CSPI05G00150.2 (mRNA) Wild cucumber (PI 183967)

NameCSPI05G00150.2
TypemRNA
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionSerine--tRNA ligase
LocationChr5 : 191826 .. 196636 (-)
Sequence length1347
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGTTTTCTCACTCTTAATTGCAGTTTTTGGAAGGGAAATCTCAGGCGCAGATAGACAAAGATGTTGGACATCAATTTGTTCAGAGAAGAGAAAGGCAACAATCCTGAAATCATCCGTGAGTCTCAACGCCGCCGATTTGCCAACGTTGAACTCGTTGATGAAGTCATTAAGCTTGATAAAGAATGGCGTCAACGTAATCCTCCATCTTTTATCCTCTACTATTTCATTTCTATCGCTTTTTCTTACTTTGTTTTCTTTTTCTTTTTTTGGTATTGGAATTGGTATTTGTTTAGGCCAATTTGAGCTGGAGAATCTCAGAAAGGAGTTCAACAAGATCAACAAGCAAATTGCTCAGCTTAGAATAGTAATTCATCTATCGATCTTTCTTCTTTCACGTTGCAGGAAATTGTCTTAGGAGCTAATGTTAGTTTCATAGTTCATGCAACTGTTAGTCCCTATTACGGGATTTAAGTTGTATGCTGTTAGACCTCCTATAAACTATACGTATAAACTGGATAGTGGAATGTTAATATTTGTTTGTACGGATAGTGGGGTGCCGGACCAGAGGAGGTTTAAGAAAGGTTCCTGAGTGCAGGAACTGTACTTAGATATCTAACATGTCTTGTGAAGGTTTTGGTTTTGGTTCAGTTTCCATTGTTCCGTTGTTTACTTGGTAAATTTTGGAAATGAAGGCTGGTGAGGATGCGTCCGAGAGGATTAAAGACACTGAAGAAAATAAAAAGTTGTCTGCTGAGAAAGATGTTGAAGCTAAGGATGTGTTAAAGAAGTTGAACTCAAAACTGGAAATAATTGGAAACCTTGTGCATGATTCCGTTCCCGTCAGCAATGATGAGGTATGATATTTGGCATCACTGCATTTGTTTTATTCTTTGTAGTGCTTTACTTTCTTAACAATGCAAGCAACTTTTTGTAGGCTAATAATGTTGTTATCAGATCGTGGGGTGAGAAACTAGTAAAACCGAAACTGAAGAATCATGTTGATCTTGTTGAGCTTCTTGAAATTGCAGATTCTAAAAGAGGTAATAAAGTATAGAAATTTTACTCTGATCAGTTGAGATATGCACAAATTTCTTATCATAGCTTCTTAATAACCTCCCAAATTTTCCCACTAAGCTTGTCACTGCTATCATAGGTAAACTCACAATCCAATCCTGAATTCGTCTTCACATTCCTCTGCTTTAGGGTTTAGGGTTTAGGGTAATCAACGGACTCTTTGGTAAAACCCATTTCCTACCTGATAAGTTAGATACAGAGAAAGAAAAAAGTCTAGAAATTTCCTTCTGTTCCGGTATAAACATTTGTGACCTTTTCCCCTTAAAGGATTTGGAAAAACTATAGCGCAGCGACATCCGTGTCCATTGGCACTCTATTGGAGTGAAAAAGAACTTCCTATTCACGATATTTCAGTGGGTAACTCATCACTGCAACCAAACTATCTCTTACGCACGGTGAAGTACCTAGCTTCTTGTTCTAACTTAACAACCTCTGGACCTGTGTTCCTATATGACTACTCTTCTTCCATTTCCACTATTTTGGAGTGAAAAAAACTTTCTATTCACTGTAACCTTTCCCATCATATTCTCATTTTAGAGAGGAGAGAAAAACTAAGCAACATGATTGTTAAGCAATCAGCTTCGTACATGATAAGAGGACATTATGGTTGTTGATTATTAGAGCTTTCGGAGATTAGTGAATCACAAGGAGCCACCAAACTTGCAATGACATTCTCACTTCTAATAGTTTATATACCTTTAGCTCCTGATGTTTTCTCTGGTATATATCGTCTCATCAAATTAAACTCCCAAATTTCACATTTTTCACTGCTATGGAATCCATCCAGTTTATGCCTTCTATATTCCCATTGAAAATTTAAAAGATACAGAATATTATGCTAATTTCAACTTTATGGAAATATTTAGGTTCTGAAATAGCAGGAGGTAGAGGCTACTATCTAAAAGGAGCTGGTGTGCGTTTGAATCAAGCTTTGATAAACTATGGCCTTGACTTTTTGGAGAAAAGGGGTTATACAGAACTGCAGACTCCTTTCTTCATGAGAAAAGATATCATGGCTAGATGTGCTCAATTGGCACAATTTGACGAAGAACTTTATAAGGTCTGTTATTAATGTTTTGACAGGATCTATTGGAATTTAAATCCCAATGGTTCTGAAATGAATTCATTCCTTCTTTAAATAAAAGGATATTGATTTCAAATATTGTTGGATGTGTGTGTGTATGTGTGTATGTGTGTATAGGATATAACAGGATTTCAAATTCAAATACTAATTACGATTTTTTCCTTTTCATTTCTAGTAATCATTTGAACAATTTTATCCAAATTTATTTCAAATTCTTCTCATTCCAAACAAAACAAATTAAAGATAATTCATAAAGTTTCAAATCATGTGATAATCCCTTTTTATACTTCTTTGATCCCAAACAAATCTTGATGACTTTCTTATGTGGAAAGTAAATTTTTATTTGTAGTCTTGCATTTCAAAATAAGGATCTTGGTCCATTAGTGAACCGTTTTTTTTTGTTGTGTTTGGGCTGTGGAAGATTGCCAAAATATGATCATGAATTCATTGGTGTTCATTAGGTTACTGGTGAGGGTGATGATAAATATCTAATTGCCACCGCTGAACAACCACTTTGTTCATATCATCTTGAAGATTGGATTCATCCTTCTCAATTGCCTATAAGGTTTCTACATCTTACTAAAAATTGTCCACCTCTAGAACCCTATGAAAGCATTTTATTTTAATTCACTATTATTAATGCACCAAATAGGTACGCTGGATATTCATCTTGCTTTCGTAAAGAAGCAGGTTCCCATGGCCGAGATACTCTTGGAATCTTCCGAGTCCACCAATTCGAGAAAGTAGAGCAATTTTGTATCACCAGCCCTAACGACAATGATTCGTGGGACATGCATGAAGAGATGATAAAAAATTCCGAGGAATTCTACAAGACGGTAGCTTCTATACCTTGTATTGATGATTTTTCTGTTCAACAATTATAATTGCAAATCAGTAATCTGATATTTAGGCAATTTTATGAAACATTTGGCTAGAGAGGAAGTGTTATTTCTATACCCTGCAAGTGTTATTTTTTTATCTTTAGGATTTTTCTTTTTAATACTTTAAAAAATATTATCTATGAGCTCCTGTCCTTTGGATTGGAGCCTTTTTGGTTTTGGTTTTGGTTTTGGTTTTGATTTGGTGCTTATCTATTTGCTCTTGGAATCTATATTTCTTTTGAACTTTGGGCTTTAGGATCTCTCATCTTGTATCCCCTTTTGTTTTATTGTATTCCATCGTTTTCTTCTCTGTGGAAGCTTGGTTTTCCATTAACAAAACAATATTAGTGGTAGTAATTTTGTCTGATGTGAGATCTTATGTTTTGGCAGTTAAATATCCCATACCAAGTTGTGGCTATAGTTTCTGGTGCATTAAATGATGCAGCTGCCAAGAAATATGACTTAGAAGCATGGTTTCCTGCATCTCAGACTTACAGAGAGTTAGTATCATGTTCTAATTGCACTGACTATCAGTCCAGAAGGTTAGAGATTCGATACGGTCAGAAAAAGGTAAGTTCTTCATTACTTCTGAATTATTGAAGAATATGATTTGCATTGTGATTGTTGAACTTGGACGTGCTAACAGCTAACAGGCTTCTAATAGGGTAATCATTGATTTTTATAAATTAATTTATGTCTATTATCCTGGCATTCTTGTAACTTCTCAATGAAATTGGGTGGGTTAAATTATAAGCTTGGTCCTGAATTTTCACGGTTATATGTTAATTTATTGTATTTTCTGCCTCTAAGAATTTAATGCTTAGACCACACCGTATCTAAACCTGATTTAAGTCTGGTTATTTTTGAAAGCTGTCTATTTCATTGAAAAAAGTTTGGTTCTCATGCCACAAAATATCTAAACCACTCTGAATTCAACTCTGCATTAAACGCTTGACACTGAGCAGTTGAGACATACCGTCAAAGTGGTTAAGTACTTAATTGATACTGTTAACAACGTAGACCTATTAGACACAACATTCAAATTGATTGGTTGAGGGAATAATTAAAAAAAATTTCAAGTACTAGGATTAGATGGACAAACTTGCAAGTTTGAAGGGTTTATGATAAACTTTGTAAAGTATGCAACCACTAAATAGACACAACCTTGCAAGTTAGTTGGGGGGTTATTATAAACTTTTGAAGCACACCACATTATTACTTTCCTTTTTAATTTCATAGTGTTCAACTGCTATTTTGTTTTGATGGTGCAACTGCAGAGCAACGAGCAGACGAAACAGTATGTGCATTTGCTAAATTCTACACTCACCGCAACAGAAAGGACTCTATGCTGCATTCTTGAGAACTACCAAAAAGAAGATGGGGTTGAAGTACCTGAAGTTCTACGACCATATTTGGGTGGAAAGGCATTTCTACCCTTCAAAACAAAACCGACAGGGAGCGATTCCAAAGGGAAGAAATCGAAGGCCTAATTGTGTACTTTTCTTTTCTCTCCCTTTGAATTATTAAATCTTCTGGCCAAGGAAAAAGGAAATTGCTCAGAATATTGTGTTAGAACTTCAAATTATATTTTTGGTGGGTATTGAATTATGTTAAACACTAACACTACTAGCCACCATGGCTGTTACGGTTGATTCATCTTTTGAAAACCTACTTTTGTTTTATCGTCAGTATACACTCTAGAAAACTGTTATAAATTGAACTTTCATATATCTGGGTTTGTTTTCTTTTTTTCATTCAGAAAAGTTTGAC

