Cucsa.161140 (gene) Cucumber (Gy14) v1

NameCucsa.161140
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionMalic enzyme
Locationscaffold01144 : 1105046 .. 1109856 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTCACTATAAAACACGAATCTTGCTGCTTTCATTTCCACCAAATTCCTTCTGTATTTCCATTCTCTCTGTGTTGCCTTTGTTTTCGCTTTAACTTTCTGCCATTCGCAGAGAAACTTGGTGTGTAGAGGAAGGAGATTTGAGATTGAAGCTTTGGGATCAATGGAGAGTTCAATGAAGAAGACAATGAGCAACGGTAGTGTTCATATGGACATGGAAGAAATTGACAATAAGTTTGAAGAAGCCTATGGAGAACACAATGCTACTGAAGATCAGCTCATTACTCCTTGGACAGTCTCTGTTGCTAGGTAAGGGATTTTAAGGAAGATATTTGCTACTAAAAACACGTTCTGTTTATTTATTATTATTATTTTTTTGTGGTTGTTTTTGCATTTGTTCTAACTTGAAAATTTTCTCATTTTTGTCTGTCTTTTGTTGTTTTAGTGGTTATTCTTTACTGCGTGATCCGATCTACAACAAAGGCCTCGCCTTTAGTGAAAAAGAAAGAGATGCTCATTACTTGCGTGGCTTGTTGCCACCAGCAGTTATTGATCAAGAGCTTCAGGTAAAGTTTAGATCTTTCTAGTTCTACCATTTTCCCTTTTATTTACTTTTTTGTTGGAATGTCTTCCTATCTTGTGTTTGGGATGGTCGAGTTAAAATTCAGAGGGGTACACTACCTAAGATGTCTCACGCTAGTGGCGTCTTGATTATGTTATATGATATTTTCTCTCTAATAATAAACTTAAGATACTGATAGCAAACTATGCAAATCTTTTTGTCCACACTCTCTTACTTTACATTTTCTGTTTGTCTTTGTTGATAGCTTAACACTTTTTCTTTTTTTATGGTATTGATTTGATGCTTGTTTACACATTTTTTTTTTTTTGTGGGAAGCAGGAAAGGAGGATGATGCATAATCTTCGCAACTATGAAGTTCCACTACACAGATATATTGCCATGATGGATCTTCAGGTTCGATAGATGTTTCACTTGTTTATTGTTAATTTTGCTATTTGATTCCTCTCTCTGCAGCTAATGAGTTAAAGATCATTAGATGAAAAATATGAGTGTCATTGATATAAGGAGCTGTGGGGTTTGTCTTTGGAATTGAATTTTTAGGCTATATTATTAAAACGTATCTCTAAACTATGCTTTTTGTTTAAAAATATTATTATTCTTTCAAAAGTCCTAAGATTACTTTGACCTTATATAAATGTTAAAATTATCTTGGAGTCGAAATTGTTTATGATTTCTTATAGAAGTTGGGACTTGGAACTGTGTGGCGATCCAAAATTTTCACTTTTTAGTCATATCATTAAACTGCACACAAACAAGACTCTAAATCTTATTACTTATGATATGTAAGATGTTAATTTATCAAAGCCTGTTAGACAACCCAATTAAAGAATGTACTTTGCCTCCTTATCTCAATTGATGTTTGTAGTTGTACCTTTGTTCTACCTGGCCATACCACAAATTTTCCTTCATGTCTACTATCATTTGATCTTGCAGGAGAGAAATGAAAGGTTGTTCTACAAGCTTCTCATTGATAATGTTGAAGAACTGCTTCCAATTGTGTACACTCCAACCGTAGGCGAAGCTTGCCAGAAGTATGGGAGCGTTTATAGACGACCTCAAGGCCTTTTCATCAGTTTGAAAGAGAAGTATGTCATTAAACTAGCATTTATAATGTATGATTAGTTGCATTCGTTCTTAATTGGTAATCAACAATTTACCTTTCCAGGGGCAAGATTCTTGAAGTTTTGAAGAACTGGCCAGAAAGGAACATTCAAGTTATTGTTGTTACCGACGGTGAGCGGATTCTAGGCCTTGGGGATCTTGGATGCCAGGTAATAAAACTTACTGCTTCCACTCAGCTGATAAACCTTCATTTTTTCTTCTATCCTTCAGTTTTTTGTTGCATATTCTCATTTTGTTAAACTGATATCTTTCAGGGCATGGGGATTCCTGTGGGGAAACTTTCTTTATATACAGCACTTGGAGGAATTCGACCCTCAGCTGTAAGCAATGCTTTAGTTTATATACTTTAGTTAATCTCTTCCTCCCTTTGTTTATCATCAGTAATCTGAATACAACAAAGTGTTGATATCTGGAATAGTCAGGCGAGGACTAAAGTTTATAGGTGTTTATTGTGTTCAGTGCTTGCCTATAACCATTGATGTTGGTACAAACAACGAGCAGCTTCTAAATGATGAGTTTTACATTGGCCTTCGGCAGAAACGGGCCAGAGGACAGGTGAAACAATCTTTTACTCTCTTTTTACCTCCTAACTTAATGATTTCAATCCATTCATACATGAATGTTTTAATCAATTTTTGTTTTTTCCTTCATTCAGGAATACATGGAACTTTTAGACGAGTTCATGTATGCAGTAAAGAAGAACTACGGAGAGAAAGTCCTTATACAGGTTATTTGACAATTTTCAACTTACAACAGTAGGACAGAATTTTGCTTGGAGTTATGTACAAGTGATCCAATGTGTCGCTAACCATTTCTTACTTATTAAACAGTTTGAAGATTTTGCAAACCACAATGCATTCGAGTTGCTTTCAAGATATAGCTCGTCACACCTTGTGTTCAATGACGACATTCAAGTACGAAACAAACACGTGTTTCAGTTCACTCCTAATTTTTATATGTTTCAAGAGTATCTCTAACATGGTTTCAATCATTACGATATTTAGGGTACAGCATCTGTTGTTGTAGCAGGACTACTTGCAGCTCTTAAACTTATTGGAGGGACATTAGCTGACCACACTTTCTTATTCCTTGGAGCTGGAGAGGTGAGTTTCAAAATGAGTTATATTTGAAGTGTTTTGAACTGTTTTCGTCAACTTTGGCTTGTATTTCCTTGACGAATCATCTGCTTAAGTTTCATGTCTTGTCCCGTAATTTTTCATTCACTATCTTTGGTATGGTTCTTATTTCTGTCATTTGAGTGTTCTAGGCTGGAACTGGTATAGCTGAGCTTATTGCTTTGGAGATTTCAAAACAGGTACGAACCTCTTTCTACTTCTCTTCTCTTTTTTATTCTGAATCAATCAAATGCATTTATGGCTAATGATTTTTACTTGTTTTTGGCAGACTGGAGCTCCAACTGAAGAGACTCGCAAGAAGATTTGGCTCGTGGACTCGAAGGTTGTTTGGACTGACTCATTTGAATATTGTGCCTTTACACTCAAATGATCTTTCCTGTTCTAAATAAAATTATTACCATCTATTACAGGGGCTGATCGTTCAATCCCGCTTCGAATCCCTTCAACATTTTAAGAAGCCTTGGGCTCATGACCATGAATCTATCAAGGATCTTTACGGTGCCGTCCAGGTTAGTAATCTACATTGATCAGTCTTGCGTTATGTAGTCATTCTTCATAGTATATGAGACCATTTTTTAACTCAATCTTAAAACTTTATATTATTAGGCTATCAAACCAACAGTGTTGATAGGAACATCTGGTGTTGGGAAGACATTTACAAAGGAAGTTGTGGAGGCCATGGCATCCTTCAATGAGGTTCTTTCTTGTTCCTTCTAACTTTGTCATTCTTCAGTTCTAAGACAAGGAACTGTTTAAGATTAACTCAAGTGTCGATTACTATGCAGAAACCACTTATCCTTGCTCTCTCGAACCCTACGTCACAATCGGAGTGTACAGCTGAAGAAGCTTATACATGGAGCCAGGTACAATCCAAGTCTTTCAAAACAGCAGGCATAATTTTTTTTTTGTTTTGTTTGTGCTGACCACAATATAACAATAATTCACCAAAAAACGTGCAGGGACGAGCAATCTTTGCTAGCGGAAGTCCATTTGACCCTGTTGAATATGATGGAAAAGTTTTTGTGCCTGGCCAGGTAGCTTTCATGCTTCAGAAACCATTCTTTTAGTAACCATTCAATTTTAACAGCATGGTTTGATCTTATAAGCTTTGCCTTTTCTCTTGGTAATGTCATTTAGGCAAACAATGCTTACATATTCCCTGGATTTGGCTTGGGTCTGATCATGTCGGGTACAATTCGTGTACATGACGACATGCTCTTGGCAGCCTGTAAGTGAAATTTTGGTTAATTAACCAAACTAATAATGCTTGTTTTGTGCCAATCTTTATTCTCTCTTCCTCCTTCCAATGCTTGTTGAGCTAGTCTCCGAGTTGGAGCTTGATTTAACGGTTTTTTTCCCCTCATTGCAGCGGAGGCTCTGGCTGCCCAAGTGAGTCAGGAAAACTATGATAAGGGCTTGATTTACCCTCCTTTTACCAACATCAGAAAGATATCTGCTAACATTGCTGCCAAAGTTGCTGCTAAGGCTTATGAACTTGGTTAGCATCTCTCTCATTTTCTTCACATTGTGATTTAATTCATTCATAAACAAACCAATGACAGAAAAAGTTAACTAACAACATGGCATTACATTGCAGGTCTGGCTTCTCGTCTTCCTCGACCAAAGGATCTCGTAAAATTTGCCGAGAGCTGCATGTACAGCCCCCGCTATCGTAGCTACCTTTAAGCGAATATTGTAGTTTTAGTCCTCTAAAATCTTCCATCATTTTTTGTTTTGTGTAATTTGTTGTTTTTAGTCCTGGCATTAGCATAGGACATGCCTTAAAAAAAAGCTTTGTTACTTTTCTTTTATCTTATTGTTGTGTTTCTGAAGTTCTTTATCTCTTATGTTACATTCATTTGTTATTTATAGATCGAGTAATGTATTAGGCATTTTCAACTAAATGAAATTATTTGTGAGAAAAAGTAATAAAGAATAAAGCAGCAATTGACTGCTTGATCTGATGAGACC

