Cucsa.142360 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.142360 vs. Swiss-Prot
Match: METE_CATRO (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Catharanthus roseus GN=METE PE=2 SV=1)

HSP 1 Score: 1364.7 bits (3531), Expect = 0.0e+00
Identity = 674/765 (88.10%), Postives = 729/765 (95.29%), Query Frame = 1

Query: 63  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 122
           MASHIVGYPR+GPKRELKFALESFWD KSSA+DLQKVAADLRSSIWKQMA+AGIK+IPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDKKSSAEDLQKVAADLRSSIWKQMADAGIKYIPSN 60

Query: 123 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 182
           TFSYYDQVLDTATMLGAVP RY + GGEIGFD YFSMARGNASVPAMEMTKWFDTNYHYI
Sbjct: 61  TFSYYDQVLDTATMLGAVPPRYNFAGGEIGFDTYFSMARGNASVPAMEMTKWFDTNYHYI 120

Query: 183 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 242
           VPEL PE  F+YASHKAVNE+KEAK LGV+TVPVLVGPV+FLLLSKPAKGVEK+F LLSL
Sbjct: 121 VPELGPEVNFSYASHKAVNEYKEAKELGVDTVPVLVGPVTFLLLSKPAKGVEKTFPLLSL 180

Query: 243 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGLNVLI 302
           +DKILPVYKEV+ ELKAAGA+WIQFDEPTLV DL++H L+AFT+AYSELESTLSGLNV++
Sbjct: 181 LDKILPVYKEVIGELKAAGASWIQFDEPTLVLDLESHQLEAFTKAYSELESTLSGLNVIV 240

Query: 303 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDGRNIWS 362
           ETYFAD+PAE YK LT+LKGV+G+GFDLVRG +T+DLIKGGFPSGKYLFAGVVDGRNIW+
Sbjct: 241 ETYFADIPAETYKILTALKGVTGFGFDLVRGAKTLDLIKGGFPSGKYLFAGVVDGRNIWA 300

Query: 363 NDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVE 422
           NDL AS++TL++LE +VGK+K+VVSTSCSLLHTAVDLVNE KLD EIKSWLAFAAQKVVE
Sbjct: 301 NDLAASLSTLQSLEGIVGKDKLVVSTSCSLLHTAVDLVNEPKLDKEIKSWLAFAAQKVVE 360

Query: 423 VNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSARL 482
           VNALAKAL+G KDEA+FS NAAAQASRKSSPRVTN+AVQKAAAAL+GSDHRR+T VSARL
Sbjct: 361 VNALAKALAGEKDEAFFSENAAAQASRKSSPRVTNQAVQKAAAALRGSDHRRATTVSARL 420

Query: 483 DAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQEE 542
           DAQQKKLNLP+LPTTTIGSFPQT++LRRVRRE+KAKKISE+DYV +IKEEI+KVV+LQEE
Sbjct: 421 DAQQKKLNLPVLPTTTIGSFPQTLELRRVRREYKAKKISEDDYVKAIKEEISKVVKLQEE 480

Query: 543 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTV 602
           LDIDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPPIIYGDVSRP PMTV
Sbjct: 481 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPNPMTV 540

Query: 603 FWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIT 662
           FWS  AQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGI 
Sbjct: 541 FWSQTAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIN 600

Query: 663 VIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSI 722
           VIQIDEAALREGLPLRK+E  FYL+WAVHSFRITN  +QDTTQIHTHMCYSNFNDIIHSI
Sbjct: 601 VIQIDEAALREGLPLRKAEHAFYLDWAVHSFRITNLPLQDTTQIHTHMCYSNFNDIIHSI 660

Query: 723 IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLA 782
           IDMDADV+TIENSRS EKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLA
Sbjct: 661 IDMDADVMTIENSRSSEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLA 720

Query: 783 VLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 828
           VL++NILWVNPDCGLKTRKY EVKPAL NMV+AAKL+R+QLASAK
Sbjct: 721 VLDTNILWVNPDCGLKTRKYAEVKPALENMVSAAKLIRTQLASAK 765

BLAST of Cucsa.142360 vs. Swiss-Prot
Match: METE1_ORYSJ (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 OS=Oryza sativa subsp. japonica GN=Os12g0623900 PE=2 SV=1)

HSP 1 Score: 1357.0 bits (3511), Expect = 0.0e+00
Identity = 667/766 (87.08%), Postives = 726/766 (94.78%), Query Frame = 1

Query: 63  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 122
           MASHIVGYPR+GPKRELKFALESFWDGKSSA+DL+KVA DLR+SIWKQMA+AGIK+IPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSSAEDLEKVATDLRASIWKQMADAGIKYIPSN 60

Query: 123 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 182
           TFSYYDQVLDTA MLGAVP RY W GGEIGF  YFSMARGNA+VPAMEMTKWFDTNYH+I
Sbjct: 61  TFSYYDQVLDTAAMLGAVPERYSWTGGEIGFSTYFSMARGNATVPAMEMTKWFDTNYHFI 120

Query: 183 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 242
           VPEL P  KF+Y+SHKAVNE+KEAKALGV+TVPVLVGPVS+LLLSKPAKGVEKSF+LLSL
Sbjct: 121 VPELGPNTKFSYSSHKAVNEYKEAKALGVDTVPVLVGPVSYLLLSKPAKGVEKSFALLSL 180

Query: 243 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGLNVLI 302
           +  ILPVYKEV++ELKAAGATWIQFDEPTLV DLD+H L AF+ AY+ELES LSGLNVLI
Sbjct: 181 LSSILPVYKEVIAELKAAGATWIQFDEPTLVLDLDSHQLAAFSAAYTELESALSGLNVLI 240

Query: 303 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKG-GFPSGKYLFAGVVDGRNIW 362
           ETYFAD+PAE+YKTLTSL  V+ YGFDL+RG +T+DLIK  GFPSGKYLFAGVVDGRNIW
Sbjct: 241 ETYFADIPAESYKTLTSLNSVTAYGFDLIRGAKTLDLIKSAGFPSGKYLFAGVVDGRNIW 300

Query: 363 SNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVV 422
           ++DL AS+TTLE+LE +VGK+K+VVSTSCSL+HTAVDLVNETKLD EIKSWLAFAAQKVV
Sbjct: 301 ADDLAASLTTLESLEAIVGKDKLVVSTSCSLMHTAVDLVNETKLDSEIKSWLAFAAQKVV 360

Query: 423 EVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSAR 482
           EVNALAKAL+G KDEAYF++NAAAQASR+SSPRVTNE VQKAAAALKGSDHRR+TNVSAR
Sbjct: 361 EVNALAKALAGQKDEAYFAANAAAQASRRSSPRVTNEEVQKAAAALKGSDHRRATNVSAR 420

Query: 483 LDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQE 542
           LDAQQKKLNLP+LPTTTIGSFPQT++LRRVRRE+KAKKISEE+Y+S+IKEEI+KVV++QE
Sbjct: 421 LDAQQKKLNLPVLPTTTIGSFPQTVELRRVRREYKAKKISEEEYISAIKEEISKVVKIQE 480

Query: 543 ELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMT 602
           ELDIDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPPIIYGDVSRP PMT
Sbjct: 481 ELDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPNPMT 540

Query: 603 VFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGI 662
           VFWS +AQSMT RPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIK EVEDLE  GI
Sbjct: 541 VFWSKLAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKKEVEDLEAGGI 600

Query: 663 TVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHS 722
            VIQIDEAALREGLPLRK+E  FYL+WAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHS
Sbjct: 601 QVIQIDEAALREGLPLRKAEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHS 660

Query: 723 IIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKML 782
           II+MDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADR+NKML
Sbjct: 661 IINMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRVNKML 720

Query: 783 AVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 828
           AVL++NILWVNPDCGLKTRKY EVKPAL NMV+AAKL+R+QLASAK
Sbjct: 721 AVLDTNILWVNPDCGLKTRKYNEVKPALTNMVSAAKLIRTQLASAK 766

BLAST of Cucsa.142360 vs. Swiss-Prot
Match: METE1_ARATH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 OS=Arabidopsis thaliana GN=MS1 PE=1 SV=1)

HSP 1 Score: 1355.5 bits (3507), Expect = 0.0e+00
Identity = 668/765 (87.32%), Postives = 720/765 (94.12%), Query Frame = 1

Query: 63  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 122
           MASHIVGYPR+GPKRELKFALESFWDGKS+A+DLQKV+ADLRSSIWKQM+ AG KFIPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSTAEDLQKVSADLRSSIWKQMSAAGTKFIPSN 60

Query: 123 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 182
           TF++YDQVLDT  MLGAVP RYG+ GGEIG D+YFSMARGNASVPAMEMTKWFDTNYHYI
Sbjct: 61  TFAHYDQVLDTTAMLGAVPPRYGYTGGEIGLDVYFSMARGNASVPAMEMTKWFDTNYHYI 120

Query: 183 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 242
           VPEL PE  F+YASHKAVNE+KEAKALGV+TVPVLVGPVS+LLLSK AKGV+KSF LLSL
Sbjct: 121 VPELGPEVNFSYASHKAVNEYKEAKALGVDTVPVLVGPVSYLLLSKAAKGVDKSFELLSL 180

