Cucsa.019390 (gene) Cucumber (Gy14) v1

NameCucsa.019390
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionSubtilisin-like serine protease
Locationscaffold00276 : 286106 .. 292513 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCTTCTCGTCTTCTTGTCTTTGCTCTTTTCATCGTCGTAGGATGCGTTGCAGGGTTGGACGAGGACGAGGAAAAGGTTTTTGAATTAATAAATTCATTCGTCATTTTAATTGCTTTGTTATGGGTTTTTTTATTGTTTCTCTTATTGTCTTTGTGTTTTTTGCAGAATCATTATATTGTATTCTTGGAAAATAAACCAGTTTTAAATGAGGTTGATGTAGTAGAAACACATCTAAATCTTCTCATGTCAGTGAAGAAAAGGTGGGTAAATTCAAATGGTAAATGATTCAGTTTGATTTACATATTTAGTTTGTAAGCCTAAATATGTAGATATATCACGACTTTTTCTTTGTATTACAACATATTACTGATTCAACTAAAAGAAACTCTAGTATCACAGTAAACATATTTCCTTTATTATCAATTTGATGGTCATATATTTAAGTGATATAATATTTTATTTCAAGGGTTTTATACTGTTTTTATATATTTAATGTATTGTTATAGTAAGCCATGAATAGATACAGTAATTCAGAATTTTGAATACATATACACTACTTTTCATACAAAAATGTTAAATAGATTAATGTTTCACCTTACTAATATGTTTTTTTAATCACTCAAAACTATAATATTTAAAGAAAAACCTATTTATACTTTAACTGAAACATCCAATTAATTATCCTTTAATTTAAACTTTCAACTTAGTTAGGTAAGCAAATCAATTTAGGTTGATTATAGTTAATAAATGGAATTTTTTTCTTGCTTTCTAAATTTGATGGTAGAGAAAGGGTCCAAAGGGTGAAAGAAATCAACCCAAATCTGTGATTTTGTTCCTGAATTGTTAAATTGCACAATATCTCTATAAAGTGGAGCAAGAGGGAAGTCATATTAATGTTATAATGATGTCCAATAGGACTATATCCCATTTAAAAATATATATATATACCACTAAGTTAAACATGGTGTCATTTTACTATTACTATCACCTTGACCAAGTTAATTAATGAGATATGCTGCCTAACATTATAATGCTACCAAAGCATAATTAACCTACCATAAAATATTCGGTATCAACCCTTACTTAGAGATAAAAGTAATATAATTTAAGTCAAAATATTATTATTGTATGCTGTATAGTTTGAAGTTTGTGCTTACTTAATCTATATAAACCTTCAATTGTCCAACTTTAATTTCTAATTTTTAATAAATCTTTAAATTCAGTTTCTAAAAAATCATCTTTTAACAAAAAAAAACAAGTTAAATAAAAATATAGATAAATTTCAAATATAATGAAATAAACCCAAGCTTCTTAGAAAAATATCACTTATCTATAATGAACAAATATCACCACTTATCTATAATGAACGAGGATAGACTAAGATAAACTACTAGTTATATTTATGTGTATCATAACAATCACAAACAATAATATTAGTAAATATTTTCAAAAAATTTATCATTTTAAACGTGTAAATTAAAGATAATTTTGATTTAAGGAAAAGCATATTCACAATATGTTCTCAAAATTACAGTAAATAAAAAGGAAATTAAATGAATTTTTGTGGTTTTGTAGCCATGCCGAAGCAAGTGAATCGATGGTTTATAGTTACACGAAAAGCTTCAATGCCTTTGCTGCTAAACTTAGTGATGATGAAGCCAAATTATTGTCAAGTAATCTCTCTCTCTCTCTTTCTTGCTTAGTTTGCATTTTACCCTCTCTTATTTTCTAGGAAAAACACATTTCAATCCTAGATTTGTTTTTTGTTCAGTTTCAAATGGTCCACATATATTCACAAAATAATATGTTGCTATTTTAGTTTTCAACAACTTACATTTTTATTACAAATCAATCCATTGAATTCAAAACATTAAAATGTTAGAGTGGTTTTTATTTCAACTTAATTAATCTAAGATTTATACTTTACTTAAATTTTTATTAAATACACAGGCTTTTACCGTTCAGGGTAAATGTCTATGCTAATTCATTTCTAAGAACTGACAGAATGATATTTAATTAAAATTTTCACTACATTATGATTTTGACTAAACTAATAGATTTTGATGTAAAAGATAAAAGTGAGTACTTAGAGAGATTTGAAAATGCAAAATTGATTTAAAGTGGCTTGTATTCTTCTTTATTAATTTCCTATATAGGCCCTGGGTCCTGTTTTCAATCTTATAAATAAAATGAGTTTGATGTAGCCAGAAAAGATGTGCATCATGTGATCCCAAACAAGTATAGAAAGCTACAAACAACAAGATCTTGGGACTTCATTGGATTGTCTTCTAATGCAAGAAGAAGTACCAAACATGAGAGTGACATTATCGTGGGCTTATTCGATACCGGTAATTCTTCTCTATTCTTCTGAATATTTTCATTCATATTTTATTAACTCATTCAATTAATTGTAGGGATCACTCCAACGGCGGATAGCTTTAAAGATGATGGCTTCGGTCCTCCACCTAAAAAATGGAAAGGCACATGTCACCACTTTGCCAATTTCACTGCCTGCAATAAGTAATTTTCCAATATTTACTTTTATAACTACCCATTATTTCTTTTTTTCTTTTTATATTTTGCTTCAATGAGATGAGAAATCAAACACATTATACAAATTTGAACTTTTGATCAAAGTAGTTCATTTGTTAAATTAATCATTTGAATTGTTTTAGTACAATGATGAATAAAAGATTATATCAATTCCTGTTCATTTAAGTTCATTTTCAACTTTCCTTGTTTTCCTTTTGTTTTGTTTTTTTTTTATTTTCTGATGTCAATCTCTTATATCATCAAGAAAACTTATCGGAGCAAGATATTTCAAGCTCGACGGCAATCCCGATCCCTCCGACATCTTGTCCCCGGTAGACACTGATGGCCATGGCACCCATACATCATCAACCGCAACGGGCAATGCAATTGCAGGGGCCAGCCTCTCTGGTCTGGCCGAAGGAACTGCCCGTGGTGGCGTGCCATCCGCGAGAGTTGCCATGTATAAGGTCTGCTGGACTAGTAGCGGGTGCTCCGATATGGATATTCTGGCCGCCTTTGACGCTGCTATACAGGATGGTGTTGATGTCATTTCGATATCAATCGGGGGAGGTGGCTTCAACAATTACTCCGATGACTCGATTTCCATCGGCGCATTTCATGCCATGAAGAAGGGTATCATCACTGTGACCTCCGCCGGGAACGGTGGTCCGACGGCCGGCAGTGTAGTCAACCACGCGCCTTGGATTGTGACGGTTGCTGCCAGTTCCATTGATCGGAAATTTATCAGCCCATTAGAGTTGGGCAATGGGAAGAACATCTCTGTAAGTTTATTGCATCATTATGTTTTTGCATGTTCTCTCTTTTCTTTTTTGGTTGGATCAATATCAATATTATCAAATATATTTTTTAATTATAATTAATGTTAAATATTTCATAAATTTAATTAGATTGAAATTAATTAAAGTTCAGAGGTTTATTTGTTATTTTTTTTTTTTTTGTTAAAGTTTAAGATAATCTATTTTAAATTTACTAATATATCAAACCCCACCAGTAAACTAAAATAAGAAACATTTTGAGAGTATGTATACATCAAAATGAATAAAATAACAATATAAAGACGAAATTAGTATTTAAATCAAAATAACTAAGATTTTTTTTTTAAATAGTAGATTTTACAAAATATTTATAACTTATAGCAAATTCTGTCGATGATAGTCATTGATATGCTATAGTGATAGAAGACTATCAATGATAATCCAAATTTTTGCTACAACTTGTAAATATTTTAATTTATTTTGCTATTTTAAAAAATACTTCAATACCTAAAGTATTATATTATTTTATTATTAAATCAAATGTATTTAATTCTTTTTTCTTTATATTTTAATATAAAAATTAAATCAAAATATAGTAAAATATTAGTCTATATACAATGATTTAGTACTGTAGATTAAGATAATAGATTACTATTTGTGTATTTTGTAATAATAGGATGCATGCAAAAATGGTAGATATTATATTTATATGTTATACTAATTTTTTTTTATTTTAACTTTGTACTTGCTTATTTGTTTTGTATGAGTAATAATATAATAATAATGGAGATATAGGGAGTAGGGATAAACATCTTCAATCCAAAGCAAAAGATGTACCCATTAGTGAGTGGAGGGGATGTGGCAAGGAATTCAGAGAGCAAAGATACTGCAAGCTTTTGTCTAGAAGGCACACTTGATCCAACCAAAGTGAAAGGCAGCCTTGTTTTTTGCAAATTGTTAACTTGGGGAGCTGATTCTGTTATAAAATCCATTGGAGCAAATGGTGTTATCATTCAAAGTGATGAGTTTCTTGATAATGCTGATATCTTCATGGCCCCTGCCACCATGGTTAGCAGCTTGGTTGGTAATATTATTTACACCTACATCAAGTCCACCAGGTAAATGCATTCATACACACAATCAATTCCATCATTTTCATCTTAAGACCATTTATATTTAGAGAGAAGTTTTATCTTAATTCTTTTTAATCTGGCCCTTTGAGATCGTGTTGGCCTCTTTGAGTTAACTTATTCTTGAGAATGAATAATGATCTGAATTTTTTAAAATTGAAAACACTGATATCAGATCTAGATAAACGTGTAGTTTTATAAATGTGAAAAGAGTCCATTTTTTCAATCCACCAATGTTGCTTTTGAAAACAGAACCCCAACTGCAGTGATATATAAAACAAAACAACTCAAAGCAAAAGCTCCAATGGTGGCTTCCTTCTCATCTAGAGGCCCAAACCCAGGCTCCCATCGCATTCTAAAGGTTAGGTTAAGTTCAGTTTCCATCTTTTAATATTATCATTTATGATTATTCAAAATTAATTTCATATTTTTAAATAAAAACTTTGATTGTTATGGAGGTGTTAGAATTGTTGATATAATACTAAATTTACCATAACTCATTAAAAAAAAAAAAGTAGTACTTTATGATTAATAATTGAATGTATACCCACATTTTTAAAAAATTGCAGATGGTACCAAAAGCTTACCTGTGATATGGTGTATAACTGATAGAGGATCTAAATTATCTCATATATATATATTCTTTTATTTATTTATATATATATATTTTTGGCATTATTCTATTTGCAATTGTGGTTTTAATTTATTTATTTTTGTCAAATAGATTGATGTTTTTAGTCTAAAATGAAAAATGATAGTAATTGGCACACATTTGGAACTCAGGAGGTGAACAAATATAATAGTGAGGTAGGATGATAAATTTATTAAACACAAAAGTAAGAGCTGAAATATACGATTTTGTCCATTATCAGCCGGATATAGCAGCGCCAGGAGTGGACATATTGGCTGCTTATACGCCATTGAAGTCATTGACAGGGCAAAAGGGTGACACTCAATACTCCAAATTCACACTCATGTCCGGCACTTCCATGGCCTGCCCCCATGTCGCCGCTGCTGCCGCCTACGTCAAATCTTTCCATCCTCTTTGGTCTCCCGCCGCCATCAGATCCGCCTTGCTCACCACCGGTACCACCTCCCTCTCCCTTCAACTTCTTTTATTTTAACTCACTCAACCTTGAAAAGCTTTAAAACTTACATTCAAATGAATAATTCATTTTTTTATTTAAATCAGCTAGTGGTGTTGAGTAACCATTTTGTTTTTTTATTTTTGAACATGTAAAATCAATCGTCTGGTTTATTGTTTTTCAAAAGTAAGCTTATAAGACACCATTAAAAAAATTAGGTGGAAGTAAGGTTAGGGTTGAGAAACAAAAAATATAGAATGAGCCTAAATTAAAAAAAATTCTTCCAATGCTTTACGGTTATGGAAGTTTATTCTCTAAAATTTAACAATTTTGATTTGTATTGTGAAGCGACACCGATTAGCCGACGACTGAACCCTGAGGGTGAATTTGCCTATGGTGCTGGCAACCTAAACCCATCAAGAGCCATAAGTCCTGGCTTAATCTACGATCTCAATGAAATCTCGTATATCCAATTCCTTTGCAGCGAAGGGTACACTGGATCTTCCATTGCTGTCCTCTCTGGAACCAAATCCATAAACTGTTCCAATCTAATCCCCGGCCAAGGACACGACTCACTCAATTATCCAACTTTTCAGCTCAGTCTCAAAAGCACCAACCAACCGATGACAACAACGTTCCGACGACGAGTCACAAACGTCGGCCATCCTATCTCTGTCTACAATGCGACCATCAATGCTCCTCCTGGAGTGACGATCACGGTTACGCCCCCCACTCTATCATTTTCACGGTTACTTCAGAAGAGGAGTTTTAAAGTTGTTGTGAAGGCCAGTCCTTTGCCATCGGCAAAAATGGTGTCCGGATCACTTGCTTGGGTTGGTGCTCAACATGTTGTGAGAAGTCCTATTGTTGTTTATAGTCCATGA

