CSPI04G01710.1 (mRNA) Wild cucumber (PI 183967)

NameCSPI04G01710.1
TypemRNA
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionDelta-9 fatty acid desaturase
LocationChr4 : 954751 .. 957358 (-)
Sequence length1026
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTGAGGCAGTCTTTGCTTTTAAAACGTTCCTATACTACTCTTTATCGATCTTTGTTTTTTTATTTTGCCATTACTCTCTCTTACTCCTCAAATGGAGGCCACAAGGAAAGAGCTGGAAGATAAAAATAGCGATCAGAAGGCTATGGCGGTTGAAGACAAACCTCGGGCTAGATCCCGGCGAAGACCATTTTTCCGAAGAAAATGGACAACTTTAGACAAACGTTCTGCTGGTGCTTTTCTTGTTATGCATTTTCTCTGTGTTTTGGCACCATTTTATTTCACATGGCCAGCCTTCTGGCTTGCCTTTGTACTCTACATTATCACGGGCTTGTTCGGTTTGACACTCTCGTACCACCGGCATCTTACACACAAGAGTTTCAAACTTCCCAAATGGCTTGAGTACACGTTTGCTTACATTGGAGTCCATACACTTCAAGTACACCTTCACTTTTTTTTTTTTCTTTTTTAATTGTTTTCTTGACAATGTTTAAATTATAATCTATTTTTCGGTGTGTTTATAGGGCGATCCGATTGATTGGGTGAGTACGCATAGATATCACCATCAGTTTGTGGATACAAAAAGAGATCCACACACTCCGACACAAGGGTTTTTATTTGGACATATTGCTTGGATATTTGATTCATATGGTTTGACTAAAAAAGTTAGCCCTAAACATGTTGAAGATTTCGAAGCAAAAGATGAGAAAAGAAAGATTAGTAGAAATATGTTTGTGGGATATTTGAAGTATGGAAGACCTAACAATGCTGAGGATTTACAAAATCAAGGCTTTTACAGGTTTCTTCGAAGAACATACATTCTTCATCATTTTGGTCTTGCAATAATCCTCTATGCTCTTGGAGGGCTTCCTTTTCTCACTTGGGGAATGGTAAACCCAGTTATTCTAGCACTAATTTTGTTATTTTTTATTTTAAGTCGAGTTGATCCAAAAAACATCATTTTCAAATGTTAATTTTTAATATATACTTCTAGGTCAGAAGCTCACGTAAGAATTACAAATGTGTCAACTTAATTATATAAATCAATCTCCTGAAATAATTTACTTGTCATATTTTGTTTTAGAAAAACAAAAGTCAAATCAAACACTCTTAATTAGATCTAAAAATTTTATACTTGGGTTTGTCCCGGTTACAATTTAAAATCCTATTACTATTATCATGATCATTAGAAGTAATTTTAAATACAGTGAAATGAAATAAAATATTTACAAAATATAATAAAATTTTAACTCTATTATTGATAGACAGTCATATATTTATATCTCTCATCATCGATAGAATATGAAGATTTAGTATATTTTAGAAATGTTTTCTAATAGTTTTATCATTTACAATATTATTTAATGTTATTATTATTACTACTACATGTTAGCTTAAAATCTAACGTACACAAGTTGAAATATATGTGTCAACCTAGTTAACGTGTGTTCTATATGTGTCTAAGTTATTTGACATATGTTAGTGTGTCGACTAATTGCACATACATCCAAATGTTTTTAGAAATAAAAAACCTAATATACACCGATTAGTTTGCAATAAATCCAAGTTTTTTAACCTATATTTCCTAAAACTAATCACTGTCAACTGACATATAAAATATACGTCAGGTACGTAATTAAATATACATATATATATCTTTTATGCAAGCTTTGGAAGTGAGGTTGAAAAACATTATTTATTTGTAATTTTCAGTACATAAACATCATATATGTTGATGTTAACATTACAAAGAATGTTTGAATGGAAGGGTTGAATTGTTGAATAAATGCATTGAAAATGCAGGGTGTAAGAACTGTATGCTTTCAACATGTCACTTTCGCTGTGAACTCTTTAGGCCACATGTGGGGAAACCAACCATGGAACACTGGAGATCAATCTAGAAATAATTGGTGACGTTCACACGTTCCTTTCATTTTTACATTACAGTTCTTTTAAGATTTTGTCTAGTCATTTTTCTCGGATATTGGATTTGTTCTTGTAACGTTTCGGTTCTAGAGACGTATATAAATGAACCTAGATCACACACGATGAAATGGATATTATAGTATATTATAATCTAAAAATCTATAACGAGTGTATTTGATTTGAAAGAAAACAAGAAAAAAAAGTATATAATTGATTTTTATATTTCCAACTATGCTTTGGCTAAACTAGGATGACTTTTGACCATCTTTAATTTTTAAGACTTTGCCACCAGTTGGACTGATATAGAATTTGTTTGGATTTTAGGGTGTTGGCATTACTCATCTTTGGAGAAGGATGGCACAACAACCACCATGCATTTGAGTATTCAGCTCGACATGGCCTAGAATGGTGGCAGTTGGATGTAACTTGGTGGGTTATTTTGTTTCTTCAAGCCATAGGGTTAGCCACTGATTTGAAGTTACCCTCTCAACATCACATGCACAAGTTGGCCATCCAACCCAAAAGCGATTAATGATACTAAAACACTATTTAAAATGACTTTTTAAGGAATTAAAAATTAATTATTTTAAACCCTTAAAGAGTCGTCTCAAACAAAACGTTTCAACTCTCTCTTGATTATTGCTCAAACTTTAAATTTGTAGTTTGGTGTACGAATCATGCCCC

mRNA sequence

ATGGAGGCCACAAGGAAAGAGCTGGAAGATAAAAATAGCGATCAGAAGGCTATGGCGGTTGAAGACAAACCTCGGGCTAGATCCCGGCGAAGACCATTTTTCCGAAGAAAATGGACAACTTTAGACAAACGTTCTGCTGGTGCTTTTCTTGTTATGCATTTTCTCTGTGTTTTGGCACCATTTTATTTCACATGGCCAGCCTTCTGGCTTGCCTTTGTACTCTACATTATCACGGGCTTGTTCGGTTTGACACTCTCGTACCACCGGCATCTTACACACAAGAGTTTCAAACTTCCCAAATGGCTTGAGTACACGTTTGCTTACATTGGAGTCCATACACTTCAAGGCGATCCGATTGATTGGGTGAGTACGCATAGATATCACCATCAGTTTGTGGATACAAAAAGAGATCCACACACTCCGACACAAGGGTTTTTATTTGGACATATTGCTTGGATATTTGATTCATATGGTTTGACTAAAAAAGTTAGCCCTAAACATGTTGAAGATTTCGAAGCAAAAGATGAGAAAAGAAAGATTAGTAGAAATATGTTTGTGGGATATTTGAAGTATGGAAGACCTAACAATGCTGAGGATTTACAAAATCAAGGCTTTTACAGGTTTCTTCGAAGAACATACATTCTTCATCATTTTGGTCTTGCAATAATCCTCTATGCTCTTGGAGGGCTTCCTTTTCTCACTTGGGGAATGGGTGTAAGAACTGTATGCTTTCAACATGTCACTTTCGCTGTGAACTCTTTAGGCCACATGTGGGGAAACCAACCATGGAACACTGGAGATCAATCTAGAAATAATTGGGTGTTGGCATTACTCATCTTTGGAGAAGGATGGCACAACAACCACCATGCATTTGAGTATTCAGCTCGACATGGCCTAGAATGGTGGCAGTTGGATGTAACTTGGTGGGTTATTTTGTTTCTTCAAGCCATAGGGTTAGCCACTGATTTGAAGTTACCCTCTCAACATCACATGCACAAGTTGGCCATCCAACCCAAAAGCGATTAA

