CSPI04G03240 (gene) Wild cucumber (PI 183967)

NameCSPI04G03240
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionCysteine protease, putative
LocationChr4 : 2011549 .. 2012898 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCATGATGAAATTTCTTATTGTTTTCGTTGTTTTGATTGCTTTCACGTCTCATTTGTGTGAGAGCTTTGAGTTGGAAGGAAAGGATTTTGAATCTGAAAGAAGTCTCATGCAACTCTACAAAAGATGGAGTAGCCACCATAGAATCTCAAGAAATGCACATGAGATGCACAAACGTTTCAAGATCTTTCAAGATAATGCAAAACATGTGTTTAGAGTGAACCACATGGGAAAATCATTAAAATTGCGGCTTAACCAATTTGCTGATTTGTCTGATGATGAGTTTAGTATGATGTACGGTTCCAACATTACTCACTACAACGGCTTACATGCCAATCGTGTTGGTGAATTTATGTATGAACGAGCAATGAATATCCCATCTTCAATTGATTGGAGGCAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCCATTGTGGTAATTAATTTAATTTCATTTATGATCTTTCTAAAGCCTAATGCCTTCTAGCTAATAGTTACTAATGCAATTATGTGTTATGTGTTCGACAGGAAGTTGTTGGGCGTTTGCAGCTGTGGCCGCCGTGGAATCTATTCACCAAATAAAAACAAATGAATTAGTATCTCTATCAGAGCAAGAAGTGGTGGATTGTGACTATAAAGTCGGTGGTTGTCGTGGAGGAAATTATAACTCTGCATTTGAGTTCATAATGCAAAATGGTGGAATCACAATTGAGGAAAACTATCCGTATTTTGCCGGAAACGGATATTGTCGTAGACGAGGAGTGAGTTTAAACCAATATAATTATGAGTGCATTATTCATTATTGATCATAATATAATTATGTGTATTGTGTTTGATATTTCACTAATAGCCTAACAATGAGAGAGTAACAATTGATGGATATGAGCGCGTACCTCAAAACAACGAGTATGCTTTGATGAAAGCAGTGGCACACCAACCGGTAGCAGTGTCGGTAGCTTCAAGTGGGAGTGATTTTAGATTTTACGGGGAGGCAAGTGTAGTTAATTTTGTTGTTGTTAATTATAATTTGGTGCAACATATATAATTAAAAGAAAAAATGATAATTATTTGCAGGGAATGCTTAGAGAAGATAGCTTTTGCGGATATAGAATTGACCACACGGTAGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTACATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCAATGCAACCTTCCTTTCCCGTCAAGTATTGATTGGATTTCCTCTTTTTTATTTTAGAACGGGTAGCTAGCTAGCTAAGCTAG

mRNA sequence

ATGACCATGATGAAATTTCTTATTGTTTTCGTTGTTTTGATTGCTTTCACGTCTCATTTGTGTGAGAGCTTTGAGTTGGAAGGAAAGGATTTTGAATCTGAAAGAAGTCTCATGCAACTCTACAAAAGATGGAGTAGCCACCATAGAATCTCAAGAAATGCACATGAGATGCACAAACGTTTCAAGATCTTTCAAGATAATGCAAAACATGTGTTTAGAGTGAACCACATGGGAAAATCATTAAAATTGCGGCTTAACCAATTTGCTGATTTGTCTGATGATGAGTTTAGTATGATGTACGGTTCCAACATTACTCACTACAACGGCTTACATGCCAATCGTGTTGGTGAATTTATGTATGAACGAGCAATGAATATCCCATCTTCAATTGATTGGAGGCAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCCATTGTGGAAGTTGTTGGGCGTTTGCAGCTGTGGCCGCCGTGGAATCTATTCACCAAATAAAAACAAATGAATTAGTATCTCTATCAGAGCAAGAAGTGGTGGATTGTGACTATAAAGTCGGTGGTTGTCGTGGAGGAAATTATAACTCTGCATTTGAGTTCATAATGCAAAATGGTGGAATCACAATTGAGGAAAACTATCCGTATTTTGCCGGAAACGGATATTGTCGTAGACGAGGACCTAACAATGAGAGAGTAACAATTGATGGATATGAGCGCGTACCTCAAAACAACGAGTATGCTTTGATGAAAGCAGTGGCACACCAACCGGTAGCAGTGTCGGGAATGCTTAGAGAAGATAGCTTTTGCGGATATAGAATTGACCACACGGTAGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTACATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCAATGCAACCTTCCTTTCCCGTCAAGTATTGA

Coding sequence (CDS)