mRNA sequence

CATTCACTATAAAACACGAATCTTGCTGCTTTCATTTCCACCAAATTCCTTCTGTATTTCCATTCTCTCTGTGTTGCCTTTGTTTTCGCTTTAACTTTCTGCCATTCGCAGAGAAACTTGGTGTGTAGAGGAAGGAGATTTGAGATTGAAGCTTTGGGATCAATGGAGAGTTCAATGAAGAAGACAATGAGCAACGGTAGTGTTCATATGGACATGGAAGAAATTGACAATAAGTTTGAAGAAGCCTATGGAGAACACAATGCTACTGAAGATCAGCTCATTACTCCTTGGACAGTCTCTGTTGCTAGTGGTTATTCTTTACTGCGTGATCCGATCTACAACAAAGGCCTCGCCTTTAGTGAAAAAGAAAGAGATGCTCATTACTTGCGTGGCTTGTTGCCACCAGCAGTTATTGATCAAGAGCTTCAGGAAAGGAGGATGATGCATAATCTTCGCAACTATGAAGTTCCACTACACAGATATATTGCCATGATGGATCTTCAGGAGAGAAATGAAAGGTTGTTCTACAAGCTTCTCATTGATAATGTTGAAGAACTGCTTCCAATTGTGTACACTCCAACCGTAGGCGAAGCTTGCCAGAAGTATGGGAGCGTTTATAGACGACCTCAAGGCCTTTTCATCAGTTTGAAAGAGAAGGGCAAGATTCTTGAAGTTTTGAAGAACTGGCCAGAAAGGAACATTCAAGTTATTGTTGTTACCGACGGTGAGCGGATTCTAGGCCTTGGGGATCTTGGATGCCAGGGCATGGGGATTCCTGTGGGGAAACTTTCTTTATATACAGCACTTGGAGGAATTCGACCCTCAGCTTGCTTGCCTATAACCATTGATGTTGGTACAAACAACGAGCAGCTTCTAAATGATGAGTTTTACATTGGCCTTCGGCAGAAACGGGCCAGAGGACAGGAATACATGGAACTTTTAGACGAGTTCATGTATGCAGTAAAGAAGAACTACGGAGAGAAAGTCCTTATACAGTTTGAAGATTTTGCAAACCACAATGCATTCGAGTTGCTTTCAAGATATAGCTCGTCACACCTTGTGTTCAATGACGACATTCAAGGTACAGCATCTGTTGTTGTAGCAGGACTACTTGCAGCTCTTAAACTTATTGGAGGGACATTAGCTGACCACACTTTCTTATTCCTTGGAGCTGGAGAGGCTGGAACTGGTATAGCTGAGCTTATTGCTTTGGAGATTTCAAAACAGACTGGAGCTCCAACTGAAGAGACTCGCAAGAAGATTTGGCTCGTGGACTCGAAGGGGCTGATCGTTCAATCCCGCTTCGAATCCCTTCAACATTTTAAGAAGCCTTGGGCTCATGACCATGAATCTATCAAGGATCTTTACGGTGCCGTCCAGGCTATCAAACCAACAGTGTTGATAGGAACATCTGGTGTTGGGAAGACATTTACAAAGGAAGTTGTGGAGGCCATGGCATCCTTCAATGAGAAACCACTTATCCTTGCTCTCTCGAACCCTACGTCACAATCGGAGTGTACAGCTGAAGAAGCTTATACATGGAGCCAGGGACGAGCAATCTTTGCTAGCGGAAGTCCATTTGACCCTGTTGAATATGATGGAAAAGTTTTTGTGCCTGGCCAGGCAAACAATGCTTACATATTCCCTGGATTTGGCTTGGGTCTGATCATGTCGGGTACAATTCGTGTACATGACGACATGCTCTTGGCAGCCTCGGAGGCTCTGGCTGCCCAAGTGAGTCAGGAAAACTATGATAAGGGCTTGATTTACCCTCCTTTTACCAACATCAGAAAGATATCTGCTAACATTGCTGCCAAAGTTGCTGCTAAGGCTTATGAACTTGGTCTGGCTTCTCGTCTTCCTCGACCAAAGGATCTCGTAAAATTTGCCGAGAGCTGCATGTACAGCCCCCGCTATCGTAGCTACCTTTAAGCGAATATTGTAGTTTTAGTCCTCTAAAATCTTCCATCATTTTTTGTTTTGTGTAATTTGTTGTTTTTAGTCCTGGCATTAGCATAGGACATGCCTTAAAAAAAAGCTTTGTTACTTTTCTTTTATCTTATTGTTGTGTTTCTGAAGTTCTTTATCTCTTATGTTACATTCATTTGTTATTTATAGATCGAGTAATGTATTAGGCATTTTCAACTAAATGAAATTATTTGTGAGAAAAAGTAATAAAGAATAAAGCAGCAATTGACTGCTTGATCTGATGAGACC

Coding sequence (CDS)

ATGGAGAGTTCAATGAAGAAGACAATGAGCAACGGTAGTGTTCATATGGACATGGAAGAAATTGACAATAAGTTTGAAGAAGCCTATGGAGAACACAATGCTACTGAAGATCAGCTCATTACTCCTTGGACAGTCTCTGTTGCTAGTGGTTATTCTTTACTGCGTGATCCGATCTACAACAAAGGCCTCGCCTTTAGTGAAAAAGAAAGAGATGCTCATTACTTGCGTGGCTTGTTGCCACCAGCAGTTATTGATCAAGAGCTTCAGGAAAGGAGGATGATGCATAATCTTCGCAACTATGAAGTTCCACTACACAGATATATTGCCATGATGGATCTTCAGGAGAGAAATGAAAGGTTGTTCTACAAGCTTCTCATTGATAATGTTGAAGAACTGCTTCCAATTGTGTACACTCCAACCGTAGGCGAAGCTTGCCAGAAGTATGGGAGCGTTTATAGACGACCTCAAGGCCTTTTCATCAGTTTGAAAGAGAAGGGCAAGATTCTTGAAGTTTTGAAGAACTGGCCAGAAAGGAACATTCAAGTTATTGTTGTTACCGACGGTGAGCGGATTCTAGGCCTTGGGGATCTTGGATGCCAGGGCATGGGGATTCCTGTGGGGAAACTTTCTTTATATACAGCACTTGGAGGAATTCGACCCTCAGCTTGCTTGCCTATAACCATTGATGTTGGTACAAACAACGAGCAGCTTCTAAATGATGAGTTTTACATTGGCCTTCGGCAGAAACGGGCCAGAGGACAGGAATACATGGAACTTTTAGACGAGTTCATGTATGCAGTAAAGAAGAACTACGGAGAGAAAGTCCTTATACAGTTTGAAGATTTTGCAAACCACAATGCATTCGAGTTGCTTTCAAGATATAGCTCGTCACACCTTGTGTTCAATGACGACATTCAAGGTACAGCATCTGTTGTTGTAGCAGGACTACTTGCAGCTCTTAAACTTATTGGAGGGACATTAGCTGACCACACTTTCTTATTCCTTGGAGCTGGAGAGGCTGGAACTGGTATAGCTGAGCTTATTGCTTTGGAGATTTCAAAACAGACTGGAGCTCCAACTGAAGAGACTCGCAAGAAGATTTGGCTCGTGGACTCGAAGGGGCTGATCGTTCAATCCCGCTTCGAATCCCTTCAACATTTTAAGAAGCCTTGGGCTCATGACCATGAATCTATCAAGGATCTTTACGGTGCCGTCCAGGCTATCAAACCAACAGTGTTGATAGGAACATCTGGTGTTGGGAAGACATTTACAAAGGAAGTTGTGGAGGCCATGGCATCCTTCAATGAGAAACCACTTATCCTTGCTCTCTCGAACCCTACGTCACAATCGGAGTGTACAGCTGAAGAAGCTTATACATGGAGCCAGGGACGAGCAATCTTTGCTAGCGGAAGTCCATTTGACCCTGTTGAATATGATGGAAAAGTTTTTGTGCCTGGCCAGGCAAACAATGCTTACATATTCCCTGGATTTGGCTTGGGTCTGATCATGTCGGGTACAATTCGTGTACATGACGACATGCTCTTGGCAGCCTCGGAGGCTCTGGCTGCCCAAGTGAGTCAGGAAAACTATGATAAGGGCTTGATTTACCCTCCTTTTACCAACATCAGAAAGATATCTGCTAACATTGCTGCCAAAGTTGCTGCTAAGGCTTATGAACTTGGTCTGGCTTCTCGTCTTCCTCGACCAAAGGATCTCGTAAAATTTGCCGAGAGCTGCATGTACAGCCCCCGCTATCGTAGCTACCTTTAA

Protein sequence

MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL*
BLAST of Cucsa.161140 vs. Swiss-Prot
Match: MAOX_PHAVU (NADP-dependent malic enzyme OS=Phaseolus vulgaris GN=ME1 PE=2 SV=1)

HSP 1 Score: 997.3 bits (2577), Expect = 7.2e-290
Identity = 497/587 (84.67%), Postives = 537/587 (91.48%), Query Frame = 1

Query: 2   ESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNK 61
           E S+KK  SNG    D+          YGE +ATED LITPWT SVASG SLLRDP YNK
Sbjct: 12  EVSVKKDYSNGGGVRDL----------YGEDSATEDHLITPWTFSVASGCSLLRDPRYNK 71

Query: 62  GLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLF 121
           GLAF+E ERDAHYLRGLLPP+V +QELQ KR+MHNLR YEVPLHRY+A+MDLQERNERLF
Sbjct: 72  GLAFTEGERDAHYLRGLLPPSVFNQELQEKRLMHNLRQYEVPLHRYMALMDLQERNERLF 131

Query: 122 YKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQ 181
           YKLLIDNV ELLP+VYTPTVGEACQKYGS++RRPQGL+ISLKEKGKILEVLKNWPE++IQ
Sbjct: 132 YKLLIDNVAELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKSIQ 191

Query: 182 VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDE 241
           VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPS+CLP+TIDVGTNNE+LLNDE
Sbjct: 192 VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNEKLLNDE 251

