CSPI01G16570.1 (mRNA) Wild cucumber (PI 183967)

NameCSPI01G16570.1
TypemRNA
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionTIR-NBS-LRR disease resistance protein
LocationChr1 : 12123548 .. 12124652 (+)
Sequence length1005
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATGGCATTGGAGGCACTGGTAAGACCACTTTGGCTAAGGATTTGTACAACAAAATTGCTACCCAATTTGAAAGATGCTACTTTCTACGAGATGTTAGACGAGAAGCTTCAAAGCAATATGGGCTCGTTCAACTACAGGAAACCTTACTCTGTGAGATTTTAAAGGAGGATTTGAAGGTTGTCAATTGTGATAAAGGAATTAACATCATAAGGAGTAGACTCTGTTTAAAGAAAGTTCTTATAGTTCTTGATGATGTGGATCATCACGAGCAATTAGAAGCACCAGTTGGTGAGCACGATTGGTTTGGTCGAGGTAGTAAAATCATTATGTTAACAAGGAATGGACATTTACTTTCAAGCCATGGATTCGATGAAAAGCATAAAATTCATGAATTGGATCAAGACCATGCTCTTGTTCTTTTTAGTTGGCATGCTTTCAAAAAAAGTCATCCATCAAGTAGTTATATAGGCCTTTCAGAAAGTGCTACAAATTATTGTAAAGGTCTCTCTGTGGCACTCGTTGTTTTGGGTTCTTTCCTTCGTGGTAGAGATCAAACAAGATGGAGTTGTATATTAGATGAATTTGAAAACTCTCTGCCAAAAGATATTAAAGATGTTCTTCAATTAAGTTTTGATGGACTAGAAGACAAAGCAAACGATATCTTTCTTGATATTTCTTGTTTATTTGTGGGAGAAGATTACAATTGTGCTTTAAAAAAAATGTTGAGTGCATGTCATTCGAACGTAGATTTTGGAATTATGATTCTCATGGATCTTTCACTTATTATGATTGAAAGTGATAGAGTGCAAATGCATGGATTAATACAACAAATGGGTCGTAGCTTAGTTCGTAATGAATCATCTCAACCTGGAAAGAGGAGTAGGTTGTGGTTGGTTGAAGACATTGGGGAGGTGTTTGTTAATAAGTCTGTGAGAAACTCTTTACCTAAAGTATTGATAGTTTACTTATTTTTAGGACTTCACGTTATAAGAAATATTTGTTACAGTAAGTTGTCAGTAATGTTAAATTATTAAATCTATGATTTTGTAGGGAACAGATGCAATTAAAGCTATAAAGTCGGACTTGCCTAATCCCACATGA

mRNA sequence

ATGTATGGCATTGGAGGCACTGGTAAGACCACTTTGGCTAAGGATTTGTACAACAAAATTGCTACCCAATTTGAAAGATGCTACTTTCTACGAGATGTTAGACGAGAAGCTTCAAAGCAATATGGGCTCGTTCAACTACAGGAAACCTTACTCTGTGAGATTTTAAAGGAGGATTTGAAGGTTGTCAATTGTGATAAAGGAATTAACATCATAAGGAGTAGACTCTGTTTAAAGAAAGTTCTTATAGTTCTTGATGATGTGGATCATCACGAGCAATTAGAAGCACCAGTTGGTGAGCACGATTGGTTTGGTCGAGGTAGTAAAATCATTATGTTAACAAGGAATGGACATTTACTTTCAAGCCATGGATTCGATGAAAAGCATAAAATTCATGAATTGGATCAAGACCATGCTCTTGTTCTTTTTAGTTGGCATGCTTTCAAAAAAAGTCATCCATCAAGTAGTTATATAGGCCTTTCAGAAAGTGCTACAAATTATTGTAAAGGTCTCTCTGTGGCACTCGTTGTTTTGGGTTCTTTCCTTCGTGGTAGAGATCAAACAAGATGGAGTTGTATATTAGATGAATTTGAAAACTCTCTGCCAAAAGATATTAAAGATGTTCTTCAATTAAGTTTTGATGGACTAGAAGACAAAGCAAACGATATCTTTCTTGATATTTCTTGTTTATTTGTGGGAGAAGATTACAATTGTGCTTTAAAAAAAATGTTGAGTGCATGTCATTCGAACGTAGATTTTGGAATTATGATTCTCATGGATCTTTCACTTATTATGATTGAAAGTGATAGAGTGCAAATGCATGGATTAATACAACAAATGGGTCGTAGCTTAGTTCGTAATGAATCATCTCAACCTGGAAAGAGGAGTAGGTTGTGGTTGGTTGAAGACATTGGGGAGGTGTTTGTTAATAAGTCTGTGAGAAACTCTTTACCTAAAGGAACAGATGCAATTAAAGCTATAAAGTCGGACTTGCCTAATCCCACATGA

Coding sequence (CDS)

ATGTATGGCATTGGAGGCACTGGTAAGACCACTTTGGCTAAGGATTTGTACAACAAAATTGCTACCCAATTTGAAAGATGCTACTTTCTACGAGATGTTAGACGAGAAGCTTCAAAGCAATATGGGCTCGTTCAACTACAGGAAACCTTACTCTGTGAGATTTTAAAGGAGGATTTGAAGGTTGTCAATTGTGATAAAGGAATTAACATCATAAGGAGTAGACTCTGTTTAAAGAAAGTTCTTATAGTTCTTGATGATGTGGATCATCACGAGCAATTAGAAGCACCAGTTGGTGAGCACGATTGGTTTGGTCGAGGTAGTAAAATCATTATGTTAACAAGGAATGGACATTTACTTTCAAGCCATGGATTCGATGAAAAGCATAAAATTCATGAATTGGATCAAGACCATGCTCTTGTTCTTTTTAGTTGGCATGCTTTCAAAAAAAGTCATCCATCAAGTAGTTATATAGGCCTTTCAGAAAGTGCTACAAATTATTGTAAAGGTCTCTCTGTGGCACTCGTTGTTTTGGGTTCTTTCCTTCGTGGTAGAGATCAAACAAGATGGAGTTGTATATTAGATGAATTTGAAAACTCTCTGCCAAAAGATATTAAAGATGTTCTTCAATTAAGTTTTGATGGACTAGAAGACAAAGCAAACGATATCTTTCTTGATATTTCTTGTTTATTTGTGGGAGAAGATTACAATTGTGCTTTAAAAAAAATGTTGAGTGCATGTCATTCGAACGTAGATTTTGGAATTATGATTCTCATGGATCTTTCACTTATTATGATTGAAAGTGATAGAGTGCAAATGCATGGATTAATACAACAAATGGGTCGTAGCTTAGTTCGTAATGAATCATCTCAACCTGGAAAGAGGAGTAGGTTGTGGTTGGTTGAAGACATTGGGGAGGTGTTTGTTAATAAGTCTGTGAGAAACTCTTTACCTAAAGGAACAGATGCAATTAAAGCTATAAAGTCGGACTTGCCTAATCCCACATGA
BLAST of CSPI01G16570.1 vs. Swiss-Prot
Match: TMVRN_NICGU (TMV resistance protein N OS=Nicotiana glutinosa GN=N PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 4.1e-56
Identity = 131/334 (39.22%), Postives = 201/334 (60.18%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKI------ATQFERCYFLRDVRREASKQYGLVQLQETLLCEI 60
           ++G+GG GKTT+A+ +++ +      + QF+   FL+D++     + G+  LQ  LL E+
Sbjct: 214 IWGMGGVGKTTIARAIFDTLLGRMDSSYQFDGACFLKDIKEN---KRGMHSLQNALLSEL 273

