BLAST of Cucsa.100510 vs. Swiss-Prot
Match:
SYTM2_ARATH (Threonine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana GN=EMB2761 PE=2 SV=1)
HSP 1 Score: 1057.7 bits (2734), Expect = 5.1e-308
Identity = 513/664 (77.26%), Postives = 585/664 (88.10%), Query Frame = 1
Query: 7 MATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTSISVATD 66
MA++ LL S ++ S S+ R F+ + T R F PR + +GFST +VAT+
Sbjct: 1 MASSHSLLFSSSFLSKPSSFTSSLRRFVYLPT-------RQFWPRQR-HGFSTVFAVATE 60
Query: 67 SSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVT 126
P+IS+ K + +VLP+NESS+KLL+IRHTCAHVMAMAVQKLFPDAKVT
Sbjct: 61 ---PAISSSGPK-----KAEPSTVVLPSNESSDKLLKIRHTCAHVMAMAVQKLFPDAKVT 120
Query: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 186
IGPWI+NGFYYDFDMEPL DKDLK+IKKEM+RIISRNLPL+REEVSR+EA++RI AINEP
Sbjct: 121 IGPWIDNGFYYDFDMEPLTDKDLKRIKKEMDRIISRNLPLLREEVSREEAKKRIMAINEP 180
Query: 187 YKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246
YKME+L+ IKE+PIT+YHIGNEWWDLCAGPHVE+TGKINK+AVELE+VAGAYWRGDEK
Sbjct: 181 YKMEILDGIKEEPITVYHIGNEWWDLCAGPHVETTGKINKKAVELESVAGAYWRGDEKRQ 240
Query: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 306
MLQRIYGTAWE+EEQLKAYLHFKEEAKRRDHRR+G+DLDLFSIQD+AGGGLVFWHPKGA+
Sbjct: 241 MLQRIYGTAWESEEQLKAYLHFKEEAKRRDHRRIGQDLDLFSIQDEAGGGLVFWHPKGAI 300
Query: 307 VRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRP 366
VR+IIE+ WKK+H E GYD++YTPHVAKA+LWK SGH DFY ENMY+QM++E+ELYQLRP
Sbjct: 301 VRNIIEESWKKMHVEHGYDLIYTPHVAKADLWKISGHLDFYRENMYDQMEIEDELYQLRP 360
Query: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDD 426
MNCPYHIL Y+R+ SY+D PIRVAELGTVYRYELSGSL GLFRVRGFTQDDAHIFCL+D
Sbjct: 361 MNCPYHILLYQRKRQSYRDLPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLED 420
Query: 427 QIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDALGDKGWG 486
QIKDEIRGVLDLTE++L +FGFN++EVNLSTRP KSVG D+IWEKAT AL+DAL DKGW
Sbjct: 421 QIKDEIRGVLDLTEEILSRFGFNKYEVNLSTRPEKSVGGDDIWEKATCALRDALDDKGWS 480
Query: 487 YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIH 546
Y++DEGGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQRFDITYVD+NS+KKRPIMIH
Sbjct: 481 YEVDEGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQRFDITYVDTNSDKKRPIMIH 540
Query: 547 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRASGVRAEV 606
RAVLGSLERFFG+LIE+YAGDFPLWLSP+QVRVLPVTD QL +CKEVS+KLRA GVRAE+
Sbjct: 541 RAVLGSLERFFGVLIEHYAGDFPLWLSPVQVRVLPVTDNQLEFCKEVSKKLRACGVRAEL 600
Query: 607 CHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVGRIKFAS 666
CHGERLPKLIRNAETQKIPLMAVVG KEVE TVTVRSRFGGE+GTI VDD + +I A
Sbjct: 601 CHGERLPKLIRNAETQKIPLMAVVGPKEVETGTVTVRSRFGGELGTIPVDDLINKINIAV 648
Query: 667 ENRT 671
E RT
Sbjct: 661 ETRT 648
BLAST of Cucsa.100510 vs. Swiss-Prot
Match:
SYT_SYNPW (Threonine--tRNA ligase OS=Synechococcus sp. (strain WH7803) GN=thrS PE=3 SV=1)
HSP 1 Score: 766.9 bits (1979), Expect = 1.8e-220
Identity = 362/582 (62.20%), Postives = 457/582 (78.52%), Query Frame = 1
Query: 90 IVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDM-EPLGDKD 149
+VLP +E+LLRIRH+ +HVMAMAVQ+LFP+A+VTIGPW E+GFYYDFD EP + D
Sbjct: 23 VVLPKTSENEQLLRIRHSMSHVMAMAVQQLFPNARVTIGPWTESGFYYDFDNPEPFTEAD 82
Query: 150 LKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMELLESIKEDPITIYHIGNE 209
LK IKK M +II++ LPL R EVSR EA+ +I+A NEPYK+E+LE ++E PIT+Y +G +
Sbjct: 83 LKAIKKGMIKIINKKLPLERVEVSRSEAEEKIKAQNEPYKLEILEGLQE-PITLYTLGED 142
Query: 210 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 269
WWDLCAGPHVE+TG++N +A ELE+VAGAYWRGDE LQRIYGTAWET EQL +
Sbjct: 143 WWDLCAGPHVENTGQLNAKAFELESVAGAYWRGDETKAQLQRIYGTAWETAEQLAEHKRR 202
Query: 270 KEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLY 329
KEEA RRDHRR+GKDLDLFSI+D+AG GLVFWHP+GA +R +IE+ W++ H E GY++LY
Sbjct: 203 KEEALRRDHRRIGKDLDLFSIEDEAGAGLVFWHPRGARMRLLIEEFWRQAHFEGGYELLY 262
Query: 330 TPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPI 389
TPHVA LWKTSGH DFY E+M+ M+V+ YQL+PMNCP+H+L Y +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMEVDEREYQLKPMNCPFHVLTYASKLRSYRELPI 322
Query: 390 RVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGF 449
R AELGTVYRYE G + GL RVRGFTQDDAH+FCL +QI DEI +LDLTE +L F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPEQISDEILKILDLTERILSTFDF 382
Query: 450 NEFEVNLSTRPPKSVGNDEIWEKATSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDA 509
N +E+NLSTRP KS+G+D +W+ AT L +AL KGW Y+IDEGGGAFYGPKID+KIEDA
Sbjct: 383 NTYEINLSTRPEKSIGDDAVWDLATKGLIEALERKGWAYKIDEGGGAFYGPKIDLKIEDA 442
Query: 510 LGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDF 569
+GR WQCSTIQ+DFNLP+RF + Y+ ++ K+RPIMIHRA+ GSLERFFGI+ ENYAGD+
Sbjct: 443 IGRMWQCSTIQLDFNLPERFKLDYIAADGSKQRPIMIHRAIFGSLERFFGIMTENYAGDY 502
Query: 570 PLWLSPIQVRVLPVTDAQLSYCKEVSEKLRASGVRAEVCH-GERLPKLIRNAETQKIPLM 629
PLWL+P QVR+LPVTD Y + + ++L +GVRA + G+RL KLIR E KIP++
Sbjct: 503 PLWLAPEQVRLLPVTDEVQPYAESLLDQLTQAGVRATIDRSGDRLGKLIRTGEQMKIPVL 562
Query: 630 AVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVGRIKFASENR 670
AV+GAKE E V++RSR G++G +AV D + + A+ R
Sbjct: 563 AVIGAKEAEQNAVSLRSRRDGDLGVVAVADLLRAAQNANSQR 603
BLAST of Cucsa.100510 vs. Swiss-Prot
Match:
SYT_SYNS3 (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9311) GN=thrS PE=3 SV=1)
HSP 1 Score: 766.1 bits (1977), Expect = 3.1e-220
Identity = 361/582 (62.03%), Postives = 454/582 (78.01%), Query Frame = 1
Query: 90 IVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDM-EPLGDKD 149
+VLP SE LL+IRH+ +HVMAMAVQKLFP A+VTIGPW E GFYYDFD EP + D
Sbjct: 23 VVLPKTSESENLLKIRHSMSHVMAMAVQKLFPKAQVTIGPWTEAGFYYDFDNPEPFTEAD 82
Query: 150 LKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMELLESIKEDPITIYHIGNE 209
LK IKKEM +II R LPL R EVSR+EA+RRI+A NEPYK+E+LE + E PIT+Y +G +
