Cucsa.089980 (gene) Cucumber (Gy14) v1

NameCucsa.089980
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionGDP-L-fucose synthase
Locationscaffold00894 : 1390170 .. 1391543 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCCCTCACATATTCCTTCCTCCTCTCGCCGCCTGCCGACGATTCTCTCCTTCCACTTCCCACGATGGGCGGCGCCGCCGACAACGGTAATCTCTCTCTACATTCAGTGGCGGTTCGCGTTTCTTAGATCTCTGAATCCTTTCTGAAATTTCTCTTTTCTCTTTGATTTTTGCAGCTTCATCGGGATCCTCGTTTCTCTTCGATAAATCGGCGAAGATCTTTGTGGCTGGTCACCGTGGACTCGTCGGTTCCGCCATTGTCCGAAAACTCCAGCAGCTTGGATTCACGAATCTCATTCTTCGTTCTCACTCTGAGCTTGACCTAACTCGCCAATCCGATGTCGAATCGTTCTTCGCTAATGAAAAACCTCGATTCGTTATCCTCGCCGCCGCGAAAGTTGGTGGAATTCACGCTAACAACACCTATCCAGCGGATTTCATCGCCATTAACCTTCAAATCCAAACTAATGTCATCGATTCAGCCTACCGCTATGGCGTTGAGAAGCTACTATTTCTCGGTTCATCATGTATTTATCCTAAATTCGCTCCACAACCGATTCCTGAAGACGCATTACTAACCGGTCCTCTGGAGCCGACAAACGAGTGGTACGCGGTGGCGAAGATCGCCGGAATCAAAATGTGTCAAGCATATCGAATTCAATACAAATGGGATGCAATTTCAGGAATGCCGACTAATCTATACGGTCCAAACGATAATTTCCACCCGGAGAATTCACACGTATTGCCGGCATTGATGAGGAGATTTCATGAGGCAAAGGTTAAAGGAGCAAAAGAAGTGGTGGTTTGGGGAAGTGGAAGCCCTTTGAGGGAATTTCTTCACGTCGACGATTTGGCCGACGCCGTCGTTTTCCTCATGGAGGAATACAGTGATTTGGGGCATTTGAATGTTGGAAGTGGGAAGGAGGTGAGTATCAAGGAGCTGGCTGAATTGGTGAAAGAAGTGGTGGGATTTGAAGGAGATCTTGTTTGGGATAAATCAAAACCTGATGGAACTCCAAGGAAATTGATGGACAGTTCTAAGCTTGCTGAATTGGGATGGAATCCAAAGATTTCTCTCAAAGATGGGCTTGTGGATACTTACAAATGGTATGTTCAAAATGTGCAGCAATGAAGAAATGAACAGGAAAAAACACAGGTTAGTCCACCATTTTCAATCATCTGTTTGATCCTTTTGTTGTGATTGTAGTTTAAAAATCTATATTACTGAATCTAATTTGCAAGATATGAAGCTTTTATTGTTTTCTGATATTATATCATCTACGATTACATCCGCTATAGAGATTCTGTTTGATCTTTCCTTACTACTAGTAGATTGAACCAAATACAATTCCGAATAAATTTTGCATTTTGGAGA

mRNA sequence

CGCCCTCACATATTCCTTCCTCCTCTCGCCGCCTGCCGACGATTCTCTCCTTCCACTTCCCACGATGGGCGGCGCCGCCGACAACGCTTCATCGGGATCCTCGTTTCTCTTCGATAAATCGGCGAAGATCTTTGTGGCTGGTCACCGTGGACTCGTCGGTTCCGCCATTGTCCGAAAACTCCAGCAGCTTGGATTCACGAATCTCATTCTTCGTTCTCACTCTGAGCTTGACCTAACTCGCCAATCCGATGTCGAATCGTTCTTCGCTAATGAAAAACCTCGATTCGTTATCCTCGCCGCCGCGAAAGTTGGTGGAATTCACGCTAACAACACCTATCCAGCGGATTTCATCGCCATTAACCTTCAAATCCAAACTAATGTCATCGATTCAGCCTACCGCTATGGCGTTGAGAAGCTACTATTTCTCGGTTCATCATGTATTTATCCTAAATTCGCTCCACAACCGATTCCTGAAGACGCATTACTAACCGGTCCTCTGGAGCCGACAAACGAGTGGTACGCGGTGGCGAAGATCGCCGGAATCAAAATGTGTCAAGCATATCGAATTCAATACAAATGGGATGCAATTTCAGGAATGCCGACTAATCTATACGGTCCAAACGATAATTTCCACCCGGAGAATTCACACGTATTGCCGGCATTGATGAGGAGATTTCATGAGGCAAAGGTTAAAGGAGCAAAAGAAGTGGTGGTTTGGGGAAGTGGAAGCCCTTTGAGGGAATTTCTTCACGTCGACGATTTGGCCGACGCCGTCGTTTTCCTCATGGAGGAATACAGTGATTTGGGGCATTTGAATGTTGGAAGTGGGAAGGAGGTGAGTATCAAGGAGCTGGCTGAATTGGTGAAAGAAGTGGTGGGATTTGAAGGAGATCTTGTTTGGGATAAATCAAAACCTGATGGAACTCCAAGGAAATTGATGGACAGTTCTAAGCTTGCTGAATTGGGATGGAATCCAAAGATTTCTCTCAAAGATGGGCTTGTGGATACTTACAAATGGTATGTTCAAAATGTGCAGCAATGAAGAAATGAACAGGAAAAAACACAGGTTAGTCCACCATTTTCAATCATCTGTTTGATCCTTTTGTTGTGATTGTAGTTTAAAAATCTATATTACTGAATCTAATTTGCAAGATATGAAGCTTTTATTGTTTTCTGATATTATATCATCTACGATTACATCCGCTATAGAGATTCTGTTTGATCTTTCCTTACTACTAGTAGATTGAACCAAATACAATTCCGAATAAATTTTGCATTTTGGAGA

Coding sequence (CDS)

