Cucsa.148920 (gene) Cucumber (Gy14) v1

NameCucsa.148920
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionO-methyltransferase, putative
Locationscaffold01107 : 888148 .. 889827 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGGACACATCAACAAAGTGTGTTTTGAAAATTATTTCTCTCTTTCTGTTCTGAATATGAATATGGAGGGTGGAAAGATGTTGGCAATGGGAGGGGATGAGTTGTTAGAAGCTCAATCTCACGTATGGAATCACATCTTCAACTTCATAAACTCAATGTCTCTCAAATGTGCAATCCAACTTGGAATTCCAGACGCCATCCATAGCCATGGACCCAACCCTGTGCCTCTCTCTCTTCTTGTTTCATCTCTCCAACTTCATCCTAATAAAaCCCAATTCATATACCGTTTGATGCGTTTACTAACTCACTCTGGTTTCTTtGTTCAACAAGAAGAAGGATATATTCTTACCAATTCATCTCGCCTTCTCCTTAAAGACAATCCTTGTGCTGTATCTCCTTTCCTACTTTCCATGCTCCAGCCAGCCCTCACAGATCCATGGCAGTTCCTCTCAATCTGGTTCCAGACTGACGACCAAACGCCATTCGAGACAGCACACGGAGTGCCCTTCTGGAAGTACATGAGGAATAAGCCAAAAGAAGGGGAGGTTTTTAATGCAGGCATGGCGAGTGATGCGAGGTTGGTGATTAATGTGTTATTGGAAAAACATAGAAGTGTTTTTGAAGGAGTTGAGTCGTTGGTTGATGTTGGTGGTGGCACAGGAACTGTGGCCAAAGCCATTTCCCAAGCTTTTCCACAAATGGAATGCACTGTGTTGGATCTTCCCCAAGTAGTGGCTCATTTGAAGGGAGATCAACCAAACTTCAAATATGTTGAAGGAGATATGTTTACCCTTATTCCTCCGGCTGATACTATTCTGTTGAAGGTAGAACCTAATTTCTTTTGTTTTtCTTTTTATACACATATGGCTCCATTAGATAACATCTTTTTGTTTCCTTGCCACCGTCGTTTTTtGTTTTTGAAGAACTACAAATATAGTTACGTTTGAAATTAATTTTTTCTTTACAAAATATAATCAGAAATCGAAATAAAATTGTTGTGTGCAACTGAAGAAGACATTTATTTAAAATTCACCAATAAAGCATATCCACTAGTTTCTTTTAATCTTCCTAAACGATTCTTGCTGTATATGCTTATATTATAAAATCCAATAATCGTTTGTGGTGACTAAAATAGTGGATATTACACGACTGGAGCGATGAAGAATGTGTGGAGATTTTGAAGAAGTGCAAAGAAGCAATCACAGGCAGTGGTAGTAAGAAAGGGAAAGTGATGGTAATTGATTTGGTGTTATTTAATACGAAAAATGATGCAGATTCAATTGAGACACAACTATTGTACGACATGTTGATGATGATTGTACCTGGAGGAAAAGAAAGAGAGGAAAAGGAATGGGCTAAATTGATTAAAGAAGCTGGATTTAGGGCCTACAAGATCTTCCCTATTTTAGACTTAAGGTCTCTGATAGAGATTTATCCATGACATCACTTCCTTTTCTAACAACTTCATACAAGGGAGATGAATTTGATTACTGAATAAAGTATTTAGGTCAATCTTAATTAAGTCTGGATTGTTATGGTAGAAGTTATCTATTTTGGATTTAGTGAAACAATGTTGCCATTGAAGTTTGGAATAAAACAAATTTGCTTCCATATCATGTATTAGAAATTAGTATCTTTGATTTACAAATAAAATAAGATCATTTCATATTTACTATTTTA

mRNA sequence

TTGGACACATCAACAAAGTGTGTTTTGAAAATTATTTCTCTCTTTCTGTTCTGAATATGAATATGGAGGGTGGAAAGATGTTGGCAATGGGAGGGGATGAGTTGTTAGAAGCTCAATCTCACGTATGGAATCACATCTTCAACTTCATAAACTCAATGTCTCTCAAATGTGCAATCCAACTTGGAATTCCAGACGCCATCCATAGCCATGGACCCAACCCTGTGCCTCTCTCTCTTCTTGTTTCATCTCTCCAACTTCATCCTAATAAAACCCAATTCATATACCGTTTGATGCGTTTACTAACTCACTCTGGTTTCTTTGTTCAACAAGAAGAAGGATATATTCTTACCAATTCATCTCGCCTTCTCCTTAAAGACAATCCTTGTGCTGTATCTCCTTTCCTACTTTCCATGCTCCAGCCAGCCCTCACAGATCCATGGCAGTTCCTCTCAATCTGGTTCCAGACTGACGACCAAACGCCATTCGAGACAGCACACGGAGTGCCCTTCTGGAAGTACATGAGGAATAAGCCAAAAGAAGGGGAGGTTTTTAATGCAGGCATGGCGAGTGATGCGAGGTTGGTGATTAATGTGTTATTGGAAAAACATAGAAGTGTTTTTGAAGGAGTTGAGTCGTTGGTTGATGTTGGTGGTGGCACAGGAACTGTGGCCAAAGCCATTTCCCAAGCTTTTCCACAAATGGAATGCACTGTGTTGGATCTTCCCCAAGTAGTGGCTCATTTGAAGGGAGATCAACCAAACTTCAAATATGTTGAAGGAGATATGTTTACCCTTATTCCTCCGGCTGATACTATTCTGTTGAAGTGGATATTACACGACTGGAGCGATGAAGAATGTGTGGAGATTTTGAAGAAGTGCAAAGAAGCAATCACAGGCAGTGGTAGTAAGAAAGGGAAAGTGATGGTAATTGATTTGGTGTTATTTAATACGAAAAATGATGCAGATTCAATTGAGACACAACTATTGTACGACATGTTGATGATGATTGTACCTGGAGGAAAAGAAAGAGAGGAAAAGGAATGGGCTAAATTGATTAAAGAAGCTGGATTTAGGGCCTACAAGATCTTCCCTATTTTAGACTTAAGGTCTCTGATAGAGATTTATCCATGACATCACTTCCTTTTCTAACAACTTCATACAAGGGAGATGAATTTGATTACTGAATAAAGTATTTAGGTCAATCTTAATTAAGTCTGGATTGTTATGGTAGAAGTTATCTATTTTGGATTTAGTGAAACAATGTTGCCATTGAAGTTTGGAATAAAACAAATTTGCTTCCATATCATGTATTAGAAATTAGTATCTTTGATTTACAAATAAAATAAGATCATTTCATATTTACTATTTTA

Coding sequence (CDS)

