Csa2G377880.1 (mRNA) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa2G377880.1
Type	mRNA
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Receptor protein kinase, putative; contains IPR013210 (Leucine-rich repeat-containing N-terminal, type 2)
Location	Chr2 : 19025970 .. 19026662 (+)
Sequence length	693

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAAACCTCCCCTCTTCTTCTTCTTCCTCTTCTTCCTCCTCTTCAACCTCCCACCGTCCACCTCCGCCGTCCCTACCTCCACCGAAACCCTCCTCCGATTCAAATCCTCTCTCACCAACACCCTCGCATTATCCAACTGGAACTCCTCCGTTCCTCTTTGTTCCGGCGACCGCCGCTTCTGGACCGGCCTAATCTGCAAAAACGACCAACTGTACGGCATCCGCCTCGAGAACATGAGTCTTGGCGGGACCGTCGACACCGCCGCTCTAGCCGGCCTCCCGACGTTGAGGACTCTGAGTGTGATGAACAATAGATTTGAAGGCCCAATGCCCGATGTGAAGCGAATAGGAGCTCTTAGGGCTTTGTATCTTTCGAATAATAACTTCTCGGGTTCGATTTCGGGAGATGCGTTTGAAGGAATGGGGAATTTGAAGAGATTGTATTTGAGTGGGAATGGATTTTCCGGGGAGATTCCGGGGTCGTTGGTGGAGTTGAAAGCGGTGGTGGAGTTGGGATTGGAGGATAATATGTTTGAAGGAAGAATACCGGATTTGGGTGAAAGGGTTTGGAAATATTTGAATTTTTCGGGGAATCGTTTGGATGGGCCGATTCCTTATGGATTGAGTAAAGACAGTAATTTCACTTCCTACCTTGGTAAGTTCACTCTTCTAAATCCTTTGCTTAACTAA

mRNA sequence

ATGGCCAAACCTCCCCTCTTCTTCTTCTTCCTCTTCTTCCTCCTCTTCAACCTCCCACCGTCCACCTCCGCCGTCCCTACCTCCACCGAAACCCTCCTCCGATTCAAATCCTCTCTCACCAACACCCTCGCATTATCCAACTGGAACTCCTCCGTTCCTCTTTGTTCCGGCGACCGCCGCTTCTGGACCGGCCTAATCTGCAAAAACGACCAACTGTACGGCATCCGCCTCGAGAACATGAGTCTTGGCGGGACCGTCGACACCGCCGCTCTAGCCGGCCTCCCGACGTTGAGGACTCTGAGTGTGATGAACAATAGATTTGAAGGCCCAATGCCCGATGTGAAGCGAATAGGAGCTCTTAGGGCTTTGTATCTTTCGAATAATAACTTCTCGGGTTCGATTTCGGGAGATGCGTTTGAAGGAATGGGGAATTTGAAGAGATTGTATTTGAGTGGGAATGGATTTTCCGGGGAGATTCCGGGGTCGTTGGTGGAGTTGAAAGCGGTGGTGGAGTTGGGATTGGAGGATAATATGTTTGAAGGAAGAATACCGGATTTGGGTGAAAGGGTTTGGAAATATTTGAATTTTTCGGGGAATCGTTTGGATGGGCCGATTCCTTATGGATTGAGTAAAGACAGTAATTTCACTTCCTACCTTGGTAAGTTCACTCTTCTAAATCCTTTGCTTAACTAA

Coding sequence (CDS)

ATGGCCAAACCTCCCCTCTTCTTCTTCTTCCTCTTCTTCCTCCTCTTCAACCTCCCACCGTCCACCTCCGCCGTCCCTACCTCCACCGAAACCCTCCTCCGATTCAAATCCTCTCTCACCAACACCCTCGCATTATCCAACTGGAACTCCTCCGTTCCTCTTTGTTCCGGCGACCGCCGCTTCTGGACCGGCCTAATCTGCAAAAACGACCAACTGTACGGCATCCGCCTCGAGAACATGAGTCTTGGCGGGACCGTCGACACCGCCGCTCTAGCCGGCCTCCCGACGTTGAGGACTCTGAGTGTGATGAACAATAGATTTGAAGGCCCAATGCCCGATGTGAAGCGAATAGGAGCTCTTAGGGCTTTGTATCTTTCGAATAATAACTTCTCGGGTTCGATTTCGGGAGATGCGTTTGAAGGAATGGGGAATTTGAAGAGATTGTATTTGAGTGGGAATGGATTTTCCGGGGAGATTCCGGGGTCGTTGGTGGAGTTGAAAGCGGTGGTGGAGTTGGGATTGGAGGATAATATGTTTGAAGGAAGAATACCGGATTTGGGTGAAAGGGTTTGGAAATATTTGAATTTTTCGGGGAATCGTTTGGATGGGCCGATTCCTTATGGATTGAGTAAAGACAGTAATTTCACTTCCTACCTTGGTAAGTTCACTCTTCTAAATCCTTTGCTTAACTAA