Sbjct: 83 LKAIKKEMGKIIGRKLPLERIEVSREEAERRIKAQNEPYKLEILERLVE-PITLYTLGEQ 142
Query: 210 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 269
WWDLCAGPHV +T ++N +A ELE+VAGAYWRGDE LQRIYGTAWET +QL +
Sbjct: 143 WWDLCAGPHVANTSELNPKAFELESVAGAYWRGDETKAQLQRIYGTAWETADQLSEHKRR 202
Query: 270 KEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLY 329
KEEA RRDHRRLGKDLDLFSI+D+AG GLVFWHP+GA +R +IED W++ H E GY++LY
Sbjct: 203 KEEALRRDHRRLGKDLDLFSIEDEAGAGLVFWHPRGARMRLLIEDFWRQAHFEGGYELLY 262
Query: 330 TPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPI 389
TPHVA LWKTSGH DFY E+M+ M+V+ YQL+PMNCP+H+L Y +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMEVDERQYQLKPMNCPFHVLTYASKLRSYRELPI 322
Query: 390 RVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGF 449
R AELGTVYRYE G + GL RVRGFTQDDAH+FCL +QI DEI +L+LTE +L F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPEQISDEILRILNLTERILSTFDF 382
Query: 450 NEFEVNLSTRPPKSVGNDEIWEKATSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDA 509
+ +E+NLST+P K++G+D +WE AT L +AL KGW Y+IDEGGGAFYGPKID+KIEDA
Sbjct: 383 SNYEINLSTKPDKAIGDDAVWELATKGLIEALKRKGWAYKIDEGGGAFYGPKIDLKIEDA 442
Query: 510 LGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDF 569
+GR WQCSTIQ+DFNLP+RF++ Y+ ++ K+RPIMIHRA+ GSLERFFGI+ ENYAGDF
Sbjct: 443 IGRMWQCSTIQLDFNLPERFELDYIAADGSKQRPIMIHRAIFGSLERFFGIMTENYAGDF 502
Query: 570 PLWLSPIQVRVLPVTDAQLSYCKEVSEKLRASGVRAEVCH-GERLPKLIRNAETQKIPLM 629
P WL+P Q+R+LPVTD L Y +E +L+A+G+RA + G+RL KLIR E KIP++
Sbjct: 503 PFWLAPEQIRLLPVTDEVLGYAEEFQNQLKAAGIRASIDRSGDRLGKLIRIGEKMKIPVL 562
Query: 630 AVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVGRIKFASENR 670
AV+GAKE E ++RSR G++G I + + + A+++R
Sbjct: 563 AVIGAKEAEQGAASLRSRRDGDLGVITKERLIATAQSANQDR 603
BLAST of Cucsa.100510 vs. Swiss-Prot
Match:
SYT_SYNS9 (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9902) GN=thrS PE=3 SV=1)
HSP 1 Score: 765.4 bits (1975), Expect = 5.3e-220
Identity = 362/584 (61.99%), Postives = 455/584 (77.91%), Query Frame = 1
Query: 90 IVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDM-EPLGDKD 149
+VLP SE+LL+IRH+ +HVMAMAVQKLFP+A+VTIGPW E GFYYDFD EP + D
Sbjct: 23 VVLPKTSESEQLLKIRHSMSHVMAMAVQKLFPNAQVTIGPWTETGFYYDFDNPEPFTEDD 82
Query: 150 LKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMELLESIKEDPITIYHIGNE 209
LK IKKEM +II R LPL R EVSRDEA+ RI+A NEPYK+E+LE ++E PIT+Y +G++
Sbjct: 83 LKAIKKEMGKIIGRKLPLERIEVSRDEAETRIKAQNEPYKLEILERLQE-PITLYTLGDQ 142
Query: 210 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 269
WWDLCAGPHVE+T +++ +A ELE+VAGAYWRGDE LQRIYGTAWET EQL +
Sbjct: 143 WWDLCAGPHVENTKELHPKAFELESVAGAYWRGDETKAQLQRIYGTAWETPEQLAEHKRR 202
Query: 270 KEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLY 329
K EA RRDHRRLGKDL+LFSI+D+AG GLVFWHP+GA +R +IED W++ H E GY++LY
Sbjct: 203 KVEALRRDHRRLGKDLELFSIEDEAGAGLVFWHPRGARMRLLIEDFWRQAHFEGGYELLY 262
Query: 330 TPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPI 389
TPHVA LWKTSGH DFY E+M+ M V+ YQL+PMNCP+H+L Y +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMQVDEREYQLKPMNCPFHVLTYASKLRSYRELPI 322
Query: 390 RVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGF 449
R AELGTVYRYE G + GL RVRGFTQDDAH+FCL DQI DEI +LDLTE +L F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPDQISDEILRILDLTERILSTFDF 382
Query: 450 NEFEVNLSTRPPKSVGNDEIWEKATSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDA 509
N +E+NLSTRP KS+G++ +WE AT L +AL KGW ++IDEGGGAFYGPKID+KIEDA
Sbjct: 383 NHYEINLSTRPEKSIGSEAVWELATKGLTEALERKGWNFKIDEGGGAFYGPKIDLKIEDA 442
Query: 510 LGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDF 569
+GR WQCSTIQ+DFNLP+RF + YV ++ K++PIMIHRA+ GSLERFFGI+ ENYAGDF
Sbjct: 443 IGRMWQCSTIQLDFNLPERFGLDYVAADGSKQQPIMIHRAIFGSLERFFGIMTENYAGDF 502
Query: 570 PLWLSPIQVRVLPVTDAQLSYCKEVSEKLRASGVRAEVCH-GERLPKLIRNAETQKIPLM 629
P WL+P Q+R+LPVTD Y ++V +L+ +G+RA + G+RL K+IR E KIP++
Sbjct: 503 PFWLAPEQIRLLPVTDDVQPYAEQVLNQLKTAGIRATIDQSGDRLGKIIRTGEQMKIPVL 562
Query: 630 AVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVGRIKFASENRTP 672
AV+GAKE E +++RSR G++G +VD + + A+ R P
Sbjct: 563 AVIGAKEAEQNAISLRSRRDGDLGVTSVDALLKAAQRANAERQP 605
BLAST of Cucsa.100510 vs. Swiss-Prot
Match:
SYT_SYNSC (Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9605) GN=thrS PE=3 SV=1)
HSP 1 Score: 758.1 bits (1956), Expect = 8.4e-218
Identity = 355/582 (61.00%), Postives = 453/582 (77.84%), Query Frame = 1
Query: 90 IVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDM-EPLGDKD 149
+VLP S++LL+IRH+ +HVMAMAVQ+LFP A+VTIGPW E GFYYDFD +P + D
Sbjct: 23 VVLPKTSESDQLLKIRHSMSHVMAMAVQQLFPKARVTIGPWTETGFYYDFDNPDPFTEAD 82
Query: 150 LKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEPYKMELLESIKEDPITIYHIGNE 209
LK IKK M +II++ LPL R EVSR+EA+ +I+A NEPYK+E+L+ + E PIT+Y +G +
Sbjct: 83 LKAIKKGMIKIINKKLPLQRVEVSRNEAEEKIKAQNEPYKLEILQGLHE-PITLYTLGED 142
Query: 210 WWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHF 269
WWDLCAGPHV+ TG++N +A ELE++AGAYWRGDE LQRIYGTAWE+ EQL +
Sbjct: 143 WWDLCAGPHVDHTGQLNAKAFELESLAGAYWRGDETKAQLQRIYGTAWESPEQLAEHKRR 202
Query: 270 KEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLY 329
KEEA RRDHRR+GKDLDLFSI+D+AG GLVFWHP+GA +R +IE+ W++ H E GY++LY
Sbjct: 203 KEEALRRDHRRIGKDLDLFSIEDEAGAGLVFWHPRGARIRLLIEEFWRQAHFEGGYELLY 262
Query: 330 TPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPI 389
TPHVA LWKTSGH DFY E+M+ M+V+ YQL+PMNCP+H+L Y +L SY++ PI
Sbjct: 263 TPHVADISLWKTSGHLDFYAESMFGPMEVDEREYQLKPMNCPFHVLTYASKLRSYRELPI 322
Query: 390 RVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGF 449
R AELGTVYRYE G + GL RVRGFTQDDAH+FCL +QI DEI +LDLTE +L F F
Sbjct: 323 RWAELGTVYRYERPGVMHGLMRVRGFTQDDAHVFCLPEQISDEILKILDLTERILSAFDF 382