ATGAATATGGAGGGTGGAAAGATGTTGGCAATGGGAGGGGATGAGTTGTTAGAAGCTCAATCTCACGTATGGAATCACATCTTCAACTTCATAAACTCAATGTCTCTCAAATGTGCAATCCAACTTGGAATTCCAGACGCCATCCATAGCCATGGACCCAACCCTGTGCCTCTCTCTCTTCTTGTTTCATCTCTCCAACTTCATCCTAATAAAaCCCAATTCATATACCGTTTGATGCGTTTACTAACTCACTCTGGTTTCTTtGTTCAACAAGAAGAAGGATATATTCTTACCAATTCATCTCGCCTTCTCCTTAAAGACAATCCTTGTGCTGTATCTCCTTTCCTACTTTCCATGCTCCAGCCAGCCCTCACAGATCCATGGCAGTTCCTCTCAATCTGGTTCCAGACTGACGACCAAACGCCATTCGAGACAGCACACGGAGTGCCCTTCTGGAAGTACATGAGGAATAAGCCAAAAGAAGGGGAGGTTTTTAATGCAGGCATGGCGAGTGATGCGAGGTTGGTGATTAATGTGTTATTGGAAAAACATAGAAGTGTTTTTGAAGGAGTTGAGTCGTTGGTTGATGTTGGTGGTGGCACAGGAACTGTGGCCAAAGCCATTTCCCAAGCTTTTCCACAAATGGAATGCACTGTGTTGGATCTTCCCCAAGTAGTGGCTCATTTGAAGGGAGATCAACCAAACTTCAAATATGTTGAAGGAGATATGTTTACCCTTATTCCTCCGGCTGATACTATTCTGTTGAAGTGGATATTACACGACTGGAGCGATGAAGAATGTGTGGAGATTTTGAAGAAGTGCAAAGAAGCAATCACAGGCAGTGGTAGTAAGAAAGGGAAAGTGATGGTAATTGATTTGGTGTTATTTAATACGAAAAATGATGCAGATTCAATTGAGACACAACTATTGTACGACATGTTGATGATGATTGTACCTGGAGGAAAAGAAAGAGAGGAAAAGGAATGGGCTAAATTGATTAAAGAAGCTGGATTTAGGGCCTACAAGATCTTCCCTATTTTAGACTTAAGGTCTCTGATAGAGATTTATCCATGA

Protein sequence

MNMEGGKMLAMGGDELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSLLVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSMLQPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVLLEKHRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVEGDMFTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKNDADSIETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP*
BLAST of Cucsa.148920 vs. Swiss-Prot
Match: ROMT_VITVI (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera GN=ROMT PE=1 SV=2)

HSP 1 Score: 415.2 bits (1066), Expect = 7.1e-115
Identity = 208/350 (59.43%), Postives = 255/350 (72.86%), Query Frame = 1

Query: 15  ELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSLLVSSLQLHPNKTQF 74
           ELL AQ+HVWNHIFNFI SMSLKCAIQLGIPD IH+HG  P+ L  LV+ L +HP ++Q 
Sbjct: 11  ELLHAQAHVWNHIFNFIKSMSLKCAIQLGIPDIIHNHG-KPMTLPELVAKLPVHPKRSQC 70

Query: 75  IYRLMRLLTHSGFFVQQ-------EEGYILTNSSRLLLKDNPCAVSPFLLSMLQPALTDP 134
           +YRLMR+L HSGF   Q       EEGY+LT++SRLLL D+  ++ P +L+ML P LT P
Sbjct: 71  VYRLMRILVHSGFLAAQRVQQGKEEEGYVLTDASRLLLMDDSLSIRPLVLAMLDPILTKP 130

Query: 135 WQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVLLEKHRSV 194
           W +LS WFQ DD TPF TA+   FW Y  ++P+    FN  MASDARL+ +VLL++ + V
Sbjct: 131 WHYLSAWFQNDDPTPFHTAYERSFWDYAGHEPQLNNSFNEAMASDARLLTSVLLKEGQGV 190

Query: 195 FEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVEGDMFTLI 254
           F G+ SLVDVGGGTG VAKAI+ AFP + CTVLDL  VVA L+G + N  Y  GDMF  I
Sbjct: 191 FAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAGLQGSK-NLNYFAGDMFEAI 250

Query: 255 PPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKNDADSIET 314
           PPAD ILLKWILHDWS+EECV+ILK+C+EAI  S    GKV++ID+++   + D  S ET
Sbjct: 251 PPADAILLKWILHDWSNEECVKILKRCREAIP-SKENGGKVIIIDMIMMKNQGDYKSTET 310

Query: 315 QLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 358
           QL +DM MMI   G+ER+E EW KL  +AGF  YKI PIL LRSLIE+YP
Sbjct: 311 QLFFDMTMMIFAPGRERDENEWEKLFLDAGFSHYKITPILGLRSLIEVYP 357

BLAST of Cucsa.148920 vs. Swiss-Prot
Match: Q8OMT_MENPI (8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita GN=OMT2 PE=1 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.4e-99
Identity = 183/357 (51.26%), Postives = 246/357 (68.91%), Query Frame = 1

Query: 15  ELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSLLVSSLQLHPNKTQF 74
           ELLEAQ+HVWNHI+++INSMSLKCAIQLGIPDAIH HG NP+ LS L  +L ++  K+  
Sbjct: 12  ELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHG-NPITLSQLADALNINKAKSHG 71

Query: 75  IYRLMRLLTHSGFF--------------VQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 134
           ++RLMR+L HSGFF               ++E+ Y LT +SRLLL+  P +V+PF L+M 
Sbjct: 72  LFRLMRILVHSGFFDKVKVKVKVEGEDEEEEEDAYSLTPASRLLLRSEPLSVAPFALAMS 131

Query: 135 QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVL 194
            P  T+ W  LS WF+ D    F+T +G+ F +Y     +   +FN  MA DA  V ++L
Sbjct: 132 DPVYTETWHHLSEWFRNDAVAAFDTKYGMTFPEYAVADDRLNVLFNEAMACDAGFVNSIL 191

Query: 195 LEKHRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVE 254
             + R +F+G+ES+VDVGGGTG  AK I+ AFP MECTVLDLP VV  LKG + N  +V 
Sbjct: 192 TTECREIFDGLESMVDVGGGTGATAKGIAAAFPGMECTVLDLPNVVGGLKGSE-NLSFVS 251

Query: 255 GDMFTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKN 314
           GDMF  IP AD I +K+ILHDW+DEECV+ILKKCKEAI+ S +   K++++++V+ + K 
Sbjct: 252 GDMFDFIPHADAIFMKFILHDWNDEECVKILKKCKEAISRSNNSCRKIILVEIVMEDEKE 311

Query: 315 DADSIETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 358
             ++ ET+L +DM M+ +  GKER EKEW KL  +AGF  YKI  +L LRS+IE++P
Sbjct: 312 THEATETKLFFDMQMLAIITGKERSEKEWGKLFFDAGFTNYKITRVLGLRSVIEVFP 366