Query: 242 FYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVF 301
           FYIGLRQ+RA GQEY   LDEFM AVK+NYGEKVL+QFEDFANHNAF+LL +YSSSHLVF
Sbjct: 252 FYIGLRQRRATGQEYATFLDEFMRAVKQNYGEKVLVQFEDFANHNAFDLLEKYSSSHLVF 311

Query: 302 NDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTE 361
           NDDIQGTASVV+AGLLA+LKL+GGTLADHTFLFLGAGEAGTGIAELIA+E+SKQT AP E
Sbjct: 312 NDDIQGTASVVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIAVEVSKQTKAPVE 371

Query: 362 ETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGK 421
           ETRKKIWLVDSKGLIV SR ESLQ FKKPWAH+HE +K L  AV+AIKPTVLIG+SG GK
Sbjct: 372 ETRKKIWLVDSKGLIVSSRLESLQQFKKPWAHEHEPVKGLLEAVKAIKPTVLIGSSGAGK 431

Query: 422 TFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGK 481
           TFTKEVVE MAS NEKPLILALSNPTSQSECTAEEAYTWS+GRAIFASGSPFDPVEY+GK
Sbjct: 432 TFTKEVVETMASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGK 491

Query: 482 VFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTN 541
           +FVPGQANNAYIFPGFGLGLIMSG IRV D+MLLAASEALAAQVS+ENYDKGLIYPPFTN
Sbjct: 492 LFVPGQANNAYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSEENYDKGLIYPPFTN 551

Query: 542 IRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
           IRKISANIAAKVAAKAY+LGLAS L RPKDLVK+AESCMYSP YRSY
Sbjct: 552 IRKISANIAAKVAAKAYDLGLASHLKRPKDLVKYAESCMYSPGYRSY 588

BLAST of Cucsa.161140 vs. Swiss-Prot
Match: MAOC_FLAPR (NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei GN=MODA PE=2 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 6.8e-280
Identity = 465/563 (82.59%), Postives = 528/563 (93.78%), Query Frame = 1

Query: 26  EEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVID 85
           E+ YGE +ATED  ITPW+VSVASGYSLLRDP +NKGLAF+EKERDAHYLRGLLPP V++
Sbjct: 84  EDVYGEDSATEDHFITPWSVSVASGYSLLRDPHHNKGLAFTEKERDAHYLRGLLPPVVVN 143

Query: 86  QELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEAC 145
            +LQVK+MMHN+R YEVPL RY AMMDLQERNERLFYKLLI+N+EELLPIVYTPTVGEAC
Sbjct: 144 HDLQVKKMMHNIRQYEVPLQRYQAMMDLQERNERLFYKLLIENIEELLPIVYTPTVGEAC 203

Query: 146 QKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIP 205
           QKYG++++ PQGL+ISLK+KGK+LE+LKNWP++ IQVIVVTDGERILGLGDLGCQGMGIP
Sbjct: 204 QKYGTIFKNPQGLYISLKDKGKVLEILKNWPQKKIQVIVVTDGERILGLGDLGCQGMGIP 263

Query: 206 VGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMY 265
           VGKLSLYTALGGIRPSACLPITIDVGTNNE++LNDEFYIGLRQ+RA G+EY EL++EFM 
Sbjct: 264 VGKLSLYTALGGIRPSACLPITIDVGTNNEKMLNDEFYIGLRQRRASGKEYAELMNEFMS 323

Query: 266 AVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGG 325
           AVK+NYGEKVLIQFEDFANHNAF+LL +Y ++HLVFNDDIQGTASVV+AGL++ALKL+GG
Sbjct: 324 AVKQNYGEKVLIQFEDFANHNAFDLLEKYRTTHLVFNDDIQGTASVVLAGLISALKLVGG 383

Query: 326 TLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQ 385
           +LADH FLFLGAGEAGTGIAELIALEISKQT AP EETRKKIWLVDSKGLIV+SR +SLQ
Sbjct: 384 SLADHKFLFLGAGEAGTGIAELIALEISKQTNAPLEETRKKIWLVDSKGLIVRSRLDSLQ 443

Query: 386 HFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSN 445
           HFKKPWAHDHE +     AV+AIKPTVLIG+SG G+TFTKEVVEAM+SFNEKP+ILALSN
Sbjct: 444 HFKKPWAHDHEPVNKFLDAVKAIKPTVLIGSSGAGQTFTKEVVEAMSSFNEKPIILALSN 503

Query: 446 PTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSG 505
           PTSQSECTAE+AYTWS+GR IFASGSPF PVEY+GKV+V GQ+NNAYIFPGFGLGLI+SG
Sbjct: 504 PTSQSECTAEQAYTWSEGRTIFASGSPFAPVEYNGKVYVSGQSNNAYIFPGFGLGLIISG 563

Query: 506 TIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASR 565
            IRVHD+MLLAASEALA QV+QE++D GLIYPPFTNIRKISA+IAAKVAAKAYELGLASR
Sbjct: 564 AIRVHDEMLLAASEALAEQVTQEHFDNGLIYPPFTNIRKISAHIAAKVAAKAYELGLASR 623

Query: 566 LPRPKDLVKFAESCMYSPRYRSY 589
           LP+P++LV +AESCMYSP+YR+Y
Sbjct: 624 LPQPENLVAYAESCMYSPKYRNY 646

BLAST of Cucsa.161140 vs. Swiss-Prot
Match: MAOX_VITVI (NADP-dependent malic enzyme OS=Vitis vinifera PE=2 SV=1)

HSP 1 Score: 957.2 bits (2473), Expect = 8.3e-278
Identity = 472/591 (79.86%), Postives = 536/591 (90.69%), Query Frame = 1

Query: 1   MESSMKKTMSNGSVHMDMEE---IDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDP 60
           MES++K      SV +D++    +    E+ YGE  ATEDQL+TPWTVSVASGYSLLRDP
Sbjct: 1   MESTLKDIRDGASV-LDLDPKATVGGGVEDLYGEDFATEDQLVTPWTVSVASGYSLLRDP 60

Query: 61  IYNKGLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERN 120
            +NKGLAF++KERDAHYL GLLPP V  QELQ +++M+++R Y+VPL +Y+AMMDLQERN
Sbjct: 61  RHNKGLAFNDKERDAHYLCGLLPPVVSTQELQERKLMNSIRQYQVPLQKYMAMMDLQERN 120

Query: 121 ERLFYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPE 180
           ERLFYKLLIDNVEELLP+VYTPTVGEACQKYGS++RRPQGL+ISLKEKGKILEVLKNWPE
Sbjct: 121 ERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPE 180

Query: 181 RNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQL 240
           R IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPSACLPITIDVGTNNE+L
Sbjct: 181 RRIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTNNEKL 240

Query: 241 LNDEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSS 300
           L +EFYIGL+Q+RA G+EY E L EFM  VK+NYGEKVLIQFEDFANHNAF+LL++Y ++
Sbjct: 241 LANEFYIGLKQRRATGKEYSEFLQEFMSPVKQNYGEKVLIQFEDFANHNAFDLLAKYGTT 300

Query: 301 HLVFNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTG 360
           HL FNDDIQGTASVV+AG+++AL+L+GGTLADH FLFLGAGEAGTGIAELIALE+SKQT 
Sbjct: 301 HLAFNDDIQGTASVVLAGIVSALRLLGGTLADHKFLFLGAGEAGTGIAELIALEMSKQTK 360

Query: 361 APTEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTS 420
            P EETRKKIWLVDSKGLIV SR +SLQ FKKPWAH+HE +KDL  AV+ IKPTVLIG+S
Sbjct: 361 CPIEETRKKIWLVDSKGLIVGSRKDSLQQFKKPWAHEHEPVKDLLDAVKVIKPTVLIGSS 420

Query: 421 GVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVE 480
           GVGK FTKEV+EAMAS NEKPLILALSNPTSQSECTAEEAYTW+QGRAIFASGSPFDPVE
Sbjct: 421 GVGKAFTKEVIEAMASCNEKPLILALSNPTSQSECTAEEAYTWTQGRAIFASGSPFDPVE 480

Query: 481 YDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYP 540
           Y+GK FVPGQANNAYIFPG G+GL++SG IRVHD+MLLAASEALA QV+QEN+DKGLIYP
Sbjct: 481 YNGKTFVPGQANNAYIFPGLGMGLVISGAIRVHDEMLLAASEALARQVTQENFDKGLIYP 540

Query: 541 PFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
           PF+NIRKISA+IAA VAAKAYELGLA+RLP+P++LVK+AESCMYSP YRSY
Sbjct: 541 PFSNIRKISAHIAANVAAKAYELGLATRLPQPENLVKYAESCMYSPVYRSY 590

BLAST of Cucsa.161140 vs. Swiss-Prot
Match: MAOP3_ARATH (NADP-dependent malic enzyme 3 OS=Arabidopsis thaliana GN=NADP-ME3 PE=1 SV=1)

HSP 1 Score: 953.0 bits (2462), Expect = 1.6e-276
Identity = 464/562 (82.56%), Postives = 515/562 (91.64%), Query Frame = 1

Query: 27  EAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQ 86
           + YGE +AT DQL+TPW  SVASGY+L+RDP YNKGLAF++KERDAHY+ GLLPP V+ Q
Sbjct: 26  DVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQ 85

Query: 87  ELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEACQ 146
           ++Q +++MHNLR Y VPL RY+A+MDLQERNERLFYKLLIDNVEELLP+VYTPTVGEACQ
Sbjct: 86  DVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 145

Query: 147 KYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPV 206
           KYGS+YRRPQGL+ISLKEKGKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPV
Sbjct: 146 KYGSIYRRPQGLYISLKEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPV 205

Query: 207 GKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYA 266
           GKLSLYTALGGIRPSACLPITIDVGTNNE+LLN+EFYIGL+QKRA G+EY E L EFM A
Sbjct: 206 GKLSLYTALGGIRPSACLPITIDVGTNNEKLLNNEFYIGLKQKRANGEEYAEFLQEFMCA 265

Query: 267 VKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGT 326
           VK+NYGEKVL+QFEDFANH+AFELLS+Y SSHLVFNDDIQGTASVV+AGL+AA K++G +
Sbjct: 266 VKQNYGEKVLVQFEDFANHHAFELLSKYCSSHLVFNDDIQGTASVVLAGLIAAQKVLGKS 325

