Cucsa.100510 (gene) Cucumber (Gy14) v1

NameCucsa.100510
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionThreonine--tRNA ligase
Locationscaffold00926 : 951470 .. 956020 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAGACTCATATCTTCTCCGGCATCTGCTCGCTCTTTCTTTCTTCAATGTTTGTTCTTCAAAGAATGGCGACTACCACTGGCTTACTCTTACACTCTCCTCTCGTAACACCTATTTCCCCTAACGTCTCTGCTCCTCGCTTCTTCCTTGCAGTTTCGACCAAATTTGCAAGAAATAGGGACAGACCTTTTCTCCCTCGCAACAAGTCCAATGGCTTTTCCACTTCCATTTCTGTGGCTACTGATTCCTCAGCACCATCAATTTCGACCCAGAACGAGGACTTGGTGGATGCCCACAAGGATCAAACCGAGAAGATTGTTCTTCCAACTAATGAATCCTCTGAGAAGCTTCTCAGGATTCGTCACACGGTGACTCTGTTTTTtCTTTGATTTTTCCCTGTGGGTTTTGGGATAGATGTTATTTGAATGTGGGTATGGGTTCTTATATGAGAATATGAGGACTTTTTATGCTTAATGAAGGATTTTCTTAATGGAGTTCCCCTGGGTGAAGTTTAGGTTTGAACTAGGCAGGAGTTTCATTACTTAAACAGGGTGTTGCTAACCTAGAAGGGGTTTTCTGAATTCCATTTTGCATCTTGGAGATAAGATTGAATGCGGACTTTAGGTTTCAACTGCTTGATATATGCTTCTCGAATTGCAGTGTGCTCATGTAATGGCCATGGCTGTTCAGAAGCTCTTTCCAGATGCTAAAGTGACAATTGGACCATGGATTGAAAATGGGTTTTACTATGATTTTGACATGGAGCCCTTGGGAGATAAAGATTTGAAGAAAATCAAGAAGGAGATGGTTGGTATATACCAAATCAATGAGCATTGAAGTCACTGGTTTTTTTTCTTTATTATTTTACATCACATGATTTGGAATATATATATTAGTCAATCCTTTTACGGTGTGGGGGTTTATTTCTCACATCTTCTCTAGGAACGCATTATTAGCAGAAACTTACCACTTGTAAGGGAAGAAGTTTCAAGGGACGAAGCTCAAAGAAGAATTGAGGCAATCAACGAACCTTACAAGATGGAGCTTTTGGAAAGCATAAAGGAGGATCCCATAACCATCTATCATATTGGTAGGGATTGCAATCATCTTTGGTACTTATCTGTTCCACCTTTTGAGATCTTCTTGTATTTCATGCTTAGCAATGTTTGATACTTTTCTTGATTGAACACTAGATTCTTTTATCTTCTTTGTAATTGAAATTTAGCTTTGGCGTATGAAGGGCTTGAGTTTAGAGTACTCTTTTTCTTTCTGTTTCCCGGATTGATATAGATTGGGTCTTACAAGCAAAATATTGAAAAGAAAATACCCCAACGAAACTTAGTATTCATCAGAAGATTTTATTCGGCTATACACCATAAGAAGTAAGAGCAATGTTATGATGGTTAATTAATATTGGAGATGACTTGAAAACTGCTAAAAAaGATATTGACTAAATATTTTTGGCGGGTAAAGGATATATTTATTGTCCTTTATTTAACAGAGCTCTCACATGGGCTGTTGAAGTAAAAAGTGCTTATGAATTGAACAAACGTATAAAGTGTTCTTGTAAACACCTGAATATATTTTCTTAATGTGCTTGTAATTTATCTTCCTTTTTTTCTTTTGTGGAGAGATTCAAGGCTGACAAAGATGCAGTTTAGGAAATTTTCTTGTTTATATTCATGATCCTGAATGATTTTCAACAATTGAAAAGGCATTCCAATTAGGTCATTATTTAAAAACTGAAATTGTTCTTTTTCTTTGTTATGATCGTTGATTAGTATCACTTCGCTGTTAGGATTTTTAATGAACAGAAAGTGCAGAGAGAATCTCCCATACTTGAAATTAGCAAATTTCTCTCTTTTCTCCATACTCTTCCACAAAATGTTCTTTTGATTGCAATTAAAAGGATATTGGTAGCTGCAAAAGATGGCAAATGGTAAATCAATTGTCTAGTAAATTAAGAACATCTTGGTCTATTTTGATCAGTTGGTAATTCTATCCCGTAACCTTAATTGATGATTAAATTTGTAATGCTTTCTTAAGACTTCAATTGGTTCAGGGTGAAGTTGAAATTTTCCTATTATCTTGATCCGTAGTAGTATCCTATATTCTTCACAGGAAATGAATGGTGGGATCTTTGTGCTGGCCCCCATGTTGAATCTACTGGGAAAATCAACAAAAGAGCTGTTGAACTTGAAACAGTCGCCGGTGCATACTGGAGAGGAGACGAGAAGAATCCCATGCTACAGAGGATATACGGTACTGCTTGGGAGACAGAAGAACAATTAAAAGCATACCTTCACTTCAAAGAGGAAGCCAAACGTCGTGATCACAGACGGCTTGGGAAAGATCTTGATCTATTTTCCATTCAGGTGTATACTTCCTCCAAAACTATTATAATGTACTTGATTGTAGCGAGAGTTATGCTCTTAGATTTTGTTCATCTGAATTATTTTAGAGCATTGGCTGAACACAGGATGATGCTGGGGGAGGTTTGGTATTCTGGCACCCCAAGGGTGCTGTTGTGAGGAGCATAATAGAAGATTTATGGAAGAAATTGCACAGGGAACGTGGTTATGATATGCTATATACTCCACATGTGGCAAAAGCAGAGCTTTGGAAAACCAGTGGTCATTCAGATTTCTATGGAGAAAATATGTACAATCAGATGGACGTTGAGAATGAGCTTTATCAACTTCGACCTATGAATTGCCCCTACCATATTCTATTTTACAAAAGAAGGCTTACTTCCTATCAAGATTTTCCTATAAGAGTTGCAGAATTGGGGACGGTATATAGATATGAGCTGTCCGGTAGCTTGCGTGGTCTTTTCCGTGTCAGAGGTTTTACCCAGGTGCACTTTATGTTGGATGCTGTCATTTTCTTTATCATTTAAACTCTACAAGTTGTTATCTGCTCTATGCAGAAAATCGAAATTTCCAAGCTGTGAAGGATTGAAGTTATATTTTGATTGTTTCTCGTTTGCGGCTTAAATAATAAAGATTTTTTGCTGCTTTACTTACATGTTTTATGGGTTTTGCAGGATGATGCTCACATATTTTGTTTGGATGATCAAATCAAAGATGAAATTAGAGGTGTCCTAGATCTAACAGAAGATTTATTACTTCAATTTGGTTTTAACGAATTTGAAGTCAATCTCTCTACTAGACCACCAAAATCAGTGGGGAATGATGAAATCTGGGAGAAAGCAACATCTGCTCTTAAAGATGCTCTAGGTGACAAGGGGTGGGGCTATCAAATTGATGAAGGTGGTGGAGCCTTTTACGGACCAAAAATTGATATTAAGATCGAGGATGCATTGGGAAGGAAGTGGCAATGCTCAACTATACAGGCAATCCCTCAACTTAATATGGGTTTTTATTGGTAGATCTAATCTTTAGTGAAATCTCTTTCACTGTCCTGAAATCTTATGATTTCTACAAACTGAAGTTCATGGCAGTTTCTTTTGATGTATGAAAAAGCTATCTGACTTGAACTTGACTGTGGTTAAGTAAAGCTGTTCTTTCTTTAATATTTATTTATTTTATTTCTTTCAGGTTGATTTCAATCTACCTCAGCGTTTTGACATCACATATGTTGATTCAAACTCAGAAAAGAAGAGACCTATCATGATCCATCGTGCAGTGCTAGGATCCCTTGAGCGATTCTTCGGTATTCTAATAGAAAATTATGCTGGGGACTTCCCATTATGGCTTTCTCCAATCCAAGTTCGAGTTTTGCCCGTAACGGATGCCCAGGTATTATATATAAGCATAAAAATTCCAGCAGTTTGTGACATATTAGTTACGTTGTCACAATAATTTGCATGGTCCTGTCTTGATTATGTATGAAAGCTTATTGCCAACAAACCCATGCAAGATATTCATCAAGTTCTTAGGTAGAATCACGTCCCTGATGACGAGGTTCATTATACTTGGAATGGGATCGTGTAAATTTGATTTGCTAACTGAATCAATTGGAGAGATTCATTTTAGGTGTTAAAGTAGTGATATTCACTGAAATCTCCACTGCTTTTCTATCCCAGCTCTCATACTGCAAAGAGGTGAGCGAAAAACTCAGAGCCAGTGGAGTTCGAGCAGAAGTTTGTCACGGTGAGCGGTTGCCTAAGCTTATTAGAAATGCAGAGACCCAAAAAATACCATTGATGGCAGTTGTTGGTGCCAAGGAAGTCGAGATGCAAACTGTGACAGTGAGATCAAGGTTTGGTGGGGAGATAGGGACCATAGCAGTTGATGACTTTGTTGGAAGAATCAAATTCGCCTCAGAGAATAGAACTCCATTTTGAAGCGTTCTTTTCTAGGATGAACCACGGCCATTAAGAATTTTCCTTATTATTTGACTTGAAATTCCTCGTGTAGGTTAATTCCCTCAATGAACATATTCGCATTGTACTTTTTAGTATTCTTTCACTCCACATTTTGGCTGTAAATGTCTGTAATCTTGTAGTTACAGAAGACTATTTGATTTTGAGGATTTTCCATCCTCTAACGTGAATAATGAAAATATATATATATATATACTTAGAGCC