Query: 61  LKEDLKVVNCDKGINIIRSRLCLKKVLIVLDDVDHHEQ-LEAPVGEHDWFGRGSKIIMLT 120
           L+E     N + G + + SRL  KKVLIVLDD+D+ +  LE   G+ DWFG GS+II+ T
Sbjct: 274 LREKANYNNEEDGKHQMASRLRSKKVLIVLDDIDNKDHYLEYLAGDLDWFGNGSRIIITT 333

Query: 121 RNGHLLSSHGFDEKHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKGLSVA 180
           R+ HL+  +  D  +++  L    ++ LF  HAF K  P+ ++  LS    NY KGL +A
Sbjct: 334 RDKHLIEKN--DIIYEVTALPDHESIQLFKQHAFGKEVPNENFEKLSLEVVNYAKGLPLA 393

Query: 181 LVVLGSFLRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGE 240
           L V GS L     T W   ++  +N+    I D L++S+DGLE K  ++FLDI+C   GE
Sbjct: 394 LKVWGSLLHNLRLTEWKSAIEHMKNNSYSGIIDKLKISYDGLEPKQQEMFLDIACFLRGE 453

Query: 241 DYNCALKKMLSACHSNVDFGIMILMDLSLIMI-ESDRVQMHGLIQQMGRSLVRNESSQPG 300
           + +  L ++L +CH   ++G+ IL+D SL+ I E ++VQMH LIQ MG+ +V N    PG
Sbjct: 454 EKDYIL-QILESCHIGAEYGLRILIDKSLVFISEYNQVQMHDLIQDMGKYIV-NFQKDPG 513

Query: 301 KRSRLWLVEDIGEVFVNKSVRNSLPKGTDAIKAI 327
           +RSRLWL +++ EV  N +       GT A++AI
Sbjct: 514 ERSRLWLAKEVEEVMSNNT-------GTMAMEAI 533

BLAST of CSPI01G16570.1 vs. Swiss-Prot
Match: Y4117_ARATH (Putative disease resistance protein At4g11170 OS=Arabidopsis thaliana GN=At4g11170 PE=2 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.3e-49
Identity = 120/338 (35.50%), Postives = 192/338 (56.80%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERCYFLRDVRREASK----QYGL-VQLQETLLCEIL 60
           ++G  G GKTT+A+ LYN+    F    F+ +VR    +     YGL + LQ+  L ++L
Sbjct: 210 IWGPAGVGKTTIARALYNQYHENFNLSIFMENVRESYGEAGLDDYGLKLHLQQRFLSKLL 269

Query: 61  -KEDLKVVNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTR 120
            ++DL+V    + +  I  RL  +KVLI+LDDVD+ EQL+A   E+ WFG  S+I++ T+
Sbjct: 270 DQKDLRV----RHLGAIEERLKSQKVLIILDDVDNIEQLKALAKENQWFGNKSRIVVTTQ 329

Query: 121 NGHLLSSHGFDEKHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKGLSVAL 180
           N  LL SH  +  +++    +  AL +F  HAFK+S PS     L+   T     L +AL
Sbjct: 330 NKQLLVSHDINHMYQVAYPSKQEALTIFCQHAFKQSSPSDDLKHLAIEFTTLAGHLPLAL 389

Query: 181 VVLGSFLRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGED 240
            VLGSF+RG+ +  W   L   ++ L  +++ VL++ +DGL D   D+FL I+C+F G+ 
Sbjct: 390 RVLGSFMRGKGKEEWEFSLPTLKSRLDGEVEKVLKVGYDGLHDHEKDLFLHIACIFSGQH 449

Query: 241 YNCALKKMLSACHSNVDFGIMILMDLSLIM-IESDRVQMHGLIQQMGRSLVRNES-SQPG 300
            N   + +++   + V FG+ +L D SLI   E+ R++MH L++Q+G+ +VR +S  +PG
Sbjct: 450 ENYLKQMIIANNDTYVSFGLQVLADKSLIQKFENGRIEMHSLLRQLGKEVVRKQSIYEPG 509

Query: 301 KRSRLWLVEDIGEVFVNKSVRNSLPKGTDAIKAIKSDL 331
           KR  L   ++   V  N +       GT  +  I  D+
Sbjct: 510 KRQFLMNAKETCGVLSNNT-------GTGTVLGISLDM 536

BLAST of CSPI01G16570.1 vs. Swiss-Prot
Match: TAO1_ARATH (Disease resistance protein TAO1 OS=Arabidopsis thaliana GN=TAO1 PE=3 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.4e-40
Identity = 107/322 (33.23%), Postives = 177/322 (54.97%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERCYFLRDVR----REASKQYGL-VQLQETLLCEIL 60
           ++G  G GKTT+++ LYNK+  QF+    + +++    R    +Y   +QLQ+ LL +++
Sbjct: 239 IWGPAGIGKTTISRVLYNKLFHQFQLGAIIDNIKVRYPRPCHDEYSAKLQLQKELLSQMI 298

Query: 61  KEDLKVVNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTRN 120
            +   VV     + + + RL  KKVL+VLDDVD   QL+A   +  WFG GS+II++T++
Sbjct: 299 NQKDMVV---PHLGVAQERLKDKKVLLVLDDVDGLVQLDAMAKDVQWFGLGSRIIVVTQD 358

Query: 121 GHLLSSHGFDEKHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKGLSVALV 180
             LL +HG    +K+     D AL +F  +AF +  P   +  ++ + T     L + L 
Sbjct: 359 LKLLKAHGIKYIYKVDFPTSDEALEIFCMYAFGEKSPKVGFEQIARTVTTLAGKLPLGLR 418

Query: 181 VLGSFLRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGEDY 240
           V+GS+LR   +  W+  +     SL  DI+ VL+ S++ L ++  D+FL I+C F  E  
Sbjct: 419 VMGSYLRRMSKQEWAKSIPRLRTSLDDDIESVLKFSYNSLAEQEKDLFLHITCFFRRERI 478

Query: 241 NCALKKMLSACHSNVDFGIMILMDLSLIMIESDRVQMHGLIQQMGRSLVRNES-SQPGKR 300
              L+  L+    ++  G+ IL D SL+ +    ++MH L+ Q+G  +VR +S  +PGKR
Sbjct: 479 E-TLEVFLAKKSVDMRQGLQILADKSLLSLNLGNIEMHNLLVQLGLDIVRKQSIHKPGKR 538

Query: 301 SRLWLVEDIGEVFVNKSVRNSL 317
             L   EDI EV  + +   +L
Sbjct: 539 QFLVDTEDICEVLTDDTGTRTL 556

BLAST of CSPI01G16570.1 vs. Swiss-Prot
Match: RLM1A_ARATH (Disease resistance protein RML1A OS=Arabidopsis thaliana GN=RLM1A PE=3 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.6e-39
Identity = 109/332 (32.83%), Postives = 180/332 (54.22%), Query Frame = 1

Query: 3   GIGGTGKTTLAKDLYNKIATQFERCYFLRDVRRE---ASKQYGL-VQLQETLLCEILKED 62
           G  G GK+T+ + L++ ++ +F    F+ ++R        +YGL ++LQE LL +IL +D
Sbjct: 215 GPAGIGKSTIGRALHSLLSNRFHHTCFVDNLRGSHPIGLDEYGLKLRLQEQLLSKILNQD 274

Query: 63  LKVVNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTRNGHL 122
              + C  G   I+ RLC  KV I+LDDV+  +QLEA   E +WFG GS+II+ T N  L
Sbjct: 275 GSRI-CHLGA--IKERLCDMKVFIILDDVNDVKQLEALANESNWFGPGSRIIVTTENKEL 334