Query: 450 NEFEVNLSTRPPKSVGNDEIWEKATSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDA 509
+ +E+NLSTRP KS+G D +W+ AT L +AL KGW Y+IDEGGGAFYGPKID+KIEDA
Sbjct: 383 SNYEINLSTRPEKSIGEDAVWDLATKGLIEALERKGWAYKIDEGGGAFYGPKIDLKIEDA 442
Query: 510 LGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDF 569
+GR WQCSTIQ+DFNLP+RF++ Y+ ++ K+RPIMIHRA+ GSLERFFGI+ ENYAGD+
Sbjct: 443 IGRMWQCSTIQLDFNLPERFELDYIAADGSKQRPIMIHRAIFGSLERFFGIMTENYAGDY 502
Query: 570 PLWLSPIQVRVLPVTDAQLSYCKEVSEKLRASGVRAEVCH-GERLPKLIRNAETQKIPLM 629
P WL+P QVR+LPVTD Y +++ ++L +GVRA V G+RL KLIR E KIP++
Sbjct: 503 PFWLAPEQVRLLPVTDEVQPYAEQLLDQLTKAGVRATVDRSGDRLGKLIRTGEQMKIPVL 562
Query: 630 AVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVGRIKFASENR 670
AV+GAKE E V++RSR G++G AV D + + A+ R
Sbjct: 563 AVIGAKEAEQNAVSLRSRRDGDLGVTAVSDLLSAAQMANSER 603
BLAST of Cucsa.100510 vs. TrEMBL
Match:
A0A0A0K179_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G033430 PE=3 SV=1)
HSP 1 Score: 1365.9 bits (3534), Expect = 0.0e+00
Identity = 672/672 (100.00%), Postives = 672/672 (100.00%), Query Frame = 1
Query: 1 MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS 60
MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS
Sbjct: 1 MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS 60
Query: 61 ISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
ISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61 ISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
Query: 181 EAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
EAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
Query: 301 HPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
HPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
Query: 421 IFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480
IFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480
Query: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK
Sbjct: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
Query: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS 600
RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS
Sbjct: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS 600
Query: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVG 660
GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVG
Sbjct: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVG 660
Query: 661 RIKFASENRTPF 673
RIKFASENRTPF
Sbjct: 661 RIKFASENRTPF 672
BLAST of Cucsa.100510 vs. TrEMBL
Match:
W9SJL8_9ROSA (Threonine--tRNA ligase OS=Morus notabilis GN=L484_020038 PE=3 SV=1)
HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 542/691 (78.44%), Postives = 605/691 (87.55%), Query Frame = 1
Query: 1 MFVLQRMATTTGLLL-HSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFST 60
MF+L RMAT+ L S + P SP+ S R L ++ +F R F NGFST
Sbjct: 1 MFILHRMATSQSLFAPSSSFLKPSSPS-SFNRLGLPLTRQF-----RAFALHGHRNGFST 60
Query: 61 SIS-VATDSSAPSISTQNEDLV---DAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMA 120
+ S VAT+SSA + S +N +L D + + E++VLPTN+SSEKLLRIRHTCAHVMAMA
Sbjct: 61 AYSAVATESSAET-SVENLELETNGDENSQKEERVVLPTNDSSEKLLRIRHTCAHVMAMA 120
Query: 121 VQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDE 180
VQKLFPDAKVTIGPWIENGFYYDFDMEPL D+DLKKIKKEM+RI+ +NLPL+REEVSRDE
Sbjct: 121 VQKLFPDAKVTIGPWIENGFYYDFDMEPLTDRDLKKIKKEMDRIVGKNLPLIREEVSRDE 180
Query: 181 AQRRIEAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVA 240
AQRRI ++NEPYK+E+LESIKE+PITIYHIGNEWWDLCAGPHVESTGKIN+RAVELE+VA
Sbjct: 181 AQRRIMSLNEPYKLEILESIKEEPITIYHIGNEWWDLCAGPHVESTGKINRRAVELESVA 240
Query: 241 GAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGG 300
GAYWRGDEK PMLQRIYGTAWE E+QLKAYLHFKEEAKRRDHRRLG+DLDLFSIQ+DAGG
Sbjct: 241 GAYWRGDEKKPMLQRIYGTAWENEQQLKAYLHFKEEAKRRDHRRLGQDLDLFSIQNDAGG 300
Query: 301 GLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQM 360
GLVFWHPKGAVVR++IEDLW+K+H ERGYD+LYTPHVAKA+LW+ SGH DFY ENMY+QM
Sbjct: 301 GLVFWHPKGAVVRNVIEDLWRKIHIERGYDLLYTPHVAKADLWQISGHLDFYRENMYDQM 360
Query: 361 DVENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFT 420
++E+ELYQLRPMNCPYHIL YKR+L SYQDFPIRVAELGTVYRYELSGSL GLFRVRGFT
Sbjct: 361 NIEDELYQLRPMNCPYHILIYKRKLHSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFT 420
Query: 421 QDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSA 480
QDDAHIFCL DQIKDEIRGVLDLTE++LLQFGF+++EVNLSTRP KSVG+D+IWEKATSA
Sbjct: 421 QDDAHIFCLSDQIKDEIRGVLDLTEEMLLQFGFSKYEVNLSTRPEKSVGDDDIWEKATSA 480
Query: 481 LKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ--------------VD 540
LKDAL DKGW YQIDEGGGAFYGPKID+KIEDALGRKWQCSTIQ VD
Sbjct: 481 LKDALDDKGWSYQIDEGGGAFYGPKIDLKIEDALGRKWQCSTIQAIPRLIFSRFYGKRVD 540
Query: 541 FNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLP 600
FNLPQRFDITYVDS SEKKRPIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSPIQ RVLP
Sbjct: 541 FNLPQRFDITYVDSTSEKKRPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPIQARVLP 600
Query: 601 VTDAQLSYCKEVSEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVT 660
VTD QL YC EV++KL+A+G+RAEVCHGERLPKLIRNAETQKIPLMAVVGAKE+E TVT
Sbjct: 601 VTDTQLEYCNEVAKKLKANGIRAEVCHGERLPKLIRNAETQKIPLMAVVGAKELETGTVT 660
Query: 661 VRSRFGGEIGTIAVDDFVGRIKFASENRTPF 673
VRSRFGG++GT+ VDDFVGR K A ENRT F
Sbjct: 661 VRSRFGGDVGTMTVDDFVGRTKSAIENRTSF 684
BLAST of Cucsa.100510 vs. TrEMBL
Match:
A0A067K3R0_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_13804 PE=3 SV=1)
HSP 1 Score: 1071.6 bits (2770), Expect = 3.6e-310
Identity = 511/618 (82.69%), Postives = 568/618 (91.91%), Query Frame = 1
Query: 53 KSNGFSTSISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVM 112
K NG S S +VAT+S+ + +N+ + + +++TEKIVLPTNESSEKLLRIRHTCAHVM
Sbjct: 56 KRNGLSMSTAVATESAT---AARNDVVEETQREETEKIVLPTNESSEKLLRIRHTCAHVM 115
