CSPI01G25130 (gene) Wild cucumber (PI 183967)

NameCSPI01G25130
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionWAT1-related protein
LocationChr1 : 20552083 .. 20554405 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCTTTGGGCAGACATCATATTGTTAGAAGAGTAAAAATGAAGCCGGTTGTGGTAATGCTTTCAATAAATCTTGCACTTGCTGGTGTGAACATACTACTTAAGGAGATTCTTAATGAAGGGATCAGTCAGCTACTCATTGTCGTTTATCGACAAACGATCTCCTCGCTTTTCTTGTTGCCAATTGCCTATTTTTGGGAAAGGTAAAGTCACCTTAACTTCTTGAAATTTTAGTTTATATTGGTTCCTTTGCCATACTAGAGCCTGCATTTTCCTTCTTACCTTGTCGAAGTTTTGATTTGTTGTATGAAAATGTTTTAAATGCTGCAAAGCTATAAAGAGTTCTCATCGTGTTTGGTGAAAATGCAGGAAAACTAGACCTCAGCTTACTGCTTACATCTTATTCCTCCTTTTCATCAGTTCCTTACTTGGGTTAGTAATTAGTATAGACATTTTTTTGTTAATTGATCCGTGTATAACTACCTTTTAAAGAATTGCATATATAGCAAAATTTATTTGTGCGATAATTTATTTTACAGCTGAATCTAAATTTTGCTATATTTGTAAAAAATTTTACATCGTGCTATATATGCTAATACTTTGAGCCTAAGAACTTTATGATGTTGTTTTAGTTAGTAAGAAACTTTGGAATGATGTACTAATGCAGGTTAACACTAACATTATACCTATTCCTAATTGGTCTACACTTCACCTCTGCAACTTTCTCTTGTGCATTTCTCAACCTAGTACCTGTAAACACGTTCATTCTAGCAGTATTGTTCCGGTACGTGCCGAAAGAACTGCAGTTCATTTTCTTTCCTAACATTGACAAAAATGTGATGAAAGTAACTGGTTTCAAAATATTTCTAGTATGGAAAAAGTGAACATGAAAAGCAAGGGTGGAATTGCCAAAGTGGTAGGGACACTGGTGTGCATTTCAGGAAACTTACTATTGATTCTTTACAAAGGAACACCACTAACGAGCCCTGGATCGACAAATGGGAAAACAAACGGAGGTCGTGCAACGGTTGAAGCTTATACACAAGGAAAATGGTTAATAGGTTCATTGGTTTTGACTGGAGGGTGTTTTATGTGGTCATCTTGGTTCCTTATGCAAACAAGAGTTGGGAGGGTTTATCCGTGTCAATATTCTAGTACTTGTATTATGTCATTTTTCAGTGCCCTTCAATCAGCTGTTTTGCACTTGATTATAGACAGGAAGAACTCACTGTTCATTGTGAAAGGGAAATTTGCTATCTTAAGTCTCATATATGCTGTAAGTATCAGTTTTTTCTTTTTGCCAAGTTAAGTTGCTATTTTGTTGCTAAAGTTTGTTTGTGTTTTGTGATGGGTTCAATAGGGATCAGTGGGATCAGGGTTGTGTTATGTGGGAATGTCTTGGTGTGTAAAGCAAAAAGGGCCAGTTTTCACAGCTGCTTTTACCCCTTTTATGGAAATTTTTGCTGCCATATTTGATTTCTTCATCTTACATGAACAGATTCATCTAGGAAGGTTTGCTTCTTTCTTTCCACTTTCTTTTCCTCCTATTGCTTGATTAAGACTCTCAAATTGTTTTTAACTTTGCTCTCTTGAAGTTGAAGGAAAAACAATGACGCTCCATTAAGTTCTTGACCGCTCTCTTTTTTTCTTACACTACTCTACATCTTTTTTTTCATAAAAAAATATTGATAAAAAGTAGATAACAAAATCAGATATGAAAAGACAGAATAAGTGTTTATAAATTTAATTTTCAAAACACCGAAATGATTATCAAATGAGACCTAATAACTTCTATAAGCATTTCAAATAATATAGAGAAAGATAAATTTTGTTTTCAAGAGTAGGTTAGTTTTACTTATATTGATTATTGTTTGGACGTGATTTTAAAATTGATAAAGATCATATTTTTCATTCTTAAATACGTCTTTAATCACTCAAAATTTATTTATTTTACACTTTTAAACACAATTTTCATACTACTCATACCTCAAAACAAACTAACATCAAAATCAAAACATATACTTTGGTAGAAAGTAGCTAATGATGAAATCTTTTATTATGAAAAATTTTGGCTGAAAAATGTGAGGTTGGTGATGTGGCAGTGTGGTAGGGTCAGTGTTGATCATAAGTGGAATGTACATTCTTCTATGGGGAAAAGACAAAGAAGCAAAGGAATGTGTAGTGAAGCAAACTATATCAATAGAAGAAGCCTATCATAAATCAAACATACCATCAACAAATACAGATGCATTGGAACAAATATAAATGCATTTGCATTTCTTCTAAGGCAGCTTCTTAGTTTTATATTATTGGATGAACCCAAAG

mRNA sequence

ATGAAGCCGGTTGTGGTAATGCTTTCAATAAATCTTGCACTTGCTGGTGTGAACATACTACTTAAGGAGATTCTTAATGAAGGGATCAGTCAGCTACTCATTGTCGTTTATCGACAAACGATCTCCTCGCTTTTCTTGTTGCCAATTGCCTATTTTTGGGAAAGGAAAACTAGACCTCAGCTTACTGCTTACATCTTATTCCTCCTTTTCATCAGTTCCTTACTTGGGTTAACACTAACATTATACCTATTCCTAATTGGTCTACACTTCACCTCTGCAACTTTCTCTTGTGCATTTCTCAACCTAGTACCTGTAAACACGTTCATTCTAGCAGTATTGTTCCGTATGGAAAAAGTGAACATGAAAAGCAAGGGTGGAATTGCCAAAGTGGTAGGGACACTGGTGTGCATTTCAGGAAACTTACTATTGATTCTTTACAAAGGAACACCACTAACGAGCCCTGGATCGACAAATGGGAAAACAAACGGAGGTCGTGCAACGGTTGAAGCTTATACACAAGGAAAATGGTTAATAGGTTCATTGGTTTTGACTGGAGGGTGTTTTATGTGGTCATCTTGGTTCCTTATGCAAACAAGAGTTGGGAGGGTTTATCCGTGTCAATATTCTAGTACTTGTATTATGTCATTTTTCAGTGCCCTTCAATCAGCTGTTTTGCACTTGATTATAGACAGGAAGAACTCACTGTTCATTGTGAAAGGGAAATTTGCTATCTTAAGTCTCATATATGCTGGATCAGTGGGATCAGGGTTGTGTTATGTGGGAATGTCTTGGTGTGTAAAGCAAAAAGGGCCAGTTTTCACAGCTGCTTTTACCCCTTTTATGGAAATTTTTGCTGCCATATTTGATTTCTTCATCTTACATGAACAGATTCATCTAGGAAGTGTGGTAGGGTCAGTGTTGATCATAAGTGGAATGTACATTCTTCTATGGGGAAAAGACAAAGAAGCAAAGGAATGTGTAGTGAAGCAAACTATATCAATAGAAGAAGCCTATCATAAATCAAACATACCATCAACAAATACAGATGCATTGGAACAAATATAA