Query: 327 LADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQH 386
           LADHTFLFLGAGEAGTGIAELIAL+ISK+TG P +ETRKKIWLVDSKGLIV  R ESLQH
Sbjct: 326 LADHTFLFLGAGEAGTGIAELIALKISKETGKPIDETRKKIWLVDSKGLIVSERKESLQH 385

Query: 387 FKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNP 446
           FK+PWAHDH+ +K+L  AV AIKPTVLIGTSGVGKTFTKEVVEAMA+ NEKPLILALSNP
Sbjct: 386 FKQPWAHDHKPVKELLAAVNAIKPTVLIGTSGVGKTFTKEVVEAMATLNEKPLILALSNP 445

Query: 447 TSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGT 506
           TSQ+ECTAEEAYTW++GRAIFASGSPFDPV+YDGK F PGQANN YIFPG GLGLIMSG 
Sbjct: 446 TSQAECTAEEAYTWTKGRAIFASGSPFDPVQYDGKKFTPGQANNCYIFPGLGLGLIMSGA 505

Query: 507 IRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRL 566
           IRV DDMLLAASEALA+QV++EN+  GLIYPPF NIRKISANIAA V AK YELGLAS L
Sbjct: 506 IRVRDDMLLAASEALASQVTEENFANGLIYPPFANIRKISANIAASVGAKTYELGLASNL 565

Query: 567 PRPKDLVKFAESCMYSPRYRSY 589
           PRPKDLVK AESCMYSP YR++
Sbjct: 566 PRPKDLVKMAESCMYSPVYRNF 587

BLAST of Cucsa.161140 vs. Swiss-Prot
Match: MAOX_POPTR (NADP-dependent malic enzyme OS=Populus trichocarpa PE=2 SV=3)

HSP 1 Score: 950.3 bits (2455), Expect = 1.0e-275
Identity = 462/591 (78.17%), Postives = 535/591 (90.52%), Query Frame = 1

Query: 1   MESSMKKTMSNGSVHMDMEE---IDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDP 60
           MES++K+ M +G+  +DM+    +    E+ YGE  ATEDQL+TPWT+SVASGY+LLRDP
Sbjct: 1   MESTLKE-MRDGASVLDMDPKSTVGGGVEDVYGEDRATEDQLVTPWTISVASGYTLLRDP 60

Query: 61  IYNKGLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERN 120
            +NKGLAF+EKERDAHYLRGLLPP  I Q+LQ K++M+ +R Y++PL +Y AMM+L+ERN
Sbjct: 61  HHNKGLAFTEKERDAHYLRGLLPPTTISQQLQEKKLMNTIRQYQLPLQKYTAMMELEERN 120

Query: 121 ERLFYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPE 180
           ERLFYKLLIDNVEELLP+VYTPTVGEACQKYGS+++RPQGL+ISLKEKGK+L+VLKNWP+
Sbjct: 121 ERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFKRPQGLYISLKEKGKVLDVLKNWPQ 180

Query: 181 RNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQL 240
           ++IQVIVVTDGERILGLGDLGCQG+GIPVGKLSLYTALGG+RPSACLP+TIDVGTNNEQL
Sbjct: 181 KSIQVIVVTDGERILGLGDLGCQGIGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEQL 240

Query: 241 LNDEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSS 300
           L DEFYIGLRQ+RA GQEY ELL EFM AVK+NYGEKVLIQFEDFANHNAF+LL++Y ++
Sbjct: 241 LKDEFYIGLRQRRATGQEYSELLHEFMTAVKQNYGEKVLIQFEDFANHNAFDLLAKYGTT 300

Query: 301 HLVFNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTG 360
           HLVFNDDIQGTA+VV+AGL++ALKL+GG+LADHTFLFLGAGEAGTGIAELIALE+S+++ 
Sbjct: 301 HLVFNDDIQGTAAVVLAGLISALKLLGGSLADHTFLFLGAGEAGTGIAELIALEMSRRSK 360

Query: 361 APTEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTS 420
            P EETRKKIWL DSKGLIV SR ESLQHFKKPWAH+HE +K L   V+AIKP VLIGTS
Sbjct: 361 TPLEETRKKIWLTDSKGLIVSSRKESLQHFKKPWAHEHEPVKGLLEVVKAIKPIVLIGTS 420

Query: 421 GVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVE 480
           GVGKTFTKEV+EAMASFNEKPLILALSNPTSQSECTA+EAYTW++G+AIFASGSPFDPVE
Sbjct: 421 GVGKTFTKEVIEAMASFNEKPLILALSNPTSQSECTAQEAYTWTKGKAIFASGSPFDPVE 480

Query: 481 YDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYP 540
           Y+GKVFVPGQ+NNAYIFPG GLGL++SG IRVHDDMLLAA+EALA Q+ +E   KGLIYP
Sbjct: 481 YEGKVFVPGQSNNAYIFPGLGLGLVISGAIRVHDDMLLAAAEALAGQIKEEYLAKGLIYP 540

Query: 541 PFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
           P +NIRKIS  IAA VAAKAYELGLA+RLPRP++LVK AESCMYSP YR Y
Sbjct: 541 PLSNIRKISVQIAANVAAKAYELGLATRLPRPENLVKHAESCMYSPAYRYY 590

BLAST of Cucsa.161140 vs. TrEMBL
Match: A0A0A0LLH9_CUCSA (Malic enzyme OS=Cucumis sativus GN=Csa_2G373430 PE=3 SV=1)

HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 587/589 (99.66%), Postives = 588/589 (99.83%), Query Frame = 1

Query: 1   MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
           MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 1   MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60

Query: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
           KGLAFSEKERDAHYLRGLLPPAVIDQELQ +RMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120

Query: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
           FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180

Query: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
           QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240

Query: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
           EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV
Sbjct: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300

Query: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
           FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT
Sbjct: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360

Query: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
           EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG
Sbjct: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420

Query: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
           KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG
Sbjct: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480

Query: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
           KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT
Sbjct: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540

Query: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 590
           NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 589

BLAST of Cucsa.161140 vs. TrEMBL
Match: X2C4W9_9FABA (Malic enzyme OS=Stylosanthes guianensis PE=2 SV=1)

HSP 1 Score: 1010.7 bits (2612), Expect = 7.0e-292
Identity = 500/584 (85.62%), Postives = 541/584 (92.64%), Query Frame = 1

Query: 5   MKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLA 64
           M  T+ NG V      +D    + YGE  ATEDQLITPW  SV+SG +LLRDP YNKGLA
Sbjct: 1   MSSTLVNGGVEEASATVDGGVRDFYGEERATEDQLITPWNFSVSSGSTLLRDPRYNKGLA 60

Query: 65  FSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKL 124
           F+EKERDAHYLRGLLPPAV +QELQ KR+M+NLR YEVPLHRY+A+MDLQERNERLFYK+
Sbjct: 61  FTEKERDAHYLRGLLPPAVFNQELQEKRLMYNLRQYEVPLHRYMALMDLQERNERLFYKV 120

Query: 125 LIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIV 184
           LIDNVEELLP+VYTPTVGEACQKYGS++RRPQGL+ISLKEKGKILEVLKNWPE+NIQVIV
Sbjct: 121 LIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKNIQVIV 180

Query: 185 VTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYI 244
           VTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPS+CLPITIDVGTNNE+LLNDEFYI
Sbjct: 181 VTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLPITIDVGTNNEKLLNDEFYI 240

Query: 245 GLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDD 304
           GLRQKRA G+EY ELLDEFM AVKKNYGEKVLIQFEDFANHNAF+LL++YSSSHLVFNDD
Sbjct: 241 GLRQKRATGKEYAELLDEFMCAVKKNYGEKVLIQFEDFANHNAFDLLAKYSSSHLVFNDD 300

Query: 305 IQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETR 364
           IQGTASVV+AGLLA+LKL+GG LADHTFLFLGAGEAGTGIAELIALEISKQT AP EETR
Sbjct: 301 IQGTASVVLAGLLASLKLVGGNLADHTFLFLGAGEAGTGIAELIALEISKQTKAPVEETR 360

Query: 365 KKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFT 424
           KKIWLVDSKGLIV SR  SLQHFKKPWAH+HE +K+L  AV+AIKPTVLIG+SGVGKTFT
Sbjct: 361 KKIWLVDSKGLIVSSRLGSLQHFKKPWAHEHEPVKELVDAVKAIKPTVLIGSSGVGKTFT 420

Query: 425 KEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFV 484
           KEVVE MAS N+KPLILALSNPTSQSECTAEEAYTWS+G+AIFASGSPFDPVEY+GKVFV
Sbjct: 421 KEVVETMASLNKKPLILALSNPTSQSECTAEEAYTWSKGQAIFASGSPFDPVEYEGKVFV 480

Query: 485 PGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRK 544
           PGQANNAYIFPGFGLGLIMSG IRV D+MLLAASEALAAQVSQENYDKGLIYPPFTNIRK
Sbjct: 481 PGQANNAYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSQENYDKGLIYPPFTNIRK 540

Query: 545 ISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
           ISA+IAA VAAKAYELGLAS LP+PKDLVK+AESCMYSP YR+Y
Sbjct: 541 ISAHIAANVAAKAYELGLASNLPQPKDLVKYAESCMYSPGYRNY 584

BLAST of Cucsa.161140 vs. TrEMBL
Match: V7AUA6_PHAVU (Malic enzyme OS=Phaseolus vulgaris GN=PHAVU_009G111500g PE=3 SV=1)

HSP 1 Score: 1007.7 bits (2604), Expect = 5.9e-291
Identity = 502/587 (85.52%), Postives = 541/587 (92.16%), Query Frame = 1

Query: 2   ESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNK 61
           E S+KK  SNG    D+          YGE +ATED LITPWT SVASG SLLRDP YNK
Sbjct: 38  EVSVKKDYSNGGGVRDL----------YGEDSATEDHLITPWTFSVASGCSLLRDPRYNK 97

Query: 62  GLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLF 121
           GLAF+E ERDAHYLRGLLPP+V +QELQ KR+MHNLR YEVPLHRY+A+MDLQERNERLF
Sbjct: 98  GLAFTEGERDAHYLRGLLPPSVFNQELQEKRLMHNLRQYEVPLHRYMALMDLQERNERLF 157

Query: 122 YKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQ 181
           YKLLIDNVEELLP+VYTPTVGEACQKYGS++RRPQGL+ISLKEKGKILEVLKNWPE++IQ
Sbjct: 158 YKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKSIQ 217

