CSPI01G20940.1 (mRNA) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI01G20940.1
Type	mRNA
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	DNA polymerase epsilon subunit 2
Location	Chr1 : 16513589 .. 16514830 (+)
Sequence length	681

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACACATTAACTTTGAGGAAAAAGGTACAGAGGAAAGCTAAAATCAGAGGGTTGTACAGCATCAAACTGGAAGCTCTTGACGAGATTGTTTCCTTTGTCTCTCGTTCCCATGGCTTCGAAGATGAAGCTATAGAATTTGTTCTTGACAATCTCCATGAAGAATCTTGTACTGTTCCTTCTTCTTTCATACTACGATTATATCTTCTTTGCAATTCACCTCATTCCTTTTCATGGACAGTTTCTTTGTTTTGGCTATTGTGGCAGTGAAATCTCCAATATTAGACAAGGATGCGGTGCATCGAGTTATCAGCATTATGGTTGCAGCTGATAAAGTGGGAGAAGAAAGTCCCAATACCATTACCAGTACATCTGCTCTTTGCATCATCAACGCTTTTGATATCCCCAAGTTTCGTTATGATCCCATAAAAAAGATCTTTCTGTATGGTTTTCTCCCATTTATATTTATTTTTAATAGTTGTTAAACTAAGCATACCTGGTTAAGCATATACCTTGGGCCAAGAAGTCAGCAAAATCTCTCATTCCCATGTGTTGTTGAATACTTGAACTAAAAAAAAACTACTAGTTGGGTTTTTTGGGATAATTAGACGGGATAACTAATGCAGCTCATTGCAAAAAAGAGAGATATAATGGATTTAACTGAGAACTATCATCATCAACATTCCTTTTCTCTGCACTTGAAATCACATGTTCTTGAACCATGTACATATATATTTTTAGGCACACAGAAAACCTTCCAATTCATGGAGATGCTTCTGCTAAAGCTGCTTTATATAGAGATAGGTTTCTTTTGCTTTCCCAAAGGCTCTCTCGAGATCAGCATTTCTCCAAACCTGCTTTTGACATTGGCATGTCTCATTTTGGAAGTTGTGAGGTACTCCATTTCTCTTGACCTATGAATTGATCATGTTAGCCTTTACAATTGAACATGAGCATTTACACTAATTTTGTTTGTGATGTGTTTCCCAGATTTCTCCTATTCAATCTTTGGTGGGGCAAACAGGGAGAAAATGGGTCATGGGAGTGATCTCTCAAATGGAGGATGGCCACTTCTACTTGGAAGACCTTACCGCATCTGTGGAAATTAATTTATCTAGTGCTATATCCTTTACTGGCAACTTCTATGATGTGGAAAAATCTGAACTTGTTTGATATTTGAAGATGACATGCCCTGCAGTTTTCATTAATAGTTAGCACAAGATAACTACAGGTCTATTTACA

mRNA sequence

ATGGACACATTAACTTTGAGGAAAAAGGTACAGAGGAAAGCTAAAATCAGAGGGTTGTACAGCATCAAACTGGAAGCTCTTGACGAGATTGTTTCCTTTGTCTCTCGTTCCCATGGCTTCGAAGATGAAGCTATAGAATTTGTTCTTGACAATCTCCATGAAGAATCTTTGAAATCTCCAATATTAGACAAGGATGCGGTGCATCGAGTTATCAGCATTATGGTTGCAGCTGATAAAGTGGGAGAAGAAAGTCCCAATACCATTACCAGTACATCTGCTCTTTGCATCATCAACGCTTTTGATATCCCCAAGTTTCGTTATGATCCCATAAAAAAGATCTTTCTGCACACAGAAAACCTTCCAATTCATGGAGATGCTTCTGCTAAAGCTGCTTTATATAGAGATAGGTTTCTTTTGCTTTCCCAAAGGCTCTCTCGAGATCAGCATTTCTCCAAACCTGCTTTTGACATTGGCATGTCTCATTTTGGAAGTTGTGAGATTTCTCCTATTCAATCTTTGGTGGGGCAAACAGGGAGAAAATGGGTCATGGGAGTGATCTCTCAAATGGAGGATGGCCACTTCTACTTGGAAGACCTTACCGCATCTGTGGAAATTAATTTATCTAGTGCTATATCCTTTACTGGCAACTTCTATGATGTGGAAAAATCTGAACTTGTTTGA