Coding sequence (CDS)

ATGGAGGCCACAAGGAAAGAGCTGGAAGATAAAAATAGCGATCAGAAGGCTATGGCGGTTGAAGACAAACCTCGGGCTAGATCCCGGCGAAGACCATTTTTCCGAAGAAAATGGACAACTTTAGACAAACGTTCTGCTGGTGCTTTTCTTGTTATGCATTTTCTCTGTGTTTTGGCACCATTTTATTTCACATGGCCAGCCTTCTGGCTTGCCTTTGTACTCTACATTATCACGGGCTTGTTCGGTTTGACACTCTCGTACCACCGGCATCTTACACACAAGAGTTTCAAACTTCCCAAATGGCTTGAGTACACGTTTGCTTACATTGGAGTCCATACACTTCAAGGCGATCCGATTGATTGGGTGAGTACGCATAGATATCACCATCAGTTTGTGGATACAAAAAGAGATCCACACACTCCGACACAAGGGTTTTTATTTGGACATATTGCTTGGATATTTGATTCATATGGTTTGACTAAAAAAGTTAGCCCTAAACATGTTGAAGATTTCGAAGCAAAAGATGAGAAAAGAAAGATTAGTAGAAATATGTTTGTGGGATATTTGAAGTATGGAAGACCTAACAATGCTGAGGATTTACAAAATCAAGGCTTTTACAGGTTTCTTCGAAGAACATACATTCTTCATCATTTTGGTCTTGCAATAATCCTCTATGCTCTTGGAGGGCTTCCTTTTCTCACTTGGGGAATGGGTGTAAGAACTGTATGCTTTCAACATGTCACTTTCGCTGTGAACTCTTTAGGCCACATGTGGGGAAACCAACCATGGAACACTGGAGATCAATCTAGAAATAATTGGGTGTTGGCATTACTCATCTTTGGAGAAGGATGGCACAACAACCACCATGCATTTGAGTATTCAGCTCGACATGGCCTAGAATGGTGGCAGTTGGATGTAACTTGGTGGGTTATTTTGTTTCTTCAAGCCATAGGGTTAGCCACTGATTTGAAGTTACCCTCTCAACATCACATGCACAAGTTGGCCATCCAACCCAAAAGCGATTAA
BLAST of CSPI04G01710.1 vs. Swiss-Prot
Match: ADS3_ARATH (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana GN=ADS3 PE=1 SV=2)

HSP 1 Score: 373.6 bits (958), Expect = 2.3e-102
Identity = 174/322 (54.04%), Postives = 218/322 (67.70%), Query Frame = 1

Query: 14  DQKAMAVEDKPRARSRRRPFFRRKWTTLDKRSAGAFLVMHFLCVLAPFYFTWPAFWLAFV 73
           D + + + D    +  +  ++ R+W  +D  +    L MH L +LAPF F W A  +AF 
Sbjct: 74  DYRRIMLSDVLVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFG 133

Query: 74  LYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPIDWVSTHRYHHQFVD 133
           LYI+TGL G+TLS+HR+L+HK+FKLPKWLEY FAY G   LQG+PIDWVSTHRYHHQF D
Sbjct: 134 LYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCD 193

Query: 134 TKRDPHTPTQGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKRKISRNMFVGYLKYGR 193
           + RDPH+P  GF F H+ W+FD+  +T++                             G 
Sbjct: 194 SDRDPHSPLDGFWFSHMNWMFDTNTITQRC----------------------------GE 253

Query: 194 PNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMGVRTVCFQHVTFAVNS 253
           PNN  DL+ Q FYRFLR TYILH   LA+ LYA+GG PF+ WGMGVR V   H+T+ VNS
Sbjct: 254 PNNVGDLEKQPFYRFLRTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNS 313

Query: 254 LGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLEWWQLDVTWWVILF 313
             H+WG Q WNTGD S+NNW +A L FGEGWHNNHHAFE+SARHGLEWWQLD+TW+V+ F
Sbjct: 314 ACHVWGKQAWNTGDLSKNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKF 367

Query: 314 LQAIGLATDLKLPSQHHMHKLA 336
           LQAIGLATD+KLPS+    ++A
Sbjct: 374 LQAIGLATDVKLPSEAQKQRMA 367

BLAST of CSPI04G01710.1 vs. Swiss-Prot
Match: ADS1_ARATH (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana GN=ADS1 PE=2 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 5.1e-94
Identity = 165/329 (50.15%), Postives = 214/329 (65.05%), Query Frame = 1

Query: 10  DKNSDQKAMAVEDKPRARSRRRPFFRRKWTTLDKRSAGAFLVMHFLCVLAPFYFTWPAFW 69
           +K  + K MA  DK     ++R  + RKW  LD   A A L +HFLC+LAPF FTWPA  
Sbjct: 7   EKEENNKKMAA-DKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALR 66

Query: 70  LAFVLYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPIDWVSTHRYHH 129
           +A ++Y + GL G+T+SYHR+L H+SFK+PKWLEY FAY G+  +QGDPIDWVSTHRYHH
Sbjct: 67  VALIVYTVGGL-GITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHH 126

Query: 130 QFVDTKRDPHTPTQGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKRKISRNMFVGYL 189
           QF D+ RDPH+P +GF F H+ W+FD+                              GYL
Sbjct: 127 QFTDSDRDPHSPNEGFWFSHLLWLFDT------------------------------GYL 186

Query: 190 --KYGRPNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMGVRTVCFQHV 249
             K GR  N EDL+ Q +Y+FL+RT + H      +LY  GGL FLTWGMG+      HV
Sbjct: 187 VEKCGRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHV 246

Query: 250 TFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLEWWQLDVT 309
           T  +NSL H+WG++ W T D SRN W L++  FGE WHNNHHAFE SAR GLEWWQ+D++
Sbjct: 247 TCLINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDIS 303

Query: 310 WWVILFLQAIGLATDLKLPSQHHMHKLAI 337
           W+++ FL+ IGLATD+KLPS+    ++A+
Sbjct: 307 WYIVRFLEIIGLATDVKLPSESQRRRMAM 303

BLAST of CSPI04G01710.1 vs. Swiss-Prot
Match: ICO5D_LIMDO (Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii PE=1 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.5e-93
Identity = 163/307 (53.09%), Postives = 207/307 (67.43%), Query Frame = 1

Query: 29  RRRPFFRRKWTTLDKRSAGAFLVMHFLCVLAPFYFTWPAFWLAFVLY-IITGLFGLTLSY 88
           + + F+RRKW   D ++A   LV+H L  +APFYF+W AFW++F+L    +G+ G+TL +
Sbjct: 72  KAKGFWRRKWNPRDIQNAVTLLVLHALAAMAPFYFSWDAFWISFILLGFASGVLGITLCF 131

Query: 89  HRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPIDWVSTHRYHHQFVDTKRDPHTPTQGFLF 148
           HR LTH  FKLPK +EY FAY G   LQGDP++WVS HRYHHQFVDT+RD H+PTQGF F
Sbjct: 132 HRCLTHGGFKLPKLVEYFFAYCGSLALQGDPMEWVSNHRYHHQFVDTERDVHSPTQGFWF 191