mRNA sequence

ATCTTCTCGTCTTCTTGTCTTTGCTCTTTTCATCGTCGTAGGATGCGTTGCAGGGTTGGACGAGGACGAGGAAAAGAATCATTATATTGTATTCTTGGAAAATAAACCAGTTTTAAATGAGGTTGATGTAGTAGAAACACATCTAAATCTTCTCATGTCAGTGAAGAAAAGCCATGCCGAAGCAAGTGAATCGATGGTTTATAGTTACACGAAAAGCTTCAATGCCTTTGCTGCTAAACTTAGTGATGATGAAGCCAAATTATTGTCAACCAGAAAAGATGTGCATCATGTGATCCCAAACAAGTATAGAAAGCTACAAACAACAAGATCTTGGGACTTCATTGGATTGTCTTCTAATGCAAGAAGAAGTACCAAACATGAGAGTGACATTATCGTGGGCTTATTCGATACCGGGATCACTCCAACGGCGGATAGCTTTAAAGATGATGGCTTCGGTCCTCCACCTAAAAAATGGAAAGGCACATGTCACCACTTTGCCAATTTCACTGCCTGCAATAAAAAACTTATCGGAGCAAGATATTTCAAGCTCGACGGCAATCCCGATCCCTCCGACATCTTGTCCCCGGTAGACACTGATGGCCATGGCACCCATACATCATCAACCGCAACGGGCAATGCAATTGCAGGGGCCAGCCTCTCTGGTCTGGCCGAAGGAACTGCCCGTGGTGGCGTGCCATCCGCGAGAGTTGCCATGTATAAGGTCTGCTGGACTAGTAGCGGGTGCTCCGATATGGATATTCTGGCCGCCTTTGACGCTGCTATACAGGATGGTGTTGATGTCATTTCGATATCAATCGGGGGAGGTGGCTTCAACAATTACTCCGATGACTCGATTTCCATCGGCGCATTTCATGCCATGAAGAAGGGTATCATCACTGTGACCTCCGCCGGGAACGGTGGTCCGACGGCCGGCAGTGTAGTCAACCACGCGCCTTGGATTGTGACGGTTGCTGCCAGTTCCATTGATCGGAAATTTATCAGCCCATTAGAGTTGGGCAATGGGAAGAACATCTCTGGAGTAGGGATAAACATCTTCAATCCAAAGCAAAAGATGTACCCATTAGTGAGTGGAGGGGATGTGGCAAGGAATTCAGAGAGCAAAGATACTGCAAGCTTTTGTCTAGAAGGCACACTTGATCCAACCAAAGTGAAAGGCAGCCTTGTTTTTTGCAAATTGTTAACTTGGGGAGCTGATTCTGTTATAAAATCCATTGGAGCAAATGGTGTTATCATTCAAAGTGATGAGTTTCTTGATAATGCTGATATCTTCATGGCCCCTGCCACCATGGTTAGCAGCTTGGTTGGTAATATTATTTACACCTACATCAAGTCCACCAGAACCCCAACTGCAGTGATATATAAAACAAAACAACTCAAAGCAAAAGCTCCAATGGTGGCTTCCTTCTCATCTAGAGGCCCAAACCCAGGCTCCCATCGCATTCTAAAGCCGGATATAGCAGCGCCAGGAGTGGACATATTGGCTGCTTATACGCCATTGAAGTCATTGACAGGGCAAAAGGGTGACACTCAATACTCCAAATTCACACTCATGTCCGGCACTTCCATGGCCTGCCCCCATGTCGCCGCTGCTGCCGCCTACGTCAAATCTTTCCATCCTCTTTGGTCTCCCGCCGCCATCAGATCCGCCTTGCTCACCACCGCGACACCGATTAGCCGACGACTGAACCCTGAGGGTGAATTTGCCTATGGTGCTGGCAACCTAAACCCATCAAGAGCCATAAGTCCTGGCTTAATCTACGATCTCAATGAAATCTCGTATATCCAATTCCTTTGCAGCGAAGGGTACACTGGATCTTCCATTGCTGTCCTCTCTGGAACCAAATCCATAAACTGTTCCAATCTAATCCCCGGCCAAGGACACGACTCACTCAATTATCCAACTTTTCAGCTCAGTCTCAAAAGCACCAACCAACCGATGACAACAACGTTCCGACGACGAGTCACAAACGTCGGCCATCCTATCTCTGTCTACAATGCGACCATCAATGCTCCTCCTGGAGTGACGATCACGGTTACGCCCCCCACTCTATCATTTTCACGGTTACTTCAGAAGAGGAGTTTTAAAGTTGTTGTGAAGGCCAGTCCTTTGCCATCGGCAAAAATGGTGTCCGGATCACTTGCTTGGGTTGGTGCTCAACATGTTGTGAGAAGTCCTATTGTTGTTTATAGTCCATGA

Coding sequence (CDS)

ATGTCAGTGAAGAAAAGCCATGCCGAAGCAAGTGAATCGATGGTTTATAGTTACACGAAAAGCTTCAATGCCTTTGCTGCTAAACTTAGTGATGATGAAGCCAAATTATTGTCAACCAGAAAAGATGTGCATCATGTGATCCCAAACAAGTATAGAAAGCTACAAACAACAAGATCTTGGGACTTCATTGGATTGTCTTCTAATGCAAGAAGAAGTACCAAACATGAGAGTGACATTATCGTGGGCTTATTCGATACCGGGATCACTCCAACGGCGGATAGCTTTAAAGATGATGGCTTCGGTCCTCCACCTAAAAAATGGAAAGGCACATGTCACCACTTTGCCAATTTCACTGCCTGCAATAAAAAACTTATCGGAGCAAGATATTTCAAGCTCGACGGCAATCCCGATCCCTCCGACATCTTGTCCCCGGTAGACACTGATGGCCATGGCACCCATACATCATCAACCGCAACGGGCAATGCAATTGCAGGGGCCAGCCTCTCTGGTCTGGCCGAAGGAACTGCCCGTGGTGGCGTGCCATCCGCGAGAGTTGCCATGTATAAGGTCTGCTGGACTAGTAGCGGGTGCTCCGATATGGATATTCTGGCCGCCTTTGACGCTGCTATACAGGATGGTGTTGATGTCATTTCGATATCAATCGGGGGAGGTGGCTTCAACAATTACTCCGATGACTCGATTTCCATCGGCGCATTTCATGCCATGAAGAAGGGTATCATCACTGTGACCTCCGCCGGGAACGGTGGTCCGACGGCCGGCAGTGTAGTCAACCACGCGCCTTGGATTGTGACGGTTGCTGCCAGTTCCATTGATCGGAAATTTATCAGCCCATTAGAGTTGGGCAATGGGAAGAACATCTCTGGAGTAGGGATAAACATCTTCAATCCAAAGCAAAAGATGTACCCATTAGTGAGTGGAGGGGATGTGGCAAGGAATTCAGAGAGCAAAGATACTGCAAGCTTTTGTCTAGAAGGCACACTTGATCCAACCAAAGTGAAAGGCAGCCTTGTTTTTTGCAAATTGTTAACTTGGGGAGCTGATTCTGTTATAAAATCCATTGGAGCAAATGGTGTTATCATTCAAAGTGATGAGTTTCTTGATAATGCTGATATCTTCATGGCCCCTGCCACCATGGTTAGCAGCTTGGTTGGTAATATTATTTACACCTACATCAAGTCCACCAGAACCCCAACTGCAGTGATATATAAAACAAAACAACTCAAAGCAAAAGCTCCAATGGTGGCTTCCTTCTCATCTAGAGGCCCAAACCCAGGCTCCCATCGCATTCTAAAGCCGGATATAGCAGCGCCAGGAGTGGACATATTGGCTGCTTATACGCCATTGAAGTCATTGACAGGGCAAAAGGGTGACACTCAATACTCCAAATTCACACTCATGTCCGGCACTTCCATGGCCTGCCCCCATGTCGCCGCTGCTGCCGCCTACGTCAAATCTTTCCATCCTCTTTGGTCTCCCGCCGCCATCAGATCCGCCTTGCTCACCACCGCGACACCGATTAGCCGACGACTGAACCCTGAGGGTGAATTTGCCTATGGTGCTGGCAACCTAAACCCATCAAGAGCCATAAGTCCTGGCTTAATCTACGATCTCAATGAAATCTCGTATATCCAATTCCTTTGCAGCGAAGGGTACACTGGATCTTCCATTGCTGTCCTCTCTGGAACCAAATCCATAAACTGTTCCAATCTAATCCCCGGCCAAGGACACGACTCACTCAATTATCCAACTTTTCAGCTCAGTCTCAAAAGCACCAACCAACCGATGACAACAACGTTCCGACGACGAGTCACAAACGTCGGCCATCCTATCTCTGTCTACAATGCGACCATCAATGCTCCTCCTGGAGTGACGATCACGGTTACGCCCCCCACTCTATCATTTTCACGGTTACTTCAGAAGAGGAGTTTTAAAGTTGTTGTGAAGGCCAGTCCTTTGCCATCGGCAAAAATGGTGTCCGGATCACTTGCTTGGGTTGGTGCTCAACATGTTGTGAGAAGTCCTATTGTTGTTTATAGTCCATGA

Protein sequence

MSVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIGLSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACNKKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPKQKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIGANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMVASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLIYDLNEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSAKMVSGSLAWVGAQHVVRSPIVVYSP*
BLAST of Cucsa.019390 vs. Swiss-Prot
Match: SBT4E_ARATH (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 1.4e-255
Identity = 430/686 (62.68%), Postives = 530/686 (77.26%), Query Frame = 1

Query: 2   SVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWD 61
           S+  S  EA E  VYSYTK+FNAFAAKLS  EAK +   ++V  V  N+YRKL TT+SWD
Sbjct: 62  SLNISQEEAKERKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWD 121

Query: 62  FIGLSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACN 121
           F+GL   A+R  K E D+I+G+ DTGITP ++SF D G GPPP KWKG+C  + NFT CN
Sbjct: 122 FVGLPLTAKRHLKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCN 181

Query: 122 KKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVP 181
            K+IGA+YFK DGN    ++ SP+D DGHGTHTSST  G  +A ASL G+A GTARG VP
Sbjct: 182 NKIIGAKYFKHDGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVP 241

Query: 182 SARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHA 241
           SAR+AMYKVCW  SGC+DMDILA F+AAI DGV++ISISIGG    +YS DSIS+G+FHA
Sbjct: 242 SARLAMYKVCWARSGCADMDILAGFEAAIHDGVEIISISIGGP-IADYSSDSISVGSFHA 301