Query: 182 VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDE 241
           VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPS+CLP+TIDVGTNNE+LLNDE
Sbjct: 218 VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNEKLLNDE 277

Query: 242 FYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVF 301
           FYIGLRQ+RA GQEY ELLDEFM AVK+NYGEKVL+QFEDFANHNAF+LL +YSSSHLVF
Sbjct: 278 FYIGLRQRRATGQEYAELLDEFMRAVKQNYGEKVLVQFEDFANHNAFDLLEKYSSSHLVF 337

Query: 302 NDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTE 361
           NDDIQGTASVV+AGLLA+LKL+GGTLADHTFLFLGAGEAGTGIAELIALE+SKQT AP E
Sbjct: 338 NDDIQGTASVVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTKAPVE 397

Query: 362 ETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGK 421
           ETRKKIWLVDSKGLIV SR ESLQ FKKPWAH+HE +K L  AV+AIKPTVLIG+SG GK
Sbjct: 398 ETRKKIWLVDSKGLIVSSRLESLQQFKKPWAHEHEPVKGLLEAVKAIKPTVLIGSSGAGK 457

Query: 422 TFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGK 481
           TFTKEVVE MAS NEKPLILALSNPTSQSECTAEEAYTWS+GRAIFASGSPFDPVEY+GK
Sbjct: 458 TFTKEVVETMASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGK 517

Query: 482 VFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTN 541
           +FVPGQANNAYIFPGFGLGLIMSG IRV D+MLLAASEALAAQVS+ENYDKGLIYPPFTN
Sbjct: 518 LFVPGQANNAYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSEENYDKGLIYPPFTN 577

Query: 542 IRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
           IRKISANIAAKVAAKAY+LGLAS LPRPKDLVK+AESCMYSP YRSY
Sbjct: 578 IRKISANIAAKVAAKAYDLGLASHLPRPKDLVKYAESCMYSPGYRSY 614

BLAST of Cucsa.161140 vs. TrEMBL
Match: V7AXA3_PHAVU (Malic enzyme OS=Phaseolus vulgaris GN=PHAVU_009G111500g PE=3 SV=1)

HSP 1 Score: 1007.7 bits (2604), Expect = 5.9e-291
Identity = 502/587 (85.52%), Postives = 541/587 (92.16%), Query Frame = 1

Query: 2   ESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNK 61
           E S+KK  SNG    D+          YGE +ATED LITPWT SVASG SLLRDP YNK
Sbjct: 12  EVSVKKDYSNGGGVRDL----------YGEDSATEDHLITPWTFSVASGCSLLRDPRYNK 71

Query: 62  GLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLF 121
           GLAF+E ERDAHYLRGLLPP+V +QELQ KR+MHNLR YEVPLHRY+A+MDLQERNERLF
Sbjct: 72  GLAFTEGERDAHYLRGLLPPSVFNQELQEKRLMHNLRQYEVPLHRYMALMDLQERNERLF 131

Query: 122 YKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQ 181
           YKLLIDNVEELLP+VYTPTVGEACQKYGS++RRPQGL+ISLKEKGKILEVLKNWPE++IQ
Sbjct: 132 YKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKSIQ 191

Query: 182 VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDE 241
           VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPS+CLP+TIDVGTNNE+LLNDE
Sbjct: 192 VIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNEKLLNDE 251

Query: 242 FYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVF 301
           FYIGLRQ+RA GQEY ELLDEFM AVK+NYGEKVL+QFEDFANHNAF+LL +YSSSHLVF
Sbjct: 252 FYIGLRQRRATGQEYAELLDEFMRAVKQNYGEKVLVQFEDFANHNAFDLLEKYSSSHLVF 311

Query: 302 NDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTE 361
           NDDIQGTASVV+AGLLA+LKL+GGTLADHTFLFLGAGEAGTGIAELIALE+SKQT AP E
Sbjct: 312 NDDIQGTASVVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTKAPVE 371

Query: 362 ETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGK 421
           ETRKKIWLVDSKGLIV SR ESLQ FKKPWAH+HE +K L  AV+AIKPTVLIG+SG GK
Sbjct: 372 ETRKKIWLVDSKGLIVSSRLESLQQFKKPWAHEHEPVKGLLEAVKAIKPTVLIGSSGAGK 431

Query: 422 TFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGK 481
           TFTKEVVE MAS NEKPLILALSNPTSQSECTAEEAYTWS+GRAIFASGSPFDPVEY+GK
Sbjct: 432 TFTKEVVETMASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGK 491

Query: 482 VFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTN 541
           +FVPGQANNAYIFPGFGLGLIMSG IRV D+MLLAASEALAAQVS+ENYDKGLIYPPFTN
Sbjct: 492 LFVPGQANNAYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSEENYDKGLIYPPFTN 551

Query: 542 IRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
           IRKISANIAAKVAAKAY+LGLAS LPRPKDLVK+AESCMYSP YRSY
Sbjct: 552 IRKISANIAAKVAAKAYDLGLASHLPRPKDLVKYAESCMYSPGYRSY 588

BLAST of Cucsa.161140 vs. TrEMBL
Match: A0A072VD34_MEDTR (Malic enzyme OS=Medicago truncatula GN=MTR_3g110330 PE=3 SV=1)

HSP 1 Score: 1007.3 bits (2603), Expect = 7.8e-291
Identity = 494/563 (87.74%), Postives = 535/563 (95.03%), Query Frame = 1

Query: 26  EEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVID 85
           ++ YGE +ATEDQLITPW  SVASG +LLRDP YNKGLAF+EKERDAHY+RGLLPPAV  
Sbjct: 15  KDLYGEDSATEDQLITPWNFSVASGCTLLRDPRYNKGLAFTEKERDAHYVRGLLPPAVFT 74

Query: 86  QELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEAC 145
           QELQ KR+MHNLR Y+VPLH+YIA+MDLQERNERLFYKL+IDNVEELLP+VYTPTVGEAC
Sbjct: 75  QELQEKRLMHNLRQYDVPLHKYIALMDLQERNERLFYKLMIDNVEELLPVVYTPTVGEAC 134

Query: 146 QKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIP 205
           QKYGS+YRRPQGL+ISLKEKGKILEVLKNWPE+ IQVIVVTDGERILGLGDLGCQGMGIP
Sbjct: 135 QKYGSIYRRPQGLYISLKEKGKILEVLKNWPEKTIQVIVVTDGERILGLGDLGCQGMGIP 194

Query: 206 VGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMY 265
           VGKLSLYTALGG+RPS+CLPITIDVGTNNE+LLNDEFYIGLRQKRA G+EY ELL+EFM+
Sbjct: 195 VGKLSLYTALGGVRPSSCLPITIDVGTNNEKLLNDEFYIGLRQKRATGKEYAELLEEFMH 254

Query: 266 AVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGG 325
           AVK+NYGEKVL+QFEDFANHNAF+LL +YSSSHLVFNDDIQGTASVV+AGLLA+LKL+GG
Sbjct: 255 AVKQNYGEKVLVQFEDFANHNAFDLLDKYSSSHLVFNDDIQGTASVVLAGLLASLKLVGG 314

Query: 326 TLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQ 385
           TLADHTFLFLGAGEAGTGIAELIALEISKQT AP EETRKKIWLVDSKGLIV SR +SLQ
Sbjct: 315 TLADHTFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKIWLVDSKGLIVSSRLQSLQ 374

Query: 386 HFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSN 445
           HFKKPWAH+HE +K+L  AV+AIKPTVLIG+SGVGKTFTKEVVEAMAS NEKPLILALSN
Sbjct: 375 HFKKPWAHEHEPVKELLNAVKAIKPTVLIGSSGVGKTFTKEVVEAMASLNEKPLILALSN 434

Query: 446 PTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSG 505
           PTSQSECTAEEAYTWS+G+AIFASGSPFDPVEY+GKVFVPGQ+NNAYIFPGFGLGLI+SG
Sbjct: 435 PTSQSECTAEEAYTWSKGKAIFASGSPFDPVEYEGKVFVPGQSNNAYIFPGFGLGLIISG 494

Query: 506 TIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASR 565
            IRV D+MLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAA VAAK YELGLAS 
Sbjct: 495 AIRVRDEMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAASVAAKTYELGLASH 554

Query: 566 LPRPKDLVKFAESCMYSPRYRSY 589
           LPRPKDLVK+AESCMYSP YRSY
Sbjct: 555 LPRPKDLVKYAESCMYSPGYRSY 577

BLAST of Cucsa.161140 vs. TAIR10
Match: AT5G25880.1 (AT5G25880.1 NADP-malic enzyme 3)

HSP 1 Score: 953.0 bits (2462), Expect = 8.8e-278
Identity = 464/562 (82.56%), Postives = 515/562 (91.64%), Query Frame = 1

Query: 27  EAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQ 86
           + YGE +AT DQL+TPW  SVASGY+L+RDP YNKGLAF++KERDAHY+ GLLPP V+ Q
Sbjct: 26  DVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQ 85

Query: 87  ELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEACQ 146
           ++Q +++MHNLR Y VPL RY+A+MDLQERNERLFYKLLIDNVEELLP+VYTPTVGEACQ
Sbjct: 86  DVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 145

Query: 147 KYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPV 206
           KYGS+YRRPQGL+ISLKEKGKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPV
Sbjct: 146 KYGSIYRRPQGLYISLKEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPV 205

Query: 207 GKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYA 266
           GKLSLYTALGGIRPSACLPITIDVGTNNE+LLN+EFYIGL+QKRA G+EY E L EFM A
Sbjct: 206 GKLSLYTALGGIRPSACLPITIDVGTNNEKLLNNEFYIGLKQKRANGEEYAEFLQEFMCA 265

Query: 267 VKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGT 326
           VK+NYGEKVL+QFEDFANH+AFELLS+Y SSHLVFNDDIQGTASVV+AGL+AA K++G +
Sbjct: 266 VKQNYGEKVLVQFEDFANHHAFELLSKYCSSHLVFNDDIQGTASVVLAGLIAAQKVLGKS 325

Query: 327 LADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQH 386
           LADHTFLFLGAGEAGTGIAELIAL+ISK+TG P +ETRKKIWLVDSKGLIV  R ESLQH
Sbjct: 326 LADHTFLFLGAGEAGTGIAELIALKISKETGKPIDETRKKIWLVDSKGLIVSERKESLQH 385