Protein sequence

MAKPPLFFFFLFFLLFNLPPSTSAVPTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRRFWTGLICKNDQLYGIRLENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGALRALYLSNNNFSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFEGRIPDLGERVWKYLNFSGNRLDGPIPYGLSKDSNFTSYLGKFTLLNPLLN*

Homology

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BLAST of Csa2G377880.1 vs. Swiss-Prot
Match: PRK1_ARATH (Pollen receptor-like kinase 1 OS=Arabidopsis thaliana GN=PRK1 PE=1 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 3.6e-43
Identity = 88/186 (47.31%), Postives = 124/186 (66.67%), Query Frame = 1


Query: 27  TSTETLLRFKSSLT--NTLALSNWNSSVPLCSGDRRFWTGLICKNDQLYGIRLENMSLGG 86
           + +E +L+FK SL      AL++WN+  P C+     W+G++C    ++ +++EN+ L G
Sbjct: 33  SDSEAILKFKESLVVGQENALASWNAKSPPCT-----WSGVLCNGGSVWRLQMENLELSG 92

Query: 87  TVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGALRALYLSNNNFSGSISGDAFEGMGN 146
           ++D  AL+GL +LRTLS MNN+FEGP PD K++ AL++LYLSNN F G I GDAFEGMG 
Sbjct: 93  SIDIEALSGLTSLRTLSFMNNKFEGPFPDFKKLAALKSLYLSNNQFGGDIPGDAFEGMGW 152

Query: 147 LKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFEGRIPDLGERVWKYLNFSGNRLDGP 206
           LK+++L+ N F+G+IP S+ +L  ++EL L+ N F G IP+  E     LN S N L GP
Sbjct: 153 LKKVHLAQNKFTGQIPSSVAKLPKLLELRLDGNQFTGEIPEF-EHQLHLLNLSNNALTGP 212

Query: 207 IPYGLS 211
           IP  LS
Sbjct: 213 IPESLS 212

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BLAST of Csa2G377880.1 vs. Swiss-Prot
Match: PRK4_ARATH (Pollen receptor-like kinase 4 OS=Arabidopsis thaliana GN=PRK4 PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 3.0e-42
Identity = 91/214 (42.52%), Postives = 124/214 (57.94%), Query Frame = 1


Query: 20  PSTSAVP---TSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRRFWTGLICKNDQLYGIR 79
           P    +P   +  + LLRFK +L N   +S+W+ S+  C  +   W G++C    ++G++
Sbjct: 41  PQADVLPLPASDADCLLRFKDTLVNASFISSWDPSISPCKRNSENWFGVLCVTGNVWGLQ 100

Query: 80  LENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGALRALYLSNNNFSGSISG 139
           LE M L G +D   LA +  LRTLS MNN+F G MP VK  GAL++LYLSNN F+G I  
Sbjct: 101 LEGMGLTGKLDLEPLAAIKNLRTLSFMNNKFNGSMPSVKNFGALKSLYLSNNRFTGEIPA 160

Query: 140 DAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFEGRIPDLGERVWKYLNF 199
           DAF+GM +LK+L L+ N F G IP SL  L  ++EL L  N F G IP   ++  K  +F
Sbjct: 161 DAFDGMHHLKKLLLANNAFRGSIPSSLAYLPMLLELRLNGNQFHGEIPYFKQKDLKLASF 220

Query: 200 SGNRLDGPIPYGLSKDSNFTSYLGKFTLLNPLLN 231
             N L+GPIP  LS + +  S+ G   L  P L+
Sbjct: 221 ENNDLEGPIPESLS-NMDPVSFSGNKNLCGPPLS 253

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BLAST of Csa2G377880.1 vs. Swiss-Prot
Match: PRK2_ARATH (Pollen receptor-like kinase 2 OS=Arabidopsis thaliana GN=PRK2 PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 3.1e-39
Identity = 88/186 (47.31%), Postives = 117/186 (62.90%), Query Frame = 1


Query: 27  TSTETLLRFKSSLT--NTLALSNWNSSVPLCSGDRRFWTGLICKNDQLYGIRLENMSLGG 86
           + TETLL+FK+SL      AL +WN   P C      WTG++C    ++G+RLEN+ L G
Sbjct: 23  SETETLLKFKNSLVIGRANALESWNRRNPPCK-----WTGVLCDRGFVWGLRLENLELSG 82

Query: 87  TVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGALRALYLSNNNFSGSISGDAFEGMGN 146
           ++D  AL GL +LR+LS +NN+F+GP P+ K++ AL++LYLSNN F   I  DAF+GMG 
Sbjct: 83  SIDIEALMGLNSLRSLSFINNKFKGPFPEFKKLVALKSLYLSNNQFDLEIPKDAFDGMGW 142