Query: 242 MKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIF 301
           M+KGI+TV SAGN GP++G+V NH PWI+TVAAS IDR F S ++LGNGK+ SG+GI++F
Sbjct: 302 MRKGILTVASAGNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMF 361

Query: 302 NPKQKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIG 361
           +PK K YPLVSG D A+N++ K  A +C   +LD  KVKG ++ C++   G +S IKS G
Sbjct: 362 SPKAKSYPLVSGVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYG 421

Query: 362 ANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMV 421
             G II SD++LDNA IFMAPAT V+S VG+IIY YI STR+ +AVI KT+Q+   AP V
Sbjct: 422 GAGAIIVSDQYLDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFV 481

Query: 422 ASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACP 481
           ASFSSRGPNPGS R+LKPDIAAPG+DILAA+T  +SLTG  GDTQ+SKFT++SGTSMACP
Sbjct: 482 ASFSSRGPNPGSIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACP 541

Query: 482 HVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLI 541
           HVA  AAYVKSFHP W+PAAI+SA++T+A PISRR+N + EFAYG G +NP RA SPGL+
Sbjct: 542 HVAGVAAYVKSFHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLV 601

Query: 542 YDLNEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQP 601
           YD+++ISY+QFLC EGY  +++A L GT+S++CS+++PG GHDSLNYPT QL+L+S    
Sbjct: 602 YDMDDISYVQFLCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTS 661

Query: 602 MTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPS 661
               FRRRVTNVG P SVY AT+ AP GV ITV P +LSFS+  QKRSFKVVVKA  +  
Sbjct: 662 TLAVFRRRVTNVGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTP 721

Query: 662 AKMVSGSLAWVGAQHVVRSPIVVYSP 688
            K+VSG L W   +H VRSPIV+YSP
Sbjct: 722 GKIVSGLLVWKSPRHSVRSPIVIYSP 746

BLAST of Cucsa.019390 vs. Swiss-Prot
Match: SBT4F_ARATH (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 2.6e-182
Identity = 356/705 (50.50%), Postives = 461/705 (65.39%), Query Frame = 1

Query: 1   MSVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSW 60
           M+V    ++A E  +YSY K+ N F A+L   EA+ LS  + V  V  N  R+L TTRSW
Sbjct: 59  MTVIGDESKARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSW 118

Query: 61  DFIGL-SSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTA 120
           DF+GL  S  +RS   ES+IIVG+ DTGI   + SF D G GPPP KWKG C    NFT 
Sbjct: 119 DFLGLVESKYKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTR 178

Query: 121 CNKKLIGARYFKL--DGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTAR 180
           CN K+IGA+YF +  +G PD     +  D DGHGTHTSST  G +++ ASL G+A GTAR
Sbjct: 179 CNNKVIGAKYFHIQSEGLPDGEGDTA-ADHDGHGTHTSSTIAGVSVSSASLFGIANGTAR 238

Query: 181 GGVPSARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIG 240
           GGVPSAR+A YKVCW  SGC+DMD+LAAFD AI DGVD+ISISIGG     + +D I+IG
Sbjct: 239 GGVPSARIAAYKVCW-DSGCTDMDMLAAFDEAISDGVDIISISIGGASLP-FFEDPIAIG 298

Query: 241 AFHAMKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVG 300
           AFHAMK+GI+T  SAGN GP   +V N APW++TVAA+S+DRKF + ++LGNG   SG+ 
Sbjct: 299 AFHAMKRGILTTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGIS 358

Query: 301 INIFNPKQKMYPLVSGGDVARNSESK-DTASFCLEGTLDPTKVKGSLVFCKLLT------ 360
           +N FNP++KMYPL SG   +  S       S C  GTL   KV G +V+C+         
Sbjct: 359 LNGFNPRKKMYPLTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGGNG 418

Query: 361 -WGADSVIKSIGANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIY 420
             G D V++S+   GVI+Q  E  D A   +   + V    G  I  YI ST+ P AVI+
Sbjct: 419 GQGQDHVVRSLKGAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIF 478

Query: 421 KTKQLKAKAPMVASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSK 480
           KTK  K  AP ++SFS+RGP   S  ILKPDI+APG++ILAAY+ L S+TG   D + + 
Sbjct: 479 KTKTTKMLAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTL 538

Query: 481 FTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGN 540
           F++MSGTSMACPH AAAAAYVKSFHP WSPAAI+SAL+TTATP+  + N E E +YG+G 
Sbjct: 539 FSIMSGTSMACPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGN-EAELSYGSGQ 598

Query: 541 LNPSRAISPGLIYDLNEISYIQFLCSEGYTGSSIAVLSG-------TKSINCSNLIPGQG 600
           +NP RAI PGL+YD+ E +Y++FLC EGY  +SI +L+G        K  NC N+  G G
Sbjct: 599 INPRRAIHPGLVYDITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLG 658

Query: 601 HDSLNYPTFQLSLKSTNQPMTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFS 660
            D LNYP+    + ST   ++  F R VTNVG+  S Y A + AP G+ + V P  +SF 
Sbjct: 659 SDGLNYPSLHKQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFE 718

Query: 661 RLLQKRSFKVVVKASPLPSAK-MVSGSLAWVGAQ-HVVRSPIVVY 686
           R  +KR+FKVV+      + K +VS S+ W  ++ H+VRSPI+++
Sbjct: 719 RPKEKRNFKVVIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLF 759

BLAST of Cucsa.019390 vs. Swiss-Prot
Match: CUCM1_CUCME (Cucumisin OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 2.3e-170
Identity = 316/681 (46.40%), Postives = 460/681 (67.55%), Query Frame = 1

Query: 10  ASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIGLSSNA 69
           A ES++++Y +SFN FA KL+++EA+ +++ + V  V  N+  +L TTRSWDF+G     
Sbjct: 65  APESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLTV 124

Query: 70  RRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACNKKLIGARY 129
            R ++ ES+I+VG+ DTGI P + SF D+GF PPP KWKGTC    NF  CN+K+IGAR 
Sbjct: 125 PRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFR-CNRKIIGARS 184

Query: 130 FKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSARVAMYK 189
           + +     P D+  P DT+GHGTHT+STA G  ++ A+L GL  GTARGGVP AR+A YK
Sbjct: 185 YHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYK 244

Query: 190 VCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKKGIITV 249
           VCW + GCSD DILAA+D AI DGVD+IS+S+GG    +Y  D+I+IG+FHA+++GI+T 
Sbjct: 245 VCW-NDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTS 304

Query: 250 TSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPKQKMYP 309
            SAGNGGP   +  + +PW+++VAAS++DRKF++ +++GNG++  GV IN F+   + YP
Sbjct: 305 NSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYP 364

Query: 310 LVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSI-GANGVIIQ 369
           LVSG D+      K T+ FC + +++P  +KG +V C+  ++G     KS+ GA GV++ 
Sbjct: 365 LVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEA-SFGPHEFFKSLDGAAGVLMT 424

Query: 370 SDEFLDNADIFMAPATMV--SSLVGNIIYTYIKSTRTPTAVIYK-TKQLKAKAPMVASFS 429
           S+   D AD +  P++++  + L+  + Y Y  S R+P A I+K T  L A AP+V SFS
Sbjct: 425 SNT-RDYADSYPLPSSVLDPNDLLATLRYIY--SIRSPGATIFKSTTILNASAPVVVSFS 484

Query: 430 SRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACPHVAA 489
           SRGPN  +  ++KPDI+ PGV+ILAA+  +  + G + +T    F ++SGTSM+CPH+  
Sbjct: 485 SRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTL---FNIISGTSMSCPHITG 544

Query: 490 AAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLIYDLN 549
            A YVK+++P WSPAAI+SAL+TTA+P++ R NP+ EFAYG+G++NP +A+ PGL+YD N
Sbjct: 545 IATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDAN 604

Query: 550 EISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTTT 609
           E  Y++FLC +GY   ++  ++G  S  C++   G+  D LNYP+F LS+ S +Q     
Sbjct: 605 ESDYVKFLCGQGYNTQAVRRITGDYSA-CTSGNTGRVWD-LNYPSFGLSV-SPSQTFNQY 664

Query: 610 FRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSAKMV 669
           F R +T+V    S Y A I+AP G+TI+V P  LSF+ L  ++SF + V+ S      +V
Sbjct: 665 FNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGS--IKGFVV 724

Query: 670 SGSLAWVGAQHVVRSPIVVYS 687
           S SL W    H VRSPI + S
Sbjct: 725 SASLVWSDGVHYVRSPITITS 729

BLAST of Cucsa.019390 vs. Swiss-Prot
Match: SBT43_ARATH (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 3.9e-170
Identity = 324/681 (47.58%), Postives = 442/681 (64.90%), Query Frame = 1

Query: 10  ASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIGLSSNA 69
           AS  +V SY +SFN FAA LS  E++ L   K+V  V P+K  +L TTRSWDF+G    A
Sbjct: 64  ASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKA 123

Query: 70  RRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACNKKLIGARY 129
           RR +  ESD+IVG+ D+GI P ++SF D+GFGPPPKKWKG+C     F ACN KLIGAR+
Sbjct: 124 RRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKF-ACNNKLIGARF 183

Query: 130 FKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSARVAMYK 189
           +    +       S  D +GHGTHT+STA GNA+  AS  GLA+GTARGGVPSAR+A YK
Sbjct: 184 YNKFAD-------SARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYK 243

Query: 190 VCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKKGIITV 249
           VC+    C+D+DILAAFD AI DGVDVISISI     +N  + S++IG+FHAM +GIIT 
Sbjct: 244 VCFNR--CNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITA 303

Query: 250 TSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPKQKMYP 309
            SAGN GP  GSV N +PW++TVAAS  DR+FI  + LGNGK ++G+ +N FN     +P
Sbjct: 304 GSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFP 363

Query: 310 LVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIGANGVIIQS 369
           +V G +V+RN  S+  A +C  G +D   VKG +V C       ++ +   GA GVI+Q+
Sbjct: 364 IVYGQNVSRNC-SQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYL--AGAIGVIVQN 423

Query: 370 DEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQL-KAKAPMVASFSSRG 429
               D+A +   PA+ +       I +YI+S   P A I +T+++   +AP V SFSSRG
Sbjct: 424 TLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRG 483

Query: 430 PNPGSHRILKPDIAAPGVDILAAYTPLKSLTG--QKGDTQYSKFTLMSGTSMACPHVAAA 489
           P+     +LKPD++APG++ILAA++P+ S +      D +  ++++MSGTSMACPHVA  
Sbjct: 484 PSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGV 543

Query: 490 AAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLIYDLNE 549
           AAYVKSFHP WSP+AI+SA++TTATP++ + NPE EFAYG+G +NP++A  PGL+Y++  
Sbjct: 544 AAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVET 603

Query: 550 ISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTTTF 609
             Y++ LC+EG+  +++   SG +++ CS     +    LNYPT    + S + P   TF
Sbjct: 604 EDYLKMLCAEGFDSTTLTTTSG-QNVTCSERTEVK---DLNYPTMTTFVSSLD-PFNVTF 663

Query: 610 RRRVTNVGHPISVYNAT-INAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSAKMV 669
           +R VTNVG P S Y A+ +   P + I++ P  L F  L +K+SF V +    L     V
Sbjct: 664 KRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFV 723

Query: 670 SGSLAWVGAQHVVRSPIVVYS 687
           S S+ W    H VRSPIV YS
Sbjct: 724 SSSVVWSDGSHSVRSPIVAYS 726

BLAST of Cucsa.019390 vs. Swiss-Prot
Match: SBT46_ARATH (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 3.0e-162
Identity = 312/684 (45.61%), Postives = 435/684 (63.60%), Query Frame = 1

Query: 12  ESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIGLSSNAR- 71
           + +V +Y +SFN FAA+L++ E ++L++  +V  V P+K   LQTT SW+F+GL    R 
Sbjct: 68  DRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRT 127