Coding sequence (CDS)

ATGGACACATTAACTTTGAGGAAAAAGGTACAGAGGAAAGCTAAAATCAGAGGGTTGTACAGCATCAAACTGGAAGCTCTTGACGAGATTGTTTCCTTTGTCTCTCGTTCCCATGGCTTCGAAGATGAAGCTATAGAATTTGTTCTTGACAATCTCCATGAAGAATCTTTGAAATCTCCAATATTAGACAAGGATGCGGTGCATCGAGTTATCAGCATTATGGTTGCAGCTGATAAAGTGGGAGAAGAAAGTCCCAATACCATTACCAGTACATCTGCTCTTTGCATCATCAACGCTTTTGATATCCCCAAGTTTCGTTATGATCCCATAAAAAAGATCTTTCTGCACACAGAAAACCTTCCAATTCATGGAGATGCTTCTGCTAAAGCTGCTTTATATAGAGATAGGTTTCTTTTGCTTTCCCAAAGGCTCTCTCGAGATCAGCATTTCTCCAAACCTGCTTTTGACATTGGCATGTCTCATTTTGGAAGTTGTGAGATTTCTCCTATTCAATCTTTGGTGGGGCAAACAGGGAGAAAATGGGTCATGGGAGTGATCTCTCAAATGGAGGATGGCCACTTCTACTTGGAAGACCTTACCGCATCTGTGGAAATTAATTTATCTAGTGCTATATCCTTTACTGGCAACTTCTATGATGTGGAAAAATCTGAACTTGTTTGA

Homology

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BLAST of CSPI01G20940.1 vs. Swiss-Prot
Match: DPB2_ARATH (DNA polymerase epsilon subunit B OS=Arabidopsis thaliana GN=DPB2 PE=1 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 4.7e-56
Identity = 123/213 (57.75%), Postives = 158/213 (74.18%), Query Frame = 1


Query: 7   RKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFED-EAIEFVLDNLHEESLKSPILDKD 66
           RKK+Q+K K RG Y++K +ALDEI+ F  +    +D EAI+ +LDNL +E+ KS  +D +
Sbjct: 8   RKKIQKKFKNRG-YNLKFDALDEILVFADQFPDDDDGEAIDLLLDNL-QETHKSSTVDAE 67

Query: 67  AVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFL-HTENLPIHG 126
           +V  +I+ ++ A    EE     TS S+L II+AF +PKF YD +KK F  HT +LPIHG
Sbjct: 68  SVRGLINRLLGAHNAPEEPT---TSASSLAIIDAFLVPKFGYDSVKKKFNEHTSSLPIHG 127

Query: 127 DASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMG 186
           +ASAK ALYR+RF+LLSQR+SR +HFS+PAFD  MS F + EIS IQSL+ Q GRKWVMG
Sbjct: 128 EASAKTALYRERFMLLSQRVSRAEHFSRPAFDAEMSQFENNEISSIQSLISQRGRKWVMG 187

Query: 187 VISQMEDGHFYLEDLTASVEINLSSAISFTGNF 218
           VISQ+EDGHFYLEDL+ASVEI+LS A   TG F
Sbjct: 188 VISQLEDGHFYLEDLSASVEIDLSKAKITTGFF 215

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BLAST of CSPI01G20940.1 vs. Swiss-Prot
Match: DPOE2_DICDI (DNA polymerase epsilon subunit 2 OS=Dictyostelium discoideum GN=pole2 PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.5e-22
Identity = 68/207 (32.85%), Postives = 120/207 (57.97%), Query Frame = 1


Query: 7   RKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSPILDK-D 66
           +K++ +   + G +SIK +A+  I++ + ++ G++   I  ++DNL +   KS  L   +
Sbjct: 6   KKQITKSFSLTG-FSIKADAMKCIINRI-KNEGYD---ITQLIDNLLKNLDKSQYLQTIE 65