Query: 147 LKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFEGRIPDLGERVWKYLNFSGNRLDGP 206
           LK+L+L  N F GEIP SLV+   ++EL L+ N F G+IP+        LN S N L G 
Sbjct: 143 LKKLHLEQNNFIGEIPTSLVKSPKLIELRLDGNRFTGQIPEFRHHP-NMLNLSNNALAGQ 202

Query: 207 IPYGLS 211
           IP   S
Sbjct: 203 IPNSFS 202

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BLAST of Csa2G377880.1 vs. Swiss-Prot
Match: Y4312_ARATH (Probable LRR receptor-like serine/threonine-protein kinase At4g31250 OS=Arabidopsis thaliana GN=At4g31250 PE=2 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 5.4e-39
Identity = 96/231 (41.56%), Postives = 131/231 (56.71%), Query Frame = 1


Query: 4   PPLFFFFLFFLLFNLPPSTSAVPTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRRF-- 63
           P ++   L  LLF  P          + LL+FKSSL N  +L  W+S  P CSGD+    
Sbjct: 8   PIVYSLLLIVLLFVSPIYGDG---DADALLKFKSSLVNASSLGGWDSGEPPCSGDKGSDS 67

Query: 64  -WTGLICKNDQLYGIRLENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMP-DVKRIGA 123
            W G++C N  ++ +RLENMSL G +D  AL  +  L+++S M N FEG +P  +  + +
Sbjct: 68  KWKGVMCSNGSVFALRLENMSLSGELDVQALGSIRGLKSISFMRNHFEGKIPRGIDGLVS 127

Query: 124 LRALYLSNNNFSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMF 183
           L  LYL++N F+G I GD F GM  L +++L GN FSGEIP SL +L  + EL LEDNMF
Sbjct: 128 LAHLYLAHNQFTGEIDGDLFSGMKALLKVHLEGNRFSGEIPESLGKLPKLTELNLEDNMF 187

Query: 184 EGRIPDLGERVWKYLNFSGNRLDGPIPYGLSKDSNFTSYLGKFTLLN-PLL 230
            G+IP   ++    +N + N+L+G IP  L    N T + G   L   PLL
Sbjct: 188 TGKIPAFKQKNLVTVNVANNQLEGRIPLTLGL-MNITFFSGNKGLCGAPLL 234

[Close]

BLAST of Csa2G377880.1 vs. Swiss-Prot
Match: PRK5_ARATH (Pollen receptor-like kinase 5 OS=Arabidopsis thaliana GN=PRK5 PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 5.4e-39
Identity = 87/208 (41.83%), Postives = 117/208 (56.25%), Query Frame = 1


Query: 5   PLFFFFLFFLLFNLPPSTSAV---PTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRRF 64
           P   F + F+    P + S V    +  + LLRFK +L N     +W+     C G+   
Sbjct: 21  PSCIFIIIFISVLCPVAMSQVVVPDSDADCLLRFKDTLANGSEFRSWDPLSSPCQGNTAN 80

Query: 65  WTGLICKNDQLYGIRLENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGALR 124
           W G++C N  ++G++LE M L G ++   L  +  LRT+S MNN F GPMP VKR  +L+
Sbjct: 81  WFGVLCSN-YVWGLQLEGMGLTGKLNLDPLVPMKNLRTISFMNNNFNGPMPQVKRFTSLK 140

Query: 125 ALYLSNNNFSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFEG 184
           +LYLSNN FSG I  DAF GM  LK++ L+ N F G IP SL  L  ++EL L  N F+G
Sbjct: 141 SLYLSNNRFSGEIPADAFLGMPLLKKILLANNAFRGTIPSSLASLPMLLELRLNGNQFQG 200

Query: 185 RIPDLGERVWKYLNFSGNRLDGPIPYGL 210
           +IP   ++  K  +F  N LDGPIP  L
Sbjct: 201 QIPSFQQKDLKLASFENNDLDGPIPESL 227

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BLAST of Csa2G377880.1 vs. TrEMBL
Match: A0A0A0LPV8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G377880 PE=4 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 2.5e-128
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 1


Query: 1   MAKPPLFFFFLFFLLFNLPPSTSAVPTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRR 60
           MAKPPLFFFFLFFLLFNLPPSTSAVPTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRR
Sbjct: 1   MAKPPLFFFFLFFLLFNLPPSTSAVPTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRR 60

Query: 61  FWTGLICKNDQLYGIRLENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGAL 120
           FWTGLICKNDQLYGIRLENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGAL
Sbjct: 61  FWTGLICKNDQLYGIRLENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGAL 120

Query: 121 RALYLSNNNFSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFE 180
           RALYLSNNNFSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFE
Sbjct: 121 RALYLSNNNFSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFE 180