Coding sequence (CDS)

ATGAAGCCGGTTGTGGTAATGCTTTCAATAAATCTTGCACTTGCTGGTGTGAACATACTACTTAAGGAGATTCTTAATGAAGGGATCAGTCAGCTACTCATTGTCGTTTATCGACAAACGATCTCCTCGCTTTTCTTGTTGCCAATTGCCTATTTTTGGGAAAGGAAAACTAGACCTCAGCTTACTGCTTACATCTTATTCCTCCTTTTCATCAGTTCCTTACTTGGGTTAACACTAACATTATACCTATTCCTAATTGGTCTACACTTCACCTCTGCAACTTTCTCTTGTGCATTTCTCAACCTAGTACCTGTAAACACGTTCATTCTAGCAGTATTGTTCCGTATGGAAAAAGTGAACATGAAAAGCAAGGGTGGAATTGCCAAAGTGGTAGGGACACTGGTGTGCATTTCAGGAAACTTACTATTGATTCTTTACAAAGGAACACCACTAACGAGCCCTGGATCGACAAATGGGAAAACAAACGGAGGTCGTGCAACGGTTGAAGCTTATACACAAGGAAAATGGTTAATAGGTTCATTGGTTTTGACTGGAGGGTGTTTTATGTGGTCATCTTGGTTCCTTATGCAAACAAGAGTTGGGAGGGTTTATCCGTGTCAATATTCTAGTACTTGTATTATGTCATTTTTCAGTGCCCTTCAATCAGCTGTTTTGCACTTGATTATAGACAGGAAGAACTCACTGTTCATTGTGAAAGGGAAATTTGCTATCTTAAGTCTCATATATGCTGGATCAGTGGGATCAGGGTTGTGTTATGTGGGAATGTCTTGGTGTGTAAAGCAAAAAGGGCCAGTTTTCACAGCTGCTTTTACCCCTTTTATGGAAATTTTTGCTGCCATATTTGATTTCTTCATCTTACATGAACAGATTCATCTAGGAAGTGTGGTAGGGTCAGTGTTGATCATAAGTGGAATGTACATTCTTCTATGGGGAAAAGACAAAGAAGCAAAGGAATGTGTAGTGAAGCAAACTATATCAATAGAAGAAGCCTATCATAAATCAAACATACCATCAACAAATACAGATGCATTGGAACAAATATAA
BLAST of CSPI01G25130 vs. Swiss-Prot
Match: WTR24_ARATH (WAT1-related protein At3g30340 OS=Arabidopsis thaliana GN=At3g30340 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 5.4e-91
Identity = 172/329 (52.28%), Postives = 244/329 (74.16%), Query Frame = 1

Query: 2   KPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQL 61
           K V++M  IN+ L+ VN++ K++++EG+++++   YR  + +LFL+P A F ER  RP+L
Sbjct: 10  KAVLMMSMINIGLSVVNVMFKKMIDEGLNRMVATTYRLAVGTLFLIPFAIFLERHNRPKL 69

Query: 62  TAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVNM 121
           T  IL  LF S+LLG +L  Y FLIGL +TS+TFS AF N+VP  TF LA++FR E +N+
Sbjct: 70  TGRILCSLFFSALLGTSLVQYFFLIGLEYTSSTFSLAFSNMVPSVTFALALVFRQETLNI 129

Query: 122 KSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGSL 181
           KS  G AK++GT++CI G L+L LYKGT L+   ST+ +T+    +  A TQ KW +GS+
Sbjct: 130 KSNVGRAKLLGTMICICGALVLTLYKGTALSREHSTHMETHTRTDSTGAMTQ-KWAMGSI 189

Query: 182 VLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKGK 241
           +L     +WSSWF++Q ++ RVYPCQY+ST I+SFF  +QSA+L LI +R  S+++VK K
Sbjct: 190 MLVISIIIWSSWFIVQAKISRVYPCQYTSTTILSFFGVIQSALLSLISERSTSMWVVKDK 249

Query: 242 FAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLGS 301
           F +L+L+Y+G VGSGLCYVGMSWC++Q+G VFT++F P +++FAAIF F  LHEQI+ GS
Sbjct: 250 FQVLALLYSGIVGSGLCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSFLHEQIYCGS 309

Query: 302 VVGSVLIISGMYILLWGKDKEAKECVVKQ 331
           V+GS++II G+YILLWGK K+    V KQ
Sbjct: 310 VIGSMVIIVGLYILLWGKSKDKSASVTKQ 337

BLAST of CSPI01G25130 vs. Swiss-Prot
Match: WTR29_ARATH (WAT1-related protein At4g01440 OS=Arabidopsis thaliana GN=At4g01440 PE=2 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.3e-81
Identity = 160/323 (49.54%), Postives = 224/323 (69.35%), Query Frame = 1

Query: 3   PVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLT 62
           PV++M+ IN AL   N L+K++L+ G++ ++I  YR  IS+LFL PIA+FWERKTRP LT
Sbjct: 10  PVIIMVMINSALGLANALVKKVLDGGVNHMVIATYRLAISTLFLAPIAFFWERKTRPTLT 69

Query: 63  AYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVNMK 122
             IL  LF S+L+G +LT Y FL+GL +TSAT +CAF+++ P  TF++A++FR+EK+NMK
Sbjct: 70  LNILVQLFFSALVGASLTQYFFLLGLSYTSATLACAFISMTPAITFVMALIFRVEKLNMK 129

Query: 123 SKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGSLV 182
           SK G+  V+G L+CI G LLL +YKG PLT       +T+       A     W+IG ++
Sbjct: 130 SKAGMGMVMGALICIGGALLLTMYKGVPLTKLRKL--ETHQLINNNHAMKPENWIIGCVL 189

Query: 183 LTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKGKF 242
           L  G   + SW L+Q +V   YPCQYSST ++SFF  +Q A+L LI  R  + +I+  K 
Sbjct: 190 LFAGSSCFGSWMLIQAKVNEKYPCQYSSTVVLSFFGTIQCALLSLIKSRDITAWILTDKL 249