Query: 123 LSSHGFDEKHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKGLSVALVVLG 182
           L  HG +  + +     + A+ +   +AF++S     +  L+ S T  C  L + L V+G
Sbjct: 335 LKQHGINNTYYVGFPSDEEAIKILCRYAFRQSSSRHGFKKLTRSVTELCGKLPLGLRVVG 394

Query: 183 SFLRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGEDYNCA 242
           S L G+++  W  ++   E  + +DI+ VL++ ++ L +    +FL I+  F  ED +  
Sbjct: 395 SSLHGKNEEEWEYVIRRLETIIDRDIEQVLRVGYESLHENEQSLFLHIAIFFNYEDGD-L 454

Query: 243 LKKMLSACHSNVDFGIMILMDLSLIMIESD-RVQMHGLIQQMGRSLVRNESSQPGKRSRL 302
           +K ML+    +++  + IL++ SLI I +D R++MH L+Q +GR    N+  +P KR  L
Sbjct: 455 VKAMLAENDLDIEHELNILVNKSLIYISTDGRIRMHKLLQLVGRQ--ANQREEPWKRRIL 514

Query: 303 WLVEDIGEVFVNKSVRNSLPKGTDAIKAIKSD 330
              ++I  V  N         GT A+  I  D
Sbjct: 515 IDAQEICHVLENDI-------GTGAVSGILFD 533

BLAST of CSPI01G16570.1 vs. Swiss-Prot
Match: RPS6R_ARATH (Disease resistance protein RPS6 OS=Arabidopsis thaliana GN=RPS6 PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 4.6e-39
Identity = 109/342 (31.87%), Postives = 187/342 (54.68%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERCYFLRDVRREASKQ---------YGL-VQLQETL 60
           ++G  G GKTT+++ L+++++ QF+   F+  V    S +         Y + + LQ   
Sbjct: 213 IWGPSGIGKTTISRALFSRLSCQFQSSVFIDKVFISKSMEVYSGANLVDYNMKLHLQRAF 272

Query: 61  LCEIL-KEDLKVVNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKI 120
           L EI  K+D+K+      +  +   +  +K LIV+DD+D  + L+A  G+  WFG GS+I
Sbjct: 273 LAEIFDKKDIKI-----HVGAMEKMVKHRKALIVIDDLDDQDVLDALAGQTQWFGSGSRI 332

Query: 121 IMLTRNGHLLSSHGFDEKHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKG 180
           I++T N H L ++  D  +K+       AL +F   AFKK+ P   ++ LS         
Sbjct: 333 IVVTENKHFLRANRIDHIYKVCLPSNALALEMFCRSAFKKNSPPDDFLELSSEVALRAGN 392

Query: 181 LSVALVVLGSFLRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKAND-IFLDISC 240
           L + L VLGS LRG ++  W  +L   +  L   I   L++S+DGL ++ ++ IF  I+C
Sbjct: 393 LPLGLNVLGSNLRGINKGYWIDMLPRLQ-GLDGKIGKTLRVSYDGLNNRKDEAIFRHIAC 452

Query: 241 LFVGEDYNCALKKMLSACHSNVDFGIMILMDLSLIMIESDRVQMHGLIQQMGRSLVRNES 300
           +F GE  +  +K +L+  + +V+ G+  L+D SLI    + ++MH L+Q++G+ +VR ES
Sbjct: 453 IFNGEKVS-DIKLLLANSNLDVNIGLKNLVDRSLICERFNTLEMHSLLQELGKEIVRTES 512

Query: 301 SQPGKRSRLWLVEDIGEVFVNKSVRNSLPKGTDAIKAIKSDL 331
           +QPG+R  L  ++DI +V  + +       GT  +  I  D+
Sbjct: 513 NQPGEREFLVDLKDICDVLEHNT-------GTKKVLGITLDI 540

BLAST of CSPI01G16570.1 vs. TrEMBL
Match: A0A0A0LFS4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G022790 PE=4 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.1e-137
Identity = 262/335 (78.21%), Postives = 285/335 (85.07%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERCYFLRDVRREASKQYGLVQLQETLLCEILKEDLK 60
           +YGIGG GKTTLAK LYNKIATQFE C FL+DVRREASK +GLVQLQETLL EILKEDLK
Sbjct: 223 LYGIGGIGKTTLAKALYNKIATQFEGCCFLQDVRREASK-HGLVQLQETLLNEILKEDLK 282

Query: 61  V-VNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTRNGHLL 120
           V V+ D+GINIIRSRLC KKVLIVLDDV+  EQLEA VG  DWFG+GSKII+ TRN HLL
Sbjct: 283 VIVSRDRGINIIRSRLCSKKVLIVLDDVNDLEQLEALVGGRDWFGQGSKIIVTTRNEHLL 342

Query: 121 SSHGFDEKHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKGLSVALVVLGS 180
           SSHGFDEKHKI EL+QDHAL LFSWHAFKKSHPSS+Y+  S+ AT+YCKGLS+ALVVLGS
Sbjct: 343 SSHGFDEKHKIQELNQDHALELFSWHAFKKSHPSSNYLDFSKRATSYCKGLSLALVVLGS 402

Query: 181 FLRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGEDYNCAL 240
           FL GR +  W+ +LDEFENSL KDIKDVLQLSFDGLEDK  DIFLDISCLFVGE+Y CA 
Sbjct: 403 FLCGRAKEEWNGMLDEFENSLRKDIKDVLQLSFDGLEDKIKDIFLDISCLFVGEEYKCA- 462

Query: 241 KKMLSACHSNVDFGIMILMDLSLIMIESDRVQMHGLIQQMGRSLVRNESSQPGKRSRLWL 300
           KKMLSACH N+DFGIMILMDLSLI +E DRVQMH LIQQMGRS+V NESS+PGKRSRLWL
Sbjct: 463 KKMLSACHLNIDFGIMILMDLSLITVEMDRVQMHELIQQMGRSIVHNESSEPGKRSRLWL 522

Query: 301 VEDIGEVFVNKSVRNSLPKGTDAIKAIKSDLPNPT 335
             DI EVFVN S       GTDA+KAIK DLP  T
Sbjct: 523 EHDIWEVFVNNS-------GTDAVKAIKLDLPKST 548

BLAST of CSPI01G16570.1 vs. TrEMBL
Match: A0A0A0LLL0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G020940 PE=4 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 1.7e-125
Identity = 238/333 (71.47%), Postives = 264/333 (79.28%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERCYFLRDVRREASKQYGLVQLQETLLCEILKEDLK 60
           +YGIGG GKTTLAK LYNKIA+QFE C FL +VR+ +++  GLVQLQ+ LL EIL++DLK
Sbjct: 233 IYGIGGLGKTTLAKALYNKIASQFEGCCFLSNVRQASNQFNGLVQLQQNLLYEILEDDLK 292

Query: 61  VVNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTRNGHLLS 120
            VN DKGI IIR+RL  KKVLIVLDDVD  EQLEA VG  DWFG+GSKII+ TRN HLLS
Sbjct: 293 FVNLDKGITIIRNRLRSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNSHLLS 352

Query: 121 SHGFDEKHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKGLSVALVVLGSF 180
           SHGFDE H I  L+QD A+ LFSWHAFK+SHPSS+Y+ L+E AT+YCKG  +ALVVLGSF
Sbjct: 353 SHGFDEMHNIQGLNQDRAIELFSWHAFKESHPSSNYLDLAERATSYCKGHPLALVVLGSF 412