mRNA sequence

ATGTTGGACATCAATTTGTTCAGAGAAGAGAAAGGCAACAATCCTGAAATCATCCGTGAGTCTCAACGCCGCCGATTTGCCAACGTTGAACTCGTTGATGAAGTCATTAAGCTTGATAAAGAATGGCGTCAACGCCAATTTGAGCTGGAGAATCTCAGAAAGGAGTTCAACAAGATCAACAAGCAAATTGCTCAGCTTAGAATAGCTGGTGAGGATGCGTCCGAGAGGATTAAAGACACTGAAGAAAATAAAAAGTTGTCTGCTGAGAAAGATGTTGAAGCTAAGGATGTGTTAAAGAAGTTGAACTCAAAACTGGAAATAATTGGAAACCTTGTGCATGATTCCGTTCCCGTCAGCAATGATGAGGCTAATAATGTTGTTATCAGATCGTGGGGTGAGAAACTAGTAAAACCGAAACTGAAGAATCATGTTGATCTTGTTGAGCTTCTTGAAATTGCAGATTCTAAAAGAGGTTCTGAAATAGCAGGAGGTAGAGGCTACTATCTAAAAGGAGCTGGTGTGCGTTTGAATCAAGCTTTGATAAACTATGGCCTTGACTTTTTGGAGAAAAGGGGTTATACAGAACTGCAGACTCCTTTCTTCATGAGAAAAGATATCATGGCTAGATGTGCTCAATTGGCACAATTTGACGAAGAACTTTATAAGGTTACTGGTGAGGGTGATGATAAATATCTAATTGCCACCGCTGAACAACCACTTTGTTCATATCATCTTGAAGATTGGATTCATCCTTCTCAATTGCCTATAAGGTACGCTGGATATTCATCTTGCTTTCGTAAAGAAGCAGGTTCCCATGGCCGAGATACTCTTGGAATCTTCCGAGTCCACCAATTCGAGAAAGTAGAGCAATTTTGTATCACCAGCCCTAACGACAATGATTCGTGGGACATGCATGAAGAGATGATAAAAAATTCCGAGGAATTCTACAAGACGTTAAATATCCCATACCAAGTTGTGGCTATAGTTTCTGGTGCATTAAATGATGCAGCTGCCAAGAAATATGACTTAGAAGCATGGTTTCCTGCATCTCAGACTTACAGAGAGTTAGTATCATGTTCTAATTGCACTGACTATCAGTCCAGAAGGTTAGAGATTCGATACGGTCAGAAAAAGAGCAACGAGCAGACGAAACAGTATGTGCATTTGCTAAATTCTACACTCACCGCAACAGAAAGGACTCTATGCTGCATTCTTGAGAACTACCAAAAAGAAGATGGGGTTGAAGTACCTGAAGTTCTACGACCATATTTGGGTGGAAAGGCATTTCTACCCTTCAAAACAAAACCGACAGGGAGCGATTCCAAAGGGAAGAAATCGAAGGCCTAA

Coding sequence (CDS)