Query: 72  -RSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACNKKLIGARY 131
            R+   ESD I+G+ D+GI P +DSF   GFGPPPKKWKG C    NFT CN KLIGARY
Sbjct: 128 KRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFT-CNNKLIGARY 187

Query: 132 F--KLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSARVAM 191
           +  KL+G P+     S  D  GHG+HT+S A GNA+   S  GL  GT RGGVP+AR+A+
Sbjct: 188 YTPKLEGFPE-----SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAV 247

Query: 192 YKVCWTSS-GCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKKGI 251
           YKVC      C+   ILAAFD AI D VD+I++S+G      + +D+++IGAFHAM KGI
Sbjct: 248 YKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGI 307

Query: 252 ITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPKQK 311
           +TV  AGN GP   ++V+ APW+ TVAAS+++R FI+ + LGNGK I G  +N F+   K
Sbjct: 308 LTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGK 367

Query: 312 MYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIGANGVI 371
            YPLV G   +   ++  +A FC  G LD  +VKG +V C   T       +++GA   I
Sbjct: 368 KYPLVYGKSASSRCDA-SSAGFCSPGCLDSKRVKGKIVLCD--TQRNPGEAQAMGAVASI 427

Query: 372 IQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQL-KAKAPMVASFS 431
           ++ + + D A +F  P +++S    NI+ +Y+ ST+ P A + K++ +   KAP+VAS+S
Sbjct: 428 VR-NPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYS 487

Query: 432 SRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACPHVAA 491
           SRGPNP  H ILKPDI APG +ILAAY+P   +   + DT++ K+T++SGTSM+CPHVA 
Sbjct: 488 SRGPNPLIHDILKPDITAPGSEILAAYSPY--VPPSESDTRHVKYTVISGTSMSCPHVAG 547

Query: 492 AAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPE---GEFAYGAGNLNPSRAISPGLIY 551
            AAY+K+FHPLWSP+ I+SA++TTA P++   +P     EFAYGAG+++P  AI PGL+Y
Sbjct: 548 VAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVY 607

Query: 552 DLNEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPM 611
           + N+  +I FLC   YTG  + ++SG  S +C+         +LNYP+    +  T +P 
Sbjct: 608 EANKSDHITFLCGFNYTGKKLRLISGDSS-SCTKEQTKSLTRNLNYPSMSAQVSGT-KPF 667

Query: 612 TTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSA 671
             TFRR VTNVG P + Y A +     + + V P  LS   L +K+SF V V  +   + 
Sbjct: 668 KVTFRRTVTNVGRPNATYKAKV-VGSKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAE 727

Query: 672 KMVSGSLAWVGAQHVVRSPIVVYS 687
            +VS  L W    H VRSPIVVY+
Sbjct: 728 NLVSAQLIWSDGVHFVRSPIVVYA 736

BLAST of Cucsa.019390 vs. TrEMBL
Match: A0A0A0KME6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G000010 PE=4 SV=1)

HSP 1 Score: 1372.8 bits (3552), Expect = 0.0e+00
Identity = 687/687 (100.00%), Postives = 687/687 (100.00%), Query Frame = 1

Query: 1   MSVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSW 60
           MSVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSW
Sbjct: 57  MSVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSW 116

Query: 61  DFIGLSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTAC 120
           DFIGLSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTAC
Sbjct: 117 DFIGLSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTAC 176

Query: 121 NKKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGV 180
           NKKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGV
Sbjct: 177 NKKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGV 236

Query: 181 PSARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFH 240
           PSARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFH
Sbjct: 237 PSARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFH 296

Query: 241 AMKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINI 300
           AMKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINI
Sbjct: 297 AMKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINI 356

Query: 301 FNPKQKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSI 360
           FNPKQKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSI
Sbjct: 357 FNPKQKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSI 416

Query: 361 GANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPM 420
           GANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPM
Sbjct: 417 GANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPM 476

Query: 421 VASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMAC 480
           VASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMAC
Sbjct: 477 VASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMAC 536

Query: 481 PHVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGL 540
           PHVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGL
Sbjct: 537 PHVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGL 596

Query: 541 IYDLNEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQ 600
           IYDLNEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQ
Sbjct: 597 IYDLNEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQ 656

Query: 601 PMTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLP 660
           PMTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLP
Sbjct: 657 PMTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLP 716

Query: 661 SAKMVSGSLAWVGAQHVVRSPIVVYSP 688
           SAKMVSGSLAWVGAQHVVRSPIVVYSP
Sbjct: 717 SAKMVSGSLAWVGAQHVVRSPIVVYSP 743

BLAST of Cucsa.019390 vs. TrEMBL
Match: M5X7G0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021240mg PE=4 SV=1)

HSP 1 Score: 975.3 bits (2520), Expect = 3.8e-281
Identity = 476/687 (69.29%), Postives = 563/687 (81.95%), Query Frame = 1

Query: 2   SVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWD 61
           SVK+S+ EA ESMVYSYTKSFNAFAAKLS DEA+ LS   +V  V+PN+Y KL TT+SWD
Sbjct: 66  SVKESYVEAEESMVYSYTKSFNAFAAKLSSDEAERLSGMDEVLSVLPNRYHKLHTTKSWD 125

Query: 62  FIGLSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACN 121
           FIGL   ARR+ K E DIIVGLFDTGITP + SFK DGFGPPP KWKGTC HFANF+ CN
Sbjct: 126 FIGLPLKARRNLKLERDIIVGLFDTGITPESKSFKGDGFGPPPAKWKGTCGHFANFSGCN 185

Query: 122 KKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVP 181
            K+IGA+YFKLDGNPDP+DILSP+D DGHGTHTSST  GN +  ASL GLA GTARG VP
Sbjct: 186 NKIIGAKYFKLDGNPDPTDILSPIDVDGHGTHTSSTLAGNQVPNASLFGLAMGTARGAVP 245

Query: 182 SARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHA 241
           SARVAMYKVCW SSGC+DMDILAA+DAAI DGVDVIS SI GG   NY  DS++IGAFHA
Sbjct: 246 SARVAMYKVCWASSGCTDMDILAAYDAAIHDGVDVISFSISGG-VANYVRDSVAIGAFHA 305

Query: 242 MKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIF 301
           MKKGIITV SAGN GP+ GSVVNHAPW VTVAAS IDR+FIS + LGNGK++SG+G+N F
Sbjct: 306 MKKGIITVASAGNDGPSLGSVVNHAPWFVTVAASGIDRQFISKVHLGNGKDVSGIGVNTF 365

Query: 302 NPKQKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIG 361
           NPK+ +YPLVSG DVARNS++K++A FC++ +LDP KVKG LV CKL  WGADSV+K IG
Sbjct: 366 NPKKNLYPLVSGVDVARNSQTKESARFCVQDSLDPNKVKGKLVLCKLENWGADSVVKGIG 425

Query: 362 ANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMV 421
             G II+ D+ LD A IFMAP TMV+  +  II  YI STR+P+AVI K+++ K +AP +
Sbjct: 426 GVGTIIEDDQLLDAAQIFMAPGTMVNDTIAEIIDEYIHSTRSPSAVISKSQEAKKRAPFI 485

Query: 422 ASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACP 481
           ASFSSRGPNPGS RILKPDIAAPGVDILA+YT  KSLTG +GDTQYS+FTLMSGTSM+CP
Sbjct: 486 ASFSSRGPNPGSERILKPDIAAPGVDILASYTLRKSLTGLEGDTQYSEFTLMSGTSMSCP 545

Query: 482 HVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLI 541
           HVA   AYVKSFHP WSPAA+RSA+LTTA P+S+R N + E +YG G +NP++A++PGL+
Sbjct: 546 HVAGVVAYVKSFHPHWSPAALRSAILTTAKPMSQRGNSDAELSYGVGQVNPTKAVNPGLV 605

Query: 542 YDLN-EISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQ 601
           YD++  +SYIQFLC EGY GSS+A L G+KS+NCS+L+PGQG+D+LNYPT QLSLKS+ Q
Sbjct: 606 YDMDGTLSYIQFLCHEGYKGSSLAPLVGSKSVNCSSLLPGQGYDALNYPTMQLSLKSSKQ 665

Query: 602 PMTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLP 661
           P    FRR VTNVG   S YNATI AP GV I V P +LSFS  L+KR+F+VVVKA+P+P
Sbjct: 666 PTVGVFRRTVTNVGPAKSTYNATIRAPKGVEIIVKPMSLSFSTTLEKRTFQVVVKANPMP 725

Query: 662 SAKMVSGSLAWVGAQHVVRSPIVVYSP 688
           S +M+SGSL W  +++ VRSPIV++SP
Sbjct: 726 SMRMISGSLGWKSSRYTVRSPIVIHSP 751

BLAST of Cucsa.019390 vs. TrEMBL
Match: A0A067HDF0_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g004503mg PE=4 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 5.5e-280
Identity = 470/682 (68.91%), Postives = 561/682 (82.26%), Query Frame = 1

Query: 6   SHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIGL 65
           S+ +A ES+VYSYT+SFNAFAAKLS+DEA+ L     V  V PN+Y +L TTRSWDFIGL
Sbjct: 66  SYHDAKESIVYSYTESFNAFAAKLSNDEAQKLQRMDRVLSVFPNRYHQLHTTRSWDFIGL 125

Query: 66  SSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACNKKLI 125
              ARR+ K ESDI+VGL DTGITP ++SFKD GFGPPP KWKG C HFANF+ CN KLI
Sbjct: 126 PQTARRNLKIESDIVVGLMDTGITPESESFKDSGFGPPPAKWKGKCDHFANFSGCNNKLI 185

Query: 126 GARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSARV 185
           GARYFKLDGNPDP DILSP+D DGHGTHTSST  GN +A ASL GLA G ARG VP+ARV
Sbjct: 186 GARYFKLDGNPDPWDILSPIDVDGHGTHTSSTLAGNVVANASLYGLAWGAARGAVPNARV 245

Query: 186 AMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKKG 245
           A YKVCW SSGCSDMDILAAFDAAI DGV+VISISIGG    +Y+ D+IS+GAFHA+KKG
Sbjct: 246 AAYKVCWVSSGCSDMDILAAFDAAIHDGVNVISISIGGAT-EDYASDTISVGAFHALKKG 305

Query: 246 IITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPKQ 305
           I+TV SAGN GP  G+V NHAPW+VTVAAS IDR+F S ++ GNG+++SGVG+N F+PK+
Sbjct: 306 IVTVASAGNDGPKWGTVSNHAPWLVTVAASGIDRQFKSKVKTGNGRSVSGVGVNTFDPKE 365

Query: 306 KMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIGANGV 365
           K YPLVSG DVA+NSES+D+A FC + +LDP KVKG LV+CKL TWGADSVIK IG  G+
Sbjct: 366 KFYPLVSGADVAKNSESRDSARFCFDDSLDPKKVKGKLVYCKLGTWGADSVIKGIGGVGI 425

Query: 366 IIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMVASFS 425
           I+ S++FLD A I+MAP TMV+   G+ I  YI STR+P+AVIYK++++K +AP +ASFS
Sbjct: 426 IVGSEQFLDVAQIYMAPGTMVNVTDGDNITDYIHSTRSPSAVIYKSQEVKVRAPFIASFS 485

Query: 426 SRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACPHVAA 485
           SRGPNPGS  +LKPDIAAPG+DILA+YT +KSLTG KGDTQYSKFTLMSGTSMACPH+A 
Sbjct: 486 SRGPNPGSKHLLKPDIAAPGIDILASYTLMKSLTGLKGDTQYSKFTLMSGTSMACPHIAG 545

Query: 486 AAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLIYDLN 545
             AYVKSFHP WSPAAI+SA++TTA P+S+R+N E EFAYGAG +NP +A+SPGL+YD++
Sbjct: 546 VVAYVKSFHPSWSPAAIKSAIMTTAKPMSQRVNNEAEFAYGAGQVNPQKAVSPGLVYDMD 605