Query: 243 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGLNVLI 302
           + KILP+YKEV++ELKAAGATWIQ DEP LV DL+   LQAFT AY+ELESTLSGLNVL+
Sbjct: 181 LPKILPIYKEVITELKAAGATWIQLDEPVLVMDLEGQKLQAFTGAYAELESTLSGLNVLV 240

Query: 303 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDGRNIWS 362
           ETYFAD+PAEAYKTLTSLKGV+ +GFDLVRGT+T+DL+K GFP GKYLFAGVVDGRNIW+
Sbjct: 241 ETYFADIPAEAYKTLTSLKGVTAFGFDLVRGTKTLDLVKAGFPEGKYLFAGVVDGRNIWA 300

Query: 363 NDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVE 422
           ND  AS++TL+ALE +VGK+K+VVSTSCSLLHTAVDL+NETKLDDEIKSWLAFAAQKVVE
Sbjct: 301 NDFAASLSTLQALEGIVGKDKLVVSTSCSLLHTAVDLINETKLDDEIKSWLAFAAQKVVE 360

Query: 423 VNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSARL 482
           VNALAKAL+G KDEA FS+NAAA ASR+SSPRVTNE VQKAAAALKGSDHRR+TNVSARL
Sbjct: 361 VNALAKALAGQKDEALFSANAAALASRRSSPRVTNEGVQKAAAALKGSDHRRATNVSARL 420

Query: 483 DAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQEE 542
           DAQQKKLNLP+LPTTTIGSFPQT++LRRVRRE+KAKK+SEEDYV +IKEEI KVV LQEE
Sbjct: 421 DAQQKKLNLPILPTTTIGSFPQTVELRRVRREYKAKKVSEEDYVKAIKEEIKKVVDLQEE 480

Query: 543 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTV 602
           LDIDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPP+IYGDVSRPK MTV
Sbjct: 481 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPVIYGDVSRPKAMTV 540

Query: 603 FWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIT 662
           FWS MAQSMT RPMKGMLTGPVTILNWSFVRNDQPR ETCYQIALAIKDEVEDLEK GI 
Sbjct: 541 FWSAMAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKGGIG 600

Query: 663 VIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSI 722
           VIQIDEAALREGLPLRKSE  FYL+WAVHSFRITNCGVQD+TQIHTHMCYS+FNDIIHSI
Sbjct: 601 VIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDSTQIHTHMCYSHFNDIIHSI 660

Query: 723 IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLA 782
           IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPS+EEIADR+NKMLA
Sbjct: 661 IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSSEEIADRVNKMLA 720

Query: 783 VLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 828
           VLE NILWVNPDCGLKTRKYTEVKPAL NMV AAKL+RSQLASAK
Sbjct: 721 VLEQNILWVNPDCGLKTRKYTEVKPALKNMVDAAKLIRSQLASAK 765

BLAST of Cucsa.142360 vs. Swiss-Prot
Match: METE2_ORYSJ (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 2 OS=Oryza sativa subsp. japonica GN=Os12g0624000 PE=2 SV=1)

HSP 1 Score: 1351.7 bits (3497), Expect = 0.0e+00
Identity = 665/766 (86.81%), Postives = 724/766 (94.52%), Query Frame = 1

Query: 63  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 122
           MASHIVGYPR+GPKRELKFALESFWDGKSSA+DL+KVA DLR+SIWKQMA+AGIK+IPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSSAEDLEKVATDLRASIWKQMADAGIKYIPSN 60

Query: 123 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 182
           TFSYYDQVLDT  MLGAVP RY W GGEIGF  YFSMARGNA+VPAMEMTKWFDTNYH+I
Sbjct: 61  TFSYYDQVLDTTAMLGAVPERYSWTGGEIGFSTYFSMARGNATVPAMEMTKWFDTNYHFI 120

Query: 183 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 242
           VPEL P  KF+Y+SHKAVNE+KEAKALGV+TVPVLVGPVS+LLLSKPAKGVEKSF+LLSL
Sbjct: 121 VPELGPNTKFSYSSHKAVNEYKEAKALGVDTVPVLVGPVSYLLLSKPAKGVEKSFALLSL 180

Query: 243 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGLNVLI 302
           +  ILPVYKEV++ELKAAGATWIQFDEPTLV DLD+H L AF+ AY+ELES LSGLNVLI
Sbjct: 181 LSSILPVYKEVIAELKAAGATWIQFDEPTLVLDLDSHQLAAFSAAYTELESALSGLNVLI 240

Query: 303 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKG-GFPSGKYLFAGVVDGRNIW 362
           ETYFAD+PAE+YKTLTSL  V+ YGFDL+RG++T+DL+K  GFPSGKYLFAGVVDGRNIW
Sbjct: 241 ETYFADIPAESYKTLTSLNSVTAYGFDLIRGSKTLDLVKSAGFPSGKYLFAGVVDGRNIW 300

Query: 363 SNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVV 422
           ++DL AS+TTLE+LE +VGK+K+VVSTSCSL+HTAVDLVNETKLD EIKSWLAFAAQKVV
Sbjct: 301 ADDLAASLTTLESLEAIVGKDKLVVSTSCSLMHTAVDLVNETKLDSEIKSWLAFAAQKVV 360

Query: 423 EVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSAR 482
           EVNALAKAL+G KDEAYF++N AAQASR+SSPRVTNE VQKAAAAL+GSDHRR+TNVSAR
Sbjct: 361 EVNALAKALAGQKDEAYFAANTAAQASRRSSPRVTNEEVQKAAAALRGSDHRRATNVSAR 420

Query: 483 LDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQE 542
           LDAQQKKLNLP+LPTTTIGSFPQT++LRRVRRE+KAKKISE++YVS+IKEEI+KVV++QE
Sbjct: 421 LDAQQKKLNLPVLPTTTIGSFPQTVELRRVRREYKAKKISEDEYVSAIKEEISKVVKIQE 480

Query: 543 ELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMT 602
           ELDIDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPPIIYGDVSRP  MT
Sbjct: 481 ELDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPNAMT 540

Query: 603 VFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGI 662
           VFWS MAQSMT RPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIK EVEDLE  GI
Sbjct: 541 VFWSKMAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKKEVEDLEAGGI 600

Query: 663 TVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHS 722
            VIQIDEAALREGLPLRK+E  FYL+WAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHS
Sbjct: 601 QVIQIDEAALREGLPLRKAEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHS 660

Query: 723 IIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKML 782
           II+MDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKML
Sbjct: 661 IINMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKML 720

Query: 783 AVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 828
           AVL++NILWVNPDCGLKTRKYTEVKPAL NMV AAKL+R+QLASAK
Sbjct: 721 AVLDTNILWVNPDCGLKTRKYTEVKPALTNMVLAAKLIRTQLASAK 766

BLAST of Cucsa.142360 vs. Swiss-Prot
Match: METE_PLESU (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Plectranthus scutellarioides GN=MET PE=1 SV=2)

HSP 1 Score: 1347.4 bits (3486), Expect = 0.0e+00
Identity = 669/765 (87.45%), Postives = 722/765 (94.38%), Query Frame = 1

Query: 63  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 122
           MASHIVGYPR+GPKRELKFALESFWDGKSSA+DL+K    L   + KQM++AGIK+IPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSSAEDLEKCQLILGIHL-KQMSDAGIKYIPSN 60

Query: 123 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 182
           TFSYYDQVLDT  MLGAVP RY W GGEIGF  YFSMARGNASVPAMEMTKWFDTNYH+I
Sbjct: 61  TFSYYDQVLDTTAMLGAVPPRYNWTGGEIGFSTYFSMARGNASVPAMEMTKWFDTNYHFI 120

Query: 183 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 242
           VPEL P+ KF+YASHKAVNE+KEAKALGV+TVPVLVGPVS+L+LSKPAKGVEK+F LLSL
Sbjct: 121 VPELGPDVKFSYASHKAVNEYKEAKALGVDTVPVLVGPVSYLILSKPAKGVEKTFPLLSL 180

Query: 243 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGLNVLI 302
           +DKILP+YKEV++ELKAAGA+WIQFDEPTLV DL++H L AFT+AY+ELES+LSGL+ LI
Sbjct: 181 LDKILPIYKEVIAELKAAGASWIQFDEPTLVLDLESHQLDAFTKAYAELESSLSGLSTLI 240

Query: 303 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDGRNIWS 362
           ETYFADVPA AYKTLTSL G+SG+GFDLVRG QT++LIKGGFPSGKYLFAGVVDGRNIW+
Sbjct: 241 ETYFADVPAPAYKTLTSLSGISGFGFDLVRGAQTIELIKGGFPSGKYLFAGVVDGRNIWA 300

Query: 363 NDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVE 422
           NDL +SITTL+ALE +VGK+K+VVSTS SLLHTAVDLVNE KLD EIKSWLAFAAQK+VE
Sbjct: 301 NDLASSITTLQALEGIVGKDKLVVSTSSSLLHTAVDLVNEPKLDQEIKSWLAFAAQKIVE 360

Query: 423 VNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSARL 482
           VNALAKAL+GHKDEA+FS NAAAQASRKSSPRV NEAVQKAAAAL+GS+HRR TNVSARL
Sbjct: 361 VNALAKALTGHKDEAFFSPNAAAQASRKSSPRVNNEAVQKAAAALRGSEHRRVTNVSARL 420