Query: 67  AVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENLP--IH 126
              ++I  + ++D + +E         AL +I+AF+ P F +D   K F+ +EN    +H
Sbjct: 66  GCFKLIDTINSSDAIEKE---------ALKVIDAFNTPLFNFDSNSKQFIKSENFDRSLH 125

Query: 127 GDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVM 186
           G +++K+ LYR R+L + QR  R+ +FS P             I+P+ SL+G TGRK V+
Sbjct: 126 GSSNSKSDLYRKRYLKVLQRTERNDYFSTPVLASDKLKNEYQTITPLSSLLGNTGRKHVL 185

Query: 187 GVISQMEDGHFYLEDLTASVEINLSSA 211
           G ISQ+E+  FY+EDL  +V+I++S A
Sbjct: 186 GTISQIEEDQFYIEDLNTNVKIDISKA 198

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BLAST of CSPI01G20940.1 vs. Swiss-Prot
Match: DPOE2_BOVIN (DNA polymerase epsilon subunit 2 OS=Bos taurus GN=POLE2 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.8e-16
Identity = 66/220 (30.00%), Postives = 117/220 (53.18%), Query Frame = 1


Query: 6   LRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFE-DEAIEFVLDNLHEESLKSPILDK 65
           LR +     K+RGL  ++ EA+  +   +   +  E ++A+E ++D + ++ L S ++++
Sbjct: 6   LRSRALSAFKLRGLM-LRGEAVKYLTEALQSINEIELEDALEKIIDAVEKQPLSSNMIER 65

Query: 66  DAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHT--ENLP- 125
             V    ++   +  V E      T      II AFDIP+F Y+  +K FL     N P 
Sbjct: 66  SVVEA--AVQECSQSVDE------TIDHIFNIIGAFDIPRFVYNSERKKFLPLLMTNHPA 125

Query: 126 --IHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGS-CEISPIQSLVGQT- 185
             + G A  KA L+R+R+ +L QR  R + F+ P         GS  ++  I++L+G T 
Sbjct: 126 PNLFGTARDKAELFRERYTILHQRTHRHELFTPPVIGSHPDESGSKFQLKTIETLLGSTS 185

Query: 186 --GRKWVMGVISQMEDGHFYLEDLTASVEINLSSAISFTG 216
             G   V+G+I+Q+++G F+LED T +++++LS A   +G
Sbjct: 186 KIGDVIVLGMITQLKEGKFFLEDPTGTIQLDLSKAQFHSG 216

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BLAST of CSPI01G20940.1 vs. Swiss-Prot
Match: DPOE2_CHICK (DNA polymerase epsilon subunit 2 OS=Gallus gallus GN=POLE2 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 5.3e-15
Identity = 63/228 (27.63%), Postives = 121/228 (53.07%), Query Frame = 1


Query: 1   MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFE-DEAIEFVLDNLHEESLKS 60
           M+   LR+++    ++RGL  ++ EA+  +   +   +  E ++ I+ ++D + ++ L +
Sbjct: 1   MEPERLRRRLGSAFRVRGLL-LRAEAVKYLTETLESLNEEEFEDVIDKIIDAVEKQPLST 60

Query: 61  PILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIF--LHT 120
            +++   V    ++   +  + E   N         II AFDIP++ Y+P +K F  L  
Sbjct: 61  NMIELSTVEA--AVQECSQALDETIKNVFN------IIGAFDIPRYIYNPDRKRFVPLSM 120

Query: 121 ENLPI---HGDASAKAALYRDRFLLLSQRLSRDQHFSKPAF----DIGMSHFGSCEISPI 180
            NLP+    G A  KA L+R+R+ +L QR  R + F+  A     D   S F   ++  +
Sbjct: 121 TNLPVPNLFGTARDKAELFRERYSILQQRTHRHELFTPAAVVIHPDDNRSKF---QLKTV 180

Query: 181 QSLVGQTGRKW---VMGVISQMEDGHFYLEDLTASVEINLSSAISFTG 216
           ++L+G T +     V+G+I+Q+++G ++LED T  V+++LS A   +G
Sbjct: 181 ETLLGNTAKVREVIVLGMITQLKEGKYFLEDPTGVVQLDLSKAQFHSG 216