mRNA sequence

GCAGACTCATATCTTCTCCGGCATCTGCTCGCTCTTTCTTTCTTCAATGTTTGTTCTTCAAAGAATGGCGACTACCACTGGCTTACTCTTACACTCTCCTCTCGTAACACCTATTTCCCCTAACGTCTCTGCTCCTCGCTTCTTCCTTGCAGTTTCGACCAAATTTGCAAGAAATAGGGACAGACCTTTTCTCCCTCGCAACAAGTCCAATGGCTTTTCCACTTCCATTTCTGTGGCTACTGATTCCTCAGCACCATCAATTTCGACCCAGAACGAGGACTTGGTGGATGCCCACAAGGATCAAACCGAGAAGATTGTTCTTCCAACTAATGAATCCTCTGAGAAGCTTCTCAGGATTCGTCACACGTGTGCTCATGTAATGGCCATGGCTGTTCAGAAGCTCTTTCCAGATGCTAAAGTGACAATTGGACCATGGATTGAAAATGGGTTTTACTATGATTTTGACATGGAGCCCTTGGGAGATAAAGATTTGAAGAAAATCAAGAAGGAGATGGAACGCATTATTAGCAGAAACTTACCACTTGTAAGGGAAGAAGTTTCAAGGGACGAAGCTCAAAGAAGAATTGAGGCAATCAACGAACCTTACAAGATGGAGCTTTTGGAAAGCATAAAGGAGGATCCCATAACCATCTATCATATTGGAAATGAATGGTGGGATCTTTGTGCTGGCCCCCATGTTGAATCTACTGGGAAAATCAACAAAAGAGCTGTTGAACTTGAAACAGTCGCCGGTGCATACTGGAGAGGAGACGAGAAGAATCCCATGCTACAGAGGATATACGGTACTGCTTGGGAGACAGAAGAACAATTAAAAGCATACCTTCACTTCAAAGAGGAAGCCAAACGTCGTGATCACAGACGGCTTGGGAAAGATCTTGATCTATTTTCCATTCAGGATGATGCTGGGGGAGGTTTGGTATTCTGGCACCCCAAGGGTGCTGTTGTGAGGAGCATAATAGAAGATTTATGGAAGAAATTGCACAGGGAACGTGGTTATGATATGCTATATACTCCACATGTGGCAAAAGCAGAGCTTTGGAAAACCAGTGGTCATTCAGATTTCTATGGAGAAAATATGTACAATCAGATGGACGTTGAGAATGAGCTTTATCAACTTCGACCTATGAATTGCCCCTACCATATTCTATTTTACAAAAGAAGGCTTACTTCCTATCAAGATTTTCCTATAAGAGTTGCAGAATTGGGGACGGTATATAGATATGAGCTGTCCGGTAGCTTGCGTGGTCTTTTCCGTGTCAGAGGTTTTACCCAGGATGATGCTCACATATTTTGTTTGGATGATCAAATCAAAGATGAAATTAGAGGTGTCCTAGATCTAACAGAAGATTTATTACTTCAATTTGGTTTTAACGAATTTGAAGTCAATCTCTCTACTAGACCACCAAAATCAGTGGGGAATGATGAAATCTGGGAGAAAGCAACATCTGCTCTTAAAGATGCTCTAGGTGACAAGGGGTGGGGCTATCAAATTGATGAAGGTGGTGGAGCCTTTTACGGACCAAAAATTGATATTAAGATCGAGGATGCATTGGGAAGGAAGTGGCAATGCTCAACTATACAGGTTGATTTCAATCTACCTCAGCGTTTTGACATCACATATGTTGATTCAAACTCAGAAAAGAAGAGACCTATCATGATCCATCGTGCAGTGCTAGGATCCCTTGAGCGATTCTTCGGTATTCTAATAGAAAATTATGCTGGGGACTTCCCATTATGGCTTTCTCCAATCCAAGTTCGAGTTTTGCCCGTAACGGATGCCCAGCTCTCATACTGCAAAGAGGTGAGCGAAAAACTCAGAGCCAGTGGAGTTCGAGCAGAAGTTTGTCACGGTGAGCGGTTGCCTAAGCTTATTAGAAATGCAGAGACCCAAAAAATACCATTGATGGCAGTTGTTGGTGCCAAGGAAGTCGAGATGCAAACTGTGACAGTGAGATCAAGGTTTGGTGGGGAGATAGGGACCATAGCAGTTGATGACTTTGTTGGAAGAATCAAATTCGCCTCAGAGAATAGAACTCCATTTTGAAGCGTTCTTTTCTAGGATGAACCACGGCCATTAAGAATTTTCCTTATTATTTGACTTGAAATTCCTCGTGTAGGTTAATTCCCTCAATGAACATATTCGCATTGTACTTTTTAGTATTCTTTCACTCCACATTTTGGCTGTAAATGTCTGTAATCTTGTAGTTACAGAAGACTATTTGATTTTGAGGATTTTCCATCCTCTAACGTGAATAATGAAAATATATATATATATATACTTAGAGCC

Coding sequence (CDS)

ATGTTTGTTCTTCAAAGAATGGCGACTACCACTGGCTTACTCTTACACTCTCCTCTCGTAACACCTATTTCCCCTAACGTCTCTGCTCCTCGCTTCTTCCTTGCAGTTTCGACCAAATTTGCAAGAAATAGGGACAGACCTTTTCTCCCTCGCAACAAGTCCAATGGCTTTTCCACTTCCATTTCTGTGGCTACTGATTCCTCAGCACCATCAATTTCGACCCAGAACGAGGACTTGGTGGATGCCCACAAGGATCAAACCGAGAAGATTGTTCTTCCAACTAATGAATCCTCTGAGAAGCTTCTCAGGATTCGTCACACGTGTGCTCATGTAATGGCCATGGCTGTTCAGAAGCTCTTTCCAGATGCTAAAGTGACAATTGGACCATGGATTGAAAATGGGTTTTACTATGATTTTGACATGGAGCCCTTGGGAGATAAAGATTTGAAGAAAATCAAGAAGGAGATGGAACGCATTATTAGCAGAAACTTACCACTTGTAAGGGAAGAAGTTTCAAGGGACGAAGCTCAAAGAAGAATTGAGGCAATCAACGAACCTTACAAGATGGAGCTTTTGGAAAGCATAAAGGAGGATCCCATAACCATCTATCATATTGGAAATGAATGGTGGGATCTTTGTGCTGGCCCCCATGTTGAATCTACTGGGAAAATCAACAAAAGAGCTGTTGAACTTGAAACAGTCGCCGGTGCATACTGGAGAGGAGACGAGAAGAATCCCATGCTACAGAGGATATACGGTACTGCTTGGGAGACAGAAGAACAATTAAAAGCATACCTTCACTTCAAAGAGGAAGCCAAACGTCGTGATCACAGACGGCTTGGGAAAGATCTTGATCTATTTTCCATTCAGGATGATGCTGGGGGAGGTTTGGTATTCTGGCACCCCAAGGGTGCTGTTGTGAGGAGCATAATAGAAGATTTATGGAAGAAATTGCACAGGGAACGTGGTTATGATATGCTATATACTCCACATGTGGCAAAAGCAGAGCTTTGGAAAACCAGTGGTCATTCAGATTTCTATGGAGAAAATATGTACAATCAGATGGACGTTGAGAATGAGCTTTATCAACTTCGACCTATGAATTGCCCCTACCATATTCTATTTTACAAAAGAAGGCTTACTTCCTATCAAGATTTTCCTATAAGAGTTGCAGAATTGGGGACGGTATATAGATATGAGCTGTCCGGTAGCTTGCGTGGTCTTTTCCGTGTCAGAGGTTTTACCCAGGATGATGCTCACATATTTTGTTTGGATGATCAAATCAAAGATGAAATTAGAGGTGTCCTAGATCTAACAGAAGATTTATTACTTCAATTTGGTTTTAACGAATTTGAAGTCAATCTCTCTACTAGACCACCAAAATCAGTGGGGAATGATGAAATCTGGGAGAAAGCAACATCTGCTCTTAAAGATGCTCTAGGTGACAAGGGGTGGGGCTATCAAATTGATGAAGGTGGTGGAGCCTTTTACGGACCAAAAATTGATATTAAGATCGAGGATGCATTGGGAAGGAAGTGGCAATGCTCAACTATACAGGTTGATTTCAATCTACCTCAGCGTTTTGACATCACATATGTTGATTCAAACTCAGAAAAGAAGAGACCTATCATGATCCATCGTGCAGTGCTAGGATCCCTTGAGCGATTCTTCGGTATTCTAATAGAAAATTATGCTGGGGACTTCCCATTATGGCTTTCTCCAATCCAAGTTCGAGTTTTGCCCGTAACGGATGCCCAGCTCTCATACTGCAAAGAGGTGAGCGAAAAACTCAGAGCCAGTGGAGTTCGAGCAGAAGTTTGTCACGGTGAGCGGTTGCCTAAGCTTATTAGAAATGCAGAGACCCAAAAAATACCATTGATGGCAGTTGTTGGTGCCAAGGAAGTCGAGATGCAAACTGTGACAGTGAGATCAAGGTTTGGTGGGGAGATAGGGACCATAGCAGTTGATGACTTTGTTGGAAGAATCAAATTCGCCTCAGAGAATAGAACTCCATTTTGA

Protein sequence

MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTSISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVGRIKFASENRTPF*
BLAST of Cucsa.100510 vs. Swiss-Prot
Match: SYTM2_ARATH (Threonine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana GN=EMB2761 PE=2 SV=1)

HSP 1 Score: 1057.7 bits (2734), Expect = 5.1e-308
Identity = 513/664 (77.26%), Postives = 585/664 (88.10%), Query Frame = 1

Query: 7   MATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTSISVATD 66
           MA++  LL  S  ++  S   S+ R F+ + T       R F PR + +GFST  +VAT+
Sbjct: 1   MASSHSLLFSSSFLSKPSSFTSSLRRFVYLPT-------RQFWPRQR-HGFSTVFAVATE 60

Query: 67  SSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVT 126
              P+IS+         K +   +VLP+NESS+KLL+IRHTCAHVMAMAVQKLFPDAKVT
Sbjct: 61  ---PAISSSGPK-----KAEPSTVVLPSNESSDKLLKIRHTCAHVMAMAVQKLFPDAKVT 120

Query: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 186
           IGPWI+NGFYYDFDMEPL DKDLK+IKKEM+RIISRNLPL+REEVSR+EA++RI AINEP
Sbjct: 121 IGPWIDNGFYYDFDMEPLTDKDLKRIKKEMDRIISRNLPLLREEVSREEAKKRIMAINEP 180

Query: 187 YKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246
           YKME+L+ IKE+PIT+YHIGNEWWDLCAGPHVE+TGKINK+AVELE+VAGAYWRGDEK  
Sbjct: 181 YKMEILDGIKEEPITVYHIGNEWWDLCAGPHVETTGKINKKAVELESVAGAYWRGDEKRQ 240

Query: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 306
           MLQRIYGTAWE+EEQLKAYLHFKEEAKRRDHRR+G+DLDLFSIQD+AGGGLVFWHPKGA+
Sbjct: 241 MLQRIYGTAWESEEQLKAYLHFKEEAKRRDHRRIGQDLDLFSIQDEAGGGLVFWHPKGAI 300

Query: 307 VRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRP 366
           VR+IIE+ WKK+H E GYD++YTPHVAKA+LWK SGH DFY ENMY+QM++E+ELYQLRP
Sbjct: 301 VRNIIEESWKKMHVEHGYDLIYTPHVAKADLWKISGHLDFYRENMYDQMEIEDELYQLRP 360

Query: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDD 426
           MNCPYHIL Y+R+  SY+D PIRVAELGTVYRYELSGSL GLFRVRGFTQDDAHIFCL+D
Sbjct: 361 MNCPYHILLYQRKRQSYRDLPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLED 420