ATGGGCGGCGCCGCCGACAACGCTTCATCGGGATCCTCGTTTCTCTTCGATAAATCGGCGAAGATCTTTGTGGCTGGTCACCGTGGACTCGTCGGTTCCGCCATTGTCCGAAAACTCCAGCAGCTTGGATTCACGAATCTCATTCTTCGTTCTCACTCTGAGCTTGACCTAACTCGCCAATCCGATGTCGAATCGTTCTTCGCTAATGAAAAACCTCGATTCGTTATCCTCGCCGCCGCGAAAGTTGGTGGAATTCACGCTAACAACACCTATCCAGCGGATTTCATCGCCATTAACCTTCAAATCCAAACTAATGTCATCGATTCAGCCTACCGCTATGGCGTTGAGAAGCTACTATTTCTCGGTTCATCATGTATTTATCCTAAATTCGCTCCACAACCGATTCCTGAAGACGCATTACTAACCGGTCCTCTGGAGCCGACAAACGAGTGGTACGCGGTGGCGAAGATCGCCGGAATCAAAATGTGTCAAGCATATCGAATTCAATACAAATGGGATGCAATTTCAGGAATGCCGACTAATCTATACGGTCCAAACGATAATTTCCACCCGGAGAATTCACACGTATTGCCGGCATTGATGAGGAGATTTCATGAGGCAAAGGTTAAAGGAGCAAAAGAAGTGGTGGTTTGGGGAAGTGGAAGCCCTTTGAGGGAATTTCTTCACGTCGACGATTTGGCCGACGCCGTCGTTTTCCTCATGGAGGAATACAGTGATTTGGGGCATTTGAATGTTGGAAGTGGGAAGGAGGTGAGTATCAAGGAGCTGGCTGAATTGGTGAAAGAAGTGGTGGGATTTGAAGGAGATCTTGTTTGGGATAAATCAAAACCTGATGGAACTCCAAGGAAATTGATGGACAGTTCTAAGCTTGCTGAATTGGGATGGAATCCAAAGATTTCTCTCAAAGATGGGCTTGTGGATACTTACAAATGGTATGTTCAAAATGTGCAGCAATGA

Protein sequence

MGGAADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQSDVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLFLGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPTNLYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFLMEEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELGWNPKISLKDGLVDTYKWYVQNVQQ*
BLAST of Cucsa.089980 vs. Swiss-Prot
Match: FCL2_ARATH (Putative GDP-L-fucose synthase 2 OS=Arabidopsis thaliana GN=GER2 PE=1 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 2.9e-155
Identity = 269/324 (83.02%), Postives = 290/324 (89.51%), Query Frame = 1

Query: 5   ADNASS---GSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQS 64
           ADN  S     SF+ +KSAKIFVAGHRGLVGSAIVRKLQ  GFTNL+LR+HSELDLT QS
Sbjct: 2   ADNTGSEMKSGSFMLEKSAKIFVAGHRGLVGSAIVRKLQDQGFTNLVLRTHSELDLTSQS 61

Query: 65  DVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLFL 124
           DVESFFA EKP +VILAAAKVGGIHANNTYPADFI +NLQIQTNVI SAY +GV+KLLFL
Sbjct: 62  DVESFFATEKPVYVILAAAKVGGIHANNTYPADFIGVNLQIQTNVIHSAYTHGVKKLLFL 121

Query: 125 GSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPTN 184
           GSSCIYPKFAPQPIPE ALLTGPLEPTNEWYA+AKIAGIKMCQAYR+Q++WDAISGMPTN
Sbjct: 122 GSSCIYPKFAPQPIPESALLTGPLEPTNEWYAIAKIAGIKMCQAYRLQHQWDAISGMPTN 181

Query: 185 LYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFLM 244
           LYG NDNFHPENSHVLPALMRRFHEAK   A EVVVWGSGSPLREFLHVDDLADA VFLM
Sbjct: 182 LYGQNDNFHPENSHVLPALMRRFHEAKANNADEVVVWGSGSPLREFLHVDDLADACVFLM 241

Query: 245 EEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELG 304
           ++YS   H+NVGSG EV+IKELAELVKEVVGF+G LVWD +KPDGTPRKLMDSSKLA LG
Sbjct: 242 DQYSGFEHVNVGSGVEVTIKELAELVKEVVGFKGKLVWDTTKPDGTPRKLMDSSKLASLG 301

Query: 305 WNPKISLKDGLVDTYKWYVQNVQQ 326
           W PKISLKDGL  TY+WY++NV Q
Sbjct: 302 WTPKISLKDGLSQTYEWYLENVVQ 325

BLAST of Cucsa.089980 vs. Swiss-Prot
Match: FCL1_ARATH (GDP-L-fucose synthase 1 OS=Arabidopsis thaliana GN=GER1 PE=1 SV=3)

HSP 1 Score: 537.3 bits (1383), Expect = 1.1e-151
Identity = 259/319 (81.19%), Postives = 288/319 (90.28%), Query Frame = 1

Query: 5   ADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQSDVE 64
           A+   S  S + DKSAKIFVAGHRGLVGSAIVRKLQ+ GFTNL+L++H+ELDLTRQ+DVE
Sbjct: 2   AETIGSEVSSMSDKSAKIFVAGHRGLVGSAIVRKLQEQGFTNLVLKTHAELDLTRQADVE 61

Query: 65  SFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLFLGSS 124
           SFF+ EKP +VILAAAKVGGIHANNTYPADFI +NLQIQTNVI SAY +GV+KLLFLGSS
Sbjct: 62  SFFSQEKPVYVILAAAKVGGIHANNTYPADFIGVNLQIQTNVIHSAYEHGVKKLLFLGSS 121

Query: 125 CIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPTNLYG 184
           CIYPKFAPQPIPE ALLT  LEPTNEWYA+AKIAGIK CQAYRIQ+ WDAISGMPTNLYG
Sbjct: 122 CIYPKFAPQPIPESALLTASLEPTNEWYAIAKIAGIKTCQAYRIQHGWDAISGMPTNLYG 181

Query: 185 PNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFLMEEY 244
           PNDNFHPENSHVLPALMRRFHEAKV GA+EVVVWG+GSPLREFLHVDDLADA VFL++ Y
Sbjct: 182 PNDNFHPENSHVLPALMRRFHEAKVNGAEEVVVWGTGSPLREFLHVDDLADACVFLLDRY 241

Query: 245 SDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELGWNP 304
           S L H+N+GSG+EV+I+ELAELVKEVVGFEG L WD +KPDGTPRKLMDSSKLA LGW P
Sbjct: 242 SGLEHVNIGSGQEVTIRELAELVKEVVGFEGKLGWDCTKPDGTPRKLMDSSKLASLGWTP 301