Query: 181 GRIPDLGERVWKYLNFSGNRLDGPIPYGLSKDSNFTSYLGKFTLLNPLLN 231
           GRIPDLGERVWKYLNFSGNRLDGPIPYGLSKDSNFTSYLGKFTLLNPLLN
Sbjct: 181 GRIPDLGERVWKYLNFSGNRLDGPIPYGLSKDSNFTSYLGKFTLLNPLLN 230

[Close]

BLAST of Csa2G377880.1 vs. TrEMBL
Match: W9SHU2_9ROSA (Putative LRR receptor-like serine/threonine-protein kinase OS=Morus notabilis GN=L484_019865 PE=4 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 3.3e-51
Identity = 109/202 (53.96%), Postives = 138/202 (68.32%), Query Frame = 1


Query: 11  LFFLLFNLPPSTSAVPTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRRFWTGLICKND 70
           L  LL    P+ SA PT  E LL+FKSSL+N  ALS+WN+SVPLC+G++  W+GL C N 
Sbjct: 18  LIVLLLRSTPA-SAAPTDAELLLKFKSSLSNAAALSDWNASVPLCNGNKNNWSGLTCMNG 77

Query: 71  QLYGIRLENMSLGGTVDTAALAGLPT-LRTLSVMNNRFEGPMPDVKRIGALRALYLSNNN 130
            LYG+RLENM L G +D   L+ L + LR+LSVM N F G +P V R+G LRAL+LS++ 
Sbjct: 78  NLYGLRLENMGLMGVIDVETLSQLSSDLRSLSVMYNSFTGAIPPVGRLGGLRALFLSHSG 137

Query: 131 FSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFEGRIPDLGER 190
           FSG I  DAFEGM  LK++YL+ NGF G +P SLV L  +VEL +E N   G+IP   +R
Sbjct: 138 FSGKIPHDAFEGMKYLKKVYLARNGFGGRLPSSLVGLSKLVELDVEGNQLGGKIPGFEQR 197

Query: 191 VWKYLNFSGNRLDGPIPYGLSK 212
            W+ LNF+ NR  G IP  LSK
Sbjct: 198 DWRVLNFANNRFKGRIPASLSK 218

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BLAST of Csa2G377880.1 vs. TrEMBL
Match: A0A061G9L2_THECC (Leucine-rich repeat protein kinase family protein, putative OS=Theobroma cacao GN=TCM_027719 PE=4 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.0e-49
Identity = 99/185 (53.51%), Postives = 131/185 (70.81%), Query Frame = 1


Query: 27  TSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRRFWTGLICKNDQLYGIRLENMSLGGTV 86
           T  ETLL FK SL N  ALSNWN+S+  C  DR  W G++C N+ ++G++LENM L G V
Sbjct: 36  TDMETLLTFKDSLANPSALSNWNASISPCHKDRANWIGVLCLNNTIWGLQLENMGLAGLV 95

Query: 87  DTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGALRALYLSNNNFSGSISGDAFEGMGNLK 146
           +   LA LP LRT+S+MNN FEG MPD++++GAL+ALYLSNN F+G I  DAF+GM +LK
Sbjct: 96  NIEILAALPNLRTISLMNNNFEGTMPDIRKLGALKALYLSNNRFAGQIPNDAFKGMRSLK 155

Query: 147 RLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFEGRIPDLGERVWKYLNFSGNRLDGPIP 206
           +++L+ N F+G IP SL  L  +V L LE N F G+IPD   +  K +N + N+L+GPIP
Sbjct: 156 KVFLANNAFTGTIPLSLTTLPKLVILRLEGNQFVGQIPDFKHKSVKVVNLASNQLEGPIP 215

Query: 207 YGLSK 212
             LSK
Sbjct: 216 ASLSK 220

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BLAST of Csa2G377880.1 vs. TrEMBL
Match: M5XIN2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa016973mg PE=4 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.0e-49
Identity = 105/215 (48.84%), Postives = 141/215 (65.58%), Query Frame = 1


Query: 11  LFFLLFNLPPSTSAVPTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRRFWTGLIC-KN 70
           LFFL       +S+ P+  +TLL+FKS L+N  AL NWN+S   C+G    W GL C KN
Sbjct: 17  LFFLAQLCIFVSSSDPSEIQTLLKFKSFLSNDTALDNWNNSTNPCNGTANIWAGLYCDKN 76

Query: 71  DQLYGIRLENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGALRALYLSNNN 130
             +YG++L+NM L G +D   L+GL  LR++S+MNN FEGP+P + ++GAL  +YLSNN 
Sbjct: 77  GNVYGLKLQNMGLMGIIDIDTLSGLSALRSISIMNNNFEGPLPSLNKLGALIGVYLSNNQ 136