Query: 546 EISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTTT 605
           ++SYIQFLC EGY GSS+AVL G+KSINC++LIPG G+D+LNYPT Q+SLKS  +  T  
Sbjct: 606 DMSYIQFLCHEGYNGSSLAVLVGSKSINCTSLIPGVGYDALNYPTMQVSLKSNGELTTAI 665

Query: 606 FRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSAKMV 665
           FRRRVTNVG  +S+YNATI AP GV ITV P +LSFSR   KRSF VVVKA P+ S +++
Sbjct: 666 FRRRVTNVGPRLSIYNATIKAPKGVNITVKPMSLSFSRTSHKRSFSVVVKAKPMSSTQVL 725

Query: 666 SGSLAWVGAQHVVRSPIVVYSP 688
           SGSL W   +HVVRSPIV+Y P
Sbjct: 726 SGSLEWKSPRHVVRSPIVIYRP 746

BLAST of Cucsa.019390 vs. TrEMBL
Match: B9RDI2_RICCO (Xylem serine proteinase 1, putative OS=Ricinus communis GN=RCOM_1613010 PE=4 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 2.6e-277
Identity = 473/686 (68.95%), Postives = 554/686 (80.76%), Query Frame = 1

Query: 2   SVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWD 61
           SVK+S  +A +S+VYSYTKSFNAFAAKLS  EA  LS+   V  V PN+Y KL TT+SWD
Sbjct: 59  SVKRSDDDAVDSIVYSYTKSFNAFAAKLSKAEATKLSSLDQVLSVFPNRYHKLHTTKSWD 118

Query: 62  FIGLSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACN 121
           FIGL + ARR  K E DIIVGL DTGITP ++SFK DGFGPPPKKWKGTC  FANF+ CN
Sbjct: 119 FIGLPNTARRKLKMERDIIVGLLDTGITPQSESFKGDGFGPPPKKWKGTCGRFANFSGCN 178

Query: 122 KKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVP 181
            KLIGARYFKLDGNPDP+DILSPVD DGHGTHTSST  GN I  ASL GLA+G ARG VP
Sbjct: 179 NKLIGARYFKLDGNPDPNDILSPVDVDGHGTHTSSTLAGNEIPDASLFGLAKGAARGAVP 238

Query: 182 SARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHA 241
           ++RVAMYKVCW SSGCSDMDILAAF+AAI DGVDVIS+SIGG   + Y+ D+ +IGAFHA
Sbjct: 239 ASRVAMYKVCWASSGCSDMDILAAFEAAINDGVDVISVSIGGATAD-YATDTFAIGAFHA 298

Query: 242 MKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIF 301
           M+KGIITV SAGN GP +G+V NHAPW++TVAAS IDR+F + + LGNGK +SGVG+N F
Sbjct: 299 MRKGIITVASAGNDGPMSGTVANHAPWLLTVAASGIDRQFRNKVVLGNGKTVSGVGVNAF 358

Query: 302 NPKQKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIG 361
            P QK+YPLVSG D A NS SK  A FCL+ ++D  KVKG LV+C+L  WG+DSV+K IG
Sbjct: 359 EPNQKLYPLVSGADAATNSASKSRARFCLDESMDSNKVKGKLVYCELQMWGSDSVVKGIG 418

Query: 362 ANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMV 421
             G II+S ++LD A IFM P TMV+  VG+ I  YI ST++P+AVIY++ ++K  AP +
Sbjct: 419 GVGAIIESAQYLDAAQIFMTPGTMVNVTVGDTINDYIHSTKSPSAVIYRSHEVKIPAPFI 478

Query: 422 ASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACP 481
           ASFSSRGPNPGS  +LKPDIAAPG+DILA+YTPL SLTG KGDTQYSKFTLMSGTSMACP
Sbjct: 479 ASFSSRGPNPGSKLLLKPDIAAPGIDILASYTPLHSLTGLKGDTQYSKFTLMSGTSMACP 538

Query: 482 HVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLI 541
           HVA  AAY+KSFHP WS AAI+SA+LTTA P+S R+N E EFAYGAG LNPSRA SPGL+
Sbjct: 539 HVAGVAAYIKSFHPNWSAAAIKSAILTTAKPMSARVNSEAEFAYGAGQLNPSRARSPGLV 598

Query: 542 YDLNEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQP 601
           YD++E+SYIQFLC EGYTGSS+AVL G+KSINCS+L+PG G+D++NYPT  LS ++  QP
Sbjct: 599 YDMDEMSYIQFLCHEGYTGSSLAVLIGSKSINCSSLLPGLGYDAINYPTMHLSARNDKQP 658

Query: 602 MTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPS 661
               FRR VTNVG   S YNATI AP GV ITV P +LSFSR LQKRSFKVVVKA P+ S
Sbjct: 659 TIGVFRRTVTNVGPSTSFYNATIKAPKGVEITVVPASLSFSRTLQKRSFKVVVKAKPMSS 718

Query: 662 AKMVSGSLAWVGAQHVVRSPIVVYSP 688
            +++SGS+AW  ++HVVRSPIVVY P
Sbjct: 719 GQILSGSVAWKSSRHVVRSPIVVYKP 743

BLAST of Cucsa.019390 vs. TrEMBL
Match: E6NU20_JATCU (JHL05D22.1 protein OS=Jatropha curcas GN=JHL05D22.1 PE=4 SV=1)

HSP 1 Score: 957.6 bits (2474), Expect = 8.2e-276
Identity = 470/686 (68.51%), Postives = 551/686 (80.32%), Query Frame = 1

Query: 2   SVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWD 61
           SVKKS  EA ES+VYSYTKSFNAFAAKLS  EA  LS    V  V PNKY +L TT+SWD
Sbjct: 57  SVKKSEFEAKESIVYSYTKSFNAFAAKLSKAEAAELSRLDQVLSVFPNKYHRLHTTKSWD 116

Query: 62  FIGLSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACN 121
           FIGL S ARR+ K E +I+VGL DTGITP ++SF+ DGFGPPPKKW GTC HFANFT CN
Sbjct: 117 FIGLPSKARRNLKMERNIVVGLLDTGITPESESFRGDGFGPPPKKWNGTCGHFANFTGCN 176

Query: 122 KKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVP 181
            KLIGARYFKLDGNPDP+DI SPVD DGHGTHTSST  GN I  ASL GLA G ARG VP
Sbjct: 177 NKLIGARYFKLDGNPDPNDIFSPVDVDGHGTHTSSTVAGNLIPDASLFGLARGAARGAVP 236

Query: 182 SARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHA 241
           +ARVAMYKVCW SSGCSDMDILAAF+AAI DGVDVIS+SIGG   + Y  DS++IGAFHA
Sbjct: 237 AARVAMYKVCWASSGCSDMDILAAFEAAITDGVDVISVSIGGATAD-YVSDSLAIGAFHA 296

Query: 242 MKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIF 301
           M+KGIIT  SAGN GP++G+V NHAPW++TVAAS IDR+F S +ELGNGK +SGVG+N F
Sbjct: 297 MRKGIITTASAGNDGPSSGTVANHAPWLLTVAASGIDRQFRSKIELGNGKTVSGVGVNSF 356

Query: 302 NPKQKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIG 361
             KQ++YPLVSG DVARNS +KD A FCL+G+++P+KVKG LV+C+L  WG+DSV+K IG
Sbjct: 357 ESKQQLYPLVSGADVARNSANKDNARFCLDGSMEPSKVKGKLVYCELQVWGSDSVVKGIG 416

Query: 362 ANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMV 421
             G +++S +FLD A IFM P TMV+  VG+ I  YI ST++P+AVIY++ ++K  AP V
Sbjct: 417 GIGAVVESAQFLDAAQIFMTPGTMVNVTVGDAINDYIHSTKSPSAVIYRSHEVKIPAPFV 476

Query: 422 ASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACP 481
           ASFSSRGPNP S  +LKPD+AAPG+DILA+YTPL+SLTG KGDTQYSKFTLMSGTSMA P
Sbjct: 477 ASFSSRGPNPLSEHLLKPDVAAPGIDILASYTPLRSLTGLKGDTQYSKFTLMSGTSMAGP 536

Query: 482 HVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLI 541
           HVA  AAYVKSFHP WS A I+SA+LTTA P+S R N + EFAYGAG +NP+RA +PGL+
Sbjct: 537 HVAGVAAYVKSFHPNWSAATIKSAILTTAKPMSPRANNDAEFAYGAGQVNPTRARNPGLV 596

Query: 542 YDLNEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQP 601
           YD++E+SYIQFLC EGY GSS+AVL G KSINCS+L+PG G+D+LNYPT QLS ++  QP
Sbjct: 597 YDMDEMSYIQFLCHEGYRGSSLAVLIGKKSINCSSLLPGFGYDALNYPTMQLSARNDKQP 656

Query: 602 MTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPS 661
               FRR VTNVG   S++NATI AP GV ITV P +LSFS  LQ RSFKVVVKA P+ S
Sbjct: 657 TVGVFRRTVTNVGPSPSIFNATIKAPKGVEITVEPMSLSFSHALQNRSFKVVVKAKPMSS 716

Query: 662 AKMVSGSLAWVGAQHVVRSPIVVYSP 688
            ++VSGSL W    HVVRSPIVV+ P
Sbjct: 717 GQLVSGSLVWKSFHHVVRSPIVVFKP 741

BLAST of Cucsa.019390 vs. TAIR10
Match: AT4G00230.1 (AT4G00230.1 xylem serine peptidase 1)

HSP 1 Score: 883.6 bits (2282), Expect = 7.6e-257
Identity = 430/686 (62.68%), Postives = 530/686 (77.26%), Query Frame = 1

Query: 2   SVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWD 61
           S+  S  EA E  VYSYTK+FNAFAAKLS  EAK +   ++V  V  N+YRKL TT+SWD
Sbjct: 62  SLNISQEEAKERKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWD 121

Query: 62  FIGLSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACN 121
           F+GL   A+R  K E D+I+G+ DTGITP ++SF D G GPPP KWKG+C  + NFT CN
Sbjct: 122 FVGLPLTAKRHLKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCN 181

Query: 122 KKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVP 181
            K+IGA+YFK DGN    ++ SP+D DGHGTHTSST  G  +A ASL G+A GTARG VP
Sbjct: 182 NKIIGAKYFKHDGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVP 241

Query: 182 SARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHA 241
           SAR+AMYKVCW  SGC+DMDILA F+AAI DGV++ISISIGG    +YS DSIS+G+FHA
Sbjct: 242 SARLAMYKVCWARSGCADMDILAGFEAAIHDGVEIISISIGGP-IADYSSDSISVGSFHA 301

Query: 242 MKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIF 301
           M+KGI+TV SAGN GP++G+V NH PWI+TVAAS IDR F S ++LGNGK+ SG+GI++F
Sbjct: 302 MRKGILTVASAGNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMF 361

Query: 302 NPKQKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIG 361
           +PK K YPLVSG D A+N++ K  A +C   +LD  KVKG ++ C++   G +S IKS G
Sbjct: 362 SPKAKSYPLVSGVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYG 421

Query: 362 ANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMV 421
             G II SD++LDNA IFMAPAT V+S VG+IIY YI STR+ +AVI KT+Q+   AP V
Sbjct: 422 GAGAIIVSDQYLDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFV 481

Query: 422 ASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACP 481
           ASFSSRGPNPGS R+LKPDIAAPG+DILAA+T  +SLTG  GDTQ+SKFT++SGTSMACP
Sbjct: 482 ASFSSRGPNPGSIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACP 541

Query: 482 HVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLI 541
           HVA  AAYVKSFHP W+PAAI+SA++T+A PISRR+N + EFAYG G +NP RA SPGL+
Sbjct: 542 HVAGVAAYVKSFHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLV 601

