CSPI02G14340 (gene) Wild cucumber (PI 183967)

NameCSPI02G14340
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionEF hand calcium-binding family protein
LocationChr2 : 14164740 .. 14166059 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTAAACTATAGAATAAAAAAGTATTATTTTTAAAAAATATAGGAAAAATAAATTTATATAGGAGGGATATAAATAGAGGGTTGATGGTTATACATTAATTGTGATTGGCATATAGGAAGAGATGTCAAGCTTATTGAAGTTATTCAGAAGCTGTTCTGGAAAGCGACCATCCAAAAACACTTCCTTTTCCATACCAATCAAAGAAAGGTCCAATTCTTCCTCACCCTCTTCAAAGCAATTCCGCCCAAACTTGGAGGAGATCAAATGGGTATGAACAATTCTTTTTTTGCATGAATTATTTCAAATCTTTTTATGAAAAAAACATTGAATTATTTCAAATGTTCATAAGCAATAAAGTTGCTGAGAAAGTTATGAACTGGAACTCTCTATAACAACATGCTGTGCTACGTACGTTCGTAGTAATGATCTCTTTTGGATTTAATAGGCGTTTGAGAAGTTTGACTCGAACAAGGATGGAAAAATCTCATTTGAGGAGTACAAGGAAGCTCACCGAGGCTTGGCTGGAAGCAAAGAGATCACAGACGCAGAGGCAGAGAAGTCATTCAAGCTTGTGGATGTAGATGGCGATGGATTCGTGGATTTGAAGGAGTTCGTGGAGCTGTACACGATGAGCAGCGGGGAAGTGAAGGTGGGCGACATCGAAAGTGCCTTCAAAGTTTATGATTCCAATGGAGATGGGAAGATAAGTGCAGAGGAGGTGATGGGGATCATGAAGATATTGGGGGAGAACACCACCTTGAAGGCTTGCAAGCAAATGGTGAAGGGTGTGGACATGGATGGGGATGGCTTTATTGATGTCCAAGAGTTTTCTAAGCTCATGGGTAAGTATTCGAGTAAGTTTCAAAATTAAAGAACCTAAAACTACAACTTCGGACGCCCCGTATCCATTTTAATTTTTCTTGAGTGTAACGGTTGATCTCTAGCTTAATTTTCAAAAACAAAAACAAAAAAATAAAATGATTGGAAAACGAGAGCTACACATGTCTCTAGTTTTTAGACAAATGAATGTGGAAAACAAAAATTCTAAGCTATATTTGAAGTTTGTATCCAATAATTAGGAGGATAGTTATATTGTGACAATGTTGGTGATTAATGTGCGACTACTAGTAGTTATTATGCAACGTGTAGATTTGTGATGTAATTTTTGTTAAGTTGTCAACATCTTCAAACGATGAAGGGTTCAATATTGATATAGTGAATTGAAAATCTCTATTATATAAGAAAATTGATGTCAAGATTACTTTTATTGATATTTTCGTCAAGATTTACATTATTGTCAACATTCTTATGATCGGTG

mRNA sequence

ATGTCAAGCTTATTGAAGTTATTCAGAAGCTGTTCTGGAAAGCGACCATCCAAAAACACTTCCTTTTCCATACCAATCAAAGAAAGGTCCAATTCTTCCTCACCCTCTTCAAAGCAATTCCGCCCAAACTTGGAGGAGATCAAATGGGCGTTTGAGAAGTTTGACTCGAACAAGGATGGAAAAATCTCATTTGAGGAGTACAAGGAAGCTCACCGAGGCTTGGCTGGAAGCAAAGAGATCACAGACGCAGAGGCAGAGAAGTCATTCAAGCTTGTGGATGTAGATGGCGATGGATTCGTGGATTTGAAGGAGTTCGTGGAGCTGTACACGATGAGCAGCGGGGAAGTGAAGGTGGGCGACATCGAAAGTGCCTTCAAAGTTTATGATTCCAATGGAGATGGGAAGATAAGTGCAGAGGAGGTGATGGGGATCATGAAGATATTGGGGGAGAACACCACCTTGAAGGCTTGCAAGCAAATGGTGAAGGGTGTGGACATGGATGGGGATGGCTTTATTGATGTCCAAGAGTTTTCTAAGCTCATGGGTAAGTATTCGAGTAAGTTTCAAAATTAA