ATGACCATGATGAAATTTCTTATTGTTTTCGTTGTTTTGATTGCTTTCACGTCTCATTTGTGTGAGAGCTTTGAGTTGGAAGGAAAGGATTTTGAATCTGAAAGAAGTCTCATGCAACTCTACAAAAGATGGAGTAGCCACCATAGAATCTCAAGAAATGCACATGAGATGCACAAACGTTTCAAGATCTTTCAAGATAATGCAAAACATGTGTTTAGAGTGAACCACATGGGAAAATCATTAAAATTGCGGCTTAACCAATTTGCTGATTTGTCTGATGATGAGTTTAGTATGATGTACGGTTCCAACATTACTCACTACAACGGCTTACATGCCAATCGTGTTGGTGAATTTATGTATGAACGAGCAATGAATATCCCATCTTCAATTGATTGGAGGCAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCCATTGTGGAAGTTGTTGGGCGTTTGCAGCTGTGGCCGCCGTGGAATCTATTCACCAAATAAAAACAAATGAATTAGTATCTCTATCAGAGCAAGAAGTGGTGGATTGTGACTATAAAGTCGGTGGTTGTCGTGGAGGAAATTATAACTCTGCATTTGAGTTCATAATGCAAAATGGTGGAATCACAATTGAGGAAAACTATCCGTATTTTGCCGGAAACGGATATTGTCGTAGACGAGGACCTAACAATGAGAGAGTAACAATTGATGGATATGAGCGCGTACCTCAAAACAACGAGTATGCTTTGATGAAAGCAGTGGCACACCAACCGGTAGCAGTGTCGGGAATGCTTAGAGAAGATAGCTTTTGCGGATATAGAATTGACCACACGGTAGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTACATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCAATGCAACCTTCCTTTCCCGTCAAGTATTGA
BLAST of CSPI04G03240 vs. Swiss-Prot
Match: CYSEP_RICCO (Vignain OS=Ricinus communis GN=CYSEP PE=1 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 1.5e-87
Identity = 168/346 (48.55%), Postives = 228/346 (65.90%), Query Frame = 1

Query: 3   MMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M KF+++ + L A    + ESF+   K+ ESE SL  LY+RW SHH +SR+ HE  KRF 
Sbjct: 1   MQKFILLALSL-ALVLAITESFDFHEKELESEESLWGLYERWRSHHTVSRSLHEKQKRFN 60

Query: 63  IFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYN----GLHANRVGE 122
           +F+ NA HV   N M K  KL+LN+FAD+++ EF   Y GS + H+     G   N  G 
Sbjct: 61  VFKHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKHHRMFRGGPRGN--GT 120

Query: 123 FMYERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 182
           FMYE+   +P+S+DWR+KGAV ++K+QG CGSCWAF+ + AVE I+QIKTN+LVSLSEQE
Sbjct: 121 FMYEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQE 180

Query: 183 VVDCDYKVG-GCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGY 242
           +VDCD     GC GG  + AFEFI Q GGIT E NYPY A +G C     N   V+IDG+
Sbjct: 181 LVDCDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAVSIDGH 240

Query: 243 ERVPQNNEYALMKAVAHQPVAVSGMLREDSF-----------CGYRIDHTVVVVGYGSDE 302
           E VP+N+E AL+KAVA+QPV+V+       F           CG  +DH V +VGYG+  
Sbjct: 241 ENVPENDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGSCGTELDHGVAIVGYGTTI 300

Query: 303 EG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 331
           +G  YW ++N +G +WG  GY++M+RG  + +G+CG+AM+ S+P+K
Sbjct: 301 DGTKYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIAMEASYPIK 343

BLAST of CSPI04G03240 vs. Swiss-Prot
Match: CYSEP_VIGMU (Vignain OS=Vigna mungo PE=1 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 1.5e-87
Identity = 166/345 (48.12%), Postives = 225/345 (65.22%), Query Frame = 1

Query: 4   MKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFKI 63
           MK L+  V+ ++    +  SF+   KD ESE SL  LY+RW SHH +SR+  E HKRF +
Sbjct: 3   MKKLLWVVLSLSLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKHKRFNV 62

Query: 64  FQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNGLHANRVGE--FMY 123
           F+ N  HV   N M K  KL+LN+FAD+++ EF   Y GS + H+     ++ G   FMY
Sbjct: 63  FKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQHGSGTFMY 122

Query: 124 ERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVD 183
           E+  ++P+S+DWR+KGAV  +K+QG CGSCWAF+ + AVE I+QIKTN+LVSLSEQE+VD
Sbjct: 123 EKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQELVD 182

Query: 184 CDYKVG-GCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGYERV 243
           CD +   GC GG   SAFEFI Q GGIT E NYPY A  G C     N+  V+IDG+E V
Sbjct: 183 CDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDGHENV 242

Query: 244 PQNNEYALMKAVAHQPVAVS-------------GMLREDSFCGYRIDHTVVVVGYGSDEE 303
           P N+E AL+KAVA+QPV+V+             G+   D  C   ++H V +VGYG+  +
Sbjct: 243 PVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGD--CNTDLNHGVAIVGYGTTVD 302

Query: 304 G-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 331
           G +YWI+RN +G +WG  GY++MQR     +G+CG+AM  S+P+K
Sbjct: 303 GTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIK 345

BLAST of CSPI04G03240 vs. Swiss-Prot
Match: CEP2_ARATH (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana GN=CEP2 PE=1 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 1.7e-86
Identity = 168/345 (48.70%), Postives = 221/345 (64.06%), Query Frame = 1

Query: 3   MMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M K L++F+  +      C  F+ + K+ ESE  L  LY RW SHH + R+ +E  KRF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 63  IFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNGLHANRVG--EFM 122
           +F+ N  HV   N   +S KL+LN+FADL+ +EF   Y GSNI H+  L   + G  +FM
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFM 120

Query: 123 Y--ERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 182
           Y  E    +PSS+DWR+KGAV  IKNQG CGSCWAF+ VAAVE I++IKTN+LVSLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 183 VVDCDYKVG-GCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGY 242
           +VDCD K   GC GG    AFEFI +NGGIT E++YPY   +G C     N   VTIDG+
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 243 ERVPQNNEYALMKAVAHQPVAVSGMLREDSF-----------CGYRIDHTVVVVGYGSDE 302
           E VP+N+E AL+KAVA+QPV+V+       F           CG  ++H V  VGYGS+ 
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFTGSCGTELNHGVAAVGYGSER 300