BLAST of Cucsa.148920 vs. Swiss-Prot
Match: HTOMT_CATRO (Tabersonine 16-O-methyltransferase OS=Catharanthus roseus GN=16OMT PE=1 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 4.2e-91
Identity = 177/355 (49.86%), Postives = 240/355 (67.61%), Query Frame = 1

Query: 14  DELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSLLVSSL--QLHPNK 73
           +E   AQ+ +W+   +FI S SLKCA++LGIPD I +HG  P+ LS L ++L   +HP+K
Sbjct: 6   EEFRGAQAQIWSQSCSFITSASLKCAVKLGIPDTIDNHG-KPITLSELTNALVPPVHPSK 65

Query: 74  TQFIYRLMRLLTHSGFFVQQ------EEGYILTNSSRLLLKDNPCAVSPFLLSMLQPALT 133
             FIYRLMR+L  +GF  ++      E  Y LT SSR+LLK  P  +   +L+M  P   
Sbjct: 66  APFIYRLMRVLAKNGFCSEEQLDGETEPLYSLTPSSRILLKKEPLNLRGIVLTMADPVQL 125

Query: 134 DPWQFLSIWFQTDDQ--TPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVLLEK 193
             W+ LS W+Q +D   T FETAHG  FW Y     +  E FN  MASD++L+  +L+ +
Sbjct: 126 KAWESLSDWYQNEDDSSTAFETAHGKNFWGYSSEHMEHAEFFNEAMASDSQLISKLLIGE 185

Query: 194 HRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVEGDM 253
           ++ +FEG+ SLVD+GGGTGT+AKAI++ FPQ++CTV DLP VVA+L+  + N ++V GDM
Sbjct: 186 YKFLFEGLASLVDIGGGTGTIAKAIAKNFPQLKCTVFDLPHVVANLESKE-NVEFVAGDM 245

Query: 254 FTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKNDAD 313
           F  IP A+ I LKWILHDW+DE+CV+ILK CK+AI   G   GKV++ID+V+++ K D  
Sbjct: 246 FEKIPSANAIFLKWILHDWNDEDCVKILKSCKKAIPAKG---GKVIIIDMVMYSDKKDDH 305

Query: 314 SIETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDL-RSLIEIYP 358
            ++TQ   DM M++    KER EKEWA L KEAGF  YKI+P LD  RSLIE+YP
Sbjct: 306 LVKTQTSMDMAMLVNFAAKERCEKEWAFLFKEAGFSDYKIYPKLDFTRSLIEVYP 355

BLAST of Cucsa.148920 vs. Swiss-Prot
Match: MOMT_CATRO (Myricetin O-methyltransferase OS=Catharanthus roseus PE=1 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 7.9e-90
Identity = 172/352 (48.86%), Postives = 246/352 (69.89%), Query Frame = 1

Query: 9   LAMGGDELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSLLVSSLQLH 68
           + +   E+  AQ+H +  +F+F +  SLKCA+QLGIPDAIHSHG  P+ LS L +SL ++
Sbjct: 1   MELQSSEIRNAQAHFFTQVFSFTSMSSLKCAVQLGIPDAIHSHG-KPMALSDLTNSLPIN 60

Query: 69  PNKTQFIYRLMRLLTHSGFFVQQEEG-YILTNSSRLLLKDNPCAVSPFLLSMLQPALTDP 128
           P+K  +IYRLMR+L  +G+F ++E+  Y LT  +RLLLK++P      +L + Q A    
Sbjct: 61  PSKAPYIYRLMRILVAAGYFSEEEKNVYSLTPFTRLLLKNDPLNSISMVLGVNQIAELKA 120

Query: 129 WQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVLLEKHRSV 188
           W  +S WFQ +D T FETAHG  FW +   + K G+ F+  MA+D+ LV  +L+ +   +
Sbjct: 121 WNAMSEWFQNEDLTAFETAHGKNFWDFGA-EDKYGKNFDGVMAADSILVSKMLIPEFNYL 180

Query: 189 FEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVEGDMFTLI 248
           FEG++SLVDVGGGTGT+AKAI+++FP ++CTV DLP VVA+L+  + N ++V GDMF  I
Sbjct: 181 FEGLDSLVDVGGGTGTIAKAIAKSFPDLKCTVFDLPHVVANLESTE-NLEFVGGDMFEKI 240

Query: 249 PPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTK--NDADSI 308
           P A+ ILLKWILHDW DEECV++LK C++AI     K GKV++I+ VL ++K   + +++
Sbjct: 241 PSANAILLKWILHDWKDEECVKVLKMCRKAIP-EKEKGGKVILIETVLMDSKKHENEEAV 300

Query: 309 ETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 358
           + Q+  D+ MM+    KER E+EWA L +EAGF  YKIFP++D RS IE+YP
Sbjct: 301 KAQISSDIDMMVFFTAKERTEEEWATLFREAGFSGYKIFPMIDFRSPIEVYP 348

BLAST of Cucsa.148920 vs. Swiss-Prot
Match: CVMT1_OCIBA (Chavicol O-methyltransferase OS=Ocimum basilicum GN=CVOMT1 PE=1 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 7.9e-90
Identity = 175/356 (49.16%), Postives = 241/356 (67.70%), Query Frame = 1

Query: 9   LAMGGDELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSLLVSSLQLH 68
           +++  ++LL+AQ+HVWNH++ F NSMSLKCAIQLGIPD +H H  +P+ LS L+ ++ ++
Sbjct: 8   ISLSTEQLLQAQAHVWNHMYAFANSMSLKCAIQLGIPDILHKHD-HPMTLSQLLKAIPIN 67

Query: 69  PNKTQFIYRLMRLLTHSGFFVQ-----QEEGYILTNSSRLLLKDNPCAVSPFLLSMLQPA 128
             K+Q   RLMR L +S FF++     QE  Y LT +SRLLLK  P  V+P +  +L P 
Sbjct: 68  KEKSQSFQRLMRALVNSNFFIEENSNNQEVCYWLTPASRLLLKGAPLTVAPLVQVVLDPT 127

Query: 129 LTDPWQFLSIWFQTDDQ-TPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVLLE 188
            T+PW ++S WF+ ++  T FE A+G  FW+ + NKP  G  F+  M+ D+RLV +VL +
Sbjct: 128 FTNPWHYMSEWFKHENHATQFEAANGCTFWEKLANKPSMGRFFDEAMSCDSRLVAHVLTK 187

Query: 189 KHRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVEGD 248
            ++ V +G+ +LVDVGGG GT+AKAI +A P M+CTVLDLP VVA L+       Y+ GD
Sbjct: 188 DYKHVIDGIRTLVDVGGGNGTMAKAIVEAVPTMKCTVLDLPHVVAGLESTD-KLSYIGGD 247