Query: 483 DAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQEE 542
           DAQQKKLNLP+LPTTTIGSFPQT++LRRVRREFK  +ISEE+YV +IKEEINKVV+LQEE
Sbjct: 421 DAQQKKLNLPILPTTTIGSFPQTVELRRVRREFKPTRISEEEYVKAIKEEINKVVKLQEE 480

Query: 543 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTV 602
           LDIDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPPIIYGDVSRPKPMTV
Sbjct: 481 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKPMTV 540

Query: 603 FWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIT 662
           FWST AQSMT+RPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIT
Sbjct: 541 FWSTAAQSMTQRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIT 600

Query: 663 VIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSI 722
           VIQIDEAALREGLPLRKSE  FYL+WAVHSFRITN GVQDTTQIHTHMCYSNFNDIIHSI
Sbjct: 601 VIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNVGVQDTTQIHTHMCYSNFNDIIHSI 660

Query: 723 IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLA 782
           I+MDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLA
Sbjct: 661 INMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLA 720

Query: 783 VLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 828
           VLE+NILWVNPDCGLKTRKY EVKPAL NMVAAAKLLR+QLASAK
Sbjct: 721 VLETNILWVNPDCGLKTRKYAEVKPALENMVAAAKLLRTQLASAK 764

BLAST of Cucsa.142360 vs. TrEMBL
Match: A0A0A0LZR2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G599580 PE=3 SV=1)

HSP 1 Score: 1629.0 bits (4217), Expect = 0.0e+00
Identity = 827/827 (100.00%), Postives = 827/827 (100.00%), Query Frame = 1

Query: 1   MTQLPLKSLEPFAISSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRSTSTVV 60
           MTQLPLKSLEPFAISSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRSTSTVV
Sbjct: 1   MTQLPLKSLEPFAISSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRSTSTVV 60

Query: 61  RAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIP 120
           RAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIP
Sbjct: 61  RAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIP 120

Query: 121 SNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYH 180
           SNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYH
Sbjct: 121 SNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYH 180

Query: 181 YIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLL 240
           YIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLL
Sbjct: 181 YIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLL 240

Query: 241 SLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGLNV 300
           SLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGLNV
Sbjct: 241 SLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGLNV 300

Query: 301 LIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDGRNI 360
           LIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDGRNI
Sbjct: 301 LIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDGRNI 360

Query: 361 WSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKV 420
           WSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKV
Sbjct: 361 WSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKV 420

Query: 421 VEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSA 480
           VEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSA
Sbjct: 421 VEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSA 480

Query: 481 RLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQ 540
           RLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQ
Sbjct: 481 RLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQ 540

Query: 541 EELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPM 600
           EELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPM
Sbjct: 541 EELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPM 600

Query: 601 TVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAG 660
           TVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAG
Sbjct: 601 TVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAG 660

Query: 661 ITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIH 720
           ITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIH
Sbjct: 661 ITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIH 720

Query: 721 SIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKM 780
           SIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKM
Sbjct: 721 SIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKM 780

Query: 781 LAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 828
           LAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK
Sbjct: 781 LAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 827

BLAST of Cucsa.142360 vs. TrEMBL
Match: W9R640_9ROSA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Morus notabilis GN=L484_009365 PE=3 SV=1)

HSP 1 Score: 1413.3 bits (3657), Expect = 0.0e+00
Identity = 701/765 (91.63%), Postives = 742/765 (96.99%), Query Frame = 1

Query: 63  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 122
           MASHIVGYPR+GPKRELKFALESFWDGKSSA+DLQKVAADLR+SIWKQM+EAGIK+IPSN
Sbjct: 1   MASHIVGYPRVGPKRELKFALESFWDGKSSAEDLQKVAADLRASIWKQMSEAGIKYIPSN 60

Query: 123 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 182
           TFSYYDQVLDT  MLGAVP RYGWNGGEIGFD+YFSMARGNASVPAMEMTKWFDTNYHYI
Sbjct: 61  TFSYYDQVLDTTAMLGAVPPRYGWNGGEIGFDVYFSMARGNASVPAMEMTKWFDTNYHYI 120

Query: 183 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 242
           VPELSPE KF+YASHKAV E+KEAKALGVETVPVLVGPVS+LLLSKPAKGVEKSFSLLSL
Sbjct: 121 VPELSPEIKFSYASHKAVEEYKEAKALGVETVPVLVGPVSYLLLSKPAKGVEKSFSLLSL 180

Query: 243 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGLNVLI 302
           I  ILPVYKEV++ELKAAGATW+QFDEPTLVKDLDAH LQAFT AYSELES+LSGLNV+I
Sbjct: 181 IGSILPVYKEVLAELKAAGATWVQFDEPTLVKDLDAHQLQAFTHAYSELESSLSGLNVVI 240

Query: 303 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDGRNIWS 362
           ETYFADV AEA+KTLT LKGV+GYGFDLVRGT+T+DLIKGGFPSGKYLFAGVVDGRNIW+
Sbjct: 241 ETYFADVTAEAFKTLTGLKGVTGYGFDLVRGTKTLDLIKGGFPSGKYLFAGVVDGRNIWA 300

Query: 363 NDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVE 422
           NDL +S++TLEALE +VGK+K+VVSTSCSLLHTAVDLVNETKLD EIKSWLAFAAQKVVE
Sbjct: 301 NDLASSLSTLEALEGIVGKDKLVVSTSCSLLHTAVDLVNETKLDKEIKSWLAFAAQKVVE 360

Query: 423 VNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSARL 482
           VNALAKAL+G KDEA+F++NA AQASR+SSPRVTNEAVQKAAAALKGSDHRR+TNVS+RL
Sbjct: 361 VNALAKALAGQKDEAFFTANAGAQASRRSSPRVTNEAVQKAAAALKGSDHRRATNVSSRL 420

Query: 483 DAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQEE 542
           DAQQKKLNLP LPTTTIGSFPQT++LRRVRRE+KAKKISEE+YV++IKEEI KVV+LQEE
Sbjct: 421 DAQQKKLNLPALPTTTIGSFPQTLELRRVRREYKAKKISEEEYVNAIKEEIKKVVKLQEE 480

Query: 543 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTV 602
           LDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTV
Sbjct: 481 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTV 540

Query: 603 FWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIT 662
           FWS+ AQS TKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIT
Sbjct: 541 FWSSFAQSTTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIT 600

Query: 663 VIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSI 722
           VIQIDEAALREGLPLRKSEE FYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSI
Sbjct: 601 VIQIDEAALREGLPLRKSEEAFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSI 660

Query: 723 IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLA 782
           IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPS EEIADRINKMLA
Sbjct: 661 IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSREEIADRINKMLA 720

Query: 783 VLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 828
           VLESNILWVNPDCGLKTRKY+EVKPAL NMVAAAKLLRSQLASAK
Sbjct: 721 VLESNILWVNPDCGLKTRKYSEVKPALSNMVAAAKLLRSQLASAK 765

BLAST of Cucsa.142360 vs. TrEMBL
Match: I1LXY1_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_13G028400 PE=3 SV=1)

HSP 1 Score: 1404.0 bits (3633), Expect = 0.0e+00
Identity = 700/796 (87.94%), Postives = 750/796 (94.22%), Query Frame = 1

Query: 32  IAPFSYLLFSSIHHLPSLRLSIRSTSTVVRAMASHIVGYPRIGPKRELKFALESFWDGKS 91
           +APF++ L +      SLR S+R+TS+  RAMASHIVGYPR+GPKRELKFALESFWDGKS
Sbjct: 10  VAPFTFSLCTLSFSSYSLRFSVRATSS--RAMASHIVGYPRMGPKRELKFALESFWDGKS 69

Query: 92  SADDLQKVAADLRSSIWKQMAEAGIKFIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEI 151
           SA++LQKVAADLRS+IWKQMA+AGIK+IPSNTFS YDQVLDT  MLGAVPSRY WNGGEI
Sbjct: 70  SAEELQKVAADLRSAIWKQMADAGIKYIPSNTFSLYDQVLDTTAMLGAVPSRYNWNGGEI 129

Query: 152 GFDIYFSMARGNASVPAMEMTKWFDTNYHYIVPELSPEDKFTYASHKAVNEFKEAKALGV 211
           GFD+YFSMARGNASVPAMEMTKWFDTNYHYIVPEL P+ KF+YASHKAV+EFKEAK LGV
Sbjct: 130 GFDVYFSMARGNASVPAMEMTKWFDTNYHYIVPELGPDVKFSYASHKAVDEFKEAKVLGV 189

Query: 212 ETVPVLVGPVSFLLLSKPAKGVEKSFSLLSLIDKILPVYKEVVSELKAAGATWIQFDEPT 271
            TVPVLVGPVS+LLLSKPAKGVEKSFSLLSLIDKILPVY+EVV+ELKAAGATWIQFDEPT
Sbjct: 190 NTVPVLVGPVSYLLLSKPAKGVEKSFSLLSLIDKILPVYREVVAELKAAGATWIQFDEPT 249

Query: 272 LVKDLDAHHLQAFTQAYSELESTLSGLNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLV 331
           LVKDL+ H LQAFT AY+ELES+LSG NVLIETYFADVPAEAYKTLTSLK V+ YGFD+V
Sbjct: 250 LVKDLNTHQLQAFTHAYAELESSLSGFNVLIETYFADVPAEAYKTLTSLKAVTAYGFDIV 309