Query: 131 FSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFEGRIPDLGE- 190
           F+G +  +AFEGM +LK++YL GN FSG+IP SL  L  + ELGL+ N F G+IP+  + 
Sbjct: 137 FTGEMQDNAFEGMSSLKKIYLDGNEFSGKIPKSLAALSKLSELGLQGNQFSGKIPNFAQS 196

Query: 191 -RVWKYLNFSGNRLDGPIPYGLS--KDSNFTSYLG 221
              WKY N S NR +G IP  LS  + S FT  LG
Sbjct: 197 PNGWKYFNISNNRFEGRIPASLSNLEASAFTGNLG 231

[Close]

BLAST of Csa2G377880.1 vs. TrEMBL
Match: A0A0A0LGT6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G882950 PE=4 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 5.7e-48
Identity = 106/213 (49.77%), Postives = 138/213 (64.79%), Query Frame = 1


Query: 1   MAKPPLFFFFLFFLLFNLPPSTSAVPTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRR 60
           MA        + FL++    S+S  P + E L++FKSSL++  AL NWN S+ +C  D +
Sbjct: 1   MAHKVDLLLLILFLVWCCNTSSSTSPEA-EILIKFKSSLSHNPALDNWNVSINICDDDAK 60

Query: 61  ---FWTGLICKNDQLYGIRLENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRI 120
              FWTG+ CK+  L+G+RLENMSL G +D   L  L +LR+LS MNN F G MP V+++
Sbjct: 61  TKGFWTGVTCKDGALFGLRLENMSLSGFIDIDVLMNLTSLRSLSFMNNSFHGSMPPVRKL 120

Query: 121 GALRALYLSNNNFSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDN 180
           GALRALYL+ N FSG+I  DAF+ M +LK + L  N F G IP SL  L A+VEL LE N
Sbjct: 121 GALRALYLAYNKFSGTIPDDAFQDMRSLKTVRLEENAFKGGIPSSLSSLPALVELSLEGN 180

Query: 181 MFEGRIPDLGERVWKYLNFSGNRLDGPIPYGLS 211
            FEGRIPD   R WK  + S N+L+G IP GL+
Sbjct: 181 RFEGRIPDFIPRDWKLFDLSNNQLEGSIPSGLA 212

[Close]

BLAST of Csa2G377880.1 vs. TAIR10
Match: AT5G35390.1 (AT5G35390.1 Leucine-rich repeat protein kinase family protein)

HSP 1 Score: 176.4 bits (446), Expect = 2.0e-44
Identity = 88/186 (47.31%), Postives = 124/186 (66.67%), Query Frame = 1


Query: 27  TSTETLLRFKSSLT--NTLALSNWNSSVPLCSGDRRFWTGLICKNDQLYGIRLENMSLGG 86
           + +E +L+FK SL      AL++WN+  P C+     W+G++C    ++ +++EN+ L G
Sbjct: 33  SDSEAILKFKESLVVGQENALASWNAKSPPCT-----WSGVLCNGGSVWRLQMENLELSG 92

Query: 87  TVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGALRALYLSNNNFSGSISGDAFEGMGN 146
           ++D  AL+GL +LRTLS MNN+FEGP PD K++ AL++LYLSNN F G I GDAFEGMG 
Sbjct: 93  SIDIEALSGLTSLRTLSFMNNKFEGPFPDFKKLAALKSLYLSNNQFGGDIPGDAFEGMGW 152

Query: 147 LKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFEGRIPDLGERVWKYLNFSGNRLDGP 206
           LK+++L+ N F+G+IP S+ +L  ++EL L+ N F G IP+  E     LN S N L GP
Sbjct: 153 LKKVHLAQNKFTGQIPSSVAKLPKLLELRLDGNQFTGEIPEF-EHQLHLLNLSNNALTGP 212

Query: 207 IPYGLS 211
           IP  LS
Sbjct: 213 IPESLS 212

[Close]

BLAST of Csa2G377880.1 vs. TAIR10
Match: AT3G20190.1 (AT3G20190.1 Leucine-rich repeat protein kinase family protein)

HSP 1 Score: 173.3 bits (438), Expect = 1.7e-43
Identity = 91/214 (42.52%), Postives = 124/214 (57.94%), Query Frame = 1


Query: 20  PSTSAVP---TSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRRFWTGLICKNDQLYGIR 79
           P    +P   +  + LLRFK +L N   +S+W+ S+  C  +   W G++C    ++G++
Sbjct: 41  PQADVLPLPASDADCLLRFKDTLVNASFISSWDPSISPCKRNSENWFGVLCVTGNVWGLQ 100

Query: 80  LENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGALRALYLSNNNFSGSISG 139
           LE M L G +D   LA +  LRTLS MNN+F G MP VK  GAL++LYLSNN F+G I  
Sbjct: 101 LEGMGLTGKLDLEPLAAIKNLRTLSFMNNKFNGSMPSVKNFGALKSLYLSNNRFTGEIPA 160