Query: 249 MFTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKNDA 308
           MF  IP AD ILLK+I+HDW DEE ++ILK+CK+A+ G G   GKV++ID+V+    +D 
Sbjct: 248 MFQSIPSADAILLKFIIHDWDDEEGLKILKRCKDAV-GIG---GKVIIIDVVV-GVNHDV 307

Query: 309 DSI-ETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 358
           D + E QL +DM MM     KER   EW KLI  AGF +YK+ P   +RSLIE YP
Sbjct: 308 DEVLEDQLHFDMAMMSYFNAKERTMNEWEKLISAAGFTSYKLTPAFGVRSLIEAYP 356

BLAST of Cucsa.148920 vs. TrEMBL
Match: A0A0A0K5D5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G039790 PE=3 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 2.1e-206
Identity = 356/357 (99.72%), Postives = 357/357 (100.00%), Query Frame = 1

Query: 1   MNMEGGKMLAMGGDELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSL 60
           MNMEGGKMLAMGGDELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLS+
Sbjct: 1   MNMEGGKMLAMGGDELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSI 60

Query: 61  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 120
           LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML
Sbjct: 61  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 120

Query: 121 QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVL 180
           QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVL
Sbjct: 121 QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVL 180

Query: 181 LEKHRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVE 240
           LEKHRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVE
Sbjct: 181 LEKHRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVE 240

Query: 241 GDMFTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKN 300
           GDMFTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKN
Sbjct: 241 GDMFTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKN 300

Query: 301 DADSIETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 358
           DADSIETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP
Sbjct: 301 DADSIETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 357

BLAST of Cucsa.148920 vs. TrEMBL
Match: A0A0A0K1E9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G039280 PE=3 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 1.4e-181
Identity = 315/357 (88.24%), Postives = 333/357 (93.28%), Query Frame = 1

Query: 1   MNMEGGKMLAMGGDELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSL 60
           MNMEGG MLAMGGDELLEAQSH+WNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLS+
Sbjct: 26  MNMEGGNMLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSI 85

Query: 61  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 120
           LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML
Sbjct: 86  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 145

Query: 121 QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVL 180
           QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLV +VL
Sbjct: 146 QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVNSVL 205

Query: 181 LEKHRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVE 240
           L KH+SVFEGV+SLVDVGGGTGT+AKAISQAFPQMECTV DLPQVVAHL+GDQPN  YVE
Sbjct: 206 LGKHKSVFEGVKSLVDVGGGTGTMAKAISQAFPQMECTVFDLPQVVAHLRGDQPNLNYVE 265

Query: 241 GDMFTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKN 300
           GDMF  IPPAD +LLKWILHDWSDEECVEILK CK AI  +G+ KGKVMVID+VLF    
Sbjct: 266 GDMFKRIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGN-KGKVMVIDIVLFGNYK 325

Query: 301 DADSIETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 358
             DS+ETQLL+DMLMM + GGKEREEKEWAKLIKEAGF +YKIFPI+ +RSL+EIYP
Sbjct: 326 -KDSMETQLLFDMLMMTLVGGKEREEKEWAKLIKEAGFGSYKIFPIMGVRSLVEIYP 380

BLAST of Cucsa.148920 vs. TrEMBL
Match: W9R084_9ROSA (8-hydroxyquercetin 8-O-methyltransferase OS=Morus notabilis GN=L484_014739 PE=3 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 8.1e-126
Identity = 221/352 (62.78%), Postives = 273/352 (77.56%), Query Frame = 1

Query: 15  ELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSLLVSSLQLHPNKTQF 74
           ELL AQ+H+WN+IFNFINSMSLKCA+QLGIPD IH HG  P+ LS L+ +L +H NK  +
Sbjct: 24  ELLGAQAHIWNNIFNFINSMSLKCAVQLGIPDTIHKHG-KPMTLSKLIGALPVHQNKAPY 83

Query: 75  IYRLMRLLTHSGFFV---------QQEEGYILTNSSRLLLKDNPCAVSPFLLSMLQPALT 134
           +YRLMR+L HSGFF          Q+EEGY+LT++S+LL+ DNP +V+PFLL+ML P LT
Sbjct: 84  VYRLMRILVHSGFFSLQTIGESDHQEEEGYVLTDASKLLVGDNPLSVAPFLLAMLDPVLT 143

Query: 135 DPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVLLEKHR 194
            PW FLS WFQ DD T F+T +G+PFWKY   +PK    FN  MASDARLVI+V++E  +
Sbjct: 144 KPWDFLSTWFQNDDPTAFDTVNGLPFWKYGGQEPKMANFFNDAMASDARLVISVVIENCK 203

Query: 195 SVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVEGDMFT 254
            VF+ +ES VDVGGGTGTVAKAI+  FP+MECTV DLP VV  L+G + N K+V GDMF 
Sbjct: 204 WVFDELESFVDVGGGTGTVAKAIAATFPKMECTVFDLPHVVGDLQG-KDNLKFVGGDMFQ 263

Query: 255 LIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKNDADSI 314
            +PPAD ILLKWILHDWSD+ECV+ILKKCKEAIT  GSKKGKV++ID+++ N   D  S 
Sbjct: 264 QVPPADAILLKWILHDWSDDECVKILKKCKEAITSKGSKKGKVIIIDIMIENKDEDDKSY 323

Query: 315 ETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 358
           ETQL +DM+MM++  G+ER EKEWAKL +++GF  YKI PIL LRSLIE+YP
Sbjct: 324 ETQLFFDMMMMVLATGQERNEKEWAKLFQDSGFSDYKIIPILGLRSLIEVYP 373

BLAST of Cucsa.148920 vs. TrEMBL
Match: V4THS2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10015708mg PE=3 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 6.9e-125
Identity = 224/354 (63.28%), Postives = 274/354 (77.40%), Query Frame = 1

Query: 15  ELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSLLVSSLQLHPNKTQF 74
           ELLEAQ+HVWNHIFNFINSMSLKCA++LGIPD I+ HG  P+ L+ LVS+L ++P+KT+ 
Sbjct: 11  ELLEAQAHVWNHIFNFINSMSLKCAVELGIPDLINKHG-KPMTLNELVSALTINPSKTRC 70

Query: 75  IYRLMRLLTHSGFFVQQ-----------EEGYILTNSSRLLLKDNPCAVSPFLLSMLQPA 134
           +YRLMR+L HSGFF QQ           E+GY+LTN+S+LLLKDNP +V+PFL +ML P 
Sbjct: 71  VYRLMRILIHSGFFAQQTLNSSRNNNDEEQGYVLTNASKLLLKDNPLSVTPFLQAMLDPI 130