Query: 149 GHIAWIFDSYGLTKKVSPKHVEDFEAKDEKRKISRNMFVGYLKYGRPNNAEDLQNQGFYR 208
            HI W+ D                          +++FV   + GR NN  DL+ Q FYR
Sbjct: 192 CHIGWVLD--------------------------KDLFV-EKRGGRRNNVNDLKKQAFYR 251

Query: 209 FLRRTYILHHFGLAIILYALGGLPFLTWGMGVRTVCFQHVTFAVNSLGHMWGNQPWNTGD 268
           FL++TY+ H   L  +LY +GG P++ WGMG R V   H TFA+NS+ H WG +PWNTGD
Sbjct: 252 FLQKTYMYHQLALIALLYYVGGFPYIVWGMGFRLVFMFHSTFAINSVCHKWGGRPWNTGD 311

Query: 269 QSRNNWVLALLIFGEGWHNNHHAFEYSARHGLEWWQLDVTWWVILFLQAIGLATDLKLPS 328
            S NN  +AL  FGEGWHNNHHAFE SARHGLEWW++DVTW+VI  LQAIGLAT++KLP+
Sbjct: 312 LSTNNMFVALCAFGEGWHNNHHAFEQSARHGLEWWEIDVTWYVIRTLQAIGLATNVKLPT 351

Query: 329 QHHMHKL 335
           +    KL
Sbjct: 372 EAQKQKL 351

BLAST of CSPI04G01710.1 vs. Swiss-Prot
Match: ADS2_ARATH (Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana GN=ADS2 PE=2 SV=2)

HSP 1 Score: 336.7 bits (862), Expect = 3.1e-91
Identity = 164/334 (49.10%), Postives = 212/334 (63.47%), Query Frame = 1

Query: 3   ATRKELEDKNSDQKAMAVEDKPRARSRRRPFFRRKWTTLDKRSAGAFLVMHFLCVLAPFY 62
           +T +E   KN    A AVE+K   + RR  F+ R+W  LD     A   +H L +LAPFY
Sbjct: 5   STVEENHQKNPSTPA-AVEEK---KKRRWVFWDRRWRRLDYVKFSASFTVHSLALLAPFY 64

Query: 63  FTWPAFWLAFVLYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPIDWV 122
           FTW A W+ F+ Y I GL G+T+SYHR+L H+SFK+PKWLEY  AY  +  +QGDPIDWV
Sbjct: 65  FTWSALWVTFLFYTIGGL-GITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWV 124

Query: 123 STHRYHHQFVDTKRDPHTPTQGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKRKISR 182
           STHRYHHQF D++RDPH+P +GF F H+ WI+DS  L  K                    
Sbjct: 125 STHRYHHQFTDSERDPHSPKEGFWFSHLLWIYDSAYLVSKC------------------- 184

Query: 183 NMFVGYLKYGRPNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMGVRTV 242
                    GR  N EDL+ Q FYRFL++T + H  GL   L+ LGG+ F+TWGMGV   
Sbjct: 185 ---------GRRANVEDLKRQWFYRFLQKTVLFHILGLGFFLFYLGGMSFVTWGMGVGAA 244

Query: 243 CFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLEWW 302
              HVT  +NSL H+WG + W T D SRN W L++  FGE WHNNHHAFE SAR GLEWW
Sbjct: 245 LEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWW 304

Query: 303 QLDVTWWVILFLQAIGLATDLKLPSQHHMHKLAI 337
           Q+D++W+++ F + IGLATD+K+P++    ++AI
Sbjct: 305 QIDISWYIVRFFEIIGLATDVKVPTEAQRRRMAI 305

BLAST of CSPI04G01710.1 vs. Swiss-Prot
Match: ADS32_ARATH (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 331.3 bits (848), Expect = 1.3e-89
Identity = 155/307 (50.49%), Postives = 200/307 (65.15%), Query Frame = 1

Query: 30  RRPFFRRKWTTLDKRSAGAFLVMHFLCVLAPFYFTWPAFWLAFVLYIITGLFGLTLSYHR 89
           +R F+ R WT+ D      F+  H L +LAPFYF+W AFW+   L  I G+  +TLSYHR
Sbjct: 83  KRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITLSYHR 142

Query: 90  HLTHKSFKLPKWLEYTFAYIGVHTLQGDPIDWVSTHRYHHQFVDTKRDPHTPTQGFLFGH 149
           +L+H+SF LPKWLEY FAY GV   QGDPI+WVS HRYHH+  +T+RDPH+PTQGF F H
Sbjct: 143 NLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSH 202

Query: 150 IAWIFDSYGLTKKVSPKHVEDFEAKDEKRKISRNMFVGYLKYGRPNNAEDLQNQGFYRFL 209
           +AWIFD+  + +                              G   N +DL  Q FYRFL
Sbjct: 203 MAWIFDTSSILENC----------------------------GGEENVDDLVRQPFYRFL 262

Query: 210 RRTYILHHFGLAIILYALGGLPFLTWGMGVRTVCFQHVTFAVNSLGHMWGNQPWNTGDQS 269
           +RT +LH    + + Y  GG+P L WG+G+      H+TF VNS+ H+WG + WNT D S
Sbjct: 263 QRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFS 322

Query: 270 RNNWVLALLIFGEGWHNNHHAFEYSARHGLEWWQLDVTWWVILFLQAIGLATDLKLPSQH 329
           +NNW +A+L  GEGWHNNHHAFE+SARHGLEWWQLD+TW +I FL+AIGLAT++KLP++ 
Sbjct: 323 KNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTET 360

Query: 330 HMHKLAI 337
            M   A+
Sbjct: 383 QMKGKAL 360

BLAST of CSPI04G01710.1 vs. TrEMBL
Match: A0A0A0KTM4_CUCSA (Delta-9 fatty acid desaturase OS=Cucumis sativus GN=Csa_4G006150 PE=3 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 2.2e-205
Identity = 338/341 (99.12%), Postives = 339/341 (99.41%), Query Frame = 1

Query: 1   MEATRKELEDKNSDQKAMAVEDKPRARSRRRPFFRRKWTTLDKRSAGAFLVMHFLCVLAP 60
           MEATRKELEDKNSDQKAMAVEDKPR RSRRRPFFRRKWTTLDKRSAGAFLVMHFLCV AP
Sbjct: 1   MEATRKELEDKNSDQKAMAVEDKPRVRSRRRPFFRRKWTTLDKRSAGAFLVMHFLCVFAP 60

Query: 61  FYFTWPAFWLAFVLYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPID 120
           FYFTWPAFWLAFVLY+ITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPID
Sbjct: 61  FYFTWPAFWLAFVLYVITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPID 120

Query: 121 WVSTHRYHHQFVDTKRDPHTPTQGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKRKI 180
           WVSTHRYHHQFVDTKRDPHTPTQGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKRKI
Sbjct: 121 WVSTHRYHHQFVDTKRDPHTPTQGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKRKI 180

Query: 181 SRNMFVGYLKYGRPNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMGVR 240
           SRNMFVGYLKYGRPNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMGVR
Sbjct: 181 SRNMFVGYLKYGRPNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMGVR 240

Query: 241 TVCFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLE 300
           TVCFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLE
Sbjct: 241 TVCFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLE 300