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BLAST of CSPI01G20940.1 vs. Swiss-Prot
Match: DPOE2_MOUSE (DNA polymerase epsilon subunit 2 OS=Mus musculus GN=Pole2 PE=2 SV=2)

HSP 1 Score: 82.4 bits (202), Expect = 6.9e-15
Identity = 64/220 (29.09%), Postives = 110/220 (50.00%), Query Frame = 1


Query: 6   LRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFE-DEAIEFVLDNLHEESLKSPILDK 65
           LR +     K+RGL  ++ EA   +   +      E +E +  ++D + ++ L S ++++
Sbjct: 6   LRSRTVSAFKLRGLL-LRSEATKYLTEALQSVTELELEENLGKIIDAVEKQPLSSNMIER 65

Query: 66  DAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL---- 125
             V        A  +  +    TI       II AFDIP+F Y+  +K FL         
Sbjct: 66  SVVE------AAVQECSQSMDETIEHV--FNIIGAFDIPRFIYNSERKKFLPLSMTNHAA 125

Query: 126 -PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGS-CEISPIQSLVGQT- 185
             + G A  KA LY +R+ +L QR  R + F+ P     +   GS  ++  I++L+G T 
Sbjct: 126 PTLFGTARDKAELYLERYTILHQRTHRHELFTPPVIGSHLEESGSKFQLKTIETLLGSTS 185

Query: 186 --GRKWVMGVISQMEDGHFYLEDLTASVEINLSSAISFTG 216
             G   V+G+I+Q+++G F+LED T +V+++LS A   +G
Sbjct: 186 KVGDVIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSG 216

[Close]

BLAST of CSPI01G20940.1 vs. TrEMBL
Match: A0A0A0LUV9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G434690 PE=4 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 9.1e-123
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 1


Query: 1   MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP 60
           MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP
Sbjct: 1   MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP 60

Query: 61  ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL 120
           ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL
Sbjct: 61  ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL 120

Query: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK 180
           PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK 180

Query: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAISFTGNFYDVEKSELV 227
           WVMGVISQMEDGHFYLEDLTASVEINLSSAISFTGNFYDVEKSELV
Sbjct: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAISFTGNFYDVEKSELV 226

[Close]

BLAST of CSPI01G20940.1 vs. TrEMBL
Match: A5AMH8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_038180 PE=4 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 3.2e-75
Identity = 149/209 (71.29%), Postives = 178/209 (85.17%), Query Frame = 1


Query: 7   RKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSPILDKDA 66
           RKKVQRK KIRG Y++K+EALDEI+SF+S     EDEAI+ +LD L  ESLKS ILDK++
Sbjct: 6   RKKVQRKFKIRG-YTLKVEALDEILSFLSHFQDAEDEAIDLLLDELENESLKSSILDKES 65

Query: 67  VHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFL-HTENLPIHGD 126
           VHRV+S+++ A+   EE+P  I++ SAL I++AF + KF YDPIKKIF  +T  L IHGD
Sbjct: 66  VHRVVSLLLEAEAAVEETPGAISTRSALRIVDAFLMSKFCYDPIKKIFYENTGRLAIHGD 125

Query: 127 ASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMGV 186
           ASAK+ALYR+RFLLL QRLSRDQHFSKPAFD  MSHFGSCEISPIQSL+GQTG++WVMGV
Sbjct: 126 ASAKSALYRNRFLLLFQRLSRDQHFSKPAFDTEMSHFGSCEISPIQSLIGQTGKRWVMGV 185

Query: 187 ISQMEDGHFYLEDLTASVEINLSSAISFT 215
           ISQ+EDGHF+LEDLTASVEINLS+AISFT
Sbjct: 186 ISQLEDGHFFLEDLTASVEINLSNAISFT 213

[Close]

BLAST of CSPI01G20940.1 vs. TrEMBL
Match: A0A161ZS40_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_023267 PE=4 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 3.6e-71
Identity = 137/212 (64.62%), Postives = 176/212 (83.02%), Query Frame = 1