Query: 303 EGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 331
              YWI+RN +G +WG  GY+K++R    P+G CG+AM+ S+P+K
Sbjct: 301 GKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of CSPI04G03240 vs. Swiss-Prot
Match: CYSEP_PHAVU (Vignain OS=Phaseolus vulgaris PE=2 SV=2)

HSP 1 Score: 320.1 bits (819), Expect = 2.9e-86
Identity = 168/346 (48.55%), Postives = 225/346 (65.03%), Query Frame = 1

Query: 5   KFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFKIF 64
           K L+  V+  +    +  SF+   KD  SE SL  LY+RW SHH +SR+  E HKRF +F
Sbjct: 4   KKLLWVVLSFSLVLGVANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKRFNVF 63

Query: 65  QDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITH---YNGL-HANRVGEFM 124
           + N  HV   N M K  KL+LN+FAD+++ EF   Y GS + H   + G  H N  G FM
Sbjct: 64  KANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTPHEN--GAFM 123

Query: 125 YERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV 184
           YE+ +++P S+DWR+KGAV  +K+QG CGSCWAF+ V AVE I+QIKTN+LV+LSEQE+V
Sbjct: 124 YEKVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQELV 183

Query: 185 DCDYKVG-GCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGYER 244
           DCD +   GC GG   SAFEFI Q GGIT E NYPY A  G C     N+  V+IDG+E 
Sbjct: 184 DCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGHEN 243

Query: 245 VPQNNEYALMKAVAHQPVAVS-------------GMLREDSFCGYRIDHTVVVVGYGSDE 304
           VP N+E AL+KAVA+QPV+V+             G+   D  C   ++H V +VGYG+  
Sbjct: 244 VPANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGD--CSTDLNHGVAIVGYGTTV 303

Query: 305 EG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 331
           +G +YWI+RN +G +WG +GY++MQR     +G+CG+AM PS+P+K
Sbjct: 304 DGTNYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIK 345

BLAST of CSPI04G03240 vs. Swiss-Prot
Match: CEP3_ARATH (KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana GN=CEP3 PE=2 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 3.8e-86
Identity = 164/342 (47.95%), Postives = 223/342 (65.20%), Query Frame = 1

Query: 8   IVFVVLIAFTSHLCES--FELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFKIFQ 67
           + F+VLI+F S L  S  F+ + K+ E+E ++ +LY+RW  HH +SR +HE  KRF +F+
Sbjct: 3   LFFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFR 62

Query: 68  DNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNGLHANRVGE--FMYER 127
            N  HV R N   K  KL++N+FAD++  EF   Y GSN+ H+  L   + G   FMYE 
Sbjct: 63  HNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGSGGFMYEN 122

Query: 128 AMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVDCD 187
              +PSS+DWR+KGAV  +KNQ  CGSCWAF+ VAAVE I++I+TN+LVSLSEQE+VDCD
Sbjct: 123 VTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDCD 182

Query: 188 YKVG-GCRGGNYNSAFEFIMQNGGITIEENYPYFAGN-GYCRRRGPNNERVTIDGYERVP 247
            +   GC GG    AFEFI  NGGI  EE YPY + +  +CR      E VTIDG+E VP
Sbjct: 183 TEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHVP 242

Query: 248 QNNEYALMKAVAHQPVAVSGMLREDSF-----------CGYRIDHTVVVVGYGSDEEG-D 307
           +N+E  L+KAVAHQPV+V+       F           CG +++H VV+VGYG  + G  
Sbjct: 243 ENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGECGTQLNHGVVIVGYGETKNGTK 302

Query: 308 YWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 331
           YWI+RN +G +WG  GY++++RG    +G CG+AM+ S+P K
Sbjct: 303 YWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of CSPI04G03240 vs. TrEMBL
Match: A0A0A0LY73_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G528520 PE=3 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 2.8e-157
Identity = 274/332 (82.53%), Postives = 295/332 (88.86%), Query Frame = 1

Query: 3   MMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           MMKFLIVF+VLIAF SH+CESFELE KDFESE+SLMQLYKRWSSHHRISRN HEM +RFK
Sbjct: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60

Query: 63  IFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHAN---RVGEFM 122
           +F+DNAKHVF+VNHMGKSLKL+LNQFAD+SDDEFS  YGSNIT+Y  LHA    RVG FM
Sbjct: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFM 120

Query: 123 YERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV 182
           YERA NIPSSIDWR+KGA            CWAFAAVAAVESIHQI+TNELVSLSEQEVV
Sbjct: 121 YERATNIPSSIDWRKKGARRMC--------CWAFAAVAAVESIHQIRTNELVSLSEQEVV 180

Query: 183 DCDYKVGGCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGYERV 242
           DCDYKVGGCRGG+Y SAFEFIM+NGGIT+E NYPY+AG+GYCRRRGPNNERVTIDGYE V
Sbjct: 181 DCDYKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYENV 240

Query: 243 PQNNEYALMKAVAHQPVAVSGMLREDSFCGYRIDHTVVVVGYGSDEEGDYWIIRNQYGTQ 302
           P+NNEYALMKAVAHQP    GM  E++FCG RIDHTVVVVGYGSDEEGDYWIIRNQYGTQ
Sbjct: 241 PRNNEYALMKAVAHQP----GMFTEENFCGIRIDHTVVVVGYGSDEEGDYWIIRNQYGTQ 300

