Csa4G285710.2 (mRNA) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa4G285710.2
Type	mRNA
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	E3 ubiquitin ligase-like protein; contains IPR004162 (Seven-in-absentia protein, sina), IPR013083 (Zinc finger, RING/FYVE/PHD-type)
Location	Chr4 : 10980832 .. 10982635 (-)
Sequence length	981

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTTTCTATTATTATCCTATTTGCATTGCACATTTGAAAATAAATATCATTGAGTGTGAAGTTTAAATGGAATCCACATAGCTGAAATGATCATCCTACTAAATTGATTGGTCCAGGGAACAAGTTTTGAGATGCTTGCTTATCATAAGTTTCTACGGAACCAATTATGAGTTCTTCAAACAAGTCCCTCTAGCAATGACACTTCTTCTTTTTTAATTAAATCTCAGAGATGATTGTATGAGTACCATAATAGAATTCAGAGAGAAGGGGATGTATCAGCAACCCAAGTCAAGGGAAGAACATGCTTCTTCTGCAGCCAGCTTCTGCAAAGAAGTGACCGAGTTGCGCCTTACATCGATAGATTCTCAACCAGCAACTTCTCGTCCTGAGTTCAAAGGTGTCACTTTCAGGAAAGCTTCAACTGGTGGCTTTGCTAGGAGTCGTAATGATGTACACGAGCTACTTGATTGTACTGTTTGCATGAATTTAATGCATCCTCCAATTTACCAGGTATCGTATTCTTATTGTGATGACTTTCAAAACATATAACGCTTTGCTCATTGATTATTCTGAAAAAAAAAACGTTTCATAGATAAATAAATTCATCCAATAAATAGAACACTGACGCATTTAATTTGGTTGAAGGCCTAGAACCCTAATGGATTATTGAGTCACTCTTTTGTCCCCGGAAATAGCTTTCGGACCAAGTATCAAAAGGACTCTATATAACTTCACTTTCTGTTCAAATGGAAGGCTAGGAATTTTGTTTAAGCGTTGATTTGCATTATGATTGAGTTATTACATTTGAGGTAACTCCAATGCATAACAGCTAACGTAATTTTACGGTTCAGTGTGCAAATGGCCACACTCTTTGTTCAAGCTGCAAGGCAAGGGTTCAGAATTGCTGCCCTACCTGTCGGCATGAGCTTGGGAACATAAGGTGCCTAGCACTGGAAAAGGTGGCCGAGTCCCTTGAGCTCCCTTGTGTGTACCAAAACCTAGGTTGCATTGACATGTTCCCATACTACAGCAAGCTTAAACACGAGAAGAATTGTAAATACCGGCCGTACAACTGCCCTTACGCTGGAGGTGAATGTCATGTAACTGGTGACATTCCATCCCTTGTCATGCATCTCAAGGATGATCACGAGGTGGATATGCATGATGGATGTTCCTTCAGTCATAGATATGTCAAATCCAATCCCCAAGAAGTTGAAAATGCCACATGGATGCTAACTGTAAGATGTCTCTCTTTATTTCAGATTCTTAGGAATAATGAAAGCAAAGAAGAAGCAATTCCATGTAATGAACATCCTCCTGATTTCTCAATTGATCCAATTCAGGTTTTCAACTGCTTTGGACGACAATTCTGTTTGCACTTTGAAGCATTTCGCCTAGGAACAGCACCTGTTTACATGGCATTCCTCCGGTTCATGGGTGACGACAGCGAGGCAAAACAGTTCTCATACAGTCTTGAAGTTGGTGGAAATGGAAGAAAACTCATCTGGCAAGGTATTCCAAGAAGTATTCGCGACAGCCATCGGAAGGTTCGAGACAGTCATGATGGACTAATTATTCAGAGGAAATTGGCATTATTTTGCTCTGGAAACGAGGGGATGGAACTGAAGTTAAAGGTTACAGGACGAATATGGAGAGTGAGGTGACAACAACAAATGAATATTCATCTATCAAACTCAAAACTTGTACAATATGTTGTGACATTGTGACCTTTGTACATGCCTCGGCCCTCCTACAATTCAGCCTTTTTTTAGAACCTCTTATTTCTTCATCACCTCTACATTT

mRNA sequence

ATGAGTACCATAATAGAATTCAGAGAGAAGGGGATGTATCAGCAACCCAAGTCAAGGGAAGAACATGCTTCTTCTGCAGCCAGCTTCTGCAAAGAAGTGACCGAGTTGCGCCTTACATCGATAGATTCTCAACCAGCAACTTCTCGTCCTGAGTTCAAAGGTGTCACTTTCAGGAAAGCTTCAACTGGTGGCTTTGCTAGGAGTCGTAATGATGTACACGAGCTACTTGATTGTACTGTTTGCATGAATTTAATGCATCCTCCAATTTACCAGTGTGCAAATGGCCACACTCTTTGTTCAAGCTGCAAGGCAAGGGTTCAGAATTGCTGCCCTACCTGTCGGCATGAGCTTGGGAACATAAGGTGCCTAGCACTGGAAAAGGTGGCCGAGTCCCTTGAGCTCCCTTGTGTGTACCAAAACCTAGGTTGCATTGACATGTTCCCATACTACAGCAAGCTTAAACACGAGAAGAATTGTAAATACCGGCCGTACAACTGCCCTTACGCTGGAGGTGAATGTCATGTAACTGGTGACATTCCATCCCTTGTCATGCATCTCAAGGATGATCACGAGGTGGATATGCATGATGGATGTTCCTTCAGTCATAGATATGTCAAATCCAATCCCCAAGAAGTTGAAAATGCCACATGGATGCTAACTGTTTTCAACTGCTTTGGACGACAATTCTGTTTGCACTTTGAAGCATTTCGCCTAGGAACAGCACCTGTTTACATGGCATTCCTCCGGTTCATGGGTGACGACAGCGAGGCAAAACAGTTCTCATACAGTCTTGAAGTTGGTGGAAATGGAAGAAAACTCATCTGGCAAGGTATTCCAAGAAGTATTCGCGACAGCCATCGGAAGGTTCGAGACAGTCATGATGGACTAATTATTCAGAGGAAATTGGCATTATTTTGCTCTGGAAACGAGGGGATGGAACTGAAGTTAAAGGTTACAGGACGAATATGGAGAGTGAGGTGA