Query: 427 QIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDALGDKGWG 486
           QIKDEIRGVLDLTE++L +FGFN++EVNLSTRP KSVG D+IWEKAT AL+DAL DKGW 
Sbjct: 421 QIKDEIRGVLDLTEEILSRFGFNKYEVNLSTRPEKSVGGDDIWEKATCALRDALDDKGWS 480

Query: 487 YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIH 546
           Y++DEGGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQRFDITYVD+NS+KKRPIMIH
Sbjct: 481 YEVDEGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQRFDITYVDTNSDKKRPIMIH 540

Query: 547 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRASGVRAEV 606
           RAVLGSLERFFG+LIE+YAGDFPLWLSP+QVRVLPVTD QL +CKEVS+KLRA GVRAE+
Sbjct: 541 RAVLGSLERFFGVLIEHYAGDFPLWLSPVQVRVLPVTDNQLEFCKEVSKKLRACGVRAEL 600

Query: 607 CHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVGRIKFAS 666
           CHGERLPKLIRNAETQKIPLMAVVG KEVE  TVTVRSRFGGE+GTI VDD + +I  A 
Sbjct: 601 CHGERLPKLIRNAETQKIPLMAVVGPKEVETGTVTVRSRFGGELGTIPVDDLINKINIAV 648

Query: 667 ENRT 671
           E RT
Sbjct: 661 ETRT 648

BLAST of Cucsa.100510 vs. Swiss-Prot
Match: SYT_SYNPW (Threonine--tRNA ligase OS=Synechococcus sp. (strain WH7803) GN=thrS PE=3 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 1.8e-220
Identity = 362/582 (62.20%), Postives = 457/582 (78.52%), Query Frame = 1

Query: 90  IVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDM-EPLGDKD 149
           +VLP    +E+LLRIRH+ +HVMAMAVQ+LFP+A+VTIGPW E+GFYYDFD  EP  + D
Sbjct: 23  VVLPKTSENEQLLRIRHSMSHVMAMAVQQLFPNARVTIGPWTESGFYYDFDNPEPFTEAD 82

Query: 150 LKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMELLESIKEDPITIYHIGNE 209
           LK IKK M +II++ LPL R EVSR EA+ +I+A NEPYK+E+LE ++E PIT+Y +G +
Sbjct: 83  LKAIKKGMIKIINKKLPLERVEVSRSEAEEKIKAQNEPYKLEILEGLQE-PITLYTLGED 142

Query: 210 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 269
           WWDLCAGPHVE+TG++N +A ELE+VAGAYWRGDE    LQRIYGTAWET EQL  +   
Sbjct: 143 WWDLCAGPHVENTGQLNAKAFELESVAGAYWRGDETKAQLQRIYGTAWETAEQLAEHKRR 202

Query: 270 KEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLY 329
           KEEA RRDHRR+GKDLDLFSI+D+AG GLVFWHP+GA +R +IE+ W++ H E GY++LY
Sbjct: 203 KEEALRRDHRRIGKDLDLFSIEDEAGAGLVFWHPRGARMRLLIEEFWRQAHFEGGYELLY 262

Query: 330 TPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPI 389
           TPHVA   LWKTSGH DFY E+M+  M+V+   YQL+PMNCP+H+L Y  +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMEVDEREYQLKPMNCPFHVLTYASKLRSYRELPI 322

Query: 390 RVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGF 449
           R AELGTVYRYE  G + GL RVRGFTQDDAH+FCL +QI DEI  +LDLTE +L  F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPEQISDEILKILDLTERILSTFDF 382

Query: 450 NEFEVNLSTRPPKSVGNDEIWEKATSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDA 509
           N +E+NLSTRP KS+G+D +W+ AT  L +AL  KGW Y+IDEGGGAFYGPKID+KIEDA
Sbjct: 383 NTYEINLSTRPEKSIGDDAVWDLATKGLIEALERKGWAYKIDEGGGAFYGPKIDLKIEDA 442

Query: 510 LGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDF 569
           +GR WQCSTIQ+DFNLP+RF + Y+ ++  K+RPIMIHRA+ GSLERFFGI+ ENYAGD+
Sbjct: 443 IGRMWQCSTIQLDFNLPERFKLDYIAADGSKQRPIMIHRAIFGSLERFFGIMTENYAGDY 502

Query: 570 PLWLSPIQVRVLPVTDAQLSYCKEVSEKLRASGVRAEVCH-GERLPKLIRNAETQKIPLM 629
           PLWL+P QVR+LPVTD    Y + + ++L  +GVRA +   G+RL KLIR  E  KIP++
Sbjct: 503 PLWLAPEQVRLLPVTDEVQPYAESLLDQLTQAGVRATIDRSGDRLGKLIRTGEQMKIPVL 562

Query: 630 AVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVGRIKFASENR 670
           AV+GAKE E   V++RSR  G++G +AV D +   + A+  R
Sbjct: 563 AVIGAKEAEQNAVSLRSRRDGDLGVVAVADLLRAAQNANSQR 603

BLAST of Cucsa.100510 vs. Swiss-Prot
Match: SYT_SYNS3 (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9311) GN=thrS PE=3 SV=1)

HSP 1 Score: 766.1 bits (1977), Expect = 3.1e-220
Identity = 361/582 (62.03%), Postives = 454/582 (78.01%), Query Frame = 1

Query: 90  IVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDM-EPLGDKD 149
           +VLP    SE LL+IRH+ +HVMAMAVQKLFP A+VTIGPW E GFYYDFD  EP  + D
Sbjct: 23  VVLPKTSESENLLKIRHSMSHVMAMAVQKLFPKAQVTIGPWTEAGFYYDFDNPEPFTEAD 82

Query: 150 LKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMELLESIKEDPITIYHIGNE 209
           LK IKKEM +II R LPL R EVSR+EA+RRI+A NEPYK+E+LE + E PIT+Y +G +
Sbjct: 83  LKAIKKEMGKIIGRKLPLERIEVSREEAERRIKAQNEPYKLEILERLVE-PITLYTLGEQ 142

Query: 210 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 269
           WWDLCAGPHV +T ++N +A ELE+VAGAYWRGDE    LQRIYGTAWET +QL  +   
Sbjct: 143 WWDLCAGPHVANTSELNPKAFELESVAGAYWRGDETKAQLQRIYGTAWETADQLSEHKRR 202

Query: 270 KEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLY 329
           KEEA RRDHRRLGKDLDLFSI+D+AG GLVFWHP+GA +R +IED W++ H E GY++LY
Sbjct: 203 KEEALRRDHRRLGKDLDLFSIEDEAGAGLVFWHPRGARMRLLIEDFWRQAHFEGGYELLY 262

Query: 330 TPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPI 389
           TPHVA   LWKTSGH DFY E+M+  M+V+   YQL+PMNCP+H+L Y  +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMEVDERQYQLKPMNCPFHVLTYASKLRSYRELPI 322

Query: 390 RVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGF 449
           R AELGTVYRYE  G + GL RVRGFTQDDAH+FCL +QI DEI  +L+LTE +L  F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPEQISDEILRILNLTERILSTFDF 382

Query: 450 NEFEVNLSTRPPKSVGNDEIWEKATSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDA 509
           + +E+NLST+P K++G+D +WE AT  L +AL  KGW Y+IDEGGGAFYGPKID+KIEDA
Sbjct: 383 SNYEINLSTKPDKAIGDDAVWELATKGLIEALKRKGWAYKIDEGGGAFYGPKIDLKIEDA 442

Query: 510 LGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDF 569
           +GR WQCSTIQ+DFNLP+RF++ Y+ ++  K+RPIMIHRA+ GSLERFFGI+ ENYAGDF
Sbjct: 443 IGRMWQCSTIQLDFNLPERFELDYIAADGSKQRPIMIHRAIFGSLERFFGIMTENYAGDF 502

Query: 570 PLWLSPIQVRVLPVTDAQLSYCKEVSEKLRASGVRAEVCH-GERLPKLIRNAETQKIPLM 629
           P WL+P Q+R+LPVTD  L Y +E   +L+A+G+RA +   G+RL KLIR  E  KIP++
Sbjct: 503 PFWLAPEQIRLLPVTDEVLGYAEEFQNQLKAAGIRASIDRSGDRLGKLIRIGEKMKIPVL 562

Query: 630 AVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVGRIKFASENR 670
           AV+GAKE E    ++RSR  G++G I  +  +   + A+++R
Sbjct: 563 AVIGAKEAEQGAASLRSRRDGDLGVITKERLIATAQSANQDR 603

BLAST of Cucsa.100510 vs. Swiss-Prot
Match: SYT_SYNS9 (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9902) GN=thrS PE=3 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 5.3e-220
Identity = 362/584 (61.99%), Postives = 455/584 (77.91%), Query Frame = 1

Query: 90  IVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDM-EPLGDKD 149
           +VLP    SE+LL+IRH+ +HVMAMAVQKLFP+A+VTIGPW E GFYYDFD  EP  + D
Sbjct: 23  VVLPKTSESEQLLKIRHSMSHVMAMAVQKLFPNAQVTIGPWTETGFYYDFDNPEPFTEDD 82

Query: 150 LKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMELLESIKEDPITIYHIGNE 209
           LK IKKEM +II R LPL R EVSRDEA+ RI+A NEPYK+E+LE ++E PIT+Y +G++
Sbjct: 83  LKAIKKEMGKIIGRKLPLERIEVSRDEAETRIKAQNEPYKLEILERLQE-PITLYTLGDQ 142

Query: 210 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 269
           WWDLCAGPHVE+T +++ +A ELE+VAGAYWRGDE    LQRIYGTAWET EQL  +   
Sbjct: 143 WWDLCAGPHVENTKELHPKAFELESVAGAYWRGDETKAQLQRIYGTAWETPEQLAEHKRR 202

Query: 270 KEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLY 329
           K EA RRDHRRLGKDL+LFSI+D+AG GLVFWHP+GA +R +IED W++ H E GY++LY
Sbjct: 203 KVEALRRDHRRLGKDLELFSIEDEAGAGLVFWHPRGARMRLLIEDFWRQAHFEGGYELLY 262

Query: 330 TPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPI 389
           TPHVA   LWKTSGH DFY E+M+  M V+   YQL+PMNCP+H+L Y  +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMQVDEREYQLKPMNCPFHVLTYASKLRSYRELPI 322

Query: 390 RVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGF 449
           R AELGTVYRYE  G + GL RVRGFTQDDAH+FCL DQI DEI  +LDLTE +L  F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPDQISDEILRILDLTERILSTFDF 382

Query: 450 NEFEVNLSTRPPKSVGNDEIWEKATSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDA 509
           N +E+NLSTRP KS+G++ +WE AT  L +AL  KGW ++IDEGGGAFYGPKID+KIEDA
Sbjct: 383 NHYEINLSTRPEKSIGSEAVWELATKGLTEALERKGWNFKIDEGGGAFYGPKIDLKIEDA 442