Query: 303 WGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 332
           WGMNGYMKMQRGTRNPQGVCGMAM P+FPVKY
Sbjct: 301 WGMNGYMKMQRGTRNPQGVCGMAMYPAFPVKY 320

BLAST of CSPI04G03240 vs. TrEMBL
Match: A0A0A0KGB1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G490180 PE=3 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 1.9e-153
Identity = 265/351 (75.50%), Postives = 300/351 (85.47%), Query Frame = 1

Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           MT+MKFLIV +VL+AF+ ++CESFELE KDFESE+SLMQLYKRWSSHHRISRNA+EMH R
Sbjct: 1   MTVMKFLIVPLVLVAFSCNICESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHNR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHAN------- 120
           FK+F++NAKHVF+VN MGKSLKL+LNQFAD+SDDEF  MY SNIT+Y  LHA        
Sbjct: 61  FKVFKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMYSSNITYYKDLHAKKIEATGG 120

Query: 121 RVGEFMYERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSL 180
           R+G FMYE A NIPSSIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNELVSL
Sbjct: 121 RIGGFMYEHANNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSL 180

Query: 181 SEQEVVDCDYKVGGCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTI 240
           SE+EV+DCDY+ GGCRGG YNSAFEF+M N G+TIE+NYPY+ GNGYCRRRG  N+RV I
Sbjct: 181 SEEEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGRNKRVRI 240

Query: 241 DGYERVPQNNEYALMKAVAHQPVAVS-------------GMLREDSFCGYRIDHTVVVVG 300
           DGYE VP+NNEYALMKAVAHQPVAV+             GM  E+ FCG+ IDHTVVVVG
Sbjct: 241 DGYENVPRNNEYALMKAVAHQPVAVAIASGGSDFKFYGGGMFTENDFCGFNIDHTVVVVG 300

Query: 301 YGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 332
           YG+DE+GDYWIIRNQYG +WGMNGYMKMQRG  +PQGVCGMAMQP++PVKY
Sbjct: 301 YGTDEDGDYWIIRNQYGHRWGMNGYMKMQRGAHSPQGVCGMAMQPAYPVKY 351

BLAST of CSPI04G03240 vs. TrEMBL
Match: A0A0A0KW34_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G013580 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.1e-124
Identity = 221/225 (98.22%), Postives = 223/225 (99.11%), Query Frame = 1

Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           MTMMKFLIV VVLIAFT+HLCESFELE KDFESE+SLMQLYKRWSSHHRISRNAHEMHKR
Sbjct: 1   MTMMKFLIVLVVLIAFTTHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHANRVGEFMY 120
           FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHANRVGEFMY
Sbjct: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHANRVGEFMY 120

Query: 121 ERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVD 180
           ERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVD
Sbjct: 121 ERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVD 180

Query: 181 CDYKVGGCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRG 226
           CDYKVGGCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRG
Sbjct: 181 CDYKVGGCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRG 225

BLAST of CSPI04G03240 vs. TrEMBL
Match: A0A0A0KXE7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G015180 PE=3 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 4.3e-105
Identity = 191/193 (98.96%), Postives = 192/193 (99.48%), Query Frame = 1

Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR
Sbjct: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHANRVGEFMY 120
           FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHANRVGEFMY
Sbjct: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHANRVGEFMY 120

Query: 121 ERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVD 180
           ERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQI+TNELVSLSEQ VVD
Sbjct: 121 ERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIRTNELVSLSEQAVVD 180

Query: 181 CDYKVGGCRGGNY 194
           CDYKVGGCRGGNY
Sbjct: 181 CDYKVGGCRGGNY 193

BLAST of CSPI04G03240 vs. TrEMBL
Match: A0A0A0LMU4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G349680 PE=3 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 7.4e-97
Identity = 180/346 (52.02%), Postives = 242/346 (69.94%), Query Frame = 1

Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           M + KFL+V ++LI   S L ESFE + K+  +E SL QLY+RW  HH ISRN  E HKR
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGKHHTISRNLKEKHKR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYG-SNITHYNGLHANR--VGE 120
           F +F++N  HVF VN M K  KL+LN+FAD+S+ EF   Y  SNI+HY  LH  R   G 
Sbjct: 61  FSVFKENVNHVFTVNQMDKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLHERRRGAGG 120

Query: 121 FMYERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 180
           FMYE+  ++PSS+DWR++GAVNA+K QG CGSCWAF++VAAVE I++IKTN+L+SLSEQE
Sbjct: 121 FMYEQDTDLPSSVDWRERGAVNAVKEQGRCGSCWAFSSVAAVEGINKIKTNQLLSLSEQE 180

Query: 181 VVDCDYKVGGCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGYE 240
           ++DC+Y+  GC GG    AF+FI +NGGI  E +YPY    G CR    ++  V IDGYE
Sbjct: 181 LLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGYE 240

Query: 241 RVPQNNEYALMKAVAHQP--VAVSGMLRE---------DSFCGYRIDHTVVVVGYGSDEE 300
            VP+ NE ALM+AVA+QP  VA+    R+         D +CG  ++H VV +GYG+ E+
Sbjct: 241 SVPE-NEDALMQAVANQPVSVAIDAAGRDFQFYSQGVFDGYCGTELNHGVVAIGYGTTED 300

Query: 301 G-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 332
           G DYW++RN +G  WG +GY++M+RG    +G+CG+AM+ S+P+KY
Sbjct: 301 GTDYWLVRNSWGVGWGEDGYVRMKRGVEQAEGLCGIAMEASYPIKY 345