Query: 301 WWQLDVTWWVILFLQAIGLATDLKLPSQHHMHKLAIQPKSD 342
           WWQLDVTWWVILFLQAIGLATDLKLPSQHHMHKLAIQPKSD
Sbjct: 301 WWQLDVTWWVILFLQAIGLATDLKLPSQHHMHKLAIQPKSD 341

BLAST of CSPI04G01710.1 vs. TrEMBL
Match: A0A0A0KVI7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006160 PE=4 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 3.2e-164
Identity = 269/341 (78.89%), Postives = 300/341 (87.98%), Query Frame = 1

Query: 1   MEATRKELEDKNSDQKAMAVEDKPRARSRRR-PFFRRKWTTLDKRSAGAFLVMHFLCVLA 60
           MEATRKELEDKN+D KAM VE K R R RRR P FRRKWT LD+RS G  + MH L +LA
Sbjct: 7   MEATRKELEDKNNDHKAMTVEGKARVRPRRRRPLFRRKWTLLDRRSFGGVMAMHLLSLLA 66

Query: 61  PFYFTWPAFWLAFVLYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPI 120
           PFYFTWPAFWLA VLYI+TGLFG+TLSYHR L+HKSFKLPKWLEYTFAY+GVH LQGDPI
Sbjct: 67  PFYFTWPAFWLAVVLYILTGLFGVTLSYHRQLSHKSFKLPKWLEYTFAYMGVHALQGDPI 126

Query: 121 DWVSTHRYHHQFVDTKRDPHTPT-QGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKR 180
           DW STHR HHQFVDT+RDPH+P+ +GF FGHIAWIFDSYGLT+KV+PK+VE F+ +DEKR
Sbjct: 127 DWASTHRIHHQFVDTERDPHSPSVEGFFFGHIAWIFDSYGLTEKVNPKYVESFKERDEKR 186

Query: 181 KISRNMFVGYLKYGRPNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMG 240
           K   N+FVGYLKYGRP N EDLQ Q FYRF+R TYILHHFGLAIILY +GGLPFL WGMG
Sbjct: 187 KTFSNIFVGYLKYGRPKNVEDLQKQAFYRFIRSTYILHHFGLAIILYVVGGLPFLVWGMG 246

Query: 241 VRTVCFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHG 300
           VR VCFQHVTF +NS GH+WG+Q WNTGDQSRNNW++AL +FGEGWHNNHHAFEYSARHG
Sbjct: 247 VRIVCFQHVTFFLNSAGHLWGSQQWNTGDQSRNNWMVALFMFGEGWHNNHHAFEYSARHG 306

Query: 301 LEWWQLDVTWWVILFLQAIGLATDLKLPSQHHMHKLAIQPK 340
           LEWWQ DVTWW+ILFL+AIGLATD+KLPS++HM KLAIQPK
Sbjct: 307 LEWWQFDVTWWIILFLEAIGLATDVKLPSRNHMQKLAIQPK 347

BLAST of CSPI04G01710.1 vs. TrEMBL
Match: A0A0A0KWV4_CUCSA (Delta 9 desaturase OS=Cucumis sativus GN=Csa_4G006140 PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 2.6e-137
Identity = 222/339 (65.49%), Postives = 267/339 (78.76%), Query Frame = 1

Query: 1   MEATRKELEDKNSDQKAMAVEDKPRARSRRRPFFRRKWTTLDKRSAGAFLVMHFLCVLAP 60
           MEA RKE+EDK+  +K  ++ +  RA      FF RKWTTLD+R+A   L +H L +LAP
Sbjct: 1   MEAARKEVEDKHDKKKQFSMVNPKRA------FFNRKWTTLDRRTAAGILFLHLLSLLAP 60

Query: 61  FYFTWPAFWLAFVLYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPID 120
           F+F W AFW+AF LY+ITG  G+TLSYHR+L H+SFKLPKWLEY FAY+GV   QGDPID
Sbjct: 61  FHFNWAAFWVAFALYLITGALGITLSYHRNLAHRSFKLPKWLEYFFAYLGVQGFQGDPID 120

Query: 121 WVSTHRYHHQFVDTKRDPHTPTQGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKRKI 180
           WVSTHRYHHQFVDT RDPH+P QGF F H+ W+FDSYGLT+K+ PK++EDF+ ++ KR +
Sbjct: 121 WVSTHRYHHQFVDTDRDPHSPMQGFWFSHMTWMFDSYGLTEKIYPKNLEDFKEREAKRTL 180

Query: 181 SRNMFVGYLKYGRPNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMGVR 240
           SRN FVG+LKYGRP N EDLQ QGFYRFLR+TY+LH   LAI+LY  GGLPF+ WGMGVR
Sbjct: 181 SRNWFVGFLKYGRPKNVEDLQKQGFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVR 240

Query: 241 TVCFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLE 300
            +C  H    VNS  H+WG + WNT D S+NNW++ L+ FGEGWHNNHHAFEYSARHGL+
Sbjct: 241 LICVLHAALFVNSACHIWGYRKWNTNDLSKNNWIIGLISFGEGWHNNHHAFEYSARHGLQ 300

Query: 301 WWQLDVTWWVILFLQAIGLATDLKLPSQHHMHKLAIQPK 340
           WW+ D TWWVIL LQA+GLATD+KLP+Q H  KLAIQ K
Sbjct: 301 WWEFDFTWWVILLLQALGLATDVKLPTQLHKQKLAIQYK 333

BLAST of CSPI04G01710.1 vs. TrEMBL
Match: A0A0A0KVH2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006060 PE=3 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 2.1e-115
Identity = 199/341 (58.36%), Postives = 242/341 (70.97%), Query Frame = 1

Query: 3   ATRKELEDKNSDQKAMAVEDKPRARSRR----RPFFRRKWTTLDKRSAGAFLVMHFLCVL 62
           A  KE++     +  +  E K     +     R F+RR WTTLDKR A   L +H LC+ 
Sbjct: 2   AAEKEVKPHEGHKPLVMDESKQEEPKKMVKPPRSFWRRNWTTLDKRVAYIILFVHLLCIF 61

Query: 63  APFYFTWPAFWLAFVLYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDP 122
           APF F W AF +   L +ITGLFG+T+SYHR+L HKSF+LPKWLEY+FAY GVH LQGDP
Sbjct: 62  APFQFNWSAFRVTVALIVITGLFGMTISYHRNLAHKSFQLPKWLEYSFAYCGVHCLQGDP 121

Query: 123 IDWVSTHRYHHQFVDTKRDPHTPTQGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKR 182
           +DWVSTHR HHQFVD+++DPH+P  GF   H+ W+FDSY LT KV PK+  D       +
Sbjct: 122 LDWVSTHRCHHQFVDSEKDPHSPINGFWISHMMWLFDSYTLTNKVCPKYSSDL------K 181

Query: 183 KISRNMFVGYLKYGRPNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMG 242
           KI RNMFV + K+G+P+N  DL+ Q FYRF+ +TY+LHH  LAIILY++GG+PF  WGM 
Sbjct: 182 KIERNMFVIFTKHGKPDNVGDLEKQRFYRFIHKTYMLHHLALAIILYSIGGVPFFIWGMC 241

Query: 243 VRTVCFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHG 302
           VR V   H TF VNS+ H+WG QPW TGD SRNNW++ L  FGEGWHNNHHAF YSAR G
Sbjct: 242 VRIVAVFHSTFMVNSVCHIWGKQPWRTGDLSRNNWLVGLTSFGEGWHNNHHAFSYSARLG 301