Coding sequence (CDS)

ATGTCAAGCTTATTGAAGTTATTCAGAAGCTGTTCTGGAAAGCGACCATCCAAAAACACTTCCTTTTCCATACCAATCAAAGAAAGGTCCAATTCTTCCTCACCCTCTTCAAAGCAATTCCGCCCAAACTTGGAGGAGATCAAATGGGCGTTTGAGAAGTTTGACTCGAACAAGGATGGAAAAATCTCATTTGAGGAGTACAAGGAAGCTCACCGAGGCTTGGCTGGAAGCAAAGAGATCACAGACGCAGAGGCAGAGAAGTCATTCAAGCTTGTGGATGTAGATGGCGATGGATTCGTGGATTTGAAGGAGTTCGTGGAGCTGTACACGATGAGCAGCGGGGAAGTGAAGGTGGGCGACATCGAAAGTGCCTTCAAAGTTTATGATTCCAATGGAGATGGGAAGATAAGTGCAGAGGAGGTGATGGGGATCATGAAGATATTGGGGGAGAACACCACCTTGAAGGCTTGCAAGCAAATGGTGAAGGGTGTGGACATGGATGGGGATGGCTTTATTGATGTCCAAGAGTTTTCTAAGCTCATGGGTAAGTATTCGAGTAAGTTTCAAAATTAA
BLAST of CSPI02G14340 vs. Swiss-Prot
Match: CML1_ARATH (Calmodulin-like protein 1 OS=Arabidopsis thaliana GN=CML1 PE=2 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.1e-29
Identity = 77/177 (43.50%), Postives = 113/177 (63.84%), Query Frame = 1

Query: 6   KLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKQF-RPNLEEIKWAFEKFDSNKDGKISF 65
           KL ++   ++PS+  S     ++ S  SSP    F +P++ E++  F +FD +KDGKIS 
Sbjct: 12  KLSKNKMLRKPSRMFSRD---RQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLDKDGKISQ 71

Query: 66  EEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGDIESA 125
            EYK   R L   + I D    K FK VD+DGDGF+D +EF++ Y  S G ++  DI ++
Sbjct: 72  TEYKVVLRALGQERAIEDVP--KIFKAVDLDGDGFIDFREFIDAYKRSGG-IRSSDIRNS 131

Query: 126 FKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKLM 182
           F  +D NGDGKISAEEVM ++  LGE  +L+ C +MV+ VD DGDG ++++EF K+M
Sbjct: 132 FWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKMM 182

BLAST of CSPI02G14340 vs. Swiss-Prot
Match: CML24_ARATH (Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 8.1e-25
Identity = 61/144 (42.36%), Postives = 93/144 (64.58%), Query Frame = 1

Query: 43  NLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDL 102
           ++++IK  F++FD N DGKIS +E KE  R L  S   +  E     K  D+DG+GF+DL
Sbjct: 14  SMDDIKKVFQRFDKNGDGKISVDELKEVIRAL--SPTASPEETVTMMKQFDLDGNGFIDL 73

Query: 103 KEFVELYTMSSG-----EVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKAC 162
            EFV L+ +  G        V D++ AF++YD +G+G+ISA+E+  +MK LGE  +++ C
Sbjct: 74  DEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDC 133

Query: 163 KQMVKGVDMDGDGFIDVQEFSKLM 182
           K+M+  VD+DGDG ++  EF K+M
Sbjct: 134 KKMISKVDIDGDGCVNFDEFKKMM 155

BLAST of CSPI02G14340 vs. Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.1e-24
Identity = 59/142 (41.55%), Postives = 91/142 (64.08%), Query Frame = 1