Query: 243 AILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLGSV 302
            I++++YAG+V  G+C VG SWC++++GP+FT+ FTP   IFA +FDF ILH QI LGSV
Sbjct: 250 DIVTIVYAGAVAQGICTVGTSWCIRKRGPIFTSIFTPVGLIFATLFDFLILHRQIFLGSV 309

Query: 303 VGSVLIISGMYILLWGKDKEAKE 326
           VGS ++I G+YI L GK +  KE
Sbjct: 310 VGSGVVIFGLYIFLLGKVRLMKE 330

BLAST of CSPI01G25130 vs. Swiss-Prot
Match: WTR30_ARATH (WAT1-related protein At4g01450 OS=Arabidopsis thaliana GN=At4g01450 PE=2 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 1.9e-72
Identity = 147/351 (41.88%), Postives = 226/351 (64.39%), Query Frame = 1

Query: 3   PVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLT 62
           P++V++  N+    VN L+K++L+ GI+ ++I  YR  IS+LFLLP+AYFWERKTRP+LT
Sbjct: 10  PMIVLIVSNMIAGMVNALVKKVLDGGINHMVIATYRLGISTLFLLPVAYFWERKTRPKLT 69

Query: 63  AYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVNMK 122
             I   LF+S+L G +L  Y +L+GL +TSAT   AF  ++P  TF++A++F  EK+++K
Sbjct: 70  LSISCQLFVSALFGASLMQYFYLLGLSYTSATLGSAFWAIMPSLTFVMALIFGFEKLSLK 129

Query: 123 SKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGSLV 182
           +K G   V+GTL+ + G LLL +Y+G PLT+       +N             W+ G   
Sbjct: 130 TKIGYGVVLGTLISLVGGLLLTMYQGIPLTNSPEQAANSNNHTG------HENWIKGCFF 189

Query: 183 LTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKGKF 242
           L  G  ++SSW L+Q ++   YPC YSST I+S F  LQ A+L LI  R    +I++ + 
Sbjct: 190 LLTGVVLFSSWMLIQAKINVKYPCPYSSTVILSVFGTLQCALLSLIKTRHLEDWILRDEL 249

Query: 243 AILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLGSV 302
            I++++ AG V  G+C VGMSWC+KQ+GPV +++F+P + + A +FDF ILH +I+LGSV
Sbjct: 250 TIITVVIAGVVAQGMCTVGMSWCIKQQGPVVSSSFSPVVLMSATVFDFLILHREIYLGSV 309

Query: 303 VGSVLIISGMYILLWGKDKEAKECVVKQ--TISIEEAYHKSNIPSTNTDAL 352
           +GSV+++ G+YI LW + K+  EC + +  T ++EE   +    + N   L
Sbjct: 310 IGSVVVVIGLYIFLWSRSKQIVECKIMKLPTNTVEEEKEEEGRTNVNNGQL 354

BLAST of CSPI01G25130 vs. Swiss-Prot
Match: WTR28_ARATH (WAT1-related protein At4g01430 OS=Arabidopsis thaliana GN=At4g01430 PE=2 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 8.4e-68
Identity = 150/347 (43.23%), Postives = 216/347 (62.25%), Query Frame = 1

Query: 3   PVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLT 62
           PV+VML  ++A+  VN L+K+ L+ G++ ++   YR  IS+L L+P +Y WERKTRPQLT
Sbjct: 9   PVIVMLISSVAMGSVNALVKKALDVGVNHMIFGAYRMAISALILVPFSYIWERKTRPQLT 68

Query: 63  AYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKV-NM 122
             +L   FIS LLG +L  + FL+GL +TSAT S A ++++P  TF LA++FR+E   N+
Sbjct: 69  FMLLCEHFISGLLGASLMQFFFLLGLSYTSATVSMALVSMLPAITFALALIFRIENAQNL 128

Query: 123 KSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGST-----NGKTNGGRATVEAYTQGKW 182
           KSK G+ KV+GTL+CI G +LL  YKG  L++P S      N   N G    +     KW
Sbjct: 129 KSKAGVLKVMGTLICIMGAMLLTFYKGPELSNPHSHPQARHNNNNNNGHDQTK-----KW 188

Query: 183 LIGSLVLTGGCFMWSSWFLMQTRVGRVYPC-QYSSTCIMSFFSALQSAVLHLIIDRKNSL 242
           L+G L L  G  + S W L Q ++   YP  +YSSTC+MS F++ Q A+L L   R    
Sbjct: 189 LLGCLYLVIGTVLLSLWMLFQGKLSFKYPGNKYSSTCLMSVFASFQCAILSLYKSRDVKD 248

Query: 243 FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE 302
           +I++ KF IL  +YAG VG  +  V  SW +K  G VF + F+P   + A +FDF ILH 
Sbjct: 249 WIIEDKFVILVTLYAGIVGQAMSTVVTSWSIKMTGAVFVSTFSPVSLVAATLFDFLILHS 308

Query: 303 QIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSN 343
            ++LGS++GSV+ I+G+Y+ LWG+  E  + V K   S + + +K N
Sbjct: 309 PLYLGSILGSVVTITGLYVFLWGRKNETDQSVSKTLNSSQFSQNKDN 350

BLAST of CSPI01G25130 vs. Swiss-Prot
Match: WTR1_ARATH (WAT1-related protein At1g01070 OS=Arabidopsis thaliana GN=At1g01070 PE=2 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 1.4e-67
Identity = 148/351 (42.17%), Postives = 223/351 (63.53%), Query Frame = 1

Query: 3   PVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLT 62
           PV+VM+  N+A+  VN L+K+ L+ G++ ++I  YR  IS+L L+P AY  ERKTRPQ+T
Sbjct: 16  PVIVMVMSNVAMGSVNALVKKALDVGVNHMVIGAYRMAISALILVPFAYVLERKTRPQIT 75

Query: 63  AYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVN-M 122
             ++   F+S LLG +L  + FL+GL +TSAT SCA ++++P  TF LA++FR E V  +
Sbjct: 76  FRLMVDHFVSGLLGASLMQFFFLLGLSYTSATVSCALVSMLPAITFALALIFRTENVKIL 135

Query: 123 KSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRA--TVEAYTQGKWLIG 182
           K+K G+ KV+GTL+CISG L L  YKG  +++   ++  ++GG +    +      WL+G
Sbjct: 136 KTKAGMLKVIGTLICISGALFLTFYKGPQISN---SHSHSHGGASHNNNDQDKANNWLLG 195