Query: 140 DAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFEGRIPDLGERVWKYLNF 199
           DAF+GM +LK+L L+ N F G IP SL  L  ++EL L  N F G IP   ++  K  +F
Sbjct: 161 DAFDGMHHLKKLLLANNAFRGSIPSSLAYLPMLLELRLNGNQFHGEIPYFKQKDLKLASF 220

Query: 200 SGNRLDGPIPYGLSKDSNFTSYLGKFTLLNPLLN 231
             N L+GPIP  LS + +  S+ G   L  P L+
Sbjct: 221 ENNDLEGPIPESLS-NMDPVSFSGNKNLCGPPLS 253

[Close]

BLAST of Csa2G377880.1 vs. TAIR10
Match: AT1G72460.1 (AT1G72460.1 Leucine-rich repeat protein kinase family protein)

HSP 1 Score: 164.1 bits (414), Expect = 1.0e-40
Identity = 85/186 (45.70%), Postives = 117/186 (62.90%), Query Frame = 1


Query: 27  TSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRRFWTGLICKNDQLYGIRLENMSLGGTV 86
           T +E+LL+FK SL NT +L +W      C   +R W GL+C  + ++G+++E M L G V
Sbjct: 23  TESESLLKFKKSLNNTKSLDSWTPESEPCGASQR-WIGLLCNKNSVFGLQIEQMGLSGKV 82

Query: 87  DTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGALRALYLSNNNFSGSISGDAFEGMGNLK 146
           D A L  LP+LRT+S+MNN F G +P+  R+ AL++LY+S N FSG+I  D FE M +LK
Sbjct: 83  DVAPLKDLPSLRTISIMNNSFSGDIPEFNRLTALKSLYISGNRFSGNIPSDYFETMVSLK 142

Query: 147 RLYLSGNGFSGEIPGSL-VELKAVVELGLEDNMFEGRIPDLGERVWKYLNFSGNRLDGPI 206
           + +LS N FSG IP SL   L  ++EL LE+N F G IP+  +     ++ S N+L G I
Sbjct: 143 KAWLSNNHFSGLIPISLATTLPNLIELRLENNQFIGSIPNFTQTTLAIVDLSNNQLTGEI 202

Query: 207 PYGLSK 212
           P GL K
Sbjct: 203 PPGLLK 207

[Close]

BLAST of Csa2G377880.1 vs. TAIR10
Match: AT2G07040.1 (AT2G07040.1 Leucine-rich repeat protein kinase family protein)

HSP 1 Score: 163.3 bits (412), Expect = 1.8e-40
Identity = 88/186 (47.31%), Postives = 117/186 (62.90%), Query Frame = 1


Query: 27  TSTETLLRFKSSLT--NTLALSNWNSSVPLCSGDRRFWTGLICKNDQLYGIRLENMSLGG 86
           + TETLL+FK+SL      AL +WN   P C      WTG++C    ++G+RLEN+ L G
Sbjct: 23  SETETLLKFKNSLVIGRANALESWNRRNPPCK-----WTGVLCDRGFVWGLRLENLELSG 82

Query: 87  TVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGALRALYLSNNNFSGSISGDAFEGMGN 146
           ++D  AL GL +LR+LS +NN+F+GP P+ K++ AL++LYLSNN F   I  DAF+GMG 
Sbjct: 83  SIDIEALMGLNSLRSLSFINNKFKGPFPEFKKLVALKSLYLSNNQFDLEIPKDAFDGMGW 142

Query: 147 LKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFEGRIPDLGERVWKYLNFSGNRLDGP 206
           LK+L+L  N F GEIP SLV+   ++EL L+ N F G+IP+        LN S N L G 
Sbjct: 143 LKKLHLEQNNFIGEIPTSLVKSPKLIELRLDGNRFTGQIPEFRHHP-NMLNLSNNALAGQ 202

Query: 207 IPYGLS 211
           IP   S
Sbjct: 203 IPNSFS 202

[Close]

BLAST of Csa2G377880.1 vs. TAIR10
Match: AT1G50610.1 (AT1G50610.1 Leucine-rich repeat protein kinase family protein)

HSP 1 Score: 162.5 bits (410), Expect = 3.0e-40
Identity = 87/208 (41.83%), Postives = 117/208 (56.25%), Query Frame = 1


Query: 5   PLFFFFLFFLLFNLPPSTSAV---PTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRRF 64
           P   F + F+    P + S V    +  + LLRFK +L N     +W+     C G+   
Sbjct: 21  PSCIFIIIFISVLCPVAMSQVVVPDSDADCLLRFKDTLANGSEFRSWDPLSSPCQGNTAN 80