Query: 43  NLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDL 102
           ++E+IK  F++FD N DGKIS +E K+    L  S   +  E +   K  D+DG+GF+DL
Sbjct: 12  SMEDIKKVFQRFDKNNDGKISIDELKDVIGAL--SPNASQEETKAMMKEFDLDGNGFIDL 71

Query: 103 KEFVELYTMS---SGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQ 162
            EFV L+ +S   S    + D++ AF +YD + +G+ISA E+  +MK LGE  +++ C++
Sbjct: 72  DEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQR 131

Query: 163 MVKGVDMDGDGFIDVQEFSKLM 182
           M+  VD DGDG +D +EF K+M
Sbjct: 132 MINKVDSDGDGCVDFEEFKKMM 151

BLAST of CSPI02G14340 vs. Swiss-Prot
Match: CML32_ARATH (Probable calcium-binding protein CML32 OS=Arabidopsis thaliana GN=CML32 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.4e-23
Identity = 63/143 (44.06%), Postives = 86/143 (60.14%), Query Frame = 1

Query: 51  FEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYT 110
           FE+ D NKDGKIS++E+ EA R  A S  IT  E +  F+ +DVDGD  +D+ E+     
Sbjct: 7   FERVDKNKDGKISWDEFAEAIR--AFSPSITSEEIDNMFREIDVDGDNQIDVAEYASCL- 66

Query: 111 MSSGEVKVGD----IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDM 170
           M  GE    D    ++ AF +YD +GDGKISA E+  ++K LGE  T+  C  MV+ VD 
Sbjct: 67  MLGGEGNKEDEDIVMKEAFDLYDIDGDGKISASEIHVVLKRLGEKQTIAECIAMVRAVDA 126

Query: 171 DGDGFIDVQEFSKLMGKYSSKFQ 190
           DGDGF+  +EF  +M   + K Q
Sbjct: 127 DGDGFVSFEEFKTMMSCNNKKLQ 146

BLAST of CSPI02G14340 vs. Swiss-Prot
Match: CML25_ARATH (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.2e-21
Identity = 56/142 (39.44%), Postives = 88/142 (61.97%), Query Frame = 1

Query: 41  RPNLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFV 100
           +  + E++  F+KFD N DGKIS +E       L    E+ + E EK+   +D  GDG++
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLG--HEVPEEELEKAITEIDRKGDGYI 91

Query: 101 DLKEFVELYTMSSGEVKV-GDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQ 160
           + +EFVEL T    +  V  +++ AF VYD +G+G ISAEE+  +++ LG+  ++  C++
Sbjct: 92  NFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRK 151

Query: 161 MVKGVDMDGDGFIDVQEFSKLM 182
           M+ GVD DGDG ID +EF  +M
Sbjct: 152 MIGGVDKDGDGTIDFEEFKIMM 171

BLAST of CSPI02G14340 vs. TrEMBL
Match: A0A0A0LMP3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G286450 PE=4 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 2.2e-98
Identity = 189/190 (99.47%), Postives = 189/190 (99.47%), Query Frame = 1

Query: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKQFRPNLEEIKWAFEKFDSNKDG 60
           MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSK FRPNLEEIKWAFEKFDSNKDG
Sbjct: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDG 60

Query: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120
           KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD
Sbjct: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120

Query: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180
           IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL
Sbjct: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180

Query: 181 MGKYSSKFQN 191
           MGKYSSKFQN
Sbjct: 181 MGKYSSKFQN 190

BLAST of CSPI02G14340 vs. TrEMBL
Match: W9RW36_9ROSA (Calmodulin-like protein 1 OS=Morus notabilis GN=L484_002706 PE=4 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 4.3e-41
Identity = 85/147 (57.82%), Postives = 113/147 (76.87%), Query Frame = 1

Query: 44  LEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLK 103
           +EE++WAFEKFD+N+DGKIS EEYK A R L   +EITD E  K+F  +D DGDGFVD K
Sbjct: 1   MEELRWAFEKFDTNRDGKISREEYKSALRVL--DREITDGEIAKAFNALDTDGDGFVDFK 60