Query: 542 YDLNEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQP 601
           YD+++ISY+QFLC EGY  +++A L GT+S++CS+++PG GHDSLNYPT QL+L+S    
Sbjct: 602 YDMDDISYVQFLCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTS 661

Query: 602 MTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPS 661
               FRRRVTNVG P SVY AT+ AP GV ITV P +LSFS+  QKRSFKVVVKA  +  
Sbjct: 662 TLAVFRRRVTNVGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTP 721

Query: 662 AKMVSGSLAWVGAQHVVRSPIVVYSP 688
            K+VSG L W   +H VRSPIV+YSP
Sbjct: 722 GKIVSGLLVWKSPRHSVRSPIVIYSP 746

BLAST of Cucsa.019390 vs. TAIR10
Match: AT5G03620.1 (AT5G03620.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 640.2 bits (1650), Expect = 1.5e-183
Identity = 356/705 (50.50%), Postives = 461/705 (65.39%), Query Frame = 1

Query: 1   MSVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSW 60
           M+V    ++A E  +YSY K+ N F A+L   EA+ LS  + V  V  N  R+L TTRSW
Sbjct: 59  MTVIGDESKARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSW 118

Query: 61  DFIGL-SSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTA 120
           DF+GL  S  +RS   ES+IIVG+ DTGI   + SF D G GPPP KWKG C    NFT 
Sbjct: 119 DFLGLVESKYKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTR 178

Query: 121 CNKKLIGARYFKL--DGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTAR 180
           CN K+IGA+YF +  +G PD     +  D DGHGTHTSST  G +++ ASL G+A GTAR
Sbjct: 179 CNNKVIGAKYFHIQSEGLPDGEGDTA-ADHDGHGTHTSSTIAGVSVSSASLFGIANGTAR 238

Query: 181 GGVPSARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIG 240
           GGVPSAR+A YKVCW  SGC+DMD+LAAFD AI DGVD+ISISIGG     + +D I+IG
Sbjct: 239 GGVPSARIAAYKVCW-DSGCTDMDMLAAFDEAISDGVDIISISIGGASLP-FFEDPIAIG 298

Query: 241 AFHAMKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVG 300
           AFHAMK+GI+T  SAGN GP   +V N APW++TVAA+S+DRKF + ++LGNG   SG+ 
Sbjct: 299 AFHAMKRGILTTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGIS 358

Query: 301 INIFNPKQKMYPLVSGGDVARNSESK-DTASFCLEGTLDPTKVKGSLVFCKLLT------ 360
           +N FNP++KMYPL SG   +  S       S C  GTL   KV G +V+C+         
Sbjct: 359 LNGFNPRKKMYPLTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGGNG 418

Query: 361 -WGADSVIKSIGANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIY 420
             G D V++S+   GVI+Q  E  D A   +   + V    G  I  YI ST+ P AVI+
Sbjct: 419 GQGQDHVVRSLKGAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIF 478

Query: 421 KTKQLKAKAPMVASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSK 480
           KTK  K  AP ++SFS+RGP   S  ILKPDI+APG++ILAAY+ L S+TG   D + + 
Sbjct: 479 KTKTTKMLAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTL 538

Query: 481 FTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGN 540
           F++MSGTSMACPH AAAAAYVKSFHP WSPAAI+SAL+TTATP+  + N E E +YG+G 
Sbjct: 539 FSIMSGTSMACPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGN-EAELSYGSGQ 598

Query: 541 LNPSRAISPGLIYDLNEISYIQFLCSEGYTGSSIAVLSG-------TKSINCSNLIPGQG 600
           +NP RAI PGL+YD+ E +Y++FLC EGY  +SI +L+G        K  NC N+  G G
Sbjct: 599 INPRRAIHPGLVYDITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLG 658

Query: 601 HDSLNYPTFQLSLKSTNQPMTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFS 660
            D LNYP+    + ST   ++  F R VTNVG+  S Y A + AP G+ + V P  +SF 
Sbjct: 659 SDGLNYPSLHKQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFE 718

Query: 661 RLLQKRSFKVVVKASPLPSAK-MVSGSLAWVGAQ-HVVRSPIVVY 686
           R  +KR+FKVV+      + K +VS S+ W  ++ H+VRSPI+++
Sbjct: 719 RPKEKRNFKVVIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLF 759

BLAST of Cucsa.019390 vs. TAIR10
Match: AT5G59190.1 (AT5G59190.1 subtilase family protein)

HSP 1 Score: 599.7 bits (1545), Expect = 2.2e-171
Identity = 324/681 (47.58%), Postives = 442/681 (64.90%), Query Frame = 1

Query: 10  ASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIGLSSNA 69
           AS  +V SY +SFN FAA LS  E++ L   K+V  V P+K  +L TTRSWDF+G    A
Sbjct: 28  ASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKA 87

Query: 70  RRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACNKKLIGARY 129
           RR +  ESD+IVG+ D+GI P ++SF D+GFGPPPKKWKG+C     F ACN KLIGAR+
Sbjct: 88  RRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKF-ACNNKLIGARF 147

Query: 130 FKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSARVAMYK 189
           +    +       S  D +GHGTHT+STA GNA+  AS  GLA+GTARGGVPSAR+A YK
Sbjct: 148 YNKFAD-------SARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYK 207

Query: 190 VCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKKGIITV 249
           VC+    C+D+DILAAFD AI DGVDVISISI     +N  + S++IG+FHAM +GIIT 
Sbjct: 208 VCFNR--CNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITA 267

Query: 250 TSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPKQKMYP 309
            SAGN GP  GSV N +PW++TVAAS  DR+FI  + LGNGK ++G+ +N FN     +P
Sbjct: 268 GSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFP 327

Query: 310 LVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIGANGVIIQS 369
           +V G +V+RN  S+  A +C  G +D   VKG +V C       ++ +   GA GVI+Q+
Sbjct: 328 IVYGQNVSRNC-SQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYL--AGAIGVIVQN 387

Query: 370 DEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQL-KAKAPMVASFSSRG 429
               D+A +   PA+ +       I +YI+S   P A I +T+++   +AP V SFSSRG
Sbjct: 388 TLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRG 447

Query: 430 PNPGSHRILKPDIAAPGVDILAAYTPLKSLTG--QKGDTQYSKFTLMSGTSMACPHVAAA 489
           P+     +LKPD++APG++ILAA++P+ S +      D +  ++++MSGTSMACPHVA  
Sbjct: 448 PSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGV 507

Query: 490 AAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLIYDLNE 549
           AAYVKSFHP WSP+AI+SA++TTATP++ + NPE EFAYG+G +NP++A  PGL+Y++  
Sbjct: 508 AAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVET 567

Query: 550 ISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTTTF 609
             Y++ LC+EG+  +++   SG +++ CS     +    LNYPT    + S + P   TF
Sbjct: 568 EDYLKMLCAEGFDSTTLTTTSG-QNVTCSERTEVK---DLNYPTMTTFVSSLD-PFNVTF 627

Query: 610 RRRVTNVGHPISVYNAT-INAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSAKMV 669
           +R VTNVG P S Y A+ +   P + I++ P  L F  L +K+SF V +    L     V
Sbjct: 628 KRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFV 687

Query: 670 SGSLAWVGAQHVVRSPIVVYS 687
           S S+ W    H VRSPIV YS
Sbjct: 688 SSSVVWSDGSHSVRSPIVAYS 690

BLAST of Cucsa.019390 vs. TAIR10
Match: AT3G46850.1 (AT3G46850.1 Subtilase family protein)

HSP 1 Score: 573.5 bits (1477), Expect = 1.7e-163
Identity = 312/684 (45.61%), Postives = 435/684 (63.60%), Query Frame = 1

Query: 12  ESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIGLSSNAR- 71
           + +V +Y +SFN FAA+L++ E ++L++  +V  V P+K   LQTT SW+F+GL    R 
Sbjct: 68  DRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRT 127

Query: 72  -RSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACNKKLIGARY 131
            R+   ESD I+G+ D+GI P +DSF   GFGPPPKKWKG C    NFT CN KLIGARY
Sbjct: 128 KRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFT-CNNKLIGARY 187

Query: 132 F--KLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSARVAM 191
           +  KL+G P+     S  D  GHG+HT+S A GNA+   S  GL  GT RGGVP+AR+A+
Sbjct: 188 YTPKLEGFPE-----SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAV 247

Query: 192 YKVCWTSS-GCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKKGI 251
           YKVC      C+   ILAAFD AI D VD+I++S+G      + +D+++IGAFHAM KGI
Sbjct: 248 YKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGI 307

Query: 252 ITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPKQK 311
           +TV  AGN GP   ++V+ APW+ TVAAS+++R FI+ + LGNGK I G  +N F+   K
Sbjct: 308 LTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGK 367

Query: 312 MYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIGANGVI 371
            YPLV G   +   ++  +A FC  G LD  +VKG +V C   T       +++GA   I
Sbjct: 368 KYPLVYGKSASSRCDA-SSAGFCSPGCLDSKRVKGKIVLCD--TQRNPGEAQAMGAVASI 427

Query: 372 IQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQL-KAKAPMVASFS 431
           ++ + + D A +F  P +++S    NI+ +Y+ ST+ P A + K++ +   KAP+VAS+S
Sbjct: 428 VR-NPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYS 487

Query: 432 SRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACPHVAA 491
           SRGPNP  H ILKPDI APG +ILAAY+P   +   + DT++ K+T++SGTSM+CPHVA 
Sbjct: 488 SRGPNPLIHDILKPDITAPGSEILAAYSPY--VPPSESDTRHVKYTVISGTSMSCPHVAG 547

Query: 492 AAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPE---GEFAYGAGNLNPSRAISPGLIY 551
            AAY+K+FHPLWSP+ I+SA++TTA P++   +P     EFAYGAG+++P  AI PGL+Y
Sbjct: 548 VAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVY 607

Query: 552 DLNEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPM 611
           + N+  +I FLC   YTG  + ++SG  S +C+         +LNYP+    +  T +P 
Sbjct: 608 EANKSDHITFLCGFNYTGKKLRLISGDSS-SCTKEQTKSLTRNLNYPSMSAQVSGT-KPF 667

Query: 612 TTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSA 671
             TFRR VTNVG P + Y A +     + + V P  LS   L +K+SF V V  +   + 
Sbjct: 668 KVTFRRTVTNVGRPNATYKAKV-VGSKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAE 727

Query: 672 KMVSGSLAWVGAQHVVRSPIVVYS 687
            +VS  L W    H VRSPIVVY+
Sbjct: 728 NLVSAQLIWSDGVHFVRSPIVVYA 736

BLAST of Cucsa.019390 vs. TAIR10
Match: AT5G59090.1 (AT5G59090.1 subtilase 4.12)

HSP 1 Score: 553.5 bits (1425), Expect = 1.8e-157
Identity = 314/685 (45.84%), Postives = 432/685 (63.07%), Query Frame = 1

Query: 14  MVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIGLSS--NARR 73
           +V SY +SFN FAA+L++ E  L++  + V  V PNK  +L TT SWDF+G+    N +R
Sbjct: 69  LVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKR 128

Query: 74  STKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACNKKLIGARYFK 133
           +   ESD I+G+ DTGI P + SF D GFGPPPKKWKG C    NFT CN KLIGAR + 
Sbjct: 129 NLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFT-CNNKLIGARDYT 188

Query: 134 LDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSARVAMYKVC 193
            +G  D         T GHGTHT+STA GNA+   S  G+  GT RGGVP++R+A YKVC
Sbjct: 189 SEGTRD---------TSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVC 248

Query: 194 WTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKKGIITVTS 253
            T SGCS   +L++FD AI DGVD+I+ISIG    + + DD I+IGAFHAM KGI+TV+S
Sbjct: 249 -TDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSS 308

Query: 254 AGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPKQKMYPLV 313
           AGN GP   +V + APWI TVAAS+ +R FI+ + LGNGK ++G  +N F+ K K YPLV
Sbjct: 309 AGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLV 368