Query: 113 AMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVS 172
AMAVQKL+P+AKVTIGPWI+NGFYYDFDMEPL DKDLKKIKKEM+RII RNLPLVREEVS
Sbjct: 116 AMAVQKLYPEAKVTIGPWIDNGFYYDFDMEPLTDKDLKKIKKEMDRIIGRNLPLVREEVS 175
Query: 173 RDEAQRRIEAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELE 232
RDEA+RRI AINEPYKME+LESIKEDPITIYHIG EWWDLCAGPHVE+TG INK++VELE
Sbjct: 176 RDEAKRRIMAINEPYKMEILESIKEDPITIYHIGAEWWDLCAGPHVETTGLINKKSVELE 235
Query: 233 TVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDD 292
+VAGAYWRGDEKNPMLQRIYGTAWE E+QLKAYLHFKEEAKRRDHRRLG+DLDLFSIQD+
Sbjct: 236 SVAGAYWRGDEKNPMLQRIYGTAWENEQQLKAYLHFKEEAKRRDHRRLGQDLDLFSIQDE 295
Query: 293 AGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMY 352
AGGGLVFWHPKGA+VR IIED WKK H + GYD+LYTPHVAKAELWK SGH DFY ENMY
Sbjct: 296 AGGGLVFWHPKGAIVRHIIEDSWKKTHIKHGYDLLYTPHVAKAELWKISGHLDFYRENMY 355
Query: 353 NQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVR 412
+QM +E+E YQLRPMNCPYHIL +KR+L SY+DFPIRVAELGTVYRYELSGSL GLFRVR
Sbjct: 356 DQMQIEDENYQLRPMNCPYHILVFKRKLNSYRDFPIRVAELGTVYRYELSGSLHGLFRVR 415
Query: 413 GFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKA 472
GFTQDDAHIFCL+DQIKDEIRGVLDLTE++LLQFGF+++EVNLSTRP K VG D+IWEKA
Sbjct: 416 GFTQDDAHIFCLEDQIKDEIRGVLDLTEEILLQFGFSKYEVNLSTRPEKFVGGDDIWEKA 475
Query: 473 TSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITY 532
TSAL+DAL DKGW YQIDEGGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQRFDITY
Sbjct: 476 TSALRDALEDKGWNYQIDEGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQRFDITY 535
Query: 533 VDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKE 592
VDSN+EKK+PIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSPIQVRVLPVTD QL YCKE
Sbjct: 536 VDSNTEKKQPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPIQVRVLPVTDTQLEYCKE 595
Query: 593 VSEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGT 652
V+ KL+ + +R EVCHGERLPKLIRNAE QKIPLMAVVGAKEVE Q+VT+RSRF GE+GT
Sbjct: 596 VTNKLKGNDIRTEVCHGERLPKLIRNAEKQKIPLMAVVGAKEVETQSVTIRSRFSGELGT 655
Query: 653 IAVDDFVGRIKFASENRT 671
+ +DDF+ RIKFA + RT
Sbjct: 656 MKIDDFISRIKFAVDKRT 670
BLAST of Cucsa.100510 vs. TrEMBL
Match:
M5WYT6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002440mg PE=3 SV=1)
HSP 1 Score: 1070.1 bits (2766), Expect = 1.1e-309
Identity = 524/680 (77.06%), Postives = 597/680 (87.79%), Query Frame = 1
Query: 1 MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFL--------AVSTKFARNRDRPFLPRN 60
M +LQRMAT++ + +PL+ S S P FL A ST+F L RN
Sbjct: 1 MLILQRMATSSSFI-STPLLKASS---SFPTHFLSPLKHCVSAPSTEFRALA----LYRN 60
Query: 61 KSNGFSTSISVATDSSAPSISTQNEDLVDAHK---DQTEKIVLPTNESSEKLLRIRHTCA 120
++ ++S +VAT++ +STQ++ L + K D+ EK+VLPTNESSE LLRIRHTCA
Sbjct: 61 RNGVSTSSAAVATETQ---VSTQDDKLKETQKEKSDKLEKVVLPTNESSESLLRIRHTCA 120
Query: 121 HVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVRE 180
HVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPL DK+LK+IKKEM+RII +NLPL+RE
Sbjct: 121 HVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLTDKELKRIKKEMDRIIGKNLPLIRE 180
Query: 181 EVSRDEAQRRIEAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAV 240
E+SRDEAQRRI A+NEPYK+E+L+SIKEDPITIYHIGNEWWDLCAGPHVE TGKIN++AV
Sbjct: 181 EISRDEAQRRITALNEPYKLEILDSIKEDPITIYHIGNEWWDLCAGPHVEYTGKINRKAV 240
Query: 241 ELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSI 300
ELE++AGAYWRGDEK PMLQRIYGTAWE+E+QLKAYLHFKEEAKRRDHRRLG+ LDLFSI
Sbjct: 241 ELESIAGAYWRGDEKKPMLQRIYGTAWESEDQLKAYLHFKEEAKRRDHRRLGQVLDLFSI 300
Query: 301 QDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGE 360
Q+DAGGGLVFWHPKGA+VR +IEDLWKK+H ERGYD+LYTPHVAKA+LW+ SGH DFY E
Sbjct: 301 QNDAGGGLVFWHPKGAIVRHVIEDLWKKIHIERGYDLLYTPHVAKADLWRISGHLDFYKE 360
Query: 361 NMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLF 420
NMY+QM++E+ELYQLRPMNCPYHIL YKRR SY DFPIRVAELGTVYRYELSGSL GLF
Sbjct: 361 NMYDQMNIEDELYQLRPMNCPYHILIYKRRPHSYHDFPIRVAELGTVYRYELSGSLHGLF 420
Query: 421 RVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIW 480
RVRGFTQDDAHIFCL+DQIKDEIRGVLDLTE+LLLQFGF+++EVNLSTRP KSVG+D+IW
Sbjct: 421 RVRGFTQDDAHIFCLEDQIKDEIRGVLDLTEELLLQFGFSKYEVNLSTRPEKSVGDDDIW 480
Query: 481 EKATSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFD 540
KATSAL+DAL DKGW YQID+GGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQRFD
Sbjct: 481 VKATSALRDALDDKGWSYQIDDGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQRFD 540
Query: 541 ITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSY 600
ITYVDSNSEKKRPIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSP+Q VLPVTD QL Y
Sbjct: 541 ITYVDSNSEKKRPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPVQAHVLPVTDTQLDY 600
Query: 601 CKEVSEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGE 660
CKEV+ KL+A+G+R E+CHGERLPKLIRN+E KIPLMAVVGAKEVE +VTVRSRFGG+
Sbjct: 601 CKEVTNKLKANGIRGELCHGERLPKLIRNSEMLKIPLMAVVGAKEVETGSVTVRSRFGGD 660
Query: 661 IGTIAVDDFVGRIKFASENR 670
+GT+A+DDFV IK A E+R
Sbjct: 661 LGTMAIDDFVSTIKSAIESR 669
BLAST of Cucsa.100510 vs. TrEMBL
Match:
B9IAN8_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s16030g PE=3 SV=2)
HSP 1 Score: 1063.1 bits (2748), Expect = 1.4e-307
Identity = 518/684 (75.73%), Postives = 580/684 (84.80%), Query Frame = 1
Query: 1 MFVLQRMATTTGLLLHS-----------PLVTPISPNVSAPRFFLAVSTKFARNRDRPFL 60
MFVL +MAT+ L S PL+ P ++ +P +ST D
Sbjct: 1 MFVLHKMATSPSSSLCSFSLFLSKPSSLPLLKPPLLSLHSPPLKRFISTASELQHDTQAR 60
Query: 61 PRNKSNGFSTSISVATDSSAPSISTQNEDLVD---AHKDQTEKIVLPTNESSEKLLRIRH 120
R T S S +I+T++ + A ++TEK+VLPTN+SSEKLLRIRH
Sbjct: 61 RRTLLGSPYTKTSPLLSLSTAAIATESATVSHNDTAENEETEKLVLPTNDSSEKLLRIRH 120
Query: 121 TCAHVMAMAVQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPL 180
TCAHVMAMAVQKL+P+AKVTIGPWIENGFYYDFDM+PL DKDLK+IKKEM+RII RNLPL