Query: 104 EFVELYTMSSGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKG 163
           EFVE++ M SG VK  DIESAF+++D +GDGKISAEE+  ++K LGE+ +++AC+ MVKG
Sbjct: 61  EFVEMFDMGSGGVKASDIESAFRLFDLDGDGKISAEELSQVLKQLGESCSIRACRNMVKG 120

Query: 164 VDMDGDGFIDVQEFSKLMGKYSSKFQN 191
           VD DGDGFI+++EF+++M        N
Sbjct: 121 VDNDGDGFINIEEFTRMMSTTKKPITN 145

BLAST of CSPI02G14340 vs. TrEMBL
Match: I1LHF1_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_11G056500 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 4.5e-38
Identity = 87/172 (50.58%), Postives = 124/172 (72.09%), Query Frame = 1

Query: 10  SCSGKRPSKNTSFSIPIKERSNSSSPSSKQFRPNLEEIKWAFEKFDSNKDGKISFEEYKE 69
           S S K  +K  SF       S  +S   + F+P  EE+KW F+KFD+N+DGK+S EEYK 
Sbjct: 18  SSSRKNVAKKNSFV------SRQTSNEGQSFQPTKEEMKWVFQKFDTNRDGKVSLEEYKA 77

Query: 70  AHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGDIESAFKVYD 129
           A R L   + I +AEA K+F+++D+DGDGF+DL EF+E++    G +K  +I++AF+V+D
Sbjct: 78  AARAL--DRAIGEAEAVKAFRVMDIDGDGFIDLNEFMEMFN-GEGRIKETEIKNAFQVFD 137

Query: 130 SNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKLM 182
            NGDGKISAEE+  ++K LGE+ +L ACK+MVKGVD +GDGFID+ EF+++M
Sbjct: 138 LNGDGKISAEELSHVLKRLGESCSLSACKKMVKGVDGNGDGFIDLNEFTRMM 180

BLAST of CSPI02G14340 vs. TrEMBL
Match: A0A0B2SA84_GLYSO (Calmodulin-like protein 1 OS=Glycine soja GN=glysoja_003815 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 4.5e-38
Identity = 87/172 (50.58%), Postives = 124/172 (72.09%), Query Frame = 1

Query: 10  SCSGKRPSKNTSFSIPIKERSNSSSPSSKQFRPNLEEIKWAFEKFDSNKDGKISFEEYKE 69
           S S K  +K  SF       S  +S   + F+P  EE+KW F+KFD+N+DGK+S EEYK 
Sbjct: 18  SSSRKNVAKKNSFV------SRQTSNEGQSFQPTKEEMKWVFQKFDTNRDGKVSLEEYKA 77

Query: 70  AHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGDIESAFKVYD 129
           A R L   + I +AEA K+F+++D+DGDGF+DL EF+E++    G +K  +I++AF+V+D
Sbjct: 78  AARAL--DRAIGEAEAVKAFRVMDIDGDGFIDLNEFMEMFN-GEGRIKETEIKNAFQVFD 137

Query: 130 SNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKLM 182
            NGDGKISAEE+  ++K LGE+ +L ACK+MVKGVD +GDGFID+ EF+++M
Sbjct: 138 LNGDGKISAEELSHVLKRLGESCSLSACKKMVKGVDGNGDGFIDLNEFTRMM 180

BLAST of CSPI02G14340 vs. TrEMBL
Match: G7K7Z2_MEDTR (EF hand calcium-binding family protein OS=Medicago truncatula GN=MTR_5g017550 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 4.5e-38
Identity = 94/181 (51.93%), Postives = 124/181 (68.51%), Query Frame = 1

Query: 3   SLLKLFRSCSGK--RPSKNTSFSIPIKERSNSSSPSSKQFRPNLEEIKWAFEKFDSNKDG 62
           S LK   S S K   P KN +     K R  SS+  S  F+P  +E+KW FEKFD+NKDG
Sbjct: 5   SFLKFHGSFSAKPTSPRKNIAQKNSFKLRETSSTEMS--FQPKKDEMKWVFEKFDTNKDG 64