Query: 183 SLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVK 242
            L LT G  + S W L Q  +   YPC+YSSTC+MS F+A Q A+L L   R  + +I+ 
Sbjct: 196 CLYLTIGTVLLSLWMLFQGTLSIKYPCKYSSTCLMSIFAAFQCALLSLYKSRDVNDWIID 255

Query: 243 GKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHL 302
            +F I  +IYAG VG  +  V  +W +K+ G VF +AF P   I A +FDF ILH  ++L
Sbjct: 256 DRFVITVIIYAGVVGQAMTTVATTWGIKKLGAVFASAFFPLTLISATLFDFLILHTPLYL 315

Query: 303 GSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDA 351
           GSV+GS++ I+G+Y+ LWGK+KE +      +    EA + +  P+ + D+
Sbjct: 316 GSVIGSLVTITGLYMFLWGKNKETESSTALSSGMDNEAQYTT--PNKDNDS 361

BLAST of CSPI01G25130 vs. TrEMBL
Match: A0A0A0M167_CUCSA (WAT1-related protein OS=Cucumis sativus GN=Csa_1G538830 PE=3 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 6.5e-160
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 1

Query: 1   MKPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ 60
           MKPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ
Sbjct: 1   MKPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ 60

Query: 61  LTAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVN 120
           LTAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVN
Sbjct: 61  LTAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVN 120

Query: 121 MKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGS 180
           MKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGS
Sbjct: 121 MKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGS 180

Query: 181 LVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKG 240
           LVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKG
Sbjct: 181 LVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKG 240

Query: 241 KFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLG 300
           KFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLG
Sbjct: 241 KFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLG 300

BLAST of CSPI01G25130 vs. TrEMBL
Match: M5XK31_PRUPE (WAT1-related protein OS=Prunus persica GN=PRUPE_ppa007702mg PE=3 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 9.3e-106
Identity = 203/339 (59.88%), Postives = 264/339 (77.88%), Query Frame = 1

Query: 2   KPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQL 61
           KP+V ML IN ALA VN+LLK+ L++G++ L+IV YRQ+IS++FL PIA+FWERK+RP+L
Sbjct: 8   KPIVAMLVINFALAMVNVLLKKTLDQGLNHLIIVTYRQSISAVFLAPIAFFWERKSRPEL 67

Query: 62  TAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVNM 121
           T  I+  LF S+L+G+T T YLFLIGL +TSAT+SCAF+N+VPV TF+LA+ F +EKVN+
Sbjct: 68  TFRIICQLFFSALIGVTFTQYLFLIGLQYTSATYSCAFINMVPVFTFLLALPFGLEKVNI 127

Query: 122 KSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGS--TNGKTNGGRATVEAYTQGKWLIG 181
           KSKGGIAK++G L+CI+G L L LYKG PLT+P S  T    N       A   G+W +G
Sbjct: 128 KSKGGIAKILGALICIAGALSLTLYKGMPLTNPNSQDTTQMQNHVNTMTSAKKTGRWAVG 187

Query: 182 SLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRK--NSLFI 241
           S++L  GC +WSSWFL+Q ++G+ YP QYSST I+SFF A+Q+A+L+LI +R    S++ 
Sbjct: 188 SVLLLVGCLLWSSWFLIQAKIGKSYPFQYSSTAILSFFGAIQAAILYLITERNLTMSMWA 247

Query: 242 VKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQI 301
           +KGK  ILS+ YAG+VGSGLCYVGMSWCVKQKG +FTAAFTP ++IF A+ DF   HEQI
Sbjct: 248 MKGKLEILSVTYAGAVGSGLCYVGMSWCVKQKGALFTAAFTPTIQIFVAMVDFSFFHEQI 307

Query: 302 HLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEE 337
           +LGSVVGSVL+I GMYILLWGK  + K  V+K T + E+
Sbjct: 308 YLGSVVGSVLVIIGMYILLWGKSNDPKLIVIKHTQAAED 346

BLAST of CSPI01G25130 vs. TrEMBL
Match: B9I970_POPTR (WAT1-related protein OS=Populus trichocarpa GN=POPTR_0014s10410g PE=3 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.6e-105
Identity = 203/350 (58.00%), Postives = 269/350 (76.86%), Query Frame = 1

Query: 2   KPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQL 61
           KPVV ML I+ A A VN+LLK+++++G + ++I  YR + S++FL PIAYFWERK RP+L
Sbjct: 8   KPVVGMLVISFAFAIVNLLLKKMIDQGTNNMVIATYRLSSSAIFLAPIAYFWERKGRPKL 67

Query: 62  TAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVNM 121
           TA I   LF+ + +GLTLT YLFL+GL + S TFSCAFLN VPVNTFILA+LFR+EK+NM
Sbjct: 68  TASIFCHLFLGAFVGLTLTQYLFLLGLEYISVTFSCAFLNTVPVNTFILALLFRLEKLNM 127

Query: 122 KSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGSL 181
            SK G AKV+GT +C+ G +LLILYKG PLT+P S    T+     +    +  W+ GS+
Sbjct: 128 SSKAGRAKVLGTFICMGGAVLLILYKGIPLTNPRSEATTTHD--ILISGKKKRSWVAGSI 187

Query: 182 VLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKGK 241
           +   GCFMWS+WFLMQ ++ ++YPCQYSST IMSFF A+QSA L  I+ RK S++I+K K
Sbjct: 188 LSLAGCFMWSAWFLMQAKISKIYPCQYSSTAIMSFFGAIQSAALSSILKRKFSMWILKSK 247

Query: 242 FAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLGS 301
             I+S++ AG +GSGLCYVGMSWC+KQ+GPVFT+AFTPF++IFAA+FDF ILHEQI+LGS
Sbjct: 248 LEIISVLNAGIIGSGLCYVGMSWCIKQRGPVFTSAFTPFIQIFAAMFDFSILHEQIYLGS 307

Query: 302 VVGSVLIISGMYILLWGKDKEAKECVVKQT-ISIEEAYH--KSNIPSTNT 349
           V+GS+++I G+YILLWGK  EA +C  KQ  ++ EE  H  ++ I +TN+
Sbjct: 308 VLGSIVVILGLYILLWGKRTEAVDCGEKQAQLTDEEENHDTEAQISATNS 355

BLAST of CSPI01G25130 vs. TrEMBL
Match: A0A151TEH1_CAJCA (WAT1-related protein OS=Cajanus cajan GN=KK1_011663 PE=3 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 5.1e-104
Identity = 203/328 (61.89%), Postives = 258/328 (78.66%), Query Frame = 1

Query: 2   KPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQL 61
           KPV+VM+ +NLALA VNI LK++LNEG+  L I+ YRQ IS++FL PIA F+ERK +  L
Sbjct: 11  KPVLVMIIVNLALAFVNIFLKKVLNEGLDYLTILTYRQAISAIFLAPIACFYERKRK--L 70