Query: 387 FKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNP 446
           FK+PWAHDH+ +K+L  AV AIKPTVLIGTSGVGKTFTKEVVEAMA+ NEKPLILALSNP
Sbjct: 386 FKQPWAHDHKPVKELLAAVNAIKPTVLIGTSGVGKTFTKEVVEAMATLNEKPLILALSNP 445

Query: 447 TSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGT 506
           TSQ+ECTAEEAYTW++GRAIFASGSPFDPV+YDGK F PGQANN YIFPG GLGLIMSG 
Sbjct: 446 TSQAECTAEEAYTWTKGRAIFASGSPFDPVQYDGKKFTPGQANNCYIFPGLGLGLIMSGA 505

Query: 507 IRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRL 566
           IRV DDMLLAASEALA+QV++EN+  GLIYPPF NIRKISANIAA V AK YELGLAS L
Sbjct: 506 IRVRDDMLLAASEALASQVTEENFANGLIYPPFANIRKISANIAASVGAKTYELGLASNL 565

Query: 567 PRPKDLVKFAESCMYSPRYRSY 589
           PRPKDLVK AESCMYSP YR++
Sbjct: 566 PRPKDLVKMAESCMYSPVYRNF 587

BLAST of Cucsa.161140 vs. TAIR10
Match: AT5G11670.1 (AT5G11670.1 NADP-malic enzyme 2)

HSP 1 Score: 943.7 bits (2438), Expect = 5.3e-275
Identity = 461/562 (82.03%), Postives = 515/562 (91.64%), Query Frame = 1

Query: 27  EAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQ 86
           + YGE +AT DQL+TPW  SVASGY+L+RDP YNKGLAF++KERDAHYL GLLPP ++ Q
Sbjct: 26  DVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYLTGLLPPVILSQ 85

Query: 87  ELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEACQ 146
           ++Q +++MHNLR Y VPL RY+A+MDLQERNERLFYKLLIDNVEELLP+VYTPTVGEACQ
Sbjct: 86  DVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 145

Query: 147 KYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPV 206
           KYGS++R+PQGL+ISL EKGKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPV
Sbjct: 146 KYGSIFRKPQGLYISLNEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPV 205

Query: 207 GKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYA 266
           GKLSLYTALGGIRPSACLPITIDVGTNNE+LLNDEFYIGL+Q+RA GQEY E L EFM A
Sbjct: 206 GKLSLYTALGGIRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGQEYAEFLHEFMCA 265

Query: 267 VKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGT 326
           VK+NYGEKVL+QFEDFANHNAF+LLS+YS SHLVFNDDIQGTASVV+AGL+AA K++G  
Sbjct: 266 VKQNYGEKVLVQFEDFANHNAFDLLSKYSDSHLVFNDDIQGTASVVLAGLIAAQKVLGKK 325

Query: 327 LADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQH 386
           LADHTFLFLGAGEAGTGIAELIAL+ISK+TGAP  ETRKKIWLVDSKGLIV SR ESLQH
Sbjct: 326 LADHTFLFLGAGEAGTGIAELIALKISKETGAPITETRKKIWLVDSKGLIVSSRKESLQH 385

Query: 387 FKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNP 446
           FK+PWAH+H+ +KDL GAV AIKPTVLIGTSGVG+TFTKEVVEAMA+ NEKPLILALSNP
Sbjct: 386 FKQPWAHEHKPVKDLIGAVNAIKPTVLIGTSGVGQTFTKEVVEAMATNNEKPLILALSNP 445

Query: 447 TSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGT 506
           TSQ+ECTAE+AYTW++GRAIF SGSPFDPV YDGK ++PGQANN YIFPG GLGLIMSG 
Sbjct: 446 TSQAECTAEQAYTWTKGRAIFGSGSPFDPVVYDGKTYLPGQANNCYIFPGLGLGLIMSGA 505

Query: 507 IRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRL 566
           IRV DDMLLAASEALAAQV++E+Y  GLIYPPF+NIR+ISANIAA VAAK Y+LGLAS L
Sbjct: 506 IRVRDDMLLAASEALAAQVTEEHYANGLIYPPFSNIREISANIAACVAAKTYDLGLASNL 565

Query: 567 PRPKDLVKFAESCMYSPRYRSY 589
           PR KDLVKFAES MYSP YR+Y
Sbjct: 566 PRAKDLVKFAESSMYSPVYRNY 587

BLAST of Cucsa.161140 vs. TAIR10
Match: AT1G79750.1 (AT1G79750.1 NADP-malic enzyme 4)

HSP 1 Score: 941.0 bits (2431), Expect = 3.5e-274
Identity = 457/563 (81.17%), Postives = 518/563 (92.01%), Query Frame = 1

Query: 26  EEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVID 85
           ++ YGE  ATED  ITPW++SVASGY+LLRDP +NKGLAFS +ERDAHYLRGLLPP VI 
Sbjct: 83  QDVYGEDAATEDMPITPWSLSVASGYTLLRDPHHNKGLAFSHRERDAHYLRGLLPPTVIS 142

Query: 86  QELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEAC 145
           Q+LQVK++MH LR Y+VPL +Y+AMMDLQE NERLFYKLLID+VEELLP++YTPTVGEAC
Sbjct: 143 QDLQVKKIMHTLRQYQVPLQKYMAMMDLQETNERLFYKLLIDHVEELLPVIYTPTVGEAC 202

Query: 146 QKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIP 205
           QKYGS++ RPQGLFISLKEKGKI EVL+NWPE+NIQVIVVTDGERILGLGDLGCQGMGIP
Sbjct: 203 QKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKNIQVIVVTDGERILGLGDLGCQGMGIP 262

Query: 206 VGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMY 265
           VGKLSLYTALGG+RPSACLP+TIDVGTNNE+LLNDEFYIGLRQ+RA G+EY EL+ EFM 
Sbjct: 263 VGKLSLYTALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQRRATGEEYSELMHEFMT 322

Query: 266 AVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGG 325
           AVK+NYGEKV+IQFEDFANHNAF+LL++Y ++HLVFNDDIQGTASVV+AGL+AAL+ +GG
Sbjct: 323 AVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTHLVFNDDIQGTASVVLAGLIAALRFVGG 382

Query: 326 TLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQ 385
           +L+DH FLFLGAGEAGTGIAELIALEISK++  P EE RK IWLVDSKGLIV SR ES+Q
Sbjct: 383 SLSDHRFLFLGAGEAGTGIAELIALEISKKSHIPLEEARKNIWLVDSKGLIVSSRKESIQ 442

Query: 386 HFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSN 445
           HFKKPWAHDHE I++L  AV+AIKPTVLIGTSGVG+TFT++VVE MA  NEKP+IL+LSN
Sbjct: 443 HFKKPWAHDHEPIRELVDAVKAIKPTVLIGTSGVGQTFTQDVVETMAKLNEKPIILSLSN 502

Query: 446 PTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSG 505
           PTSQSECTAEEAYTWSQGRAIFASGSPF PVEY+GK FVPGQANNAYIFPGFGLGLIMSG
Sbjct: 503 PTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKTFVPGQANNAYIFPGFGLGLIMSG 562

Query: 506 TIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASR 565
           TIRVHDDMLLAASEALA ++ +E+Y+KG+IYPPF NIRKISA IAAKVAAKAYELGLA+R
Sbjct: 563 TIRVHDDMLLAASEALAEELMEEHYEKGMIYPPFRNIRKISARIAAKVAAKAYELGLATR 622

Query: 566 LPRPKDLVKFAESCMYSPRYRSY 589
           LP+PK+L + AES MYSP YRSY
Sbjct: 623 LPQPKELEQCAESSMYSPSYRSY 645

BLAST of Cucsa.161140 vs. TAIR10
Match: AT2G19900.1 (AT2G19900.1 NADP-malic enzyme 1)

HSP 1 Score: 931.8 bits (2407), Expect = 2.1e-271
Identity = 454/568 (79.93%), Postives = 518/568 (91.20%), Query Frame = 1

Query: 21  IDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLP 80
           +D    + YGE +AT +  ITPW++SV+SGYSLLRDP YNKGLAF+EKERD HYLRGLLP
Sbjct: 13  VDGGVVDVYGEDSATIEHNITPWSLSVSSGYSLLRDPRYNKGLAFTEKERDTHYLRGLLP 72

Query: 81  PAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPT 140
           P V+DQ+LQ KR+++N+R Y+ PL +Y+A+ +LQERNERLFYKLLIDNVEELLPIVYTPT
Sbjct: 73  PVVLDQKLQEKRLLNNIRQYQFPLQKYMALTELQERNERLFYKLLIDNVEELLPIVYTPT 132

Query: 141 VGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQ 200
           VGEACQK+GS++RRPQGLFISLK+KGKIL+VLKNWPERNIQVIVVTDGERILGLGDLGCQ
Sbjct: 133 VGEACQKFGSIFRRPQGLFISLKDKGKILDVLKNWPERNIQVIVVTDGERILGLGDLGCQ 192

Query: 201 GMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELL 260
           GMGIPVGKL+LY+ALGG+RPSACLP+TIDVGTNNE+LLNDEFYIGLRQKRA GQEY ELL
Sbjct: 193 GMGIPVGKLALYSALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQKRATGQEYSELL 252

Query: 261 DEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAAL 320
           +EFM AVK+NYGEKVLIQFEDFANHNAFELL++YS +HLVFNDDIQGTASVV+AGL++A 
Sbjct: 253 NEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYSDTHLVFNDDIQGTASVVLAGLVSAQ 312

Query: 321 KLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSR 380
           KL    LA+HTFLFLGAGEAGTGIAELIAL +SKQ  A  EE+RKKIWLVDSKGLIV SR
Sbjct: 313 KLTNSPLAEHTFLFLGAGEAGTGIAELIALYMSKQMNASVEESRKKIWLVDSKGLIVNSR 372

Query: 381 FESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLI 440
            +SLQ FKKPWAH+HE +KDL GA++AIKPTVLIG+SGVG++FTKEV+EAM+S NE+PLI
Sbjct: 373 KDSLQDFKKPWAHEHEPVKDLLGAIKAIKPTVLIGSSGVGRSFTKEVIEAMSSINERPLI 432