ATGTTGGACATCAATTTGTTCAGAGAAGAGAAAGGCAACAATCCTGAAATCATCCGTGAGTCTCAACGCCGCCGATTTGCCAACGTTGAACTCGTTGATGAAGTCATTAAGCTTGATAAAGAATGGCGTCAACGCCAATTTGAGCTGGAGAATCTCAGAAAGGAGTTCAACAAGATCAACAAGCAAATTGCTCAGCTTAGAATAGCTGGTGAGGATGCGTCCGAGAGGATTAAAGACACTGAAGAAAATAAAAAGTTGTCTGCTGAGAAAGATGTTGAAGCTAAGGATGTGTTAAAGAAGTTGAACTCAAAACTGGAAATAATTGGAAACCTTGTGCATGATTCCGTTCCCGTCAGCAATGATGAGGCTAATAATGTTGTTATCAGATCGTGGGGTGAGAAACTAGTAAAACCGAAACTGAAGAATCATGTTGATCTTGTTGAGCTTCTTGAAATTGCAGATTCTAAAAGAGGTTCTGAAATAGCAGGAGGTAGAGGCTACTATCTAAAAGGAGCTGGTGTGCGTTTGAATCAAGCTTTGATAAACTATGGCCTTGACTTTTTGGAGAAAAGGGGTTATACAGAACTGCAGACTCCTTTCTTCATGAGAAAAGATATCATGGCTAGATGTGCTCAATTGGCACAATTTGACGAAGAACTTTATAAGGTTACTGGTGAGGGTGATGATAAATATCTAATTGCCACCGCTGAACAACCACTTTGTTCATATCATCTTGAAGATTGGATTCATCCTTCTCAATTGCCTATAAGGTACGCTGGATATTCATCTTGCTTTCGTAAAGAAGCAGGTTCCCATGGCCGAGATACTCTTGGAATCTTCCGAGTCCACCAATTCGAGAAAGTAGAGCAATTTTGTATCACCAGCCCTAACGACAATGATTCGTGGGACATGCATGAAGAGATGATAAAAAATTCCGAGGAATTCTACAAGACGTTAAATATCCCATACCAAGTTGTGGCTATAGTTTCTGGTGCATTAAATGATGCAGCTGCCAAGAAATATGACTTAGAAGCATGGTTTCCTGCATCTCAGACTTACAGAGAGTTAGTATCATGTTCTAATTGCACTGACTATCAGTCCAGAAGGTTAGAGATTCGATACGGTCAGAAAAAGAGCAACGAGCAGACGAAACAGTATGTGCATTTGCTAAATTCTACACTCACCGCAACAGAAAGGACTCTATGCTGCATTCTTGAGAACTACCAAAAAGAAGATGGGGTTGAAGTACCTGAAGTTCTACGACCATATTTGGGTGGAAAGGCATTTCTACCCTTCAAAACAAAACCGACAGGGAGCGATTCCAAAGGGAAGAAATCGAAGGCCTAA
BLAST of CSPI05G00150.2 vs. Swiss-Prot
Match: SYSC_ARATH (Serine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana GN=At5g27470 PE=2 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 7.3e-210
Identity = 348/451 (77.16%), Postives = 409/451 (90.69%), Query Frame = 1

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+VE+VDE+IKLDKEWRQRQFE+++ RKEFNK+N
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVEIVDEIIKLDKEWRQRQFEVDSFRKEFNKLN 60

Query: 61  KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120
           KQ+AQL+I  EDASE I+ TE+NK+ S  K+ E ++    L +KLE +GNLVHDSVPV  
Sbjct: 61  KQVAQLKIKKEDASEIIQQTEKNKQDSTAKEAEVREAYAALKAKLEQVGNLVHDSVPVDK 120

Query: 121 DEANNVVIRSWGEKLVKP---KLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLN 180
           DEANN+VI+ WGEK       KLKNHVDLVELL IAD+KRG+EIAG RG++LKG G+ LN
Sbjct: 121 DEANNLVIKLWGEKRFSTPGLKLKNHVDLVELLGIADTKRGAEIAGARGFFLKGDGLMLN 180

Query: 181 QALINYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAE 240
           QALIN+GL FL+KRG+T LQ PFFMRKD+MA+CAQLAQFDEELYKVTGEGDDKYLIATAE
Sbjct: 181 QALINFGLTFLKKRGFTGLQPPFFMRKDVMAKCAQLAQFDEELYKVTGEGDDKYLIATAE 240

Query: 241 QPLCSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPN 300
           QPLC+YH+++WIHP++LP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEK+EQFCIT PN
Sbjct: 241 QPLCAYHIDEWIHPTELPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKIEQFCITGPN 300

Query: 301 DNDSWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELV 360
           +N SW+M +EM+KNSE+FY+ L +PYQ+V+IVSGALNDAAAKKYDLEAWFP+S+T+RELV
Sbjct: 301 ENASWEMLDEMMKNSEDFYQALKLPYQIVSIVSGALNDAAAKKYDLEAWFPSSETFRELV 360

Query: 361 SCSNCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVP 420
           SCSNCTDYQ+RRLEIRYGQKKSNEQTKQYVH+LNSTLTATERT+CCILENYQ+EDGV++P
Sbjct: 361 SCSNCTDYQARRLEIRYGQKKSNEQTKQYVHMLNSTLTATERTICCILENYQREDGVDIP 420

Query: 421 EVLRPYLGGKAFLPFKTKPTGSDSKGKKSKA 449
           EVL+P++GG+ FLPFK KP  +D+KGKKSKA
Sbjct: 421 EVLQPFMGGETFLPFKAKPVVADTKGKKSKA 451

BLAST of CSPI05G00150.2 vs. Swiss-Prot
Match: SYS_HELAN (Serine--tRNA ligase OS=Helianthus annuus PE=2 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 1.7e-206
Identity = 346/435 (79.54%), Postives = 391/435 (89.89%), Query Frame = 1

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKG NPEI+RESQRRR A+VE+VDE+IKLDKEWRQRQFELE LRK+FN+IN
Sbjct: 1   MLDINLFREEKGGNPEIVRESQRRRGASVEIVDEIIKLDKEWRQRQFELEQLRKDFNRIN 60

Query: 61  KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120
           K++A+LRI+G DAS  IK+TEENK  +A+K  E ++    L SKLE +GNLVHDSVPVSN
Sbjct: 61  KEVAKLRISGGDASSLIKNTEENKDSTAKKQAEVQEARTALYSKLETVGNLVHDSVPVSN 120

Query: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQAL 180
           DEA+N V+R+WGE+  +  LKNHV+LVELL IAD K+G+ +AGGRGYYLKG GVRLNQAL
Sbjct: 121 DEADNAVVRTWGERREETNLKNHVELVELLGIADLKKGANVAGGRGYYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           IN+GLDFLEKR YT LQTPFFMRKDIM +CAQLAQFDEELYKVTGEGDDKYLIATAEQPL
Sbjct: 181 INFGLDFLEKRKYTALQTPFFMRKDIMGKCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDND 300
           C+YH++DWIHP++LP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFC+T P+ ND
Sbjct: 241 CAYHIDDWIHPNELPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCLTKPSGND 300

Query: 301 SWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SW+MHEEMIKNSEEFY+ L +PYQVV+IVSGALNDAAAKKYDLEAWFPAS+TYRELVSCS
Sbjct: 301 SWEMHEEMIKNSEEFYQMLKLPYQVVSIVSGALNDAAAKKYDLEAWFPASKTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420
           NCTDYQSR+LEIR GQKK NEQ KQY HLLNSTLTATERTLCCILENYQ E GV +PEVL
Sbjct: 361 NCTDYQSRKLEIRCGQKKGNEQAKQYCHLLNSTLTATERTLCCILENYQTEKGVAIPEVL 420

Query: 421 RPYLGGKAFLPFKTK 436
           +P++GGK FL F  K
Sbjct: 421 QPFMGGKTFLDFLKK 435

BLAST of CSPI05G00150.2 vs. Swiss-Prot
Match: SYSC_DICDI (Serine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum GN=serS PE=1 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 1.6e-143
Identity = 253/431 (58.70%), Postives = 319/431 (74.01%), Query Frame = 1

Query: 2   LDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKINK 61
           LDI LFR +KG NP++IR+SQ+ R+ANV+ V EVI LDK  ++ ++ L+N   E+ K+NK
Sbjct: 3   LDIVLFRADKGGNPDLIRQSQKVRYANVDAVQEVIDLDKVVKETKYRLDNTNAEYAKLNK 62

Query: 62  QIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSND 121
            +A  + AGE A E I   EE  +   +   E  +V   L  KL  IGN+VH+SVP+ N+
Sbjct: 63  SVAMKKKAGESADEIIAQAEELNQSIIKLKAEVSEVELLLRKKLRPIGNIVHESVPIDNN 122

