDHKPVKELLAAVNAIKPTVLIGTSGVGKTFTKEVVEAMATLNEKPLILALSNP 445
Query: 447 TSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGT 506
TSQ+ECTAEEAYTW++GRAIFASGSPFDPV+YDGK F PGQANN YIFPG GLGLIMSG
Sbjct: 446 TSQAECTAEEAYTWTKGRAIFASGSPFDPVQYDGKKFTPGQANNCYIFPGLGLGLIMSGA 505
Query: 507 IRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRL 566
IRV DDMLLAASEALA+QV++EN+ GLIYPPF NIRKISANIAA V AK YELGLAS L
Sbjct: 506 IRVRDDMLLAASEALASQVTEENFANGLIYPPFANIRKISANIAASVGAKTYELGLASNL 565
Query: 567 PRPKDLVKFAESCMYSPRYRSY 589
PRPKDLVK AESCMYSP YR++
Sbjct: 566 PRPKDLVKMAESCMYSPVYRNF 587
BLAST of Cucsa.161140 vs. Swiss-Prot
Match:
MAOX_POPTR (NADP-dependent malic enzyme OS=Populus trichocarpa PE=2 SV=3)
HSP 1 Score: 950.3 bits (2455), Expect = 1.0e-275
Identity = 462/591 (78.17%), Postives = 535/591 (90.52%), Query Frame = 1
Query: 1 MESSMKKTMSNGSVHMDMEE---IDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDP 60
MES++K+ M +G+ +DM+ + E+ YGE ATEDQL+TPWT+SVASGY+LLRDP
Sbjct: 1 MESTLKE-MRDGASVLDMDPKSTVGGGVEDVYGEDRATEDQLVTPWTISVASGYTLLRDP 60
Query: 61 IYNKGLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERN 120
+NKGLAF+EKERDAHYLRGLLPP I Q+LQ K++M+ +R Y++PL +Y AMM+L+ERN
Sbjct: 61 HHNKGLAFTEKERDAHYLRGLLPPTTISQQLQEKKLMNTIRQYQLPLQKYTAMMELEERN 120
Query: 121 ERLFYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPE 180
ERLFYKLLIDNVEELLP+VYTPTVGEACQKYGS+++RPQGL+ISLKEKGK+L+VLKNWP+
Sbjct: 121 ERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYISLKEKGKVLDVLKNWPQ 180
Query: 181 RNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQL 240
++IQVIVVTDGERILGLGDLGCQG+GIPVGKLSLYTALGG+RPSACLP+TIDVGTNNEQL
Sbjct: 181 KSIQVIVVTDGERILGLGDLGCQGIGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEQL 240
Query: 241 LNDEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSS 300
L DEFYIGLRQ+RA GQEY ELL EFM AVK+NYGEKVLIQFEDFANHNAF+LL++Y ++
Sbjct: 241 LKDEFYIGLRQRRATGQEYSELLHEFMTAVKQNYGEKVLIQFEDFANHNAFDLLAKYGTT 300
Query: 301 HLVFNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTG 360
HLVFNDDIQGTA+VV+AGL++ALKL+GG+LADHTFLFLGAGEAGTGIAELIALE+S+++
Sbjct: 301 HLVFNDDIQGTAAVVLAGLISALKLLGGSLADHTFLFLGAGEAGTGIAELIALEMSRRSK 360
Query: 361 APTEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTS 420
P EETRKKIWL DSKGLIV SR ESLQHFKKPWAH+HE +K L V+AIKP VLIGTS
Sbjct: 361 TPLEETRKKIWLTDSKGLIVSSRKESLQHFKKPWAHEHEPVKGLLEVVKAIKPIVLIGTS 420
Query: 421 GVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVE 480
GVGKTFTKEV+EAMASFNEKPLILALSNPTSQSECTA+EAYTW++G+AIFASGSPFDPVE
Sbjct: 421 GVGKTFTKEVIEAMASFNEKPLILALSNPTSQSECTAQEAYTWTKGKAIFASGSPFDPVE 480
Query: 481 YDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYP 540
Y+GKVFVPGQ+NNAYIFPG GLGL++SG IRVHDDMLLAA+EALA Q+ +E KGLIYP
Sbjct: 481 YEGKVFVPGQSNNAYIFPGLGLGLVISGAIRVHDDMLLAAAEALAGQIKEEYLAKGLIYP 540
Query: 541 PFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
P +NIRKIS IAA VAAKAYELGLA+RLPRP++LVK AESCMYSP YR Y
Sbjct: 541 PLSNIRKISVQIAANVAAKAYELGLATRLPRPENLVKHAESCMYSPAYRYY 590
BLAST of Cucsa.161140 vs. TrEMBL
Match:
A0A0A0LLH9_CUCSA (Malic enzyme OS=Cucumis sativus GN=Csa_2G373430 PE=3 SV=1)
HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 587/589 (99.66%), Postives = 588/589 (99.83%), Query Frame = 1
Query: 1 MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 1 MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
Query: 61 KGLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
KGLAFSEKERDAHYLRGLLPPAVIDQELQ +RMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 61 KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
Query: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
Query: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
Query: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV
Sbjct: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
Query: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT
Sbjct: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
Query: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG
Sbjct: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
Query: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG
Sbjct: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
Query: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT
Sbjct: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
Query: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 590
NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 589
BLAST of Cucsa.161140 vs. TrEMBL
Match:
X2C4W9_9FABA (Malic enzyme OS=Stylosanthes guianensis PE=2 SV=1)