Query: 181 LRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGEDYNCALK 240
           L  R QT W  ILD+FENSL  DIKD+LQLSFDGLE    DIFLDISCLFVGE YN   K
Sbjct: 413 LCNRGQTEWRSILDKFENSLNNDIKDILQLSFDGLEGGVKDIFLDISCLFVGEKYNNCAK 472

Query: 241 KMLSACHSNVDFGIMILMDLSLIMIESDRVQMHGLIQQMGRSLVRNESSQPGKRSRLWLV 300
           KMLSACH NVDFGIMILMDLSL+ IE DRVQMHGLIQQMG S+V NES + GKRSRLW  
Sbjct: 473 KMLSACHLNVDFGIMILMDLSLVTIEKDRVQMHGLIQQMGHSIVHNESFESGKRSRLWSE 532

Query: 301 EDIGEVFVNKSVRNSLPKGTDAIKAIKSDLPNP 334
            DI  VFVN S       GTDAIKAIK DLPNP
Sbjct: 533 RDIWNVFVNNS-------GTDAIKAIKLDLPNP 558

BLAST of CSPI01G16570.1 vs. TrEMBL
Match: E5GB33_CUCME (TIR-NBS-LRR disease resistance protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 1.8e-119
Identity = 233/335 (69.55%), Postives = 266/335 (79.40%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERCYFLRDVRREASKQY-GLVQLQETLLCEILKEDL 60
           +YGIGG GKTTLAK LYNKIA+QFE C FL +V REASKQ+ GL QLQE+LL EIL  DL
Sbjct: 233 IYGIGGIGKTTLAKALYNKIASQFEGCCFLSNV-REASKQFNGLAQLQESLLYEILMVDL 292

Query: 61  KVVNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTRNGHLL 120
           KVVN D+GINIIR+RLC KKVLIVLDDVD  EQLEA VG  DWFG+GS+II+ TRN HLL
Sbjct: 293 KVVNLDRGINIIRNRLCSKKVLIVLDDVDKLEQLEALVGGCDWFGKGSRIIVTTRNKHLL 352

Query: 121 SSHGFDEKHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKGLSVALVVLGS 180
            SHGFDE H I  L++D A+ LFSWHAFKK+ PSS+Y+ LS+ AT+YCKG  +ALVVLGS
Sbjct: 353 FSHGFDEIHNILGLNEDKAIELFSWHAFKKNRPSSNYLDLSKRATSYCKGHPLALVVLGS 412

Query: 181 FLRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGEDYNCAL 240
           FL  RDQ  W  ILDEFENSL KDIKD+LQLSFDGLEDK  DIFLDISCL VGE     +
Sbjct: 413 FLCIRDQAEWCSILDEFENSLNKDIKDILQLSFDGLEDKIKDIFLDISCLLVGEKVE-YV 472

Query: 241 KKMLSACHSNVDFGIMILMDLSLIMIESDRVQMHGLIQQMGRSLVRNESSQPGKRSRLWL 300
           K ML ACH N+DFG+++LMDLSLI IE+D+VQMH LI+QMG+ +V  ES + GKRSRLWL
Sbjct: 473 KDMLGACHVNLDFGVIVLMDLSLITIENDKVQMHDLIKQMGQKIVCGESLELGKRSRLWL 532

Query: 301 VEDIGEVFVNKSVRNSLPKGTDAIKAIKSDLPNPT 335
           V+D+ EV VN S       GTDAIKAIK D PNPT
Sbjct: 533 VQDVWEVLVNNS-------GTDAIKAIKLDFPNPT 558

BLAST of CSPI01G16570.1 vs. TrEMBL
Match: A0A0A0LLK3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G020890 PE=4 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 3.6e-115
Identity = 223/335 (66.57%), Postives = 263/335 (78.51%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERCYFLRDVRREASKQY-GLVQLQETLLCEILKEDL 60
           +YGIGG GKTTLAK LYNKIA QFE   FL +V RE SKQ+ GLVQLQE LL EILK DL
Sbjct: 224 IYGIGGIGKTTLAKALYNKIANQFEGFCFLSNV-RETSKQFNGLVQLQEKLLYEILKFDL 283

Query: 61  KVVNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTRNGHLL 120
           K+ N D+GINIIRSRL  KKVLIVLDDVD  +QLEA VGE DWFG GSKII+ TRN HLL
Sbjct: 284 KIGNLDEGINIIRSRLRSKKVLIVLDDVDKLKQLEALVGERDWFGHGSKIIVTTRNSHLL 343

Query: 121 SSHGFDEKHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKGLSVALVVLGS 180
           SSH FDEK+ + EL   H+L LFSWHAFKKSHPSS+Y+ LS+ ATNYCKG  +ALVVLGS
Sbjct: 344 SSHEFDEKYGVRELSHGHSLELFSWHAFKKSHPSSNYLDLSKRATNYCKGHPLALVVLGS 403

Query: 181 FLRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGEDYNCAL 240
           FL  RDQ +W  ILDEFENSL +DI+ ++Q+SFDGLE+K  +IFLDISCLFVGE  N  +
Sbjct: 404 FLCTRDQIKWRTILDEFENSLSEDIEHIIQISFDGLEEKIKEIFLDISCLFVGEKVN-YV 463

Query: 241 KKMLSACHSNVDFGIMILMDLSLIMIESDRVQMHGLIQQMGRSLVRNESSQPGKRSRLWL 300
           K +L+ CH ++DFGI++LMDLSLI +E++ VQMH LI+QMG+ +V  ES +PGKRSRLWL
Sbjct: 464 KSVLNTCHFSLDFGIIVLMDLSLITVENEEVQMHDLIRQMGQKIVNGESFEPGKRSRLWL 523

Query: 301 VEDIGEVFVNKSVRNSLPKGTDAIKAIKSDLPNPT 335
           V D+ +VF + S       GT A+KAIK DL NPT
Sbjct: 524 VHDVLKVFADNS-------GTIAVKAIKLDLSNPT 549

BLAST of CSPI01G16570.1 vs. TrEMBL
Match: E5GB36_CUCME (TIR-LRR-NBS disease resistance protein (Fragment) OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 2.3e-106
Identity = 217/334 (64.97%), Postives = 238/334 (71.26%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERCYFLRDVRREASKQYGLVQLQETLLCEILKEDLK 60
           MYGIGG GKTTLAK LYNKIATQFE   FL DVRREASK +GL+QLQ+TLL EILKEDLK
Sbjct: 92  MYGIGGIGKTTLAKALYNKIATQFEGSCFLLDVRREASK-HGLIQLQKTLLNEILKEDLK 151

Query: 61  VVNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTRNGHLLS 120
           VVNCDKGINIIRSRLC KKVLIVLDDVDH +QLEA VGE DWF +GSKII+ TRN HLLS
Sbjct: 152 VVNCDKGINIIRSRLCSKKVLIVLDDVDHRDQLEALVGERDWFCQGSKIIVTTRNKHLLS 211

Query: 121 SHGFDEKHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKGLSVALVVLGSF 180
           SHGFDE H I  L++D A+ LFSWHAFKK+HPSS+Y  LSE  T+YCKG  +ALVVLGSF
Sbjct: 212 SHGFDEIHNILGLNEDKAIELFSWHAFKKNHPSSNYFDLSERVTSYCKGHPLALVVLGSF 271

Query: 181 LRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGEDYNCALK 240
           L  RDQ  W  ILDEFENSL KDIKD+LQLSFDGLEDK  DIFLDISCL VGE     +K
Sbjct: 272 LCNRDQVEWCSILDEFENSLNKDIKDILQLSFDGLEDKVKDIFLDISCLLVGEKVEY-VK 331