Query: 305 KISLKDGLVDTYKWYVQNV 324
           K+SL+DGL  TY WY++NV
Sbjct: 302 KVSLRDGLSQTYDWYLKNV 320

BLAST of Cucsa.089980 vs. Swiss-Prot
Match: FCL1_ORYSJ (Probable GDP-L-fucose synthase 1 OS=Oryza sativa subsp. japonica GN=Os06g0652400 PE=2 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 1.3e-147
Identity = 252/322 (78.26%), Postives = 286/322 (88.82%), Query Frame = 1

Query: 3   GAADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQSD 62
           G    A   +SFL DK  K+FVAGHRGLVGSAI+R L  LGFTN+++R+H+ELDLTRQSD
Sbjct: 2   GTVTTADPHASFLADKGGKVFVAGHRGLVGSAILRHLVSLGFTNVVVRTHAELDLTRQSD 61

Query: 63  VESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYG-VEKLLFL 122
           VE+FFA E PR+V+LAAAKVGGIHAN+T+PADFIA NLQIQTNV+D+A + G V KLLFL
Sbjct: 62  VEAFFAAELPRYVVLAAAKVGGIHANSTFPADFIAANLQIQTNVVDAALKCGSVRKLLFL 121

Query: 123 GSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPTN 182
           GSSCIYPKFAPQPIPE++LL+GPLEPTNEWYAVAKIAGIKMCQAYRIQ+ +DAIS MPTN
Sbjct: 122 GSSCIYPKFAPQPIPENSLLSGPLEPTNEWYAVAKIAGIKMCQAYRIQHGFDAISAMPTN 181

Query: 183 LYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFLM 242
           LYGP DNFHPENSHVLPAL+RRFHEAK   A EVVVWG+GSPLREFLHVDDLADAV+FLM
Sbjct: 182 LYGPQDNFHPENSHVLPALIRRFHEAKASNAAEVVVWGTGSPLREFLHVDDLADAVIFLM 241

Query: 243 EEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELG 302
           + YS L H+NVGSG EV+IKELAELVKEVVGF+G LVWD SKPDGTPRKLMDSSK+ E+G
Sbjct: 242 DHYSGLEHVNVGSGSEVTIKELAELVKEVVGFQGKLVWDSSKPDGTPRKLMDSSKIQEMG 301

Query: 303 WNPKISLKDGLVDTYKWYVQNV 324
           W PK+ LK+GLV+TYKWYV+NV
Sbjct: 302 WKPKVPLKEGLVETYKWYVENV 323

BLAST of Cucsa.089980 vs. Swiss-Prot
Match: FCL2_ORYSJ (Putative GDP-L-fucose synthase 2 OS=Oryza sativa subsp. japonica GN=Os06g0652300 PE=3 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 2.9e-107
Identity = 187/318 (58.81%), Postives = 248/318 (77.99%), Query Frame = 1

Query: 6   DNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQSDVES 65
           D  ++  SFL DKSAK+F+AGHRG+VGSA+ RKL  LGFTN+++R+ +ELDL  Q+ VE+
Sbjct: 21  DGDAAAVSFLGDKSAKVFIAGHRGMVGSAVHRKLDALGFTNVVVRTRAELDLACQAAVEA 80

Query: 66  FFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYG-VEKLLFLGSS 125
           FFA E PR+VILAAAKVGG+HA++  PA+++  NL+I  NV+D+A R G V KLL L SS
Sbjct: 81  FFAAELPRYVILAAAKVGGVHASSAAPAEYLTENLRITVNVVDAARRCGSVRKLLVLASS 140

Query: 126 CIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPTNLYG 185
            IYP  APQP PE ALLTGP    +EWYA+ KIAGIKMCQA R +Y  DAI+  P NLYG
Sbjct: 141 TIYPADAPQPTPESALLTGPPAEGSEWYAIPKIAGIKMCQAVRAEYGLDAIAAAPNNLYG 200

Query: 186 PNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFLMEEY 245
           P   F PE+SHV+PAL+RRFH AK++GA EV VWGSG+  REF HVDDLA+AVV LME Y
Sbjct: 201 PRHPFPPEHSHVIPALIRRFHRAKLEGAGEVAVWGSGAAAREFTHVDDLAEAVVVLMERY 260

Query: 246 SDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELGWNP 305
           S   H+NVGSG+EV+++ELAE V+ VVG+EG + WD ++P+G  R+++DS ++ +LGW P
Sbjct: 261 SGEEHVNVGSGEEVTVRELAEAVRGVVGYEGVVAWDAARPEGVARRVVDSGRMRKLGWEP 320

Query: 306 KISLKDGLVDTYKWYVQN 323
           +++L+DG+ D Y++Y+++
Sbjct: 321 RVALRDGIQDLYRFYLRH 338

BLAST of Cucsa.089980 vs. Swiss-Prot
Match: FCL_SINFN (GDP-L-fucose synthase OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) GN=fcl PE=3 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 5.7e-103
Identity = 184/303 (60.73%), Postives = 235/303 (77.56%), Query Frame = 1

Query: 21  KIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQSDVESFFANEKPRFVILAAA 80
           +I+VAGH+G+VGSAI+R L       +I+    +LDLTRQ +VE F   EKP  VI+AAA
Sbjct: 10  RIWVAGHKGMVGSAIIRSLASED-CEVIVADRQKLDLTRQEEVEKFLLKEKPHAVIMAAA 69

Query: 81  KVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLFLGSSCIYPKFAPQPIPEDAL 140
           KVGGI AN+T PADFI  NL ++ NVI+ ++R GVEKLLFLGSSCIYPK+A QPI E+AL
Sbjct: 70  KVGGILANDTMPADFIYQNLIMEANVIEGSFRSGVEKLLFLGSSCIYPKYAAQPIREEAL 129

Query: 141 LTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPTNLYGPNDNFHPENSHVLPAL 200
           LTGPLEPTNEWYA+AKIAGIK+CQAYR QY  + IS MPTNLYGP D F   +SHV+PAL
Sbjct: 130 LTGPLEPTNEWYAIAKIAGIKLCQAYRKQYGANFISAMPTNLYGPRDKFDLNSSHVVPAL 189

Query: 201 MRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFLMEEYSDLGHLNVGSGKEVSI 260
           +R+ HEAK+K    + +WGSG+P R+FL+ +D +DA+VFL++ YS+  H+N+GSG E+SI
Sbjct: 190 IRKAHEAKIKDLGCLSIWGSGTPTRDFLYSEDCSDALVFLLKHYSETEHINIGSGGEISI 249