HSP 1 Score: 1010.7 bits (2612), Expect = 7.0e-292
Identity = 500/584 (85.62%), Postives = 541/584 (92.64%), Query Frame = 1
Query: 5 MKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLA 64
M T+ NG V +D + YGE ATEDQLITPW SV+SG +LLRDP YNKGLA
Sbjct: 1 MSSTLVNGGVEEASATVDGGVRDFYGEERATEDQLITPWNFSVSSGSTLLRDPRYNKGLA 60
Query: 65 FSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKL 124
F+EKERDAHYLRGLLPPAV +QELQ KR+M+NLR YEVPLHRY+A+MDLQERNERLFYK+
Sbjct: 61 FTEKERDAHYLRGLLPPAVFNQELQEKRLMYNLRQYEVPLHRYMALMDLQERNERLFYKV 120
Query: 125 LIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIV 184
LIDNVEELLP+VYTPTVGEACQKYGS++RRPQGL+ISLKEKGKILEVLKNWPE+NIQVIV
Sbjct: 121 LIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKNIQVIV 180
Query: 185 VTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYI 244
VTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPS+CLPITIDVGTNNE+LLNDEFYI
Sbjct: 181 VTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLPITIDVGTNNEKLLNDEFYI 240
Query: 245 GLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDD 304
GLRQKRA G+EY ELLDEFM AVKKNYGEKVLIQFEDFANHNAF+LL++YSSSHLVFNDD
Sbjct: 241 GLRQKRATGKEYAELLDEFMCAVKKNYGEKVLIQFEDFANHNAFDLLAKYSSSHLVFNDD 300
Query: 305 IQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETR 364
IQGTASVV+AGLLA+LKL+GG LADHTFLFLGAGEAGTGIAELIALEISKQT AP EETR
Sbjct: 301 IQGTASVVLAGLLASLKLVGGNLADHTFLFLGAGEAGTGIAELIALEISKQTKAPVEETR 360
Query: 365 KKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFT 424
KKIWLVDSKGLIV SR SLQHFKKPWAH+HE +K+L AV+AIKPTVLIG+SGVGKTFT
Sbjct: 361 KKIWLVDSKGLIVSSRLGSLQHFKKPWAHEHEPVKELVDAVKAIKPTVLIGSSGVGKTFT 420
Query: 425 KEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFV 484
KEVVE MAS N+KPLILALSNPTSQSECTAEEAYTWS+G+AIFASGSPFDPVEY+GKVFV
Sbjct: 421 KEVVETMASLNKKPLILALSNPTSQSECTAEEAYTWSKGQAIFASGSPFDPVEYEGKVFV 480
Query: 485 PGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRK 544
PGQANNAYIFPGFGLGLIMSG IRV D+MLLAASEALAAQVSQENYDKGLIYPPFTNIRK
Sbjct: 481 PGQANNAYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSQENYDKGLIYPPFTNIRK 540
Query: 545 ISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
ISA+IAA VAAKAYELGLAS LP+PKDLVK+AESCMYSP YR+Y
Sbjct: 541 ISAHIAANVAAKAYELGLASNLPQPKDLVKYAESCMYSPGYRNY 584
BLAST of Cucsa.161140 vs. TrEMBL
Match:
V7AUA6_PHAVU (Malic enzyme OS=Phaseolus vulgaris GN=PHAVU_009G111500g PE=3 SV=1)
HSP 1 Score: 1007.7 bits (2604), Expect = 5.9e-291
Identity = 502/587 (85.52%), Postives = 541/587 (92.16%), Query Frame = 1
Query: 2 ESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNK 61
E S+KK SNG D+ YGE +ATED LITPWT SVASG SLLRDP YNK
Sbjct: 38 EVSVKKDYSNGGGVRDL----------YGEDSATEDHLITPWTFSVASGCSLLRDPRYNK 97
Query: 62 GLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLF 121
GLAF+E ERDAHYLRGLLPP+V +QELQ KR+MHNLR YEVPLHRY+A+MDLQERNERLF
Sbjct: 98 GLAFTEGERDAHYLRGLLPPSVFNQELQEKRLMHNLRQYEVPLHRYMALMDLQERNERLF 157
Query: 122 YKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQ 181
YKLLIDNVEELLP+VYTPTVGEACQKYGS++RRPQGL+ISLKEKGKILEVLKNWPE++IQ
Sbjct: 158 YKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKSIQ 217
Query: 182 VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDE 241
VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPS+CLP+TIDVGTNNE+LLNDE
Sbjct: 218 VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNEKLLNDE 277
Query: 242 FYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVF 301
FYIGLRQ+RA GQEY ELLDEFM AVK+NYGEKVL+QFEDFANHNAF+LL +YSSSHLVF
Sbjct: 278 FYIGLRQRRATGQEYAELLDEFMRAVKQNYGEKVLVQFEDFANHNAFDLLEKYSSSHLVF 337
Query: 302 NDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTE 361
NDDIQGTASVV+AGLLA+LKL+GGTLADHTFLFLGAGEAGTGIAELIALE+SKQT AP E
Sbjct: 338 NDDIQGTASVVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTKAPVE 397
Query: 362 ETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGK 421
ETRKKIWLVDSKGLIV SR ESLQ FKKPWAH+HE +K L AV+AIKPTVLIG+SG GK
Sbjct: 398 ETRKKIWLVDSKGLIVSSRLESLQQFKKPWAHEHEPVKGLLEAVKAIKPTVLIGSSGAGK 457
Query: 422 TFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGK 481
TFTKEVVE MAS NEKPLILALSNPTSQSECTAEEAYTWS+GRAIFASGSPFDPVEY+GK
Sbjct: 458 TFTKEVVETMASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGK 517
Query: 482 VFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTN 541
+FVPGQANNAYIFPGFGLGLIMSG IRV D+MLLAASEALAAQVS+ENYDKGLIYPPFTN
Sbjct: 518 LFVPGQANNAYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSEENYDKGLIYPPFTN 577
Query: 542 IRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
IRKISANIAAKVAAKAY+LGLAS LPRPKDLVK+AESCMYSP YRSY