Query: 241 KMLSACHSNVDFGIMILMDLSLIMIESDRVQMHGLIQQMGRSLVRNESSQPGKRSRLWLV 300
             LSACH                               MG  +V  ES + GKRSRLWL 
Sbjct: 332 DTLSACH-------------------------------MGHKIVCGESLELGKRSRLWLE 385

Query: 301 EDIGEVFVNKSVRNSLPKGTDAIKAIKSDLPNPT 335
           +D+ EVF + S       GT AIKAIK +  NPT
Sbjct: 392 KDVLEVFSSNS-------GTSAIKAIKLEFHNPT 385

BLAST of CSPI01G16570.1 vs. TAIR10
Match: AT1G27170.1 (AT1G27170.1 transmembrane receptors;ATP binding)

HSP 1 Score: 216.9 bits (551), Expect = 2.0e-56
Identity = 125/337 (37.09%), Postives = 205/337 (60.83%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERCYFLRDVRREASKQYGLVQLQETLLCEILKEDLK 60
           +YG+GG GKTTLAK  YNKI   FE+  F+ D+R  +S + GLV LQ+TL+ E+ +   +
Sbjct: 216 LYGMGGIGKTTLAKAFYNKIVGNFEQRAFISDIRERSSAENGLVTLQKTLIKELFRLVPE 275

Query: 61  VVNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTRNGHLLS 120
           + +   G+  I++ +  KK+++VLDDVDH +Q+ A VGE  W+G+G+ I++ TR+  +LS
Sbjct: 276 IEDVSIGLEKIKANVHEKKIIVVLDDVDHIDQVHALVGETRWYGQGTLIVITTRDSEILS 335

Query: 121 SHGFDEKHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKGLSVALVVLGSF 180
               ++++++  L +  AL LFS+H+ +K  P+ + + LS+        L +A+ V GS 
Sbjct: 336 KLSVNQQYEVKCLTEPQALKLFSYHSLRKEEPTKNLLALSKKIVQISGLLPLAVEVFGSL 395

Query: 181 LRGRDQTR-WSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGEDYNCAL 240
           L  + + + W   LD+ + + P +++DVL+LSF  L+D+   +FLDI+CLF+       +
Sbjct: 396 LYDKKEEKDWQTQLDKLKKTQPGNLQDVLELSFKSLDDEEKKVFLDIACLFL----KMEI 455

Query: 241 KK-----MLSACHSNVDFGIMILMDLSLI-MIESDRVQMHGLIQQMGRSLVRNESSQ-PG 300
           KK     +L  C  N +  + +L   SL+ ++ +D + MH  I+ MGR +V  ES + PG
Sbjct: 456 KKDEVVIVLKGCGLNAEAALSVLRQKSLVKILANDTLWMHDQIRDMGRQMVLKESREDPG 515

Query: 301 KRSRLWLVEDIGEVFVNKSVRNSLPKGTDAIKAIKSD 330
            RSRLW   D GE+    +V N++ KGT +I+ I  D
Sbjct: 516 LRSRLW---DRGEIM---TVLNNM-KGTSSIRGIVLD 541

BLAST of CSPI01G16570.1 vs. TAIR10
Match: AT5G36930.2 (AT5G36930.2 Disease resistance protein (TIR-NBS-LRR class) family)

HSP 1 Score: 210.7 bits (535), Expect = 1.4e-54
Identity = 128/334 (38.32%), Postives = 188/334 (56.29%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERCYFLRDVRREASKQYGLVQLQETLLCEILKE-DL 60
           +YG+GG GKTTLAK  +N+ +  FE   FL + R  + K  G   LQ  LL +IL+  D+
Sbjct: 217 IYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENFREYSKKPEGRTHLQHQLLSDILRRNDI 276

Query: 61  KVVNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTRNGHLL 120
           +    D   + ++ R   K+VL+V+DDVD   QL +   + D FG GS+II+ TRN HLL
Sbjct: 277 EFKGLD---HAVKERFRSKRVLLVVDDVDDVHQLNSAAIDRDCFGHGSRIIITTRNMHLL 336

Query: 121 SSHGFDEKHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKGLSVALVVLGS 180
                +  +   ELD D +L LFSWHAF+ S P   ++  SE    YC GL +A+ VLG+
Sbjct: 337 KQLRAEGSYSPKELDGDESLELFSWHAFRTSEPPKEFLQHSEEVVTYCAGLPLAVEVLGA 396

Query: 181 FLRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGEDYNCAL 240
           FL  R    W   L   +     +I+  LQ+SF+ L  +  D+FLDI+C F+G D +  +
Sbjct: 397 FLIERSIREWESTLKLLKRIPNDNIQAKLQISFNALTIEQKDVFLDIACFFIGVD-SYYV 456

Query: 241 KKMLSACHSNVDFGIMILMDLSLIMIESDRVQMHGLIQQMGRSLVRNESSQP-GKRSRLW 300
             +L  C+   D  + +LM+  LI I  + + MH L++ MGR +VR  S +  G+RSRLW
Sbjct: 457 ACILDGCNLYPDIVLSLLMERCLITISGNNIMMHDLLRDMGRQIVREISPKKCGERSRLW 516

Query: 301 LVEDIGEVFVNKSVRNSLPKGTDAIK--AIKSDL 331
              D+  V   KS       GT+AI+  ++K+D+
Sbjct: 517 SHNDVVGVLKKKS-------GTNAIEGLSLKADV 539

BLAST of CSPI01G16570.1 vs. TAIR10
Match: AT5G17680.1 (AT5G17680.1 disease resistance protein (TIR-NBS-LRR class), putative)

HSP 1 Score: 206.8 bits (525), Expect = 2.0e-53
Identity = 131/333 (39.34%), Postives = 192/333 (57.66%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERCYFLRDVRREASKQYGLVQLQETLLCEILKE-DL 60
           ++G+GG GKTT+AK LYN+++ QF+   F+ +V+ E   +YG+ +LQ   LC + +E D 
Sbjct: 211 IWGMGGVGKTTIAKYLYNQLSGQFQVHCFMENVK-EVCNRYGVRRLQVEFLCRMFQERDK 270

Query: 61  KVVNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTRNGHLL 120
           +  +     NII+ R   K V IVLDDVD  EQL   V E  WFG GS+II+ TR+ HLL
Sbjct: 271 EAWSSVSCCNIIKERFRHKMVFIVLDDVDRSEQLNELVKETGWFGPGSRIIVTTRDRHLL 330

Query: 121 SSHGFDEKHKIHELDQDHALVLFSWHAFKKS-HPSSSYIGLSESATNYCKGLSVALVVLG 180
            SHG +  +K+  L +  AL LF  +AF++       +  LS  A NY  GL +AL VLG
Sbjct: 331 LSHGINLVYKVKCLPKKEALQLFCNYAFREEIILPHGFEELSVQAVNYASGLPLALRVLG 390

Query: 181 SFLRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGEDYNCA 240
           SFL  R Q  W   L   +     DI +VL++S+DGL+++   IFL ISC +  +  +  
Sbjct: 391 SFLYRRSQIEWESTLARLKTYPHSDIMEVLRVSYDGLDEQEKAIFLYISCFYNMKQVD-Y 450

Query: 241 LKKMLSACHSNVDFGIMILMDLSLIMIESDRVQMHGLIQQMGRSLVRNES-SQPGKRSRL 300
           ++K+L  C    + GI IL + SLI+  +  V++H L++QMGR LVR ++ + P +R  L
Sbjct: 451 VRKLLDLCGYAAEIGITILTEKSLIVESNGCVKIHDLLEQMGRELVRQQAVNNPAQRLLL 510