Query: 135 LTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVLLEK 194
           L  PW  LS WFQ DD TPF+TAHG  FW+Y  ++PK    FN  MASDARL  +V++ K
Sbjct: 131 LLSPWLKLSTWFQNDDPTPFDTAHGKSFWEYAGDEPKINNFFNEAMASDARLATSVVIHK 190

Query: 195 HRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVEGDM 254
            + VFEG+ SLVDVGGGTGTVAKAI++AFP +ECT  DLP VV  L+ D  N KYV GD+
Sbjct: 191 CKDVFEGLNSLVDVGGGTGTVAKAIAKAFPNLECTDFDLPHVVNGLESDLANLKYVGGDV 250

Query: 255 FTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKNDAD 314
           F  IPPAD +L KWILHDW+DEECV+IL+KCKEAIT S  KKGKV++ID++  N K D++
Sbjct: 251 FEAIPPADAVLFKWILHDWNDEECVKILQKCKEAIT-SDVKKGKVIIIDMMRENKKGDSE 310

Query: 315 SIETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 358
           SIETQL +DMLMM++  G ER+EKEWAKL  +AGF  YKI PIL LRSLIE+YP
Sbjct: 311 SIETQLFFDMLMMVLVTGTERDEKEWAKLFADAGFSDYKITPILGLRSLIEVYP 362

BLAST of Cucsa.148920 vs. TrEMBL
Match: F5A838_ROSCH (Orcinol O-methyltransferase-like protein OS=Rosa chinensis PE=3 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 6.5e-123
Identity = 221/350 (63.14%), Postives = 273/350 (78.00%), Query Frame = 1

Query: 14  DELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSLLVSSLQLHPNKTQ 73
           +ELL AQ+H+WNHIF+FINSMSLK AIQLGIPD I+ HG  P+ LS L S+L +HP K+ 
Sbjct: 21  NELLHAQAHIWNHIFSFINSMSLKSAIQLGIPDIINKHG-YPMTLSELTSALPIHPTKSH 80

Query: 74  FIYRLMRLLTHSGFFVQQ------EEGYILTNSSRLLLKDNPCAVSPFLLSMLQPALTDP 133
            +YRLMR+L HSGFF ++      EEGY LT++S+LLLKD+P +++PFL +ML P LT P
Sbjct: 81  SVYRLMRILVHSGFFAKKKLSKTDEEGYTLTDASQLLLKDHPLSLTPFLTAMLDPVLTKP 140

Query: 134 WQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVLLEKHRSV 193
           W +LS WFQ DD TPF+TAHG+ FW Y  ++P     FN  MASDARLV +V+++  + V
Sbjct: 141 WNYLSTWFQNDDPTPFDTAHGMTFWDYGNHQPSIAHFFNDAMASDARLVTSVIIDDCKGV 200

Query: 194 FEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVEGDMFTLI 253
           FEG+ESLVDVGGGTGTVAKAI+ AFP +ECTVLDLP VVA L+G + N KY  GDMF  +
Sbjct: 201 FEGLESLVDVGGGTGTVAKAIADAFPHIECTVLDLPHVVADLQGSK-NLKYTGGDMFEAV 260

Query: 254 PPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKNDADSIET 313
           PPADT+LLKWILHDW+DEECV+ILK+ +EAIT S  K GKV++ID+++ N K D +SIET
Sbjct: 261 PPADTVLLKWILHDWNDEECVKILKRSREAIT-SKDKNGKVIIIDMMMENQKGDEESIET 320

Query: 314 QLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 358
           QL +DMLMM +  GKER EKEWAKL  +AGF  YKI PIL LRSLIE+YP
Sbjct: 321 QLFFDMLMMALVTGKERNEKEWAKLFTDAGFSDYKITPILGLRSLIEVYP 367

BLAST of Cucsa.148920 vs. TAIR10
Match: AT4G35160.1 (AT4G35160.1 O-methyltransferase family protein)

HSP 1 Score: 206.5 bits (524), Expect = 2.8e-53
Identity = 128/364 (35.16%), Postives = 193/364 (53.02%), Query Frame = 1

Query: 14  DELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSH-GPNPVPLSLLVSSLQLHPNKT 73
           DE  +A   +W ++F F +  + KCAI L IP+AI +H    PV L+ L S++   P+  
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSHL 83

Query: 74  QFIYRLMRLLTHSGFFVQ------QEEGYILTNSSRLLL--KDNPCAVSPFLLSMLQPAL 133
           +   R+MR L H G F +         GY+ T  SR L+  + +  +++PF+L    P +
Sbjct: 84  R---RIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEM 143

Query: 134 TDPWQFLSIWFQT----DDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVL 193
             PW  LS    +        PF+  HG   W + ++ P   ++ N  MA DAR V+  +
Sbjct: 144 LAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRVVPRV 203

Query: 194 LEKHRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVE 253
                 +F+GV ++VDVGGGTG     + + FP ++    DLP V+  +       + VE
Sbjct: 204 AGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVI-EVAEVLDGVENVE 263

Query: 254 GDMFTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTK- 313
           GDMF  IP  D I +KW+LHDW D++C++ILK CKEA+       GKV++++ V+   K 
Sbjct: 264 GDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVP---PNIGKVLIVESVIGENKK 323

Query: 314 ------NDADSIETQLLYDMLMMI-VPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSL 357
                  D      +L+ DM+MM     GKER  KEW  ++KEAGF  Y++  I D++SL
Sbjct: 324 TMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSL 380

BLAST of Cucsa.148920 vs. TAIR10
Match: AT5G54160.1 (AT5G54160.1 O-methyltransferase 1)

HSP 1 Score: 156.4 bits (394), Expect = 3.4e-38
Identity = 101/321 (31.46%), Postives = 161/321 (50.16%), Query Frame = 1

Query: 34  MSLKCAIQLGIPDAIHSHGPNPVPLSLLVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQ-- 93
           M+LK A++L + + +  +G    P  +       +P     + R++RLLT          
Sbjct: 34  MALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNR 93

Query: 94  -------EEGYILTNSSRLLLK-DNPCAVSPFLLSMLQPALTDPWQFLSIWFQTDDQTPF 153
                  E  Y L    + L K ++  +++   L      L + W  L      D   PF
Sbjct: 94  KLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAI-LDGGIPF 153

Query: 154 ETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVLLEKHRSVFEGVESLVDVGGGTGT 213
             A+G+  ++Y    P+  +VFN GM++ + + +  +LE ++  FEG+ SLVDVGGG G 
Sbjct: 154 NKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG-FEGLTSLVDVGGGIGA 213

Query: 214 VAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVEGDMFTLIPPADTILLKWILHDWS 273
             K I   +P ++    DLP V+       P  ++V GDMF  +P  D I +KWI HDWS
Sbjct: 214 TLKMIVSKYPNLKGINFDLPHVIEDAPS-HPGIEHVGGDMFVSVPKGDAIFMKWICHDWS 273