Query: 122 EANNVVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQALI 181
           E NN +I++WGE      L +H +L+E+++  D +RG+ ++G R Y+LKG GV LNQA+I
Sbjct: 123 EDNNQIIKTWGECKTSEGLLHHHELLEMIDGYDPERGTLVSGHRCYFLKGIGVLLNQAII 182

Query: 182 NYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPLC 241
           N+ L  + KRG   LQTPFFM KD+MA+ AQL QFD+ELYKVTG+ ++KYLIAT+EQP+ 
Sbjct: 183 NFALMHMTKRGSVPLQTPFFMNKDVMAKTAQLEQFDDELYKVTGDNEEKYLIATSEQPIS 242

Query: 242 SYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDNDS 301
           ++H ++WI    LP +Y GYS+CFRKEAGSHGRDT GIFRVHQFEK+EQFCIT P    S
Sbjct: 243 AFHQDEWIEEKDLPKKYVGYSTCFRKEAGSHGRDTWGIFRVHQFEKIEQFCITEP--EKS 302

Query: 302 WDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSN 361
           WDM EEMI NSE+FY+ L IPY+VV IVSGALN+AA+KKYDLE WFP    YRELVSCSN
Sbjct: 303 WDMMEEMINNSEQFYQELGIPYRVVNIVSGALNNAASKKYDLEGWFPGYNQYRELVSCSN 362

Query: 362 CTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVLR 421
           CTDYQSR LEIR G KK  +Q K+YVH+LNSTL AT R +CCILENYQ E G+ VP  LR
Sbjct: 363 CTDYQSRDLEIRCGMKKQGQQQKKYVHMLNSTLAATTRVICCILENYQTEGGITVPVPLR 422

Query: 422 PYLGGKAFLPF 433
           PYL GK F+PF
Sbjct: 423 PYL-GKDFIPF 430

BLAST of CSPI05G00150.2 vs. Swiss-Prot
Match: SYSC_SCHPO (Serine--tRNA ligase, cytoplasmic OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPAC29A4.15 PE=3 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 9.5e-141
Identity = 263/456 (57.68%), Postives = 333/456 (73.03%), Query Frame = 1

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLF+ EKG NPEIIRESQR+R A+V +VD+VI++ KEW   +FEL+N  K  N++ 
Sbjct: 1   MLDINLFQVEKGGNPEIIRESQRKRGADVGVVDKVIEMYKEWVSLRFELDNTNKSINRVQ 60

Query: 61  KQIAQLRIAGEDASERIKD----TEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSV 120
           K+I     A EDASE +++    TE  K L  ++  + K++L  ++S    IGN+VHDSV
Sbjct: 61  KEIGLKMKAKEDASELLEEKNSLTERKKNLIEQETAKNKEMLNVVSS----IGNIVHDSV 120

Query: 121 PVSNDEANNVVIRSWGEKLVKPKLKN---HVDLVELLEIADSKRGSEIAGGRGYYLKGAG 180
           PVS DE NN +IR W  + V  + KN   H +++  L+  D +RG +++G RGY+L+  G
Sbjct: 121 PVSMDEDNNEIIRKWAPEGVTVEKKNCLSHHEVLTRLDGYDPERGVKVSGHRGYFLRQYG 180

Query: 181 VRLNQALINYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLI 240
           V  N ALI YGLDFLEKRGY  LQ P  + KD+MA+ AQL QFDEELYKV    +++YLI
Sbjct: 181 VFFNLALIQYGLDFLEKRGYIALQAPTMLNKDVMAKTAQLEQFDEELYKVIDGDEERYLI 240

Query: 241 ATAEQPLCSYHLEDWIH-PS-QLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQF 300
           AT+EQP+ +YH  +W   PS QLP++YAGYS+C+R+EAGSHGRD  GIFRVH FEK+EQF
Sbjct: 241 ATSEQPISAYHSGEWFEKPSEQLPLKYAGYSTCYRREAGSHGRDAWGIFRVHAFEKIEQF 300

Query: 301 CITSPNDNDSWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQ 360
            +T P    SW+   EMI ++E+FYK+L +PY++VAIVSGALN+AAAKKYDLEAWFP   
Sbjct: 301 VLTDPEK--SWEAFTEMINHAEDFYKSLELPYRIVAIVSGALNNAAAKKYDLEAWFPFQG 360

Query: 361 TYRELVSCSNCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKE 420
            Y+ELVSCSNCTDYQSR LEIR G KK  ++ K+YVH LNSTL ATER LCCILENYQ  
Sbjct: 361 EYKELVSCSNCTDYQSRNLEIRCGVKKMGDREKKYVHCLNSTLCATERALCCILENYQTP 420

Query: 421 DGVEVPEVLRPYLGGKAFLPFKTKPTGSDSKGKKSK 448
           DGV VP+VL+PY+GGK FLPF TK    +S  KK K
Sbjct: 421 DGVNVPKVLQPYMGGKTFLPF-TKELPKNSTSKKGK 449

BLAST of CSPI05G00150.2 vs. Swiss-Prot
Match: SYSC_YEAST (Serine--tRNA ligase, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SES1 PE=1 SV=2)

HSP 1 Score: 472.2 bits (1214), Expect = 6.1e-132
Identity = 253/467 (54.18%), Postives = 324/467 (69.38%), Query Frame = 1

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDIN F E+KG NPE+IR+SQ+ R A+VE+VDE+I   K+W + +FEL+ L K+FNK+ 
Sbjct: 1   MLDINQFIEDKGGNPELIRQSQKARNASVEIVDEIISDYKDWVKTRFELDELNKKFNKLQ 60

Query: 61  KQIAQLRIAGEDAS----ERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSV 120
           K I       EDAS    E+ K T++ K+L+ ++  E KD+ KK+      +GN+VH SV
Sbjct: 61  KDIGLKFKNKEDASGLLAEKEKLTQQKKELTEKEQQEDKDLKKKVFQ----VGNIVHPSV 120

Query: 121 PVSNDEANNVVIRSWGEKLV-----------KPKLKNHVDLVELLEIADSKRGSEIAGGR 180
            VSNDE NN ++R+W  + +           KP   +H +++  L+  D  RG +I G R
Sbjct: 121 VVSNDEENNELVRTWKPEDLEAVGPIASVTGKPASLSHHEILLRLDGYDPDRGVKICGHR 180

Query: 181 GYYLKGAGVRLNQALINYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTG 240
           GY+ +  GV LNQALINYGL FL  +GY  LQ P  M K++M++ AQL++FDEELYKV  
Sbjct: 181 GYFFRNYGVFLNQALINYGLQFLAAKGYIPLQAPVMMNKELMSKTAQLSEFDEELYKVID 240

Query: 241 EGDDKYLIATAEQPLCSYHLEDWIHP--SQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVH 300
             D+KYLIAT+EQP+ +YH  +W      QLPI Y GYSSCFR+EAGSHG+D  G+FRVH
Sbjct: 241 GEDEKYLIATSEQPISAYHSGEWFEKPQEQLPIHYVGYSSCFRREAGSHGKDAWGVFRVH 300

Query: 301 QFEKVEQFCITSPNDNDSWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDL 360
            FEK+EQF IT P    SW+  E+MI  SEEFYK+L +PY++V IVSG LN+AAAKKYDL
Sbjct: 301 AFEKIEQFVITEPEK--SWEEFEKMISYSEEFYKSLKLPYRIVGIVSGELNNAAAKKYDL 360