Query: 332 RGTQTVDLIKGGFPSGKYLFAGVVDGRNIWSNDLQASITTLEALENVVGKEKIVVSTSCS 391
           RGT+T+DLIK GFPSGK+LFAGVVDGRNIW+N L +S+ TL+AL ++VG +K+VVSTSCS
Sbjct: 310 RGTKTLDLIKQGFPSGKFLFAGVVDGRNIWANSLASSLNTLQALGDIVGNDKVVVSTSCS 369

Query: 392 LLHTAVDLVNETKLDDEIKSWLAFAAQKVVEVNALAKALSGHKDEAYFSSNAAAQASRKS 451
           LLHTAVDLVNETKLD EIKSWLAFAAQKVVEVNALAKALSG KDE +FS+NAAA  SRKS
Sbjct: 370 LLHTAVDLVNETKLDQEIKSWLAFAAQKVVEVNALAKALSGQKDEVFFSANAAALDSRKS 429

Query: 452 SPRVTNEAVQKAAAALKGSDHRRSTNVSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRV 511
           SPRVTNEAVQKAAAALKGSDHRR+TNVSARLD+QQKKLNLP+LPTTTIGSFPQT DLRRV
Sbjct: 430 SPRVTNEAVQKAAAALKGSDHRRATNVSARLDSQQKKLNLPVLPTTTIGSFPQTADLRRV 489

Query: 512 RREFKAKKISEEDYVSSIKEEINKVVQLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAF 571
           RREFKA KISEEDY+  IKEEIN VV+LQEELDIDVLVHGEPERNDMVEYFGEQLSGFAF
Sbjct: 490 RREFKANKISEEDYIHFIKEEINNVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAF 549

Query: 572 TVNGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSF 631
           T NGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWS+ AQS+TKRPMKGMLTGPVTILNWSF
Sbjct: 550 TANGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWSSTAQSLTKRPMKGMLTGPVTILNWSF 609

Query: 632 VRNDQPRFETCYQIALAIKDEVEDLEKAGITVIQIDEAALREGLPLRKSEENFYLNWAVH 691
           VR+DQPRFETCYQIALAIKDEVEDLEKAGITVIQIDEAALREGLPLRKSEE FYLNWAVH
Sbjct: 610 VRDDQPRFETCYQIALAIKDEVEDLEKAGITVIQIDEAALREGLPLRKSEEAFYLNWAVH 669

Query: 692 SFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKY 751
           SFRITNCGV+DTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKY
Sbjct: 670 SFRITNCGVEDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKY 729

Query: 752 GAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLESNILWVNPDCGLKTRKYTEVKPALLN 811
           GAGIGPG+YDIHSPRIP TEEIADRINKMLAVLESNILWVNPDCGLKTRKYTEVKPAL N
Sbjct: 730 GAGIGPGIYDIHSPRIPPTEEIADRINKMLAVLESNILWVNPDCGLKTRKYTEVKPALTN 789

Query: 812 MVAAAKLLRSQLASAK 828
           MVAAAKL+R+QLASAK
Sbjct: 790 MVAAAKLIRNQLASAK 803

BLAST of Cucsa.142360 vs. TrEMBL
Match: A0A0B2SP06_GLYSO (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Glycine soja GN=glysoja_045875 PE=3 SV=1)

HSP 1 Score: 1404.0 bits (3633), Expect = 0.0e+00
Identity = 700/796 (87.94%), Postives = 750/796 (94.22%), Query Frame = 1

Query: 32  IAPFSYLLFSSIHHLPSLRLSIRSTSTVVRAMASHIVGYPRIGPKRELKFALESFWDGKS 91
           +APF++ L +      SLR S+R+TS+  RAMASHIVGYPR+GPKRELKFALESFWDGKS
Sbjct: 10  VAPFTFSLCTLSFSSYSLRFSVRATSS--RAMASHIVGYPRMGPKRELKFALESFWDGKS 69

Query: 92  SADDLQKVAADLRSSIWKQMAEAGIKFIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEI 151
           SA++LQKVAADLRS+IWKQMA+AGIK+IPSNTFS YDQVLDT  MLGAVPSRY WNGGEI
Sbjct: 70  SAEELQKVAADLRSAIWKQMADAGIKYIPSNTFSLYDQVLDTTAMLGAVPSRYNWNGGEI 129

Query: 152 GFDIYFSMARGNASVPAMEMTKWFDTNYHYIVPELSPEDKFTYASHKAVNEFKEAKALGV 211
           GFD+YFSMARGNASVPAMEMTKWFDTNYHYIVPEL P+ KF+YASHKAV+EFKEAK LGV
Sbjct: 130 GFDVYFSMARGNASVPAMEMTKWFDTNYHYIVPELGPDVKFSYASHKAVDEFKEAKVLGV 189

Query: 212 ETVPVLVGPVSFLLLSKPAKGVEKSFSLLSLIDKILPVYKEVVSELKAAGATWIQFDEPT 271
            TVPVLVGPVS+LLLSKPAKGVEKSFSLLSLIDKILPVY+EVV+ELKAAGATWIQFDEPT
Sbjct: 190 NTVPVLVGPVSYLLLSKPAKGVEKSFSLLSLIDKILPVYREVVAELKAAGATWIQFDEPT 249

Query: 272 LVKDLDAHHLQAFTQAYSELESTLSGLNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLV 331
           LVKDL+ H LQAFT AY+ELES+LSG NVLIETYFADVPAEAYKTLTSLK V+ YGFD+V
Sbjct: 250 LVKDLNTHQLQAFTHAYAELESSLSGFNVLIETYFADVPAEAYKTLTSLKAVTAYGFDIV 309

Query: 332 RGTQTVDLIKGGFPSGKYLFAGVVDGRNIWSNDLQASITTLEALENVVGKEKIVVSTSCS 391
           RGT+T+DLIK GFPSGK+LFAGVVDGRNIW+N L +S+ TL+AL ++VG +K+VVSTSCS
Sbjct: 310 RGTKTLDLIKQGFPSGKFLFAGVVDGRNIWANSLASSLNTLQALGDIVGNDKVVVSTSCS 369

Query: 392 LLHTAVDLVNETKLDDEIKSWLAFAAQKVVEVNALAKALSGHKDEAYFSSNAAAQASRKS 451
           LLHTAVDLVNETKLD EIKSWLAFAAQKVVEVNALAKALSG KDE +FS+NAAA  SRKS
Sbjct: 370 LLHTAVDLVNETKLDQEIKSWLAFAAQKVVEVNALAKALSGQKDEVFFSANAAALDSRKS 429

Query: 452 SPRVTNEAVQKAAAALKGSDHRRSTNVSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRV 511
           SPRVTNEAVQKAAAALKGSDHRR+TNVSARLD+QQKKLNLP+LPTTTIGSFPQT DLRRV
Sbjct: 430 SPRVTNEAVQKAAAALKGSDHRRATNVSARLDSQQKKLNLPVLPTTTIGSFPQTADLRRV 489

Query: 512 RREFKAKKISEEDYVSSIKEEINKVVQLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAF 571
           RREFKA KISEEDY+  IKEEIN VV+LQEELDIDVLVHGEPERNDMVEYFGEQLSGFAF
Sbjct: 490 RREFKANKISEEDYIHFIKEEINNVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAF 549

Query: 572 TVNGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSF 631
           T NGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWS+ AQS+TKRPMKGMLTGPVTILNWSF
Sbjct: 550 TANGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWSSTAQSLTKRPMKGMLTGPVTILNWSF 609

Query: 632 VRNDQPRFETCYQIALAIKDEVEDLEKAGITVIQIDEAALREGLPLRKSEENFYLNWAVH 691
           VR+DQPRFETCYQIALAIKDEVEDLEKAGITVIQIDEAALREGLPLRKSEE FYLNWAVH
Sbjct: 610 VRDDQPRFETCYQIALAIKDEVEDLEKAGITVIQIDEAALREGLPLRKSEEAFYLNWAVH 669

Query: 692 SFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKY 751
           SFRITNCGV+DTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKY
Sbjct: 670 SFRITNCGVEDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKY 729

Query: 752 GAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLESNILWVNPDCGLKTRKYTEVKPALLN 811
           GAGIGPG+YDIHSPRIP TEEIADRINKMLAVLESNILWVNPDCGLKTRKYTEVKPAL N
Sbjct: 730 GAGIGPGIYDIHSPRIPPTEEIADRINKMLAVLESNILWVNPDCGLKTRKYTEVKPALTN 789

Query: 812 MVAAAKLLRSQLASAK 828
           MVAAAKL+R+QLASAK
Sbjct: 790 MVAAAKLIRNQLASAK 803

BLAST of Cucsa.142360 vs. TrEMBL
Match: A0A059AAZ7_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_J00612 PE=3 SV=1)

HSP 1 Score: 1400.2 bits (3623), Expect = 0.0e+00
Identity = 698/782 (89.26%), Postives = 749/782 (95.78%), Query Frame = 1

Query: 46  LPSLRLSIRSTSTVVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRS 105
           L  LR ++R     VRAMASHIVGYPR+GPKRELKFALESFWD KSSA++LQKVAADLR+
Sbjct: 48  LSVLRFALRPPP--VRAMASHIVGYPRMGPKRELKFALESFWDRKSSAEELQKVAADLRA 107

Query: 106 SIWKQMAEAGIKFIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNAS 165
           SIWKQMA AGIK+IPSNTFSYYDQVLDT  MLGAVP RYGWNGGEI FD YFSMARGNAS
Sbjct: 108 SIWKQMASAGIKYIPSNTFSYYDQVLDTTAMLGAVPPRYGWNGGEIEFDTYFSMARGNAS 167

Query: 166 VPAMEMTKWFDTNYHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLL 225
           VPAMEMTKWFDTNYHYIVPEL P+ KF+YASHKAV+EFKEA+ALGV+TVPVLVGPVS+LL
Sbjct: 168 VPAMEMTKWFDTNYHYIVPELGPDVKFSYASHKAVDEFKEARALGVDTVPVLVGPVSYLL 227

Query: 226 LSKPAKGVEKSFSLLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFT 285
           LSKPAKGVEKSFSLLSLI KI+P+YKEV++ELKAAGATWIQFDEPTLVKDLD+H LQAFT
Sbjct: 228 LSKPAKGVEKSFSLLSLIGKIVPIYKEVIAELKAAGATWIQFDEPTLVKDLDSHQLQAFT 287

Query: 286 QAYSELESTLSGLNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFP 345
            AYSELES+LSGL++LIETYFADVPAEAYKTLTSLKG++G+GFDLVRGT+T+DL+KGGFP
Sbjct: 288 HAYSELESSLSGLSILIETYFADVPAEAYKTLTSLKGIAGFGFDLVRGTKTLDLVKGGFP 347

Query: 346 SGKYLFAGVVDGRNIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKL 405
           SGKYLFAGVVDGRNIW+NDL +S+ TL+ALE VVGK+K+VVSTSCSLLHTAVDLVNETKL
Sbjct: 348 SGKYLFAGVVDGRNIWANDLDSSLKTLQALEGVVGKDKVVVSTSCSLLHTAVDLVNETKL 407

Query: 406 DDEIKSWLAFAAQKVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAA 465
           D EIKSWLAFAAQKVVEVNALA+AL G KDE  FS+NAAA ASRKSSPRVTNEAVQKAAA
Sbjct: 408 DKEIKSWLAFAAQKVVEVNALAQALFGLKDETVFSANAAALASRKSSPRVTNEAVQKAAA 467

Query: 466 ALKGSDHRRSTNVSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDY 525
           ALKGSDHRR+TNVSARLDAQQKKLNLP+LPTTTIGSFPQTM+LR+VRRE+KAKKISE+DY
Sbjct: 468 ALKGSDHRRATNVSARLDAQQKKLNLPILPTTTIGSFPQTMELRKVRREYKAKKISEDDY 527

Query: 526 VSSIKEEINKVVQLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCV 585
           V +IKEEI+KVV+LQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCV
Sbjct: 528 VKAIKEEISKVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCV 587

Query: 586 KPPIIYGDVSRPKPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQI 645
           KPPIIYGDVSRPK MTVFWS+MAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQI
Sbjct: 588 KPPIIYGDVSRPKAMTVFWSSMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQI 647

Query: 646 ALAIKDEVEDLEKAGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQ 705
           ALAIKDEVEDLEKAG+TVIQIDEAALREGLPLRKSE+ FYL+WAVHSFRITNCGV+DTTQ
Sbjct: 648 ALAIKDEVEDLEKAGVTVIQIDEAALREGLPLRKSEQAFYLDWAVHSFRITNCGVKDTTQ 707

Query: 706 IHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSP 765
           IHTHMCYSNFNDIIHSII+MDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSP
Sbjct: 708 IHTHMCYSNFNDIIHSIINMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSP 767

Query: 766 RIPSTEEIADRINKMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLAS 825
           RIPSTEEIADRINKMLAVLESNILWVNPDCGLKTRKY+EVKPAL NMVAAAKLLRSQLAS
Sbjct: 768 RIPSTEEIADRINKMLAVLESNILWVNPDCGLKTRKYSEVKPALSNMVAAAKLLRSQLAS 827

Query: 826 AK 828
           AK
Sbjct: 828 AK 827

BLAST of Cucsa.142360 vs. TAIR10
Match: AT5G17920.1 (AT5G17920.1 Cobalamin-independent synthase family protein)

HSP 1 Score: 1355.5 bits (3507), Expect = 0.0e+00
Identity = 668/765 (87.32%), Postives = 720/765 (94.12%), Query Frame = 1

Query: 63  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 122
           MASHIVGYPR+GPKRELKFALESFWDGKS+A+DLQKV+ADLRSSIWKQM+ AG KFIPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSTAEDLQKVSADLRSSIWKQMSAAGTKFIPSN 60

Query: 123 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 182
           TF++YDQVLDT  MLGAVP RYG+ GGEIG D+YFSMARGNASVPAMEMTKWFDTNYHYI
Sbjct: 61  TFAHYDQVLDTTAMLGAVPPRYGYTGGEIGLDVYFSMARGNASVPAMEMTKWFDTNYHYI 120

Query: 183 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 242
           VPEL PE  F+YASHKAVNE+KEAKALGV+TVPVLVGPVS+LLLSK AKGV+KSF LLSL
Sbjct: 121 VPELGPEVNFSYASHKAVNEYKEAKALGVDTVPVLVGPVSYLLLSKAAKGVDKSFELLSL 180

Query: 243 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGLNVLI 302
           + KILP+YKEV++ELKAAGATWIQ DEP LV DL+   LQAFT AY+ELESTLSGLNVL+
Sbjct: 181 LPKILPIYKEVITELKAAGATWIQLDEPVLVMDLEGQKLQAFTGAYAELESTLSGLNVLV 240

Query: 303 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDGRNIWS 362
           ETYFAD+PAEAYKTLTSLKGV+ +GFDLVRGT+T+DL+K GFP GKYLFAGVVDGRNIW+
Sbjct: 241 ETYFADIPAEAYKTLTSLKGVTAFGFDLVRGTKTLDLVKAGFPEGKYLFAGVVDGRNIWA 300

Query: 363 NDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVE 422
           ND  AS++TL+ALE +VGK+K+VVSTSCSLLHTAVDL+NETKLDDEIKSWLAFAAQKVVE
Sbjct: 301 NDFAASLSTLQALEGIVGKDKLVVSTSCSLLHTAVDLINETKLDDEIKSWLAFAAQKVVE 360

Query: 423 VNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSARL 482
           VNALAKAL+G KDEA FS+NAAA ASR+SSPRVTNE VQKAAAALKGSDHRR+TNVSARL
Sbjct: 361 VNALAKALAGQKDEALFSANAAALASRRSSPRVTNEGVQKAAAALKGSDHRRATNVSARL 420

Query: 483 DAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQEE 542
           DAQQKKLNLP+LPTTTIGSFPQT++LRRVRRE+KAKK+SEEDYV +IKEEI KVV LQEE
Sbjct: 421 DAQQKKLNLPILPTTTIGSFPQTVELRRVRREYKAKKVSEEDYVKAIKEEIKKVVDLQEE 480

Query: 543 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTV 602
           LDIDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPP+IYGDVSRPK MTV
Sbjct: 481 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPVIYGDVSRPKAMTV 540

Query: 603 FWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIT 662
           FWS MAQSMT RPMKGMLTGPVTILNWSFVRNDQPR ETCYQIALAIKDEVEDLEK GI 
Sbjct: 541 FWSAMAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKGGIG 600

Query: 663 VIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSI 722
           VIQIDEAALREGLPLRKSE  FYL+WAVHSFRITNCGVQD+TQIHTHMCYS+FNDIIHSI
Sbjct: 601 VIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDSTQIHTHMCYSHFNDIIHSI 660

Query: 723 IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLA 782
           IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPS+EEIADR+NKMLA
Sbjct: 661 IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSSEEIADRVNKMLA 720

Query: 783 VLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 828
           VLE NILWVNPDCGLKTRKYTEVKPAL NMV AAKL+RSQLASAK
Sbjct: 721 VLEQNILWVNPDCGLKTRKYTEVKPALKNMVDAAKLIRSQLASAK 765

BLAST of Cucsa.142360 vs. TAIR10
Match: AT3G03780.1 (AT3G03780.1 methionine synthase 2)

HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 663/765 (86.67%), Postives = 720/765 (94.12%), Query Frame = 1

Query: 63  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 122
           MASHIVGYPR+GPKRELKFALESFWDGKSSADDLQKV+ADLRS IWKQM+ AGIK+IPSN
Sbjct: 1   MASHIVGYPRMGPKRELKFALESFWDGKSSADDLQKVSADLRSDIWKQMSAAGIKYIPSN 60

Query: 123 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 182
           TFS+YDQVLDT  MLGAVPSRYG+  GEIG D+YFSMARGNASVPAMEMTKWFDTNYHYI
Sbjct: 61  TFSHYDQVLDTTAMLGAVPSRYGFTSGEIGLDVYFSMARGNASVPAMEMTKWFDTNYHYI 120

Query: 183 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 242
           VPEL PE KF+YASHKAVNE+KEAKALGVETVPVLVGPVS+LLLSK AKGV+KSF LLSL
Sbjct: 121 VPELGPEVKFSYASHKAVNEYKEAKALGVETVPVLVGPVSYLLLSKLAKGVDKSFDLLSL 180