Query: 62  TAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVNM 121
             +I+ LLF+S+L+G+TLT YL+LIGL +TSATFSCAFLN VPV TFILA+   +EKVN+
Sbjct: 71  EGHIICLLFLSALVGVTLTQYLYLIGLEYTSATFSCAFLNTVPVFTFILALPLGIEKVNI 130

Query: 122 KSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGSL 181
           KS    AKV+GT VCI G LLLILYKG PL +    +   + G AT  A    KW+IGSL
Sbjct: 131 KSLSSKAKVLGTFVCIGGALLLILYKGMPLINRQQEH-IADKGTATPSASKLKKWIIGSL 190

Query: 182 VLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKGK 241
           +LT GC +WSSWFL+Q R+ + YPCQYSST I+SFF+A+QSA+L L+IDR N+ +I+KGK
Sbjct: 191 LLTAGCLLWSSWFLIQARISKKYPCQYSSTAILSFFAAIQSAILTLLIDRNNAKWILKGK 250

Query: 242 FAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLGS 301
             IL+++Y+G VGSGLCYV MSWCVKQ+GPVFT+AFTP +++F A+ DF ILHE+I+LGS
Sbjct: 251 LEILTVVYSGLVGSGLCYVAMSWCVKQRGPVFTSAFTPLLQVFVAVLDFSILHEEIYLGS 310

Query: 302 VVGSVLIISGMYILLWGKDKEAKECVVK 330
           V GSVL+ISG YILLWGK KE ++C  K
Sbjct: 311 VAGSVLVISGTYILLWGKSKEEEKCAGK 335

BLAST of CSPI01G25130 vs. TrEMBL
Match: I1L797_SOYBN (WAT1-related protein OS=Glycine max GN=GLYMA_09G284000 PE=3 SV=2)

HSP 1 Score: 385.6 bits (989), Expect = 6.7e-104
Identity = 204/351 (58.12%), Postives = 265/351 (75.50%), Query Frame = 1

Query: 2   KPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQL 61
           KPV+VM+ +NLALA VNI LK++LNEG+  L I+ YRQ IS++FL PIA F+ERK +  L
Sbjct: 11  KPVLVMIIVNLALAFVNIFLKKVLNEGVDYLTILTYRQAISAIFLTPIACFYERKRK--L 70

Query: 62  TAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVNM 121
             +I+ LLF+S+L+G+TLT YL+LIGL +TSATF+CAFLN+VPV TFI+A+   +EKVNM
Sbjct: 71  EGHIICLLFLSALVGVTLTQYLYLIGLEYTSATFACAFLNMVPVFTFIMALPLGIEKVNM 130

Query: 122 KSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGSL 181
           K     AKV+GT VCI G L+LILYKG PL +    +   + G     A    KW+IGSL
Sbjct: 131 KKLSAKAKVLGTFVCIGGALMLILYKGVPLINQQPEH-IADKGTIRSSASKLKKWIIGSL 190

Query: 182 VLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKGK 241
           +LT GCF+WSSWFL+Q  + + YPCQYSST I+SFF+++QSA+L L+IDR N+ +I+KGK
Sbjct: 191 LLTAGCFLWSSWFLIQASISKKYPCQYSSTAILSFFASIQSAILTLVIDRSNAKWILKGK 250

Query: 242 FAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLGS 301
             I++++YAG VGSGLCYV MSWCVKQ+GPVFT+AFTP +++F A+ DF ILHE+I+LGS
Sbjct: 251 LEIMTVVYAGLVGSGLCYVAMSWCVKQRGPVFTSAFTPLLQMFVAVLDFSILHEEIYLGS 310

Query: 302 VVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYH-------KSNIPS 346
           V GSVL+ISG YILLWGK KE ++C VK T   +E           SN+PS
Sbjct: 311 VAGSVLVISGTYILLWGKSKEEEQCAVKGTQESQEDEECKNNLEASSNVPS 358

BLAST of CSPI01G25130 vs. TAIR10
Match: AT3G30340.1 (AT3G30340.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 335.9 bits (860), Expect = 3.1e-92
Identity = 172/329 (52.28%), Postives = 244/329 (74.16%), Query Frame = 1

Query: 2   KPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQL 61
           K V++M  IN+ L+ VN++ K++++EG+++++   YR  + +LFL+P A F ER  RP+L
Sbjct: 10  KAVLMMSMINIGLSVVNVMFKKMIDEGLNRMVATTYRLAVGTLFLIPFAIFLERHNRPKL 69

Query: 62  TAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVNM 121
           T  IL  LF S+LLG +L  Y FLIGL +TS+TFS AF N+VP  TF LA++FR E +N+
Sbjct: 70  TGRILCSLFFSALLGTSLVQYFFLIGLEYTSSTFSLAFSNMVPSVTFALALVFRQETLNI 129

Query: 122 KSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGSL 181
           KS  G AK++GT++CI G L+L LYKGT L+   ST+ +T+    +  A TQ KW +GS+
Sbjct: 130 KSNVGRAKLLGTMICICGALVLTLYKGTALSREHSTHMETHTRTDSTGAMTQ-KWAMGSI 189

Query: 182 VLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKGK 241
           +L     +WSSWF++Q ++ RVYPCQY+ST I+SFF  +QSA+L LI +R  S+++VK K
Sbjct: 190 MLVISIIIWSSWFIVQAKISRVYPCQYTSTTILSFFGVIQSALLSLISERSTSMWVVKDK 249

Query: 242 FAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLGS 301
           F +L+L+Y+G VGSGLCYVGMSWC++Q+G VFT++F P +++FAAIF F  LHEQI+ GS
Sbjct: 250 FQVLALLYSGIVGSGLCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSFLHEQIYCGS 309

Query: 302 VVGSVLIISGMYILLWGKDKEAKECVVKQ 331
           V+GS++II G+YILLWGK K+    V KQ
Sbjct: 310 VIGSMVIIVGLYILLWGKSKDKSASVTKQ 337

BLAST of CSPI01G25130 vs. TAIR10
Match: AT4G01440.1 (AT4G01440.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 303.9 bits (777), Expect = 1.3e-82
Identity = 160/323 (49.54%), Postives = 224/323 (69.35%), Query Frame = 1

Query: 3   PVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLT 62
           PV++M+ IN AL   N L+K++L+ G++ ++I  YR  IS+LFL PIA+FWERKTRP LT
Sbjct: 10  PVIIMVMINSALGLANALVKKVLDGGVNHMVIATYRLAISTLFLAPIAFFWERKTRPTLT 69