Query: 510 LGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDF 569
           +GR WQCSTIQ+DFNLP+RF + YV ++  K++PIMIHRA+ GSLERFFGI+ ENYAGDF
Sbjct: 443 IGRMWQCSTIQLDFNLPERFGLDYVAADGSKQQPIMIHRAIFGSLERFFGIMTENYAGDF 502

Query: 570 PLWLSPIQVRVLPVTDAQLSYCKEVSEKLRASGVRAEVCH-GERLPKLIRNAETQKIPLM 629
           P WL+P Q+R+LPVTD    Y ++V  +L+ +G+RA +   G+RL K+IR  E  KIP++
Sbjct: 503 PFWLAPEQIRLLPVTDDVQPYAEQVLNQLKTAGIRATIDQSGDRLGKIIRTGEQMKIPVL 562

Query: 630 AVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVGRIKFASENRTP 672
           AV+GAKE E   +++RSR  G++G  +VD  +   + A+  R P
Sbjct: 563 AVIGAKEAEQNAISLRSRRDGDLGVTSVDALLKAAQRANAERQP 605

BLAST of Cucsa.100510 vs. Swiss-Prot
Match: SYT_SYNSC (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9605) GN=thrS PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 8.4e-218
Identity = 355/582 (61.00%), Postives = 453/582 (77.84%), Query Frame = 1

Query: 90  IVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDM-EPLGDKD 149
           +VLP    S++LL+IRH+ +HVMAMAVQ+LFP A+VTIGPW E GFYYDFD  +P  + D
Sbjct: 23  VVLPKTSESDQLLKIRHSMSHVMAMAVQQLFPKARVTIGPWTETGFYYDFDNPDPFTEAD 82

Query: 150 LKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMELLESIKEDPITIYHIGNE 209
           LK IKK M +II++ LPL R EVSR+EA+ +I+A NEPYK+E+L+ + E PIT+Y +G +
Sbjct: 83  LKAIKKGMIKIINKKLPLQRVEVSRNEAEEKIKAQNEPYKLEILQGLHE-PITLYTLGED 142

Query: 210 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 269
           WWDLCAGPHV+ TG++N +A ELE++AGAYWRGDE    LQRIYGTAWE+ EQL  +   
Sbjct: 143 WWDLCAGPHVDHTGQLNAKAFELESLAGAYWRGDETKAQLQRIYGTAWESPEQLAEHKRR 202

Query: 270 KEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLY 329
           KEEA RRDHRR+GKDLDLFSI+D+AG GLVFWHP+GA +R +IE+ W++ H E GY++LY
Sbjct: 203 KEEALRRDHRRIGKDLDLFSIEDEAGAGLVFWHPRGARIRLLIEEFWRQAHFEGGYELLY 262

Query: 330 TPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPI 389
           TPHVA   LWKTSGH DFY E+M+  M+V+   YQL+PMNCP+H+L Y  +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMEVDEREYQLKPMNCPFHVLTYASKLRSYRELPI 322

Query: 390 RVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGF 449
           R AELGTVYRYE  G + GL RVRGFTQDDAH+FCL +QI DEI  +LDLTE +L  F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPEQISDEILKILDLTERILSAFDF 382

Query: 450 NEFEVNLSTRPPKSVGNDEIWEKATSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDA 509
           + +E+NLSTRP KS+G D +W+ AT  L +AL  KGW Y+IDEGGGAFYGPKID+KIEDA
Sbjct: 383 SNYEINLSTRPEKSIGEDAVWDLATKGLIEALERKGWAYKIDEGGGAFYGPKIDLKIEDA 442

Query: 510 LGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDF 569
           +GR WQCSTIQ+DFNLP+RF++ Y+ ++  K+RPIMIHRA+ GSLERFFGI+ ENYAGD+
Sbjct: 443 IGRMWQCSTIQLDFNLPERFELDYIAADGSKQRPIMIHRAIFGSLERFFGIMTENYAGDY 502

Query: 570 PLWLSPIQVRVLPVTDAQLSYCKEVSEKLRASGVRAEVCH-GERLPKLIRNAETQKIPLM 629
           P WL+P QVR+LPVTD    Y +++ ++L  +GVRA V   G+RL KLIR  E  KIP++
Sbjct: 503 PFWLAPEQVRLLPVTDEVQPYAEQLLDQLTKAGVRATVDRSGDRLGKLIRTGEQMKIPVL 562

Query: 630 AVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVGRIKFASENR 670
           AV+GAKE E   V++RSR  G++G  AV D +   + A+  R
Sbjct: 563 AVIGAKEAEQNAVSLRSRRDGDLGVTAVSDLLSAAQMANSER 603

BLAST of Cucsa.100510 vs. TrEMBL
Match: A0A0A0K179_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G033430 PE=3 SV=1)

HSP 1 Score: 1365.9 bits (3534), Expect = 0.0e+00
Identity = 672/672 (100.00%), Postives = 672/672 (100.00%), Query Frame = 1

Query: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS 60
           MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS
Sbjct: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS 60

Query: 61  ISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           ISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61  ISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 EAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
           EAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
           HPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480
           IFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480

Query: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
           GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK
Sbjct: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540

Query: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS 600
           RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS
Sbjct: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS 600

Query: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVG 660
           GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVG
Sbjct: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVG 660

Query: 661 RIKFASENRTPF 673
           RIKFASENRTPF
Sbjct: 661 RIKFASENRTPF 672

BLAST of Cucsa.100510 vs. TrEMBL
Match: W9SJL8_9ROSA (Threonine--tRNA ligase OS=Morus notabilis GN=L484_020038 PE=3 SV=1)

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 542/691 (78.44%), Postives = 605/691 (87.55%), Query Frame = 1

Query: 1   MFVLQRMATTTGLLL-HSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFST 60
           MF+L RMAT+  L    S  + P SP+ S  R  L ++ +F     R F      NGFST
Sbjct: 1   MFILHRMATSQSLFAPSSSFLKPSSPS-SFNRLGLPLTRQF-----RAFALHGHRNGFST 60

Query: 61  SIS-VATDSSAPSISTQNEDLV---DAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMA 120
           + S VAT+SSA + S +N +L    D +  + E++VLPTN+SSEKLLRIRHTCAHVMAMA
Sbjct: 61  AYSAVATESSAET-SVENLELETNGDENSQKEERVVLPTNDSSEKLLRIRHTCAHVMAMA 120

Query: 121 VQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDE 180
           VQKLFPDAKVTIGPWIENGFYYDFDMEPL D+DLKKIKKEM+RI+ +NLPL+REEVSRDE
Sbjct: 121 VQKLFPDAKVTIGPWIENGFYYDFDMEPLTDRDLKKIKKEMDRIVGKNLPLIREEVSRDE 180

Query: 181 AQRRIEAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVA 240
           AQRRI ++NEPYK+E+LESIKE+PITIYHIGNEWWDLCAGPHVESTGKIN+RAVELE+VA
Sbjct: 181 AQRRIMSLNEPYKLEILESIKEEPITIYHIGNEWWDLCAGPHVESTGKINRRAVELESVA 240

Query: 241 GAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGG 300
           GAYWRGDEK PMLQRIYGTAWE E+QLKAYLHFKEEAKRRDHRRLG+DLDLFSIQ+DAGG
Sbjct: 241 GAYWRGDEKKPMLQRIYGTAWENEQQLKAYLHFKEEAKRRDHRRLGQDLDLFSIQNDAGG 300

Query: 301 GLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQM 360
           GLVFWHPKGAVVR++IEDLW+K+H ERGYD+LYTPHVAKA+LW+ SGH DFY ENMY+QM
Sbjct: 301 GLVFWHPKGAVVRNVIEDLWRKIHIERGYDLLYTPHVAKADLWQISGHLDFYRENMYDQM 360

Query: 361 DVENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFT 420
           ++E+ELYQLRPMNCPYHIL YKR+L SYQDFPIRVAELGTVYRYELSGSL GLFRVRGFT
Sbjct: 361 NIEDELYQLRPMNCPYHILIYKRKLHSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFT 420

Query: 421 QDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSA 480
           QDDAHIFCL DQIKDEIRGVLDLTE++LLQFGF+++EVNLSTRP KSVG+D+IWEKATSA
Sbjct: 421 QDDAHIFCLSDQIKDEIRGVLDLTEEMLLQFGFSKYEVNLSTRPEKSVGDDDIWEKATSA 480

Query: 481 LKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ--------------VD 540
           LKDAL DKGW YQIDEGGGAFYGPKID+KIEDALGRKWQCSTIQ              VD
Sbjct: 481 LKDALDDKGWSYQIDEGGGAFYGPKIDLKIEDALGRKWQCSTIQAIPRLIFSRFYGKRVD 540

Query: 541 FNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLP 600
           FNLPQRFDITYVDS SEKKRPIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSPIQ RVLP
Sbjct: 541 FNLPQRFDITYVDSTSEKKRPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPIQARVLP 600

Query: 601 VTDAQLSYCKEVSEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVT 660
           VTD QL YC EV++KL+A+G+RAEVCHGERLPKLIRNAETQKIPLMAVVGAKE+E  TVT
Sbjct: 601 VTDTQLEYCNEVAKKLKANGIRAEVCHGERLPKLIRNAETQKIPLMAVVGAKELETGTVT 660

Query: 661 VRSRFGGEIGTIAVDDFVGRIKFASENRTPF 673
           VRSRFGG++GT+ VDDFVGR K A ENRT F
Sbjct: 661 VRSRFGGDVGTMTVDDFVGRTKSAIENRTSF 684

BLAST of Cucsa.100510 vs. TrEMBL
Match: A0A067K3R0_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_13804 PE=3 SV=1)

HSP 1 Score: 1071.6 bits (2770), Expect = 3.6e-310
Identity = 511/618 (82.69%), Postives = 568/618 (91.91%), Query Frame = 1

Query: 53  KSNGFSTSISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVM 112
           K NG S S +VAT+S+    + +N+ + +  +++TEKIVLPTNESSEKLLRIRHTCAHVM
Sbjct: 56  KRNGLSMSTAVATESAT---AARNDVVEETQREETEKIVLPTNESSEKLLRIRHTCAHVM 115

Query: 113 AMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVS 172
           AMAVQKL+P+AKVTIGPWI+NGFYYDFDMEPL DKDLKKIKKEM+RII RNLPLVREEVS
Sbjct: 116 AMAVQKLYPEAKVTIGPWIDNGFYYDFDMEPLTDKDLKKIKKEMDRIIGRNLPLVREEVS 175

Query: 173 RDEAQRRIEAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELE 232
           RDEA+RRI AINEPYKME+LESIKEDPITIYHIG EWWDLCAGPHVE+TG INK++VELE
Sbjct: 176 RDEAKRRIMAINEPYKMEILESIKEDPITIYHIGAEWWDLCAGPHVETTGLINKKSVELE 235