Query: 303 LEWWQLDVTWWVILFLQAIGLATDLKLPSQHHMHKLAIQPK 340
           LEWWQLD  W+VI FLQ IGLAT++KLPS  HM  LA+  K
Sbjct: 302 LEWWQLDAGWYVIKFLQVIGLATNVKLPSPTHMQSLAMDHK 336

BLAST of CSPI04G01710.1 vs. TrEMBL
Match: A0A0A0LTL5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G227450 PE=3 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 6.8e-114
Identity = 190/302 (62.91%), Postives = 230/302 (76.16%), Query Frame = 1

Query: 33  FFRRKWTTLDKRSAGAFLVMHFLCVLAPFYFTWPAFWLAFVLYIITGLFGLTLSYHRHLT 92
           F +RKWT  DK  A  F+ +HF+C+LAPF+F W AFW+AF LYIITG FG+T+SYHR+L+
Sbjct: 13  FGQRKWTNRDKYMAAFFIFLHFICILAPFHFNWNAFWVAFALYIITGCFGITISYHRNLS 72

Query: 93  HKSFKLPKWLEYTFAYIGVHTLQGDPIDWVSTHRYHHQFVDTKRDPHTPTQGFLFGHIAW 152
           H+SF+LPKWLEY FAY G    QGDPIDWVSTHR HHQFVDT+ DPH+P QGF F +  W
Sbjct: 73  HRSFRLPKWLEYFFAYCGALAFQGDPIDWVSTHRCHHQFVDTENDPHSPIQGFWFSYFIW 132

Query: 153 IFDSYGLTKKVSPKHVEDFEAKDEKRKISRNMFVGYLKYGRPNNAEDLQNQGFYRFLRRT 212
           I DS  LT++V P++  D   KD ++ I    F   LKYGRPNN  DL+ Q FYRFLR+T
Sbjct: 133 ILDSNALTRRVCPQYFIDH--KDTEKTI----FTVVLKYGRPNNVGDLEKQFFYRFLRKT 192

Query: 213 YILHHFGLAIILYALGGLPFLTWGMGVRTVCFQHVTFAVNSLGHMWGNQPWNTGDQSRNN 272
           Y LH   LAI+LYA+GG PFL WG  VRT+   HVTF VNS+ H +GNQPWNTGD SRN 
Sbjct: 193 YFLHQLALAILLYAVGGTPFLIWGAFVRTIGTIHVTFMVNSICHTFGNQPWNTGDLSRNT 252

Query: 273 WVLALLIFGEGWHNNHHAFEYSARHGLEWWQLDVTWWVILFLQAIGLATDLKLPSQHHMH 332
           W + L+ FGEGWHNNHHAFEYSAR GLEWWQ+D++W++I FLQ IGLAT++K+PSQ H  
Sbjct: 253 WWMCLISFGEGWHNNHHAFEYSARQGLEWWQIDISWYIIWFLQVIGLATEVKVPSQSHKQ 308

Query: 333 KL 335
           +L
Sbjct: 313 RL 308

BLAST of CSPI04G01710.1 vs. TAIR10
Match: AT3G15850.1 (AT3G15850.1 fatty acid desaturase 5)

HSP 1 Score: 373.6 bits (958), Expect = 1.3e-103
Identity = 174/322 (54.04%), Postives = 218/322 (67.70%), Query Frame = 1

Query: 14  DQKAMAVEDKPRARSRRRPFFRRKWTTLDKRSAGAFLVMHFLCVLAPFYFTWPAFWLAFV 73
           D + + + D    +  +  ++ R+W  +D  +    L MH L +LAPF F W A  +AF 
Sbjct: 74  DYRRIMLSDVLVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFG 133

Query: 74  LYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPIDWVSTHRYHHQFVD 133
           LYI+TGL G+TLS+HR+L+HK+FKLPKWLEY FAY G   LQG+PIDWVSTHRYHHQF D
Sbjct: 134 LYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCD 193

Query: 134 TKRDPHTPTQGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKRKISRNMFVGYLKYGR 193
           + RDPH+P  GF F H+ W+FD+  +T++                             G 
Sbjct: 194 SDRDPHSPLDGFWFSHMNWMFDTNTITQRC----------------------------GE 253

Query: 194 PNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMGVRTVCFQHVTFAVNS 253
           PNN  DL+ Q FYRFLR TYILH   LA+ LYA+GG PF+ WGMGVR V   H+T+ VNS
Sbjct: 254 PNNVGDLEKQPFYRFLRTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNS 313

Query: 254 LGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLEWWQLDVTWWVILF 313
             H+WG Q WNTGD S+NNW +A L FGEGWHNNHHAFE+SARHGLEWWQLD+TW+V+ F
Sbjct: 314 ACHVWGKQAWNTGDLSKNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKF 367

Query: 314 LQAIGLATDLKLPSQHHMHKLA 336
           LQAIGLATD+KLPS+    ++A
Sbjct: 374 LQAIGLATDVKLPSEAQKQRMA 367

BLAST of CSPI04G01710.1 vs. TAIR10
Match: AT1G06080.1 (AT1G06080.1 delta 9 desaturase 1)

HSP 1 Score: 345.9 bits (886), Expect = 2.8e-95
Identity = 165/329 (50.15%), Postives = 214/329 (65.05%), Query Frame = 1

Query: 10  DKNSDQKAMAVEDKPRARSRRRPFFRRKWTTLDKRSAGAFLVMHFLCVLAPFYFTWPAFW 69
           +K  + K MA  DK     ++R  + RKW  LD   A A L +HFLC+LAPF FTWPA  
Sbjct: 7   EKEENNKKMAA-DKAEMGRKKRAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALR 66

Query: 70  LAFVLYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPIDWVSTHRYHH 129
           +A ++Y + GL G+T+SYHR+L H+SFK+PKWLEY FAY G+  +QGDPIDWVSTHRYHH
Sbjct: 67  VALIVYTVGGL-GITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHH 126

Query: 130 QFVDTKRDPHTPTQGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKRKISRNMFVGYL 189
           QF D+ RDPH+P +GF F H+ W+FD+                              GYL
Sbjct: 127 QFTDSDRDPHSPNEGFWFSHLLWLFDT------------------------------GYL 186

Query: 190 --KYGRPNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMGVRTVCFQHV 249
             K GR  N EDL+ Q +Y+FL+RT + H      +LY  GGL FLTWGMG+      HV
Sbjct: 187 VEKCGRRTNVEDLKRQWYYKFLQRTVLYHILTFGFLLYYFGGLSFLTWGMGIGVAMEHHV 246

Query: 250 TFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLEWWQLDVT 309
           T  +NSL H+WG++ W T D SRN W L++  FGE WHNNHHAFE SAR GLEWWQ+D++
Sbjct: 247 TCLINSLCHVWGSRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWWQIDIS 303

Query: 310 WWVILFLQAIGLATDLKLPSQHHMHKLAI 337
           W+++ FL+ IGLATD+KLPS+    ++A+
Sbjct: 307 WYIVRFLEIIGLATDVKLPSESQRRRMAM 303

BLAST of CSPI04G01710.1 vs. TAIR10
Match: AT2G31360.1 (AT2G31360.1 16:0delta9 desaturase 2)

HSP 1 Score: 336.7 bits (862), Expect = 1.7e-92
Identity = 164/334 (49.10%), Postives = 212/334 (63.47%), Query Frame = 1