Sbjct: 121 TCAHVMAMAVQKLYPEAKVTIGPWIENGFYYDFDMDPLTDKDLKRIKKEMDRIIGRNLPL 180
Query: 181 VREEVSRDEAQRRIEAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINK 240
RE+VSRDEAQRRI A+NEPYKME+LESIKEDPITIYHIG+EWWDLCAGPHVESTG IN+
Sbjct: 181 AREDVSRDEAQRRIMAVNEPYKMEILESIKEDPITIYHIGDEWWDLCAGPHVESTGNINR 240
Query: 241 RAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDL 300
+AVELE+VAGAYWRGDEK PMLQRIYGTAWE E+QLKAYLHFKEEAKRRDHRRLG+DLDL
Sbjct: 241 KAVELESVAGAYWRGDEKKPMLQRIYGTAWENEDQLKAYLHFKEEAKRRDHRRLGQDLDL 300
Query: 301 FSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDF 360
FSIQD+AGGGLVFWHPKGAV+R IIED WKK H E GYD+LYTPHVAKA+LWK SGH DF
Sbjct: 301 FSIQDEAGGGLVFWHPKGAVIRHIIEDSWKKTHIEHGYDLLYTPHVAKADLWKISGHLDF 360
Query: 361 YGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLR 420
Y ENM++QM +E+E YQLRPMNCPYHIL YKR+L SY+DFPIRVAELGTVYRYELSGSL
Sbjct: 361 YRENMFDQMKIEDEQYQLRPMNCPYHILLYKRKLNSYRDFPIRVAELGTVYRYELSGSLH 420
Query: 421 GLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGND 480
GLFRVRGFTQDDAHIFCL+DQIKDEIRGVLDLTE++LLQFGF +EVNLSTRP KSVG+D
Sbjct: 421 GLFRVRGFTQDDAHIFCLEDQIKDEIRGVLDLTEEILLQFGFRRYEVNLSTRPEKSVGDD 480
Query: 481 EIWEKATSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQ 540
+IWEKATSAL+DAL DKGW +QIDEGGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQ
Sbjct: 481 DIWEKATSALRDALDDKGWSFQIDEGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQ 540
Query: 541 RFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQ 600
RFD+TYVDSN+EKKRPIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSP+QVR+LPVTD Q
Sbjct: 541 RFDVTYVDSNTEKKRPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPVQVRILPVTDTQ 600
Query: 601 LSYCKEVSEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRF 660
L YCKEV KL+ +G+RAEVC GERLPKLIRNAE QKIPLMAVVG KE E QTVT+RSRF
Sbjct: 601 LEYCKEVISKLKGNGIRAEVCQGERLPKLIRNAEKQKIPLMAVVGPKEAETQTVTIRSRF 660
Query: 661 GGEIGTIAVDDFVGRIKFASENRT 671
GE+GTI +DDF+ RIK A E RT
Sbjct: 661 SGEVGTIEIDDFISRIKSAIERRT 684
BLAST of Cucsa.100510 vs. TAIR10
Match:
AT2G04842.1 (AT2G04842.1 threonyl-tRNA synthetase, putative / threonine--tRNA ligase, putative)
HSP 1 Score: 1057.7 bits (2734), Expect = 2.9e-309
Identity = 513/664 (77.26%), Postives = 585/664 (88.10%), Query Frame = 1
Query: 7 MATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTSISVATD 66
MA++ LL S ++ S S+ R F+ + T R F PR + +GFST +VAT+
Sbjct: 1 MASSHSLLFSSSFLSKPSSFTSSLRRFVYLPT-------RQFWPRQR-HGFSTVFAVATE 60
Query: 67 SSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLFPDAKVT 126
P+IS+ K + +VLP+NESS+KLL+IRHTCAHVMAMAVQKLFPDAKVT
Sbjct: 61 ---PAISSSGPK-----KAEPSTVVLPSNESSDKLLKIRHTCAHVMAMAVQKLFPDAKVT 120
Query: 127 IGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRIEAINEP 186
IGPWI+NGFYYDFDMEPL DKDLK+IKKEM+RIISRNLPL+REEVSR+EA++RI AINEP
Sbjct: 121 IGPWIDNGFYYDFDMEPLTDKDLKRIKKEMDRIISRNLPLLREEVSREEAKKRIMAINEP 180
Query: 187 YKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWRGDEKNP 246
YKME+L+ IKE+PIT+YHIGNEWWDLCAGPHVE+TGKINK+AVELE+VAGAYWRGDEK
Sbjct: 181 YKMEILDGIKEEPITVYHIGNEWWDLCAGPHVETTGKINKKAVELESVAGAYWRGDEKRQ 240
Query: 247 MLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFWHPKGAV 306
MLQRIYGTAWE+EEQLKAYLHFKEEAKRRDHRR+G+DLDLFSIQD+AGGGLVFWHPKGA+
Sbjct: 241 MLQRIYGTAWESEEQLKAYLHFKEEAKRRDHRRIGQDLDLFSIQDEAGGGLVFWHPKGAI 300
Query: 307 VRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENELYQLRP 366
VR+IIE+ WKK+H E GYD++YTPHVAKA+LWK SGH DFY ENMY+QM++E+ELYQLRP
Sbjct: 301 VRNIIEESWKKMHVEHGYDLIYTPHVAKADLWKISGHLDFYRENMYDQMEIEDELYQLRP 360
Query: 367 MNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDD 426
MNCPYHIL Y+R+ SY+D PIRVAELGTVYRYELSGSL GLFRVRGFTQDDAHIFCL+D
Sbjct: 361 MNCPYHILLYQRKRQSYRDLPIRVAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFCLED 420
Query: 427 QIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDALGDKGWG 486
QIKDEIRGVLDLTE++L +FGFN++EVNLSTRP KSVG D+IWEKAT AL+DAL DKGW
Sbjct: 421 QIKDEIRGVLDLTEEILSRFGFNKYEVNLSTRPEKSVGGDDIWEKATCALRDALDDKGWS 480
Query: 487 YQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIH 546
Y++DEGGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQRFDITYVD+NS+KKRPIMIH
Sbjct: 481 YEVDEGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQRFDITYVDTNSDKKRPIMIH 540
Query: 547 RAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRASGVRAEV 606
RAVLGSLERFFG+LIE+YAGDFPLWLSP+QVRVLPVTD QL +CKEVS+KLRA GVRAE+
Sbjct: 541 RAVLGSLERFFGVLIEHYAGDFPLWLSPVQVRVLPVTDNQLEFCKEVSKKLRACGVRAEL 600
Query: 607 CHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVGRIKFAS 666
CHGERLPKLIRNAETQKIPLMAVVG KEVE TVTVRSRFGGE+GTI VDD + +I A
Sbjct: 601 CHGERLPKLIRNAETQKIPLMAVVGPKEVETGTVTVRSRFGGELGTIPVDDLINKINIAV 648
Query: 667 ENRT 671
E RT
Sbjct: 661 ETRT 648
BLAST of Cucsa.100510 vs. TAIR10
Match:
AT5G26830.1 (AT5G26830.1 Threonyl-tRNA synthetase)
HSP 1 Score: 399.4 bits (1025), Expect = 4.3e-111
Identity = 220/565 (38.94%), Postives = 338/565 (59.82%), Query Frame = 1
Query: 106 HTCAHVMAMAVQKLFPDAKVTIGPWIENG--FYYDFDMEPLG--DKDLKKIKKEMERIIS 165
H+ AH++ A+++ + ++ IGP G FYYD LG D I+ +
Sbjct: 147 HSSAHILGQALEQEY-GCQLCIGPCTTRGEGFYYDGFYGELGLSDNHFPSIEAGAAKAAK 206
Query: 166 RNLPLVREEVSRDEAQRRIEAINEPYKMELLESIKED-PITIYHIGNEWWDLCAGPHVES 225
P R EV++D+A N +K+EL+ + D IT+Y G DLC GPH+ +
Sbjct: 207 EAQPFERIEVTKDQALEMFSENN--FKVELINGLPADMTITVYRCG-PLVDLCRGPHIPN 266
Query: 226 TGKINKRAVELETVAGAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRL 285
T + +A + + AYW+GD+ LQR+YG ++ ++QLK YL F EEAK+ DHR L
Sbjct: 267 TSFV--KAFKCLRASSAYWKGDKDRESLQRVYGISYPDQKQLKKYLQFLEEAKKYDHRLL 326
Query: 286 GKDLDLFSIQDDAGGGLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKT 345
G+ +LF G F+ P G V + + D K + RGY + TP++ ELW+T