Query: 301 WLVEDIGEVFVNKSVRNSLPKGTDAIKAIKSDL 331
           W  EDI  +    S       GT  ++ I  +L
Sbjct: 511 WDPEDICHLLSENS-------GTQLVEGISLNL 534

BLAST of CSPI01G16570.1 vs. TAIR10
Match: AT4G11170.1 (AT4G11170.1 Disease resistance protein (TIR-NBS-LRR class) family)

HSP 1 Score: 198.4 bits (503), Expect = 7.2e-51
Identity = 120/338 (35.50%), Postives = 192/338 (56.80%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERCYFLRDVRREASK----QYGL-VQLQETLLCEIL 60
           ++G  G GKTT+A+ LYN+    F    F+ +VR    +     YGL + LQ+  L ++L
Sbjct: 210 IWGPAGVGKTTIARALYNQYHENFNLSIFMENVRESYGEAGLDDYGLKLHLQQRFLSKLL 269

Query: 61  -KEDLKVVNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTR 120
            ++DL+V    + +  I  RL  +KVLI+LDDVD+ EQL+A   E+ WFG  S+I++ T+
Sbjct: 270 DQKDLRV----RHLGAIEERLKSQKVLIILDDVDNIEQLKALAKENQWFGNKSRIVVTTQ 329

Query: 121 NGHLLSSHGFDEKHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKGLSVAL 180
           N  LL SH  +  +++    +  AL +F  HAFK+S PS     L+   T     L +AL
Sbjct: 330 NKQLLVSHDINHMYQVAYPSKQEALTIFCQHAFKQSSPSDDLKHLAIEFTTLAGHLPLAL 389

Query: 181 VVLGSFLRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGED 240
            VLGSF+RG+ +  W   L   ++ L  +++ VL++ +DGL D   D+FL I+C+F G+ 
Sbjct: 390 RVLGSFMRGKGKEEWEFSLPTLKSRLDGEVEKVLKVGYDGLHDHEKDLFLHIACIFSGQH 449

Query: 241 YNCALKKMLSACHSNVDFGIMILMDLSLIM-IESDRVQMHGLIQQMGRSLVRNES-SQPG 300
            N   + +++   + V FG+ +L D SLI   E+ R++MH L++Q+G+ +VR +S  +PG
Sbjct: 450 ENYLKQMIIANNDTYVSFGLQVLADKSLIQKFENGRIEMHSLLRQLGKEVVRKQSIYEPG 509

Query: 301 KRSRLWLVEDIGEVFVNKSVRNSLPKGTDAIKAIKSDL 331
           KR  L   ++   V  N +       GT  +  I  D+
Sbjct: 510 KRQFLMNAKETCGVLSNNT-------GTGTVLGISLDM 536

BLAST of CSPI01G16570.1 vs. TAIR10
Match: AT1G27180.1 (AT1G27180.1 disease resistance protein (TIR-NBS-LRR class), putative)

HSP 1 Score: 194.5 bits (493), Expect = 1.0e-49
Identity = 122/334 (36.53%), Postives = 192/334 (57.49%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERC-YFLRDVRREASKQYGLVQLQETLLCEILKEDL 60
           +YG+GG GKTTLAK  YNKI   F R   F+  VR ++S Q GLV LQ+TL+ E+ +   
Sbjct: 389 LYGMGGIGKTTLAKAFYNKIIVNFNRHRVFIESVRGKSSDQDGLVNLQKTLIKELFRLVP 448

Query: 61  KVVNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTRNGHLL 120
           ++ +   G+  I+  +  KK+++VLDDVDH +Q+ A VGE  W+G GS I++ TR+  +L
Sbjct: 449 EIEDVSIGLEKIKENVHEKKIIVVLDDVDHIDQVNALVGETSWYGEGSLIVITTRDSEIL 508

Query: 121 SSHGFDEKHKIHELDQDHALVLFSWHAFKKSH-PSSSYIGLSESATNYCKGLSVALVVLG 180
           S    ++++++  L +  AL LFS+++ +K   P+   + LS+        L +A+ V G
Sbjct: 509 SKLSVNQQYEVKCLTEPQALKLFSFYSLRKEKPPTQGLLELSKKIAEVTGLLPLAVKVFG 568

Query: 181 SFLRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGEDYNC- 240
           S    +D+  W   L++ +    K +  VL LSF  L+++   IFLDI+CLF+  D    
Sbjct: 569 SHFYDKDENEWQVELEKLKTQQDK-LHGVLALSFKSLDEEEKKIFLDIACLFLKMDITKE 628

Query: 241 ALKKMLSACHSNVDFGIMILMDLSLI-MIESDRVQMHGLIQQMGRSLVRNESS-QPGKRS 300
            +  +L  C  N +  + +L+  SL+ ++  D + MH  I+ MGR +V  ESS  P  RS
Sbjct: 629 EVVDILKGCGLNAEAALRVLIQKSLLTILTDDTLWMHDQIRDMGRQMVHKESSDDPEMRS 688

Query: 301 RLWLVEDIGEVFVNKSVRNSLPKGTDAIKAIKSD 330
           RLW   D GE+    +V + + KGT +I+ I  D
Sbjct: 689 RLW---DRGEIM---NVLDYM-KGTSSIRGIVLD 714

BLAST of CSPI01G16570.1 vs. NCBI nr
Match: gi|659070449|ref|XP_008455151.1| (PREDICTED: TMV resistance protein N-like isoform X1 [Cucumis melo])

HSP 1 Score: 524.2 bits (1349), Expect = 1.6e-145
Identity = 271/335 (80.90%), Postives = 293/335 (87.46%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERCYFLRDVRREASKQYGLVQLQETLLCEILKEDLK 60
           MYGIGG GKTTLAK LYNKIA QFE C FL+DVRREASK +GLV+LQETLL +ILKEDLK
Sbjct: 257 MYGIGGIGKTTLAKALYNKIANQFEGCCFLQDVRREASK-HGLVKLQETLLNDILKEDLK 316

Query: 61  VVNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTRNGHLLS 120
           VV+ D+GINIIRSRLC KKVLIVLDDVD  EQLEA VG  DWFGRGSKII+ TRN HLL 
Sbjct: 317 VVSRDRGINIIRSRLCSKKVLIVLDDVDDREQLEALVGGRDWFGRGSKIIVTTRNEHLLF 376

Query: 121 SHGFDE-KHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKGLSVALVVLGS 180
           SHGFD+ KHKI EL+QDHAL LFSWHAFKKSHPSS+Y+GLSE ATNYCKGLS+ALVVLGS
Sbjct: 377 SHGFDDQKHKIQELNQDHALELFSWHAFKKSHPSSNYLGLSERATNYCKGLSLALVVLGS 436

Query: 181 FLRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGEDYNCAL 240
           FLRGRDQ  W+CILDEFE SL KDIKDVLQLSFDGLEDKA DIFLDISCL VGE+YNCA 
Sbjct: 437 FLRGRDQAEWNCILDEFETSLRKDIKDVLQLSFDGLEDKAKDIFLDISCLLVGEEYNCA- 496

Query: 241 KKMLSACHSNVDFGIMILMDLSLIMIESDRVQMHGLIQQMGRSLVRNESSQPGKRSRLWL 300
           KKMLSACH N+DFGIMIL+DLSL+ IE+DRVQMH LIQQMGRS+V NESS+PGKRSRLWL
Sbjct: 497 KKMLSACHLNIDFGIMILVDLSLVTIETDRVQMHELIQQMGRSIVHNESSEPGKRSRLWL 556

Query: 301 VEDIGEVFVNKSVRNSLPKGTDAIKAIKSDLPNPT 335
           V+DI EVFVN S       GTDA+KAIK DLPNPT
Sbjct: 557 VQDIWEVFVNNS-------GTDAVKAIKLDLPNPT 582