Query: 3   ATRKELEDKNSDQKAMAVEDKPRARSRRRPFFRRKWTTLDKRSAGAFLVMHFLCVLAPFY 62
           +T +E   KN    A AVE+K   + RR  F+ R+W  LD     A   +H L +LAPFY
Sbjct: 5   STVEENHQKNPSTPA-AVEEK---KKRRWVFWDRRWRRLDYVKFSASFTVHSLALLAPFY 64

Query: 63  FTWPAFWLAFVLYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPIDWV 122
           FTW A W+ F+ Y I GL G+T+SYHR+L H+SFK+PKWLEY  AY  +  +QGDPIDWV
Sbjct: 65  FTWSALWVTFLFYTIGGL-GITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPIDWV 124

Query: 123 STHRYHHQFVDTKRDPHTPTQGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKRKISR 182
           STHRYHHQF D++RDPH+P +GF F H+ WI+DS  L  K                    
Sbjct: 125 STHRYHHQFTDSERDPHSPKEGFWFSHLLWIYDSAYLVSKC------------------- 184

Query: 183 NMFVGYLKYGRPNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMGVRTV 242
                    GR  N EDL+ Q FYRFL++T + H  GL   L+ LGG+ F+TWGMGV   
Sbjct: 185 ---------GRRANVEDLKRQWFYRFLQKTVLFHILGLGFFLFYLGGMSFVTWGMGVGAA 244

Query: 243 CFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLEWW 302
              HVT  +NSL H+WG + W T D SRN W L++  FGE WHNNHHAFE SAR GLEWW
Sbjct: 245 LEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVFSFGESWHNNHHAFESSARQGLEWW 304

Query: 303 QLDVTWWVILFLQAIGLATDLKLPSQHHMHKLAI 337
           Q+D++W+++ F + IGLATD+K+P++    ++AI
Sbjct: 305 QIDISWYIVRFFEIIGLATDVKVPTEAQRRRMAI 305

BLAST of CSPI04G01710.1 vs. TAIR10
Match: AT3G15870.1 (AT3G15870.1 Fatty acid desaturase family protein)

HSP 1 Score: 331.3 bits (848), Expect = 7.3e-91
Identity = 155/307 (50.49%), Postives = 200/307 (65.15%), Query Frame = 1

Query: 30  RRPFFRRKWTTLDKRSAGAFLVMHFLCVLAPFYFTWPAFWLAFVLYIITGLFGLTLSYHR 89
           +R F+ R WT+ D      F+  H L +LAPFYF+W AFW+   L  I G+  +TLSYHR
Sbjct: 83  KRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGIC-ITLSYHR 142

Query: 90  HLTHKSFKLPKWLEYTFAYIGVHTLQGDPIDWVSTHRYHHQFVDTKRDPHTPTQGFLFGH 149
           +L+H+SF LPKWLEY FAY GV   QGDPI+WVS HRYHH+  +T+RDPH+PTQGF F H
Sbjct: 143 NLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQGFWFSH 202

Query: 150 IAWIFDSYGLTKKVSPKHVEDFEAKDEKRKISRNMFVGYLKYGRPNNAEDLQNQGFYRFL 209
           +AWIFD+  + +                              G   N +DL  Q FYRFL
Sbjct: 203 MAWIFDTSSILENC----------------------------GGEENVDDLVRQPFYRFL 262

Query: 210 RRTYILHHFGLAIILYALGGLPFLTWGMGVRTVCFQHVTFAVNSLGHMWGNQPWNTGDQS 269
           +RT +LH    + + Y  GG+P L WG+G+      H+TF VNS+ H+WG + WNT D S
Sbjct: 263 QRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFS 322

Query: 270 RNNWVLALLIFGEGWHNNHHAFEYSARHGLEWWQLDVTWWVILFLQAIGLATDLKLPSQH 329
           +NNW +A+L  GEGWHNNHHAFE+SARHGLEWWQLD+TW +I FL+AIGLAT++KLP++ 
Sbjct: 323 KNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTET 360

Query: 330 HMHKLAI 337
            M   A+
Sbjct: 383 QMKGKAL 360

BLAST of CSPI04G01710.1 vs. TAIR10
Match: AT1G06100.1 (AT1G06100.1 Fatty acid desaturase family protein)

HSP 1 Score: 318.5 bits (815), Expect = 4.9e-87
Identity = 156/315 (49.52%), Postives = 197/315 (62.54%), Query Frame = 1

Query: 23  KPRARSRRRPFFRRKWTTLDKRSAGAFLVMHFLCVLAPFYFTWPAFWLAFVLYIITGLFG 82
           K   R  +R +  RKWT  D   A     +H LC+LAPF + W AF    +L I+T L  
Sbjct: 13  KKSVRKEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRFGIILAILTNLC- 72

Query: 83  LTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPIDWVSTHRYHHQFVDTKRDPHTPT 142
           +T SYHR+LTH+SFKLPKWLEY FAY  +  LQGDP+DWVS HR+HHQF D+ RDPH+P 
Sbjct: 73  ITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQFTDSDRDPHSPI 132

Query: 143 QGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKRKISRNMFVGYLKYGRPNNAEDLQN 202
           +GF F H+ WIFD+  + +K                             GR NN  DL+ 
Sbjct: 133 EGFWFSHVLWIFDTDYIREKC----------------------------GRRNNVMDLKQ 192

Query: 203 QGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMGVRTVCFQHVTFAVNSLGHMWGNQP 262
           Q FYRFL++T +LH      ++Y  GGLP+LTW +G   V   H T+ VNS  H+ G+Q 
Sbjct: 193 QWFYRFLKKTLVLHILAFWTLIYLWGGLPYLTWTVGFGGVIGYHGTWLVNSACHICGSQA 252

Query: 263 WNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLEWWQLDVTWWVILFLQAIGLATD 322
           W T D SRN W LALL  GE WHNNHHAFE SARHGLEW+QLD+TW++I F QA+GLAT+
Sbjct: 253 WQTNDTSRNVWWLALLTMGESWHNNHHAFETSARHGLEWYQLDITWYLIWFFQALGLATN 298

Query: 323 LKLPSQHHMHKLAIQ 338
           +KLP+     K+AI+
Sbjct: 313 VKLPTDAQKRKMAIR 298

BLAST of CSPI04G01710.1 vs. NCBI nr
Match: gi|778689584|ref|XP_011652981.1| (PREDICTED: delta-9 acyl-lipid desaturase 1-like [Cucumis sativus])

HSP 1 Score: 722.6 bits (1864), Expect = 3.2e-205
Identity = 338/341 (99.12%), Postives = 339/341 (99.41%), Query Frame = 1

Query: 1   MEATRKELEDKNSDQKAMAVEDKPRARSRRRPFFRRKWTTLDKRSAGAFLVMHFLCVLAP 60
           MEATRKELEDKNSDQKAMAVEDKPR RSRRRPFFRRKWTTLDKRSAGAFLVMHFLCV AP
Sbjct: 1   MEATRKELEDKNSDQKAMAVEDKPRVRSRRRPFFRRKWTTLDKRSAGAFLVMHFLCVFAP 60

Query: 61  FYFTWPAFWLAFVLYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPID 120
           FYFTWPAFWLAFVLY+ITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPID
Sbjct: 61  FYFTWPAFWLAFVLYVITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPID 120

Query: 121 WVSTHRYHHQFVDTKRDPHTPTQGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKRKI 180
           WVSTHRYHHQFVDTKRDPHTPTQGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKRKI
Sbjct: 121 WVSTHRYHHQFVDTKRDPHTPTQGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKRKI 180

Query: 181 SRNMFVGYLKYGRPNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMGVR 240
           SRNMFVGYLKYGRPNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMGVR
Sbjct: 181 SRNMFVGYLKYGRPNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMGVR 240

Query: 241 TVCFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLE 300
           TVCFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLE
Sbjct: 241 TVCFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLE 300

Query: 301 WWQLDVTWWVILFLQAIGLATDLKLPSQHHMHKLAIQPKSD 342
           WWQLDVTWWVILFLQAIGLATDLKLPSQHHMHKLAIQPKSD
Sbjct: 301 WWQLDVTWWVILFLQAIGLATDLKLPSQHHMHKLAIQPKSD 341

BLAST of CSPI04G01710.1 vs. NCBI nr
Match: gi|659108469|ref|XP_008454215.1| (PREDICTED: delta-9 acyl-lipid desaturase 1-like [Cucumis melo])

HSP 1 Score: 690.3 bits (1780), Expect = 1.7e-195
Identity = 321/341 (94.13%), Postives = 329/341 (96.48%), Query Frame = 1

Query: 1   MEATRKELEDKNSDQKAMAVEDKPRARSRRRPFFRRKWTTLDKRSAGAFLVMHFLCVLAP 60
           ME  RKELEDKNSDQKAMAV DK R RSRRRPFFRRKWTTLDKRSAGAFLVMHFLC+LAP
Sbjct: 1   METIRKELEDKNSDQKAMAVGDKARVRSRRRPFFRRKWTTLDKRSAGAFLVMHFLCLLAP 60

Query: 61  FYFTWPAFWLAFVLYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPID 120
           FYFTWPAFWLA  LYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPID
Sbjct: 61  FYFTWPAFWLAVALYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPID 120

Query: 121 WVSTHRYHHQFVDTKRDPHTPTQGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKRKI 180
           WVSTHRYHHQFVDT+RDPHTPTQGFLFGHIAWIFDSYGLTKKV PKHVEDFEAKDEKRK 
Sbjct: 121 WVSTHRYHHQFVDTERDPHTPTQGFLFGHIAWIFDSYGLTKKVCPKHVEDFEAKDEKRKS 180

Query: 181 SRNMFVGYLKYGRPNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMGVR 240
           ++NMFVGYLKYGRPNNAEDLQ Q FYRFLRRTYILHHFGLAIILYA+GGLPFLTWGMGVR
Sbjct: 181 TKNMFVGYLKYGRPNNAEDLQKQAFYRFLRRTYILHHFGLAIILYAVGGLPFLTWGMGVR 240

Query: 241 TVCFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLE 300
           TVCFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLE
Sbjct: 241 TVCFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLE 300

Query: 301 WWQLDVTWWVILFLQAIGLATDLKLPSQHHMHKLAIQPKSD 342
           WWQ DVTWWVILFLQA+GLATDLKLPS+HHMHKLAIQPKS+
Sbjct: 301 WWQFDVTWWVILFLQALGLATDLKLPSKHHMHKLAIQPKSE 341

BLAST of CSPI04G01710.1 vs. NCBI nr
Match: gi|659108467|ref|XP_008454214.1| (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 600.5 bits (1547), Expect = 1.8e-168
Identity = 278/343 (81.05%), Postives = 307/343 (89.50%), Query Frame = 1

Query: 1   MEATRKELEDKNSDQKAMAVEDKPRARSRRR-PFFRRKWTTLDKRSAGAFLVMHFLCVLA 60
           MEATRKELEDKNSD K MAVEDK R R RRR PFFRRKWT LD+RS G  + MH L +LA
Sbjct: 7   MEATRKELEDKNSDHKGMAVEDKARVRPRRRRPFFRRKWTLLDRRSFGGVMAMHLLSLLA 66

Query: 61  PFYFTWPAFWLAFVLYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPI 120
           PFYFTWPAFWLA VLYIITGLFG+TLSYHR L+HKSFKLPKWLEYTFAY+GVH+LQGDPI
Sbjct: 67  PFYFTWPAFWLAVVLYIITGLFGVTLSYHRQLSHKSFKLPKWLEYTFAYMGVHSLQGDPI 126

Query: 121 DWVSTHRYHHQFVDTKRDPHTPT-QGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKR 180
           DWVSTHR HHQFVDT+RDPH+PT  GF FGHIAWIFDSY LT+KV+PK++E FE ++EKR
Sbjct: 127 DWVSTHRIHHQFVDTERDPHSPTVYGFWFGHIAWIFDSYDLTEKVNPKYIEKFEERNEKR 186

Query: 181 KISRNMFVGYLKYGRPNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMG 240
           K   N+FVGYLKYGRP NAEDLQ Q FYRF+RRTYILHHFGLAIILY +GGLPFL WGMG
Sbjct: 187 KTFSNIFVGYLKYGRPKNAEDLQKQAFYRFIRRTYILHHFGLAIILYVVGGLPFLVWGMG 246

Query: 241 VRTVCFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHG 300
           VR VCFQHVTF++NS GHMWG Q WNTGDQSRNNW++ALL+FGEGWHNNHHAFEYSARHG
Sbjct: 247 VRIVCFQHVTFSLNSAGHMWGKQQWNTGDQSRNNWMVALLMFGEGWHNNHHAFEYSARHG 306

Query: 301 LEWWQLDVTWWVILFLQAIGLATDLKLPSQHHMHKLAIQPKSD 342
           LEWWQ DVTWW+ILFL+AIGLATD+KLPS++HM KLAIQPKS+
Sbjct: 307 LEWWQFDVTWWIILFLEAIGLATDVKLPSRNHMQKLAIQPKSE 349

BLAST of CSPI04G01710.1 vs. NCBI nr
Match: gi|778689587|ref|XP_011652982.1| (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 585.9 bits (1509), Expect = 4.6e-164
Identity = 269/341 (78.89%), Postives = 300/341 (87.98%), Query Frame = 1

Query: 1   MEATRKELEDKNSDQKAMAVEDKPRARSRRR-PFFRRKWTTLDKRSAGAFLVMHFLCVLA 60
           MEATRKELEDKN+D KAM VE K R R RRR P FRRKWT LD+RS G  + MH L +LA
Sbjct: 7   MEATRKELEDKNNDHKAMTVEGKARVRPRRRRPLFRRKWTLLDRRSFGGVMAMHLLSLLA 66

Query: 61  PFYFTWPAFWLAFVLYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPI 120
           PFYFTWPAFWLA VLYI+TGLFG+TLSYHR L+HKSFKLPKWLEYTFAY+GVH LQGDPI
Sbjct: 67  PFYFTWPAFWLAVVLYILTGLFGVTLSYHRQLSHKSFKLPKWLEYTFAYMGVHALQGDPI 126

Query: 121 DWVSTHRYHHQFVDTKRDPHTPT-QGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKR 180
           DW STHR HHQFVDT+RDPH+P+ +GF FGHIAWIFDSYGLT+KV+PK+VE F+ +DEKR
Sbjct: 127 DWASTHRIHHQFVDTERDPHSPSVEGFFFGHIAWIFDSYGLTEKVNPKYVESFKERDEKR 186

Query: 181 KISRNMFVGYLKYGRPNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMG 240
           K   N+FVGYLKYGRP N EDLQ Q FYRF+R TYILHHFGLAIILY +GGLPFL WGMG
Sbjct: 187 KTFSNIFVGYLKYGRPKNVEDLQKQAFYRFIRSTYILHHFGLAIILYVVGGLPFLVWGMG 246