Query: 314 SGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADS---VIKSIGANGVIIQ 373
            G   A ++    TA+ C    L+ ++VKG ++ C     G  S   + KS+GA  +I +
Sbjct: 369 YGKSAASSACDAKTAALCAPACLNKSRVKGKILVC-----GGPSGYKIAKSVGAIAIIDK 428

Query: 374 SDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQL-KAKAPMVASFSSR 433
           S    D A     PA+ + +     + +YI+S  +P A + KT+ +    +P++ASFSSR
Sbjct: 429 SPR-PDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSR 488

Query: 434 GPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACPHVAAAA 493
           GPN  +  ILKPDI APGV+ILAA++P  +    + DT+  K+++ SGTSMACPHVA  A
Sbjct: 489 GPNTIAVDILKPDITAPGVEILAAFSP--NGEPSEDDTRRVKYSVFSGTSMACPHVAGVA 548

Query: 494 AYVKSFHPLWSPAAIRSALLTTATPISR--RLNPEGEFAYGAGNLNPSRAISPGLIYDLN 553
           AYVK+F+P WSP+ I+SA++TTA P+    R     EFAYGAG+++P  A++PGL+Y+L+
Sbjct: 549 AYVKTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELD 608

Query: 554 EISYIQFLCSEGYTGSSIAVLSGTKSINCS---NLIPGQGHDSLNYPTFQLSLKSTNQPM 613
           +  +I FLC   YT  ++ ++SG  ++ CS    ++P     +LNYP+    L  T+   
Sbjct: 609 KADHIAFLCGMNYTSKTLKIISG-DTVKCSKKNKILP----RNLNYPSMSAKLSGTDSTF 668

Query: 614 TTTFRRRVTNVGHPISVYNATINAPPG--VTITVTPPTLSFSRLLQKRSFKVVVKASPLP 673
           + TF R +TNVG P S Y + + A  G  ++I VTP  L F  + +K+SF V V  S + 
Sbjct: 669 SVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVD 728

Query: 674 SAKMVSGSLAWVGAQHVVRSPIVVY 686
           S    S +L W    H VRSPIVVY
Sbjct: 729 SEVPSSANLIWSDGTHNVRSPIVVY 729

BLAST of Cucsa.019390 vs. NCBI nr
Match: gi|778697899|ref|XP_011654432.1| (PREDICTED: subtilisin-like protease SBT4.14 [Cucumis sativus])

HSP 1 Score: 1372.8 bits (3552), Expect = 0.0e+00
Identity = 687/687 (100.00%), Postives = 687/687 (100.00%), Query Frame = 1

Query: 1   MSVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSW 60
           MSVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSW
Sbjct: 57  MSVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSW 116

Query: 61  DFIGLSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTAC 120
           DFIGLSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTAC
Sbjct: 117 DFIGLSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTAC 176

Query: 121 NKKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGV 180
           NKKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGV
Sbjct: 177 NKKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGV 236

Query: 181 PSARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFH 240
           PSARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFH
Sbjct: 237 PSARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFH 296

Query: 241 AMKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINI 300
           AMKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINI
Sbjct: 297 AMKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINI 356

Query: 301 FNPKQKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSI 360
           FNPKQKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSI
Sbjct: 357 FNPKQKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSI 416

Query: 361 GANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPM 420
           GANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPM
Sbjct: 417 GANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPM 476

Query: 421 VASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMAC 480
           VASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMAC
Sbjct: 477 VASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMAC 536

Query: 481 PHVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGL 540
           PHVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGL
Sbjct: 537 PHVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGL 596

Query: 541 IYDLNEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQ 600
           IYDLNEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQ
Sbjct: 597 IYDLNEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQ 656

Query: 601 PMTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLP 660
           PMTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLP
Sbjct: 657 PMTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLP 716

Query: 661 SAKMVSGSLAWVGAQHVVRSPIVVYSP 688
           SAKMVSGSLAWVGAQHVVRSPIVVYSP
Sbjct: 717 SAKMVSGSLAWVGAQHVVRSPIVVYSP 743

BLAST of Cucsa.019390 vs. NCBI nr
Match: gi|659109517|ref|XP_008454753.1| (PREDICTED: xylem serine proteinase 1-like [Cucumis melo])

HSP 1 Score: 1308.1 bits (3384), Expect = 0.0e+00
Identity = 651/687 (94.76%), Postives = 667/687 (97.09%), Query Frame = 1

Query: 1   MSVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSW 60
           MSVKKSHAEASESMVYSYTKSFNAFAAKLSD EAKLLSTRKDVHHVIPN YRKLQTTRSW
Sbjct: 60  MSVKKSHAEASESMVYSYTKSFNAFAAKLSDHEAKLLSTRKDVHHVIPNTYRKLQTTRSW 119

Query: 61  DFIGLSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTAC 120
           DFIGLSSNARR TKHESDIIVGLFDTGITPTADSF+DDGFGPPPKKWKGTCHHFANFTAC
Sbjct: 120 DFIGLSSNARRRTKHESDIIVGLFDTGITPTADSFRDDGFGPPPKKWKGTCHHFANFTAC 179

Query: 121 NKKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGV 180
           NKKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNA+AGASLSGLAEGTARGGV
Sbjct: 180 NKKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGV 239

Query: 181 PSARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFH 240
           PSARVAMYKVCW SSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFH
Sbjct: 240 PSARVAMYKVCWASSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFH 299

Query: 241 AMKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINI 300
           AMKKGIITVTSAGNGGP AGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINI
Sbjct: 300 AMKKGIITVTSAGNGGPMAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINI 359

Query: 301 FNPKQKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSI 360
           FNPK+KMYPLVSGGDVARNSESKDTASFCLE +LDPTKVKGSLVFCKLLTWGADSVIKS+
Sbjct: 360 FNPKKKMYPLVSGGDVARNSESKDTASFCLEDSLDPTKVKGSLVFCKLLTWGADSVIKSV 419

Query: 361 GANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPM 420
           GANGVIIQSD+FLDNADIFMAPATMVSS VG IIYTYIKSTRTPTAVIYKT+QLKAKAPM
Sbjct: 420 GANGVIIQSDQFLDNADIFMAPATMVSSSVGTIIYTYIKSTRTPTAVIYKTRQLKAKAPM 479

Query: 421 VASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMAC 480
           VASFSSRGPNPGSHRILKPDIAAPGV+ILA YTPLKSLTGQKGDTQYSKFT+MSGTSMAC
Sbjct: 480 VASFSSRGPNPGSHRILKPDIAAPGVNILAGYTPLKSLTGQKGDTQYSKFTIMSGTSMAC 539

Query: 481 PHVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGL 540
           PHVAAAAAYVKSFHPLWSPAAIRSALLTT  PISRRLNPEGEFAYGAGNLNPS+AISPGL
Sbjct: 540 PHVAAAAAYVKSFHPLWSPAAIRSALLTTTRPISRRLNPEGEFAYGAGNLNPSKAISPGL 599

Query: 541 IYDLNEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQ 600
           IYDLNE+SYIQFLCSEGYTGSSIAVL+GTKSINCS LIPG GHDSLNYPTFQLSL+ST Q
Sbjct: 600 IYDLNEMSYIQFLCSEGYTGSSIAVLAGTKSINCSTLIPGHGHDSLNYPTFQLSLRSTKQ 659

Query: 601 PMTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLP 660
           PMTTTFRR+VTNVG P+SVYNATI APPGV ITVTP TLSFSRLLQKRSFKVVVKASPL 
Sbjct: 660 PMTTTFRRQVTNVGQPVSVYNATIKAPPGVKITVTPTTLSFSRLLQKRSFKVVVKASPLQ 719

Query: 661 SAKMVSGSLAWVGAQHVVRSPIVVYSP 688
           SAKMVSGSLAWVG +HVVRSPIVVYSP
Sbjct: 720 SAKMVSGSLAWVGDRHVVRSPIVVYSP 746

BLAST of Cucsa.019390 vs. NCBI nr
Match: gi|659109757|ref|XP_008454861.1| (PREDICTED: xylem serine proteinase 1-like [Cucumis melo])

HSP 1 Score: 1109.7 bits (2869), Expect = 0.0e+00
Identity = 545/687 (79.33%), Postives = 608/687 (88.50%), Query Frame = 1

Query: 1   MSVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSW 60
           MSVK+SH EA ESMVYSYT +FNAFAAKL++ EAK LS R DV HVIPN+YRKLQTTRSW
Sbjct: 89  MSVKESHVEAKESMVYSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSW 148

Query: 61  DFIGLSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTAC 120
           DF+G   NA+R T+ ESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTC HFANF+ C
Sbjct: 149 DFLGFPINAKRKTRQESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCDHFANFSGC 208

Query: 121 NKKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGV 180
           N KLIGARYFKLDG  +P DILSP+D +GHGTHTSSTATGN I GA+LSGLA+GTARGGV
Sbjct: 209 NNKLIGARYFKLDGLTEPLDILSPIDINGHGTHTSSTATGNVITGANLSGLAQGTARGGV 268

Query: 181 PSARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFH 240
           PSARVAMYKVCWTS+GCSDMD+LAAFDAAIQDGVDVISISI G G+ NY++D ISIGAFH
Sbjct: 269 PSARVAMYKVCWTSTGCSDMDLLAAFDAAIQDGVDVISISIAGIGYGNYTEDPISIGAFH 328

Query: 241 AMKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINI 300
           AMKKGIITVT+AGN GP+AG+VVNHAPWI+TVAASSIDR FISP+ELGNGKNISGVGIN+
Sbjct: 329 AMKKGIITVTAAGNNGPSAGTVVNHAPWILTVAASSIDRTFISPVELGNGKNISGVGINL 388

Query: 301 FNPKQKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSI 360
           FNP++KMY LVSG DVA+N ESKD A++C+E +LDP+KVK S+VFCKLLTWGADS +KSI
Sbjct: 389 FNPEKKMYKLVSGEDVAKNIESKDDATYCVENSLDPSKVKDSIVFCKLLTWGADSAVKSI 448

Query: 361 GANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPM 420
           GA G I+QSD+FLDN DIFMAP+T+VSS VG  I +YI S+RTPTAVIYKT+Q    AP+
Sbjct: 449 GAAGAILQSDQFLDNTDIFMAPSTLVSSFVGATIDSYIHSSRTPTAVIYKTRQHIGAAPI 508

Query: 421 VASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMAC 480
           VA FSSRGPNPGS RILKPDIAAPGV+ILA YTPLKSLTG KGDTQ+SKFTLMSGTSMAC
Sbjct: 509 VAPFSSRGPNPGSTRILKPDIAAPGVNILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMAC 568

Query: 481 PHVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGL 540
           PHVAAAAAYVKSFHPLWSPAAIRSALLTTA PISRR NP+GEF YGAGNLNP +A +PGL
Sbjct: 569 PHVAAAAAYVKSFHPLWSPAAIRSALLTTAKPISRRGNPDGEFGYGAGNLNPGKAKNPGL 628

Query: 541 IYDLNEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQ 600
           IYDLNE+SYIQFLCSEGY+GSSI++L+GTKSINC+ +I GQG+DSLNYPTFQL L+ST Q
Sbjct: 629 IYDLNEMSYIQFLCSEGYSGSSISILTGTKSINCATIIRGQGYDSLNYPTFQLRLQSTRQ 688

Query: 601 PMTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLP 660
           P T  F R VTNVG P+SVYN T+ APPGV ITV P TLSFS LLQK  FKVVVKA+PLP
Sbjct: 689 PTTAVFWREVTNVGTPVSVYNTTVWAPPGVEITVEPATLSFSYLLQKERFKVVVKANPLP 748

Query: 661 SAKMVSGSLAWVGAQHVVRSPIVVYSP 688
           S KMVSGS+ W   ++VVRSPIVVYSP
Sbjct: 749 SNKMVSGSITWFNPRYVVRSPIVVYSP 775