Query: 261 KELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELGWNPKISLKDGLVDTYKWYV 320
            ELA +V  VVGF+GD+V+D SKPDGTPRKL+ S +L  +GW PK SL+ GL  +Y+ +V
Sbjct: 250 IELAHIVCRVVGFKGDIVFDTSKPDGTPRKLLSSERLVSMGWRPKTSLELGLAKSYESFV 309

Query: 321 QNV 324
            NV
Sbjct: 310 SNV 311

BLAST of Cucsa.089980 vs. TrEMBL
Match: A0A0A0LH41_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G036660 PE=3 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 1.1e-185
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 1

Query: 1   MGGAADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQ 60
           MGGAADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQ
Sbjct: 1   MGGAADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQ 60

Query: 61  SDVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLF 120
           SDVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLF
Sbjct: 61  SDVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLF 120

Query: 121 LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPT 180
           LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPT
Sbjct: 121 LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPT 180

Query: 181 NLYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFL 240
           NLYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFL
Sbjct: 181 NLYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFL 240

Query: 241 MEEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAEL 300
           MEEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAEL
Sbjct: 241 MEEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAEL 300

Query: 301 GWNPKISLKDGLVDTYKWYVQNVQQ 326
           GWNPKISLKDGLVDTYKWYVQNVQQ
Sbjct: 301 GWNPKISLKDGLVDTYKWYVQNVQQ 325

BLAST of Cucsa.089980 vs. TrEMBL
Match: V7B2Y2_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_008G090200g PE=3 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 2.4e-164
Identity = 284/323 (87.93%), Postives = 305/323 (94.43%), Query Frame = 1

Query: 1   MGGAADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQ 60
           MG   +NA S +SFL  KSAK+FVAGHRGLVG+AIVRKL QLGFTNL+ RSH+ELDLTRQ
Sbjct: 1   MGSQDNNALSSNSFLAYKSAKVFVAGHRGLVGAAIVRKLTQLGFTNLVGRSHAELDLTRQ 60

Query: 61  SDVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLF 120
           SDVE+FFA+EKP FVI+AAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYR G +KLLF
Sbjct: 61  SDVEAFFASEKPEFVIVAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRNGAKKLLF 120

Query: 121 LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPT 180
           LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYA+AKIAGIKMCQAYRIQYKWDAISGMPT
Sbjct: 121 LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAIAKIAGIKMCQAYRIQYKWDAISGMPT 180

Query: 181 NLYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFL 240
           NLYGP DNFHPENSHVLPALMRRFHEAKVKGAKEVVVWG+GSPLREFLHVDDLADAVVF+
Sbjct: 181 NLYGPYDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGTGSPLREFLHVDDLADAVVFM 240

Query: 241 MEEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAEL 300
           ME+YS+L HLNVGSGKEV+IKELAEL+KEVVGFEGDLVWD +KPDGTPRKLMDSSKLA L
Sbjct: 241 MEKYSELDHLNVGSGKEVTIKELAELMKEVVGFEGDLVWDSTKPDGTPRKLMDSSKLASL 300

Query: 301 GWNPKISLKDGLVDTYKWYVQNV 324
           GW PK+SLKDGL DTYKWY++NV
Sbjct: 301 GWTPKVSLKDGLADTYKWYLENV 323

BLAST of Cucsa.089980 vs. TrEMBL
Match: M5XCN9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008630mg PE=3 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 3.1e-164
Identity = 287/325 (88.31%), Postives = 308/325 (94.77%), Query Frame = 1

Query: 1   MGGAADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQ 60
           M GA ++A+S S+ L DKSAKIFVAGHRGLVGSAIVRKLQ LGFTNL+LR H+ELDLTRQ
Sbjct: 1   MSGAINDATSPSA-LSDKSAKIFVAGHRGLVGSAIVRKLQALGFTNLVLRGHAELDLTRQ 60

Query: 61  SDVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLF 120
           +DVESFFA EKP+FVILAAAKVGGIHANNTYPADFIA+NLQIQTNVID++YR+GV+KLLF
Sbjct: 61  NDVESFFAVEKPQFVILAAAKVGGIHANNTYPADFIAVNLQIQTNVIDASYRFGVKKLLF 120

Query: 121 LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPT 180
           LGSSCIYPKFAPQPIPE ALLTGPLEPTNEWYA+AKIAGIKMCQAYRIQY WDAISGMPT
Sbjct: 121 LGSSCIYPKFAPQPIPESALLTGPLEPTNEWYAIAKIAGIKMCQAYRIQYNWDAISGMPT 180

Query: 181 NLYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFL 240
           NLYGP+DNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLAD VVFL
Sbjct: 181 NLYGPHDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADGVVFL 240

Query: 241 MEEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAEL 300
           ME YS L H+NVGSGKEV+IKELAELVKEVVGFEG+LVWD SKPDGTPRKLMDSSKLA L
Sbjct: 241 MENYSGLEHVNVGSGKEVTIKELAELVKEVVGFEGELVWDSSKPDGTPRKLMDSSKLAGL 300

Query: 301 GWNPKISLKDGLVDTYKWYVQNVQQ 326
           GW PKISLKDGLVDTYKWY++NV+Q
Sbjct: 301 GWTPKISLKDGLVDTYKWYLENVKQ 324

BLAST of Cucsa.089980 vs. TrEMBL
Match: A0A059CN64_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C01236 PE=3 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 5.3e-164
Identity = 286/325 (88.00%), Postives = 306/325 (94.15%), Query Frame = 1

Query: 1   MGGAADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQ 60
           MGG  D A  G SFL +KSAK+FVAGHRGLVGSAIV+KL+ LGFTNL+LR+H+ELDL RQ
Sbjct: 2   MGGPNDAA--GDSFLSEKSAKVFVAGHRGLVGSAIVQKLRDLGFTNLVLRTHAELDLARQ 61

Query: 61  SDVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLF 120
           SDVE FFA EKP FV+LAAAKVGGIHANNTYPADFIAINLQIQTNVIDSA+R+GV+KLLF
Sbjct: 62  SDVEDFFAAEKPPFVVLAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAHRHGVKKLLF 121

Query: 121 LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPT 180
           LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQY WDAISGMPT
Sbjct: 122 LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYGWDAISGMPT 181

Query: 181 NLYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFL 240
           NLYGPNDNFHPENSHVLPALMRRFHEAKV GAKEVVVWG+GSPLREFLHVDDLADAVVFL
Sbjct: 182 NLYGPNDNFHPENSHVLPALMRRFHEAKVSGAKEVVVWGTGSPLREFLHVDDLADAVVFL 241