BLAST of CSPI01G16570.1 vs. NCBI nr
Match: gi|659070451|ref|XP_008455159.1| (PREDICTED: TMV resistance protein N-like isoform X2 [Cucumis melo])

HSP 1 Score: 524.2 bits (1349), Expect = 1.6e-145
Identity = 271/335 (80.90%), Postives = 293/335 (87.46%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERCYFLRDVRREASKQYGLVQLQETLLCEILKEDLK 60
           MYGIGG GKTTLAK LYNKIA QFE C FL+DVRREASK +GLV+LQETLL +ILKEDLK
Sbjct: 70  MYGIGGIGKTTLAKALYNKIANQFEGCCFLQDVRREASK-HGLVKLQETLLNDILKEDLK 129

Query: 61  VVNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTRNGHLLS 120
           VV+ D+GINIIRSRLC KKVLIVLDDVD  EQLEA VG  DWFGRGSKII+ TRN HLL 
Sbjct: 130 VVSRDRGINIIRSRLCSKKVLIVLDDVDDREQLEALVGGRDWFGRGSKIIVTTRNEHLLF 189

Query: 121 SHGFDE-KHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKGLSVALVVLGS 180
           SHGFD+ KHKI EL+QDHAL LFSWHAFKKSHPSS+Y+GLSE ATNYCKGLS+ALVVLGS
Sbjct: 190 SHGFDDQKHKIQELNQDHALELFSWHAFKKSHPSSNYLGLSERATNYCKGLSLALVVLGS 249

Query: 181 FLRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGEDYNCAL 240
           FLRGRDQ  W+CILDEFE SL KDIKDVLQLSFDGLEDKA DIFLDISCL VGE+YNCA 
Sbjct: 250 FLRGRDQAEWNCILDEFETSLRKDIKDVLQLSFDGLEDKAKDIFLDISCLLVGEEYNCA- 309

Query: 241 KKMLSACHSNVDFGIMILMDLSLIMIESDRVQMHGLIQQMGRSLVRNESSQPGKRSRLWL 300
           KKMLSACH N+DFGIMIL+DLSL+ IE+DRVQMH LIQQMGRS+V NESS+PGKRSRLWL
Sbjct: 310 KKMLSACHLNIDFGIMILVDLSLVTIETDRVQMHELIQQMGRSIVHNESSEPGKRSRLWL 369

Query: 301 VEDIGEVFVNKSVRNSLPKGTDAIKAIKSDLPNPT 335
           V+DI EVFVN S       GTDA+KAIK DLPNPT
Sbjct: 370 VQDIWEVFVNNS-------GTDAVKAIKLDLPNPT 395

BLAST of CSPI01G16570.1 vs. NCBI nr
Match: gi|778674008|ref|XP_011650108.1| (PREDICTED: TMV resistance protein N-like [Cucumis sativus])

HSP 1 Score: 497.7 bits (1280), Expect = 1.6e-137
Identity = 262/335 (78.21%), Postives = 285/335 (85.07%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERCYFLRDVRREASKQYGLVQLQETLLCEILKEDLK 60
           +YGIGG GKTTLAK LYNKIATQFE C FL+DVRREASK +GLVQLQETLL EILKEDLK
Sbjct: 182 LYGIGGIGKTTLAKALYNKIATQFEGCCFLQDVRREASK-HGLVQLQETLLNEILKEDLK 241

Query: 61  V-VNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTRNGHLL 120
           V V+ D+GINIIRSRLC KKVLIVLDDV+  EQLEA VG  DWFG+GSKII+ TRN HLL
Sbjct: 242 VIVSRDRGINIIRSRLCSKKVLIVLDDVNDLEQLEALVGGRDWFGQGSKIIVTTRNEHLL 301

Query: 121 SSHGFDEKHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKGLSVALVVLGS 180
           SSHGFDEKHKI EL+QDHAL LFSWHAFKKSHPSS+Y+  S+ AT+YCKGLS+ALVVLGS
Sbjct: 302 SSHGFDEKHKIQELNQDHALELFSWHAFKKSHPSSNYLDFSKRATSYCKGLSLALVVLGS 361

Query: 181 FLRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGEDYNCAL 240
           FL GR +  W+ +LDEFENSL KDIKDVLQLSFDGLEDK  DIFLDISCLFVGE+Y CA 
Sbjct: 362 FLCGRAKEEWNGMLDEFENSLRKDIKDVLQLSFDGLEDKIKDIFLDISCLFVGEEYKCA- 421

Query: 241 KKMLSACHSNVDFGIMILMDLSLIMIESDRVQMHGLIQQMGRSLVRNESSQPGKRSRLWL 300
           KKMLSACH N+DFGIMILMDLSLI +E DRVQMH LIQQMGRS+V NESS+PGKRSRLWL
Sbjct: 422 KKMLSACHLNIDFGIMILMDLSLITVEMDRVQMHELIQQMGRSIVHNESSEPGKRSRLWL 481

Query: 301 VEDIGEVFVNKSVRNSLPKGTDAIKAIKSDLPNPT 335
             DI EVFVN S       GTDA+KAIK DLP  T
Sbjct: 482 EHDIWEVFVNNS-------GTDAVKAIKLDLPKST 507

BLAST of CSPI01G16570.1 vs. NCBI nr
Match: gi|700205791|gb|KGN60910.1| (hypothetical protein Csa_2G022790 [Cucumis sativus])

HSP 1 Score: 497.7 bits (1280), Expect = 1.6e-137
Identity = 262/335 (78.21%), Postives = 285/335 (85.07%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERCYFLRDVRREASKQYGLVQLQETLLCEILKEDLK 60
           +YGIGG GKTTLAK LYNKIATQFE C FL+DVRREASK +GLVQLQETLL EILKEDLK
Sbjct: 223 LYGIGGIGKTTLAKALYNKIATQFEGCCFLQDVRREASK-HGLVQLQETLLNEILKEDLK 282

Query: 61  V-VNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTRNGHLL 120
           V V+ D+GINIIRSRLC KKVLIVLDDV+  EQLEA VG  DWFG+GSKII+ TRN HLL
Sbjct: 283 VIVSRDRGINIIRSRLCSKKVLIVLDDVNDLEQLEALVGGRDWFGQGSKIIVTTRNEHLL 342

Query: 121 SSHGFDEKHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKGLSVALVVLGS 180
           SSHGFDEKHKI EL+QDHAL LFSWHAFKKSHPSS+Y+  S+ AT+YCKGLS+ALVVLGS
Sbjct: 343 SSHGFDEKHKIQELNQDHALELFSWHAFKKSHPSSNYLDFSKRATSYCKGLSLALVVLGS 402

Query: 181 FLRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGEDYNCAL 240
           FL GR +  W+ +LDEFENSL KDIKDVLQLSFDGLEDK  DIFLDISCLFVGE+Y CA 
Sbjct: 403 FLCGRAKEEWNGMLDEFENSLRKDIKDVLQLSFDGLEDKIKDIFLDISCLFVGEEYKCA- 462

Query: 241 KKMLSACHSNVDFGIMILMDLSLIMIESDRVQMHGLIQQMGRSLVRNESSQPGKRSRLWL 300
           KKMLSACH N+DFGIMILMDLSLI +E DRVQMH LIQQMGRS+V NESS+PGKRSRLWL
Sbjct: 463 KKMLSACHLNIDFGIMILMDLSLITVEMDRVQMHELIQQMGRSIVHNESSEPGKRSRLWL 522