Coding sequence (CDS)

ATGAGTACCATAATAGAATTCAGAGAGAAGGGGATGTATCAGCAACCCAAGTCAAGGGAAGAACATGCTTCTTCTGCAGCCAGCTTCTGCAAAGAAGTGACCGAGTTGCGCCTTACATCGATAGATTCTCAACCAGCAACTTCTCGTCCTGAGTTCAAAGGTGTCACTTTCAGGAAAGCTTCAACTGGTGGCTTTGCTAGGAGTCGTAATGATGTACACGAGCTACTTGATTGTACTGTTTGCATGAATTTAATGCATCCTCCAATTTACCAGTGTGCAAATGGCCACACTCTTTGTTCAAGCTGCAAGGCAAGGGTTCAGAATTGCTGCCCTACCTGTCGGCATGAGCTTGGGAACATAAGGTGCCTAGCACTGGAAAAGGTGGCCGAGTCCCTTGAGCTCCCTTGTGTGTACCAAAACCTAGGTTGCATTGACATGTTCCCATACTACAGCAAGCTTAAACACGAGAAGAATTGTAAATACCGGCCGTACAACTGCCCTTACGCTGGAGGTGAATGTCATGTAACTGGTGACATTCCATCCCTTGTCATGCATCTCAAGGATGATCACGAGGTGGATATGCATGATGGATGTTCCTTCAGTCATAGATATGTCAAATCCAATCCCCAAGAAGTTGAAAATGCCACATGGATGCTAACTGTTTTCAACTGCTTTGGACGACAATTCTGTTTGCACTTTGAAGCATTTCGCCTAGGAACAGCACCTGTTTACATGGCATTCCTCCGGTTCATGGGTGACGACAGCGAGGCAAAACAGTTCTCATACAGTCTTGAAGTTGGTGGAAATGGAAGAAAACTCATCTGGCAAGGTATTCCAAGAAGTATTCGCGACAGCCATCGGAAGGTTCGAGACAGTCATGATGGACTAATTATTCAGAGGAAATTGGCATTATTTTGCTCTGGAAACGAGGGGATGGAACTGAAGTTAAAGGTTACAGGACGAATATGGAGAGTGAGGTGA

Protein sequence

MSTIIEFREKGMYQQPKSREEHASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKASTGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVTGRIWRVR*

Homology

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BLAST of Csa4G285710.2 vs. Swiss-Prot
Match: SINA2_ARATH (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 3.8e-131
Identity = 219/301 (72.76%), Postives = 249/301 (82.72%), Query Frame = 1


Query: 28  SFCKEVTELRLTSIDSQPATSRPEFKGVTFRKASTGGFAR----SRNDVHELLDCTVCMN 87
           S  KEV E   T +D +  T++ E       K  + G  +    S N V+ELL+C VC N
Sbjct: 6   SALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTN 65

Query: 88  LMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQNLGC 147
           LM+PPI+QC NGHTLCS+CK RVQN CPTCR+ELGNIRCLALEKVAESLE+PC YQNLGC
Sbjct: 66  LMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGC 125

Query: 148 IDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCSFSHR 207
            D+FPYYSKLKHE++C++RPY CPYAG EC VTGDIP+LV+HLKDDH+VDMHDGC+F+HR
Sbjct: 126 HDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHR 185

Query: 208 YVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYS 267
           YVKSNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD++EAK+FSYS
Sbjct: 186 YVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYS 245

Query: 268 LEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVTGRIW 325
           LEVG +GRKL WQGIPRSIRDSHRKVRDS DGLII R LAL+ SG +  ELKL+VTGRIW
Sbjct: 246 LEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIW 305

[Close]

BLAST of Csa4G285710.2 vs. Swiss-Prot
Match: SINA1_ARATH (Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana GN=SINAT1 PE=3 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 3.9e-128
Identity = 216/298 (72.48%), Postives = 245/298 (82.21%), Query Frame = 1


Query: 28  SFCKEVTELRLTSIDSQPATSRPEFKGVTFRKASTG-GFARSRNDVHELLDCTVCMNLMH 87
           S  KE  E   T +D +   ++ E      +  S   G   S N V+ELL+C VC NLM+
Sbjct: 6   SALKEALESNSTGVDYEVKMAKVEANSKPTKSGSGSIGKFHSSNGVYELLECPVCTNLMY 65

Query: 88  PPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQNLGCIDM 147
           PPI+QC NGHTLCSSCK RVQN CPTCR+ELGNIRCLALEKVAESLE+PC YQNLGC D+
Sbjct: 66  PPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQDI 125

Query: 148 FPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCSFSHRYVK 207
           FPYYSKLKHE++C++R Y+CPYAG EC VTGDIP+LV HLKDDH+VDMHDGC+F+HRYVK
Sbjct: 126 FPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRYVK 185

Query: 208 SNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLEV 267
           SNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD++EAK+FSYSLEV
Sbjct: 186 SNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEV 245

Query: 268 GGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVTGRIWR 325
           G + RKL WQGIPRSIRDSHRKVRDS DGLII R LAL+ SG++  ELKL+VTGRIW+
Sbjct: 246 GAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWK 303

[Close]