Query: 241 MEEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAEL 300
           ME+YS LGH+NVGSGKEV+IKELAELVKEVVGFEG+LVWD SKPDGTPRKLMDSSKLA L
Sbjct: 242 MEKYSGLGHVNVGSGKEVTIKELAELVKEVVGFEGELVWDSSKPDGTPRKLMDSSKLASL 301

Query: 301 GWNPKISLKDGLVDTYKWYVQNVQQ 326
           GW PKISLK+GL DTYKWY++NV+Q
Sbjct: 302 GWTPKISLKEGLCDTYKWYLENVKQ 324

BLAST of Cucsa.089980 vs. TrEMBL
Match: A0A0B2R5G4_GLYSO (Putative GDP-L-fucose synthase 2 OS=Glycine soja GN=glysoja_005275 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 6.4e-162
Identity = 282/323 (87.31%), Postives = 303/323 (93.81%), Query Frame = 1

Query: 3   GAADN--ASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQ 62
           G+ DN  A S +SFL  KSAK+FVAGHRGLVGSAIVRKL QLGFTNL+L SH+ELDLTRQ
Sbjct: 2   GSQDNNVALSSNSFLAYKSAKVFVAGHRGLVGSAIVRKLTQLGFTNLVLHSHAELDLTRQ 61

Query: 63  SDVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLF 122
           SDVE+FFA+EKP FVI+AAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYR G +KLLF
Sbjct: 62  SDVEAFFASEKPEFVIVAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRNGAKKLLF 121

Query: 123 LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPT 182
           LGSSCIYPK+APQPIPEDALLTGPLEPTNEWYA+AKIAGIKMCQAYRIQ+KWDAISGMPT
Sbjct: 122 LGSSCIYPKYAPQPIPEDALLTGPLEPTNEWYAIAKIAGIKMCQAYRIQHKWDAISGMPT 181

Query: 183 NLYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFL 242
           NLYGP DNFHPENSHVLPALMRRFHEAKV GAKEVVVWG+GSPLREFLHVDDLADAVVF+
Sbjct: 182 NLYGPYDNFHPENSHVLPALMRRFHEAKVNGAKEVVVWGTGSPLREFLHVDDLADAVVFM 241

Query: 243 MEEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAEL 302
           ME+YS L HLNVGSGKEV+IKELAEL+KEVVGFEGDLVWD +KPDGTPRKLMDSSKLA L
Sbjct: 242 MEKYSGLEHLNVGSGKEVTIKELAELMKEVVGFEGDLVWDSTKPDGTPRKLMDSSKLASL 301

Query: 303 GWNPKISLKDGLVDTYKWYVQNV 324
           GW PK+SLKDGL DTYKWY++NV
Sbjct: 302 GWTPKVSLKDGLADTYKWYLENV 324

BLAST of Cucsa.089980 vs. TAIR10
Match: AT1G17890.1 (AT1G17890.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 549.3 bits (1414), Expect = 1.6e-156
Identity = 269/324 (83.02%), Postives = 290/324 (89.51%), Query Frame = 1

Query: 5   ADNASS---GSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQS 64
           ADN  S     SF+ +KSAKIFVAGHRGLVGSAIVRKLQ  GFTNL+LR+HSELDLT QS
Sbjct: 2   ADNTGSEMKSGSFMLEKSAKIFVAGHRGLVGSAIVRKLQDQGFTNLVLRTHSELDLTSQS 61

Query: 65  DVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLFL 124
           DVESFFA EKP +VILAAAKVGGIHANNTYPADFI +NLQIQTNVI SAY +GV+KLLFL
Sbjct: 62  DVESFFATEKPVYVILAAAKVGGIHANNTYPADFIGVNLQIQTNVIHSAYTHGVKKLLFL 121

Query: 125 GSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPTN 184
           GSSCIYPKFAPQPIPE ALLTGPLEPTNEWYA+AKIAGIKMCQAYR+Q++WDAISGMPTN
Sbjct: 122 GSSCIYPKFAPQPIPESALLTGPLEPTNEWYAIAKIAGIKMCQAYRLQHQWDAISGMPTN 181

Query: 185 LYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFLM 244
           LYG NDNFHPENSHVLPALMRRFHEAK   A EVVVWGSGSPLREFLHVDDLADA VFLM
Sbjct: 182 LYGQNDNFHPENSHVLPALMRRFHEAKANNADEVVVWGSGSPLREFLHVDDLADACVFLM 241

Query: 245 EEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELG 304
           ++YS   H+NVGSG EV+IKELAELVKEVVGF+G LVWD +KPDGTPRKLMDSSKLA LG
Sbjct: 242 DQYSGFEHVNVGSGVEVTIKELAELVKEVVGFKGKLVWDTTKPDGTPRKLMDSSKLASLG 301

Query: 305 WNPKISLKDGLVDTYKWYVQNVQQ 326
           W PKISLKDGL  TY+WY++NV Q
Sbjct: 302 WTPKISLKDGLSQTYEWYLENVVQ 325

BLAST of Cucsa.089980 vs. TAIR10
Match: AT1G73250.1 (AT1G73250.1 GDP-4-keto-6-deoxymannose-3,5-epimerase-4-reductase 1)

HSP 1 Score: 537.3 bits (1383), Expect = 6.4e-153
Identity = 259/319 (81.19%), Postives = 288/319 (90.28%), Query Frame = 1

Query: 5   ADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQSDVE 64
           A+   S  S + DKSAKIFVAGHRGLVGSAIVRKLQ+ GFTNL+L++H+ELDLTRQ+DVE
Sbjct: 2   AETIGSEVSSMSDKSAKIFVAGHRGLVGSAIVRKLQEQGFTNLVLKTHAELDLTRQADVE 61

Query: 65  SFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLFLGSS 124
           SFF+ EKP +VILAAAKVGGIHANNTYPADFI +NLQIQTNVI SAY +GV+KLLFLGSS
Sbjct: 62  SFFSQEKPVYVILAAAKVGGIHANNTYPADFIGVNLQIQTNVIHSAYEHGVKKLLFLGSS 121

Query: 125 CIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPTNLYG 184
           CIYPKFAPQPIPE ALLT  LEPTNEWYA+AKIAGIK CQAYRIQ+ WDAISGMPTNLYG
Sbjct: 122 CIYPKFAPQPIPESALLTASLEPTNEWYAIAKIAGIKTCQAYRIQHGWDAISGMPTNLYG 181