Query: 6   LRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSPILDKD 65
           +RK +Q+K K+RG Y++K+EAL+EI+ F+S+    EDEA++ +LD L  +SLKS ILDK+
Sbjct: 5   MRKLIQKKFKMRG-YTLKVEALNEILPFLSKFKDAEDEALDLLLDELQHQSLKSSILDKE 64

Query: 66  AVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFL-HTENLPIHG 125
           +V RV+S+++ A+   E++P + ++T AL II+AF +PK+RYDPIKKIFL HT  LPIHG
Sbjct: 65  SVSRVVSLLLEAEAAAEDTPASTSATGALRIIDAFIVPKYRYDPIKKIFLEHTGRLPIHG 124

Query: 126 DASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMG 185
           DASAKA LYRDRFLLL QRLSRD HFS+PAFD  +S FG+C+ISPIQSLVG+TG+ WVMG
Sbjct: 125 DASAKATLYRDRFLLLFQRLSRDPHFSRPAFDTDLSQFGNCQISPIQSLVGRTGKCWVMG 184

Query: 186 VISQMEDGHFYLEDLTASVEINLSSAISFTGN 217
           VISQ+EDGHFYLEDLTA+VEINLS+AIS   N
Sbjct: 185 VISQLEDGHFYLEDLTAAVEINLSNAISLPAN 215

[Close]

BLAST of CSPI01G20940.1 vs. TrEMBL
Match: A0A059AW13_EUCGR (DNA polymerase epsilon subunit 2 OS=Eucalyptus grandis GN=EUGRSUZ_H00594 PE=3 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 8.1e-71
Identity = 141/215 (65.58%), Postives = 175/215 (81.40%), Query Frame = 1


Query: 6   LRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSPILDKD 65
           ++KKVQRK K+RG Y+++++ALD ++SF SR  G ED+A++ +LD L  ESLKS I+DKD
Sbjct: 5   MKKKVQRKFKMRG-YTLRVDALDAVLSFASRFAGAEDDALDLLLDQLDHESLKSSIIDKD 64

Query: 66  AVHRVISIMVAADKVGEESPNTITST--SALCIINAFDIPKFRYDPIKKIFL-HTENLPI 125
            V+RV+S+++ A+   EESP+       SAL +I+AF +PKFRYDP+KKIF  HT +LPI
Sbjct: 65  PVNRVVSLLLEAEAAVEESPDAACEGRGSALRVIDAFLVPKFRYDPVKKIFYEHTGSLPI 124

Query: 126 HGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWV 185
           HG+ASAKA LY+DRFLLL QRLSRDQHF+KPAF+   + FGSCEISPIQSLVGQTGR+WV
Sbjct: 125 HGEASAKAKLYKDRFLLLYQRLSRDQHFAKPAFETETTEFGSCEISPIQSLVGQTGRRWV 184

Query: 186 MGVISQMEDGHFYLEDLTASVEINLSSAISFTGNF 218
           MGVISQ+EDGHFYLEDLTASVEINLS A   TG F
Sbjct: 185 MGVISQLEDGHFYLEDLTASVEINLSDAKVTTGFF 218

[Close]

BLAST of CSPI01G20940.1 vs. TrEMBL
Match: D7SIP4_VITVI (DNA polymerase epsilon subunit 2 OS=Vitis vinifera GN=VIT_17s0000g04830 PE=3 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 9.0e-70
Identity = 141/213 (66.20%), Postives = 172/213 (80.75%), Query Frame = 1


Query: 7   RKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSPILDKDA 66
           RKKVQRK KIRG Y++K+EALDEI+SF+S     EDEAI+ +LD L  ESLKS ILDK++
Sbjct: 6   RKKVQRKFKIRG-YTLKVEALDEILSFLSHFQDAEDEAIDLLLDELENESLKSSILDKES 65

Query: 67  VHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFL-HTENLPIHGD 126
           VHRV+S+++ A+   EE+P  I++ SAL I++AF + KF YDPIKKIF  +T  L IHGD
Sbjct: 66  VHRVVSLLLEAEAAVEETPGAISTRSALRIVDAFLMSKFCYDPIKKIFYENTGRLAIHGD 125