BLAST of Cucsa.019390 vs. NCBI nr
Match: gi|778730647|ref|XP_004140478.2| (PREDICTED: subtilisin-like protease SBT4.14 [Cucumis sativus])

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 532/687 (77.44%), Postives = 602/687 (87.63%), Query Frame = 1

Query: 1   MSVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSW 60
           MSVK+SH +A + MV+SYT +FNAFAAKL++ EAK LS R DV HVIPN+YRKLQTTRSW
Sbjct: 22  MSVKESHVDAKDCMVHSYTNNFNAFAAKLTEAEAKTLSERGDVQHVIPNRYRKLQTTRSW 81

Query: 61  DFIGLSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTAC 120
           DF+G   NA+R T+ ESDIIVGLFDTGITPTADSFKDDG+GPPPKKWKGTC HFANF+ C
Sbjct: 82  DFLGFPINAKRKTRQESDIIVGLFDTGITPTADSFKDDGYGPPPKKWKGTCDHFANFSGC 141

Query: 121 NKKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGV 180
           N KLIGARYFKLDG  +P DILSPVD +GHGTHTSSTATGN I GA+LSGLA+GTA GGV
Sbjct: 142 NNKLIGARYFKLDGITEPFDILSPVDVNGHGTHTSSTATGNVITGANLSGLAQGTAPGGV 201

Query: 181 PSARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFH 240
           PSAR+AMYKVCW S+GCSDMD+LAAFDAAIQDGVDVISISI G G+ NY+DD ISIGAFH
Sbjct: 202 PSARLAMYKVCWMSNGCSDMDLLAAFDAAIQDGVDVISISIAGIGYGNYTDDPISIGAFH 261

Query: 241 AMKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINI 300
           AMKKGIITVT+AGN GP+AG+VVNHAPWI+TVAASSIDR+FISP+ELGNGKNISGVGIN+
Sbjct: 262 AMKKGIITVTAAGNNGPSAGTVVNHAPWILTVAASSIDRRFISPVELGNGKNISGVGINL 321

Query: 301 FNPKQKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSI 360
           FNP++KMY LVSG DVA+N E KD A +C + +LDP+KVK SLVFCKL+TWGADS +KSI
Sbjct: 322 FNPEKKMYKLVSGEDVAKNIEGKDNAMYCEDKSLDPSKVKDSLVFCKLMTWGADSTVKSI 381

Query: 361 GANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPM 420
           GA G I+QSD+FLDN DIFMAP+ +VSS VG  I  YI STRTPTAVIYKT+Q +A AP+
Sbjct: 382 GAAGAILQSDQFLDNTDIFMAPSALVSSFVGATIDAYIHSTRTPTAVIYKTRQHRAAAPI 441

Query: 421 VASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMAC 480
           +A FSSRGPNPGS  ILKPDIAAPGV+ILA YTPLKSLTG KGDTQ+SKFTLMSGTSMAC
Sbjct: 442 IAPFSSRGPNPGSTHILKPDIAAPGVNILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMAC 501

Query: 481 PHVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGL 540
           PHVAAAAAYVKSFHPLWSPAAIRSALLTTA PISRR NP+GEF YGAGNLNP +A +PGL
Sbjct: 502 PHVAAAAAYVKSFHPLWSPAAIRSALLTTAKPISRRGNPDGEFGYGAGNLNPRKAKNPGL 561

Query: 541 IYDLNEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQ 600
           IYDLNE+SYIQFLC EGY+GSSI +L+GTKSINC+ +IPG+G+DSLNYPTFQLSL+S+ +
Sbjct: 562 IYDLNEMSYIQFLCREGYSGSSIIILTGTKSINCATIIPGEGYDSLNYPTFQLSLQSSRE 621

Query: 601 PMTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLP 660
           P T  F R VTNVG P+SVYNAT+ APPGV ITV P TLSFS L QK  FKVVVKA+PLP
Sbjct: 622 PTTAVFWREVTNVGKPVSVYNATVRAPPGVEITVEPATLSFSYLHQKERFKVVVKANPLP 681

Query: 661 SAKMVSGSLAWVGAQHVVRSPIVVYSP 688
           + KMVSGS+ W   ++VVRSP+VVYSP
Sbjct: 682 ANKMVSGSITWFDPRYVVRSPVVVYSP 708

BLAST of Cucsa.019390 vs. NCBI nr
Match: gi|645254181|ref|XP_008232921.1| (PREDICTED: xylem serine proteinase 1 [Prunus mume])

HSP 1 Score: 984.2 bits (2543), Expect = 1.2e-283
Identity = 481/687 (70.01%), Postives = 566/687 (82.39%), Query Frame = 1

Query: 2   SVKKSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWD 61
           SVK+S+ EA ESMVYSYTKSFNAFAAKLS DEA+ LS   +V  V+PN+Y KL TT+SWD
Sbjct: 66  SVKESYVEAKESMVYSYTKSFNAFAAKLSSDEAERLSGMDEVLSVLPNRYHKLHTTKSWD 125

Query: 62  FIGLSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACN 121
           FIGL   ARR+ K E DIIVGL DTGITP + SFK DGFGPPP KWKGTC HFANF+ CN
Sbjct: 126 FIGLPLKARRNLKLERDIIVGLLDTGITPESKSFKGDGFGPPPAKWKGTCGHFANFSGCN 185

Query: 122 KKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVP 181
            K+IGA+YFKLDGNPDP+DILSP+D DGHGTHTSST  GN +  ASL GLA GTARG VP
Sbjct: 186 NKIIGAKYFKLDGNPDPADILSPIDVDGHGTHTSSTLAGNQVPNASLFGLAMGTARGAVP 245

Query: 182 SARVAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHA 241
           SARVAMYKVCW SSGC+DMDILAA+DAAI DGVDVISISIGGGG  NY  DSI+IGAFHA
Sbjct: 246 SARVAMYKVCWASSGCTDMDILAAYDAAIHDGVDVISISIGGGG-PNYVRDSIAIGAFHA 305

Query: 242 MKKGIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIF 301
           MKKGIITV SAGN GP+ GSVVNHAPW VTVAAS IDR+FIS + LGNGK++SG+G+N F
Sbjct: 306 MKKGIITVASAGNDGPSLGSVVNHAPWFVTVAASGIDRQFISKVHLGNGKDVSGIGVNTF 365

Query: 302 NPKQKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIG 361
           NPK+ +YPLVSG DVARNS++K++A FCL+ +LDP KVKG LV CKL  WGADSV+K IG
Sbjct: 366 NPKKNLYPLVSGVDVARNSQTKESARFCLQDSLDPNKVKGKLVLCKLENWGADSVVKGIG 425

Query: 362 ANGVIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMV 421
             G II+ D+ LD A IFMAP TMV+  +G II  YI ST +P+AVI K+ + K +AP +
Sbjct: 426 GVGTIIEDDQLLDAAQIFMAPGTMVNDTIGEIIDEYIHSTGSPSAVISKSHEAKKRAPFI 485

Query: 422 ASFSSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACP 481
           ASFSSRGPNPGS RILKPDIAAPGVDILA+YT  KSLTG +GDTQYS+FT+MSGTSM+CP
Sbjct: 486 ASFSSRGPNPGSERILKPDIAAPGVDILASYTLRKSLTGLEGDTQYSEFTIMSGTSMSCP 545

Query: 482 HVAAAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLI 541
           HVA   AYVKSFHP WSPAAIRSA+LTTA P+S+R+N + EF+YG G +NP++A++PGL+
Sbjct: 546 HVAGVVAYVKSFHPHWSPAAIRSAILTTAKPMSQRVNSDAEFSYGVGQVNPTKAVNPGLV 605

Query: 542 YDLN-EISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQ 601
           YD++  +SYIQFLC EGY GSS+A L G+KS+NCS+L+PGQG+D+LNYPT QLSLKS+ Q
Sbjct: 606 YDMDGTLSYIQFLCHEGYKGSSLAPLVGSKSVNCSSLLPGQGYDALNYPTMQLSLKSSKQ 665

Query: 602 PMTTTFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLP 661
           P    FRR VTNVG   S YNATI AP GV I V P +LSFS  L+KR+F+VVVKA+P+P
Sbjct: 666 PTVGVFRRTVTNVGPAKSTYNATIRAPKGVEIIVKPMSLSFSTTLEKRTFQVVVKANPMP 725

Query: 662 SAKMVSGSLAWVGAQHVVRSPIVVYSP 688
           S +M+SGSL W  +++ VRSPIV++SP
Sbjct: 726 SMRMISGSLGWKSSRYTVRSPIVIHSP 751

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SBT4E_ARATH1.4e-25562.68Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana GN=SBT4.14 PE=2 SV=1[more]
SBT4F_ARATH2.6e-18250.50Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana GN=SBT4.15 PE=3 SV=1[more]
CUCM1_CUCME2.3e-17046.40Cucumisin OS=Cucumis melo PE=1 SV=1[more]
SBT43_ARATH3.9e-17047.58Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1[more]
SBT46_ARATH3.0e-16245.61Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana GN=SBT4.6 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KME6_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G000010 PE=4 SV=1[more]
M5X7G0_PRUPE3.8e-28169.29Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021240mg PE=4 SV=1[more]
A0A067HDF0_CITSI5.5e-28068.91Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g004503mg PE=4 SV=1[more]
B9RDI2_RICCO2.6e-27768.95Xylem serine proteinase 1, putative OS=Ricinus communis GN=RCOM_1613010 PE=4 SV=... [more]
E6NU20_JATCU8.2e-27668.51JHL05D22.1 protein OS=Jatropha curcas GN=JHL05D22.1 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G00230.17.6e-25762.68 xylem serine peptidase 1[more]
AT5G03620.11.5e-18350.50 Subtilisin-like serine endopeptidase family protein[more]
AT5G59190.12.2e-17147.58 subtilase family protein[more]
AT3G46850.11.7e-16345.61 Subtilase family protein[more]
AT5G59090.11.8e-15745.84 subtilase 4.12[more]
Match NameE-valueIdentityDescription
gi|778697899|ref|XP_011654432.1|0.0e+00100.00PREDICTED: subtilisin-like protease SBT4.14 [Cucumis sativus][more]
gi|659109517|ref|XP_008454753.1|0.0e+0094.76PREDICTED: xylem serine proteinase 1-like [Cucumis melo][more]
gi|659109757|ref|XP_008454861.1|0.0e+0079.33PREDICTED: xylem serine proteinase 1-like [Cucumis melo][more]
gi|778730647|ref|XP_004140478.2|0.0e+0077.44PREDICTED: subtilisin-like protease SBT4.14 [Cucumis sativus][more]
gi|645254181|ref|XP_008232921.1|1.2e-28370.01PREDICTED: xylem serine proteinase 1 [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000209Peptidase_S8/S53_dom
IPR010259S8pro/Inhibitor_I9
IPR015500Peptidase_S8_subtilisin-rel
IPR023828Peptidase_S8_Ser-AS
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006508 proteolysis
cellular_component GO:0009505 plant-type cell wall
molecular_function GO:0032440 2-alkenal reductase [NAD(P)] activity
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.019390.1Cucsa.019390.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 418..539
score: 1.5E-76coord: 29..277
score: 1.5
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 77..528
score: 3.1
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 419..536
score: 6.68E-71coord: 61..289
score: 6.68
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 5..54
score: 1.5
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 474..490
score: 1.9E-10coord: 76..95
score: 1.9E-10coord: 146..159
score: 1.9
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 2..687
score: 1.8E
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 475..485
scor
NoneNo IPR availablePANTHERPTHR10795:SF320SUBTILASE FAMILY PROTEIN-RELATEDcoord: 2..687
score: 1.8E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.019390Csa5G000010Cucumber (Chinese Long) v2cgycuB394
Cucsa.019390CSPI05G00020Wild cucumber (PI 183967)cgycpiB417
Cucsa.019390CsaV3_5G013700Cucumber (Chinese Long) v3cgycucB424
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None