Query: 63  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 122
           KIS EEYK A + L   K I D +A K+F ++D D DGF+D KEF+E++   + ++K  +
Sbjct: 65  KISLEEYKAAAKSL--DKGIGDPDAVKAFNVMDSDKDGFIDFKEFMEMFNGENNKIKEEE 124

Query: 123 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 182
           I+SAF+V+D NGDGKISAEE+  I K LGE+ +L ACK+MVKGVD DGDG ID+ EF+++
Sbjct: 125 IKSAFQVFDINGDGKISAEELSQIFKRLGESCSLSACKKMVKGVDGDGDGLIDLNEFTRM 181

BLAST of CSPI02G14340 vs. TAIR10
Match: AT2G15680.1 (AT2G15680.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 131.3 bits (329), Expect = 6.2e-31
Identity = 77/177 (43.50%), Postives = 113/177 (63.84%), Query Frame = 1

Query: 6   KLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKQF-RPNLEEIKWAFEKFDSNKDGKISF 65
           KL ++   ++PS+  S     ++ S  SSP    F +P++ E++  F +FD +KDGKIS 
Sbjct: 12  KLSKNKMLRKPSRMFSRD---RQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLDKDGKISQ 71

Query: 66  EEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGDIESA 125
            EYK   R L   + I D    K FK VD+DGDGF+D +EF++ Y  S G ++  DI ++
Sbjct: 72  TEYKVVLRALGQERAIEDVP--KIFKAVDLDGDGFIDFREFIDAYKRSGG-IRSSDIRNS 131

Query: 126 FKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKLM 182
           F  +D NGDGKISAEEVM ++  LGE  +L+ C +MV+ VD DGDG ++++EF K+M
Sbjct: 132 FWTFDLNGDGKISAEEVMSVLWKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKMM 182

BLAST of CSPI02G14340 vs. TAIR10
Match: AT5G37770.1 (AT5G37770.1 EF hand calcium-binding protein family)

HSP 1 Score: 115.2 bits (287), Expect = 4.6e-26
Identity = 61/144 (42.36%), Postives = 93/144 (64.58%), Query Frame = 1

Query: 43  NLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDL 102
           ++++IK  F++FD N DGKIS +E KE  R L  S   +  E     K  D+DG+GF+DL
Sbjct: 14  SMDDIKKVFQRFDKNGDGKISVDELKEVIRAL--SPTASPEETVTMMKQFDLDGNGFIDL 73

Query: 103 KEFVELYTMSSG-----EVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKAC 162
            EFV L+ +  G        V D++ AF++YD +G+G+ISA+E+  +MK LGE  +++ C
Sbjct: 74  DEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDC 133

Query: 163 KQMVKGVDMDGDGFIDVQEFSKLM 182
           K+M+  VD+DGDG ++  EF K+M
Sbjct: 134 KKMISKVDIDGDGCVNFDEFKKMM 155

BLAST of CSPI02G14340 vs. TAIR10
Match: AT1G66400.1 (AT1G66400.1 calmodulin like 23)

HSP 1 Score: 113.2 bits (282), Expect = 1.7e-25
Identity = 59/142 (41.55%), Postives = 91/142 (64.08%), Query Frame = 1

Query: 43  NLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDL 102
           ++E+IK  F++FD N DGKIS +E K+    L  S   +  E +   K  D+DG+GF+DL
Sbjct: 12  SMEDIKKVFQRFDKNNDGKISIDELKDVIGAL--SPNASQEETKAMMKEFDLDGNGFIDL 71

Query: 103 KEFVELYTMS---SGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQ 162
            EFV L+ +S   S    + D++ AF +YD + +G+ISA E+  +MK LGE  +++ C++
Sbjct: 72  DEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDCQR 131

Query: 163 MVKGVDMDGDGFIDVQEFSKLM 182
           M+  VD DGDG +D +EF K+M
Sbjct: 132 MINKVDSDGDGCVDFEEFKKMM 151

BLAST of CSPI02G14340 vs. TAIR10
Match: AT5G17470.1 (AT5G17470.1 EF hand calcium-binding protein family)