Sbjct: 327 GQKQELF-FSHQLSPGSYFFLPLGTRVYNRLMDFIKNQYWHRGYTEVITPNMYNMELWQT 386
Query: 346 SGHSDFYGENMYNQMDVENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYE 405
SGH+D Y +NM+ ++E + + L+PMNCP H L ++ R+ SY++ P+R+A+ G ++R E
Sbjct: 387 SGHADNYKDNMFT-FNIEKQEFGLKPMNCPGHCLIFQHRVRSYRELPMRLADFGVLHRNE 446
Query: 406 LSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPP 465
SG+L GL RVR F QDDAHIFC +Q+K E++GVL+ + + FGF +E+ LSTRP
Sbjct: 447 ASGALSGLTRVRRFQQDDAHIFCTTEQVKGEVQGVLEFIDYVYKVFGFT-YELKLSTRPE 506
Query: 466 KSVGNDEIWEKATSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQV 525
K +G+ E W+KA + LK+A+ G ++EG GAFYGPKIDI + DA+ RK+QC+T+Q+
Sbjct: 507 KYLGDLETWDKAEADLKEAIEAFGKPLVLNEGDGAFYGPKIDITVSDAMNRKFQCATLQL 566
Query: 526 DFNLPQRFDITY-VDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRV 585
DF LP RF++ Y + ++K RP+MIHRAVLGS+ER F IL+E+Y G +P W+SP Q V
Sbjct: 567 DFQLPIRFNLEYAAEDEAKKSRPVMIHRAVLGSVERMFAILLEHYKGKWPFWISPRQAIV 626
Query: 586 LPVTDAQLSYCKEVSEKLRASGVRAEVCHGER-LPKLIRNAETQKIPLMAVVGAKEVEMQ 645
P+++ Y ++V ++++ +G + +R + K +R A+ + + VVG E
Sbjct: 627 CPISEKSQQYAEKVQKQIKDAGFYVDADLTDRKIDKKVREAQLAQYNYILVVGETEAATG 686
Query: 646 TVTVRSRFGGEIGTIAVDDFVGRIK 664
V+VR R +++D + K
Sbjct: 687 QVSVRVRDNAAHSVKSIEDLLEEFK 702
BLAST of Cucsa.100510 vs. TAIR10
Match:
AT1G17960.1 (AT1G17960.1 Threonyl-tRNA synthetase)
HSP 1 Score: 233.4 bits (594), Expect = 4.1e-61
Identity = 127/287 (44.25%), Postives = 179/287 (62.37%), Query Frame = 1
Query: 387 PIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAHIFCLDDQIKDEIRGVLDLTEDLLLQF 446
PIR+AE G ++R E SG+L G+ RVR F QDDAHIFC DQ+++E++GVLD + + F
Sbjct: 171 PIRMAEFGVLHRNEDSGALGGMTRVRRFVQDDAHIFCRVDQVEEEVKGVLDFIDYVYRIF 230
Query: 447 GFNEFEVNLSTRPPKSVGNDEIWEKATSALKDALGDKGWGYQIDEGGGAFYGPKIDIKIE 506
GF +E+ LSTRP +G+ E W KA + L+ AL D G + I EG GAFYGPKIDI +
Sbjct: 231 GFT-YELTLSTRPKDHIGDLETWAKAENDLEKALDDFGKPWVIKEGDGAFYGPKIDITVS 290
Query: 507 DALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAG 566
DA RK+QC+TIQ+DF LP F + Y+ +E + P+MIH AVLGS+ER F IL+E+Y G
Sbjct: 291 DARNRKFQCATIQLDFQLPACFKLKYLSEKNEMEAPVMIHSAVLGSIERMFAILLEHYKG 350
Query: 567 DFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRASGVRAEVCHGER-LPKLIRNAETQKIP 626
+P WLSP Q V +++ SY K+V +++ G ++ +R L K + +A
Sbjct: 351 IWPFWLSPRQAIVCSLSEDCSSYAKQVQKQINEVGYYVDIDESDRSLRKKVADARDAPYN 410
Query: 627 LMAVVGAKEVEMQTVTVRSRFGGE----IGTIAVDDFVGRIKFASEN 669
+ VVG KEV VTVR R E + ++++ + KF + N
Sbjct: 411 YILVVGQKEVATGQVTVRLREDPEGRKDLPEMSIESLLDEFKFKTVN 456
BLAST of Cucsa.100510 vs. TAIR10
Match:
AT1G18130.1 (AT1G18130.1 Class II aaRS and biotin synthetases superfamily protein)
HSP 1 Score: 80.5 bits (197), Expect = 4.4e-15
Identity = 47/125 (37.60%), Postives = 69/125 (55.20%), Query Frame = 1
Query: 520 VDFNLPQRFDITYVDSNSEK-KRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVR 579
VDF+L RF + Y N +R ++IHRAVL S+ER F IL+E Y G FP WLSP Q
Sbjct: 17 VDFHLRDRFKLEYSAENETMIERSVLIHRAVLSSVERLFAILLEQYKGKFPFWLSPRQAI 76
Query: 580 VLPVTDAQLSYCKEVSEKLRASGVRAEVCHGER-LPKLIRNAETQKIPLMAVVGAKEVEM 639
V V++ SY +V +++ +G +V +R + ++ A+ + VVG +E
Sbjct: 77 VCSVSEDYRSYADKVRDQIHEAGYYVDVDTTDRNVSDKVQEAQVAHYNYILVVGDEEART 136
Query: 640 QTVTV 643
VTV
Sbjct: 137 GQVTV 141
BLAST of Cucsa.100510 vs. NCBI nr
Match:
gi|449453584|ref|XP_004144536.1| (PREDICTED: probable threonine--tRNA ligase, cytoplasmic [Cucumis sativus])
HSP 1 Score: 1365.9 bits (3534), Expect = 0.0e+00
Identity = 672/672 (100.00%), Postives = 672/672 (100.00%), Query Frame = 1
Query: 1 MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS 60
MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS
Sbjct: 1 MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS 60
Query: 61 ISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
ISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61 ISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
Query: 181 EAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
EAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR
Sbjct: 181 EAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
Query: 301 HPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
HPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
Query: 421 IFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480
IFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480
Query: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK
Sbjct: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
Query: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS 600
RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS
Sbjct: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS 600
Query: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVG 660
GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVG
Sbjct: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVG 660
Query: 661 RIKFASENRTPF 673
RIKFASENRTPF
Sbjct: 661 RIKFASENRTPF 672
BLAST of Cucsa.100510 vs. NCBI nr
Match:
gi|659124339|ref|XP_008462105.1| (PREDICTED: probable threonine--tRNA ligase, cytoplasmic [Cucumis melo])
HSP 1 Score: 1337.0 bits (3459), Expect = 0.0e+00
Identity = 656/672 (97.62%), Postives = 663/672 (98.66%), Query Frame = 1
Query: 1 MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS 60
MFVLQRMATTTGLLLHSPL+TPISPNVSAPRFFLAVSTKFA NRDR F PR KSN FSTS
Sbjct: 1 MFVLQRMATTTGLLLHSPLLTPISPNVSAPRFFLAVSTKFAGNRDRSFFPRKKSNVFSTS 60
Query: 61 ISVATDSSAPSISTQNEDLVDAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
ISVATDSSAPS+STQNEDLVDAHKDQT KIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF
Sbjct: 61 ISVATDSSAPSVSTQNEDLVDAHKDQTGKIVLPTNESSEKLLRIRHTCAHVMAMAVQKLF 120
Query: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI
Sbjct: 121 PDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQRRI 180
Query: 181 EAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGAYWR 240
AINEPYKME+LESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRA+ELETVAGAYWR
Sbjct: 181 GAINEPYKMEILESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAIELETVAGAYWR 240
Query: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEA+RRDHRRLGKDLDLFSIQDDAGGGLVFW
Sbjct: 241 GDEKNPMLQRIYGTAWETEEQLKAYLHFKEEARRRDHRRLGKDLDLFSIQDDAGGGLVFW 300
Query: 301 HPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
HPKGAVVRSIIEDLWKKLHRERGYD+LYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE
Sbjct: 301 HPKGAVVRSIIEDLWKKLHRERGYDLLYTPHVAKAELWKTSGHSDFYGENMYNQMDVENE 360
Query: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH
Sbjct: 361 LYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQDDAH 420
Query: 421 IFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480
IFCLDDQIKDEIRGVLDLTE+LLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL
Sbjct: 421 IFCLDDQIKDEIRGVLDLTEELLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALKDAL 480
Query: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK
Sbjct: 481 GDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNSEKK 540
Query: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS 600
RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS
Sbjct: 541 RPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKLRAS 600
Query: 601 GVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDDFVG 660
GVRAEVCH ERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTI VDDFVG
Sbjct: 601 GVRAEVCHSERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTITVDDFVG 660
Query: 661 RIKFASENRTPF 673
RIKFASENRTPF
Sbjct: 661 RIKFASENRTPF 672
BLAST of Cucsa.100510 vs. NCBI nr
Match:
gi|1009128110|ref|XP_015881052.1| (PREDICTED: threonine--tRNA ligase, chloroplastic/mitochondrial 2 [Ziziphus jujuba])
HSP 1 Score: 1109.4 bits (2868), Expect = 0.0e+00
Identity = 540/677 (79.76%), Postives = 601/677 (88.77%), Query Frame = 1
Query: 1 MFVLQRMATTTGLLLHSP-LVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFST 60
MF+LQRMA + L S L TP+ S P F + ++F L N NGFST
Sbjct: 1 MFILQRMANSYSLFTSSAFLSTPLLKTSSLPSHFHSNLSRFVSTTPFNALFNNNRNGFST 60
Query: 61 SISVATDSSAP-SISTQNEDLVDAHKDQTE---KIVLPTNESSEKLLRIRHTCAHVMAMA 120
S++VATDSS+ ++STQNE L D+T+ K+VLPTN+SS+KLLRIRH+CAHVMAMA
Sbjct: 61 SLAVATDSSSQIAVSTQNEKLNQTQNDKTDDFRKVVLPTNDSSDKLLRIRHSCAHVMAMA 120
Query: 121 VQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDE 180
VQK+FPDAKVTIGPWIENGFYYDFDM PL D+DLKKIKKEM+RII RNLPLVREEV+RDE
Sbjct: 121 VQKIFPDAKVTIGPWIENGFYYDFDMGPLTDRDLKKIKKEMDRIIGRNLPLVREEVTRDE 180
Query: 181 AQRRIEAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVA 240
AQRRI A+NEPYK+E+L+SIKEDPITIYHIG+EWWDLCAGPHVESTGKIN+RAVELE+VA
Sbjct: 181 AQRRIMALNEPYKLEILDSIKEDPITIYHIGDEWWDLCAGPHVESTGKINRRAVELESVA 240
Query: 241 GAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGG 300
GAYWRGDEKN MLQRIYGTAWE EEQLKAYLHFKEEAKRRDHRRLG+DLDLFSIQ++AGG
Sbjct: 241 GAYWRGDEKNQMLQRIYGTAWENEEQLKAYLHFKEEAKRRDHRRLGQDLDLFSIQNEAGG 300
Query: 301 GLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQM 360
GLVFWHPKGA+VR +IEDLWKK+H ERGYDMLYTPHVAKA+LW+ SGH DFY ENMY+QM
Sbjct: 301 GLVFWHPKGAIVRHMIEDLWKKIHIERGYDMLYTPHVAKADLWQISGHLDFYKENMYDQM 360
Query: 361 DVENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFT 420
+E+ELYQLRPMNCPYHIL YKR+L SY DFPIRVAELGTVYRYELSGSL GLFRVRGFT
Sbjct: 361 SIEDELYQLRPMNCPYHILIYKRKLHSYHDFPIRVAELGTVYRYELSGSLHGLFRVRGFT 420
Query: 421 QDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSA 480
QDDAHIFCL DQIKDEIRGVLDLTE++LLQFGF+++EVNLSTRP K+VG D+IWEKATSA
Sbjct: 421 QDDAHIFCLSDQIKDEIRGVLDLTEEMLLQFGFSKYEVNLSTRPEKAVGGDDIWEKATSA 480
Query: 481 LKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDS 540
LK+AL DKGW YQIDEGGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQRFDITYVDS
Sbjct: 481 LKEALDDKGWSYQIDEGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQRFDITYVDS 540
Query: 541 NSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSE 600
NSEKKRPIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSPIQ R+LPVTD Q YC EV++
Sbjct: 541 NSEKKRPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPIQARILPVTDTQREYCSEVAK 600
Query: 601 KLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAV 660
KL+A+G+RAEVCHGERLPKLIRNAETQKIPLMAVVG KEVE TVTVRSRF G+IGT+ V
Sbjct: 601 KLKANGIRAEVCHGERLPKLIRNAETQKIPLMAVVGPKEVETGTVTVRSRFAGDIGTLTV 660
Query: 661 DDFVGRIKFASENRTPF 673
+DFVGR K A+ENRT F
Sbjct: 661 EDFVGRTKSATENRTSF 677
BLAST of Cucsa.100510 vs. NCBI nr
Match:
gi|703161498|ref|XP_010112806.1| (Threonine--tRNA ligase [Morus notabilis])
HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 542/691 (78.44%), Postives = 605/691 (87.55%), Query Frame = 1
Query: 1 MFVLQRMATTTGLLL-HSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFST 60
MF+L RMAT+ L S + P SP+ S R L ++ +F R F NGFST
Sbjct: 1 MFILHRMATSQSLFAPSSSFLKPSSPS-SFNRLGLPLTRQF-----RAFALHGHRNGFST 60
Query: 61 SIS-VATDSSAPSISTQNEDLV---DAHKDQTEKIVLPTNESSEKLLRIRHTCAHVMAMA 120
+ S VAT+SSA + S +N +L D + + E++VLPTN+SSEKLLRIRHTCAHVMAMA
Sbjct: 61 AYSAVATESSAET-SVENLELETNGDENSQKEERVVLPTNDSSEKLLRIRHTCAHVMAMA 120
Query: 121 VQKLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDE 180
VQKLFPDAKVTIGPWIENGFYYDFDMEPL D+DLKKIKKEM+RI+ +NLPL+REEVSRDE
Sbjct: 121 VQKLFPDAKVTIGPWIENGFYYDFDMEPLTDRDLKKIKKEMDRIVGKNLPLIREEVSRDE 180
Query: 181 AQRRIEAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVA 240
AQRRI ++NEPYK+E+LESIKE+PITIYHIGNEWWDLCAGPHVESTGKIN+RAVELE+VA
Sbjct: 181 AQRRIMSLNEPYKLEILESIKEEPITIYHIGNEWWDLCAGPHVESTGKINRRAVELESVA 240