Query: 241 VRTVCFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHG 300
           VR VCFQHVTF +NS GH+WG+Q WNTGDQSRNNW++AL +FGEGWHNNHHAFEYSARHG
Sbjct: 247 VRIVCFQHVTFFLNSAGHLWGSQQWNTGDQSRNNWMVALFMFGEGWHNNHHAFEYSARHG 306

Query: 301 LEWWQLDVTWWVILFLQAIGLATDLKLPSQHHMHKLAIQPK 340
           LEWWQ DVTWW+ILFL+AIGLATD+KLPS++HM KLAIQPK
Sbjct: 307 LEWWQFDVTWWIILFLEAIGLATDVKLPSRNHMQKLAIQPK 347

BLAST of CSPI04G01710.1 vs. NCBI nr
Match: gi|778689581|ref|XP_004152341.2| (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 496.5 bits (1277), Expect = 3.7e-137
Identity = 222/339 (65.49%), Postives = 267/339 (78.76%), Query Frame = 1

Query: 1   MEATRKELEDKNSDQKAMAVEDKPRARSRRRPFFRRKWTTLDKRSAGAFLVMHFLCVLAP 60
           MEA RKE+EDK+  +K  ++ +  RA      FF RKWTTLD+R+A   L +H L +LAP
Sbjct: 1   MEAARKEVEDKHDKKKQFSMVNPKRA------FFNRKWTTLDRRTAAGILFLHLLSLLAP 60

Query: 61  FYFTWPAFWLAFVLYIITGLFGLTLSYHRHLTHKSFKLPKWLEYTFAYIGVHTLQGDPID 120
           F+F W AFW+AF LY+ITG  G+TLSYHR+L H+SFKLPKWLEY FAY+GV   QGDPID
Sbjct: 61  FHFNWAAFWVAFALYLITGALGITLSYHRNLAHRSFKLPKWLEYFFAYLGVQGFQGDPID 120

Query: 121 WVSTHRYHHQFVDTKRDPHTPTQGFLFGHIAWIFDSYGLTKKVSPKHVEDFEAKDEKRKI 180
           WVSTHRYHHQFVDT RDPH+P QGF F H+ W+FDSYGLT+K+ PK++EDF+ ++ KR +
Sbjct: 121 WVSTHRYHHQFVDTDRDPHSPMQGFWFSHMTWMFDSYGLTEKIYPKNLEDFKEREAKRTL 180

Query: 181 SRNMFVGYLKYGRPNNAEDLQNQGFYRFLRRTYILHHFGLAIILYALGGLPFLTWGMGVR 240
           SRN FVG+LKYGRP N EDLQ QGFYRFLR+TY+LH   LAI+LY  GGLPF+ WGMGVR
Sbjct: 181 SRNWFVGFLKYGRPKNVEDLQKQGFYRFLRKTYLLHPIALAIVLYKFGGLPFVVWGMGVR 240

Query: 241 TVCFQHVTFAVNSLGHMWGNQPWNTGDQSRNNWVLALLIFGEGWHNNHHAFEYSARHGLE 300
            +C  H    VNS  H+WG + WNT D S+NNW++ L+ FGEGWHNNHHAFEYSARHGL+
Sbjct: 241 LICVLHAALFVNSACHIWGYRKWNTNDLSKNNWIIGLISFGEGWHNNHHAFEYSARHGLQ 300

Query: 301 WWQLDVTWWVILFLQAIGLATDLKLPSQHHMHKLAIQPK 340
           WW+ D TWWVIL LQA+GLATD+KLP+Q H  KLAIQ K
Sbjct: 301 WWEFDFTWWVILLLQALGLATDVKLPTQLHKQKLAIQYK 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ADS3_ARATH2.3e-10254.04Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
ADS1_ARATH5.1e-9450.15Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana GN=ADS1 PE=2 SV=1[more]
ICO5D_LIMDO1.5e-9353.09Icosanoyl-CoA 5-desaturase (Fragment) OS=Limnanthes douglasii PE=1 SV=1[more]
ADS2_ARATH3.1e-9149.10Delta-9 acyl-lipid desaturase 2 OS=Arabidopsis thaliana GN=ADS2 PE=2 SV=2[more]
ADS32_ARATH1.3e-8950.49Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana GN=ADS3.... [more]
Match NameE-valueIdentityDescription
A0A0A0KTM4_CUCSA2.2e-20599.12Delta-9 fatty acid desaturase OS=Cucumis sativus GN=Csa_4G006150 PE=3 SV=1[more]
A0A0A0KVI7_CUCSA3.2e-16478.89Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006160 PE=4 SV=1[more]
A0A0A0KWV4_CUCSA2.6e-13765.49Delta 9 desaturase OS=Cucumis sativus GN=Csa_4G006140 PE=3 SV=1[more]
A0A0A0KVH2_CUCSA2.1e-11558.36Uncharacterized protein OS=Cucumis sativus GN=Csa_4G006060 PE=3 SV=1[more]
A0A0A0LTL5_CUCSA6.8e-11462.91Uncharacterized protein OS=Cucumis sativus GN=Csa_1G227450 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G15850.11.3e-10354.04 fatty acid desaturase 5[more]
AT1G06080.12.8e-9550.15 delta 9 desaturase 1[more]
AT2G31360.11.7e-9249.10 16:0delta9 desaturase 2[more]
AT3G15870.17.3e-9150.49 Fatty acid desaturase family protein[more]
AT1G06100.14.9e-8749.52 Fatty acid desaturase family protein[more]
Match NameE-valueIdentityDescription
gi|778689584|ref|XP_011652981.1|3.2e-20599.12PREDICTED: delta-9 acyl-lipid desaturase 1-like [Cucumis sativus][more]
gi|659108469|ref|XP_008454215.1|1.7e-19594.13PREDICTED: delta-9 acyl-lipid desaturase 1-like [Cucumis melo][more]
gi|659108467|ref|XP_008454214.1|1.8e-16881.05PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
gi|778689587|ref|XP_011652982.1|4.6e-16478.89PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
gi|778689581|ref|XP_004152341.2|3.7e-13765.49PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR005804FA_desaturase_dom
IPR015876Acyl-CoA_DS
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
GO:0055114oxidation-reduction process
Vocabulary: Molecular Function
TermDefinition
GO:0016717oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006633 fatty acid biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI04G01710CSPI04G01710gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI04G01710.1CSPI04G01710.1-proteinpolypeptide


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI04G01710.1.utr3p1CSPI04G01710.1.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI04G01710.1.cds4CSPI04G01710.1.cds4CDS
CSPI04G01710.1.cds3CSPI04G01710.1.cds3CDS
CSPI04G01710.1.cds2CSPI04G01710.1.cds2CDS
CSPI04G01710.1.cds1CSPI04G01710.1.cds1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI04G01710.1.utr5p1CSPI04G01710.1.utr5p1five_prime_UTR


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 68..309
score: 5.5
IPR015876Fatty acid desaturase, type 1, corePRINTSPR00075FACDDSATRASEcoord: 88..108
score: 1.4E-9coord: 125..154
score: 1.
NoneNo IPR availablePANTHERPTHR11351ACYL-COA DESATURASEcoord: 27..156
score: 3.1E-167coord: 184..337
score: 3.1E
NoneNo IPR availablePANTHERPTHR11351:SF2DELTA-9 ACYL-LIPID DESATURASE 1-RELATEDcoord: 184..337
score: 3.1E-167coord: 27..156
score: 3.1E