Query: 127 ASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMGV 186
           ASAK+ALYR+RFLLL QRLSRDQHFSKPAFD  MSHFGSCEISPIQSL+GQTG++WVMGV
Sbjct: 126 ASAKSALYRNRFLLLFQRLSRDQHFSKPAFDTEMSHFGSCEISPIQSLIGQTGKRWVMGV 185

Query: 187 ISQMEDGHFYLEDLTASVEINLSSAISFTGNFY 219
           ISQ+EDGHF+LEDLTAS+ + L      T  F+
Sbjct: 186 ISQLEDGHFFLEDLTASLAL-LHQQYKITTGFF 216

[Close]

BLAST of CSPI01G20940.1 vs. TAIR10
Match: AT5G22110.1 (AT5G22110.1 DNA polymerase epsilon subunit B2)

HSP 1 Score: 219.2 bits (557), Expect = 2.7e-57
Identity = 123/213 (57.75%), Postives = 158/213 (74.18%), Query Frame = 1


Query: 7   RKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFED-EAIEFVLDNLHEESLKSPILDKD 66
           RKK+Q+K K RG Y++K +ALDEI+ F  +    +D EAI+ +LDNL +E+ KS  +D +
Sbjct: 8   RKKIQKKFKNRG-YNLKFDALDEILVFADQFPDDDDGEAIDLLLDNL-QETHKSSTVDAE 67

Query: 67  AVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFL-HTENLPIHG 126
           +V  +I+ ++ A    EE     TS S+L II+AF +PKF YD +KK F  HT +LPIHG
Sbjct: 68  SVRGLINRLLGAHNAPEEPT---TSASSLAIIDAFLVPKFGYDSVKKKFNEHTSSLPIHG 127

Query: 127 DASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMG 186
           +ASAK ALYR+RF+LLSQR+SR +HFS+PAFD  MS F + EIS IQSL+ Q GRKWVMG
Sbjct: 128 EASAKTALYRERFMLLSQRVSRAEHFSRPAFDAEMSQFENNEISSIQSLISQRGRKWVMG 187

Query: 187 VISQMEDGHFYLEDLTASVEINLSSAISFTGNF 218
           VISQ+EDGHFYLEDL+ASVEI+LS A   TG F
Sbjct: 188 VISQLEDGHFYLEDLSASVEIDLSKAKITTGFF 215

[Close]

BLAST of CSPI01G20940.1 vs. NCBI nr
Match: gi|700210427|gb|KGN65523.1| (hypothetical protein Csa_1G434690 [Cucumis sativus])

HSP 1 Score: 447.6 bits (1150), Expect = 1.3e-122
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 1


Query: 1   MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP 60
           MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP
Sbjct: 1   MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP 60

Query: 61  ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL 120
           ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL
Sbjct: 61  ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL 120

Query: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK 180
           PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK 180

Query: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAISFTGNFYDVEKSELV 227
           WVMGVISQMEDGHFYLEDLTASVEINLSSAISFTGNFYDVEKSELV
Sbjct: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAISFTGNFYDVEKSELV 226

[Close]

BLAST of CSPI01G20940.1 vs. NCBI nr
Match: gi|778660923|ref|XP_011657177.1| (PREDICTED: DNA polymerase epsilon subunit 2 [Cucumis sativus])

HSP 1 Score: 418.3 bits (1074), Expect = 8.5e-114
Identity = 213/217 (98.16%), Postives = 213/217 (98.16%), Query Frame = 1


Query: 1   MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP 60
           MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP
Sbjct: 1   MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP 60

Query: 61  ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL 120
           ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL
Sbjct: 61  ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL 120

Query: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK 180
           PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK 180

Query: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAISFTGNF 218
           WVMGVISQMEDGHFYLEDLTASVEINLSSA   TG F
Sbjct: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAKITTGLF 217

[Close]

BLAST of CSPI01G20940.1 vs. NCBI nr
Match: gi|659066275|ref|XP_008463560.1| (PREDICTED: LOW QUALITY PROTEIN: DNA polymerase epsilon subunit 2 [Cucumis melo])