BLAST of Csa4G285710.2 vs. Swiss-Prot
Match: SINA3_ARATH (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana GN=SINAT3 PE=2 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 9.4e-122
Identity = 197/265 (74.34%), Postives = 227/265 (85.66%), Query Frame = 1


Query: 60  ASTGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN 119
           A+  G   +   VHELL+C VC N M+PPI+QC NGHTLCS+CKARV N CPTCR ELG+
Sbjct: 45  AAASGLLPTTTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGD 104

Query: 120 IRCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDI 179
           IRCLALEKVAESLELPC + +LGC ++FPYYSKLKHE  C +RPY+CPYAG EC VTGDI
Sbjct: 105 IRCLALEKVAESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDI 164

Query: 180 PSLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 239
           P LV HL+DDH+VDMH GC+F+HRYVKSNP+EVENATWMLTVF+CFG+ FCLHFEAF+LG
Sbjct: 165 PFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLG 224

Query: 240 TAPVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQ 299
            APVYMAFLRFMGD++EA+ ++YSLEVGG GRKLIW+G PRS+RDSHRKVRDSHDGLIIQ
Sbjct: 225 MAPVYMAFLRFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQ 284

Query: 300 RKLALFCSGNEGMELKLKVTGRIWR 325
           R +ALF SG +  ELKL+VTGRIW+
Sbjct: 285 RNMALFFSGGDRKELKLRVTGRIWK 309

[Close]

BLAST of Csa4G285710.2 vs. Swiss-Prot
Match: SINA5_ARATH (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 424.9 bits (1091), Expect = 8.2e-118
Identity = 189/253 (74.70%), Postives = 217/253 (85.77%), Query Frame = 1


Query: 72  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES 131
           V+ELL+C VC N M+PPI+QC NGHTLCS+CK+RV N CPTCR ELG+IRCLALEKVAES
Sbjct: 40  VYELLECPVCTNSMYPPIHQCHNGHTLCSTCKSRVHNRCPTCRQELGDIRCLALEKVAES 99

Query: 132 LELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHE 191
           LELPC Y NLGC+ +FPYYSKLKHE  C +RPY+CPYAG EC   GDI  LV HL+DDH+
Sbjct: 100 LELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFLVAHLRDDHK 159

Query: 192 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 251
           VDMH GC+F+HRYVKSNP+EVENATWMLTVF CFG+ FCLHFEAF+LG APVYMAFLRFM
Sbjct: 160 VDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAPVYMAFLRFM 219

Query: 252 GDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 311
           GD+ +A+ ++YSLEVGG+GRK  W+G PRS+RDSHRKVRDSHDGLIIQR +ALF SG + 
Sbjct: 220 GDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDK 279

Query: 312 MELKLKVTGRIWR 325
            ELKL+VTGRIW+
Sbjct: 280 KELKLRVTGRIWK 292

[Close]

BLAST of Csa4G285710.2 vs. Swiss-Prot
Match: SINA4_ARATH (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana GN=SINAT4 PE=2 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 2.2e-115
Identity = 187/253 (73.91%), Postives = 217/253 (85.77%), Query Frame = 1


Query: 72  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES 131
           V+ELL+C VC   M+PPI+QC NGHTLCS+CK RV N CPTCR ELG+IRCLALEKVAES
Sbjct: 58  VYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLALEKVAES 117

Query: 132 LELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHE 191
           LELPC + NLGC ++FPYYSKLKHE  C +RPY+CPYAG EC + GDIP LV HL+DDH+
Sbjct: 118 LELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVAHLRDDHK 177

Query: 192 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 251
           VDMH G +F+HRYVKSNP+EVENATWMLTVF+CFG+ FCLHFEAF+LG  PVYMAFLRFM
Sbjct: 178 VDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRFM 237

Query: 252 GDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 311
           GD+ +A+ +SYSLEVGG+GRKL W+G PRSIRDSHRKVRDS+DGLIIQR +ALF SG + 
Sbjct: 238 GDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMALFFSGGDR 297

Query: 312 MELKLKVTGRIWR 325
            ELKL+VTG+IW+
Sbjct: 298 KELKLRVTGKIWK 310

[Close]

BLAST of Csa4G285710.2 vs. TrEMBL
Match: A0A0A0KWV9_CUCSA (E3 ubiquitin-protein ligase OS=Cucumis sativus GN=Csa_4G285710 PE=3 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 6.8e-196
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 1


Query: 1   MSTIIEFREKGMYQQPKSREEHASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKA 60
           MSTIIEFREKGMYQQPKSREEHASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKA
Sbjct: 1   MSTIIEFREKGMYQQPKSREEHASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKA 60

Query: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120
           STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI
Sbjct: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120

Query: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180
           RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP
Sbjct: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180

Query: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240
           SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240

Query: 241 APVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300
           APVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR
Sbjct: 241 APVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300

Query: 301 KLALFCSGNEGMELKLKVTGRIWRVR 327
           KLALFCSGNEGMELKLKVTGRIWRVR
Sbjct: 301 KLALFCSGNEGMELKLKVTGRIWRVR 326

[Close]

BLAST of Csa4G285710.2 vs. TrEMBL
Match: D7SLE4_VITVI (E3 ubiquitin-protein ligase OS=Vitis vinifera GN=VIT_06s0004g01130 PE=3 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 4.4e-134
Identity = 226/299 (75.59%), Postives = 253/299 (84.62%), Query Frame = 1


Query: 30  CKEVTELRLTSIDSQPATSRPEFKGVTFRKASTGGFAR----SRNDVHELLDCTVCMNLM 89
           CKEV E R    D    T+  E +   FRKA+TG   +    S + VHELL+C VCM+LM
Sbjct: 8   CKEVCESRSAFADYDVTTANLELRSSPFRKATTGVGGKHGMPSTSGVHELLECPVCMDLM 67