Sbjct: 578 IRKISANIAAKVAAKAYDLGLASHLPRPKDLVKYAESCMYSPGYRSY 614
BLAST of Cucsa.161140 vs. TrEMBL
Match:
V7AXA3_PHAVU (Malic enzyme OS=Phaseolus vulgaris GN=PHAVU_009G111500g PE=3 SV=1)
HSP 1 Score: 1007.7 bits (2604), Expect = 5.9e-291
Identity = 502/587 (85.52%), Postives = 541/587 (92.16%), Query Frame = 1
Query: 2 ESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNK 61
E S+KK SNG D+ YGE +ATED LITPWT SVASG SLLRDP YNK
Sbjct: 12 EVSVKKDYSNGGGVRDL----------YGEDSATEDHLITPWTFSVASGCSLLRDPRYNK 71
Query: 62 GLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLF 121
GLAF+E ERDAHYLRGLLPP+V +QELQ KR+MHNLR YEVPLHRY+A+MDLQERNERLF
Sbjct: 72 GLAFTEGERDAHYLRGLLPPSVFNQELQEKRLMHNLRQYEVPLHRYMALMDLQERNERLF 131
Query: 122 YKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQ 181
YKLLIDNVEELLP+VYTPTVGEACQKYGS++RRPQGL+ISLKEKGKILEVLKNWPE++IQ
Sbjct: 132 YKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKSIQ 191
Query: 182 VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDE 241
VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPS+CLP+TIDVGTNNE+LLNDE
Sbjct: 192 VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNEKLLNDE 251
Query: 242 FYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVF 301
FYIGLRQ+RA GQEY ELLDEFM AVK+NYGEKVL+QFEDFANHNAF+LL +YSSSHLVF
Sbjct: 252 FYIGLRQRRATGQEYAELLDEFMRAVKQNYGEKVLVQFEDFANHNAFDLLEKYSSSHLVF 311
Query: 302 NDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTE 361
NDDIQGTASVV+AGLLA+LKL+GGTLADHTFLFLGAGEAGTGIAELIALE+SKQT AP E
Sbjct: 312 NDDIQGTASVVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTKAPVE 371
Query: 362 ETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGK 421
ETRKKIWLVDSKGLIV SR ESLQ FKKPWAH+HE +K L AV+AIKPTVLIG+SG GK
Sbjct: 372 ETRKKIWLVDSKGLIVSSRLESLQQFKKPWAHEHEPVKGLLEAVKAIKPTVLIGSSGAGK 431
Query: 422 TFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGK 481
TFTKEVVE MAS NEKPLILALSNPTSQSECTAEEAYTWS+GRAIFASGSPFDPVEY+GK
Sbjct: 432 TFTKEVVETMASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGK 491
Query: 482 VFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTN 541
+FVPGQANNAYIFPGFGLGLIMSG IRV D+MLLAASEALAAQVS+ENYDKGLIYPPFTN
Sbjct: 492 LFVPGQANNAYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSEENYDKGLIYPPFTN 551
Query: 542 IRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
IRKISANIAAKVAAKAY+LGLAS LPRPKDLVK+AESCMYSP YRSY
Sbjct: 552 IRKISANIAAKVAAKAYDLGLASHLPRPKDLVKYAESCMYSPGYRSY 588
BLAST of Cucsa.161140 vs. TrEMBL
Match:
A0A072VD34_MEDTR (Malic enzyme OS=Medicago truncatula GN=MTR_3g110330 PE=3 SV=1)
HSP 1 Score: 1007.3 bits (2603), Expect = 7.8e-291
Identity = 494/563 (87.74%), Postives = 535/563 (95.03%), Query Frame = 1
Query: 26 EEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVID 85
++ YGE +ATEDQLITPW SVASG +LLRDP YNKGLAF+EKERDAHY+RGLLPPAV
Sbjct: 15 KDLYGEDSATEDQLITPWNFSVASGCTLLRDPRYNKGLAFTEKERDAHYVRGLLPPAVFT 74
Query: 86 QELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEAC 145
QELQ KR+MHNLR Y+VPLH+YIA+MDLQERNERLFYKL+IDNVEELLP+VYTPTVGEAC
Sbjct: 75 QELQEKRLMHNLRQYDVPLHKYIALMDLQERNERLFYKLMIDNVEELLPVVYTPTVGEAC 134
Query: 146 QKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIP 205
QKYGS+YRRPQGL+ISLKEKGKILEVLKNWPE+ IQVIVVTDGERILGLGDLGCQGMGIP
Sbjct: 135 QKYGSIYRRPQGLYISLKEKGKILEVLKNWPEKTIQVIVVTDGERILGLGDLGCQGMGIP 194
Query: 206 VGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMY 265
VGKLSLYTALGG+RPS+CLPITIDVGTNNE+LLNDEFYIGLRQKRA G+EY ELL+EFM+
Sbjct: 195 VGKLSLYTALGGVRPSSCLPITIDVGTNNEKLLNDEFYIGLRQKRATGKEYAELLEEFMH 254
Query: 266 AVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGG 325
AVK+NYGEKVL+QFEDFANHNAF+LL +YSSSHLVFNDDIQGTASVV+AGLLA+LKL+GG
Sbjct: 255 AVKQNYGEKVLVQFEDFANHNAFDLLDKYSSSHLVFNDDIQGTASVVLAGLLASLKLVGG 314
Query: 326 TLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQ 385
TLADHTFLFLGAGEAGTGIAELIALEISKQT AP EETRKKIWLVDSKGLIV SR +SLQ
Sbjct: 315 TLADHTFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKIWLVDSKGLIVSSRLQSLQ 374
Query: 386 HFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSN 445
HFKKPWAH+HE +K+L AV+AIKPTVLIG+SGVGKTFTKEVVEAMAS NEKPLILALSN
Sbjct: 375 HFKKPWAHEHEPVKELLNAVKAIKPTVLIGSSGVGKTFTKEVVEAMASLNEKPLILALSN 434
Query: 446 PTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSG 505
PTSQSECTAEEAYTWS+G+AIFASGSPFDPVEY+GKVFVPGQ+NNAYIFPGFGLGLI+SG
Sbjct: 435 PTSQSECTAEEAYTWSKGKAIFASGSPFDPVEYEGKVFVPGQSNNAYIFPGFGLGLIISG 494
Query: 506 TIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASR 565
IRV D+MLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAA VAAK YELGLAS