Query: 233 TVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDD 292
           +VAGAYWRGDEKNPMLQRIYGTAWE E+QLKAYLHFKEEAKRRDHRRLG+DLDLFSIQD+
Sbjct: 236 SVAGAYWRGDEKNPMLQRIYGTAWENEQQLKAYLHFKEEAKRRDHRRLGQDLDLFSIQDE 295

Query: 293 AGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMY 352
           AGGGLVFWHPKGA+VR IIED WKK H + GYD+LYTPHVAKAELWK SGH DFY ENMY
Sbjct: 296 AGGGLVFWHPKGAIVRHIIEDSWKKTHIKHGYDLLYTPHVAKAELWKISGHLDFYRENMY 355

Query: 353 NQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVR 412
           +QM +E+E YQLRPMNCPYHIL +KR+L SY+DFPIRVAELGTVYRYELSGSL GLFRVR
Sbjct: 356 DQMQIEDENYQLRPMNCPYHILVFKRKLNSYRDFPIRVAELGTVYRYELSGSLHGLFRVR 415

Query: 413 GFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKA 472
           GFTQDDAHIFCL+DQIKDEIRGVLDLTE++LLQFGF+++EVNLSTRP K VG D+IWEKA
Sbjct: 416 GFTQDDAHIFCLEDQIKDEIRGVLDLTEEILLQFGFSKYEVNLSTRPEKFVGGDDIWEKA 475

Query: 473 TSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITY 532
           TSAL+DAL DKGW YQIDEGGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQRFDITY
Sbjct: 476 TSALRDALEDKGWNYQIDEGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQRFDITY 535

Query: 533 VDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKE 592
           VDSN+EKK+PIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSPIQVRVLPVTD QL YCKE
Sbjct: 536 VDSNTEKKQPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPIQVRVLPVTDTQLEYCKE 595

Query: 593 VSEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGT 652
           V+ KL+ + +R EVCHGERLPKLIRNAE QKIPLMAVVGAKEVE Q+VT+RSRF GE+GT
Sbjct: 596 VTNKLKGNDIRTEVCHGERLPKLIRNAEKQKIPLMAVVGAKEVETQSVTIRSRFSGELGT 655

Query: 653 IAVDDFVGRIKFASENRT 671
           + +DDF+ RIKFA + RT
Sbjct: 656 MKIDDFISRIKFAVDKRT 670

BLAST of Cucsa.100510 vs. TrEMBL
Match: M5WYT6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002440mg PE=3 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 1.1e-309
Identity = 524/680 (77.06%), Postives = 597/680 (87.79%), Query Frame = 1

Query: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFL--------AVSTKFARNRDRPFLPRN 60
           M +LQRMAT++  +  +PL+   S   S P  FL        A ST+F        L RN
Sbjct: 1   MLILQRMATSSSFI-STPLLKASS---SFPTHFLSPLKHCVSAPSTEFRALA----LYRN 60

Query: 61  KSNGFSTSISVATDSSAPSISTQNEDLVDAHK---DQTEKIVLPTNESSEKLLRIRHTCA 120
           ++   ++S +VAT++    +STQ++ L +  K   D+ EK+VLPTNESSE LLRIRHTCA
Sbjct: 61  RNGVSTSSAAVATETQ---VSTQDDKLKETQKEKSDKLEKVVLPTNESSESLLRIRHTCA 120

Query: 121 HVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVRE 180
           HVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPL DK+LK+IKKEM+RII +NLPL+RE
Sbjct: 121 HVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLTDKELKRIKKEMDRIIGKNLPLIRE 180

Query: 181 EVSRDEAQRRIEAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAV 240
           E+SRDEAQRRI A+NEPYK+E+L+SIKEDPITIYHIGNEWWDLCAGPHVE TGKIN++AV
Sbjct: 181 EISRDEAQRRITALNEPYKLEILDSIKEDPITIYHIGNEWWDLCAGPHVEYTGKINRKAV 240

Query: 241 ELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSI 300
           ELE++AGAYWRGDEK PMLQRIYGTAWE+E+QLKAYLHFKEEAKRRDHRRLG+ LDLFSI
Sbjct: 241 ELESIAGAYWRGDEKKPMLQRIYGTAWESEDQLKAYLHFKEEAKRRDHRRLGQVLDLFSI 300

Query: 301 QDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGE 360
           Q+DAGGGLVFWHPKGA+VR +IEDLWKK+H ERGYD+LYTPHVAKA+LW+ SGH DFY E
Sbjct: 301 QNDAGGGLVFWHPKGAIVRHVIEDLWKKIHIERGYDLLYTPHVAKADLWRISGHLDFYKE 360

Query: 361 NMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLF 420
           NMY+QM++E+ELYQLRPMNCPYHIL YKRR  SY DFPIRVAELGTVYRYELSGSL GLF
Sbjct: 361 NMYDQMNIEDELYQLRPMNCPYHILIYKRRPHSYHDFPIRVAELGTVYRYELSGSLHGLF 420

Query: 421 RVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIW 480
           RVRGFTQDDAHIFCL+DQIKDEIRGVLDLTE+LLLQFGF+++EVNLSTRP KSVG+D+IW
Sbjct: 421 RVRGFTQDDAHIFCLEDQIKDEIRGVLDLTEELLLQFGFSKYEVNLSTRPEKSVGDDDIW 480

Query: 481 EKATSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFD 540
            KATSAL+DAL DKGW YQID+GGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQRFD
Sbjct: 481 VKATSALRDALDDKGWSYQIDDGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQRFD 540

Query: 541 ITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSY 600
           ITYVDSNSEKKRPIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSP+Q  VLPVTD QL Y
Sbjct: 541 ITYVDSNSEKKRPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPVQAHVLPVTDTQLDY 600

Query: 601 CKEVSEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGE 660
           CKEV+ KL+A+G+R E+CHGERLPKLIRN+E  KIPLMAVVGAKEVE  +VTVRSRFGG+
Sbjct: 601 CKEVTNKLKANGIRGELCHGERLPKLIRNSEMLKIPLMAVVGAKEVETGSVTVRSRFGGD 660

Query: 661 IGTIAVDDFVGRIKFASENR 670
           +GT+A+DDFV  IK A E+R
Sbjct: 661 LGTMAIDDFVSTIKSAIESR 669

BLAST of Cucsa.100510 vs. TrEMBL
Match: B9IAN8_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s16030g PE=3 SV=2)

HSP 1 Score: 1063.1 bits (2748), Expect = 1.4e-307
Identity = 518/684 (75.73%), Postives = 580/684 (84.80%), Query Frame = 1

Query: 1   MFVLQRMATTTGLLLHS-----------PLVTPISPNVSAPRFFLAVSTKFARNRDRPFL 60
           MFVL +MAT+    L S           PL+ P   ++ +P     +ST      D    
Sbjct: 1   MFVLHKMATSPSSSLCSFSLFLSKPSSLPLLKPPLLSLHSPPLKRFISTASELQHDTQAR 60

Query: 61  PRNKSNGFSTSISVATDSSAPSISTQNEDLVD---AHKDQTEKIVLPTNESSEKLLRIRH 120
            R       T  S     S  +I+T++  +     A  ++TEK+VLPTN+SSEKLLRIRH
Sbjct: 61  RRTLLGSPYTKTSPLLSLSTAAIATESATVSHNDTAENEETEKLVLPTNDSSEKLLRIRH 120

Query: 121 TCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL 180
           TCAHVMAMAVQKL+P+AKVTIGPWIENGFYYDFDM+PL DKDLK+IKKEM+RII RNLPL
Sbjct: 121 TCAHVMAMAVQKLYPEAKVTIGPWIENGFYYDFDMDPLTDKDLKRIKKEMDRIIGRNLPL 180

Query: 181 VREEVSRDEAQRRIEAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINK 240
            RE+VSRDEAQRRI A+NEPYKME+LESIKEDPITIYHIG+EWWDLCAGPHVESTG IN+
Sbjct: 181 AREDVSRDEAQRRIMAVNEPYKMEILESIKEDPITIYHIGDEWWDLCAGPHVESTGNINR 240

Query: 241 RAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDL 300
           +AVELE+VAGAYWRGDEK PMLQRIYGTAWE E+QLKAYLHFKEEAKRRDHRRLG+DLDL
Sbjct: 241 KAVELESVAGAYWRGDEKKPMLQRIYGTAWENEDQLKAYLHFKEEAKRRDHRRLGQDLDL 300

Query: 301 FSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDF 360
           FSIQD+AGGGLVFWHPKGAV+R IIED WKK H E GYD+LYTPHVAKA+LWK SGH DF
Sbjct: 301 FSIQDEAGGGLVFWHPKGAVIRHIIEDSWKKTHIEHGYDLLYTPHVAKADLWKISGHLDF 360

Query: 361 YGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLR 420
           Y ENM++QM +E+E YQLRPMNCPYHIL YKR+L SY+DFPIRVAELGTVYRYELSGSL 
Sbjct: 361 YRENMFDQMKIEDEQYQLRPMNCPYHILLYKRKLNSYRDFPIRVAELGTVYRYELSGSLH 420

Query: 421 GLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGND 480
           GLFRVRGFTQDDAHIFCL+DQIKDEIRGVLDLTE++LLQFGF  +EVNLSTRP KSVG+D
Sbjct: 421 GLFRVRGFTQDDAHIFCLEDQIKDEIRGVLDLTEEILLQFGFRRYEVNLSTRPEKSVGDD 480

Query: 481 EIWEKATSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQ 540
           +IWEKATSAL+DAL DKGW +QIDEGGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQ
Sbjct: 481 DIWEKATSALRDALDDKGWSFQIDEGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQ 540

Query: 541 RFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQ 600
           RFD+TYVDSN+EKKRPIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSP+QVR+LPVTD Q
Sbjct: 541 RFDVTYVDSNTEKKRPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPVQVRILPVTDTQ 600

Query: 601 LSYCKEVSEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRF 660
           L YCKEV  KL+ +G+RAEVC GERLPKLIRNAE QKIPLMAVVG KE E QTVT+RSRF
Sbjct: 601 LEYCKEVISKLKGNGIRAEVCQGERLPKLIRNAEKQKIPLMAVVGPKEAETQTVTIRSRF 660

Query: 661 GGEIGTIAVDDFVGRIKFASENRT 671
            GE+GTI +DDF+ RIK A E RT
Sbjct: 661 SGEVGTIEIDDFISRIKSAIERRT 684

BLAST of Cucsa.100510 vs. TAIR10
Match: AT2G04842.1 (AT2G04842.1 threonyl-tRNA synthetase, putative / threonine--tRNA ligase, putative)

HSP 1 Score: 1057.7 bits (2734), Expect = 2.9e-309
Identity = 513/664 (77.26%), Postives = 585/664 (88.10%), Query Frame = 1

Query: 7   MATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTSISVATD 66
           MA++  LL  S  ++  S   S+ R F+ + T       R F PR + +GFST  +VAT+
Sbjct: 1   MASSHSLLFSSSFLSKPSSFTSSLRRFVYLPT-------RQFWPRQR-HGFSTVFAVATE 60