Query: 185 PNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFLMEEY 244
           PNDNFHPENSHVLPALMRRFHEAKV GA+EVVVWG+GSPLREFLHVDDLADA VFL++ Y
Sbjct: 182 PNDNFHPENSHVLPALMRRFHEAKVNGAEEVVVWGTGSPLREFLHVDDLADACVFLLDRY 241

Query: 245 SDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELGWNP 304
           S L H+N+GSG+EV+I+ELAELVKEVVGFEG L WD +KPDGTPRKLMDSSKLA LGW P
Sbjct: 242 SGLEHVNIGSGQEVTIRELAELVKEVVGFEGKLGWDCTKPDGTPRKLMDSSKLASLGWTP 301

Query: 305 KISLKDGLVDTYKWYVQNV 324
           K+SL+DGL  TY WY++NV
Sbjct: 302 KVSLRDGLSQTYDWYLKNV 320

BLAST of Cucsa.089980 vs. TAIR10
Match: AT5G28840.1 (AT5G28840.1 GDP-D-mannose 3',5'-epimerase)

HSP 1 Score: 90.5 bits (223), Expect = 2.1e-18
Identity = 81/327 (24.77%), Postives = 142/327 (43.43%), Query Frame = 1

Query: 18  KSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQSDVESFF---------- 77
           ++ KI + G  G + S I R+L+  G   +         +T     + F           
Sbjct: 26  ENLKISITGAGGFIASHIARRLKHEGHYVIASDWKKNEHMTEDMFCDEFHLVDLRVMENC 85

Query: 78  --ANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLFLGSSC 137
               E    V   AA +GG+    +  +  +  N  I  N+I++A   G+++  +  S+C
Sbjct: 86  LKVTEGVDHVFNLAADMGGMGFIQSNHSVIMYNNTMISFNMIEAARINGIKRFFYASSAC 145

Query: 138 IYPKFAPQPIPEDALLTG---PLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPTNL 197
           IYP+F        +L      P EP +  Y + K+A  ++C+ Y   +  +   G   N+
Sbjct: 146 IYPEFKQLETTNVSLKESDAWPAEPQDA-YGLEKLATEELCKHYNKDFGIECRIGRFHNI 205

Query: 198 YGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFLME 257
           YGP   +         A  R+   +         +WG G   R F  +D+  + V+ L +
Sbjct: 206 YGPFGTWKGGREKAPAAFCRKAQTS----TDRFEMWGDGLQTRSFTFIDECVEGVLRLTK 265

Query: 258 EYSDLGH-LNVGSGKEVSIKELAELVKEVVGFEGDL--VWDKSKPDGTPRKLMDSSKLAE 317
             SD    +N+GS + VS+ E+AE+V   + FE     +     P+G   +  D++ + E
Sbjct: 266 --SDFREPVNIGSDEMVSMNEMAEMV---LSFEEKKLPIHHIPGPEGVRGRNSDNNLIKE 325

Query: 318 -LGWNPKISLKDGLVDTYKWYVQNVQQ 326
            LGW P + LK+GL  TY W  + +++
Sbjct: 326 KLGWAPNMRLKEGLRITYFWIKEQIEK 342

BLAST of Cucsa.089980 vs. TAIR10
Match: AT1G78570.1 (AT1G78570.1 rhamnose biosynthesis 1)

HSP 1 Score: 76.6 bits (187), Expect = 3.1e-14
Identity = 55/193 (28.50%), Postives = 100/193 (51.81%), Query Frame = 1

Query: 137 EDALL----TGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPTNLYGPNDNFHPE 196
           EDAL+       L PTN  Y+  K     +  AY   Y    I+    N+YGPN    PE
Sbjct: 141 EDALVGNHEASQLLPTNP-YSATKAGAEMLVMAYGRSYGLPVITTRGNNVYGPNQ--FPE 200

Query: 197 NSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFLMEEYSDLGHL-N 256
                  L+ +F    ++G + + + G GS +R +L+ +D+A+A   ++ +  ++GH+ N
Sbjct: 201 K------LIPKFILLAMRG-QVLPIHGDGSNVRSYLYCEDVAEAFEVVLHK-GEVGHVYN 260

Query: 257 VGSGKEVSIKELAELVKEVVGF--EGDLVWDKSKPDGTPRKLMDSSKLAELGWNPKISLK 316
           +G+ KE  + ++A+ + ++     E ++ +  ++P    R  +D  KL +LGW+ + + +
Sbjct: 261 IGTKKERRVNDVAKDICKLFNMDPEANIKFVDNRPFNDQRYFLDDQKLKKLGWSERTTWE 320

Query: 317 DGLVDTYKWYVQN 323
           +GL  T  WY QN
Sbjct: 321 EGLKKTMDWYTQN 322

BLAST of Cucsa.089980 vs. TAIR10
Match: AT1G53500.1 (AT1G53500.1 NAD-dependent epimerase/dehydratase family protein)

HSP 1 Score: 76.3 bits (186), Expect = 4.0e-14
Identity = 53/181 (29.28%), Postives = 93/181 (51.38%), Query Frame = 1

Query: 145 LEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPTNLYGPNDNFHPENSHVLPALMRRF 204
           L PTN  Y+  K     +  AY   Y    I+    N+YGPN    PE       ++ +F
Sbjct: 155 LLPTNP-YSATKAGAEMLVMAYGRSYGLPVITTRGNNVYGPNQ--FPEK------MIPKF 214

Query: 205 HEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFLMEEYSDLGHL-NVGSGKEVSIKEL 264
               + G K + + G GS +R +L+ +D+A+A   ++ +  ++GH+ NVG+ +E  + ++
Sbjct: 215 ILLAMSG-KPLPIHGDGSNVRSYLYCEDVAEAFEVVLHK-GEIGHVYNVGTKRERRVIDV 274

Query: 265 AELVKEVVGF--EGDLVWDKSKPDGTPRKLMDSSKLAELGWNPKISLKDGLVDTYKWYVQ 323
           A  + ++ G   E  + + +++P    R  +D  KL +LGW  + + +DGL  T  WY Q
Sbjct: 275 ARDICKLFGKDPESSIQFVENRPFNDQRYFLDDQKLKKLGWQERTNWEDGLKKTMDWYTQ 324

BLAST of Cucsa.089980 vs. NCBI nr
Match: gi|449453946|ref|XP_004144717.1| (PREDICTED: putative GDP-L-fucose synthase 2 [Cucumis sativus])