Query: 241 GAYWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGG 300
GAYWRGDEK PMLQRIYGTAWE E+QLKAYLHFKEEAKRRDHRRLG+DLDLFSIQ+DAGG
Sbjct: 241 GAYWRGDEKKPMLQRIYGTAWENEQQLKAYLHFKEEAKRRDHRRLGQDLDLFSIQNDAGG 300
Query: 301 GLVFWHPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQM 360
GLVFWHPKGAVVR++IEDLW+K+H ERGYD+LYTPHVAKA+LW+ SGH DFY ENMY+QM
Sbjct: 301 GLVFWHPKGAVVRNVIEDLWRKIHIERGYDLLYTPHVAKADLWQISGHLDFYRENMYDQM 360
Query: 361 DVENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFT 420
++E+ELYQLRPMNCPYHIL YKR+L SYQDFPIRVAELGTVYRYELSGSL GLFRVRGFT
Sbjct: 361 NIEDELYQLRPMNCPYHILIYKRKLHSYQDFPIRVAELGTVYRYELSGSLHGLFRVRGFT 420
Query: 421 QDDAHIFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSA 480
QDDAHIFCL DQIKDEIRGVLDLTE++LLQFGF+++EVNLSTRP KSVG+D+IWEKATSA
Sbjct: 421 QDDAHIFCLSDQIKDEIRGVLDLTEEMLLQFGFSKYEVNLSTRPEKSVGDDDIWEKATSA 480
Query: 481 LKDALGDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQ--------------VD 540
LKDAL DKGW YQIDEGGGAFYGPKID+KIEDALGRKWQCSTIQ VD
Sbjct: 481 LKDALDDKGWSYQIDEGGGAFYGPKIDLKIEDALGRKWQCSTIQAIPRLIFSRFYGKRVD 540
Query: 541 FNLPQRFDITYVDSNSEKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLP 600
FNLPQRFDITYVDS SEKKRPIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSPIQ RVLP
Sbjct: 541 FNLPQRFDITYVDSTSEKKRPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPIQARVLP 600
Query: 601 VTDAQLSYCKEVSEKLRASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVT 660
VTD QL YC EV++KL+A+G+RAEVCHGERLPKLIRNAETQKIPLMAVVGAKE+E TVT
Sbjct: 601 VTDTQLEYCNEVAKKLKANGIRAEVCHGERLPKLIRNAETQKIPLMAVVGAKELETGTVT 660
Query: 661 VRSRFGGEIGTIAVDDFVGRIKFASENRTPF 673
VRSRFGG++GT+ VDDFVGR K A ENRT F
Sbjct: 661 VRSRFGGDVGTMTVDDFVGRTKSAIENRTSF 684
BLAST of Cucsa.100510 vs. NCBI nr
Match:
gi|470114485|ref|XP_004293445.1| (PREDICTED: probable threonine--tRNA ligase, cytoplasmic [Fragaria vesca subsp. vesca])
HSP 1 Score: 1077.0 bits (2784), Expect = 0.0e+00
Identity = 526/675 (77.93%), Postives = 592/675 (87.70%), Query Frame = 1
Query: 1 MFVLQRMATTTGLLLHSPLVTPISPNVSAPRFFLAVSTKFARNRDRPFLPRNKSNGFSTS 60
M +LQRMATT+ LL SPL+ P S R ++ F R F NG STS
Sbjct: 1 MLILQRMATTSSSLLSSPLLKPSSFLSPLKRCVSQPTSDF-----RAFA--FSRNGLSTS 60
Query: 61 ISVATDSSAPSISTQNEDLVDAHKDQTEK---IVLPTNESSEKLLRIRHTCAHVMAMAVQ 120
+ A ++ P +STQ++ L +A KD+++K +VLP+NESSEKLLRIRHTCAHVMAMAVQ
Sbjct: 61 -AAAVAATEPHVSTQHDKLKEAQKDKSDKLDRVVLPSNESSEKLLRIRHTCAHVMAMAVQ 120
Query: 121 KLFPDAKVTIGPWIENGFYYDFDMEPLGDKDLKKIKKEMERIISRNLPLVREEVSRDEAQ 180
KL+PDAKVTIGPWIENGFYYDFDMEPL DK+LK+IKKEM+RII RNLPLVREEVSR+EAQ
Sbjct: 121 KLYPDAKVTIGPWIENGFYYDFDMEPLTDKELKRIKKEMDRIICRNLPLVREEVSREEAQ 180
Query: 181 RRIEAINEPYKMELLESIKEDPITIYHIGNEWWDLCAGPHVESTGKINKRAVELETVAGA 240
RRI +NEPYK+E+L+SIK+DPITIYHIGNEWWDLCAGPHVE TGKIN++AVELE++AGA
Sbjct: 181 RRISTLNEPYKLEILDSIKDDPITIYHIGNEWWDLCAGPHVEYTGKINRKAVELESIAGA 240
Query: 241 YWRGDEKNPMLQRIYGTAWETEEQLKAYLHFKEEAKRRDHRRLGKDLDLFSIQDDAGGGL 300
YWRGDEK PMLQRIYGTAWE EEQL+AYLHFKEEAKRRDHRRLG+ LDLFSIQ+DAGGGL
Sbjct: 241 YWRGDEKKPMLQRIYGTAWENEEQLQAYLHFKEEAKRRDHRRLGQVLDLFSIQNDAGGGL 300
Query: 301 VFWHPKGAVVRSIIEDLWKKLHRERGYDMLYTPHVAKAELWKTSGHSDFYGENMYNQMDV 360
VFWHPKGA+VR +IEDLWKK H ERGYD+LYTPHVAKA+LW+ SGH DFY ENMYNQMDV
Sbjct: 301 VFWHPKGAIVRHVIEDLWKKTHIERGYDLLYTPHVAKADLWQISGHLDFYRENMYNQMDV 360
Query: 361 ENELYQLRPMNCPYHILFYKRRLTSYQDFPIRVAELGTVYRYELSGSLRGLFRVRGFTQD 420
E+ELYQLRPMNCPYHIL YKRR SY DFPIRVAELGTVYRYELSGSL GLFRVRGFTQD
Sbjct: 361 EDELYQLRPMNCPYHILIYKRRRHSYNDFPIRVAELGTVYRYELSGSLHGLFRVRGFTQD 420
Query: 421 DAHIFCLDDQIKDEIRGVLDLTEDLLLQFGFNEFEVNLSTRPPKSVGNDEIWEKATSALK 480
DAHIFCL+DQIKDEIRGVLDLTE+LLL+FGF+++EVNLSTRP KSVG+D+IWEKATSAL+
Sbjct: 421 DAHIFCLEDQIKDEIRGVLDLTEELLLKFGFSKYEVNLSTRPEKSVGDDDIWEKATSALR 480
Query: 481 DALGDKGWGYQIDEGGGAFYGPKIDIKIEDALGRKWQCSTIQVDFNLPQRFDITYVDSNS 540
DAL DKGW YQID+GGGAFYGPKID+KIEDALGRKWQCSTIQVDFNLPQRFDITY+DSNS
Sbjct: 481 DALEDKGWSYQIDDGGGAFYGPKIDLKIEDALGRKWQCSTIQVDFNLPQRFDITYIDSNS 540
Query: 541 EKKRPIMIHRAVLGSLERFFGILIENYAGDFPLWLSPIQVRVLPVTDAQLSYCKEVSEKL 600
EKKRPIMIHRAVLGSLERFFG+LIE+YAGDFPLWLSP+Q VLPVTD QL YCKE++ KL
Sbjct: 541 EKKRPIMIHRAVLGSLERFFGVLIEHYAGDFPLWLSPVQAHVLPVTDTQLDYCKELTNKL 600
Query: 601 RASGVRAEVCHGERLPKLIRNAETQKIPLMAVVGAKEVEMQTVTVRSRFGGEIGTIAVDD 660
+ SG+R E+CHGERLPKLIRN+E KIPLMAVVGAKEVE TVTVRSR G + GT+++DD
Sbjct: 601 KVSGIRGELCHGERLPKLIRNSEMLKIPLMAVVGAKEVESGTVTVRSRSGDDPGTMSIDD 660
Query: 661 FVGRIKFASENRTPF 673
FV RIK A E+RT F
Sbjct: 661 FVSRIKSAIESRTSF 667
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
SYTM2_ARATH | 5.1e-308 | 77.26 | Threonine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana GN... | [more] |
SYT_SYNPW | 1.8e-220 | 62.20 | Threonine--tRNA ligase OS=Synechococcus sp. (strain WH7803) GN=thrS PE=3 SV=1 | [more] |
SYT_SYNS3 | 3.1e-220 | 62.03 | Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9311) GN=thrS PE=3 SV=1 | [more] |
SYT_SYNS9 | 5.3e-220 | 61.99 | Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9902) GN=thrS PE=3 SV=1 | [more] |
SYT_SYNSC | 8.4e-218 | 61.00 | Threonine--tRNA ligase OS=Synechococcus sp. (strain CC9605) GN=thrS PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0K179_CUCSA | 0.0e+00 | 100.00 | Uncharacterized protein OS=Cucumis sativus GN=Csa_7G033430 PE=3 SV=1 | [more] |
W9SJL8_9ROSA | 0.0e+00 | 78.44 | Threonine--tRNA ligase OS=Morus notabilis GN=L484_020038 PE=3 SV=1 | [more] |
A0A067K3R0_JATCU | 3.6e-310 | 82.69 | Uncharacterized protein OS=Jatropha curcas GN=JCGZ_13804 PE=3 SV=1 | [more] |
M5WYT6_PRUPE | 1.1e-309 | 77.06 | Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002440mg PE=3 SV=1 | [more] |
B9IAN8_POPTR | 1.4e-307 | 75.73 | Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s16030g PE=3 SV=2 | [more] |