Query: 67  SSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVT 126
              P+IS+         K +   +VLP+NESS+KLL+IRHTCAHVMAMAVQKLFPDAKVT
Sbjct: 61  ---PAISSSGPK-----KAEPSTVVLPSNESSDKLLKIRHTCAHVMAMAVQKLFPDAKVT 120

Query: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 186
           IGPWI+NGFYYDFDMEPL DKDLK+IKKEM+RIISRNLPL+REEVSR+EA++RI AINEP
Sbjct: 121 IGPWIDNGFYYDFDMEPLTDKDLKRIKKEMDRIISRNLPLLREEVSREEAKKRIMAINEP 180

Query: 187 YKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246
           YKME+L+ IKE+PIT+YHIGNEWWDLCAGPHVE+TGKINK+AVELE+VAGAYWRGDEK  
Sbjct: 181 YKMEILDGIKEEPITVYHIGNEWWDLCAGPHVETTGKINKKAVELESVAGAYWRGDEKRQ 240

Query: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 306
           MLQRIYGTAWE+EEQLKAYLHFKEEAKRRDHRR+G+DLDLFSIQD+AGGGLVFWHPKGA+
Sbjct: 241 MLQRIYGTAWESEEQLKAYLHFKEEAKRRDHRRIGQDLDLFSIQDEAGGGLVFWHPKGAI 300

Query: 307 VRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRP 366
           VR+IIE+ WKK+H E GYD++YTPHVAKA+LWK SGH DFY ENMY+QM++E+ELYQLRP
Sbjct: 301 VRNIIEESWKKMHVEHGYDLIYTPHVAKADLWKISGHLDFYRENMYDQMEIEDELYQLRP 360

Query: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDD 426
           MNCPYHIL Y+R+  SY+D PIRVAELGTVYRYELSGSL GLFRVRGFTQDDAHIFCL+D
Sbjct: 361 MNCPYHILLYQRKRQSYRDLPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLED 420

Query: 427 QIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDALGDKGWG 486
           QIKDEIRGVLDLTE++L +FGFN++EVNLSTRP KSVG D+IWEKAT AL+DAL DKGW 
Sbjct: 421 QIKDEIRGVLDLTEEILSRFGFNKYEVNLSTRPEKSVGGDDIWEKATCALRDALDDKGWS 480

Query: 487 YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIH 546
           Y++DEGGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQRFDITYVD+NS+KKRPIMIH
Sbjct: 481 YEVDEGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQRFDITYVDTNSDKKRPIMIH 540

Query: 547 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRASGVRAEV 606
           RAVLGSLERFFG+LIE+YAGDFPLWLSP+QVRVLPVTD QL +CKEVS+KLRA GVRAE+
Sbjct: 541 RAVLGSLERFFGVLIEHYAGDFPLWLSPVQVRVLPVTDNQLEFCKEVSKKLRACGVRAEL 600

Query: 607 CHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVGRIKFAS 666
           CHGERLPKLIRNAETQKIPLMAVVG KEVE  TVTVRSRFGGE+GTI VDD + +I  A 
Sbjct: 601 CHGERLPKLIRNAETQKIPLMAVVGPKEVETGTVTVRSRFGGELGTIPVDDLINKINIAV 648

Query: 667 ENRT 671
           E RT
Sbjct: 661 ETRT 648

BLAST of Cucsa.100510 vs. TAIR10
Match: AT5G26830.1 (AT5G26830.1 Threonyl-tRNA synthetase)

HSP 1 Score: 399.4 bits (1025), Expect = 4.3e-111
Identity = 220/565 (38.94%), Postives = 338/565 (59.82%), Query Frame = 1

Query: 106 HTCAHVMAMAVQKLFPDAKVTIGPWIENG--FYYDFDMEPLG--DKDLKKIKKEMERIIS 165
           H+ AH++  A+++ +   ++ IGP    G  FYYD     LG  D     I+    +   
Sbjct: 147 HSSAHILGQALEQEY-GCQLCIGPCTTRGEGFYYDGFYGELGLSDNHFPSIEAGAAKAAK 206

Query: 166 RNLPLVREEVSRDEAQRRIEAINEPYKMELLESIKED-PITIYHIGNEWWDLCAGPHVES 225
              P  R EV++D+A       N  +K+EL+  +  D  IT+Y  G    DLC GPH+ +
Sbjct: 207 EAQPFERIEVTKDQALEMFSENN--FKVELINGLPADMTITVYRCG-PLVDLCRGPHIPN 266

Query: 226 TGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRL 285
           T  +  +A +    + AYW+GD+    LQR+YG ++  ++QLK YL F EEAK+ DHR L
Sbjct: 267 TSFV--KAFKCLRASSAYWKGDKDRESLQRVYGISYPDQKQLKKYLQFLEEAKKYDHRLL 326

Query: 286 GKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKT 345
           G+  +LF        G  F+ P G  V + + D  K  +  RGY  + TP++   ELW+T
Sbjct: 327 GQKQELF-FSHQLSPGSYFFLPLGTRVYNRLMDFIKNQYWHRGYTEVITPNMYNMELWQT 386

Query: 346 SGHSDFYGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYE 405
           SGH+D Y +NM+   ++E + + L+PMNCP H L ++ R+ SY++ P+R+A+ G ++R E
Sbjct: 387 SGHADNYKDNMFT-FNIEKQEFGLKPMNCPGHCLIFQHRVRSYRELPMRLADFGVLHRNE 446

Query: 406 LSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPP 465
            SG+L GL RVR F QDDAHIFC  +Q+K E++GVL+  + +   FGF  +E+ LSTRP 
Sbjct: 447 ASGALSGLTRVRRFQQDDAHIFCTTEQVKGEVQGVLEFIDYVYKVFGFT-YELKLSTRPE 506

Query: 466 KSVGNDEIWEKATSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQV 525
           K +G+ E W+KA + LK+A+   G    ++EG GAFYGPKIDI + DA+ RK+QC+T+Q+
Sbjct: 507 KYLGDLETWDKAEADLKEAIEAFGKPLVLNEGDGAFYGPKIDITVSDAMNRKFQCATLQL 566

Query: 526 DFNLPQRFDITY-VDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRV 585
           DF LP RF++ Y  +  ++K RP+MIHRAVLGS+ER F IL+E+Y G +P W+SP Q  V
Sbjct: 567 DFQLPIRFNLEYAAEDEAKKSRPVMIHRAVLGSVERMFAILLEHYKGKWPFWISPRQAIV 626

Query: 586 LPVTDAQLSYCKEVSEKLRASGVRAEVCHGER-LPKLIRNAETQKIPLMAVVGAKEVEMQ 645
            P+++    Y ++V ++++ +G   +    +R + K +R A+  +   + VVG  E    
Sbjct: 627 CPISEKSQQYAEKVQKQIKDAGFYVDADLTDRKIDKKVREAQLAQYNYILVVGETEAATG 686

Query: 646 TVTVRSRFGGEIGTIAVDDFVGRIK 664
            V+VR R        +++D +   K
Sbjct: 687 QVSVRVRDNAAHSVKSIEDLLEEFK 702

BLAST of Cucsa.100510 vs. TAIR10
Match: AT1G17960.1 (AT1G17960.1 Threonyl-tRNA synthetase)

HSP 1 Score: 233.4 bits (594), Expect = 4.1e-61
Identity = 127/287 (44.25%), Postives = 179/287 (62.37%), Query Frame = 1

Query: 387 PIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQF 446
           PIR+AE G ++R E SG+L G+ RVR F QDDAHIFC  DQ+++E++GVLD  + +   F
Sbjct: 171 PIRMAEFGVLHRNEDSGALGGMTRVRRFVQDDAHIFCRVDQVEEEVKGVLDFIDYVYRIF 230

Query: 447 GFNEFEVNLSTRPPKSVGNDEIWEKATSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIE 506
           GF  +E+ LSTRP   +G+ E W KA + L+ AL D G  + I EG GAFYGPKIDI + 
Sbjct: 231 GFT-YELTLSTRPKDHIGDLETWAKAENDLEKALDDFGKPWVIKEGDGAFYGPKIDITVS 290

Query: 507 DALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAG 566
           DA  RK+QC+TIQ+DF LP  F + Y+   +E + P+MIH AVLGS+ER F IL+E+Y G
Sbjct: 291 DARNRKFQCATIQLDFQLPACFKLKYLSEKNEMEAPVMIHSAVLGSIERMFAILLEHYKG 350

Query: 567 DFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRASGVRAEVCHGER-LPKLIRNAETQKIP 626
            +P WLSP Q  V  +++   SY K+V +++   G   ++   +R L K + +A      
Sbjct: 351 IWPFWLSPRQAIVCSLSEDCSSYAKQVQKQINEVGYYVDIDESDRSLRKKVADARDAPYN 410

Query: 627 LMAVVGAKEVEMQTVTVRSRFGGE----IGTIAVDDFVGRIKFASEN 669
            + VVG KEV    VTVR R   E    +  ++++  +   KF + N
Sbjct: 411 YILVVGQKEVATGQVTVRLREDPEGRKDLPEMSIESLLDEFKFKTVN 456

BLAST of Cucsa.100510 vs. TAIR10
Match: AT1G18130.1 (AT1G18130.1 Class II aaRS and biotin synthetases superfamily protein)

HSP 1 Score: 80.5 bits (197), Expect = 4.4e-15
Identity = 47/125 (37.60%), Postives = 69/125 (55.20%), Query Frame = 1

Query: 520 VDFNLPQRFDITYVDSNSEK-KRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVR 579
           VDF+L  RF + Y   N    +R ++IHRAVL S+ER F IL+E Y G FP WLSP Q  
Sbjct: 17  VDFHLRDRFKLEYSAENETMIERSVLIHRAVLSSVERLFAILLEQYKGKFPFWLSPRQAI 76

Query: 580 VLPVTDAQLSYCKEVSEKLRASGVRAEVCHGER-LPKLIRNAETQKIPLMAVVGAKEVEM 639
           V  V++   SY  +V +++  +G   +V   +R +   ++ A+      + VVG +E   
Sbjct: 77  VCSVSEDYRSYADKVRDQIHEAGYYVDVDTTDRNVSDKVQEAQVAHYNYILVVGDEEART 136

Query: 640 QTVTV 643
             VTV
Sbjct: 137 GQVTV 141

BLAST of Cucsa.100510 vs. NCBI nr
Match: gi|449453584|ref|XP_004144536.1| (PREDICTED: probable threonine--tRNA ligase, cytoplasmic [Cucumis sativus])

HSP 1 Score: 1365.9 bits (3534), Expect = 0.0e+00
Identity = 672/672 (100.00%), Postives = 672/672 (100.00%), Query Frame = 1

Query: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS 60
           MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS
Sbjct: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS 60

Query: 61  ISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           ISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61  ISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 EAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
           EAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
           HPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480
           IFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480