Sbjct: 495 AIRVRDEMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAASVAAKTYELGLASH 554
Query: 566 LPRPKDLVKFAESCMYSPRYRSY 589
LPRPKDLVK+AESCMYSP YRSY
Sbjct: 555 LPRPKDLVKYAESCMYSPGYRSY 577
BLAST of Cucsa.161140 vs. TAIR10
Match:
AT5G25880.1 (AT5G25880.1 NADP-malic enzyme 3)
HSP 1 Score: 953.0 bits (2462), Expect = 8.8e-278
Identity = 464/562 (82.56%), Postives = 515/562 (91.64%), Query Frame = 1
Query: 27 EAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQ 86
+ YGE +AT DQL+TPW SVASGY+L+RDP YNKGLAF++KERDAHY+ GLLPP V+ Q
Sbjct: 26 DVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQ 85
Query: 87 ELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEACQ 146
++Q +++MHNLR Y VPL RY+A+MDLQERNERLFYKLLIDNVEELLP+VYTPTVGEACQ
Sbjct: 86 DVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 145
Query: 147 KYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPV 206
KYGS+YRRPQGL+ISLKEKGKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPV
Sbjct: 146 KYGSIYRRPQGLYISLKEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPV 205
Query: 207 GKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYA 266
GKLSLYTALGGIRPSACLPITIDVGTNNE+LLN+EFYIGL+QKRA G+EY E L EFM A
Sbjct: 206 GKLSLYTALGGIRPSACLPITIDVGTNNEKLLNNEFYIGLKQKRANGEEYAEFLQEFMCA 265
Query: 267 VKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGT 326
VK+NYGEKVL+QFEDFANH+AFELLS+Y SSHLVFNDDIQGTASVV+AGL+AA K++G +
Sbjct: 266 VKQNYGEKVLVQFEDFANHHAFELLSKYCSSHLVFNDDIQGTASVVLAGLIAAQKVLGKS 325
Query: 327 LADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQH 386
LADHTFLFLGAGEAGTGIAELIAL+ISK+TG P +ETRKKIWLVDSKGLIV R ESLQH
Sbjct: 326 LADHTFLFLGAGEAGTGIAELIALKISKETGKPIDETRKKIWLVDSKGLIVSERKESLQH 385
Query: 387 FKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNP 446
FK+PWAHDH+ +K+L AV AIKPTVLIGTSGVGKTFTKEVVEAMA+ NEKPLILALSNP
Sbjct: 386 FKQPWAHDHKPVKELLAAVNAIKPTVLIGTSGVGKTFTKEVVEAMATLNEKPLILALSNP 445
Query: 447 TSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGT 506
TSQ+ECTAEEAYTW++GRAIFASGSPFDPV+YDGK F PGQANN YIFPG GLGLIMSG
Sbjct: 446 TSQAECTAEEAYTWTKGRAIFASGSPFDPVQYDGKKFTPGQANNCYIFPGLGLGLIMSGA 505
Query: 507 IRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRL 566
IRV DDMLLAASEALA+QV++EN+ GLIYPPF NIRKISANIAA V AK YELGLAS L
Sbjct: 506 IRVRDDMLLAASEALASQVTEENFANGLIYPPFANIRKISANIAASVGAKTYELGLASNL 565
Query: 567 PRPKDLVKFAESCMYSPRYRSY 589
PRPKDLVK AESCMYSP YR++
Sbjct: 566 PRPKDLVKMAESCMYSPVYRNF 587
BLAST of Cucsa.161140 vs. TAIR10
Match:
AT5G11670.1 (AT5G11670.1 NADP-malic enzyme 2)
HSP 1 Score: 943.7 bits (2438), Expect = 5.3e-275
Identity = 461/562 (82.03%), Postives = 515/562 (91.64%), Query Frame = 1
Query: 27 EAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQ 86
+ YGE +AT DQL+TPW SVASGY+L+RDP YNKGLAF++KERDAHYL GLLPP ++ Q
Sbjct: 26 DVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYLTGLLPPVILSQ 85
Query: 87 ELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEACQ 146
++Q +++MHNLR Y VPL RY+A+MDLQERNERLFYKLLIDNVEELLP+VYTPTVGEACQ
Sbjct: 86 DVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 145
Query: 147 KYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPV 206
KYGS++R+PQGL+ISL EKGKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPV
Sbjct: 146 KYGSIFRKPQGLYISLNEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPV 205
Query: 207 GKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYA 266
GKLSLYTALGGIRPSACLPITIDVGTNNE+LLNDEFYIGL+Q+RA GQEY E L EFM A
Sbjct: 206 GKLSLYTALGGIRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGQEYAEFLHEFMCA 265
Query: 267 VKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGT 326
VK+NYGEKVL+QFEDFANHNAF+LLS+YS SHLVFNDDIQGTASVV+AGL+AA K++G
Sbjct: 266 VKQNYGEKVLVQFEDFANHNAFDLLSKYSDSHLVFNDDIQGTASVVLAGLIAAQKVLGKK 325
Query: 327 LADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQH 386
LADHTFLFLGAGEAGTGIAELIAL+ISK+TGAP ETRKKIWLVDSKGLIV SR ESLQH
Sbjct: 326 LADHTFLFLGAGEAGTGIAELIALKISKETGAPITETRKKIWLVDSKGLIVSSRKESLQH 385
Query: 387 FKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNP 446
FK+PWAH+H+ +KDL GAV AIKPTVLIGTSGVG+TFTKEVVEAMA+ NEKPLILALSNP
Sbjct: 386 FKQPWAHEHKPVKDLIGAVNAIKPTVLIGTSGVGQTFTKEVVEAMATNNEKPLILALSNP 445
Query: 447 TSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGT 506
TSQ+ECTAE+AYTW++GRAIF SGSPFDPV YDGK ++PGQANN YIFPG GLGLIMSG
Sbjct: 446 TSQAECTAEQAYTWTKGRAIFGSGSPFDPVVYDGKTYLPGQANNCYIFPGLGLGLIMSGA 505
Query: 507 IRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRL 566
IRV DDMLLAASEALAAQV++E+Y GLIYPPF+NIR+ISANIAA VAAK Y+LGLAS L