Query: 441 LALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLG 500
           +ALSNPT+QSECTAEEAYTWS+GRAIFASGSPFDPVEY+GKVFV  QANNAYIFPGFGLG
Sbjct: 433 MALSNPTTQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKVFVSTQANNAYIFPGFGLG 492

Query: 501 LIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYEL 560
           L++SG IRVHDDMLLAA+EALA QVS+ENY+KG+IYP F++IRKISA IAA VA KAYEL
Sbjct: 493 LVISGAIRVHDDMLLAAAEALAGQVSKENYEKGMIYPSFSSIRKISAQIAANVATKAYEL 552

Query: 561 GLASRLPRPKDLVKFAESCMYSPRYRSY 589
           GLA RLPRPKD+VK AES MYSP YR Y
Sbjct: 553 GLAGRLPRPKDIVKCAESSMYSPTYRLY 580

BLAST of Cucsa.161140 vs. TAIR10
Match: AT2G13560.1 (AT2G13560.1 NAD-dependent malic enzyme 1)

HSP 1 Score: 438.3 bits (1126), Expect = 7.3e-123
Identity = 246/578 (42.56%), Postives = 357/578 (61.76%), Query Frame = 1

Query: 44  TVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVP 103
           T+    G  +L DP +NKG AF+  ER+   LRGLLPP V+D E Q+ R M +L+  E  
Sbjct: 40  TIVHKQGLDILHDPWFNKGTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQ 99

Query: 104 ----------LHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEACQKYGSVYR 163
                     L ++  +  L +RNE ++YK+LI+N+EE  PIVYTPTVG  CQ Y  ++R
Sbjct: 100 ARDGPSDPNALAKWRILNRLHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFR 159

Query: 164 RPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 223
           RP+G++ S +++G+++ ++ NWP   + +IVVTDG RILGLGDLG  G+GI VGKL LY 
Sbjct: 160 RPRGMYFSAEDRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVHGIGIAVGKLDLYV 219

Query: 224 ALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGE 283
           A  GI P   LP+ IDVGTNNE+L ND  Y+GL+Q+R    +Y++++DEFM AV   +  
Sbjct: 220 AAAGINPQRVLPVMIDVGTNNEKLRNDPMYLGLQQRRLEDDDYIDVIDEFMEAVYTRW-P 279

Query: 284 KVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLADH--- 343
            V++QFEDF +  AF+LL RY  ++ +FNDD+QGTA V +AGLL A++  G  + D    
Sbjct: 280 HVIVQFEDFQSKWAFKLLQRYRCTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKM 339

Query: 344 TFLFLGAGEAGTGIAELIALEISKQTG---APTEETRKKIWLVDSKGLIVQSRFESLQHF 403
             +  GAG AG G+       +++  G      +  + + W+VD++GLI + R E++   
Sbjct: 340 KIVVAGAGSAGIGVLNAARKTMARMLGNTETAFDSAQSQFWVVDAQGLITEGR-ENIDPE 399

Query: 404 KKPWAH-----DHESIKD---LYGAVQAIKPTVLIGTSGVGKTFTKEVVEAM-ASFNEKP 463
            +P+A      + + +K+   L   V+ +KP VL+G S VG  F+KEV+EAM  S + +P
Sbjct: 400 AQPFARKTKEMERQGLKEGATLVEVVREVKPDVLLGLSAVGGLFSKEVLEAMKGSTSTRP 459

Query: 464 LILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEY-DGKVFVPGQANNAYIFPGF 523
            I A+SNPT  +ECT ++A++      IFASGSPF  VE+ +G V    Q NN Y+FPG 
Sbjct: 460 AIFAMSNPTKNAECTPQDAFSILGENMIFASGSPFKNVEFGNGHVGHCNQGNNMYLFPGI 519

Query: 524 GLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKA 583
           GLG ++SG   V D ML AASE LAA +S+E   +G+IYPP + IR I+  IAA V  +A
Sbjct: 520 GLGTLLSGAPIVSDGMLQAASECLAAYMSEEEVLEGIIYPPISRIRDITKRIAAAVIKEA 579

Query: 584 YELGL--------ASRLPR--PKDLVKFAESCMYSPRY 586
            E  L        A  + +   + L+++ E+ M++P Y
Sbjct: 580 IEEDLVEGYREMDAREIQKLDEEGLMEYVENNMWNPEY 615

BLAST of Cucsa.161140 vs. NCBI nr
Match: gi|778672002|ref|XP_011649727.1| (PREDICTED: NADP-dependent malic enzyme [Cucumis sativus])

HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 587/589 (99.66%), Postives = 588/589 (99.83%), Query Frame = 1

Query: 1   MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
           MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 28  MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 87

Query: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
           KGLAFSEKERDAHYLRGLLPPAVIDQELQ +RMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 88  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 147

Query: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
           FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 148 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 207

Query: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
           QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 208 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 267

Query: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
           EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV
Sbjct: 268 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 327

Query: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
           FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT
Sbjct: 328 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 387

Query: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
           EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG
Sbjct: 388 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 447

Query: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
           KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG
Sbjct: 448 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 507

Query: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
           KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT
Sbjct: 508 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 567

Query: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 590
           NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 568 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 616

BLAST of Cucsa.161140 vs. NCBI nr
Match: gi|700207681|gb|KGN62800.1| (hypothetical protein Csa_2G373430 [Cucumis sativus])

HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 587/589 (99.66%), Postives = 588/589 (99.83%), Query Frame = 1

Query: 1   MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
           MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 1   MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60

Query: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
           KGLAFSEKERDAHYLRGLLPPAVIDQELQ +RMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120

Query: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
           FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180

Query: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
           QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240

Query: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
           EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV
Sbjct: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300

Query: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
           FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT
Sbjct: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360

Query: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
           EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG
Sbjct: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420

Query: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
           KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG
Sbjct: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480

Query: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
           KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT
Sbjct: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540

Query: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 590
           NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 589

BLAST of Cucsa.161140 vs. NCBI nr
Match: gi|659088473|ref|XP_008445000.1| (PREDICTED: NADP-dependent malic enzyme [Cucumis melo])

HSP 1 Score: 1156.4 bits (2990), Expect = 0.0e+00
Identity = 577/589 (97.96%), Postives = 586/589 (99.49%), Query Frame = 1

Query: 1   MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60
           MESSMKKTMSNGSVHM+MEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 1   MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60

Query: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERL 120
           KGLAFSEKER+AHYLRGLLPPA++DQELQ +RMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 61  KGLAFSEKERNAHYLRGLLPPALLDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120

Query: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180
           FYKLLIDNVEELLP+VYTPTVGEACQKYGS+YRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 121 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKGKILEVLKNWPERNI 180

Query: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240
           QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240

Query: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300
           EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV
Sbjct: 241 EFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLV 300

Query: 301 FNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPT 360
           FNDDIQGTASVVVAGLLAALKL+GGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP 
Sbjct: 301 FNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPI 360

Query: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420
           EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG
Sbjct: 361 EETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVG 420

Query: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480
           KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG
Sbjct: 421 KTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDG 480

Query: 481 KVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540
           K FVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT
Sbjct: 481 KAFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFT 540

Query: 541 NIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 590
           NIRKISANIAA+VAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 541 NIRKISANIAAEVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 589

BLAST of Cucsa.161140 vs. NCBI nr
Match: gi|468861715|gb|AGH32501.1| (malic enzyme [Stylosanthes guianensis])

HSP 1 Score: 1010.7 bits (2612), Expect = 1.0e-291
Identity = 500/584 (85.62%), Postives = 541/584 (92.64%), Query Frame = 1

Query: 5   MKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLA 64
           M  T+ NG V      +D    + YGE  ATEDQLITPW  SV+SG +LLRDP YNKGLA
Sbjct: 1   MSSTLVNGGVEEASATVDGGVRDFYGEERATEDQLITPWNFSVSSGSTLLRDPRYNKGLA 60

Query: 65  FSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKL 124
           F+EKERDAHYLRGLLPPAV +QELQ KR+M+NLR YEVPLHRY+A+MDLQERNERLFYK+
Sbjct: 61  FTEKERDAHYLRGLLPPAVFNQELQEKRLMYNLRQYEVPLHRYMALMDLQERNERLFYKV 120

Query: 125 LIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNIQVIV 184
           LIDNVEELLP+VYTPTVGEACQKYGS++RRPQGL+ISLKEKGKILEVLKNWPE+NIQVIV
Sbjct: 121 LIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPEKNIQVIV 180

Query: 185 VTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYI 244
           VTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPS+CLPITIDVGTNNE+LLNDEFYI
Sbjct: 181 VTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLPITIDVGTNNEKLLNDEFYI 240

Query: 245 GLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDD 304
           GLRQKRA G+EY ELLDEFM AVKKNYGEKVLIQFEDFANHNAF+LL++YSSSHLVFNDD
Sbjct: 241 GLRQKRATGKEYAELLDEFMCAVKKNYGEKVLIQFEDFANHNAFDLLAKYSSSHLVFNDD 300

Query: 305 IQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETR 364
           IQGTASVV+AGLLA+LKL+GG LADHTFLFLGAGEAGTGIAELIALEISKQT AP EETR
Sbjct: 301 IQGTASVVLAGLLASLKLVGGNLADHTFLFLGAGEAGTGIAELIALEISKQTKAPVEETR 360

Query: 365 KKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFT 424
           KKIWLVDSKGLIV SR  SLQHFKKPWAH+HE +K+L  AV+AIKPTVLIG+SGVGKTFT
Sbjct: 361 KKIWLVDSKGLIVSSRLGSLQHFKKPWAHEHEPVKELVDAVKAIKPTVLIGSSGVGKTFT 420

Query: 425 KEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFV 484
           KEVVE MAS N+KPLILALSNPTSQSECTAEEAYTWS+G+AIFASGSPFDPVEY+GKVFV
Sbjct: 421 KEVVETMASLNKKPLILALSNPTSQSECTAEEAYTWSKGQAIFASGSPFDPVEYEGKVFV 480

Query: 485 PGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRK 544
           PGQANNAYIFPGFGLGLIMSG IRV D+MLLAASEALAAQVSQENYDKGLIYPPFTNIRK
Sbjct: 481 PGQANNAYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSQENYDKGLIYPPFTNIRK 540

Query: 545 ISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
           ISA+IAA VAAKAYELGLAS LP+PKDLVK+AESCMYSP YR+Y
Sbjct: 541 ISAHIAANVAAKAYELGLASNLPQPKDLVKYAESCMYSPGYRNY 584