Query: 90  HPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQNLGCID 149
           HPPI+QC NGHTLCS+CKARV NCCPTCR ELGNIRCLALEK+AESLELPC YQ  GC D
Sbjct: 68  HPPIHQCPNGHTLCSNCKARVHNCCPTCRFELGNIRCLALEKIAESLELPCRYQIFGCHD 127

Query: 150 MFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCSFSHRYV 209
           +FPYYSKLKHE+NC++RPY+CPYAG EC VTGDIP LVMHLKDDH+VDMHDGC+F+HRYV
Sbjct: 128 IFPYYSKLKHEQNCQFRPYSCPYAGAECSVTGDIPLLVMHLKDDHKVDMHDGCTFNHRYV 187

Query: 210 KSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 269
           KSNPQEVENATWMLTVFNCFGRQFCLHFEAF LG APVYMAFLRFMGD++EA++FSYSLE
Sbjct: 188 KSNPQEVENATWMLTVFNCFGRQFCLHFEAFHLGMAPVYMAFLRFMGDENEARKFSYSLE 247

Query: 270 VGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVTGRIWR 325
           VGG GRKLIWQG+PRSIRDSH+ VRDS DGL+IQRKLALF SG E  +LKLKV GRIW+
Sbjct: 248 VGGYGRKLIWQGVPRSIRDSHKMVRDSQDGLVIQRKLALFFSGGERQQLKLKVAGRIWK 306

[Close]

BLAST of Csa4G285710.2 vs. TrEMBL
Match: W9RIL7_9ROSA (E3 ubiquitin-protein ligase OS=Morus notabilis GN=L484_006976 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 5.7e-134
Identity = 230/307 (74.92%), Postives = 254/307 (82.74%), Query Frame = 1


Query: 24  SSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKASTGGFAR----SRNDVHELLDCT 83
           S    F  E+ E R+T ++   ATS  E +   F+K ++         S NDVHELL+C 
Sbjct: 2   SPGGGFSPEMMESRITFVELDAATSNLELRSSPFKKTASNLSGNHGISSTNDVHELLECP 61

Query: 84  VCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQ 143
           VCMNLM+PPI+QC +GHTLCSSCKARV NCCPTCR ELGNIRCLALEKVAESLELPC YQ
Sbjct: 62  VCMNLMYPPIHQCPSGHTLCSSCKARVHNCCPTCRQELGNIRCLALEKVAESLELPCRYQ 121

Query: 144 NLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCS 203
            LGC D+FPYY KLKHEKNCK+RP+NCPYAG EC VTGDIP LVMHLKD H+VDMHDG +
Sbjct: 122 ILGCHDIFPYYGKLKHEKNCKHRPFNCPYAGAECSVTGDIPFLVMHLKDGHKVDMHDGST 181

Query: 204 FSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQ 263
           F+HRYVKSNPQ++ENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+ +AKQ
Sbjct: 182 FNHRYVKSNPQDIENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDEDDAKQ 241

Query: 264 FSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG--MELKLK 323
           FSYSLEVGGNGRKLIWQG+PRSIRDSHRKVRDS DGLIIQR LALF SG  G   ELKLK
Sbjct: 242 FSYSLEVGGNGRKLIWQGVPRSIRDSHRKVRDSQDGLIIQRSLALFFSGGNGNKQELKLK 301

Query: 324 VTGRIWR 325
           V GRIWR
Sbjct: 302 VAGRIWR 308

[Close]

BLAST of Csa4G285710.2 vs. TrEMBL
Match: A0A0D2V8W6_GOSRA (E3 ubiquitin-protein ligase OS=Gossypium raimondii GN=B456_013G005700 PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 9.7e-134
Identity = 229/299 (76.59%), Postives = 254/299 (84.95%), Query Frame = 1


Query: 30  CKEVTELRLTSIDSQPATSRPEFKGVTFRKAST--GGF--ARSRNDVHELLDCTVCMNLM 89
           CK+V E R+T  +   ATS  E +G   RKA+T  GG   A S NDVHELL+C VC+NLM
Sbjct: 8   CKDVIESRITFAEYDMATSSAELRGSPLRKAATSLGGNLGATSTNDVHELLECPVCLNLM 67

Query: 90  HPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQNLGCID 149
           +PPIYQC NGHT+CS CKARV N CPTCR+ELGNIRCLALEKVAESLELPC YQ LGC D
Sbjct: 68  YPPIYQCPNGHTVCSICKARVHNSCPTCRNELGNIRCLALEKVAESLELPCRYQILGCHD 127

Query: 150 MFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCSFSHRYV 209
           +FPYYSKLKHEKNC+YRPY+CPYAG EC VTGDIP LV+HLK+DH+VDMHDGC+F+HRYV
Sbjct: 128 IFPYYSKLKHEKNCRYRPYHCPYAGAECSVTGDIPFLVVHLKNDHKVDMHDGCTFNHRYV 187

Query: 210 KSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 269
           KSNP EVENATWMLTVFNCFGRQFCLHFEAF +G APVYMAFLRFMGD+ EA+QFSYSLE
Sbjct: 188 KSNPHEVENATWMLTVFNCFGRQFCLHFEAFHIGMAPVYMAFLRFMGDEDEARQFSYSLE 247

Query: 270 VGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVTGRIWR 325
           VG  GRK+ WQG+PRSIRDSHRKVRDS DGLIIQR LALF SG +  ELKLKV GRIW+
Sbjct: 248 VGCYGRKVTWQGVPRSIRDSHRKVRDSQDGLIIQRNLALFFSGGDRQELKLKVAGRIWK 306

[Close]