HSP 1 Score: 369.8 bits (948), Expect = 3.5e-99
Identity = 190/218 (87.16%), Postives = 201/218 (92.20%), Query Frame = 1


Query: 1   MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP 60
           MD LTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSR HGFED+AIE VLD+LHEESLKSP
Sbjct: 1   MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 60

Query: 61  ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIF-LHTEN 120
           IL+KD VHRVIS ++AA++  E SPNTITST+ALCII+AFDI KFRYDPIKKIF  HT +
Sbjct: 61  ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDAFDISKFRYDPIKKIFHRHTGD 120

Query: 121 LPIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 180
           LPIHGDA AKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR
Sbjct: 121 LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 180

Query: 181 KWVMGVISQMEDGHFYLEDLTASVEINLSSAISFTGNF 218
           KWVMGVISQMEDGHFYLEDLTASVEINLS+A   TG F
Sbjct: 181 KWVMGVISQMEDGHFYLEDLTASVEINLSNAKITTGLF 218

[Close]

BLAST of CSPI01G20940.1 vs. NCBI nr
Match: gi|147818232|emb|CAN73546.1| (hypothetical protein VITISV_038180 [Vitis vinifera])

HSP 1 Score: 289.7 bits (740), Expect = 4.6e-75
Identity = 149/209 (71.29%), Postives = 178/209 (85.17%), Query Frame = 1


Query: 7   RKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSPILDKDA 66
           RKKVQRK KIRG Y++K+EALDEI+SF+S     EDEAI+ +LD L  ESLKS ILDK++
Sbjct: 6   RKKVQRKFKIRG-YTLKVEALDEILSFLSHFQDAEDEAIDLLLDELENESLKSSILDKES 65

Query: 67  VHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFL-HTENLPIHGD 126
           VHRV+S+++ A+   EE+P  I++ SAL I++AF + KF YDPIKKIF  +T  L IHGD
Sbjct: 66  VHRVVSLLLEAEAAVEETPGAISTRSALRIVDAFLMSKFCYDPIKKIFYENTGRLAIHGD 125

Query: 127 ASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMGV 186
           ASAK+ALYR+RFLLL QRLSRDQHFSKPAFD  MSHFGSCEISPIQSL+GQTG++WVMGV
Sbjct: 126 ASAKSALYRNRFLLLFQRLSRDQHFSKPAFDTEMSHFGSCEISPIQSLIGQTGKRWVMGV 185

Query: 187 ISQMEDGHFYLEDLTASVEINLSSAISFT 215
           ISQ+EDGHF+LEDLTASVEINLS+AISFT
Sbjct: 186 ISQLEDGHFFLEDLTASVEINLSNAISFT 213

[Close]

BLAST of CSPI01G20940.1 vs. NCBI nr
Match: gi|225455720|ref|XP_002267181.1| (PREDICTED: DNA polymerase epsilon subunit 2 [Vitis vinifera])

HSP 1 Score: 285.4 bits (729), Expect = 8.6e-74
Identity = 148/212 (69.81%), Postives = 177/212 (83.49%), Query Frame = 1


Query: 7   RKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSPILDKDA 66
           RKKVQRK KIRG Y++K+EALDEI+SF+S     EDEAI+ +LD L  ESLKS ILDK++
Sbjct: 6   RKKVQRKFKIRG-YTLKVEALDEILSFLSHFQDAEDEAIDLLLDELENESLKSSILDKES 65

Query: 67  VHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFL-HTENLPIHGD 126
           VHRV+S+++ A+   EE+P  I++ SAL I++AF + KF YDPIKKIF  +T  L IHGD
Sbjct: 66  VHRVVSLLLEAEAAVEETPGAISTRSALRIVDAFLMSKFCYDPIKKIFYENTGRLAIHGD 125

Query: 127 ASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMGV 186
           ASAK+ALYR+RFLLL QRLSRDQHFSKPAFD  MSHFGSCEISPIQSL+GQTG++WVMGV
Sbjct: 126 ASAKSALYRNRFLLLFQRLSRDQHFSKPAFDTEMSHFGSCEISPIQSLIGQTGKRWVMGV 185