BLAST of CSPI04G03240 vs. TAIR10
Match: AT3G48340.1 (AT3G48340.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 320.9 bits (821), Expect = 9.5e-88
Identity = 168/345 (48.70%), Postives = 221/345 (64.06%), Query Frame = 1

Query: 3   MMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M K L++F+  +      C  F+ + K+ ESE  L  LY RW SHH + R+ +E  KRF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 63  IFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNGLHANRVG--EFM 122
           +F+ N  HV   N   +S KL+LN+FADL+ +EF   Y GSNI H+  L   + G  +FM
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFM 120

Query: 123 Y--ERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 182
           Y  E    +PSS+DWR+KGAV  IKNQG CGSCWAF+ VAAVE I++IKTN+LVSLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 183 VVDCDYKVG-GCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGY 242
           +VDCD K   GC GG    AFEFI +NGGIT E++YPY   +G C     N   VTIDG+
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 243 ERVPQNNEYALMKAVAHQPVAVSGMLREDSF-----------CGYRIDHTVVVVGYGSDE 302
           E VP+N+E AL+KAVA+QPV+V+       F           CG  ++H V  VGYGS+ 
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFTGSCGTELNHGVAAVGYGSER 300

Query: 303 EGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 331
              YWI+RN +G +WG  GY+K++R    P+G CG+AM+ S+P+K
Sbjct: 301 GKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of CSPI04G03240 vs. TAIR10
Match: AT3G48350.1 (AT3G48350.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 319.7 bits (818), Expect = 2.1e-87
Identity = 164/342 (47.95%), Postives = 223/342 (65.20%), Query Frame = 1

Query: 8   IVFVVLIAFTSHLCES--FELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFKIFQ 67
           + F+VLI+F S L  S  F+ + K+ E+E ++ +LY+RW  HH +SR +HE  KRF +F+
Sbjct: 3   LFFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFR 62

Query: 68  DNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNGLHANRVGE--FMYER 127
            N  HV R N   K  KL++N+FAD++  EF   Y GSN+ H+  L   + G   FMYE 
Sbjct: 63  HNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGSGGFMYEN 122

Query: 128 AMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVDCD 187
              +PSS+DWR+KGAV  +KNQ  CGSCWAF+ VAAVE I++I+TN+LVSLSEQE+VDCD
Sbjct: 123 VTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDCD 182

Query: 188 YKVG-GCRGGNYNSAFEFIMQNGGITIEENYPYFAGN-GYCRRRGPNNERVTIDGYERVP 247
            +   GC GG    AFEFI  NGGI  EE YPY + +  +CR      E VTIDG+E VP
Sbjct: 183 TEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHVP 242

Query: 248 QNNEYALMKAVAHQPVAVSGMLREDSF-----------CGYRIDHTVVVVGYGSDEEG-D 307
           +N+E  L+KAVAHQPV+V+       F           CG +++H VV+VGYG  + G  
Sbjct: 243 ENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGECGTQLNHGVVIVGYGETKNGTK 302

Query: 308 YWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 331
           YWI+RN +G +WG  GY++++RG    +G CG+AM+ S+P K
Sbjct: 303 YWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of CSPI04G03240 vs. TAIR10
Match: AT5G50260.1 (AT5G50260.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 303.5 bits (776), Expect = 1.6e-82
Identity = 156/343 (45.48%), Postives = 213/343 (62.10%), Query Frame = 1

Query: 4   MKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFKI 63
           MK  IV  + +       +  +   KD ESE SL +LY+RW SHH ++R+  E  KRF +
Sbjct: 1   MKRFIVLALCMLMVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNV 60

Query: 64  FQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNGLHANRVG--EFMY 123
           F+ N KH+   N   KS KL+LN+F D++ +EF   Y GSNI H+      +     FMY
Sbjct: 61  FKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKKATKSFMY 120

Query: 124 ERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVD 183
                +P+S+DWR+ GAV  +KNQG CGSCWAF+ V AVE I+QI+T +L SLSEQE+VD
Sbjct: 121 ANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSEQELVD 180

Query: 184 CDYKVG-GCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGYERV 243
           CD     GC GG  + AFEFI + GG+T E  YPY A +  C     N   V+IDG+E V
Sbjct: 181 CDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSIDGHEDV 240

Query: 244 PQNNEYALMKAVAHQPVAVSGMLREDSF-----------CGYRIDHTVVVVGYGSDEEG- 303
           P+N+E  LMKAVA+QPV+V+       F           CG  ++H V VVGYG+  +G 
Sbjct: 241 PKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVFTGRCGTELNHGVAVVGYGTTIDGT 300

Query: 304 DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 331
            YWI++N +G +WG  GY++MQRG R+ +G+CG+AM+ S+P+K
Sbjct: 301 KYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLK 343

BLAST of CSPI04G03240 vs. TAIR10
Match: AT1G20850.1 (AT1G20850.1 xylem cysteine peptidase 2)

HSP 1 Score: 271.2 bits (692), Expect = 8.7e-73
Identity = 140/316 (44.30%), Postives = 192/316 (60.76%), Query Frame = 1

Query: 29  KDFESERSLMQLYKRW-SSHHRISRNAHEMHKRFKIFQDNAKHVFRVNHMGKSLKLRLNQ 88
           +D ES   L++L++ W S+  +      E   RF++F+DN KH+   N  GKS  L LN+
Sbjct: 39  EDLESHDKLIELFENWISNFEKAYETVEEKFLRFEVFKDNLKHIDETNKKGKSYWLGLNE 98