Query: 63  AYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVNMK 122
             IL  LF S+L+G +LT Y FL+GL +TSAT +CAF+++ P  TF++A++FR+EK+NMK
Sbjct: 70  LNILVQLFFSALVGASLTQYFFLLGLSYTSATLACAFISMTPAITFVMALIFRVEKLNMK 129

Query: 123 SKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGSLV 182
           SK G+  V+G L+CI G LLL +YKG PLT       +T+       A     W+IG ++
Sbjct: 130 SKAGMGMVMGALICIGGALLLTMYKGVPLTKLRKL--ETHQLINNNHAMKPENWIIGCVL 189

Query: 183 LTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKGKF 242
           L  G   + SW L+Q +V   YPCQYSST ++SFF  +Q A+L LI  R  + +I+  K 
Sbjct: 190 LFAGSSCFGSWMLIQAKVNEKYPCQYSSTVVLSFFGTIQCALLSLIKSRDITAWILTDKL 249

Query: 243 AILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLGSV 302
            I++++YAG+V  G+C VG SWC++++GP+FT+ FTP   IFA +FDF ILH QI LGSV
Sbjct: 250 DIVTIVYAGAVAQGICTVGTSWCIRKRGPIFTSIFTPVGLIFATLFDFLILHRQIFLGSV 309

Query: 303 VGSVLIISGMYILLWGKDKEAKE 326
           VGS ++I G+YI L GK +  KE
Sbjct: 310 VGSGVVIFGLYIFLLGKVRLMKE 330

BLAST of CSPI01G25130 vs. TAIR10
Match: AT4G01450.2 (AT4G01450.2 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 274.2 bits (700), Expect = 1.1e-73
Identity = 147/351 (41.88%), Postives = 226/351 (64.39%), Query Frame = 1

Query: 3   PVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLT 62
           P++V++  N+    VN L+K++L+ GI+ ++I  YR  IS+LFLLP+AYFWERKTRP+LT
Sbjct: 10  PMIVLIVSNMIAGMVNALVKKVLDGGINHMVIATYRLGISTLFLLPVAYFWERKTRPKLT 69

Query: 63  AYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVNMK 122
             I   LF+S+L G +L  Y +L+GL +TSAT   AF  ++P  TF++A++F  EK+++K
Sbjct: 70  LSISCQLFVSALFGASLMQYFYLLGLSYTSATLGSAFWAIMPSLTFVMALIFGFEKLSLK 129

Query: 123 SKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGSLV 182
           +K G   V+GTL+ + G LLL +Y+G PLT+       +N             W+ G   
Sbjct: 130 TKIGYGVVLGTLISLVGGLLLTMYQGIPLTNSPEQAANSNNHTG------HENWIKGCFF 189

Query: 183 LTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKGKF 242
           L  G  ++SSW L+Q ++   YPC YSST I+S F  LQ A+L LI  R    +I++ + 
Sbjct: 190 LLTGVVLFSSWMLIQAKINVKYPCPYSSTVILSVFGTLQCALLSLIKTRHLEDWILRDEL 249

Query: 243 AILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLGSV 302
            I++++ AG V  G+C VGMSWC+KQ+GPV +++F+P + + A +FDF ILH +I+LGSV
Sbjct: 250 TIITVVIAGVVAQGMCTVGMSWCIKQQGPVVSSSFSPVVLMSATVFDFLILHREIYLGSV 309

Query: 303 VGSVLIISGMYILLWGKDKEAKECVVKQ--TISIEEAYHKSNIPSTNTDAL 352
           +GSV+++ G+YI LW + K+  EC + +  T ++EE   +    + N   L
Sbjct: 310 IGSVVVVIGLYIFLWSRSKQIVECKIMKLPTNTVEEEKEEEGRTNVNNGQL 354

BLAST of CSPI01G25130 vs. TAIR10
Match: AT4G01430.1 (AT4G01430.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 258.8 bits (660), Expect = 4.8e-69
Identity = 150/347 (43.23%), Postives = 216/347 (62.25%), Query Frame = 1

Query: 3   PVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLT 62
           PV+VML  ++A+  VN L+K+ L+ G++ ++   YR  IS+L L+P +Y WERKTRPQLT
Sbjct: 9   PVIVMLISSVAMGSVNALVKKALDVGVNHMIFGAYRMAISALILVPFSYIWERKTRPQLT 68

Query: 63  AYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKV-NM 122
             +L   FIS LLG +L  + FL+GL +TSAT S A ++++P  TF LA++FR+E   N+
Sbjct: 69  FMLLCEHFISGLLGASLMQFFFLLGLSYTSATVSMALVSMLPAITFALALIFRIENAQNL 128

Query: 123 KSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGST-----NGKTNGGRATVEAYTQGKW 182
           KSK G+ KV+GTL+CI G +LL  YKG  L++P S      N   N G    +     KW
Sbjct: 129 KSKAGVLKVMGTLICIMGAMLLTFYKGPELSNPHSHPQARHNNNNNNGHDQTK-----KW 188

Query: 183 LIGSLVLTGGCFMWSSWFLMQTRVGRVYPC-QYSSTCIMSFFSALQSAVLHLIIDRKNSL 242
           L+G L L  G  + S W L Q ++   YP  +YSSTC+MS F++ Q A+L L   R    
Sbjct: 189 LLGCLYLVIGTVLLSLWMLFQGKLSFKYPGNKYSSTCLMSVFASFQCAILSLYKSRDVKD 248

Query: 243 FIVKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE 302
           +I++ KF IL  +YAG VG  +  V  SW +K  G VF + F+P   + A +FDF ILH 
Sbjct: 249 WIIEDKFVILVTLYAGIVGQAMSTVVTSWSIKMTGAVFVSTFSPVSLVAATLFDFLILHS 308

Query: 303 QIHLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSN 343
            ++LGS++GSV+ I+G+Y+ LWG+  E  + V K   S + + +K N
Sbjct: 309 PLYLGSILGSVVTITGLYVFLWGRKNETDQSVSKTLNSSQFSQNKDN 350

BLAST of CSPI01G25130 vs. TAIR10
Match: AT1G01070.1 (AT1G01070.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 258.1 bits (658), Expect = 8.1e-69
Identity = 148/351 (42.17%), Postives = 223/351 (63.53%), Query Frame = 1

Query: 3   PVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQLT 62
           PV+VM+  N+A+  VN L+K+ L+ G++ ++I  YR  IS+L L+P AY  ERKTRPQ+T
Sbjct: 16  PVIVMVMSNVAMGSVNALVKKALDVGVNHMVIGAYRMAISALILVPFAYVLERKTRPQIT 75

Query: 63  AYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVN-M 122
             ++   F+S LLG +L  + FL+GL +TSAT SCA ++++P  TF LA++FR E V  +
Sbjct: 76  FRLMVDHFVSGLLGASLMQFFFLLGLSYTSATVSCALVSMLPAITFALALIFRTENVKIL 135