Query: 243 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGLNVLI 302
           + KILPVYKEV++ELKAAGA+WIQ DEP  V DL+ H LQAF+ AY+ELESTLSGLNVL+
Sbjct: 181 LPKILPVYKEVIAELKAAGASWIQLDEPLFVMDLEGHKLQAFSGAYAELESTLSGLNVLV 240

Query: 303 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDGRNIWS 362
           ETYFAD+PAEAYKTLTSLKGV+ +GFDLVRGT+T+DLIK GFP GKYLFAGVVDGRNIW+
Sbjct: 241 ETYFADIPAEAYKTLTSLKGVTAFGFDLVRGTKTIDLIKSGFPQGKYLFAGVVDGRNIWA 300

Query: 363 NDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVE 422
           NDL AS+ TL++LE VVGK+K+VVSTSCSLLHTAVDL+NETKLD EIKSWLAFAAQKVVE
Sbjct: 301 NDLAASLITLQSLEGVVGKDKLVVSTSCSLLHTAVDLINETKLDAEIKSWLAFAAQKVVE 360

Query: 423 VNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSARL 482
           V+ALAKAL+G  +E++F++NA A +SR+SSPRVTNE+VQKAAAALKGSDHRR+T VSARL
Sbjct: 361 VDALAKALAGQTNESFFTANADALSSRRSSPRVTNESVQKAAAALKGSDHRRTTEVSARL 420

Query: 483 DAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQEE 542
           DAQQKKLNLP+LPTTTIGSFPQT++LRRVRRE+KAKKISEEDYV +IKEEI KVV +QE+
Sbjct: 421 DAQQKKLNLPILPTTTIGSFPQTVELRRVRREYKAKKISEEDYVKAIKEEIKKVVDIQED 480

Query: 543 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTV 602
           LDIDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPP+IYGDVSRPKPMTV
Sbjct: 481 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPVIYGDVSRPKPMTV 540

Query: 603 FWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIT 662
           FWS+ AQSMTKRPMKGMLTGPVTILNWSFVRNDQPR ETCYQIALAIKDEVEDLEK GI 
Sbjct: 541 FWSSTAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKGGIG 600

Query: 663 VIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSI 722
           VIQIDEAALREGLPLRK+E +FYL+WAVHSFRITNCGVQD+TQIHTHMCYSNFNDIIHSI
Sbjct: 601 VIQIDEAALREGLPLRKAEHSFYLDWAVHSFRITNCGVQDSTQIHTHMCYSNFNDIIHSI 660

Query: 723 IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLA 782
           IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPST+EIADRINKMLA
Sbjct: 661 IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTDEIADRINKMLA 720

Query: 783 VLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 828
           VLE NILWVNPDCGLKTRKYTEVKPAL  MV AAKL+RSQL SAK
Sbjct: 721 VLEQNILWVNPDCGLKTRKYTEVKPALKAMVDAAKLIRSQLGSAK 765

BLAST of Cucsa.142360 vs. TAIR10
Match: AT5G20980.1 (AT5G20980.1 methionine synthase 3)

HSP 1 Score: 1287.7 bits (3331), Expect = 0.0e+00
Identity = 650/823 (78.98%), Postives = 714/823 (86.76%), Query Frame = 1

Query: 1   MTQLPLKSLEPFAISSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRSTSTVV 60
           M QL L+ L+P A              R  ++ P S    S+   LP    S R    V 
Sbjct: 1   MGQLALQRLQPLAS----------LPRRPPSLPPPS----SATPSLPCATASRRPRFYVA 60

Query: 61  RAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIP 120
           RAM+SHIVGYPRIGPKRELKFALESFWDGK++ DDLQ VAA+LR SIWK MA AGIK+IP
Sbjct: 61  RAMSSHIVGYPRIGPKRELKFALESFWDGKTNVDDLQNVAANLRKSIWKHMAHAGIKYIP 120

Query: 121 SNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYH 180
           SNTFSYYDQ+LDT  MLGAVPSRYGW  GEIGFD+YFSMARGNAS  AMEMTKWFDTNYH
Sbjct: 121 SNTFSYYDQMLDTTAMLGAVPSRYGWESGEIGFDVYFSMARGNASAHAMEMTKWFDTNYH 180

Query: 181 YIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLL 240
           YIVPEL P+  F+YASHKAV EFKEAKALG++TVPVL+GP+++LLLSKPAKGVEKSF LL
Sbjct: 181 YIVPELGPDVNFSYASHKAVVEFKEAKALGIDTVPVLIGPMTYLLLSKPAKGVEKSFCLL 240

Query: 241 SLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGLNV 300
           SLIDKILPVYKEV+++LK+AGA WIQFDEP LV DLD   LQAF+ AYS +ES+L+GLNV
Sbjct: 241 SLIDKILPVYKEVLADLKSAGARWIQFDEPILVMDLDTSQLQAFSDAYSHMESSLAGLNV 300

Query: 301 LIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDGRNI 360
           LI TYFADVPAEAYKTL SLK V+G+GFDLVRG +T+DLIK  FP GK LFAGVVDGRNI
Sbjct: 301 LIATYFADVPAEAYKTLMSLKCVTGFGFDLVRGLETLDLIKMNFPRGKLLFAGVVDGRNI 360

Query: 361 WSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKV 420
           W+NDL AS+ TL+ LE++VGKEK+VVSTSCSLLHTAVDLVNE KLD E+KSWLAFAAQKV
Sbjct: 361 WANDLSASLKTLQTLEDIVGKEKVVVSTSCSLLHTAVDLVNEMKLDKELKSWLAFAAQKV 420

Query: 421 VEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSA 480
           VEVNALAK+ SG KDEA FSSN+  QASR+SSPRVTN AVQ+   A+K SDH RST VS 
Sbjct: 421 VEVNALAKSFSGAKDEALFSSNSMRQASRRSSPRVTNAAVQQDVDAVKKSDHHRSTEVSV 480

Query: 481 RLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQ 540
           RL AQQKKLNLP LPTTTIGSFPQT DLRR+RREFKAKKISE DYV +IKEE  KV++LQ
Sbjct: 481 RLQAQQKKLNLPALPTTTIGSFPQTTDLRRIRREFKAKKISEVDYVQTIKEEYEKVIKLQ 540

Query: 541 EELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPM 600
           EEL IDVLVHGE ERNDMVE+FGEQLSGFAFT NGWVQSYGSRCVKPPIIYGD++RPK M
Sbjct: 541 EELGIDVLVHGEAERNDMVEFFGEQLSGFAFTSNGWVQSYGSRCVKPPIIYGDITRPKAM 600

Query: 601 TVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAG 660
           TVFWS+MAQ MT+RPMKGMLTGPVTILNWSFVRNDQPR ETC+QIALAIKDEVEDLEKAG
Sbjct: 601 TVFWSSMAQKMTQRPMKGMLTGPVTILNWSFVRNDQPRHETCFQIALAIKDEVEDLEKAG 660

Query: 661 ITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIH 720
           +TVIQIDEAALREGLPLRKSE+ FYL+WAVH+FRITN GVQD+TQIHTHMCYSNFNDIIH
Sbjct: 661 VTVIQIDEAALREGLPLRKSEQKFYLDWAVHAFRITNSGVQDSTQIHTHMCYSNFNDIIH 720

Query: 721 SIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKM 780
           SIIDMDADVITIENSRSDEKLLSVF EGVKYGAGIGPGVYDIHSPRIPSTEEIA+RINKM
Sbjct: 721 SIIDMDADVITIENSRSDEKLLSVFHEGVKYGAGIGPGVYDIHSPRIPSTEEIAERINKM 780

Query: 781 LAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQL 824
           LAVL+S +LWVNPDCGLKTR Y+EVK AL NMVAAAKL+RSQL
Sbjct: 781 LAVLDSKVLWVNPDCGLKTRNYSEVKSALSNMVAAAKLIRSQL 809

BLAST of Cucsa.142360 vs. NCBI nr
Match: gi|449452654|ref|XP_004144074.1| (PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [Cucumis sativus])

HSP 1 Score: 1629.0 bits (4217), Expect = 0.0e+00
Identity = 827/827 (100.00%), Postives = 827/827 (100.00%), Query Frame = 1

Query: 1   MTQLPLKSLEPFAISSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRSTSTVV 60
           MTQLPLKSLEPFAISSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRSTSTVV
Sbjct: 1   MTQLPLKSLEPFAISSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRSTSTVV 60

Query: 61  RAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIP 120
           RAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIP
Sbjct: 61  RAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIP 120

Query: 121 SNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYH 180
           SNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYH
Sbjct: 121 SNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYH 180

Query: 181 YIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLL 240
           YIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLL
Sbjct: 181 YIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLL 240

Query: 241 SLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGLNV 300
           SLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGLNV
Sbjct: 241 SLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGLNV 300

Query: 301 LIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDGRNI 360
           LIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDGRNI
Sbjct: 301 LIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDGRNI 360

Query: 361 WSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKV 420
           WSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKV
Sbjct: 361 WSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKV 420

Query: 421 VEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSA 480
           VEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSA
Sbjct: 421 VEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSA 480

Query: 481 RLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQ 540
           RLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQ
Sbjct: 481 RLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQ 540