Sbjct: 506 IRVRDDMLLAASEALAAQVTEEHYANGLIYPPFSNIREISANIAACVAAKTYDLGLASNL 565
Query: 567 PRPKDLVKFAESCMYSPRYRSY 589
PR KDLVKFAES MYSP YR+Y
Sbjct: 566 PRAKDLVKFAESSMYSPVYRNY 587
BLAST of Cucsa.161140 vs. TAIR10
Match:
AT1G79750.1 (AT1G79750.1 NADP-malic enzyme 4)
HSP 1 Score: 941.0 bits (2431), Expect = 3.5e-274
Identity = 457/563 (81.17%), Postives = 518/563 (92.01%), Query Frame = 1
Query: 26 EEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVID 85
++ YGE ATED ITPW++SVASGY+LLRDP +NKGLAFS +ERDAHYLRGLLPP VI
Sbjct: 83 QDVYGEDAATEDMPITPWSLSVASGYTLLRDPHHNKGLAFSHRERDAHYLRGLLPPTVIS 142
Query: 86 QELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEAC 145
Q+LQVK++MH LR Y+VPL +Y+AMMDLQE NERLFYKLLID+VEELLP++YTPTVGEAC
Sbjct: 143 QDLQVKKIMHTLRQYQVPLQKYMAMMDLQETNERLFYKLLIDHVEELLPVIYTPTVGEAC 202
Query: 146 QKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIP 205
QKYGS++ RPQGLFISLKEKGKI EVL+NWPE+NIQVIVVTDGERILGLGDLGCQGMGIP
Sbjct: 203 QKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKNIQVIVVTDGERILGLGDLGCQGMGIP 262
Query: 206 VGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMY 265
VGKLSLYTALGG+RPSACLP+TIDVGTNNE+LLNDEFYIGLRQ+RA G+EY EL+ EFM
Sbjct: 263 VGKLSLYTALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQRRATGEEYSELMHEFMT 322
Query: 266 AVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGG 325
AVK+NYGEKV+IQFEDFANHNAF+LL++Y ++HLVFNDDIQGTASVV+AGL+AAL+ +GG
Sbjct: 323 AVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTHLVFNDDIQGTASVVLAGLIAALRFVGG 382
Query: 326 TLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQ 385
+L+DH FLFLGAGEAGTGIAELIALEISK++ P EE RK IWLVDSKGLIV SR ES+Q
Sbjct: 383 SLSDHRFLFLGAGEAGTGIAELIALEISKKSHIPLEEARKNIWLVDSKGLIVSSRKESIQ 442
Query: 386 HFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSN 445
HFKKPWAHDHE I++L AV+AIKPTVLIGTSGVG+TFT++VVE MA NEKP+IL+LSN
Sbjct: 443 HFKKPWAHDHEPIRELVDAVKAIKPTVLIGTSGVGQTFTQDVVETMAKLNEKPIILSLSN 502
Query: 446 PTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSG 505
PTSQSECTAEEAYTWSQGRAIFASGSPF PVEY+GK FVPGQANNAYIFPGFGLGLIMSG
Sbjct: 503 PTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKTFVPGQANNAYIFPGFGLGLIMSG 562
Query: 506 TIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASR 565
TIRVHDDMLLAASEALA ++ +E+Y+KG+IYPPF NIRKISA IAAKVAAKAYELGLA+R
Sbjct: 563 TIRVHDDMLLAASEALAEELMEEHYEKGMIYPPFRNIRKISARIAAKVAAKAYELGLATR 622
Query: 566 LPRPKDLVKFAESCMYSPRYRSY 589
LP+PK+L + AES MYSP YRSY
Sbjct: 623 LPQPKELEQCAESSMYSPSYRSY 645
BLAST of Cucsa.161140 vs. TAIR10
Match:
AT2G19900.1 (AT2G19900.1 NADP-malic enzyme 1)
HSP 1 Score: 931.8 bits (2407), Expect = 2.1e-271
Identity = 454/568 (79.93%), Postives = 518/568 (91.20%), Query Frame = 1
Query: 21 IDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLP 80
+D + YGE +AT + ITPW++SV+SGYSLLRDP YNKGLAF+EKERD HYLRGLLP
Sbjct: 13 VDGGVVDVYGEDSATIEHNITPWSLSVSSGYSLLRDPRYNKGLAFTEKERDTHYLRGLLP 72
Query: 81 PAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPT 140
P V+DQ+LQ KR+++N+R Y+ PL +Y+A+ +LQERNERLFYKLLIDNVEELLPIVYTPT
Sbjct: 73 PVVLDQKLQEKRLLNNIRQYQFPLQKYMALTELQERNERLFYKLLIDNVEELLPIVYTPT 132
Query: 141 VGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQ 200
VGEACQK+GS++RRPQGLFISLK+KGKIL+VLKNWPERNIQVIVVTDGERILGLGDLGCQ
Sbjct: 133 VGEACQKFGSIFRRPQGLFISLKDKGKILDVLKNWPERNIQVIVVTDGERILGLGDLGCQ 192
Query: 201 GMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELL 260
GMGIPVGKL+LY+ALGG+RPSACLP+TIDVGTNNE+LLNDEFYIGLRQKRA GQEY ELL
Sbjct: 193 GMGIPVGKLALYSALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQKRATGQEYSELL 252
Query: 261 DEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAAL 320
+EFM AVK+NYGEKVLIQFEDFANHNAFELL++YS +HLVFNDDIQGTASVV+AGL++A
Sbjct: 253 NEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYSDTHLVFNDDIQGTASVVLAGLVSAQ 312
Query: 321 KLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSR 380
KL LA+HTFLFLGAGEAGTGIAELIAL +SKQ A EE+RKKIWLVDSKGLIV SR
Sbjct: 313 KLTNSPLAEHTFLFLGAGEAGTGIAELIALYMSKQMNASVEESRKKIWLVDSKGLIVNSR 372
Query: 381 FESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLI 440
+SLQ FKKPWAH+HE +KDL GA++AIKPTVLIG+SGVG++FTKEV+EAM+S NE+PLI
Sbjct: 373 KDSLQDFKKPWAHEHEPVKDLLGAIKAIKPTVLIGSSGVGRSFTKEVIEAMSSINERPLI 432
Query: 441 LALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLG 500
+ALSNPT+QSECTAEEAYTWS+GRAIFASGSPFDPVEY+GKVFV QANNAYIFPGFGLG
Sbjct: 433 MALSNPTTQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKVFVSTQANNAYIFPGFGLG 492
Query: 501 LIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYEL 560