BLAST of Csa4G285710.2 vs. TrEMBL
Match: M5WJ19_PRUPE (E3 ubiquitin-protein ligase OS=Prunus persica GN=PRUPE_ppa009039mg PE=3 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 1.1e-132
Identity = 226/305 (74.10%), Postives = 252/305 (82.62%), Query Frame = 1


Query: 24  SSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKASTGGFA----RSRNDVHELLDCT 83
           S    FC++V E R+   DS  ATS  + +   FRKA+ G        S ++VH+LL+C 
Sbjct: 2   SPGGRFCEDVAESRMAFADSDVATSTGDVRTSPFRKAAPGLLGIPGMPSNHNVHDLLECP 61

Query: 84  VCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQ 143
           VCMNLM PPI+QC NGHTLCSSCK RV NCCPTCR+ELGNIRCLALEKVAESL+LPC YQ
Sbjct: 62  VCMNLMCPPIHQCPNGHTLCSSCKVRVHNCCPTCRNELGNIRCLALEKVAESLDLPCRYQ 121

Query: 144 NLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCS 203
             GC D+FPYYSKLKHEK CKYRPYNCPYAG +C VTGDI  L+MHLK+DH+VDMHDGC+
Sbjct: 122 IYGCQDIFPYYSKLKHEKICKYRPYNCPYAGADCSVTGDIQLLMMHLKNDHKVDMHDGCT 181

Query: 204 FSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQ 263
           F+HRYVKSNPQEV+NATWMLT+FNCFGRQFCLHFEAF +GTAPVYMAFLRFMGDD EAKQ
Sbjct: 182 FNHRYVKSNPQEVDNATWMLTIFNCFGRQFCLHFEAFHIGTAPVYMAFLRFMGDDDEAKQ 241

Query: 264 FSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVT 323
           F+YSLEV G+GRKLIWQGIPRSIRDSHRKVRDS DGLII R LALF SG    ELKLKV 
Sbjct: 242 FTYSLEVSGSGRKLIWQGIPRSIRDSHRKVRDSQDGLIIPRNLALFFSGGNRQELKLKVA 301

Query: 324 GRIWR 325
           GRIW+
Sbjct: 302 GRIWK 306

[Close]

BLAST of Csa4G285710.2 vs. TAIR10
Match: AT3G58040.1 (AT3G58040.1 seven in absentia of Arabidopsis 2)

HSP 1 Score: 469.2 bits (1206), Expect = 2.1e-132
Identity = 219/301 (72.76%), Postives = 249/301 (82.72%), Query Frame = 1


Query: 28  SFCKEVTELRLTSIDSQPATSRPEFKGVTFRKASTGGFAR----SRNDVHELLDCTVCMN 87
           S  KEV E   T +D +  T++ E       K  + G  +    S N V+ELL+C VC N
Sbjct: 6   SALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTN 65

Query: 88  LMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQNLGC 147
           LM+PPI+QC NGHTLCS+CK RVQN CPTCR+ELGNIRCLALEKVAESLE+PC YQNLGC
Sbjct: 66  LMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGC 125

Query: 148 IDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCSFSHR 207
            D+FPYYSKLKHE++C++RPY CPYAG EC VTGDIP+LV+HLKDDH+VDMHDGC+F+HR
Sbjct: 126 HDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHR 185

Query: 208 YVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYS 267
           YVKSNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD++EAK+FSYS
Sbjct: 186 YVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYS 245

Query: 268 LEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVTGRIW 325
           LEVG +GRKL WQGIPRSIRDSHRKVRDS DGLII R LAL+ SG +  ELKL+VTGRIW
Sbjct: 246 LEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIW 305

[Close]

BLAST of Csa4G285710.2 vs. TAIR10
Match: AT2G41980.1 (AT2G41980.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 459.1 bits (1180), Expect = 2.2e-129
Identity = 216/298 (72.48%), Postives = 245/298 (82.21%), Query Frame = 1


Query: 28  SFCKEVTELRLTSIDSQPATSRPEFKGVTFRKASTG-GFARSRNDVHELLDCTVCMNLMH 87
           S  KE  E   T +D +   ++ E      +  S   G   S N V+ELL+C VC NLM+
Sbjct: 6   SALKEALESNSTGVDYEVKMAKVEANSKPTKSGSGSIGKFHSSNGVYELLECPVCTNLMY 65

Query: 88  PPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQNLGCIDM 147
           PPI+QC NGHTLCSSCK RVQN CPTCR+ELGNIRCLALEKVAESLE+PC YQNLGC D+
Sbjct: 66  PPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQDI 125

Query: 148 FPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCSFSHRYVK 207
           FPYYSKLKHE++C++R Y+CPYAG EC VTGDIP+LV HLKDDH+VDMHDGC+F+HRYVK
Sbjct: 126 FPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRYVK 185

Query: 208 SNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLEV 267
           SNP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD++EAK+FSYSLEV
Sbjct: 186 SNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEV 245

Query: 268 GGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVTGRIWR 325
           G + RKL WQGIPRSIRDSHRKVRDS DGLII R LAL+ SG++  ELKL+VTGRIW+
Sbjct: 246 GAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWK 303

[Close]

BLAST of Csa4G285710.2 vs. TAIR10
Match: AT3G61790.1 (AT3G61790.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 438.0 bits (1125), Expect = 5.3e-123
Identity = 197/265 (74.34%), Postives = 227/265 (85.66%), Query Frame = 1


Query: 60  ASTGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGN 119
           A+  G   +   VHELL+C VC N M+PPI+QC NGHTLCS+CKARV N CPTCR ELG+
Sbjct: 45  AAASGLLPTTTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGD 104

Query: 120 IRCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDI 179
           IRCLALEKVAESLELPC + +LGC ++FPYYSKLKHE  C +RPY+CPYAG EC VTGDI
Sbjct: 105 IRCLALEKVAESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDI 164