Query: 123 KSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRA--TVEAYTQGKWLIG 182
           K+K G+ KV+GTL+CISG L L  YKG  +++   ++  ++GG +    +      WL+G
Sbjct: 136 KTKAGMLKVIGTLICISGALFLTFYKGPQISN---SHSHSHGGASHNNNDQDKANNWLLG 195

Query: 183 SLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVK 242
            L LT G  + S W L Q  +   YPC+YSSTC+MS F+A Q A+L L   R  + +I+ 
Sbjct: 196 CLYLTIGTVLLSLWMLFQGTLSIKYPCKYSSTCLMSIFAAFQCALLSLYKSRDVNDWIID 255

Query: 243 GKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHL 302
            +F I  +IYAG VG  +  V  +W +K+ G VF +AF P   I A +FDF ILH  ++L
Sbjct: 256 DRFVITVIIYAGVVGQAMTTVATTWGIKKLGAVFASAFFPLTLISATLFDFLILHTPLYL 315

Query: 303 GSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDA 351
           GSV+GS++ I+G+Y+ LWGK+KE +      +    EA + +  P+ + D+
Sbjct: 316 GSVIGSLVTITGLYMFLWGKNKETESSTALSSGMDNEAQYTT--PNKDNDS 361

BLAST of CSPI01G25130 vs. NCBI nr
Match: gi|778661994|ref|XP_011659182.1| (PREDICTED: WAT1-related protein At3g30340 [Cucumis sativus])

HSP 1 Score: 676.0 bits (1743), Expect = 3.5e-191
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 1

Query: 1   MKPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ 60
           MKPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ
Sbjct: 1   MKPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ 60

Query: 61  LTAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVN 120
           LTAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVN
Sbjct: 61  LTAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVN 120

Query: 121 MKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGS 180
           MKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGS
Sbjct: 121 MKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGS 180

Query: 181 LVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKG 240
           LVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKG
Sbjct: 181 LVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKG 240

Query: 241 KFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLG 300
           KFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLG
Sbjct: 241 KFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLG 300

Query: 301 SVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDALEQI 355
           SVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDALEQI
Sbjct: 301 SVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDALEQI 354

BLAST of CSPI01G25130 vs. NCBI nr
Match: gi|659068539|ref|XP_008444961.1| (PREDICTED: WAT1-related protein At3g30340-like [Cucumis melo])

HSP 1 Score: 656.4 bits (1692), Expect = 2.9e-185
Identity = 345/354 (97.46%), Postives = 347/354 (98.02%), Query Frame = 1

Query: 1   MKPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ 60
           MK VVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ
Sbjct: 1   MKAVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ 60

Query: 61  LTAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVN 120
           LTAY LFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTF LAVLFRMEKVN
Sbjct: 61  LTAYTLFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFFLAVLFRMEKVN 120

Query: 121 MKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGS 180
           MKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRA VEA TQGKWLIGS
Sbjct: 121 MKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRAMVEAETQGKWLIGS 180

Query: 181 LVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKG 240
           LVLTGGCFMWSSWFLMQTRVG+VYPCQYSSTCIMSFFSA+QSAVLHLIIDRKNSLFIVKG
Sbjct: 181 LVLTGGCFMWSSWFLMQTRVGKVYPCQYSSTCIMSFFSAIQSAVLHLIIDRKNSLFIVKG 240

Query: 241 KFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLG 300
           KFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHE IHLG
Sbjct: 241 KFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEPIHLG 300

Query: 301 SVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNIPSTNTDALEQI 355
           SVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNI STNTDALEQI
Sbjct: 301 SVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEEAYHKSNISSTNTDALEQI 354

BLAST of CSPI01G25130 vs. NCBI nr
Match: gi|700210840|gb|KGN65936.1| (hypothetical protein Csa_1G538830 [Cucumis sativus])

HSP 1 Score: 571.6 bits (1472), Expect = 9.4e-160
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 1

Query: 1   MKPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ 60
           MKPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ
Sbjct: 1   MKPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQ 60

Query: 61  LTAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVN 120
           LTAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVN
Sbjct: 61  LTAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVN 120

Query: 121 MKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGS 180
           MKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGS
Sbjct: 121 MKSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGS 180

Query: 181 LVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKG 240
           LVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKG
Sbjct: 181 LVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKG 240

Query: 241 KFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLG 300
           KFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLG
Sbjct: 241 KFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLG 300

BLAST of CSPI01G25130 vs. NCBI nr
Match: gi|596001654|ref|XP_007218191.1| (hypothetical protein PRUPE_ppa007702mg [Prunus persica])

HSP 1 Score: 391.7 bits (1005), Expect = 1.3e-105
Identity = 203/339 (59.88%), Postives = 264/339 (77.88%), Query Frame = 1

Query: 2   KPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQL 61
           KP+V ML IN ALA VN+LLK+ L++G++ L+IV YRQ+IS++FL PIA+FWERK+RP+L
Sbjct: 8   KPIVAMLVINFALAMVNVLLKKTLDQGLNHLIIVTYRQSISAVFLAPIAFFWERKSRPEL 67

Query: 62  TAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVNM 121
           T  I+  LF S+L+G+T T YLFLIGL +TSAT+SCAF+N+VPV TF+LA+ F +EKVN+
Sbjct: 68  TFRIICQLFFSALIGVTFTQYLFLIGLQYTSATYSCAFINMVPVFTFLLALPFGLEKVNI 127

Query: 122 KSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGS--TNGKTNGGRATVEAYTQGKWLIG 181
           KSKGGIAK++G L+CI+G L L LYKG PLT+P S  T    N       A   G+W +G
Sbjct: 128 KSKGGIAKILGALICIAGALSLTLYKGMPLTNPNSQDTTQMQNHVNTMTSAKKTGRWAVG 187

Query: 182 SLVLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRK--NSLFI 241
           S++L  GC +WSSWFL+Q ++G+ YP QYSST I+SFF A+Q+A+L+LI +R    S++ 
Sbjct: 188 SVLLLVGCLLWSSWFLIQAKIGKSYPFQYSSTAILSFFGAIQAAILYLITERNLTMSMWA 247

Query: 242 VKGKFAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQI 301
           +KGK  ILS+ YAG+VGSGLCYVGMSWCVKQKG +FTAAFTP ++IF A+ DF   HEQI
Sbjct: 248 MKGKLEILSVTYAGAVGSGLCYVGMSWCVKQKGALFTAAFTPTIQIFVAMVDFSFFHEQI 307