L++SG IRVHDDMLLAA+EALA QVS+ENY+KG+IYP F++IRKISA IAA VA KAYEL
Sbjct: 493 LVISGAIRVHDDMLLAAAEALAGQVSKENYEKGMIYPSFSSIRKISAQIAANVATKAYEL 552
Query: 561 GLASRLPRPKDLVKFAESCMYSPRYRSY 589
GLA RLPRPKD+VK AES MYSP YR Y
Sbjct: 553 GLAGRLPRPKDIVKCAESSMYSPTYRLY 580
BLAST of Cucsa.161140 vs. TAIR10
Match:
AT2G13560.1 (AT2G13560.1 NAD-dependent malic enzyme 1)
HSP 1 Score: 438.3 bits (1126), Expect = 7.3e-123
Identity = 246/578 (42.56%), Postives = 357/578 (61.76%), Query Frame = 1
Query: 44 TVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVP 103
T+ G +L DP +NKG AF+ ER+ LRGLLPP V+D E Q+ R M +L+ E
Sbjct: 40 TIVHKQGLDILHDPWFNKGTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQ 99
Query: 104 ----------LHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEACQKYGSVYR 163
L ++ + L +RNE ++YK+LI+N+EE PIVYTPTVG CQ Y ++R
Sbjct: 100 ARDGPSDPNALAKWRILNRLHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFR 159
Query: 164 RPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 223
RP+G++ S +++G+++ ++ NWP + +IVVTDG RILGLGDLG G+GI VGKL LY
Sbjct: 160 RPRGMYFSAEDRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVHGIGIAVGKLDLYV 219
Query: 224 ALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGE 283
A GI P LP+ IDVGTNNE+L ND Y+GL+Q+R +Y++++DEFM AV +
Sbjct: 220 AAAGINPQRVLPVMIDVGTNNEKLRNDPMYLGLQQRRLEDDDYIDVIDEFMEAVYTRW-P 279
Query: 284 KVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLADH--- 343
V++QFEDF + AF+LL RY ++ +FNDD+QGTA V +AGLL A++ G + D
Sbjct: 280 HVIVQFEDFQSKWAFKLLQRYRCTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKM 339
Query: 344 TFLFLGAGEAGTGIAELIALEISKQTG---APTEETRKKIWLVDSKGLIVQSRFESLQHF 403
+ GAG AG G+ +++ G + + + W+VD++GLI + R E++
Sbjct: 340 KIVVAGAGSAGIGVLNAARKTMARMLGNTETAFDSAQSQFWVVDAQGLITEGR-ENIDPE 399
Query: 404 KKPWAH-----DHESIKD---LYGAVQAIKPTVLIGTSGVGKTFTKEVVEAM-ASFNEKP 463
+P+A + + +K+ L V+ +KP VL+G S VG F+KEV+EAM S + +P
Sbjct: 400 AQPFARKTKEMERQGLKEGATLVEVVREVKPDVLLGLSAVGGLFSKEVLEAMKGSTSTRP 459
Query: 464 LILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEY-DGKVFVPGQANNAYIFPGF 523
I A+SNPT +ECT ++A++ IFASGSPF VE+ +G V Q NN Y+FPG
Sbjct: 460 AIFAMSNPTKNAECTPQDAFSILGENMIFASGSPFKNVEFGNGHVGHCNQGNNMYLFPGI 519
Query: 524 GLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKA 583
GLG ++SG V D ML AASE LAA +S+E +G+IYPP + IR I+ IAA V +A
Sbjct: 520 GLGTLLSGAPIVSDGMLQAASECLAAYMSEEEVLEGIIYPPISRIRDITKRIAAAVIKEA 579
Query: 584 YELGL--------ASRLPR--PKDLVKFAESCMYSPRY 586
E L A + + + L+++ E+ M++P Y
Sbjct: 580 IEEDLVEGYREMDAREIQKLDEEGLMEYVENNMWNPEY 615
BLAST of Cucsa.161140 vs. NCBI nr
Match:
gi|778672002|ref|XP_011649727.1| (PREDICTED: NADP-dependent malic enzyme [Cucumis sativus])
HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 587/589 (99.66%), Postives = 588/589 (99.83%), Query Frame = 1
Query: 1 MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 28 MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 87
Query: 61 KGLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
KGLAFSEKERDAHYLRGLLPPAVIDQELQ +RMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 88 KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 147
Query: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 148 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 207
Query: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 208 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 267
Query: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV
Sbjct: 268 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 327
Query: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT
Sbjct: 328 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 387
Query: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG
Sbjct: 388 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 447
Query: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG
Sbjct: 448 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 507
Query: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT
Sbjct: 508 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 567
Query: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 590
NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 568 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 616
BLAST of Cucsa.161140 vs. NCBI nr
Match:
gi|700207681|gb|KGN62800.1| (hypothetical protein Csa_2G373430 [Cucumis sativus])
HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 587/589 (99.66%), Postives = 588/589 (99.83%), Query Frame = 1
Query: 1 MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 1 MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