HSP 1 Score: 657.1 bits (1694), Expect = 1.6e-185
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 1

Query: 1   MGGAADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQ 60
           MGGAADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQ
Sbjct: 1   MGGAADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQ 60

Query: 61  SDVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLF 120
           SDVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLF
Sbjct: 61  SDVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLF 120

Query: 121 LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPT 180
           LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPT
Sbjct: 121 LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPT 180

Query: 181 NLYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFL 240
           NLYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFL
Sbjct: 181 NLYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFL 240

Query: 241 MEEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAEL 300
           MEEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAEL
Sbjct: 241 MEEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAEL 300

Query: 301 GWNPKISLKDGLVDTYKWYVQNVQQ 326
           GWNPKISLKDGLVDTYKWYVQNVQQ
Sbjct: 301 GWNPKISLKDGLVDTYKWYVQNVQQ 325

BLAST of Cucsa.089980 vs. NCBI nr
Match: gi|659069931|ref|XP_008452484.1| (PREDICTED: putative GDP-L-fucose synthase 2 [Cucumis melo])

HSP 1 Score: 656.4 bits (1692), Expect = 2.7e-185
Identity = 324/325 (99.69%), Postives = 325/325 (100.00%), Query Frame = 1

Query: 1   MGGAADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQ 60
           MGGAADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNL+LRSHSELDLTRQ
Sbjct: 1   MGGAADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLLLRSHSELDLTRQ 60

Query: 61  SDVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLF 120
           SDVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLF
Sbjct: 61  SDVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLF 120

Query: 121 LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPT 180
           LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPT
Sbjct: 121 LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPT 180

Query: 181 NLYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFL 240
           NLYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFL
Sbjct: 181 NLYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFL 240

Query: 241 MEEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAEL 300
           MEEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAEL
Sbjct: 241 MEEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAEL 300

Query: 301 GWNPKISLKDGLVDTYKWYVQNVQQ 326
           GWNPKISLKDGLVDTYKWYVQNVQQ
Sbjct: 301 GWNPKISLKDGLVDTYKWYVQNVQQ 325

BLAST of Cucsa.089980 vs. NCBI nr
Match: gi|593359871|ref|XP_007140175.1| (hypothetical protein PHAVU_008G090200g [Phaseolus vulgaris])

HSP 1 Score: 586.3 bits (1510), Expect = 3.4e-164
Identity = 284/323 (87.93%), Postives = 305/323 (94.43%), Query Frame = 1

Query: 1   MGGAADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQ 60
           MG   +NA S +SFL  KSAK+FVAGHRGLVG+AIVRKL QLGFTNL+ RSH+ELDLTRQ
Sbjct: 1   MGSQDNNALSSNSFLAYKSAKVFVAGHRGLVGAAIVRKLTQLGFTNLVGRSHAELDLTRQ 60

Query: 61  SDVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLF 120
           SDVE+FFA+EKP FVI+AAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYR G +KLLF
Sbjct: 61  SDVEAFFASEKPEFVIVAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRNGAKKLLF 120

Query: 121 LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPT 180
           LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYA+AKIAGIKMCQAYRIQYKWDAISGMPT
Sbjct: 121 LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAIAKIAGIKMCQAYRIQYKWDAISGMPT 180

Query: 181 NLYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFL 240
           NLYGP DNFHPENSHVLPALMRRFHEAKVKGAKEVVVWG+GSPLREFLHVDDLADAVVF+
Sbjct: 181 NLYGPYDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGTGSPLREFLHVDDLADAVVFM 240

Query: 241 MEEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAEL 300
           ME+YS+L HLNVGSGKEV+IKELAEL+KEVVGFEGDLVWD +KPDGTPRKLMDSSKLA L
Sbjct: 241 MEKYSELDHLNVGSGKEVTIKELAELMKEVVGFEGDLVWDSTKPDGTPRKLMDSSKLASL 300

Query: 301 GWNPKISLKDGLVDTYKWYVQNV 324
           GW PK+SLKDGL DTYKWY++NV
Sbjct: 301 GWTPKVSLKDGLADTYKWYLENV 323

BLAST of Cucsa.089980 vs. NCBI nr
Match: gi|596147454|ref|XP_007222683.1| (hypothetical protein PRUPE_ppa008630mg [Prunus persica])

HSP 1 Score: 585.9 bits (1509), Expect = 4.4e-164
Identity = 287/325 (88.31%), Postives = 308/325 (94.77%), Query Frame = 1

Query: 1   MGGAADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQ 60
           M GA ++A+S S+ L DKSAKIFVAGHRGLVGSAIVRKLQ LGFTNL+LR H+ELDLTRQ
Sbjct: 1   MSGAINDATSPSA-LSDKSAKIFVAGHRGLVGSAIVRKLQALGFTNLVLRGHAELDLTRQ 60

Query: 61  SDVESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLF 120
           +DVESFFA EKP+FVILAAAKVGGIHANNTYPADFIA+NLQIQTNVID++YR+GV+KLLF
Sbjct: 61  NDVESFFAVEKPQFVILAAAKVGGIHANNTYPADFIAVNLQIQTNVIDASYRFGVKKLLF 120

Query: 121 LGSSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPT 180
           LGSSCIYPKFAPQPIPE ALLTGPLEPTNEWYA+AKIAGIKMCQAYRIQY WDAISGMPT
Sbjct: 121 LGSSCIYPKFAPQPIPESALLTGPLEPTNEWYAIAKIAGIKMCQAYRIQYNWDAISGMPT 180

Query: 181 NLYGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFL 240
           NLYGP+DNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLAD VVFL
Sbjct: 181 NLYGPHDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADGVVFL 240

Query: 241 MEEYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAEL 300
           ME YS L H+NVGSGKEV+IKELAELVKEVVGFEG+LVWD SKPDGTPRKLMDSSKLA L
Sbjct: 241 MENYSGLEHVNVGSGKEVTIKELAELVKEVVGFEGELVWDSSKPDGTPRKLMDSSKLAGL 300

Query: 301 GWNPKISLKDGLVDTYKWYVQNVQQ 326
           GW PKISLKDGLVDTYKWY++NV+Q
Sbjct: 301 GWTPKISLKDGLVDTYKWYLENVKQ 324

BLAST of Cucsa.089980 vs. NCBI nr
Match: gi|1009109657|ref|XP_015891344.1| (PREDICTED: putative GDP-L-fucose synthase 2 [Ziziphus jujuba])