Query: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
           GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK
Sbjct: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540

Query: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS 600
           RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS
Sbjct: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS 600

Query: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVG 660
           GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVG
Sbjct: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVG 660

Query: 661 RIKFASENRTPF 673
           RIKFASENRTPF
Sbjct: 661 RIKFASENRTPF 672

BLAST of Cucsa.100510 vs. NCBI nr
Match: gi|659124339|ref|XP_008462105.1| (PREDICTED: probable threonine--tRNA ligase, cytoplasmic [Cucumis melo])

HSP 1 Score: 1337.0 bits (3459), Expect = 0.0e+00
Identity = 656/672 (97.62%), Postives = 663/672 (98.66%), Query Frame = 1

Query: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS 60
           MFVLQRMATTTGLLLHSPL+TPISPNVSAPRFFLAVSTKFA NRDR F PR KSN FSTS
Sbjct: 1   MFVLQRMATTTGLLLHSPLLTPISPNVSAPRFFLAVSTKFAGNRDRSFFPRKKSNVFSTS 60

Query: 61  ISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
           ISVATDSSAPS+STQNEDLVDAHKDQT KIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61  ISVATDSSAPSVSTQNEDLVDAHKDQTGKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120

Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
           PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180

Query: 181 EAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
            AINEPYKME+LESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRA+ELETVAGAYWR
Sbjct: 181 GAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAIELETVAGAYWR 240

Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
           GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEA+RRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFW 300

Query: 301 HPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
           HPKGAVVRSIIEDLWKKLHRERGYD+LYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360

Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
           LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420

Query: 421 IFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480
           IFCLDDQIKDEIRGVLDLTE+LLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480

Query: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
           GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK
Sbjct: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540

Query: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS 600
           RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS
Sbjct: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS 600

Query: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVG 660
           GVRAEVCH ERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTI VDDFVG
Sbjct: 601 GVRAEVCHSERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVG 660

Query: 661 RIKFASENRTPF 673
           RIKFASENRTPF
Sbjct: 661 RIKFASENRTPF 672

BLAST of Cucsa.100510 vs. NCBI nr
Match: gi|1009128110|ref|XP_015881052.1| (PREDICTED: threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Ziziphus jujuba])

HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 540/677 (79.76%), Postives = 601/677 (88.77%), Query Frame = 1

Query: 1   MFVLQRMATTTGLLLHSP-LVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFST 60
           MF+LQRMA +  L   S  L TP+    S P  F +  ++F        L  N  NGFST
Sbjct: 1   MFILQRMANSYSLFTSSAFLSTPLLKTSSLPSHFHSNLSRFVSTTPFNALFNNNRNGFST 60

Query: 61  SISVATDSSAP-SISTQNEDLVDAHKDQTE---KIVLPTNESSEKLLRIRHTCAHVMAMA 120
           S++VATDSS+  ++STQNE L     D+T+   K+VLPTN+SS+KLLRIRH+CAHVMAMA
Sbjct: 61  SLAVATDSSSQIAVSTQNEKLNQTQNDKTDDFRKVVLPTNDSSDKLLRIRHSCAHVMAMA 120

Query: 121 VQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDE 180
           VQK+FPDAKVTIGPWIENGFYYDFDM PL D+DLKKIKKEM+RII RNLPLVREEV+RDE
Sbjct: 121 VQKIFPDAKVTIGPWIENGFYYDFDMGPLTDRDLKKIKKEMDRIIGRNLPLVREEVTRDE 180

Query: 181 AQRRIEAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVA 240
           AQRRI A+NEPYK+E+L+SIKEDPITIYHIG+EWWDLCAGPHVESTGKIN+RAVELE+VA
Sbjct: 181 AQRRIMALNEPYKLEILDSIKEDPITIYHIGDEWWDLCAGPHVESTGKINRRAVELESVA 240

Query: 241 GAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGG 300
           GAYWRGDEKN MLQRIYGTAWE EEQLKAYLHFKEEAKRRDHRRLG+DLDLFSIQ++AGG
Sbjct: 241 GAYWRGDEKNQMLQRIYGTAWENEEQLKAYLHFKEEAKRRDHRRLGQDLDLFSIQNEAGG 300

Query: 301 GLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQM 360
           GLVFWHPKGA+VR +IEDLWKK+H ERGYDMLYTPHVAKA+LW+ SGH DFY ENMY+QM
Sbjct: 301 GLVFWHPKGAIVRHMIEDLWKKIHIERGYDMLYTPHVAKADLWQISGHLDFYKENMYDQM 360

Query: 361 DVENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFT 420
            +E+ELYQLRPMNCPYHIL YKR+L SY DFPIRVAELGTVYRYELSGSL GLFRVRGFT
Sbjct: 361 SIEDELYQLRPMNCPYHILIYKRKLHSYHDFPIRVAELGTVYRYELSGSLHGLFRVRGFT 420

Query: 421 QDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSA 480
           QDDAHIFCL DQIKDEIRGVLDLTE++LLQFGF+++EVNLSTRP K+VG D+IWEKATSA
Sbjct: 421 QDDAHIFCLSDQIKDEIRGVLDLTEEMLLQFGFSKYEVNLSTRPEKAVGGDDIWEKATSA 480

Query: 481 LKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDS 540
           LK+AL DKGW YQIDEGGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQRFDITYVDS
Sbjct: 481 LKEALDDKGWSYQIDEGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQRFDITYVDS 540

Query: 541 NSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSE 600
           NSEKKRPIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSPIQ R+LPVTD Q  YC EV++
Sbjct: 541 NSEKKRPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPIQARILPVTDTQREYCSEVAK 600

Query: 601 KLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAV 660
           KL+A+G+RAEVCHGERLPKLIRNAETQKIPLMAVVG KEVE  TVTVRSRF G+IGT+ V
Sbjct: 601 KLKANGIRAEVCHGERLPKLIRNAETQKIPLMAVVGPKEVETGTVTVRSRFAGDIGTLTV 660

Query: 661 DDFVGRIKFASENRTPF 673
           +DFVGR K A+ENRT F
Sbjct: 661 EDFVGRTKSATENRTSF 677

BLAST of Cucsa.100510 vs. NCBI nr
Match: gi|703161498|ref|XP_010112806.1| (Threonine--tRNA ligase [Morus notabilis])

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 542/691 (78.44%), Postives = 605/691 (87.55%), Query Frame = 1

Query: 1   MFVLQRMATTTGLLL-HSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFST 60
           MF+L RMAT+  L    S  + P SP+ S  R  L ++ +F     R F      NGFST
Sbjct: 1   MFILHRMATSQSLFAPSSSFLKPSSPS-SFNRLGLPLTRQF-----RAFALHGHRNGFST 60

Query: 61  SIS-VATDSSAPSISTQNEDLV---DAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMA 120
           + S VAT+SSA + S +N +L    D +  + E++VLPTN+SSEKLLRIRHTCAHVMAMA
Sbjct: 61  AYSAVATESSAET-SVENLELETNGDENSQKEERVVLPTNDSSEKLLRIRHTCAHVMAMA 120

Query: 121 VQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDE 180
           VQKLFPDAKVTIGPWIENGFYYDFDMEPL D+DLKKIKKEM+RI+ +NLPL+REEVSRDE
Sbjct: 121 VQKLFPDAKVTIGPWIENGFYYDFDMEPLTDRDLKKIKKEMDRIVGKNLPLIREEVSRDE 180

Query: 181 AQRRIEAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVA 240
           AQRRI ++NEPYK+E+LESIKE+PITIYHIGNEWWDLCAGPHVESTGKIN+RAVELE+VA
Sbjct: 181 AQRRIMSLNEPYKLEILESIKEEPITIYHIGNEWWDLCAGPHVESTGKINRRAVELESVA 240

Query: 241 GAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGG 300
           GAYWRGDEK PMLQRIYGTAWE E+QLKAYLHFKEEAKRRDHRRLG+DLDLFSIQ+DAGG
Sbjct: 241 GAYWRGDEKKPMLQRIYGTAWENEQQLKAYLHFKEEAKRRDHRRLGQDLDLFSIQNDAGG 300

Query: 301 GLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQM 360
           GLVFWHPKGAVVR++IEDLW+K+H ERGYD+LYTPHVAKA+LW+ SGH DFY ENMY+QM
Sbjct: 301 GLVFWHPKGAVVRNVIEDLWRKIHIERGYDLLYTPHVAKADLWQISGHLDFYRENMYDQM 360

Query: 361 DVENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFT 420
           ++E+ELYQLRPMNCPYHIL YKR+L SYQDFPIRVAELGTVYRYELSGSL GLFRVRGFT
Sbjct: 361 NIEDELYQLRPMNCPYHILIYKRKLHSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFT 420

Query: 421 QDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSA 480
           QDDAHIFCL DQIKDEIRGVLDLTE++LLQFGF+++EVNLSTRP KSVG+D+IWEKATSA
Sbjct: 421 QDDAHIFCLSDQIKDEIRGVLDLTEEMLLQFGFSKYEVNLSTRPEKSVGDDDIWEKATSA 480

Query: 481 LKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ--------------VD 540
           LKDAL DKGW YQIDEGGGAFYGPKID+KIEDALGRKWQCSTIQ              VD
Sbjct: 481 LKDALDDKGWSYQIDEGGGAFYGPKIDLKIEDALGRKWQCSTIQAIPRLIFSRFYGKRVD 540

Query: 541 FNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLP 600
           FNLPQRFDITYVDS SEKKRPIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSPIQ RVLP
Sbjct: 541 FNLPQRFDITYVDSTSEKKRPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPIQARVLP 600

Query: 601 VTDAQLSYCKEVSEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVT 660
           VTD QL YC EV++KL+A+G+RAEVCHGERLPKLIRNAETQKIPLMAVVGAKE+E  TVT
Sbjct: 601 VTDTQLEYCNEVAKKLKANGIRAEVCHGERLPKLIRNAETQKIPLMAVVGAKELETGTVT 660

Query: 661 VRSRFGGEIGTIAVDDFVGRIKFASENRTPF 673
           VRSRFGG++GT+ VDDFVGR K A ENRT F
Sbjct: 661 VRSRFGGDVGTMTVDDFVGRTKSAIENRTSF 684

BLAST of Cucsa.100510 vs. NCBI nr
Match: gi|470114485|ref|XP_004293445.1| (PREDICTED: probable threonine--tRNA ligase, cytoplasmic [Fragaria vesca subsp. vesca])

HSP 1 Score: 1077.0 bits (2784), Expect = 0.0e+00
Identity = 526/675 (77.93%), Postives = 592/675 (87.70%), Query Frame = 1

Query: 1   MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS 60
           M +LQRMATT+  LL SPL+ P S      R     ++ F     R F      NG STS
Sbjct: 1   MLILQRMATTSSSLLSSPLLKPSSFLSPLKRCVSQPTSDF-----RAFA--FSRNGLSTS 60