Query: 89  FADLSDDEFSMMY-GSNITHYNGLHANRVGEFMYERAMNIPSSIDWRQKGAVNAIKNQGH 148
           FADLS +EF  MY G               EF Y     +P S+DWR+KGAV  +KNQG 
Sbjct: 99  FADLSHEEFKKMYLGLKTDIVRRDEERSYAEFAYRDVEAVPKSVDWRKKGAVAEVKNQGS 158

Query: 149 CGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVDCDYKV-GGCRGGNYNSAFEFIMQNGG 208
           CGSCWAF+ VAAVE I++I T  L +LSEQE++DCD     GC GG  + AFE+I++NGG
Sbjct: 159 CGSCWAFSTVAAVEGINKIVTGNLTTLSEQELIDCDTTYNNGCNGGLMDYAFEYIVKNGG 218

Query: 209 ITIEENYPYFAGNGYCRRRGPNNERVTIDGYERVPQNNEYALMKAVAHQP--VAVSGMLR 268
           +  EE+YPY    G C  +   +E VTI+G++ VP N+E +L+KA+AHQP  VA+    R
Sbjct: 219 LRKEEDYPYSMEEGTCEMQKDESETVTINGHQDVPTNDEKSLLKALAHQPLSVAIDASGR 278

Query: 269 E---------DSFCGYRIDHTVVVVGYGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRN 328
           E         D  CG  +DH V  VGYGS +  DY I++N +G +WG  GY++++R T  
Sbjct: 279 EFQFYSGGVFDGRCGVDLDHGVAAVGYGSSKGSDYIIVKNSWGPKWGEKGYIRLKRNTGK 338

Query: 329 PQGVCGMAMQPSFPVK 331
           P+G+CG+    SFP K
Sbjct: 339 PEGLCGINKMASFPTK 354

BLAST of CSPI04G03240 vs. TAIR10
Match: AT3G19400.1 (AT3G19400.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 267.3 bits (682), Expect = 1.3e-71
Identity = 153/340 (45.00%), Postives = 213/340 (62.65%), Query Frame = 1

Query: 8   IVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNA-HEMHKRFKIFQD 67
           +V + ++  +S L  + E E +  E+E  LM  Y++W   +R + N   E  +RFKIF+D
Sbjct: 13  LVILSVLLLSSSLGVATETEIERNETEVRLM--YEQWLVENRKNYNGLGEKERRFKIFKD 72

Query: 68  NAKHVFRVNHM-GKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHANRVGEFMYERAMN 127
           N K V   N +  ++ ++ L +FADL+++EF  +Y           + +   ++Y+    
Sbjct: 73  NLKFVDEHNSVPDRTFEVGLTRFADLTNEEFRAIYLRKKMERTK-DSVKTERYLYKEGDV 132

Query: 128 IPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVDCD--Y 187
           +P  +DWR  GAV ++K+QG+CGSCWAF+AV AVE I+QI T EL+SLSEQE+VDCD  +
Sbjct: 133 LPDEVDWRANGAVVSVKDQGNCGSCWAFSAVGAVEGINQITTGELISLSEQELVDCDRGF 192

Query: 188 KVGGCRGGNYNSAFEFIMQNGGITIEENYPYFAGN-GYCRRRGPNNER-VTIDGYERVPQ 247
              GC GG  N AFEFIM+NGGI  +++YPY A + G C     NN R VTIDGYE VP+
Sbjct: 193 VNAGCDGGIMNYAFEFIMKNGGIETDQDYPYNANDLGLCNADKNNNTRVVTIDGYEDVPR 252

Query: 248 NNEYALMKAVAHQPVAVSGMLREDSF-----------CGYRIDHTVVVVGYGSDEEGDYW 307
           ++E +L KAVAHQPV+V+      +F           CG  +DH VVVVGYGS    DYW
Sbjct: 253 DDEKSLKKAVAHQPVSVAIEASSQAFQLYKSGVMTGTCGISLDHGVVVVGYGSTSGEDYW 312

Query: 308 IIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 331
           IIRN +G  WG +GY+K+QR   +P G CG+AM PS+P K
Sbjct: 313 IIRNSWGLNWGDSGYVKLQRNIDDPFGKCGIAMMPSYPTK 349

BLAST of CSPI04G03240 vs. NCBI nr
Match: gi|778697454|ref|XP_011654324.1| (PREDICTED: vignain-like [Cucumis sativus])

HSP 1 Score: 571.6 bits (1472), Expect = 8.8e-160
Identity = 285/331 (86.10%), Postives = 288/331 (87.01%), Query Frame = 1

Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           MTMMKFLIV VVLIAFT+HLCESFELE KDFESE+SLMQLYKRWSSHHRISRNAHEMHKR
Sbjct: 1   MTMMKFLIVLVVLIAFTTHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHANRVGEFMY 120
           FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHANRVGEFMY
Sbjct: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHANRVGEFMY 120

Query: 121 ERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVD 180
           ERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVD
Sbjct: 121 ERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVD 180

Query: 181 CDYKVGGCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGYERVP 240
           CDYKVGGCRGGNYNSAFEFIMQNGGITIEENYPYFA                        
Sbjct: 181 CDYKVGGCRGGNYNSAFEFIMQNGGITIEENYPYFA------------------------ 240