Query: 65  WTGLICKNDQLYGIRLENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGALR 124
           W G++C N  ++G++LE M L G ++   L  +  LRT+S MNN F GPMP VKR  +L+
Sbjct: 81  WFGVLCSN-YVWGLQLEGMGLTGKLNLDPLVPMKNLRTISFMNNNFNGPMPQVKRFTSLK 140

Query: 125 ALYLSNNNFSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFEG 184
           +LYLSNN FSG I  DAF GM  LK++ L+ N F G IP SL  L  ++EL L  N F+G
Sbjct: 141 SLYLSNNRFSGEIPADAFLGMPLLKKILLANNAFRGTIPSSLASLPMLLELRLNGNQFQG 200

Query: 185 RIPDLGERVWKYLNFSGNRLDGPIPYGL 210
           +IP   ++  K  +F  N LDGPIP  L
Sbjct: 201 QIPSFQQKDLKLASFENNDLDGPIPESL 227

[Close]

BLAST of Csa2G377880.1 vs. NCBI nr
Match: gi|700207733|gb|KGN62852.1| (hypothetical protein Csa_2G377880 [Cucumis sativus])

HSP 1 Score: 466.1 bits (1198), Expect = 3.6e-128
Identity = 230/230 (100.00%), Postives = 230/230 (100.00%), Query Frame = 1


Query: 1   MAKPPLFFFFLFFLLFNLPPSTSAVPTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRR 60
           MAKPPLFFFFLFFLLFNLPPSTSAVPTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRR
Sbjct: 1   MAKPPLFFFFLFFLLFNLPPSTSAVPTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRR 60

Query: 61  FWTGLICKNDQLYGIRLENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGAL 120
           FWTGLICKNDQLYGIRLENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGAL
Sbjct: 61  FWTGLICKNDQLYGIRLENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGAL 120

Query: 121 RALYLSNNNFSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFE 180
           RALYLSNNNFSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFE
Sbjct: 121 RALYLSNNNFSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFE 180

Query: 181 GRIPDLGERVWKYLNFSGNRLDGPIPYGLSKDSNFTSYLGKFTLLNPLLN 231
           GRIPDLGERVWKYLNFSGNRLDGPIPYGLSKDSNFTSYLGKFTLLNPLLN
Sbjct: 181 GRIPDLGERVWKYLNFSGNRLDGPIPYGLSKDSNFTSYLGKFTLLNPLLN 230

[Close]

BLAST of Csa2G377880.1 vs. NCBI nr
Match: gi|778672163|ref|XP_004138883.2| (PREDICTED: probable LRR receptor-like serine/threonine-protein kinase At4g31250 [Cucumis sativus])

HSP 1 Score: 446.4 bits (1147), Expect = 3.0e-122
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 1


Query: 1   MAKPPLFFFFLFFLLFNLPPSTSAVPTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRR 60
           MAKPPLFFFFLFFLLFNLPPSTSAVPTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRR
Sbjct: 1   MAKPPLFFFFLFFLLFNLPPSTSAVPTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRR 60

Query: 61  FWTGLICKNDQLYGIRLENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGAL 120
           FWTGLICKNDQLYGIRLENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGAL
Sbjct: 61  FWTGLICKNDQLYGIRLENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGAL 120

Query: 121 RALYLSNNNFSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFE 180
           RALYLSNNNFSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFE
Sbjct: 121 RALYLSNNNFSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFE 180

Query: 181 GRIPDLGERVWKYLNFSGNRLDGPIPYGLSKDSNFTSYLG 221
           GRIPDLGERVWKYLNFSGNRLDGPIPYGLSKDSNFTSYLG
Sbjct: 181 GRIPDLGERVWKYLNFSGNRLDGPIPYGLSKDSNFTSYLG 220

[Close]

BLAST of Csa2G377880.1 vs. NCBI nr
Match: gi|659088595|ref|XP_008445066.1| (PREDICTED: probable LRR receptor-like serine/threonine-protein kinase At4g31250 [Cucumis melo])

HSP 1 Score: 411.0 bits (1055), Expect = 1.4e-111
Identity = 205/221 (92.76%), Postives = 208/221 (94.12%), Query Frame = 1


Query: 1   MAKPPLFFFFLFF-LLFNLPPSTSAVPTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDR 60
           M KPPLFFFF FF LLFNLPP  SAV T TETLLRFKSSLTNTLALSNWNSS PLCSGDR
Sbjct: 1   MPKPPLFFFFFFFFLLFNLPPPASAVFTPTETLLRFKSSLTNTLALSNWNSSAPLCSGDR 60

Query: 61  RFWTGLICKNDQLYGIRLENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGA 120
           RFWTGLICKN QLYGIRLENMSLGGTVDTAALAGLPTLRTLS+MNN FEGPMPDVKRIGA
Sbjct: 61  RFWTGLICKNGQLYGIRLENMSLGGTVDTAALAGLPTLRTLSLMNNGFEGPMPDVKRIGA 120