Query: 180 PSLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLG 239
           P LV HL+DDH+VDMH GC+F+HRYVKSNP+EVENATWMLTVF+CFG+ FCLHFEAF+LG
Sbjct: 165 PFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLG 224

Query: 240 TAPVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQ 299
            APVYMAFLRFMGD++EA+ ++YSLEVGG GRKLIW+G PRS+RDSHRKVRDSHDGLIIQ
Sbjct: 225 MAPVYMAFLRFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQ 284

Query: 300 RKLALFCSGNEGMELKLKVTGRIWR 325
           R +ALF SG +  ELKL+VTGRIW+
Sbjct: 285 RNMALFFSGGDRKELKLRVTGRIWK 309

[Close]

BLAST of Csa4G285710.2 vs. TAIR10
Match: AT4G27880.1 (AT4G27880.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 416.8 bits (1070), Expect = 1.3e-116
Identity = 187/253 (73.91%), Postives = 217/253 (85.77%), Query Frame = 1


Query: 72  VHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAES 131
           V+ELL+C VC   M+PPI+QC NGHTLCS+CK RV N CPTCR ELG+IRCLALEKVAES
Sbjct: 58  VYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLALEKVAES 117

Query: 132 LELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHE 191
           LELPC + NLGC ++FPYYSKLKHE  C +RPY+CPYAG EC + GDIP LV HL+DDH+
Sbjct: 118 LELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVAHLRDDHK 177

Query: 192 VDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFM 251
           VDMH G +F+HRYVKSNP+EVENATWMLTVF+CFG+ FCLHFEAF+LG  PVYMAFLRFM
Sbjct: 178 VDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRFM 237

Query: 252 GDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEG 311
           GD+ +A+ +SYSLEVGG+GRKL W+G PRSIRDSHRKVRDS+DGLIIQR +ALF SG + 
Sbjct: 238 GDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMALFFSGGDR 297

Query: 312 MELKLKVTGRIWR 325
            ELKL+VTG+IW+
Sbjct: 298 KELKLRVTGKIWK 310

[Close]

BLAST of Csa4G285710.2 vs. TAIR10
Match: AT5G53360.1 (AT5G53360.1 TRAF-like superfamily protein)

HSP 1 Score: 338.2 bits (866), Expect = 5.7e-93
Identity = 151/202 (74.75%), Postives = 173/202 (85.64%), Query Frame = 1


Query: 123 LALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSL 182
           +ALEKVAESLELPC Y NLGC+ +FPYYSKLKHE  C +RPY+CPYAG EC   GDI  L
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 183 VMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAP 242
           V HL+DDH+VDMH GC+F+HRYVKSNP+EVENATWMLTVF CFG+ FCLHFEAF+LG AP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 243 VYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKL 302
           VYMAFLRFMGD+ +A+ ++YSLEVGG+GRK  W+G PRS+RDSHRKVRDSHDGLIIQR +
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 303 ALFCSGNEGMELKLKVTGRIWR 325
           ALF SG +  ELKL+VTGRIW+
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWK 216

[Close]

BLAST of Csa4G285710.2 vs. NCBI nr
Match: gi|700198937|gb|KGN54095.1| (hypothetical protein Csa_4G285710 [Cucumis sativus])

HSP 1 Score: 691.0 bits (1782), Expect = 9.8e-196
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 1


Query: 1   MSTIIEFREKGMYQQPKSREEHASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKA 60
           MSTIIEFREKGMYQQPKSREEHASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKA
Sbjct: 1   MSTIIEFREKGMYQQPKSREEHASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKA 60

Query: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120
           STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI
Sbjct: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120

Query: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180
           RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP
Sbjct: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180

Query: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240
           SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240

Query: 241 APVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300
           APVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR
Sbjct: 241 APVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300

Query: 301 KLALFCSGNEGMELKLKVTGRIWRVR 327
           KLALFCSGNEGMELKLKVTGRIWRVR
Sbjct: 301 KLALFCSGNEGMELKLKVTGRIWRVR 326

[Close]

BLAST of Csa4G285710.2 vs. NCBI nr
Match: gi|659097725|ref|XP_008449780.1| (PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Cucumis melo])

HSP 1 Score: 684.9 bits (1766), Expect = 7.0e-194
Identity = 323/326 (99.08%), Postives = 324/326 (99.39%), Query Frame = 1


Query: 1   MSTIIEFREKGMYQQPKSREEHASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKA 60
           MSTIIEFREKGMYQQ KSREEHASSAASFCKEVTEL LTSIDSQPATSRPEFKGVTFRKA
Sbjct: 1   MSTIIEFREKGMYQQQKSREEHASSAASFCKEVTELHLTSIDSQPATSRPEFKGVTFRKA 60

Query: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120
           STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI
Sbjct: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNI 120

Query: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180
           RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP
Sbjct: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIP 180

Query: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240
           SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 181 SLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240

Query: 241 APVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300
           APVYMAFLRFMGDD+EAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR
Sbjct: 241 APVYMAFLRFMGDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300

Query: 301 KLALFCSGNEGMELKLKVTGRIWRVR 327
           KLALFCSGNEGMELKLKVTGRIWRVR
Sbjct: 301 KLALFCSGNEGMELKLKVTGRIWRVR 326

[Close]

BLAST of Csa4G285710.2 vs. NCBI nr
Match: gi|728812525|gb|KHG00872.1| (E3 ubiquitin-protein ligase SINAT2 -like protein [Gossypium arboreum])

HSP 1 Score: 493.4 bits (1269), Expect = 3.0e-136
Identity = 233/299 (77.93%), Postives = 256/299 (85.62%), Query Frame = 1