Query: 241 QNNEYALMKAVAHQPVAVSGMLREDSFCGYRIDHTVVVVGYGSDEEGDYWIIRNQYGTQW 300
             N Y   +          GMLRE SFCGYRIDHTVVVVGYGSDEEGDYWIIRNQYGTQW
Sbjct: 241 -GNGYCRRRG---------GMLREGSFCGYRIDHTVVVVGYGSDEEGDYWIIRNQYGTQW 297

Query: 301 GMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 332
           GMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY
Sbjct: 301 GMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 297

BLAST of CSPI04G03240 vs. NCBI nr
Match: gi|659108975|ref|XP_008454483.1| (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 568.2 bits (1463), Expect = 9.7e-159
Identity = 277/346 (80.06%), Postives = 304/346 (87.86%), Query Frame = 1

Query: 2   TMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRF 61
           T+MKFLIV +VLIA TSHLCESFELE KDFESE+SLMQLYKRWSSHHRISRNA+EMHKRF
Sbjct: 3   TVMKFLIVPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 62  KIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHAN--RVGEFM 121
           K+F+DNAKHVF+ NHMG+SLKL+LNQFAD+SDDEFS ++GSNIT+Y  LHA    VG FM
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKTGHVGGFM 122

Query: 122 YERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV 181
           YE A  IPSSIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV
Sbjct: 123 YEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV 182

Query: 182 DCDYKVGGCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGYERV 241
           DCDY+ GGCRGG+YNSAFEF+M+NGGIT+E+NYPY+ G+GYCRRRG  NERV IDGYE V
Sbjct: 183 DCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGYENV 242

Query: 242 PQNNEYALMKAVAHQPVAVS-------------GMLREDSFCGYRIDHTVVVVGYGSDEE 301
           P+NNE+ALMKAVAHQPVAV+             GM  E  FCGY IDHTVVVVGYGSDEE
Sbjct: 243 PRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYGSDEE 302

Query: 302 -GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 332
            GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMAMQP++PVKY
Sbjct: 303 DGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 348

BLAST of CSPI04G03240 vs. NCBI nr
Match: gi|700210691|gb|KGN65787.1| (hypothetical protein Csa_1G528520 [Cucumis sativus])

HSP 1 Score: 562.8 bits (1449), Expect = 4.1e-157
Identity = 274/332 (82.53%), Postives = 295/332 (88.86%), Query Frame = 1

Query: 3   MMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           MMKFLIVF+VLIAF SH+CESFELE KDFESE+SLMQLYKRWSSHHRISRN HEM +RFK
Sbjct: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60

Query: 63  IFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHAN---RVGEFM 122
           +F+DNAKHVF+VNHMGKSLKL+LNQFAD+SDDEFS  YGSNIT+Y  LHA    RVG FM
Sbjct: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFM 120

Query: 123 YERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV 182
           YERA NIPSSIDWR+KGA            CWAFAAVAAVESIHQI+TNELVSLSEQEVV
Sbjct: 121 YERATNIPSSIDWRKKGARRMC--------CWAFAAVAAVESIHQIRTNELVSLSEQEVV 180

Query: 183 DCDYKVGGCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGYERV 242
           DCDYKVGGCRGG+Y SAFEFIM+NGGIT+E NYPY+AG+GYCRRRGPNNERVTIDGYE V
Sbjct: 181 DCDYKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYENV 240

Query: 243 PQNNEYALMKAVAHQPVAVSGMLREDSFCGYRIDHTVVVVGYGSDEEGDYWIIRNQYGTQ 302
           P+NNEYALMKAVAHQP    GM  E++FCG RIDHTVVVVGYGSDEEGDYWIIRNQYGTQ
Sbjct: 241 PRNNEYALMKAVAHQP----GMFTEENFCGIRIDHTVVVVGYGSDEEGDYWIIRNQYGTQ 300

Query: 303 WGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 332
           WGMNGYMKMQRGTRNPQGVCGMAM P+FPVKY
Sbjct: 301 WGMNGYMKMQRGTRNPQGVCGMAMYPAFPVKY 320

BLAST of CSPI04G03240 vs. NCBI nr
Match: gi|659101492|ref|XP_008451632.1| (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 560.8 bits (1444), Expect = 1.6e-156
Identity = 271/347 (78.10%), Postives = 300/347 (86.46%), Query Frame = 1

Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           M +MKF IV ++LIAF SHLCESFELE KDFESE++LMQLYKRWSSHHRISRNA+EMHKR
Sbjct: 1   MAVMKFFIVPLILIAFMSHLCESFELERKDFESEKNLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHAN---RVGE 120
           FK+F+DNAK VF+ N MGKSLKL+LNQFAD+SDDEF  ++ SNITHY  LHA    RVG 
Sbjct: 61  FKVFKDNAKQVFKKNQMGKSLKLKLNQFADMSDDEFRSIHSSNITHYKNLHAKTIGRVGG 120

Query: 121 FMYERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 180
           FMYE A  IPSSIDWR+KGAVNAIK+QG CGSCWAFAAVAAVESIHQIKTNELVSLSEQE
Sbjct: 121 FMYEHAKEIPSSIDWRKKGAVNAIKDQGRCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 180

Query: 181 VVDCDYKVGGCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGYE 240
           VVDCDYK GGCRGG+YNSAFEFIM+N G+T E+NYPYF G+GYCRRRG   ERVTIDGYE
Sbjct: 181 VVDCDYKDGGCRGGHYNSAFEFIMENDGVTAEDNYPYFEGDGYCRRRGGYKERVTIDGYE 240