Query: 61 KGLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
KGLAFSEKERDAHYLRGLLPPAVIDQELQ +RMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 61 KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
Query: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
Query: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
Query: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV
Sbjct: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
Query: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT
Sbjct: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
Query: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG
Sbjct: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
Query: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG
Sbjct: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
Query: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT
Sbjct: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
Query: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 590
NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 589
BLAST of Cucsa.161140 vs. NCBI nr
Match:
gi|659088473|ref|XP_008445000.1| (PREDICTED: NADP-dependent malic enzyme [Cucumis melo])
HSP 1 Score: 1156.4 bits (2990), Expect = 0.0e+00
Identity = 577/589 (97.96%), Postives = 586/589 (99.49%), Query Frame = 1
Query: 1 MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
MESSMKKTMSNGSVHM+MEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 1 MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
Query: 61 KGLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
KGLAFSEKER+AHYLRGLLPPA++DQELQ +RMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 61 KGLAFSEKERNAHYLRGLLPPALLDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
Query: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
FYKLLIDNVEELLP+VYTPTVGEACQKYGS+YRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 121 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKGKILEVLKNWPERNI 180
Query: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
Query: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV
Sbjct: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
Query: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
FNDDIQGTASVVVAGLLAALKL+GGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP
Sbjct: 301 FNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPI 360
Query: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG
Sbjct: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
Query: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG
Sbjct: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
Query: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
K FVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT
Sbjct: 481 KAFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
Query: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 590
NIRKISANIAA+VAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 541 NIRKISANIAAEVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 589
BLAST of Cucsa.161140 vs. NCBI nr
Match:
gi|468861715|gb|AGH32501.1| (malic enzyme [Stylosanthes guianensis])
HSP 1 Score: 1010.7 bits (2612), Expect = 1.0e-291
Identity = 500/584 (85.62%), Postives = 541/584 (92.64%), Query Frame = 1
Query: 5 MKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLA 64
M T+ NG V +D + YGE ATEDQLITPW SV+SG +LLRDP YNKGLA
Sbjct: 1 MSSTLVNGGVEEASATVDGGVRDFYGEERATEDQLITPWNFSVSSGSTLLRDPRYNKGLA 60
Query: 65 FSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKL 124
F+EKERDAHYLRGLLPPAV +QELQ KR+M+NLR YEVPLHRY+A+MDLQERNERLFYK+
Sbjct: 61 FTEKERDAHYLRGLLPPAVFNQELQEKRLMYNLRQYEVPLHRYMALMDLQERNERLFYKV 120
Query: 125 LIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIV 184
LIDNVEELLP+VYTPTVGEACQKYGS++RRPQGL+ISLKEKGKILEVLKNWPE+NIQVIV
Sbjct: 121 LIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKNIQVIV 180
Query: 185 VTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYI 244
VTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPS+CLPITIDVGTNNE+LLNDEFYI
Sbjct: 181 VTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLPITIDVGTNNEKLLNDEFYI 240
Query: 245 GLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDD 304
GLRQKRA G+EY ELLDEFM AVKKNYGEKVLIQFEDFANHNAF+LL++YSSSHLVFNDD
Sbjct: 241 GLRQKRATGKEYAELLDEFMCAVKKNYGEKVLIQFEDFANHNAFDLLAKYSSSHLVFNDD 300
Query: 305 IQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETR 364
IQGTASVV+AGLLA+LKL+GG LADHTFLFLGAGEAGTGIAELIALEISKQT AP EETR