Query: 301 VEDIGEVFVNKSVRNSLPKGTDAIKAIKSDLPNPT 335
             DI EVFVN S       GTDA+KAIK DLP  T
Sbjct: 523 EHDIWEVFVNNS-------GTDAVKAIKLDLPKST 548

BLAST of CSPI01G16570.1 vs. NCBI nr
Match: gi|778666722|ref|XP_011648798.1| (PREDICTED: TMV resistance protein N-like [Cucumis sativus])

HSP 1 Score: 457.2 bits (1175), Expect = 2.4e-125
Identity = 238/333 (71.47%), Postives = 264/333 (79.28%), Query Frame = 1

Query: 1   MYGIGGTGKTTLAKDLYNKIATQFERCYFLRDVRREASKQYGLVQLQETLLCEILKEDLK 60
           +YGIGG GKTTLAK LYNKIA+QFE C FL +VR+ +++  GLVQLQ+ LL EIL++DLK
Sbjct: 233 IYGIGGLGKTTLAKALYNKIASQFEGCCFLSNVRQASNQFNGLVQLQQNLLYEILEDDLK 292

Query: 61  VVNCDKGINIIRSRLCLKKVLIVLDDVDHHEQLEAPVGEHDWFGRGSKIIMLTRNGHLLS 120
            VN DKGI IIR+RL  KKVLIVLDDVD  EQLEA VG  DWFG+GSKII+ TRN HLLS
Sbjct: 293 FVNLDKGITIIRNRLRSKKVLIVLDDVDKLEQLEALVGGRDWFGQGSKIIVTTRNSHLLS 352

Query: 121 SHGFDEKHKIHELDQDHALVLFSWHAFKKSHPSSSYIGLSESATNYCKGLSVALVVLGSF 180
           SHGFDE H I  L+QD A+ LFSWHAFK+SHPSS+Y+ L+E AT+YCKG  +ALVVLGSF
Sbjct: 353 SHGFDEMHNIQGLNQDRAIELFSWHAFKESHPSSNYLDLAERATSYCKGHPLALVVLGSF 412

Query: 181 LRGRDQTRWSCILDEFENSLPKDIKDVLQLSFDGLEDKANDIFLDISCLFVGEDYNCALK 240
           L  R QT W  ILD+FENSL  DIKD+LQLSFDGLE    DIFLDISCLFVGE YN   K
Sbjct: 413 LCNRGQTEWRSILDKFENSLNNDIKDILQLSFDGLEGGVKDIFLDISCLFVGEKYNNCAK 472

Query: 241 KMLSACHSNVDFGIMILMDLSLIMIESDRVQMHGLIQQMGRSLVRNESSQPGKRSRLWLV 300
           KMLSACH NVDFGIMILMDLSL+ IE DRVQMHGLIQQMG S+V NES + GKRSRLW  
Sbjct: 473 KMLSACHLNVDFGIMILMDLSLVTIEKDRVQMHGLIQQMGHSIVHNESFESGKRSRLWSE 532

Query: 301 EDIGEVFVNKSVRNSLPKGTDAIKAIKSDLPNP 334
            DI  VFVN S       GTDAIKAIK DLPNP
Sbjct: 533 RDIWNVFVNNS-------GTDAIKAIKLDLPNP 558

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TMVRN_NICGU4.1e-5639.22TMV resistance protein N OS=Nicotiana glutinosa GN=N PE=1 SV=1[more]
Y4117_ARATH1.3e-4935.50Putative disease resistance protein At4g11170 OS=Arabidopsis thaliana GN=At4g111... [more]
TAO1_ARATH1.4e-4033.23Disease resistance protein TAO1 OS=Arabidopsis thaliana GN=TAO1 PE=3 SV=1[more]
RLM1A_ARATH1.6e-3932.83Disease resistance protein RML1A OS=Arabidopsis thaliana GN=RLM1A PE=3 SV=1[more]
RPS6R_ARATH4.6e-3931.87Disease resistance protein RPS6 OS=Arabidopsis thaliana GN=RPS6 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LFS4_CUCSA1.1e-13778.21Uncharacterized protein OS=Cucumis sativus GN=Csa_2G022790 PE=4 SV=1[more]
A0A0A0LLL0_CUCSA1.7e-12571.47Uncharacterized protein OS=Cucumis sativus GN=Csa_2G020940 PE=4 SV=1[more]
E5GB33_CUCME1.8e-11969.55TIR-NBS-LRR disease resistance protein OS=Cucumis melo subsp. melo PE=4 SV=1[more]
A0A0A0LLK3_CUCSA3.6e-11566.57Uncharacterized protein OS=Cucumis sativus GN=Csa_2G020890 PE=4 SV=1[more]
E5GB36_CUCME2.3e-10664.97TIR-LRR-NBS disease resistance protein (Fragment) OS=Cucumis melo subsp. melo PE... [more]
Match NameE-valueIdentityDescription
AT1G27170.12.0e-5637.09 transmembrane receptors;ATP binding[more]
AT5G36930.21.4e-5438.32 Disease resistance protein (TIR-NBS-LRR class) family[more]
AT5G17680.12.0e-5339.34 disease resistance protein (TIR-NBS-LRR class), putative[more]
AT4G11170.17.2e-5135.50 Disease resistance protein (TIR-NBS-LRR class) family[more]
AT1G27180.11.0e-4936.53 disease resistance protein (TIR-NBS-LRR class), putative[more]
Match NameE-valueIdentityDescription
gi|659070449|ref|XP_008455151.1|1.6e-14580.90PREDICTED: TMV resistance protein N-like isoform X1 [Cucumis melo][more]
gi|659070451|ref|XP_008455159.1|1.6e-14580.90PREDICTED: TMV resistance protein N-like isoform X2 [Cucumis melo][more]
gi|778674008|ref|XP_011650108.1|1.6e-13778.21PREDICTED: TMV resistance protein N-like [Cucumis sativus][more]
gi|700205791|gb|KGN60910.1|1.6e-13778.21hypothetical protein Csa_2G022790 [Cucumis sativus][more]
gi|778666722|ref|XP_011648798.1|2.4e-12571.47PREDICTED: TMV resistance protein N-like [Cucumis sativus][more]
The following terms have been associated with this mRNA:
Vocabulary: INTERPRO
TermDefinition
IPR002182NB-ARC
IPR011991Winged helix-turn-helix DNA-binding domain
IPR027417P-loop_NTPase
Vocabulary: Molecular Function
TermDefinition
GO:0043531ADP binding
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007165 signal transduction
cellular_component GO:0005575 cellular_component
molecular_function GO:0043531 ADP binding
molecular_function GO:0005524 ATP binding

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI01G16570CSPI01G16570gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI01G16570.1CSPI01G16570.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI01G16570.1.cds1CSPI01G16570.1.cds1CDS
CSPI01G16570.1.cds2CSPI01G16570.1.cds2CDS


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 1..243
score: 1.7
IPR011991Winged helix-turn-helix DNA-binding domainunknownSSF46785"Winged helix" DNA-binding domaincoord: 203..306
score: 2.99
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3DG3DSA:3.40.50.300coord: 2..142
score: 3.
IPR027417P-loop containing nucleoside triphosphate hydrolaseunknownSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 2..226
score: 3.57
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 167..181
score: 1.9E-7coord: 74..88
score: 1.9E-7coord: 1..8
score: 1.
NoneNo IPR availablePANTHERPTHR11017LEUCINE-RICH REPEAT-CONTAINING PROTEINcoord: 2..326
score: 3.4E
NoneNo IPR availablePANTHERPTHR11017:SF169DISEASE RESISTANCE PROTEIN-RELATEDcoord: 2..326
score: 3.4E