HSP 1 Score: 109.4 bits (272), Expect = 2.5e-24
Identity = 63/143 (44.06%), Postives = 86/143 (60.14%), Query Frame = 1

Query: 51  FEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYT 110
           FE+ D NKDGKIS++E+ EA R  A S  IT  E +  F+ +DVDGD  +D+ E+     
Sbjct: 7   FERVDKNKDGKISWDEFAEAIR--AFSPSITSEEIDNMFREIDVDGDNQIDVAEYASCL- 66

Query: 111 MSSGEVKVGD----IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDM 170
           M  GE    D    ++ AF +YD +GDGKISA E+  ++K LGE  T+  C  MV+ VD 
Sbjct: 67  MLGGEGNKEDEDIVMKEAFDLYDIDGDGKISASEIHVVLKRLGEKQTIAECIAMVRAVDA 126

Query: 171 DGDGFIDVQEFSKLMGKYSSKFQ 190
           DGDGF+  +EF  +M   + K Q
Sbjct: 127 DGDGFVSFEEFKTMMSCNNKKLQ 146

BLAST of CSPI02G14340 vs. TAIR10
Match: AT1G24620.1 (AT1G24620.1 EF hand calcium-binding protein family)

HSP 1 Score: 103.2 bits (256), Expect = 1.8e-22
Identity = 56/142 (39.44%), Postives = 88/142 (61.97%), Query Frame = 1

Query: 41  RPNLEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFV 100
           +  + E++  F+KFD N DGKIS +E       L    E+ + E EK+   +D  GDG++
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLG--HEVPEEELEKAITEIDRKGDGYI 91

Query: 101 DLKEFVELYTMSSGEVKV-GDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQ 160
           + +EFVEL T    +  V  +++ AF VYD +G+G ISAEE+  +++ LG+  ++  C++
Sbjct: 92  NFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRK 151

Query: 161 MVKGVDMDGDGFIDVQEFSKLM 182
           M+ GVD DGDG ID +EF  +M
Sbjct: 152 MIGGVDKDGDGTIDFEEFKIMM 171

BLAST of CSPI02G14340 vs. NCBI nr
Match: gi|449460606|ref|XP_004148036.1| (PREDICTED: probable calcium-binding protein CML32 [Cucumis sativus])

HSP 1 Score: 366.3 bits (939), Expect = 3.2e-98
Identity = 189/190 (99.47%), Postives = 189/190 (99.47%), Query Frame = 1

Query: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKQFRPNLEEIKWAFEKFDSNKDG 60
           MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSK FRPNLEEIKWAFEKFDSNKDG
Sbjct: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDG 60

Query: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120
           KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD
Sbjct: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120

Query: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180
           IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL
Sbjct: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180

Query: 181 MGKYSSKFQN 191
           MGKYSSKFQN
Sbjct: 181 MGKYSSKFQN 190

BLAST of CSPI02G14340 vs. NCBI nr
Match: gi|659115973|ref|XP_008457835.1| (PREDICTED: probable calcium-binding protein CML32 [Cucumis melo])

HSP 1 Score: 347.4 bits (890), Expect = 1.6e-92
Identity = 180/189 (95.24%), Postives = 182/189 (96.30%), Query Frame = 1

Query: 1   MSSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKQFRPNLEEIKWAFEKFDSNKDG 60
           M+SLLKLFRSCSGKRPSKNTSFSI IKERSNSSS SSK FRPNLEEIKWAFEKFDSNKDG
Sbjct: 1   MTSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDG 60

Query: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 120
           KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVD DGDGFVDLKEFVELYTMSSGEVKVGD
Sbjct: 61  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGD 120

Query: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 180
           IESAFKVYDSNGDGKISAEEVMGIMKILGENTTL ACKQMVKGVDMDGDGFID+QEFSKL
Sbjct: 121 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKL 180