Query: 361 EAWFPASQTYRELVSCSNCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCC 420
           EAWFP  + Y+ELVSCSNCTDYQSR LEIR G KK  ++ K+YVH LNSTL AT+R LCC
Sbjct: 361 EAWFPYQKEYKELVSCSNCTDYQSRNLEIRCGIKKMGDREKKYVHCLNSTLAATQRALCC 420

Query: 421 ILENYQKEDGVEVPEVLRPYLGGK-AFLPF--KTKPTGSDSKGKKSK 448
           ILENYQ EDG+ VPEVLR Y+ G+  FLPF  +     + SK KK K
Sbjct: 421 ILENYQTEDGLVVPEVLRKYIPGEPEFLPFVNELPKNSTSSKDKKKK 461

BLAST of CSPI05G00150.2 vs. TrEMBL
Match: A0A0A0KL58_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G002590 PE=4 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 1.1e-257
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 1

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQAL 180
           DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQAL
Sbjct: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL
Sbjct: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDND 300
           CSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDND
Sbjct: 241 CSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDND 300

Query: 301 SWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420
           NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL
Sbjct: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420

Query: 421 RPYLGGKAFLPFKTKPTGSDSKGKKSKA 449
           RPYLGGKAFLPFKTKPTGSDSKGKKSKA
Sbjct: 421 RPYLGGKAFLPFKTKPTGSDSKGKKSKA 448

BLAST of CSPI05G00150.2 vs. TrEMBL
Match: M5VY64_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005710mg PE=4 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 2.4e-228
Identity = 387/448 (86.38%), Postives = 427/448 (95.31%), Query Frame = 1

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRF  VE+VDEVI+LDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFKAVEIVDEVIQLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120
           K++AQLRIAGEDA+  IKDTEENK+L+AEK+VE ++ L +LNSKLE+IGNLVHDSVP+SN
Sbjct: 61  KRVAQLRIAGEDATAVIKDTEENKRLAAEKEVEVREALTQLNSKLEVIGNLVHDSVPISN 120

Query: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQAL 180
           DE NN VIRSWGEK ++PKLKNHV+LVELL IAD K+G+E+AGGRG+YLKGAGVRLNQAL
Sbjct: 121 DEENNAVIRSWGEKRMEPKLKNHVELVELLGIADLKKGAEVAGGRGFYLKGAGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           IN+GLDFLEKRGYTELQTPFFMRKDIMA+CAQLAQFDEELYKVTGEGDDKYLIATAEQPL
Sbjct: 181 INFGLDFLEKRGYTELQTPFFMRKDIMAKCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDND 300
           CSYH++DWIHPSQLP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSP+ +D
Sbjct: 241 CSYHIDDWIHPSQLPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPDGSD 300

Query: 301 SWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SW+MHEEMIKNSE+FYK+LN+PY VVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWEMHEEMIKNSEDFYKSLNLPYHVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420
           NCTDYQSR+LE RYGQKKSNEQTK+YVHLLNSTLTATERTLCCILENYQ+EDGV++PEVL
Sbjct: 361 NCTDYQSRKLETRYGQKKSNEQTKKYVHLLNSTLTATERTLCCILENYQREDGVDIPEVL 420

Query: 421 RPYLGGKAFLPFKTKPTGSDSKGKKSKA 449
           +P++GGK FLPFK KP   + KGKKSKA
Sbjct: 421 QPFMGGKTFLPFKNKP-APEVKGKKSKA 447

BLAST of CSPI05G00150.2 vs. TrEMBL
Match: D7U1Z6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0128g00370 PE=4 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 5.4e-228
Identity = 390/448 (87.05%), Postives = 423/448 (94.42%), Query Frame = 1

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFRE+KG+NPEIIRESQRRR+ANVELVDEVI LDKEWRQRQFE ENLRKEFNKIN
Sbjct: 1   MLDINLFREDKGHNPEIIRESQRRRYANVELVDEVIHLDKEWRQRQFEYENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120
           KQ+A+L+I+GEDASE IK T+ENK+L+++K+ E ++ L  LNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQVAKLKISGEDASEMIKSTDENKRLTSKKEAEVQEALAALNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQAL 180
           DEANN VIRSWGEK V+PKLKNHV+LVELL IAD K+G+ IAGGRG+YLKG GVRLNQAL
Sbjct: 121 DEANNAVIRSWGEKKVEPKLKNHVELVELLGIADLKKGANIAGGRGFYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           IN+GLDFLEKRGYT LQTPFFMRKDIMA+CAQLAQFDEELYKVTGEGDDKYLIATAEQPL
Sbjct: 181 INFGLDFLEKRGYTALQTPFFMRKDIMAKCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDND 300
           C+YHL+DWIHPSQLP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPN ND
Sbjct: 241 CAYHLDDWIHPSQLPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGND 300

Query: 301 SWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWDMHEEMIKNSE+FYK LNIPYQVVAIVSGALNDAAAKKYDLE WFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKMLNIPYQVVAIVSGALNDAAAKKYDLEGWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420
           NCTDYQSRRLEIRYGQKKSNEQ KQYVHLLNSTLTATERT+CCILENYQKEDGVEVPE L
Sbjct: 361 NCTDYQSRRLEIRYGQKKSNEQIKQYVHLLNSTLTATERTICCILENYQKEDGVEVPEPL 420

Query: 421 RPYLGGKAFLPFKTKPTGSDSKGKKSKA 449
           RP++GGK FLPF+T P  +++KGKKSKA
Sbjct: 421 RPFMGGKTFLPFQTSP-ATEAKGKKSKA 447

BLAST of CSPI05G00150.2 vs. TrEMBL
Match: W9RWJ2_9ROSA (Serine--tRNA ligase OS=Morus notabilis GN=L484_001167 PE=4 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 1.4e-223
Identity = 381/448 (85.04%), Postives = 419/448 (93.53%), Query Frame = 1

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKG NPEIIRESQRRRFANV++VDE+IKLDKEWRQRQFE ENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGGNPEIIRESQRRRFANVDIVDEIIKLDKEWRQRQFEFENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120
           KQ+AQLRI+G DA+E IK+TE++KK  AEK+VE K+ LK+LNS LEIIGNLVHDSVP+SN
Sbjct: 61  KQVAQLRISGGDATEMIKNTEDHKKSIAEKEVEVKEALKQLNSILEIIGNLVHDSVPISN 120

Query: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQAL 180
           DEANN VIR WGEK ++PKLKNHV+LVELL IAD+K+G+++AGGRG+YLKG GV LNQAL
Sbjct: 121 DEANNAVIRLWGEKRLEPKLKNHVELVELLGIADTKKGTDVAGGRGFYLKGDGVHLNQAL 180

Query: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           IN+GL FLEKR +T L TPFFMRKDIMA+CAQLAQFDEELYKVTGEGDDKYLIATAEQPL
Sbjct: 181 INFGLQFLEKREFTALHTPFFMRKDIMAKCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDND 300
           C+YHL+DWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFC+TSPN ND
Sbjct: 241 CAYHLDDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCLTSPNGND 300

Query: 301 SWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWDMHEEMIKNSE+FYK L IPYQVV+IVSGALNDAAAKKYDLE WFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKMLKIPYQVVSIVSGALNDAAAKKYDLEGWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420
           NCTDYQSRRLEIR+GQKK+N+QTKQYVHLLNSTLTATERTLCCILENYQKEDGVE+PE L
Sbjct: 361 NCTDYQSRRLEIRFGQKKANDQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEAL 420

Query: 421 RPYLGGKAFLPFKTKPTGSDSKGKKSKA 449
           + Y+GGK FLPFK KP  S++KGKKSKA
Sbjct: 421 QGYMGGKTFLPFKNKP-ASETKGKKSKA 447