Query: 274 DEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKNDADSIETQLLYDMLMMI-VPGGK 333
           DE CV+ LK C E++     + GKV++ + +L  T + + S +  +  D +M+   PGGK
Sbjct: 274 DEHCVKFLKNCYESL----PEDGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGK 333

Query: 334 EREEKEWAKLIKEAGFRAYKI 344
           ER EKE+  L K +GF+  K+
Sbjct: 334 ERTEKEFEALAKASGFKGIKV 347

BLAST of Cucsa.148920 vs. TAIR10
Match: AT1G51990.1 (AT1G51990.1 O-methyltransferase family protein)

HSP 1 Score: 154.8 bits (390), Expect = 9.8e-38
Identity = 102/313 (32.59%), Postives = 164/313 (52.40%), Query Frame = 1

Query: 57  PLSLLVSSLQLHPNKTQFIYRLMRLLTHSGF----FVQQEEG-----YILTNSSRLLLKD 116
           P+ L   +   +P+    I RL+R L          V+ EEG     Y L    + L+KD
Sbjct: 56  PVDLASMAAPKNPHAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVGKKLIKD 115

Query: 117 NP-CAVSPFLLSMLQPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFN 176
               +++P++L+    A    W +L+   Q    + +E A+    ++YM+      ++FN
Sbjct: 116 EDGFSIAPYVLAGCTKAKGGVWSYLTEAIQEGGASAWERANEALIFEYMKKNENLKKIFN 175

Query: 177 AGMASDARLVINVLLEKHRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVV 236
             M +   +V+  +LE +   FEGV   VDVGG  G+    I   +P ++    DLP +V
Sbjct: 176 ESMTNHTSIVMKKILENYIG-FEGVSDFVDVGGSLGSNLAQILSKYPHIKGINFDLPHIV 235

Query: 237 AHLKGDQPNFKYVEGDMFTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKG 296
                     +++ GDMF  IP  + IL+KWILHDW+DE+CVEILK CK+A+     + G
Sbjct: 236 KEAPQIH-GVEHIGGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCKKAL----PETG 295

Query: 297 KVMVIDLVLFNTKNDAD-SIETQLLYDMLMM-IVPGGKEREEKEWAKLIKEAGFRAYKIF 356
           +++VI++++    ++ D + +  L  D+ MM +  GGKER +KE+  L KEAGF+  KI 
Sbjct: 296 RIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSGGKERTKKEFEDLAKEAGFKLPKII 355

Query: 357 PILDLRSLIEIYP 358
                  +IE+YP
Sbjct: 356 YGAYSYWIIELYP 362

BLAST of Cucsa.148920 vs. TAIR10
Match: AT4G35150.1 (AT4G35150.1 O-methyltransferase family protein)

HSP 1 Score: 144.8 bits (364), Expect = 1.0e-34
Identity = 81/218 (37.16%), Postives = 125/218 (57.34%), Query Frame = 1

Query: 147 HGVPFWKYMRNKPKEGEVFNAGMASDARLVINVLLEKHRSVFEGVESLVDVGGGTGTVAK 206
           HG   W + ++     ++ N  MA DAR V+  +    + +F+GV ++VDVGGGTG    
Sbjct: 110 HGKDLWAFAQDNLCHSQLINEAMACDARRVVPRVAGACQGLFDGVATVVDVGGGTGETMG 169

Query: 207 AISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVEGDMFTLIPPADTILLKWILHDWSDEE 266
            + + FP ++    DLP V+  +       + VEGDMF  IP +D +++KW+LHDW D++
Sbjct: 170 ILVKEFPWIKGFNFDLPHVI-EVAQVLDGVENVEGDMFDSIPASDAVIIKWVLHDWGDKD 229

Query: 267 CVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKNDA------DSIE-TQLLYDMLMMI-V 326
           C++ILK CKEA+       GKV++++ V+   KN        D +E  +L  DM+MM+  
Sbjct: 230 CIKILKNCKEAVL---PNIGKVLIVECVIGEKKNTMIAEERDDKLEHVRLQLDMVMMVHT 289

Query: 327 PGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIY 357
             GKER  KEW  ++ EAGF  Y++    D++SLI  Y
Sbjct: 290 STGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLIIAY 323


HSP 2 Score: 55.8 bits (133), Expect = 6.2e-08
Identity = 30/76 (39.47%), Postives = 43/76 (56.58%), Query Frame = 1

Query: 14 DELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSH-GPNPVPLSLLVSSLQLHPNKT 73
          DE  +A   +W ++F F +  + KCAI L IP+AI +H    PV LS L S++   P+  
Sbjct: 10 DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPS-- 69

Query: 74 QFIYRLMRLLTHSGFF 89
            + R+MR L H G F
Sbjct: 70 -HLRRIMRFLVHQGLF 82

BLAST of Cucsa.148920 vs. TAIR10
Match: AT1G77530.1 (AT1G77530.1 O-methyltransferase family protein)

HSP 1 Score: 142.1 bits (357), Expect = 6.5e-34
Identity = 104/344 (30.23%), Postives = 178/344 (51.74%), Query Frame = 1

Query: 34  MSLKCAIQLGIPDAIH--SHGP--NPVPLSLLVSSLQLHPNKTQFIYRLMRLL-THS--- 93
           M LK A++LG+ D I   S+G   +P  +++ + +   +P     + R++RLL +HS   
Sbjct: 44  MVLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNKPTNPEAPVLLDRMLRLLVSHSILK 103

Query: 94  ---------GFFVQQEEGYILTNSSRLLLKDNPCA--VSPFLLSMLQPALTDPWQFLSIW 153
                    G   + E  Y      +  LKD+  +  +S  LL +    +   W  L   
Sbjct: 104 CCMVESRENGQTGKIERVYAAEPICKYFLKDSDGSGSLSSLLLLLHSQVILKTWTNLKDV 163

Query: 154 FQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVLLEKHRSVFEGVESL 213
              + +  F +AH +  ++Y+ +  +  ++F+  M+  + +V+  +LE++R  FE V +L
Sbjct: 164 I-LEGKDAFSSAHDMRLFEYISSDDQFSKLFHRAMSESSTMVMKKVLEEYRG-FEDVNTL 223

Query: 214 VDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVEGDMFTLIPPADTIL 273
           VDVGGG GT+   I+  +P ++    DL QV+       P  K+V GDMF  +P  D I 
Sbjct: 224 VDVGGGIGTILGLITSKYPHIKGVNFDLAQVLTQAPF-YPGVKHVSGDMFIEVPKGDAIF 283

Query: 274 LKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVL-FNTKNDADSIETQLLYDM 333
           +KWILHDW DE+C++ILK C +++     +KGKV++++++     K +  S  T L  D+
Sbjct: 284 MKWILHDWGDEDCIKILKNCWKSL----PEKGKVIIVEMITPMEPKPNDFSCNTVLGMDL 343