Query: 302 HLGSVVGSVLIISGMYILLWGKDKEAKECVVKQTISIEE 337
           +LGSVVGSVL+I GMYILLWGK  + K  V+K T + E+
Sbjct: 308 YLGSVVGSVLVIIGMYILLWGKSNDPKLIVIKHTQAAED 346

BLAST of CSPI01G25130 vs. NCBI nr
Match: gi|743790808|ref|XP_011039732.1| (PREDICTED: WAT1-related protein At3g30340-like [Populus euphratica])

HSP 1 Score: 391.7 bits (1005), Expect = 1.3e-105
Identity = 203/350 (58.00%), Postives = 268/350 (76.57%), Query Frame = 1

Query: 2   KPVVVMLSINLALAGVNILLKEILNEGISQLLIVVYRQTISSLFLLPIAYFWERKTRPQL 61
           KPVV ML I+ A A VN+LLK+++++G + ++I  YR + S+ FL PIAYFWERK+RP+L
Sbjct: 8   KPVVGMLVISFAFAIVNLLLKKMIDQGTNNMVIATYRLSSSATFLAPIAYFWERKSRPKL 67

Query: 62  TAYILFLLFISSLLGLTLTLYLFLIGLHFTSATFSCAFLNLVPVNTFILAVLFRMEKVNM 121
           TA I   LF+ + +GLTLT YLFL+GL + S TFSCAFLN VPVNTFILA+LFR+EK+NM
Sbjct: 68  TASIFCHLFLGAFIGLTLTQYLFLLGLEYISVTFSCAFLNTVPVNTFILALLFRLEKLNM 127

Query: 122 KSKGGIAKVVGTLVCISGNLLLILYKGTPLTSPGSTNGKTNGGRATVEAYTQGKWLIGSL 181
            SK G AKV+GT +C+ G +LLILYKG PLT+P S    T+     +    +  W+ GS+
Sbjct: 128 SSKAGRAKVLGTFICMGGAVLLILYKGIPLTNPSSEATTTD---ILISGKKKRSWVAGSI 187

Query: 182 VLTGGCFMWSSWFLMQTRVGRVYPCQYSSTCIMSFFSALQSAVLHLIIDRKNSLFIVKGK 241
           +   GCFMWS+WFLMQ ++ ++YPCQYSST IMSFF A+QSA L LI+ RK S++I+K K
Sbjct: 188 LSLAGCFMWSAWFLMQAKISKIYPCQYSSTAIMSFFGAIQSAALSLILKRKFSMWILKSK 247

Query: 242 FAILSLIYAGSVGSGLCYVGMSWCVKQKGPVFTAAFTPFMEIFAAIFDFFILHEQIHLGS 301
             I+S++ AG +GSGLCYVGMSWC+KQ+GPVFT+AFTPF++IFAA+FDF ILHEQI+LGS
Sbjct: 248 LEIISVLNAGIIGSGLCYVGMSWCIKQRGPVFTSAFTPFIQIFAAMFDFSILHEQIYLGS 307

Query: 302 VVGSVLIISGMYILLWGKDKEAKECVVKQT-ISIEEAYH--KSNIPSTNT 349
           V+GS+ +  G+YILLWGK  EA +C  KQ  ++ EE  H  ++ I +TN+
Sbjct: 308 VLGSIAVTLGLYILLWGKRTEAVDCGEKQAQLTDEEENHDTEAQISATNS 354

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
WTR24_ARATH5.4e-9152.28WAT1-related protein At3g30340 OS=Arabidopsis thaliana GN=At3g30340 PE=2 SV=1[more]
WTR29_ARATH2.3e-8149.54WAT1-related protein At4g01440 OS=Arabidopsis thaliana GN=At4g01440 PE=2 SV=1[more]
WTR30_ARATH1.9e-7241.88WAT1-related protein At4g01450 OS=Arabidopsis thaliana GN=At4g01450 PE=2 SV=1[more]
WTR28_ARATH8.4e-6843.23WAT1-related protein At4g01430 OS=Arabidopsis thaliana GN=At4g01430 PE=2 SV=1[more]
WTR1_ARATH1.4e-6742.17WAT1-related protein At1g01070 OS=Arabidopsis thaliana GN=At1g01070 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0M167_CUCSA6.5e-160100.00WAT1-related protein OS=Cucumis sativus GN=Csa_1G538830 PE=3 SV=1[more]
M5XK31_PRUPE9.3e-10659.88WAT1-related protein OS=Prunus persica GN=PRUPE_ppa007702mg PE=3 SV=1[more]
B9I970_POPTR1.6e-10558.00WAT1-related protein OS=Populus trichocarpa GN=POPTR_0014s10410g PE=3 SV=1[more]
A0A151TEH1_CAJCA5.1e-10461.89WAT1-related protein OS=Cajanus cajan GN=KK1_011663 PE=3 SV=1[more]
I1L797_SOYBN6.7e-10458.12WAT1-related protein OS=Glycine max GN=GLYMA_09G284000 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
AT3G30340.13.1e-9252.28 nodulin MtN21 /EamA-like transporter family protein[more]
AT4G01440.11.3e-8249.54 nodulin MtN21 /EamA-like transporter family protein[more]
AT4G01450.21.1e-7341.88 nodulin MtN21 /EamA-like transporter family protein[more]
AT4G01430.14.8e-6943.23 nodulin MtN21 /EamA-like transporter family protein[more]
AT1G01070.18.1e-6942.17 nodulin MtN21 /EamA-like transporter family protein[more]
Match NameE-valueIdentityDescription
gi|778661994|ref|XP_011659182.1|3.5e-191100.00PREDICTED: WAT1-related protein At3g30340 [Cucumis sativus][more]
gi|659068539|ref|XP_008444961.1|2.9e-18597.46PREDICTED: WAT1-related protein At3g30340-like [Cucumis melo][more]
gi|700210840|gb|KGN65936.1|9.4e-160100.00hypothetical protein Csa_1G538830 [Cucumis sativus][more]
gi|596001654|ref|XP_007218191.1|1.3e-10559.88hypothetical protein PRUPE_ppa007702mg [Prunus persica][more]
gi|743790808|ref|XP_011039732.1|1.3e-10558.00PREDICTED: WAT1-related protein At3g30340-like [Populus euphratica][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000620EamA_dom
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: Molecular Function
TermDefinition
GO:0022857transmembrane transporter activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI01G25130.1CSPI01G25130.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 178..316
score: 7.1E-13coord: 8..137
score: 6.9
NoneNo IPR availablePANTHERPTHR31218:SF10SUBFAMILY NOT NAMEDcoord: 1..354
score: 8.6E
NoneNo IPR availableunknownSSF103481Multidrug resistance efflux transporter EmrEcoord: 37..145
score: 3.14E-8coord: 241..321
score: 6.93

The following gene(s) are paralogous to this gene:

None