BLAST of CSPI05G00150.2 vs. TrEMBL
Match: A0A059B6V5_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H04482 PE=4 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 6.9e-223
Identity = 376/448 (83.93%), Postives = 420/448 (93.75%), Query Frame = 1

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFANV++VDE+I LDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVDIVDEIINLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120
           K++AQL+I+GEDASE I +T ENKKL+AEK+VE ++    L S+LEI+GNLVHDSVPV+N
Sbjct: 61  KRVAQLKISGEDASEMIANTNENKKLTAEKEVEVREAWMALKSRLEIVGNLVHDSVPVNN 120

Query: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQAL 180
           DEANN V+R+WG+K V+PKLKNHV+LV+LLEIAD K+G+++AG RG+YLKG GVRLNQAL
Sbjct: 121 DEANNAVVRTWGDKRVEPKLKNHVELVDLLEIADLKKGADVAGARGFYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           I +GLDFLEKRGYT LQTPFFM++DIMA+CAQLAQFDEELYKVTGEGDDKYLIATAEQPL
Sbjct: 181 ICFGLDFLEKRGYTALQTPFFMKQDIMAKCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDND 300
           C+YHL+DWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCIT+PN N+
Sbjct: 241 CAYHLDDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITTPNGNE 300

Query: 301 SWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWDMHEEMIKNSEEFY+ L IPY V+AIVSGALNDAAAKKYDLE WFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEEFYQALKIPYHVIAIVSGALNDAAAKKYDLEGWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420
           NCTDYQ+RRLEIRYGQKKSNEQ KQYVHLLNSTLTATERT+CCILENYQ+EDGVEVPEVL
Sbjct: 361 NCTDYQARRLEIRYGQKKSNEQVKQYVHLLNSTLTATERTICCILENYQREDGVEVPEVL 420

Query: 421 RPYLGGKAFLPFKTKPTGSDSKGKKSKA 449
           R Y+GGK FLPFK KP  +++KGKK+KA
Sbjct: 421 RGYMGGKTFLPFKAKP-ATETKGKKTKA 447

BLAST of CSPI05G00150.2 vs. TAIR10
Match: AT5G27470.1 (AT5G27470.1 seryl-tRNA synthetase / serine--tRNA ligase)

HSP 1 Score: 731.1 bits (1886), Expect = 4.1e-211
Identity = 348/451 (77.16%), Postives = 409/451 (90.69%), Query Frame = 1

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+VE+VDE+IKLDKEWRQRQFE+++ RKEFNK+N
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVEIVDEIIKLDKEWRQRQFEVDSFRKEFNKLN 60

Query: 61  KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120
           KQ+AQL+I  EDASE I+ TE+NK+ S  K+ E ++    L +KLE +GNLVHDSVPV  
Sbjct: 61  KQVAQLKIKKEDASEIIQQTEKNKQDSTAKEAEVREAYAALKAKLEQVGNLVHDSVPVDK 120

Query: 121 DEANNVVIRSWGEKLVKP---KLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLN 180
           DEANN+VI+ WGEK       KLKNHVDLVELL IAD+KRG+EIAG RG++LKG G+ LN
Sbjct: 121 DEANNLVIKLWGEKRFSTPGLKLKNHVDLVELLGIADTKRGAEIAGARGFFLKGDGLMLN 180

Query: 181 QALINYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAE 240
           QALIN+GL FL+KRG+T LQ PFFMRKD+MA+CAQLAQFDEELYKVTGEGDDKYLIATAE
Sbjct: 181 QALINFGLTFLKKRGFTGLQPPFFMRKDVMAKCAQLAQFDEELYKVTGEGDDKYLIATAE 240

Query: 241 QPLCSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPN 300
           QPLC+YH+++WIHP++LP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEK+EQFCIT PN
Sbjct: 241 QPLCAYHIDEWIHPTELPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKIEQFCITGPN 300

Query: 301 DNDSWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELV 360
           +N SW+M +EM+KNSE+FY+ L +PYQ+V+IVSGALNDAAAKKYDLEAWFP+S+T+RELV
Sbjct: 301 ENASWEMLDEMMKNSEDFYQALKLPYQIVSIVSGALNDAAAKKYDLEAWFPSSETFRELV 360

Query: 361 SCSNCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVP 420
           SCSNCTDYQ+RRLEIRYGQKKSNEQTKQYVH+LNSTLTATERT+CCILENYQ+EDGV++P
Sbjct: 361 SCSNCTDYQARRLEIRYGQKKSNEQTKQYVHMLNSTLTATERTICCILENYQREDGVDIP 420

Query: 421 EVLRPYLGGKAFLPFKTKPTGSDSKGKKSKA 449
           EVL+P++GG+ FLPFK KP  +D+KGKKSKA
Sbjct: 421 EVLQPFMGGETFLPFKAKPVVADTKGKKSKA 451

BLAST of CSPI05G00150.2 vs. TAIR10
Match: AT1G11870.2 (AT1G11870.2 Seryl-tRNA synthetase)

HSP 1 Score: 250.8 bits (639), Expect = 1.6e-66
Identity = 156/439 (35.54%), Postives = 248/439 (56.49%), Query Frame = 1

Query: 13  NNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKINKQIAQLRIAGED 72
           +N E +  + R R +N  L + V++L +     Q E+E LR+E N + K++        +
Sbjct: 86  DNKEAVEINIRNRNSNANL-EAVLQLYENMVNLQKEVERLREERNNVAKKMK----GKLE 145

Query: 73  ASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIG----NLVHDSVPVSNDEANNVVI 132
            SER +  EE K L  E  V  ++ L KL  +L+ +     N+ H  VPV  ++++ +  
Sbjct: 146 PSERERLVEEGKNLK-ESLVTLEEDLVKLKDELQHVAQSIPNMTHPDVPVGGEDSSAIRQ 205

Query: 133 RSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQALINYGLDFL 192
                +     +K+H+ L + L++ D    +E++G + +YLK   V L  AL+N+ L  +
Sbjct: 206 EVGSPREFSFPIKDHLQLGKDLDLIDFDSAAEVSGSKFFYLKNEAVLLEMALLNWTLSQV 265

Query: 193 EKRGYTELQTPFFMRKDIMARCAQLAQFDE-ELYKVTGEGDDKYLIATAEQPLCSYHLED 252
            K+GYT L TP  +R  I+ +C    + D  ++Y +  +G D+ LI TAE P+   H++ 
Sbjct: 266 MKKGYTPLTTPEIVRSSIVEKCGFQPRGDNTQVYSI--DGTDQCLIGTAEIPVGGIHMDS 325

Query: 253 WIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDNDSWDMHEE 312
            +  S LP++Y  +S CFR EAG+ G  T G++RVHQF K E F I  P D++S+  H+E
Sbjct: 326 ILLESALPLKYIAFSHCFRTEAGAAGAATKGLYRVHQFSKAEMFVICQPEDSESF--HQE 385

Query: 313 MIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDYQS 372
           +I+  E+ + +L + ++ + + +  L   A +K+D+EAW P    + E+ S SNCTDYQS
Sbjct: 386 LIQIEEDLFTSLGLHFKTLDMATADLGAPAYRKFDIEAWMPGLGRFGEISSASNCTDYQS 445