Query: 541 EELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPM 600
           EELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPM
Sbjct: 541 EELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPM 600

Query: 601 TVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAG 660
           TVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAG
Sbjct: 601 TVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAG 660

Query: 661 ITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIH 720
           ITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIH
Sbjct: 661 ITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIH 720

Query: 721 SIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKM 780
           SIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKM
Sbjct: 721 SIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKM 780

Query: 781 LAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 828
           LAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK
Sbjct: 781 LAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 827

BLAST of Cucsa.142360 vs. NCBI nr
Match: gi|659100299|ref|XP_008451024.1| (PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [Cucumis melo])

HSP 1 Score: 1604.7 bits (4154), Expect = 0.0e+00
Identity = 817/829 (98.55%), Postives = 822/829 (99.16%), Query Frame = 1

Query: 1   MTQLPLKSLEPFAISSSSSSSFS--IFALRRGAIAPFSYLLFSSIHHLPSLRLSIRSTST 60
           MTQLPLKSLEPFAISSSSSSS S  IFALRRGAIAPFSYLLFSSIHHLPSLRLSIR+TST
Sbjct: 1   MTQLPLKSLEPFAISSSSSSSSSSSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRTTST 60

Query: 61  VVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKF 120
           VVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMA+AGIKF
Sbjct: 61  VVRAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMADAGIKF 120

Query: 121 IPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTN 180
           IPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTN
Sbjct: 121 IPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTN 180

Query: 181 YHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFS 240
           YHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFS
Sbjct: 181 YHYIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFS 240

Query: 241 LLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGL 300
           LLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAH LQAF  AYSELESTLSGL
Sbjct: 241 LLSLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHQLQAFAHAYSELESTLSGL 300

Query: 301 NVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDGR 360
           NVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQT+DLIKGGFPSGKYLFAGVVDGR
Sbjct: 301 NVLIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTLDLIKGGFPSGKYLFAGVVDGR 360

Query: 361 NIWSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQ 420
           NIWSN+LQASITTLEAL NVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQ
Sbjct: 361 NIWSNNLQASITTLEALGNVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQ 420

Query: 421 KVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNV 480
           KVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRR+TNV
Sbjct: 421 KVVEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNV 480

Query: 481 SARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQ 540
           SARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQ
Sbjct: 481 SARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQ 540

Query: 541 LQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPK 600
           LQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPK
Sbjct: 541 LQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPK 600

Query: 601 PMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEK 660
           PMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEK
Sbjct: 601 PMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEK 660

Query: 661 AGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDI 720
           AGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDI
Sbjct: 661 AGITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDI 720

Query: 721 IHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRIN 780
           IHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRIN
Sbjct: 721 IHSIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRIN 780

Query: 781 KMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 828
           KMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK
Sbjct: 781 KMLAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 829

BLAST of Cucsa.142360 vs. NCBI nr
Match: gi|1009113717|ref|XP_015873297.1| (PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [Ziziphus jujuba])

HSP 1 Score: 1416.7 bits (3666), Expect = 0.0e+00
Identity = 717/827 (86.70%), Postives = 759/827 (91.78%), Query Frame = 1

Query: 1   MTQLPLKSLEPFAISSSSSSSFSIFALRRGAIAPFSYLLFSSIHHLPSLRLSIRSTSTVV 60
           M Q  L SL P   S   S +F   +L                  L S  LS    S   
Sbjct: 1   MPQASLNSLAPLRSSLRISITFGFASLP-----------------LSSNCLSFLRFSFPT 60

Query: 61  RAMASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIP 120
           RAMASHIVGYPR+GPKRELKFALESFWDGKSSADDLQKV+ADLRSSIWKQMA+AGIK+IP
Sbjct: 61  RAMASHIVGYPRMGPKRELKFALESFWDGKSSADDLQKVSADLRSSIWKQMADAGIKYIP 120

Query: 121 SNTFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYH 180
           SNTFSYYDQVLDT  MLGAVP RYGWNGGEIGFD++FSMARGNASVPAMEMTKWFDTNYH
Sbjct: 121 SNTFSYYDQVLDTTAMLGAVPPRYGWNGGEIGFDVFFSMARGNASVPAMEMTKWFDTNYH 180

Query: 181 YIVPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLL 240
           YIVPEL PE KF+YASHKAVNEFKEAK+LG++TVPV+VGPVS+LLLSKPAKGVEKSFSLL
Sbjct: 181 YIVPELGPEVKFSYASHKAVNEFKEAKSLGIDTVPVIVGPVSYLLLSKPAKGVEKSFSLL 240

Query: 241 SLIDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGLNV 300
           SL+  ILPVYKEVV+ELKAAGATWIQFDEPTLVKDLD+H LQAFT AYSELES+LSGLNV
Sbjct: 241 SLLGNILPVYKEVVAELKAAGATWIQFDEPTLVKDLDSHQLQAFTHAYSELESSLSGLNV 300

Query: 301 LIETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDGRNI 360
           LIETYFADVPAEA+KTLTSLKGV+GYGFDLVRGT+T+DLIKGGFP GKYLFAGVVDGRNI
Sbjct: 301 LIETYFADVPAEAFKTLTSLKGVTGYGFDLVRGTKTLDLIKGGFPFGKYLFAGVVDGRNI 360

Query: 361 WSNDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKV 420
           W+NDL +S+ TL ALE +VGK+K+VVSTSCSLLHTAVDLVNETKLD EIKSWLAFAAQKV
Sbjct: 361 WANDLASSLDTLSALEAIVGKDKLVVSTSCSLLHTAVDLVNETKLDKEIKSWLAFAAQKV 420

Query: 421 VEVNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSA 480
           VEVNALAKAL+G +DEA+FSSNA AQASRKSSPRVTNEAVQKAAAALKGSDHRR+TNVSA
Sbjct: 421 VEVNALAKALAGQRDEAFFSSNATAQASRKSSPRVTNEAVQKAAAALKGSDHRRATNVSA 480

Query: 481 RLDAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQ 540
           RLDAQQK+LNLP+LPTTTIGSFPQTMDLRRVRREFKAKKISEE+YV +I EEI+KVV+LQ
Sbjct: 481 RLDAQQKQLNLPILPTTTIGSFPQTMDLRRVRREFKAKKISEEEYVKAITEEISKVVKLQ 540

Query: 541 EELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPM 600
           EELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPK M
Sbjct: 541 EELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKAM 600

Query: 601 TVFWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAG 660
           TVFWS++AQSMTKRPMKGMLTGPVTILNWSFVRNDQPR ETCYQIALAIKDEVEDLEKAG
Sbjct: 601 TVFWSSLAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRSETCYQIALAIKDEVEDLEKAG 660

Query: 661 ITVIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIH 720
           +TVIQIDEAALREGLPLRKSEE FYLNWAVHSFRITNCGV+DTTQIHTHMCYSNFNDIIH
Sbjct: 661 VTVIQIDEAALREGLPLRKSEEAFYLNWAVHSFRITNCGVKDTTQIHTHMCYSNFNDIIH 720

Query: 721 SIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKM 780
           SIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKM
Sbjct: 721 SIIDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKM 780

Query: 781 LAVLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 828
           LAVLESNILWVNPDCGLKTRKYTEVKPAL NMVAAAKLLR QL SAK
Sbjct: 781 LAVLESNILWVNPDCGLKTRKYTEVKPALSNMVAAAKLLRQQLGSAK 810

BLAST of Cucsa.142360 vs. NCBI nr
Match: gi|703104128|ref|XP_010097930.1| (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [Morus notabilis])

HSP 1 Score: 1413.3 bits (3657), Expect = 0.0e+00
Identity = 701/765 (91.63%), Postives = 742/765 (96.99%), Query Frame = 1

Query: 63  MASHIVGYPRIGPKRELKFALESFWDGKSSADDLQKVAADLRSSIWKQMAEAGIKFIPSN 122
           MASHIVGYPR+GPKRELKFALESFWDGKSSA+DLQKVAADLR+SIWKQM+EAGIK+IPSN
Sbjct: 1   MASHIVGYPRVGPKRELKFALESFWDGKSSAEDLQKVAADLRASIWKQMSEAGIKYIPSN 60

Query: 123 TFSYYDQVLDTATMLGAVPSRYGWNGGEIGFDIYFSMARGNASVPAMEMTKWFDTNYHYI 182
           TFSYYDQVLDT  MLGAVP RYGWNGGEIGFD+YFSMARGNASVPAMEMTKWFDTNYHYI
Sbjct: 61  TFSYYDQVLDTTAMLGAVPPRYGWNGGEIGFDVYFSMARGNASVPAMEMTKWFDTNYHYI 120

Query: 183 VPELSPEDKFTYASHKAVNEFKEAKALGVETVPVLVGPVSFLLLSKPAKGVEKSFSLLSL 242
           VPELSPE KF+YASHKAV E+KEAKALGVETVPVLVGPVS+LLLSKPAKGVEKSFSLLSL
Sbjct: 121 VPELSPEIKFSYASHKAVEEYKEAKALGVETVPVLVGPVSYLLLSKPAKGVEKSFSLLSL 180