Query: 181 MGKYSSKFQ 190
           M  YSSKFQ
Sbjct: 181 MDTYSSKFQ 189

BLAST of CSPI02G14340 vs. NCBI nr
Match: gi|502160872|ref|XP_004511937.1| (PREDICTED: calmodulin-like protein 1 [Cicer arietinum])

HSP 1 Score: 176.4 bits (446), Expect = 4.7e-41
Identity = 100/181 (55.25%), Postives = 129/181 (71.27%), Query Frame = 1

Query: 3   SLLKLFRSCSGKR--PSKNTSFSIPIKERSNSSSPSSKQFRPNLEEIKWAFEKFDSNKDG 62
           S +K   S SGK   P+KN S     K +  +SS  S  F+P  EEIKW FEKFD+NKDG
Sbjct: 5   SFMKFHGSFSGKATSPTKNVSQKNSFKSKQTTSSEMS--FQPQKEEIKWVFEKFDTNKDG 64

Query: 63  KISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGD 122
           KIS EEYK A + L   K I DAEA K+FK +D D DGF+D KEF+E++    G++K  +
Sbjct: 65  KISLEEYKAAAKAL--DKGIGDAEAMKAFKAMDSDKDGFIDFKEFMEMFN-GEGKIKEDE 124

Query: 123 IESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKL 182
           I+SAF+V+D NGDGKISAEE+  I+K LGE+ +L ACK+MVKGVD DGDGFI++ EF+++
Sbjct: 125 IKSAFQVFDINGDGKISAEELSQILKKLGESCSLSACKKMVKGVDGDGDGFINLNEFTRM 180

BLAST of CSPI02G14340 vs. NCBI nr
Match: gi|703106274|ref|XP_010098458.1| (Calmodulin-like protein 1 [Morus notabilis])

HSP 1 Score: 176.0 bits (445), Expect = 6.2e-41
Identity = 85/147 (57.82%), Postives = 113/147 (76.87%), Query Frame = 1

Query: 44  LEEIKWAFEKFDSNKDGKISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLK 103
           +EE++WAFEKFD+N+DGKIS EEYK A R L   +EITD E  K+F  +D DGDGFVD K
Sbjct: 1   MEELRWAFEKFDTNRDGKISREEYKSALRVL--DREITDGEIAKAFNALDTDGDGFVDFK 60

Query: 104 EFVELYTMSSGEVKVGDIESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKG 163
           EFVE++ M SG VK  DIESAF+++D +GDGKISAEE+  ++K LGE+ +++AC+ MVKG
Sbjct: 61  EFVEMFDMGSGGVKASDIESAFRLFDLDGDGKISAEELSQVLKQLGESCSIRACRNMVKG 120

Query: 164 VDMDGDGFIDVQEFSKLMGKYSSKFQN 191
           VD DGDGFI+++EF+++M        N
Sbjct: 121 VDNDGDGFINIEEFTRMMSTTKKPITN 145

BLAST of CSPI02G14340 vs. NCBI nr
Match: gi|470103220|ref|XP_004288041.1| (PREDICTED: calmodulin-like protein 1 [Fragaria vesca subsp. vesca])

HSP 1 Score: 171.4 bits (433), Expect = 1.5e-39
Identity = 95/183 (51.91%), Postives = 124/183 (67.76%), Query Frame = 1

Query: 8   FRSCSGKRPSKNT---SFSIPIKERSNSSSPSSKQFRPNLEEIKWAFEKFDSNKDGKISF 67
           F   S  R S+N      S   KER  SS  S    +P +EEI+W F+KFD+NKDGKIS 
Sbjct: 9   FHYGSSMRKSRNKPAPQLSNVSKERQRSSVSSRSSSQPKMEEIRWVFDKFDTNKDGKISR 68

Query: 68  EEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGDIESA 127
           EEYK   + L   KE+T+AE  K+FK +D DGD  +   EFVE++ M + EVK  DIE A
Sbjct: 69  EEYKSVLKTL--DKEMTEAEIAKTFKALDSDGDDSIGWTEFVEMFNMGADEVKKADIEGA 128