HSP 1 Score: 585.5 bits (1508), Expect = 5.8e-164
Identity = 283/323 (87.62%), Postives = 306/323 (94.74%), Query Frame = 1

Query: 3   GAADNASSGSSFLFDKSAKIFVAGHRGLVGSAIVRKLQQLGFTNLILRSHSELDLTRQSD 62
           G+A N ++  SF+ DKSAKIFVAGHRGLVGSAIVRKLQ LG++NL+LR+H+ LDLTRQ+D
Sbjct: 2   GSATNETTSDSFISDKSAKIFVAGHRGLVGSAIVRKLQALGYSNLVLRTHAALDLTRQTD 61

Query: 63  VESFFANEKPRFVILAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGVEKLLFLG 122
           VESFFA EKP+FV+LAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYG +KLLFLG
Sbjct: 62  VESFFATEKPQFVVLAAAKVGGIHANNTYPADFIAINLQIQTNVIDSAYRYGAKKLLFLG 121

Query: 123 SSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYKWDAISGMPTNL 182
           SSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQY WDAISGMPTNL
Sbjct: 122 SSCIYPKFAPQPIPEDALLTGPLEPTNEWYAVAKIAGIKMCQAYRIQYGWDAISGMPTNL 181

Query: 183 YGPNDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGSGSPLREFLHVDDLADAVVFLME 242
           YGP DNFHPENSHVLPALMRRFHEAKVKGAKEVVVWG+GSPLREFLHVDDLADAVVFLM 
Sbjct: 182 YGPYDNFHPENSHVLPALMRRFHEAKVKGAKEVVVWGTGSPLREFLHVDDLADAVVFLMN 241

Query: 243 EYSDLGHLNVGSGKEVSIKELAELVKEVVGFEGDLVWDKSKPDGTPRKLMDSSKLAELGW 302
           +YS L H+NVGSGKEV+IKELAELVKEVVGFEG+LVWD +KPDGTPRKLMDS+KLA LGW
Sbjct: 242 KYSGLEHVNVGSGKEVTIKELAELVKEVVGFEGELVWDSTKPDGTPRKLMDSAKLAGLGW 301

Query: 303 NPKISLKDGLVDTYKWYVQNVQQ 326
            PKISLKDGLVDTYKWY++NV+Q
Sbjct: 302 TPKISLKDGLVDTYKWYLENVKQ 324

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
FCL2_ARATH2.9e-15583.02Putative GDP-L-fucose synthase 2 OS=Arabidopsis thaliana GN=GER2 PE=1 SV=1[more]
FCL1_ARATH1.1e-15181.19GDP-L-fucose synthase 1 OS=Arabidopsis thaliana GN=GER1 PE=1 SV=3[more]
FCL1_ORYSJ1.3e-14778.26Probable GDP-L-fucose synthase 1 OS=Oryza sativa subsp. japonica GN=Os06g0652400... [more]
FCL2_ORYSJ2.9e-10758.81Putative GDP-L-fucose synthase 2 OS=Oryza sativa subsp. japonica GN=Os06g0652300... [more]
FCL_SINFN5.7e-10360.73GDP-L-fucose synthase OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) GN=f... [more]
Match NameE-valueIdentityDescription
A0A0A0LH41_CUCSA1.1e-185100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_2G036660 PE=3 SV=1[more]
V7B2Y2_PHAVU2.4e-16487.93Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_008G090200g PE=3 SV=1[more]
M5XCN9_PRUPE3.1e-16488.31Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008630mg PE=3 SV=1[more]
A0A059CN64_EUCGR5.3e-16488.00Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C01236 PE=3 SV=1[more]
A0A0B2R5G4_GLYSO6.4e-16287.31Putative GDP-L-fucose synthase 2 OS=Glycine soja GN=glysoja_005275 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G17890.11.6e-15683.02 NAD(P)-binding Rossmann-fold superfamily protein[more]
AT1G73250.16.4e-15381.19 GDP-4-keto-6-deoxymannose-3,5-epimerase-4-reductase 1[more]
AT5G28840.12.1e-1824.77 GDP-D-mannose 3',5'-epimerase[more]
AT1G78570.13.1e-1428.50 rhamnose biosynthesis 1[more]
AT1G53500.14.0e-1429.28 NAD-dependent epimerase/dehydratase family protein[more]
Match NameE-valueIdentityDescription
gi|449453946|ref|XP_004144717.1|1.6e-185100.00PREDICTED: putative GDP-L-fucose synthase 2 [Cucumis sativus][more]
gi|659069931|ref|XP_008452484.1|2.7e-18599.69PREDICTED: putative GDP-L-fucose synthase 2 [Cucumis melo][more]
gi|593359871|ref|XP_007140175.1|3.4e-16487.93hypothetical protein PHAVU_008G090200g [Phaseolus vulgaris][more]
gi|596147454|ref|XP_007222683.1|4.4e-16488.31hypothetical protein PRUPE_ppa008630mg [Prunus persica][more]
gi|1009109657|ref|XP_015891344.1|5.8e-16487.62PREDICTED: putative GDP-L-fucose synthase 2 [Ziziphus jujuba][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001509Epimerase_deHydtase
IPR016040NAD(P)-bd_dom
Vocabulary: Biological Process
TermDefinition
GO:0009226nucleotide-sugar biosynthetic process
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
GO:0003824catalytic activity
GO:0050662coenzyme binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042351 'de novo' GDP-L-fucose biosynthetic process
biological_process GO:0006000 fructose metabolic process
biological_process GO:0006013 mannose metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0009226 nucleotide-sugar biosynthetic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0050662 coenzyme binding
molecular_function GO:0050577 GDP-L-fucose synthase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016491 oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.089980.1Cucsa.089980.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001509NAD-dependent epimerase/dehydratase, N-terminal domainPFAMPF01370Epimerasecoord: 22..253
score: 1.3
IPR016040NAD(P)-binding domainGENE3DG3DSA:3.40.50.720coord: 21..224
score: 3.5
IPR016040NAD(P)-binding domainunknownSSF51735NAD(P)-binding Rossmann-fold domainscoord: 18..323
score: 2.2
NoneNo IPR availableGENE3DG3DSA:3.90.25.10coord: 225..247
score: 4.9E-13coord: 300..323
score: 4.9
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 21..325
score: 6.0E
NoneNo IPR availablePANTHERPTHR10366:SF246GDP-L-FUCOSE SYNTHASE 1-RELATEDcoord: 21..325
score: 6.0E

The following gene(s) are paralogous to this gene:

None