Query: 373 RRLEIRY--------GQK--KSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDG-VEV 432
           RRL IRY        G K  K+N    ++VH LN+T  A  R + C+LENYQ+EDG V +
Sbjct: 446 RRLGIRYRPSEPPQTGPKKGKANLPATKFVHTLNATACAVPRMMVCLLENYQQEDGSVVI 505

Query: 433 PEVLRPYLGGKAFLPFKTK 436
           PE LRP++GG   +  K K
Sbjct: 506 PEPLRPFMGGIELIKPKHK 514

BLAST of CSPI05G00150.2 vs. NCBI nr
Match: gi|778697943|ref|XP_011654444.1| (PREDICTED: serine--tRNA ligase [Cucumis sativus])

HSP 1 Score: 896.7 bits (2316), Expect = 1.6e-257
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 1

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQAL 180
           DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQAL
Sbjct: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL
Sbjct: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDND 300
           CSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDND
Sbjct: 241 CSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDND 300

Query: 301 SWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420
           NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL
Sbjct: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420

Query: 421 RPYLGGKAFLPFKTKPTGSDSKGKKSKA 449
           RPYLGGKAFLPFKTKPTGSDSKGKKSKA
Sbjct: 421 RPYLGGKAFLPFKTKPTGSDSKGKKSKA 448

BLAST of CSPI05G00150.2 vs. NCBI nr
Match: gi|659129563|ref|XP_008464736.1| (PREDICTED: serine--tRNA ligase [Cucumis melo])

HSP 1 Score: 885.2 bits (2286), Expect = 4.9e-254
Identity = 441/448 (98.44%), Postives = 446/448 (99.55%), Query Frame = 1

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASERIKDTEENK+LSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASERIKDTEENKRLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQAL 180
           DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIAD+KRGSEIAGGRGYYLKGAGVRLNQAL
Sbjct: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADTKRGSEIAGGRGYYLKGAGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL
Sbjct: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDND 300
           CSYHLEDWIHPSQLP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPN ND
Sbjct: 241 CSYHLEDWIHPSQLPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGND 300

Query: 301 SWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWDMHEEMIKNSE+FYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420
           NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL
Sbjct: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420

Query: 421 RPYLGGKAFLPFKTKPTGSDSKGKKSKA 449
           RPYLGGKAFLPFKTKPT +DSKGKKSKA
Sbjct: 421 RPYLGGKAFLPFKTKPTVNDSKGKKSKA 448

BLAST of CSPI05G00150.2 vs. NCBI nr
Match: gi|645222203|ref|XP_008246480.1| (PREDICTED: serine--tRNA ligase [Prunus mume])

HSP 1 Score: 800.0 bits (2065), Expect = 2.1e-228
Identity = 386/448 (86.16%), Postives = 428/448 (95.54%), Query Frame = 1

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRF  VE+VDEVI+LDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFKGVEIVDEVIQLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120
           K++AQLRIAGEDA++ IKDTEENK+L+AEK++E ++ L +LNSKLE+IGNLVHDSVP+SN
Sbjct: 61  KRVAQLRIAGEDATDVIKDTEENKRLAAEKELEVREALTQLNSKLEVIGNLVHDSVPISN 120

Query: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQAL 180
           DE NN VIRSWGEK ++PKLKNHV+LVELL IAD K+G+E+AGGRG+YLKGAGVRLNQAL
Sbjct: 121 DEENNAVIRSWGEKRMEPKLKNHVELVELLGIADLKKGAEVAGGRGFYLKGAGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           IN+GLDFLEKRGYTELQTPFFMRKDIMA+CAQLAQFDEELYKVTGEGDDKYLIATAEQPL
Sbjct: 181 INFGLDFLEKRGYTELQTPFFMRKDIMAKCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDND 300
           CSYH++DWIHPSQLP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSP+ +D
Sbjct: 241 CSYHIDDWIHPSQLPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPDGSD 300

Query: 301 SWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SW+MHEEMIKNSE+FYK+LN+PY VVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWEMHEEMIKNSEDFYKSLNLPYHVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420
           NCTDYQSR+LE RYGQKKSNEQTK+YVHLLNSTLTATERTLCCILENYQ+EDGV++PEVL
Sbjct: 361 NCTDYQSRKLETRYGQKKSNEQTKKYVHLLNSTLTATERTLCCILENYQREDGVDIPEVL 420

Query: 421 RPYLGGKAFLPFKTKPTGSDSKGKKSKA 449
           +P++GGK FLPFK KP   + KGKKSKA
Sbjct: 421 QPFMGGKTFLPFKNKP-APEVKGKKSKA 447

BLAST of CSPI05G00150.2 vs. NCBI nr
Match: gi|595824204|ref|XP_007205198.1| (hypothetical protein PRUPE_ppa005710mg [Prunus persica])

HSP 1 Score: 799.3 bits (2063), Expect = 3.5e-228
Identity = 387/448 (86.38%), Postives = 427/448 (95.31%), Query Frame = 1

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRF  VE+VDEVI+LDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFKAVEIVDEVIQLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120
           K++AQLRIAGEDA+  IKDTEENK+L+AEK+VE ++ L +LNSKLE+IGNLVHDSVP+SN
Sbjct: 61  KRVAQLRIAGEDATAVIKDTEENKRLAAEKEVEVREALTQLNSKLEVIGNLVHDSVPISN 120

Query: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQAL 180
           DE NN VIRSWGEK ++PKLKNHV+LVELL IAD K+G+E+AGGRG+YLKGAGVRLNQAL
Sbjct: 121 DEENNAVIRSWGEKRMEPKLKNHVELVELLGIADLKKGAEVAGGRGFYLKGAGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           IN+GLDFLEKRGYTELQTPFFMRKDIMA+CAQLAQFDEELYKVTGEGDDKYLIATAEQPL
Sbjct: 181 INFGLDFLEKRGYTELQTPFFMRKDIMAKCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDND 300
           CSYH++DWIHPSQLP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSP+ +D
Sbjct: 241 CSYHIDDWIHPSQLPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPDGSD 300

Query: 301 SWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SW+MHEEMIKNSE+FYK+LN+PY VVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWEMHEEMIKNSEDFYKSLNLPYHVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420
           NCTDYQSR+LE RYGQKKSNEQTK+YVHLLNSTLTATERTLCCILENYQ+EDGV++PEVL
Sbjct: 361 NCTDYQSRKLETRYGQKKSNEQTKKYVHLLNSTLTATERTLCCILENYQREDGVDIPEVL 420

Query: 421 RPYLGGKAFLPFKTKPTGSDSKGKKSKA 449
           +P++GGK FLPFK KP   + KGKKSKA
Sbjct: 421 QPFMGGKTFLPFKNKP-APEVKGKKSKA 447

BLAST of CSPI05G00150.2 vs. NCBI nr
Match: gi|225450981|ref|XP_002284787.1| (PREDICTED: serine--tRNA ligase [Vitis vinifera])

HSP 1 Score: 798.1 bits (2060), Expect = 7.8e-228
Identity = 390/448 (87.05%), Postives = 423/448 (94.42%), Query Frame = 1