Query: 128 FKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKLMGKY 187
           F+V+D +G+GKISAEE+  I+K LGEN +L ACK+MVKGVD+DGDG ID+ EF+K+MG +
Sbjct: 129 FRVFDLDGNGKISAEELSLILKKLGENCSLGACKKMVKGVDIDGDGLIDLNEFTKMMGSF 188

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CML1_ARATH1.1e-2943.50Calmodulin-like protein 1 OS=Arabidopsis thaliana GN=CML1 PE=2 SV=1[more]
CML24_ARATH8.1e-2542.36Calcium-binding protein CML24 OS=Arabidopsis thaliana GN=CML24 PE=2 SV=2[more]
CML23_ARATH3.1e-2441.55Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=... [more]
CML32_ARATH4.4e-2344.06Probable calcium-binding protein CML32 OS=Arabidopsis thaliana GN=CML32 PE=2 SV=... [more]
CML25_ARATH3.2e-2139.44Probable calcium-binding protein CML25 OS=Arabidopsis thaliana GN=CML25 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0LMP3_CUCSA2.2e-9899.47Uncharacterized protein OS=Cucumis sativus GN=Csa_2G286450 PE=4 SV=1[more]
W9RW36_9ROSA4.3e-4157.82Calmodulin-like protein 1 OS=Morus notabilis GN=L484_002706 PE=4 SV=1[more]
I1LHF1_SOYBN4.5e-3850.58Uncharacterized protein OS=Glycine max GN=GLYMA_11G056500 PE=4 SV=1[more]
A0A0B2SA84_GLYSO4.5e-3850.58Calmodulin-like protein 1 OS=Glycine soja GN=glysoja_003815 PE=4 SV=1[more]
G7K7Z2_MEDTR4.5e-3851.93EF hand calcium-binding family protein OS=Medicago truncatula GN=MTR_5g017550 PE... [more]
Match NameE-valueIdentityDescription
AT2G15680.16.2e-3143.50 Calcium-binding EF-hand family protein[more]
AT5G37770.14.6e-2642.36 EF hand calcium-binding protein family[more]
AT1G66400.11.7e-2541.55 calmodulin like 23[more]
AT5G17470.12.5e-2444.06 EF hand calcium-binding protein family[more]
AT1G24620.11.8e-2239.44 EF hand calcium-binding protein family[more]
Match NameE-valueIdentityDescription
gi|449460606|ref|XP_004148036.1|3.2e-9899.47PREDICTED: probable calcium-binding protein CML32 [Cucumis sativus][more]
gi|659115973|ref|XP_008457835.1|1.6e-9295.24PREDICTED: probable calcium-binding protein CML32 [Cucumis melo][more]
gi|502160872|ref|XP_004511937.1|4.7e-4155.25PREDICTED: calmodulin-like protein 1 [Cicer arietinum][more]
gi|703106274|ref|XP_010098458.1|6.2e-4157.82Calmodulin-like protein 1 [Morus notabilis][more]
gi|470103220|ref|XP_004288041.1|1.5e-3951.91PREDICTED: calmodulin-like protein 1 [Fragaria vesca subsp. vesca][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G14340.1CSPI02G14340.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 121..181
score: 8.4E-10coord: 45..109
score: 1.6
IPR002048EF-hand domainSMARTSM00054efh_1coord: 156..184
score: 0.17coord: 46..74
score: 1.7E-6coord: 84..112
score: 1.1E-4coord: 120..148
score: 1.
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 152..187
score: 12.114coord: 42..77
score: 15.713coord: 80..115
score: 13.872coord: 116..151
score: 15
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 133..184
score: 1.2E-18coord: 44..132
score: 9.5
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 44..183
score: 1.53
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 93..105
score: -coord: 55..67
score: -coord: 129..141
score: -coord: 165..177
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 39..181
score: 4.0
NoneNo IPR availablePANTHERPTHR10891:SF533SUBFAMILY NOT NAMEDcoord: 39..181
score: 4.0

The following gene(s) are paralogous to this gene:

None