Query: 334 LMMI-VPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIY 357
           LM+    GGKER   ++  L   +GF   +I  +    S+IE +
Sbjct: 344 LMLTQCSGGKERSLSQFENLAFASGFLLCEIICLSYSYSVIEFH 380

BLAST of Cucsa.148920 vs. NCBI nr
Match: gi|778723662|ref|XP_004151735.2| (PREDICTED: trans-resveratrol di-O-methyltransferase [Cucumis sativus])

HSP 1 Score: 726.1 bits (1873), Expect = 3.0e-206
Identity = 356/357 (99.72%), Postives = 357/357 (100.00%), Query Frame = 1

Query: 1   MNMEGGKMLAMGGDELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSL 60
           MNMEGGKMLAMGGDELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLS+
Sbjct: 1   MNMEGGKMLAMGGDELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSI 60

Query: 61  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 120
           LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML
Sbjct: 61  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 120

Query: 121 QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVL 180
           QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVL
Sbjct: 121 QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVL 180

Query: 181 LEKHRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVE 240
           LEKHRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVE
Sbjct: 181 LEKHRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVE 240

Query: 241 GDMFTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKN 300
           GDMFTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKN
Sbjct: 241 GDMFTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKN 300

Query: 301 DADSIETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 358
           DADSIETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP
Sbjct: 301 DADSIETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 357

BLAST of Cucsa.148920 vs. NCBI nr
Match: gi|778723655|ref|XP_004151089.2| (PREDICTED: trans-resveratrol di-O-methyltransferase-like [Cucumis sativus])

HSP 1 Score: 643.7 bits (1659), Expect = 2.0e-181
Identity = 315/357 (88.24%), Postives = 333/357 (93.28%), Query Frame = 1

Query: 1   MNMEGGKMLAMGGDELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSL 60
           MNMEGG MLAMGGDELLEAQSH+WNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLS+
Sbjct: 1   MNMEGGNMLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSI 60

Query: 61  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 120
           LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML
Sbjct: 61  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 120

Query: 121 QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVL 180
           QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLV +VL
Sbjct: 121 QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVNSVL 180

Query: 181 LEKHRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVE 240
           L KH+SVFEGV+SLVDVGGGTGT+AKAISQAFPQMECTV DLPQVVAHL+GDQPN  YVE
Sbjct: 181 LGKHKSVFEGVKSLVDVGGGTGTMAKAISQAFPQMECTVFDLPQVVAHLRGDQPNLNYVE 240

Query: 241 GDMFTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKN 300
           GDMF  IPPAD +LLKWILHDWSDEECVEILK CK AI  +G+ KGKVMVID+VLF    
Sbjct: 241 GDMFKRIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGN-KGKVMVIDIVLFGNYK 300

Query: 301 DADSIETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 358
             DS+ETQLL+DMLMM + GGKEREEKEWAKLIKEAGF +YKIFPI+ +RSL+EIYP
Sbjct: 301 -KDSMETQLLFDMLMMTLVGGKEREEKEWAKLIKEAGFGSYKIFPIMGVRSLVEIYP 355

BLAST of Cucsa.148920 vs. NCBI nr
Match: gi|700188247|gb|KGN43480.1| (hypothetical protein Csa_7G039280 [Cucumis sativus])

HSP 1 Score: 643.7 bits (1659), Expect = 2.0e-181
Identity = 315/357 (88.24%), Postives = 333/357 (93.28%), Query Frame = 1

Query: 1   MNMEGGKMLAMGGDELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSL 60
           MNMEGG MLAMGGDELLEAQSH+WNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLS+
Sbjct: 26  MNMEGGNMLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSI 85

Query: 61  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 120
           LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML
Sbjct: 86  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 145

Query: 121 QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVL 180
           QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLV +VL
Sbjct: 146 QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVNSVL 205

Query: 181 LEKHRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVE 240
           L KH+SVFEGV+SLVDVGGGTGT+AKAISQAFPQMECTV DLPQVVAHL+GDQPN  YVE
Sbjct: 206 LGKHKSVFEGVKSLVDVGGGTGTMAKAISQAFPQMECTVFDLPQVVAHLRGDQPNLNYVE 265

Query: 241 GDMFTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKN 300
           GDMF  IPPAD +LLKWILHDWSDEECVEILK CK AI  +G+ KGKVMVID+VLF    
Sbjct: 266 GDMFKRIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGN-KGKVMVIDIVLFGNYK 325

Query: 301 DADSIETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 358
             DS+ETQLL+DMLMM + GGKEREEKEWAKLIKEAGF +YKIFPI+ +RSL+EIYP
Sbjct: 326 -KDSMETQLLFDMLMMTLVGGKEREEKEWAKLIKEAGFGSYKIFPIMGVRSLVEIYP 380

BLAST of Cucsa.148920 vs. NCBI nr
Match: gi|659110928|ref|XP_008455485.1| (PREDICTED: trans-resveratrol di-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 627.1 bits (1616), Expect = 1.9e-176
Identity = 309/358 (86.31%), Postives = 333/358 (93.02%), Query Frame = 1

Query: 1   MNME-GGKMLAMGGDELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLS 60
           MNME GGK LAMGGDELLEAQSH+WNHIFNFINSMSLKCAIQLGIPDAIHSHGPNP+PLS
Sbjct: 30  MNMEAGGKKLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS 89

Query: 61  LLVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSM 120
           LLVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDN  AVSPFLLSM
Sbjct: 90  LLVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNHFAVSPFLLSM 149

Query: 121 LQPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINV 180
           LQPALTDPWQFLSIW QTDD+TPFETAHG+PFW+YM NK K+GEVFN GMASDARLV++V
Sbjct: 150 LQPALTDPWQFLSIWLQTDDRTPFETAHGMPFWEYMGNKAKDGEVFNEGMASDARLVMSV 209

Query: 181 LLEKHRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYV 240
           +LEKH+SVFEGVESLVDVGGGTGT+AKAISQAFPQMECTV DLPQVVAHLK DQPNFKYV
Sbjct: 210 ILEKHKSVFEGVESLVDVGGGTGTMAKAISQAFPQMECTVFDLPQVVAHLKEDQPNFKYV 269

Query: 241 EGDMFTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTK 300
           EGDMF LIPPAD +LLKWILHDWSDEECVEILK CK AIT +G  +GKVMVID+VLF  K
Sbjct: 270 EGDMFKLIPPADVLLLKWILHDWSDEECVEILKNCKAAITSNGD-RGKVMVIDIVLFGNK 329

Query: 301 NDADSIETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 358
              DS+ETQLL+DMLMM + GGKEREE++WA+LIKEAGFR+YKIFPI+ +RSLIEIYP
Sbjct: 330 K--DSMETQLLFDMLMMTLAGGKEREEEKWAELIKEAGFRSYKIFPIMGVRSLIEIYP 384