Query: 30  CKEVTELRLTSIDSQPATSRPEFKGVTFRKAST--GGF--ARSRNDVHELLDCTVCMNLM 89
           CK+V E R+T  +   ATS  E +G   RKA+T  GG   A S NDVHELL+C VC+NLM
Sbjct: 8   CKDVIESRITFAEYDMATSSAELRGSPLRKAATSLGGNLGATSTNDVHELLECPVCLNLM 67

Query: 90  HPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQNLGCID 149
           +PPIYQC NGHT+CS CKARV N CPTCR+ELGNIRCLALEKVAESLELPC YQ LGC D
Sbjct: 68  YPPIYQCPNGHTVCSICKARVHNSCPTCRNELGNIRCLALEKVAESLELPCRYQILGCHD 127

Query: 150 MFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCSFSHRYV 209
           +FPYYSKLKHEKNC+YRPYNCPYAG EC VTGDIP LVMHLK+DH+VDMHDGC+F+HRYV
Sbjct: 128 IFPYYSKLKHEKNCRYRPYNCPYAGAECSVTGDIPFLVMHLKNDHKVDMHDGCTFNHRYV 187

Query: 210 KSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 269
           KSNP EVENATWMLTVFNCFGRQFCLHFEAF +G APVYMAFLRFMGD+ EA+QFSYSLE
Sbjct: 188 KSNPHEVENATWMLTVFNCFGRQFCLHFEAFHIGMAPVYMAFLRFMGDEDEARQFSYSLE 247

Query: 270 VGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVTGRIWR 325
           VGG GRK+IWQG+PRSIRDSHRKVRDS DGLIIQR LALF SG +  ELKLKV GRIW+
Sbjct: 248 VGGYGRKVIWQGVPRSIRDSHRKVRDSQDGLIIQRNLALFFSGGDRQELKLKVAGRIWK 306

[Close]

BLAST of Csa4G285710.2 vs. NCBI nr
Match: gi|590569860|ref|XP_007011187.1| (Seven in absentia of 2 isoform 1 [Theobroma cacao])

HSP 1 Score: 491.1 bits (1263), Expect = 1.5e-135
Identity = 231/299 (77.26%), Postives = 253/299 (84.62%), Query Frame = 1


Query: 30  CKEVTELRLTSIDSQPATSRPEFKGVTFRKASTGGF----ARSRNDVHELLDCTVCMNLM 89
           CK+V E R+   D   ATS  E +G   RKA+TG      A S NDVHELL+C VC+NLM
Sbjct: 8   CKDVIESRVAFADYDMATSSMELRGSPLRKAATGLGGNLGATSTNDVHELLECPVCVNLM 67

Query: 90  HPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQNLGCID 149
           +PPIYQC NGHT+CS CKARV N CPTCR+ELGNIRCLALEKVAESLELPC YQ LGC D
Sbjct: 68  YPPIYQCPNGHTVCSICKARVHNSCPTCRNELGNIRCLALEKVAESLELPCRYQTLGCHD 127

Query: 150 MFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCSFSHRYV 209
           +FPYYSKLKHEKNC+YRPYNCPYAG EC VTG+I  LVMHLK+DH+VDMHDGC+F+HRYV
Sbjct: 128 IFPYYSKLKHEKNCRYRPYNCPYAGAECSVTGNISFLVMHLKNDHKVDMHDGCTFNHRYV 187

Query: 210 KSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 269
           KSNP EVENATWMLTVFNCFGRQFCLHFEAF +G APVYMAFLRFMGD+ EA+QFSYSLE
Sbjct: 188 KSNPHEVENATWMLTVFNCFGRQFCLHFEAFHIGMAPVYMAFLRFMGDEDEARQFSYSLE 247

Query: 270 VGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVTGRIWR 325
           VGGNGRK IWQG+PRSIRDSHRKVRDS DGLIIQR LALF SG +  ELKLKV GRIW+
Sbjct: 248 VGGNGRKFIWQGVPRSIRDSHRKVRDSQDGLIIQRNLALFFSGGDRQELKLKVAGRIWK 306

[Close]

BLAST of Csa4G285710.2 vs. NCBI nr
Match: gi|823248750|ref|XP_012457030.1| (PREDICTED: E3 ubiquitin-protein ligase SINAT2-like [Gossypium raimondii])

HSP 1 Score: 490.3 bits (1261), Expect = 2.5e-135
Identity = 228/299 (76.25%), Postives = 255/299 (85.28%), Query Frame = 1


Query: 30  CKEVTELRLTSIDSQPATSRPEFKGVTFRKAST---GGF-ARSRNDVHELLDCTVCMNLM 89
           CKEV E R+T  D   ATS  + +G   RK +T   G   + S NDVHELL+C VC+NLM
Sbjct: 8   CKEVIESRVTFSDYDMATSSADLRGSPLRKTATALSGNLGSTSMNDVHELLECPVCVNLM 67

Query: 90  HPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAESLELPCVYQNLGCID 149
           +PPIYQC NGHT+CS+CK RV NCCPTCR+ELGNIRCLALEKVAESLELPC YQ LGC D
Sbjct: 68  YPPIYQCPNGHTVCSTCKTRVHNCCPTCRNELGNIRCLALEKVAESLELPCSYQILGCND 127

Query: 150 MFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDHEVDMHDGCSFSHRYV 209
           +FPYYSKLKHEKNC+YRPY+CPYAG EC VTGDIP LVMHLK+DH+VDMHDGC+F+HRYV
Sbjct: 128 IFPYYSKLKHEKNCRYRPYSCPYAGAECSVTGDIPFLVMHLKNDHKVDMHDGCTFNHRYV 187

Query: 210 KSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDSEAKQFSYSLE 269
           KSNP EVENATWMLTVFNCFGRQFCLHFEAF +G APVYMAFLRFMGD+ EA+QFSYSLE
Sbjct: 188 KSNPHEVENATWMLTVFNCFGRQFCLHFEAFHIGMAPVYMAFLRFMGDEDEARQFSYSLE 247