Sbjct: 301 IQGTASVVLAGLLASLKLVGGNLADHTFLFLGAGEAGTGIAELIALEISKQTKAPVEETR 360
Query: 365 KKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFT 424
KKIWLVDSKGLIV SR SLQHFKKPWAH+HE +K+L AV+AIKPTVLIG+SGVGKTFT
Sbjct: 361 KKIWLVDSKGLIVSSRLGSLQHFKKPWAHEHEPVKELVDAVKAIKPTVLIGSSGVGKTFT 420
Query: 425 KEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFV 484
KEVVE MAS N+KPLILALSNPTSQSECTAEEAYTWS+G+AIFASGSPFDPVEY+GKVFV
Sbjct: 421 KEVVETMASLNKKPLILALSNPTSQSECTAEEAYTWSKGQAIFASGSPFDPVEYEGKVFV 480
Query: 485 PGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRK 544
PGQANNAYIFPGFGLGLIMSG IRV D+MLLAASEALAAQVSQENYDKGLIYPPFTNIRK
Sbjct: 481 PGQANNAYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSQENYDKGLIYPPFTNIRK 540
Query: 545 ISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
ISA+IAA VAAKAYELGLAS LP+PKDLVK+AESCMYSP YR+Y
Sbjct: 541 ISAHIAANVAAKAYELGLASNLPQPKDLVKYAESCMYSPGYRNY 584
BLAST of Cucsa.161140 vs. NCBI nr
Match:
gi|1009135122|ref|XP_015884816.1| (PREDICTED: NADP-dependent malic enzyme-like [Ziziphus jujuba])
HSP 1 Score: 1010.7 bits (2612), Expect = 1.0e-291
Identity = 501/594 (84.34%), Postives = 552/594 (92.93%), Query Frame = 1
Query: 1 MESSMKKTMSNGSVHMDMEEIDNKF------EEAYGEHNATEDQLITPWTVSVASGYSLL 60
MES++K+ M +GS ++ KF E+ YGE ATEDQL+TPWTVS+ASGY+LL
Sbjct: 26 MESTLKE-MRSGSHD---NSVNGKFGFGGGVEDVYGEDRATEDQLVTPWTVSIASGYTLL 85
Query: 61 RDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQ 120
RDP +NKGLAF+EKERDAHYLRGLLPPA++ Q+LQ +++MH+LR Y+VPLH+YIAMMDLQ
Sbjct: 86 RDPHHNKGLAFTEKERDAHYLRGLLPPAIVSQDLQERKLMHSLRQYDVPLHKYIAMMDLQ 145
Query: 121 ERNERLFYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKN 180
ERNERLFYKLLIDNVEELLP+VYTPTVGEACQKYGS+YRRPQGL+ISLKEKGKILEVLKN
Sbjct: 146 ERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLYISLKEKGKILEVLKN 205
Query: 181 WPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNN 240
WPE++IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPSACLP+TIDVGTNN
Sbjct: 206 WPEKSIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGLRPSACLPVTIDVGTNN 265
Query: 241 EQLLNDEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRY 300
EQLLNDEFYIGL+Q+RARGQEY ELLDEFM AVK+NYGEKVL+QFEDFANHNAFELLSRY
Sbjct: 266 EQLLNDEFYIGLKQRRARGQEYTELLDEFMNAVKQNYGEKVLVQFEDFANHNAFELLSRY 325
Query: 301 SSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISK 360
SSSHLVFNDDIQGTASVV+AGL++ALKL+GG+LADHTFLFLGAGEAGTGIAELIALEISK
Sbjct: 326 SSSHLVFNDDIQGTASVVLAGLISALKLVGGSLADHTFLFLGAGEAGTGIAELIALEISK 385
Query: 361 QTGAPTEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLI 420
Q +P EE RKKIWLVDSKGLIV+SR SLQHFKKPWAH+HE +K+L AV+AIKPTVLI
Sbjct: 386 QIKSPVEEARKKIWLVDSKGLIVKSRLGSLQHFKKPWAHEHEPVKELVDAVKAIKPTVLI 445
Query: 421 GTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFD 480
G+SGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAY WS+GRAIFASGSPFD
Sbjct: 446 GSSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYKWSKGRAIFASGSPFD 505
Query: 481 PVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGL 540
PV Y+GKVFVPGQANNAYIFPGFGLGLI+SG IRVHDDMLLAASEALAAQV QENYDKGL
Sbjct: 506 PVNYEGKVFVPGQANNAYIFPGFGLGLIISGAIRVHDDMLLAASEALAAQVKQENYDKGL 565
Query: 541 IYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
IYPPF+NIRKISA+IAA VAAK YELGLAS LPRPK+LVK+AESCMYSP YRSY
Sbjct: 566 IYPPFSNIRKISAHIAANVAAKVYELGLASHLPRPKNLVKYAESCMYSPVYRSY 615
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
MAOX_PHAVU | 7.2e-290 | 84.67 | NADP-dependent malic enzyme OS=Phaseolus vulgaris GN=ME1 PE=2 SV=1 | [more] |
MAOC_FLAPR | 6.8e-280 | 82.59 | NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei GN=MODA PE=2 SV=... | [more] |
MAOX_VITVI | 8.3e-278 | 79.86 | NADP-dependent malic enzyme OS=Vitis vinifera PE=2 SV=1 | [more] |
MAOP3_ARATH | 1.6e-276 | 82.56 | NADP-dependent malic enzyme 3 OS=Arabidopsis thaliana GN=NADP-ME3 PE=1 SV=1 | [more] |
MAOX_POPTR | 1.0e-275 | 78.17 | NADP-dependent malic enzyme OS=Populus trichocarpa PE=2 SV=3 | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0LLH9_CUCSA | 0.0e+00 | 99.66 | Malic enzyme OS=Cucumis sativus GN=Csa_2G373430 PE=3 SV=1 | [more] |
X2C4W9_9FABA | 7.0e-292 | 85.62 | Malic enzyme OS=Stylosanthes guianensis PE=2 SV=1 | [more] |
V7AUA6_PHAVU | 5.9e-291 | 85.52 | Malic enzyme OS=Phaseolus vulgaris GN=PHAVU_009G111500g PE=3 SV=1 | [more] |
V7AXA3_PHAVU | 5.9e-291 | 85.52 | Malic enzyme OS=Phaseolus vulgaris GN=PHAVU_009G111500g PE=3 SV=1 | [more] |
A0A072VD34_MEDTR | 7.8e-291 | 87.74 | Malic enzyme OS=Medicago truncatula GN=MTR_3g110330 PE=3 SV=1 | [more] |