BLAST of Cucsa.148920 vs. NCBI nr
Match: gi|659109765|ref|XP_008454865.1| (PREDICTED: trans-resveratrol di-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 500.0 bits (1286), Expect = 3.5e-138
Identity = 251/352 (71.31%), Postives = 296/352 (84.09%), Query Frame = 1

Query: 14  DELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSLLVSSLQLHPNKTQ 73
           DELLEAQ+H+WNH+  F+NSMSLKCAIQLGIP+AIH HG NP+PLSLLVSSL LHP KTQ
Sbjct: 67  DELLEAQAHIWNHMAAFVNSMSLKCAIQLGIPNAIHRHGSNPMPLSLLVSSLHLHPTKTQ 126

Query: 74  FIYRLMRLLTHSGFFV------QQEEGYILTNSSRLLLKDNPCAVSPFLLSMLQPALTDP 133
            +YRLMRLLTHSGFFV      ++EEGY+LT SSRLL++DNPC+V+P + ++L P L +P
Sbjct: 127 SLYRLMRLLTHSGFFVLQKEDEEEEEGYMLTKSSRLLVEDNPCSVTPLIHALLHPKLVEP 186

Query: 134 WQFLSIWFQTDDQTP-FETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVLLEKHRS 193
           WQF S  FQTDD  P F+TAHG+PFW+YM N PK+GE+FNAGMASDARLV+++LLEKH+ 
Sbjct: 187 WQFFSALFQTDDGVPVFKTAHGMPFWEYMENNPKDGEIFNAGMASDARLVMSLLLEKHKY 246

Query: 194 VFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLK-GDQPNFKYVEGDMFT 253
           VFEG+ESLVDVGGGTGTVAKAIS+ FPQ+ECT  DLPQVVA+L   + PNFKYVEGDMF 
Sbjct: 247 VFEGIESLVDVGGGTGTVAKAISKVFPQIECTSFDLPQVVANLNHNNPPNFKYVEGDMFK 306

Query: 254 LIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKNDADSI 313
            IP AD ILLKWILHDWSDEEC +IL+K KEAIT +G KKGKVMVID+V+   K   D+I
Sbjct: 307 HIPSADAILLKWILHDWSDEECSKILEKSKEAITRNG-KKGKVMVIDMVVDPKK---DTI 366

Query: 314 ETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 358
           +TQLL D+ MM++ GGKEREE E A+LIK+AGF +YKIFPIL LRSLIEIYP
Sbjct: 367 KTQLLCDIFMMVLLGGKEREENELAQLIKQAGFGSYKIFPILGLRSLIEIYP 414

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ROMT_VITVI7.1e-11559.43Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera GN=ROMT PE=1 SV=2[more]
Q8OMT_MENPI1.4e-9951.268-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita GN=OMT2 PE=1 SV=1[more]
HTOMT_CATRO4.2e-9149.86Tabersonine 16-O-methyltransferase OS=Catharanthus roseus GN=16OMT PE=1 SV=1[more]
MOMT_CATRO7.9e-9048.86Myricetin O-methyltransferase OS=Catharanthus roseus PE=1 SV=1[more]
CVMT1_OCIBA7.9e-9049.16Chavicol O-methyltransferase OS=Ocimum basilicum GN=CVOMT1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K5D5_CUCSA2.1e-20699.72Uncharacterized protein OS=Cucumis sativus GN=Csa_7G039790 PE=3 SV=1[more]
A0A0A0K1E9_CUCSA1.4e-18188.24Uncharacterized protein OS=Cucumis sativus GN=Csa_7G039280 PE=3 SV=1[more]
W9R084_9ROSA8.1e-12662.788-hydroxyquercetin 8-O-methyltransferase OS=Morus notabilis GN=L484_014739 PE=3 ... [more]
V4THS2_9ROSI6.9e-12563.28Uncharacterized protein OS=Citrus clementina GN=CICLE_v10015708mg PE=3 SV=1[more]
F5A838_ROSCH6.5e-12363.14Orcinol O-methyltransferase-like protein OS=Rosa chinensis PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G35160.12.8e-5335.16 O-methyltransferase family protein[more]
AT5G54160.13.4e-3831.46 O-methyltransferase 1[more]
AT1G51990.19.8e-3832.59 O-methyltransferase family protein[more]
AT4G35150.11.0e-3437.16 O-methyltransferase family protein[more]
AT1G77530.16.5e-3430.23 O-methyltransferase family protein[more]
Match NameE-valueIdentityDescription
gi|778723662|ref|XP_004151735.2|3.0e-20699.72PREDICTED: trans-resveratrol di-O-methyltransferase [Cucumis sativus][more]
gi|778723655|ref|XP_004151089.2|2.0e-18188.24PREDICTED: trans-resveratrol di-O-methyltransferase-like [Cucumis sativus][more]
gi|700188247|gb|KGN43480.1|2.0e-18188.24hypothetical protein Csa_7G039280 [Cucumis sativus][more]
gi|659110928|ref|XP_008455485.1|1.9e-17686.31PREDICTED: trans-resveratrol di-O-methyltransferase-like [Cucumis melo][more]
gi|659109765|ref|XP_008454865.1|3.5e-13871.31PREDICTED: trans-resveratrol di-O-methyltransferase-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001077O_MeTrfase_2
IPR011991Winged helix-turn-helix DNA-binding domain
IPR012967Plant_MeTrfase_dimerisation
IPR016461O-MeTrfase_COMT
Vocabulary: Molecular Function
TermDefinition
GO:0008171O-methyltransferase activity
GO:0046983protein dimerization activity
GO:0008168methyltransferase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
cellular_component GO:0005575 cellular_component
molecular_function GO:0008171 O-methyltransferase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0008168 methyltransferase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.148920.1Cucsa.148920.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001077O-methyltransferase, family 2PFAMPF00891Methyltransf_2coord: 118..343
score: 1.3
IPR011991Winged helix-turn-helix DNA-binding domainGENE3DG3DSA:1.10.10.10coord: 14..104
score: 3.7
IPR011991Winged helix-turn-helix DNA-binding domainunknownSSF46785"Winged helix" DNA-binding domaincoord: 13..109
score: 7.07
IPR012967Plant methyltransferase dimerisationPFAMPF08100Dimerisationcoord: 34..83
score: 1.7
IPR016461O-methyltransferase COMT-typePIRPIRSF005739O-mtasecoord: 3..357
score: 8.4E
IPR016461O-methyltransferase COMT-typePROFILEPS51683SAM_OMT_IIcoord: 24..357
score: 79
NoneNo IPR availablePANTHERPTHR11746O-METHYLTRANSFERASEcoord: 15..357
score: 2.7E
NoneNo IPR availablePANTHERPTHR11746:SF83SUBFAMILY NOT NAMEDcoord: 15..357
score: 2.7E