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFRE+KG+NPEIIRESQRRR+ANVELVDEVI LDKEWRQRQFE ENLRKEFNKIN
Sbjct: 1   MLDINLFREDKGHNPEIIRESQRRRYANVELVDEVIHLDKEWRQRQFEYENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASERIKDTEENKKLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120
           KQ+A+L+I+GEDASE IK T+ENK+L+++K+ E ++ L  LNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQVAKLKISGEDASEMIKSTDENKRLTSKKEAEVQEALAALNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADSKRGSEIAGGRGYYLKGAGVRLNQAL 180
           DEANN VIRSWGEK V+PKLKNHV+LVELL IAD K+G+ IAGGRG+YLKG GVRLNQAL
Sbjct: 121 DEANNAVIRSWGEKKVEPKLKNHVELVELLGIADLKKGANIAGGRGFYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           IN+GLDFLEKRGYT LQTPFFMRKDIMA+CAQLAQFDEELYKVTGEGDDKYLIATAEQPL
Sbjct: 181 INFGLDFLEKRGYTALQTPFFMRKDIMAKCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CSYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNDND 300
           C+YHL+DWIHPSQLP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPN ND
Sbjct: 241 CAYHLDDWIHPSQLPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGND 300

Query: 301 SWDMHEEMIKNSEEFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWDMHEEMIKNSE+FYK LNIPYQVVAIVSGALNDAAAKKYDLE WFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKMLNIPYQVVAIVSGALNDAAAKKYDLEGWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420
           NCTDYQSRRLEIRYGQKKSNEQ KQYVHLLNSTLTATERT+CCILENYQKEDGVEVPE L
Sbjct: 361 NCTDYQSRRLEIRYGQKKSNEQIKQYVHLLNSTLTATERTICCILENYQKEDGVEVPEPL 420

Query: 421 RPYLGGKAFLPFKTKPTGSDSKGKKSKA 449
           RP++GGK FLPF+T P  +++KGKKSKA
Sbjct: 421 RPFMGGKTFLPFQTSP-ATEAKGKKSKA 447

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SYSC_ARATH7.3e-21077.16Serine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana GN=At5g27470 PE=2 SV=1[more]
SYS_HELAN1.7e-20679.54Serine--tRNA ligase OS=Helianthus annuus PE=2 SV=1[more]
SYSC_DICDI1.6e-14358.70Serine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum GN=serS PE=1 SV=1[more]
SYSC_SCHPO9.5e-14157.68Serine--tRNA ligase, cytoplasmic OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
SYSC_YEAST6.1e-13254.18Serine--tRNA ligase, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204508... [more]
Match NameE-valueIdentityDescription
A0A0A0KL58_CUCSA1.1e-257100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G002590 PE=4 SV=1[more]
M5VY64_PRUPE2.4e-22886.38Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005710mg PE=4 SV=1[more]
D7U1Z6_VITVI5.4e-22887.05Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0128g00370 PE=4 SV=... [more]
W9RWJ2_9ROSA1.4e-22385.04Serine--tRNA ligase OS=Morus notabilis GN=L484_001167 PE=4 SV=1[more]
A0A059B6V5_EUCGR6.9e-22383.93Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H04482 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G27470.14.1e-21177.16 seryl-tRNA synthetase / serine--tRNA ligase[more]
AT1G11870.21.6e-6635.54 Seryl-tRNA synthetase[more]
Match NameE-valueIdentityDescription
gi|778697943|ref|XP_011654444.1|1.6e-257100.00PREDICTED: serine--tRNA ligase [Cucumis sativus][more]
gi|659129563|ref|XP_008464736.1|4.9e-25498.44PREDICTED: serine--tRNA ligase [Cucumis melo][more]
gi|645222203|ref|XP_008246480.1|2.1e-22886.16PREDICTED: serine--tRNA ligase [Prunus mume][more]
gi|595824204|ref|XP_007205198.1|3.5e-22886.38hypothetical protein PRUPE_ppa005710mg [Prunus persica][more]
gi|225450981|ref|XP_002284787.1|7.8e-22887.05PREDICTED: serine--tRNA ligase [Vitis vinifera][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR002314aa-tRNA-synt_IIb
IPR002317Ser-tRNA-ligase_type_1
IPR006195aa-tRNA-synth_II
IPR010978tRNA-bd_arm
IPR015866Ser-tRNA-synth_1_N
Vocabulary: Molecular Function
TermDefinition
GO:0000166nucleotide binding
GO:0004812aminoacyl-tRNA ligase activity
GO:0005524ATP binding
GO:0004828serine-tRNA ligase activity
Vocabulary: Biological Process
TermDefinition
GO:0006418tRNA aminoacylation for protein translation
GO:0006434seryl-tRNA aminoacylation
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006434 seryl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
biological_process GO:0006094 gluconeogenesis
biological_process GO:0006544 glycine metabolic process
biological_process GO:0006096 glycolytic process
biological_process GO:0006563 L-serine metabolic process
biological_process GO:0006499 N-terminal protein myristoylation
biological_process GO:0009165 nucleotide biosynthetic process
biological_process GO:0009651 response to salt stress
biological_process GO:0006566 threonine metabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005829 cytosol
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0004828 serine-tRNA ligase activity

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI05G00150CSPI05G00150gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI05G00150.2CSPI05G00150.2-proteinpolypeptide


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI05G00150.2.utr3p1CSPI05G00150.2.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI05G00150.2.cds9CSPI05G00150.2.cds9CDS
CSPI05G00150.2.cds8CSPI05G00150.2.cds8CDS
CSPI05G00150.2.cds7CSPI05G00150.2.cds7CDS
CSPI05G00150.2.cds6CSPI05G00150.2.cds6CDS
CSPI05G00150.2.cds5CSPI05G00150.2.cds5CDS
CSPI05G00150.2.cds4CSPI05G00150.2.cds4CDS
CSPI05G00150.2.cds3CSPI05G00150.2.cds3CDS
CSPI05G00150.2.cds2CSPI05G00150.2.cds2CDS
CSPI05G00150.2.cds1CSPI05G00150.2.cds1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI05G00150.2.utr5p1CSPI05G00150.2.utr5p1five_prime_UTR


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002314Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)PFAMPF00587tRNA-synt_2bcoord: 221..408
score: 5.1
IPR002317Serine-tRNA ligase, type1PRINTSPR00981TRNASYNTHSERcoord: 357..373
score: 4.0E-32coord: 280..293
score: 4.0E-32coord: 339..355
score: 4.0E-32coord: 268..280
score: 4.0E-32coord: 322..335
score: 4.0
IPR002317Serine-tRNA ligase, type1PIRPIRSF001529Ser-tRNA_ligasecoord: 1..433
score: 5.1E
IPR002317Serine-tRNA ligase, type1PANTHERPTHR11778SERYL-TRNA SYNTHETASEcoord: 1..446
score: 2.3E
IPR002317Serine-tRNA ligase, type1TIGRFAMsTIGR00414TIGR00414coord: 1..424
score: 3.5E
IPR006195Aminoacyl-tRNA synthetase, class IIPROFILEPS50862AA_TRNA_LIGASE_IIcoord: 142..422
score: 23
IPR010978tRNA-binding armunknownSSF46589tRNA-binding armcoord: 1..117
score: 1.05
IPR015866Serine-tRNA synthetase, type1, N-terminalGENE3DG3DSA:1.10.287.40coord: 1..105
score: 3.0
IPR015866Serine-tRNA synthetase, type1, N-terminalPFAMPF02403Seryl_tRNA_Ncoord: 1..112
score: 7.2
NoneNo IPR availableunknownCoilCoilcoord: 49..83
scor
NoneNo IPR availableGENE3DG3DSA:3.30.930.10coord: 110..427
score: 2.7E
NoneNo IPR availablePANTHERPTHR11778:SF7SERINE--TRNA LIGASE, CYTOPLASMICcoord: 1..446
score: 2.3E
NoneNo IPR availableunknownSSF55681Class II aaRS and biotin synthetasescoord: 121..431
score: 1.05