Query: 187 ISQMEDGHFYLEDLTASVEINLSSAISFTGNF 218
           ISQ+EDGHF+LEDLTASVEINLS+A   TG F
Sbjct: 186 ISQLEDGHFFLEDLTASVEINLSNAKITTGFF 216

The following BLAST results are available for this feature:

BLAST of CSPI01G20940.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
DPB2_ARATH	4.7e-56	57.75	DNA polymerase epsilon subunit B OS=Arabidopsis thaliana GN=DPB2 PE=1 SV=1	[more]
DPOE2_DICDI	1.5e-22	32.85	DNA polymerase epsilon subunit 2 OS=Dictyostelium discoideum GN=pole2 PE=3 SV=1	[more]
DPOE2_BOVIN	2.8e-16	30.00	DNA polymerase epsilon subunit 2 OS=Bos taurus GN=POLE2 PE=2 SV=1	[more]
DPOE2_CHICK	5.3e-15	27.63	DNA polymerase epsilon subunit 2 OS=Gallus gallus GN=POLE2 PE=2 SV=1	[more]
DPOE2_MOUSE	6.9e-15	29.09	DNA polymerase epsilon subunit 2 OS=Mus musculus GN=Pole2 PE=2 SV=2	[more]

BLAST of CSPI01G20940.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LUV9_CUCSA	9.1e-123	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G434690 PE=4 SV=1	[more]
A5AMH8_VITVI	3.2e-75	71.29	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_038180 PE=4 SV=1	[more]
A0A161ZS40_DAUCA	3.6e-71	64.62	Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_023267 PE=4 SV=1	[more]
A0A059AW13_EUCGR	8.1e-71	65.58	DNA polymerase epsilon subunit 2 OS=Eucalyptus grandis GN=EUGRSUZ_H00594 PE=3 SV...	[more]
D7SIP4_VITVI	9.0e-70	66.20	DNA polymerase epsilon subunit 2 OS=Vitis vinifera GN=VIT_17s0000g04830 PE=3 SV=...	[more]

BLAST of CSPI01G20940.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G22110.1	2.7e-57	57.75	DNA polymerase epsilon subunit B2	[more]

BLAST of CSPI01G20940.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700210427\|gb\|KGN65523.1\|	1.3e-122	100.00	hypothetical protein Csa_1G434690 [Cucumis sativus]	[more]
gi\|778660923\|ref\|XP_011657177.1\|	8.5e-114	98.16	PREDICTED: DNA polymerase epsilon subunit 2 [Cucumis sativus]	[more]
gi\|659066275\|ref\|XP_008463560.1\|	3.5e-99	87.16	PREDICTED: LOW QUALITY PROTEIN: DNA polymerase epsilon subunit 2 [Cucumis melo]	[more]
gi\|147818232\|emb\|CAN73546.1\|	4.6e-75	71.29	hypothetical protein VITISV_038180 [Vitis vinifera]	[more]
gi\|225455720\|ref\|XP_002267181.1\|	8.6e-74	69.81	PREDICTED: DNA polymerase epsilon subunit 2 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071897	DNA biosynthetic process
biological_process	GO:0006261	DNA-dependent DNA replication
cellular_component	GO:0008622	epsilon DNA polymerase complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003887	DNA-directed DNA polymerase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI01G20940	CSPI01G20940	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI01G20940.1	CSPI01G20940.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI01G20940.1.cds1	CSPI01G20940.1.cds1	CDS
CSPI01G20940.1.cds2	CSPI01G20940.1.cds2	CDS
CSPI01G20940.1.cds3	CSPI01G20940.1.cds3	CDS
CSPI01G20940.1.cds4	CSPI01G20940.1.cds4	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI01G20940.1.utr3p1	CSPI01G20940.1.utr3p1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR12708	DNA POLYMERASE EPSILON SUBUNIT B	coord: 1..218 score: 2.9
None	No IPR available	PANTHER	PTHR12708:SF0	DNA POLYMERASE EPSILON SUBUNIT 2	coord: 1..218 score: 2.9