Query: 243 IDKILPVYKEVVSELKAAGATWIQFDEPTLVKDLDAHHLQAFTQAYSELESTLSGLNVLI 302
           I  ILPVYKEV++ELKAAGATW+QFDEPTLVKDLDAH LQAFT AYSELES+LSGLNV+I
Sbjct: 181 IGSILPVYKEVLAELKAAGATWVQFDEPTLVKDLDAHQLQAFTHAYSELESSLSGLNVVI 240

Query: 303 ETYFADVPAEAYKTLTSLKGVSGYGFDLVRGTQTVDLIKGGFPSGKYLFAGVVDGRNIWS 362
           ETYFADV AEA+KTLT LKGV+GYGFDLVRGT+T+DLIKGGFPSGKYLFAGVVDGRNIW+
Sbjct: 241 ETYFADVTAEAFKTLTGLKGVTGYGFDLVRGTKTLDLIKGGFPSGKYLFAGVVDGRNIWA 300

Query: 363 NDLQASITTLEALENVVGKEKIVVSTSCSLLHTAVDLVNETKLDDEIKSWLAFAAQKVVE 422
           NDL +S++TLEALE +VGK+K+VVSTSCSLLHTAVDLVNETKLD EIKSWLAFAAQKVVE
Sbjct: 301 NDLASSLSTLEALEGIVGKDKLVVSTSCSLLHTAVDLVNETKLDKEIKSWLAFAAQKVVE 360

Query: 423 VNALAKALSGHKDEAYFSSNAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRSTNVSARL 482
           VNALAKAL+G KDEA+F++NA AQASR+SSPRVTNEAVQKAAAALKGSDHRR+TNVS+RL
Sbjct: 361 VNALAKALAGQKDEAFFTANAGAQASRRSSPRVTNEAVQKAAAALKGSDHRRATNVSSRL 420

Query: 483 DAQQKKLNLPMLPTTTIGSFPQTMDLRRVRREFKAKKISEEDYVSSIKEEINKVVQLQEE 542
           DAQQKKLNLP LPTTTIGSFPQT++LRRVRRE+KAKKISEE+YV++IKEEI KVV+LQEE
Sbjct: 421 DAQQKKLNLPALPTTTIGSFPQTLELRRVRREYKAKKISEEEYVNAIKEEIKKVVKLQEE 480

Query: 543 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTV 602
           LDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTV
Sbjct: 481 LDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSRPKPMTV 540

Query: 603 FWSTMAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIT 662
           FWS+ AQS TKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIT
Sbjct: 541 FWSSFAQSTTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGIT 600

Query: 663 VIQIDEAALREGLPLRKSEENFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSI 722
           VIQIDEAALREGLPLRKSEE FYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSI
Sbjct: 601 VIQIDEAALREGLPLRKSEEAFYLNWAVHSFRITNCGVQDTTQIHTHMCYSNFNDIIHSI 660

Query: 723 IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSTEEIADRINKMLA 782
           IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPS EEIADRINKMLA
Sbjct: 661 IDMDADVITIENSRSDEKLLSVFREGVKYGAGIGPGVYDIHSPRIPSREEIADRINKMLA 720

Query: 783 VLESNILWVNPDCGLKTRKYTEVKPALLNMVAAAKLLRSQLASAK 828
           VLESNILWVNPDCGLKTRKY+EVKPAL NMVAAAKLLRSQLASAK
Sbjct: 721 VLESNILWVNPDCGLKTRKYSEVKPALSNMVAAAKLLRSQLASAK 765

BLAST of Cucsa.142360 vs. NCBI nr
Match: gi|356547867|ref|XP_003542326.1| (PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 1 [Glycine max])

HSP 1 Score: 1404.0 bits (3633), Expect = 0.0e+00
Identity = 700/796 (87.94%), Postives = 750/796 (94.22%), Query Frame = 1

Query: 32  IAPFSYLLFSSIHHLPSLRLSIRSTSTVVRAMASHIVGYPRIGPKRELKFALESFWDGKS 91
           +APF++ L +      SLR S+R+TS+  RAMASHIVGYPR+GPKRELKFALESFWDGKS
Sbjct: 10  VAPFTFSLCTLSFSSYSLRFSVRATSS--RAMASHIVGYPRMGPKRELKFALESFWDGKS 69

Query: 92  SADDLQKVAADLRSSIWKQMAEAGIKFIPSNTFSYYDQVLDTATMLGAVPSRYGWNGGEI 151
           SA++LQKVAADLRS+IWKQMA+AGIK+IPSNTFS YDQVLDT  MLGAVPSRY WNGGEI
Sbjct: 70  SAEELQKVAADLRSAIWKQMADAGIKYIPSNTFSLYDQVLDTTAMLGAVPSRYNWNGGEI 129

Query: 152 GFDIYFSMARGNASVPAMEMTKWFDTNYHYIVPELSPEDKFTYASHKAVNEFKEAKALGV 211
           GFD+YFSMARGNASVPAMEMTKWFDTNYHYIVPEL P+ KF+YASHKAV+EFKEAK LGV
Sbjct: 130 GFDVYFSMARGNASVPAMEMTKWFDTNYHYIVPELGPDVKFSYASHKAVDEFKEAKVLGV 189

Query: 212 ETVPVLVGPVSFLLLSKPAKGVEKSFSLLSLIDKILPVYKEVVSELKAAGATWIQFDEPT 271
            TVPVLVGPVS+LLLSKPAKGVEKSFSLLSLIDKILPVY+EVV+ELKAAGATWIQFDEPT
Sbjct: 190 NTVPVLVGPVSYLLLSKPAKGVEKSFSLLSLIDKILPVYREVVAELKAAGATWIQFDEPT 249

Query: 272 LVKDLDAHHLQAFTQAYSELESTLSGLNVLIETYFADVPAEAYKTLTSLKGVSGYGFDLV 331
           LVKDL+ H LQAFT AY+ELES+LSG NVLIETYFADVPAEAYKTLTSLK V+ YGFD+V
Sbjct: 250 LVKDLNTHQLQAFTHAYAELESSLSGFNVLIETYFADVPAEAYKTLTSLKAVTAYGFDIV 309

Query: 332 RGTQTVDLIKGGFPSGKYLFAGVVDGRNIWSNDLQASITTLEALENVVGKEKIVVSTSCS 391
           RGT+T+DLIK GFPSGK+LFAGVVDGRNIW+N L +S+ TL+AL ++VG +K+VVSTSCS
Sbjct: 310 RGTKTLDLIKQGFPSGKFLFAGVVDGRNIWANSLASSLNTLQALGDIVGNDKVVVSTSCS 369

Query: 392 LLHTAVDLVNETKLDDEIKSWLAFAAQKVVEVNALAKALSGHKDEAYFSSNAAAQASRKS 451
           LLHTAVDLVNETKLD EIKSWLAFAAQKVVEVNALAKALSG KDE +FS+NAAA  SRKS
Sbjct: 370 LLHTAVDLVNETKLDQEIKSWLAFAAQKVVEVNALAKALSGQKDEVFFSANAAALDSRKS 429

Query: 452 SPRVTNEAVQKAAAALKGSDHRRSTNVSARLDAQQKKLNLPMLPTTTIGSFPQTMDLRRV 511
           SPRVTNEAVQKAAAALKGSDHRR+TNVSARLD+QQKKLNLP+LPTTTIGSFPQT DLRRV
Sbjct: 430 SPRVTNEAVQKAAAALKGSDHRRATNVSARLDSQQKKLNLPVLPTTTIGSFPQTADLRRV 489

Query: 512 RREFKAKKISEEDYVSSIKEEINKVVQLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAF 571
           RREFKA KISEEDY+  IKEEIN VV+LQEELDIDVLVHGEPERNDMVEYFGEQLSGFAF
Sbjct: 490 RREFKANKISEEDYIHFIKEEINNVVKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAF 549

Query: 572 TVNGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWSTMAQSMTKRPMKGMLTGPVTILNWSF 631
           T NGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWS+ AQS+TKRPMKGMLTGPVTILNWSF
Sbjct: 550 TANGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWSSTAQSLTKRPMKGMLTGPVTILNWSF 609

Query: 632 VRNDQPRFETCYQIALAIKDEVEDLEKAGITVIQIDEAALREGLPLRKSEENFYLNWAVH 691
           VR+DQPRFETCYQIALAIKDEVEDLEKAGITVIQIDEAALREGLPLRKSEE FYLNWAVH
Sbjct: 610 VRDDQPRFETCYQIALAIKDEVEDLEKAGITVIQIDEAALREGLPLRKSEEAFYLNWAVH 669

Query: 692 SFRITNCGVQDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKY 751
           SFRITNCGV+DTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKY
Sbjct: 670 SFRITNCGVEDTTQIHTHMCYSNFNDIIHSIIDMDADVITIENSRSDEKLLSVFREGVKY 729

Query: 752 GAGIGPGVYDIHSPRIPSTEEIADRINKMLAVLESNILWVNPDCGLKTRKYTEVKPALLN 811
           GAGIGPG+YDIHSPRIP TEEIADRINKMLAVLESNILWVNPDCGLKTRKYTEVKPAL N
Sbjct: 730 GAGIGPGIYDIHSPRIPPTEEIADRINKMLAVLESNILWVNPDCGLKTRKYTEVKPALTN 789

Query: 812 MVAAAKLLRSQLASAK 828
           MVAAAKL+R+QLASAK
Sbjct: 790 MVAAAKLIRNQLASAK 803