Query: 61  ISVATDSSAPSISTQNEDLVDAHKDQTEK---IVLPTNESSEKLLRIRHTCAHVMAMAVQ 120
            + A  ++ P +STQ++ L +A KD+++K   +VLP+NESSEKLLRIRHTCAHVMAMAVQ
Sbjct: 61  -AAAVAATEPHVSTQHDKLKEAQKDKSDKLDRVVLPSNESSEKLLRIRHTCAHVMAMAVQ 120

Query: 121 KLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQ 180
           KL+PDAKVTIGPWIENGFYYDFDMEPL DK+LK+IKKEM+RII RNLPLVREEVSR+EAQ
Sbjct: 121 KLYPDAKVTIGPWIENGFYYDFDMEPLTDKELKRIKKEMDRIICRNLPLVREEVSREEAQ 180

Query: 181 RRIEAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGA 240
           RRI  +NEPYK+E+L+SIK+DPITIYHIGNEWWDLCAGPHVE TGKIN++AVELE++AGA
Sbjct: 181 RRISTLNEPYKLEILDSIKDDPITIYHIGNEWWDLCAGPHVEYTGKINRKAVELESIAGA 240

Query: 241 YWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGL 300
           YWRGDEK PMLQRIYGTAWE EEQL+AYLHFKEEAKRRDHRRLG+ LDLFSIQ+DAGGGL
Sbjct: 241 YWRGDEKKPMLQRIYGTAWENEEQLQAYLHFKEEAKRRDHRRLGQVLDLFSIQNDAGGGL 300

Query: 301 VFWHPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDV 360
           VFWHPKGA+VR +IEDLWKK H ERGYD+LYTPHVAKA+LW+ SGH DFY ENMYNQMDV
Sbjct: 301 VFWHPKGAIVRHVIEDLWKKTHIERGYDLLYTPHVAKADLWQISGHLDFYRENMYNQMDV 360

Query: 361 ENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQD 420
           E+ELYQLRPMNCPYHIL YKRR  SY DFPIRVAELGTVYRYELSGSL GLFRVRGFTQD
Sbjct: 361 EDELYQLRPMNCPYHILIYKRRRHSYNDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQD 420

Query: 421 DAHIFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALK 480
           DAHIFCL+DQIKDEIRGVLDLTE+LLL+FGF+++EVNLSTRP KSVG+D+IWEKATSAL+
Sbjct: 421 DAHIFCLEDQIKDEIRGVLDLTEELLLKFGFSKYEVNLSTRPEKSVGDDDIWEKATSALR 480

Query: 481 DALGDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNS 540
           DAL DKGW YQID+GGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQRFDITY+DSNS
Sbjct: 481 DALEDKGWSYQIDDGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQRFDITYIDSNS 540

Query: 541 EKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKL 600
           EKKRPIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSP+Q  VLPVTD QL YCKE++ KL
Sbjct: 541 EKKRPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPVQAHVLPVTDTQLDYCKELTNKL 600

Query: 601 RASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDD 660
           + SG+R E+CHGERLPKLIRN+E  KIPLMAVVGAKEVE  TVTVRSR G + GT+++DD
Sbjct: 601 KVSGIRGELCHGERLPKLIRNSEMLKIPLMAVVGAKEVESGTVTVRSRSGDDPGTMSIDD 660

Query: 661 FVGRIKFASENRTPF 673
           FV RIK A E+RT F
Sbjct: 661 FVSRIKSAIESRTSF 667

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SYTM2_ARATH5.1e-30877.26Threonine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana GN... [more]
SYT_SYNPW1.8e-22062.20Threonine--tRNA ligase OS=Synechococcus sp. (strain WH7803) GN=thrS PE=3 SV=1[more]
SYT_SYNS33.1e-22062.03Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9311) GN=thrS PE=3 SV=1[more]
SYT_SYNS95.3e-22061.99Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9902) GN=thrS PE=3 SV=1[more]
SYT_SYNSC8.4e-21861.00Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9605) GN=thrS PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K179_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_7G033430 PE=3 SV=1[more]
W9SJL8_9ROSA0.0e+0078.44Threonine--tRNA ligase OS=Morus notabilis GN=L484_020038 PE=3 SV=1[more]
A0A067K3R0_JATCU3.6e-31082.69Uncharacterized protein OS=Jatropha curcas GN=JCGZ_13804 PE=3 SV=1[more]
M5WYT6_PRUPE1.1e-30977.06Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002440mg PE=3 SV=1[more]
B9IAN8_POPTR1.4e-30775.73Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s16030g PE=3 SV=2[more]
Match NameE-valueIdentityDescription
AT2G04842.12.9e-30977.26 threonyl-tRNA synthetase, putative / threonine--tRNA ligase, putativ... [more]
AT5G26830.14.3e-11138.94 Threonyl-tRNA synthetase[more]
AT1G17960.14.1e-6144.25 Threonyl-tRNA synthetase[more]
AT1G18130.14.4e-1537.60 Class II aaRS and biotin synthetases superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449453584|ref|XP_004144536.1|0.0e+00100.00PREDICTED: probable threonine--tRNA ligase, cytoplasmic [Cucumis sativus][more]
gi|659124339|ref|XP_008462105.1|0.0e+0097.62PREDICTED: probable threonine--tRNA ligase, cytoplasmic [Cucumis melo][more]
gi|1009128110|ref|XP_015881052.1|0.0e+0079.76PREDICTED: threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Ziziphus jujub... [more]
gi|703161498|ref|XP_010112806.1|0.0e+0078.44Threonine--tRNA ligase [Morus notabilis][more]
gi|470114485|ref|XP_004293445.1|0.0e+0077.93PREDICTED: probable threonine--tRNA ligase, cytoplasmic [Fragaria vesca subsp. v... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002314aa-tRNA-synt_IIb
IPR002320Thr-tRNA-ligase_IIa
IPR004154Anticodon-bd
IPR006195aa-tRNA-synth_II
IPR012947tRNA_SAD
IPR018163Thr/Ala-tRNA-synth_IIc_edit
IPR002314aa-tRNA-synt_IIb
IPR002320Thr-tRNA-ligase_IIa
IPR006195aa-tRNA-synth_II
IPR012947tRNA_SAD
IPR018163Thr/Ala-tRNA-synth_IIc_edit
IPR002314aa-tRNA-synt_IIb
IPR002320Thr-tRNA-ligase_IIa
IPR004154Anticodon-bd
IPR006195aa-tRNA-synth_II
IPR012947tRNA_SAD
IPR018163Thr/Ala-tRNA-synth_IIc_edit
Vocabulary: Molecular Function
TermDefinition
GO:0000166nucleotide binding
GO:0004812aminoacyl-tRNA ligase activity
GO:0005524ATP binding
GO:0004829threonine-tRNA ligase activity
GO:0016876ligase activity, forming aminoacyl-tRNA and related compounds
GO:0000166nucleotide binding
GO:0004812aminoacyl-tRNA ligase activity
GO:0005524ATP binding
GO:0004829threonine-tRNA ligase activity
GO:0016876ligase activity, forming aminoacyl-tRNA and related compounds
GO:0000166nucleotide binding
GO:0004812aminoacyl-tRNA ligase activity
GO:0005524ATP binding
GO:0004829threonine-tRNA ligase activity
GO:0016876ligase activity, forming aminoacyl-tRNA and related compounds
Vocabulary: Biological Process
TermDefinition
GO:0006418tRNA aminoacylation for protein translation
GO:0006435threonyl-tRNA aminoacylation
GO:0043039tRNA aminoacylation
GO:0006418tRNA aminoacylation for protein translation
GO:0006435threonyl-tRNA aminoacylation
GO:0043039tRNA aminoacylation
GO:0006418tRNA aminoacylation for protein translation
GO:0006435threonyl-tRNA aminoacylation
GO:0043039tRNA aminoacylation
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
GO:0005737cytoplasm
GO:0005737cytoplasm
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071555 cell wall organization
biological_process GO:0043039 tRNA aminoacylation
biological_process GO:0006544 glycine metabolic process
biological_process GO:0006563 L-serine metabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0006566 threonine metabolic process
biological_process GO:0006435 threonyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
biological_process GO:0009793 embryo development ending in seed dormancy
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005576 extracellular region
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0004650 polygalacturonase activity
molecular_function GO:0004829 threonine-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0016876 ligase activity, forming aminoacyl-tRNA and related compounds
molecular_function GO:0000166 nucleotide binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.100510.1Cucsa.100510.1mRNA
Cucsa.100510.2Cucsa.100510.2mRNA
Cucsa.100510.3Cucsa.100510.3mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002314Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)PFAMPF00587tRNA-synt_2bcoord: 168..376
score: 4.7
IPR002320Threonine-tRNA ligase, class IIaPRINTSPR01047TRNASYNTHTHRcoord: 354..367
score: 7.2E-52coord: 381..393
score: 7.2E-52coord: 209..232
score: 7.2E-52coord: 176..204
score: 7.2E-52coord: 311..339
score: 7.2
IPR002320Threonine-tRNA ligase, class IIaHAMAPMF_00184Thr_tRNA_synthcoord: 2..480
score: 27
IPR002320Threonine-tRNA ligase, class IIaTIGRFAMsTIGR00418TIGR00418coord: 2..475
score: 5.1E
IPR004154Anticodon-bindingGENE3DG3DSA:3.40.50.800coord: 378..480
score: 1.2
IPR004154Anticodon-bindingPFAMPF03129HGTP_anticodoncoord: 388..475
score: 1.1
IPR004154Anticodon-bindingunknownSSF52954Class II aaRS ABD-relatedcoord: 374..475
score: 3.93
IPR006195Aminoacyl-tRNA synthetase, class IIPROFILEPS50862AA_TRNA_LIGASE_IIcoord: 112..381
score: 25
IPR012947Threonyl/alanyl tRNA synthetase, SADPFAMPF07973tRNA_SADcoord: 12..64
score: 1.3
IPR012947Threonyl/alanyl tRNA synthetase, SADSMARTSM00863tRNA_SAD_4coord: 12..64
score: 2.4
IPR018163Threonyl/alanyl tRNA synthetase, class II-like, putative editing domainunknownSSF55186ThrRS/AlaRS common domaincoord: 2..86
score: 3.27
NoneNo IPR availableGENE3DG3DSA:3.30.54.20coord: 1..30
score: 2.
NoneNo IPR availableGENE3DG3DSA:3.30.930.10coord: 87..377
score: 7.5E
NoneNo IPR availableGENE3DG3DSA:3.30.980.10coord: 31..69
score: 2.7
NoneNo IPR availablePANTHERPTHR11451TRNA SYNTHETASE-RELATEDcoord: 1..481
score:
NoneNo IPR availablePANTHERPTHR11451:SF32THREONYL-TRNA SYNTHETASE / THREONINE--TRNA LIGASE-RELATEDcoord: 1..481
score:
NoneNo IPR availableunknownSSF55681Class II aaRS and biotin synthetasescoord: 88..373
score: 1.99