Query: 241 RVPQNNEYALMKAVAHQPVAVS-------------GMLREDSFCGYRIDHTVVVVGYGSD 300
            VP+NNE+ALMKAVAHQPVAV+             GM  E++FCGY IDHTVVVVGYG+D
Sbjct: 241 NVPRNNEHALMKAVAHQPVAVAIASGGFDFKFYGQGMFTEENFCGYNIDHTVVVVGYGTD 300

Query: 301 EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 332
           E+GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMAMQP++PVKY
Sbjct: 301 EDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 347

BLAST of CSPI04G03240 vs. NCBI nr
Match: gi|659108973|ref|XP_008454482.1| (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 559.3 bits (1440), Expect = 4.5e-156
Identity = 273/347 (78.67%), Postives = 304/347 (87.61%), Query Frame = 1

Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           M +MKFLIV +VLIAFT HLCESFELE KDFESE+SLMQLYKRWSSHHRISRNA+EMHKR
Sbjct: 1   MAVMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHAN--RVGEF 120
           FK+F+DNAK+VF+ NHMG+SLKL+LNQFAD+SDDEFS ++GSNIT+Y  LHA   RVG F
Sbjct: 61  FKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKNGRVGGF 120

Query: 121 MYERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEV 180
           MYE A +IPSSIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNELVSLSEQEV
Sbjct: 121 MYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSEQEV 180

Query: 181 VDCDYKVGGCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGYER 240
           VDCDY+  GC GG YNSAFEF+M+NGGIT+E+NYPY+ G+GYCRRRG  NERVTIDGYE 
Sbjct: 181 VDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDGYEN 240

Query: 241 VPQNNEYALMKAVAHQPVAVS-------------GMLREDSFCGYRIDHTVVVVGYGSDE 300
           VP+NNE+ALMKAVAHQPVAV+             GM  E  FCGY IDHTVVVVGYG+DE
Sbjct: 241 VPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYGTDE 300

Query: 301 E-GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 332
           E GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMA+QP++PVK+
Sbjct: 301 EDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAIQPAYPVKH 347

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CYSEP_RICCO1.5e-8748.55Vignain OS=Ricinus communis GN=CYSEP PE=1 SV=1[more]
CYSEP_VIGMU1.5e-8748.12Vignain OS=Vigna mungo PE=1 SV=1[more]
CEP2_ARATH1.7e-8648.70KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana GN=CEP2 PE=1 SV=... [more]
CYSEP_PHAVU2.9e-8648.55Vignain OS=Phaseolus vulgaris PE=2 SV=2[more]
CEP3_ARATH3.8e-8647.95KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana GN=CEP3 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LY73_CUCSA2.8e-15782.53Uncharacterized protein OS=Cucumis sativus GN=Csa_1G528520 PE=3 SV=1[more]
A0A0A0KGB1_CUCSA1.9e-15375.50Uncharacterized protein OS=Cucumis sativus GN=Csa_6G490180 PE=3 SV=1[more]
A0A0A0KW34_CUCSA1.1e-12498.22Uncharacterized protein OS=Cucumis sativus GN=Csa_4G013580 PE=3 SV=1[more]
A0A0A0KXE7_CUCSA4.3e-10598.96Uncharacterized protein OS=Cucumis sativus GN=Csa_4G015180 PE=3 SV=1[more]
A0A0A0LMU4_CUCSA7.4e-9752.02Uncharacterized protein OS=Cucumis sativus GN=Csa_2G349680 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G48340.19.5e-8848.70 Cysteine proteinases superfamily protein[more]
AT3G48350.12.1e-8747.95 Cysteine proteinases superfamily protein[more]
AT5G50260.11.6e-8245.48 Cysteine proteinases superfamily protein[more]
AT1G20850.18.7e-7344.30 xylem cysteine peptidase 2[more]
AT3G19400.11.3e-7145.00 Cysteine proteinases superfamily protein[more]
Match NameE-valueIdentityDescription
gi|778697454|ref|XP_011654324.1|8.8e-16086.10PREDICTED: vignain-like [Cucumis sativus][more]
gi|659108975|ref|XP_008454483.1|9.7e-15980.06PREDICTED: ervatamin-B-like [Cucumis melo][more]
gi|700210691|gb|KGN65787.1|4.1e-15782.53hypothetical protein Csa_1G528520 [Cucumis sativus][more]
gi|659101492|ref|XP_008451632.1|1.6e-15678.10PREDICTED: ervatamin-B-like [Cucumis melo][more]
gi|659108973|ref|XP_008454482.1|4.5e-15678.67PREDICTED: ervatamin-B-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000169Pept_cys_AS
IPR000668Peptidase_C1A_C
IPR013128Peptidase_C1A
IPR013201Prot_inhib_I29
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0008234cysteine-type peptidase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0008234 cysteine-type peptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G03240.1CSPI04G03240.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 144..155
scor
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 144..159
score: 1.5E-9coord: 274..284
score: 1.5E-9coord: 289..295
score: 1.
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 126..328
score: 1.1
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 126..329
score: 1.6
IPR013128Peptidase C1APANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 3..330
score: 3.4E
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 41..96
score: 8.4
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 41..96
score: 1.
NoneNo IPR availableGENE3DG3DSA:3.90.70.10coord: 7..329
score: 2.1
NoneNo IPR availablePANTHERPTHR12411:SF346KDEL-TAILED CYSTEINE ENDOPEPTIDASE CEP1-RELATEDcoord: 3..330
score: 3.4E
NoneNo IPR availableunknownSSF54001Cysteine proteinasescoord: 37..329
score: 4.74