BLAST of Cucsa.161140 vs. NCBI nr
Match: gi|1009135122|ref|XP_015884816.1| (PREDICTED: NADP-dependent malic enzyme-like [Ziziphus jujuba])

HSP 1 Score: 1010.7 bits (2612), Expect = 1.0e-291
Identity = 501/594 (84.34%), Postives = 552/594 (92.93%), Query Frame = 1

Query: 1   MESSMKKTMSNGSVHMDMEEIDNKF------EEAYGEHNATEDQLITPWTVSVASGYSLL 60
           MES++K+ M +GS       ++ KF      E+ YGE  ATEDQL+TPWTVS+ASGY+LL
Sbjct: 26  MESTLKE-MRSGSHD---NSVNGKFGFGGGVEDVYGEDRATEDQLVTPWTVSIASGYTLL 85

Query: 61  RDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQELQVKRMMHNLRNYEVPLHRYIAMMDLQ 120
           RDP +NKGLAF+EKERDAHYLRGLLPPA++ Q+LQ +++MH+LR Y+VPLH+YIAMMDLQ
Sbjct: 86  RDPHHNKGLAFTEKERDAHYLRGLLPPAIVSQDLQERKLMHSLRQYDVPLHKYIAMMDLQ 145

Query: 121 ERNERLFYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKN 180
           ERNERLFYKLLIDNVEELLP+VYTPTVGEACQKYGS+YRRPQGL+ISLKEKGKILEVLKN
Sbjct: 146 ERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLYISLKEKGKILEVLKN 205

Query: 181 WPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNN 240
           WPE++IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPSACLP+TIDVGTNN
Sbjct: 206 WPEKSIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGLRPSACLPVTIDVGTNN 265

Query: 241 EQLLNDEFYIGLRQKRARGQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRY 300
           EQLLNDEFYIGL+Q+RARGQEY ELLDEFM AVK+NYGEKVL+QFEDFANHNAFELLSRY
Sbjct: 266 EQLLNDEFYIGLKQRRARGQEYTELLDEFMNAVKQNYGEKVLVQFEDFANHNAFELLSRY 325

Query: 301 SSSHLVFNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISK 360
           SSSHLVFNDDIQGTASVV+AGL++ALKL+GG+LADHTFLFLGAGEAGTGIAELIALEISK
Sbjct: 326 SSSHLVFNDDIQGTASVVLAGLISALKLVGGSLADHTFLFLGAGEAGTGIAELIALEISK 385

Query: 361 QTGAPTEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLI 420
           Q  +P EE RKKIWLVDSKGLIV+SR  SLQHFKKPWAH+HE +K+L  AV+AIKPTVLI
Sbjct: 386 QIKSPVEEARKKIWLVDSKGLIVKSRLGSLQHFKKPWAHEHEPVKELVDAVKAIKPTVLI 445

Query: 421 GTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFD 480
           G+SGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAY WS+GRAIFASGSPFD
Sbjct: 446 GSSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYKWSKGRAIFASGSPFD 505

Query: 481 PVEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGL 540
           PV Y+GKVFVPGQANNAYIFPGFGLGLI+SG IRVHDDMLLAASEALAAQV QENYDKGL
Sbjct: 506 PVNYEGKVFVPGQANNAYIFPGFGLGLIISGAIRVHDDMLLAASEALAAQVKQENYDKGL 565

Query: 541 IYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 589
           IYPPF+NIRKISA+IAA VAAK YELGLAS LPRPK+LVK+AESCMYSP YRSY
Sbjct: 566 IYPPFSNIRKISAHIAANVAAKVYELGLASHLPRPKNLVKYAESCMYSPVYRSY 615

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
MAOX_PHAVU7.2e-29084.67NADP-dependent malic enzyme OS=Phaseolus vulgaris GN=ME1 PE=2 SV=1[more]
MAOC_FLAPR6.8e-28082.59NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei GN=MODA PE=2 SV=... [more]
MAOX_VITVI8.3e-27879.86NADP-dependent malic enzyme OS=Vitis vinifera PE=2 SV=1[more]
MAOP3_ARATH1.6e-27682.56NADP-dependent malic enzyme 3 OS=Arabidopsis thaliana GN=NADP-ME3 PE=1 SV=1[more]
MAOX_POPTR1.0e-27578.17NADP-dependent malic enzyme OS=Populus trichocarpa PE=2 SV=3[more]
Match NameE-valueIdentityDescription
A0A0A0LLH9_CUCSA0.0e+0099.66Malic enzyme OS=Cucumis sativus GN=Csa_2G373430 PE=3 SV=1[more]
X2C4W9_9FABA7.0e-29285.62Malic enzyme OS=Stylosanthes guianensis PE=2 SV=1[more]
V7AUA6_PHAVU5.9e-29185.52Malic enzyme OS=Phaseolus vulgaris GN=PHAVU_009G111500g PE=3 SV=1[more]
V7AXA3_PHAVU5.9e-29185.52Malic enzyme OS=Phaseolus vulgaris GN=PHAVU_009G111500g PE=3 SV=1[more]
A0A072VD34_MEDTR7.8e-29187.74Malic enzyme OS=Medicago truncatula GN=MTR_3g110330 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G25880.18.8e-27882.56 NADP-malic enzyme 3[more]
AT5G11670.15.3e-27582.03 NADP-malic enzyme 2[more]
AT1G79750.13.5e-27481.17 NADP-malic enzyme 4[more]
AT2G19900.12.1e-27179.93 NADP-malic enzyme 1[more]
AT2G13560.17.3e-12342.56 NAD-dependent malic enzyme 1[more]
Match NameE-valueIdentityDescription
gi|778672002|ref|XP_011649727.1|0.0e+0099.66PREDICTED: NADP-dependent malic enzyme [Cucumis sativus][more]
gi|700207681|gb|KGN62800.1|0.0e+0099.66hypothetical protein Csa_2G373430 [Cucumis sativus][more]
gi|659088473|ref|XP_008445000.1|0.0e+0097.96PREDICTED: NADP-dependent malic enzyme [Cucumis melo][more]
gi|468861715|gb|AGH32501.1|1.0e-29185.62malic enzyme [Stylosanthes guianensis][more]
gi|1009135122|ref|XP_015884816.1|1.0e-29184.34PREDICTED: NADP-dependent malic enzyme-like [Ziziphus jujuba][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001891Malic_OxRdtase
IPR012301Malic_N_dom
IPR012302Malic_NAD-bd
IPR015884Malic_enzyme_CS
IPR016040NAD(P)-bd_dom
IPR001891Malic_OxRdtase
IPR012301Malic_N_dom
IPR012302Malic_NAD-bd
IPR015884Malic_enzyme_CS
IPR016040NAD(P)-bd_dom
Vocabulary: Molecular Function
TermDefinition
GO:0004470malic enzyme activity
GO:0004471malate dehydrogenase (decarboxylating) (NAD+) activity
GO:0051287NAD binding
GO:0004470malic enzyme activity
GO:0004471malate dehydrogenase (decarboxylating) (NAD+) activity
GO:0051287NAD binding
Vocabulary: Biological Process
TermDefinition
GO:0006108malate metabolic process
GO:0055114oxidation-reduction process
GO:0006108malate metabolic process
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015976 carbon utilization
biological_process GO:0006108 malate metabolic process
biological_process GO:0051260 protein homooligomerization
biological_process GO:0006090 pyruvate metabolic process
biological_process GO:0006099 tricarboxylic acid cycle
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004471 malate dehydrogenase (decarboxylating) (NAD+) activity
molecular_function GO:0004473 malate dehydrogenase (decarboxylating) (NADP+) activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051287 NAD binding
molecular_function GO:0004470 malic enzyme activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.161140.2Cucsa.161140.2mRNA
Cucsa.161140.1Cucsa.161140.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001891Malic oxidoreductasePRINTSPR00072MALOXRDTASEcoord: 120..144
score: 1.0E-85coord: 216..238
score: 1.0E-85coord: 435..451
score: 1.0E-85coord: 332..348
score: 1.0E-85coord: 276..294
score: 1.0E-85coord: 180..209
score: 1.0E-85coord: 301..317
score: 1.0
IPR001891Malic oxidoreductasePIRPIRSF000106MEcoord: 11..589
score: 1.3E
IPR012301Malic enzyme, N-terminal domainGENE3DG3DSA:3.40.50.10380coord: 49..302
score: 1.7E
IPR012301Malic enzyme, N-terminal domainPFAMPF00390maliccoord: 114..294
score: 5.0
IPR012301Malic enzyme, N-terminal domainSMARTSM01274malic_2coord: 114..295
score: 3.7E
IPR012302Malic enzyme, NAD-bindingPFAMPF03949Malic_Mcoord: 305..558
score: 3.0E
IPR012302Malic enzyme, NAD-bindingSMARTSM00919Malic_M_2coord: 305..558
score: 5.1E
IPR015884Malic enzyme, conserved sitePROSITEPS00331MALIC_ENZYMEScoord: 301..317
scor
IPR016040NAD(P)-binding domainGENE3DG3DSA:3.40.50.720coord: 303..588
score: 6.0E
IPR016040NAD(P)-binding domainunknownSSF51735NAD(P)-binding Rossmann-fold domainscoord: 305..588
score: 6.09
NoneNo IPR availableunknownCoilCoilcoord: 18..38
scor
NoneNo IPR availablePANTHERPTHR23406MALIC ENZYME-RELATEDcoord: 1..588
score:
NoneNo IPR availablePANTHERPTHR23406:SF31NADP-DEPENDENT MALIC ENZYME 2-RELATEDcoord: 1..588
score:
NoneNo IPR availableunknownSSF53223Aminoacid dehydrogenase-like, N-terminal domaincoord: 27..304
score: 4.53E

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.161140Cucumber (Chinese Long) v3cgycucB251
Cucsa.161140Watermelon (97103) v2cgywmbB274
Cucsa.161140Cucumber (Gy14) v1cgycgyB101
Cucsa.161140Cucurbita moschata (Rifu)cgycmoB0461
Cucsa.161140Melon (DHL92) v3.5.1cgymeB282
Cucsa.161140Watermelon (97103) v1cgywmB298
Cucsa.161140Bottle gourd (USVL1VR-Ls)cgylsiB263