Query: 270 VGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNEGMELKLKVTGRIWR 325
           VGG+GRK +WQG+PRSIRDSHRKVRDS DGLIIQR LAL+ SG +  ELKLKV GRIW+
Sbjct: 248 VGGSGRKFMWQGVPRSIRDSHRKVRDSQDGLIIQRNLALYFSGGDRQELKLKVAGRIWK 306

The following BLAST results are available for this feature:

BLAST of Csa4G285710.2 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SINA2_ARATH	3.8e-131	72.76	E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana GN=SINAT2 PE=1 SV=1	[more]
SINA1_ARATH	3.9e-128	72.48	Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana GN=SINAT1 PE...	[more]
SINA3_ARATH	9.4e-122	74.34	E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana GN=SINAT3 PE=2 SV=1	[more]
SINA5_ARATH	8.2e-118	74.70	E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana GN=SINAT5 PE=1 SV=2	[more]
SINA4_ARATH	2.2e-115	73.91	E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana GN=SINAT4 PE=2 SV=1	[more]

BLAST of Csa4G285710.2 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWV9_CUCSA	6.8e-196	100.00	E3 ubiquitin-protein ligase OS=Cucumis sativus GN=Csa_4G285710 PE=3 SV=1	[more]
D7SLE4_VITVI	4.4e-134	75.59	E3 ubiquitin-protein ligase OS=Vitis vinifera GN=VIT_06s0004g01130 PE=3 SV=1	[more]
W9RIL7_9ROSA	5.7e-134	74.92	E3 ubiquitin-protein ligase OS=Morus notabilis GN=L484_006976 PE=3 SV=1	[more]
A0A0D2V8W6_GOSRA	9.7e-134	76.59	E3 ubiquitin-protein ligase OS=Gossypium raimondii GN=B456_013G005700 PE=3 SV=1	[more]
M5WJ19_PRUPE	1.1e-132	74.10	E3 ubiquitin-protein ligase OS=Prunus persica GN=PRUPE_ppa009039mg PE=3 SV=1	[more]

BLAST of Csa4G285710.2 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G58040.1	2.1e-132	72.76	seven in absentia of Arabidopsis 2	[more]
AT2G41980.1	2.2e-129	72.48	Protein with RING/U-box and TRAF-like domains	[more]
AT3G61790.1	5.3e-123	74.34	Protein with RING/U-box and TRAF-like domains	[more]
AT4G27880.1	1.3e-116	73.91	Protein with RING/U-box and TRAF-like domains	[more]
AT5G53360.1	5.7e-93	74.75	TRAF-like superfamily protein	[more]

BLAST of Csa4G285710.2 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700198937\|gb\|KGN54095.1\|	9.8e-196	100.00	hypothetical protein Csa_4G285710 [Cucumis sativus]	[more]
gi\|659097725\|ref\|XP_008449780.1\|	7.0e-194	99.08	PREDICTED: E3 ubiquitin-protein ligase SINAT2 [Cucumis melo]	[more]
gi\|728812525\|gb\|KHG00872.1\|	3.0e-136	77.93	E3 ubiquitin-protein ligase SINAT2 -like protein [Gossypium arboreum]	[more]
gi\|590569860\|ref\|XP_007011187.1\|	1.5e-135	77.26	Seven in absentia of 2 isoform 1 [Theobroma cacao]	[more]
gi\|823248750\|ref\|XP_012457030.1\|	2.5e-135	76.25	PREDICTED: E3 ubiquitin-protein ligase SINAT2-like [Gossypium raimondii]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR004162	SINA-like
IPR008974	TRAF-like
IPR013010	Znf_SIAH
IPR013083	Znf_RING/FYVE/PHD
IPR013323	SIAH-type domain
IPR018121	7-in-absentia-prot_TRAF-dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0004842	ubiquitin-protein transferase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **Biological Process**
Term	Definition
GO:0006511	ubiquitin-dependent protein catabolic process
GO:0007275	multicellular organism development
GO:0016567	protein ubiquitination

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007275	multicellular organism development
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0016874	ligase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Csa4G285710	Csa4G285710	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Csa4G285710.2	Csa4G285710.2-protein	polypeptide

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csa4G285710.2.utr3p1	Csa4G285710.2.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csa4G285710.2.cds3	Csa4G285710.2.cds3	CDS
Csa4G285710.2.cds2	Csa4G285710.2.cds2	CDS
Csa4G285710.2.cds1	Csa4G285710.2.cds1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csa4G285710.2.utr5p1	Csa4G285710.2.utr5p1	five_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 78..114 score: 9
IPR004162	E3 ubiquitin-protein ligase SIN-like	PANTHER	PTHR10315	SEVEN IN ABSENTIA HOMOLOG	coord: 68..324 score: 4.8E
IPR008974	TRAF-like	GENE3D	G3DSA:2.60.210.10		coord: 196..323 score: 1.5
IPR008974	TRAF-like	unknown	SSF49599	TRAF domain-like	coord: 131..322 score: 5.62
IPR013010	Zinc finger, SIAH-type	PROFILE	PS51081	ZF_SIAH	coord: 131..191 score: 13
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 66..134 score: 2.
IPR013323	SIAH-type domain	GENE3D	G3DSA:3.90.890.10		coord: 135..186 score: 4.4
IPR018121	Seven-in-absentia protein, TRAF-like domain	PFAM	PF03145	Sina	coord: 120..319 score: 9.1
None	No IPR available	PANTHER	PTHR10315:SF45	E3 UBIQUITIN-PROTEIN LIGASE	coord: 68..324 score: 4.8E
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 60..118 score: 6.0