Query: 121 LRALYLSNNNFSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMF 180
           LRALYLSNNNFSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDN+F
Sbjct: 121 LRALYLSNNNFSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNLF 180

Query: 181 EGRIPDLGERVWKYLNFSGNRLDGPIPYGLSKDSNFTSYLG 221
           EGRIPD  ERVWKYLNFSGNRLDGPIPYGL K+SNFTSYLG
Sbjct: 181 EGRIPDFDERVWKYLNFSGNRLDGPIPYGLRKNSNFTSYLG 221

[Close]

BLAST of Csa2G377880.1 vs. NCBI nr
Match: gi|703158960|ref|XP_010112127.1| (putative LRR receptor-like serine/threonine-protein kinase [Morus notabilis])

HSP 1 Score: 209.9 bits (533), Expect = 4.7e-51
Identity = 109/202 (53.96%), Postives = 138/202 (68.32%), Query Frame = 1


Query: 11  LFFLLFNLPPSTSAVPTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRRFWTGLICKND 70
           L  LL    P+ SA PT  E LL+FKSSL+N  ALS+WN+SVPLC+G++  W+GL C N 
Sbjct: 18  LIVLLLRSTPA-SAAPTDAELLLKFKSSLSNAAALSDWNASVPLCNGNKNNWSGLTCMNG 77

Query: 71  QLYGIRLENMSLGGTVDTAALAGLPT-LRTLSVMNNRFEGPMPDVKRIGALRALYLSNNN 130
            LYG+RLENM L G +D   L+ L + LR+LSVM N F G +P V R+G LRAL+LS++ 
Sbjct: 78  NLYGLRLENMGLMGVIDVETLSQLSSDLRSLSVMYNSFTGAIPPVGRLGGLRALFLSHSG 137

Query: 131 FSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFEGRIPDLGER 190
           FSG I  DAFEGM  LK++YL+ NGF G +P SLV L  +VEL +E N   G+IP   +R
Sbjct: 138 FSGKIPHDAFEGMKYLKKVYLARNGFGGRLPSSLVGLSKLVELDVEGNQLGGKIPGFEQR 197

Query: 191 VWKYLNFSGNRLDGPIPYGLSK 212
            W+ LNF+ NR  G IP  LSK
Sbjct: 198 DWRVLNFANNRFKGRIPASLSK 218

[Close]

BLAST of Csa2G377880.1 vs. NCBI nr
Match: gi|596225408|ref|XP_007224182.1| (hypothetical protein PRUPE_ppa016973mg [Prunus persica])

HSP 1 Score: 204.9 bits (520), Expect = 1.5e-49
Identity = 105/215 (48.84%), Postives = 141/215 (65.58%), Query Frame = 1


Query: 11  LFFLLFNLPPSTSAVPTSTETLLRFKSSLTNTLALSNWNSSVPLCSGDRRFWTGLIC-KN 70
           LFFL       +S+ P+  +TLL+FKS L+N  AL NWN+S   C+G    W GL C KN
Sbjct: 17  LFFLAQLCIFVSSSDPSEIQTLLKFKSFLSNDTALDNWNNSTNPCNGTANIWAGLYCDKN 76

Query: 71  DQLYGIRLENMSLGGTVDTAALAGLPTLRTLSVMNNRFEGPMPDVKRIGALRALYLSNNN 130
             +YG++L+NM L G +D   L+GL  LR++S+MNN FEGP+P + ++GAL  +YLSNN 
Sbjct: 77  GNVYGLKLQNMGLMGIIDIDTLSGLSALRSISIMNNNFEGPLPSLNKLGALIGVYLSNNQ 136

Query: 131 FSGSISGDAFEGMGNLKRLYLSGNGFSGEIPGSLVELKAVVELGLEDNMFEGRIPDLGE- 190
           F+G +  +AFEGM +LK++YL GN FSG+IP SL  L  + ELGL+ N F G+IP+  + 
Sbjct: 137 FTGEMQDNAFEGMSSLKKIYLDGNEFSGKIPKSLAALSKLSELGLQGNQFSGKIPNFAQS 196

Query: 191 -RVWKYLNFSGNRLDGPIPYGLS--KDSNFTSYLG 221
              WKY N S NR +G IP  LS  + S FT  LG
Sbjct: 197 PNGWKYFNISNNRFEGRIPASLSNLEASAFTGNLG 231

The following BLAST results are available for this feature:

BLAST of Csa2G377880.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PRK1_ARATH	3.6e-43	47.31	Pollen receptor-like kinase 1 OS=Arabidopsis thaliana GN=PRK1 PE=1 SV=1	[more]
PRK4_ARATH	3.0e-42	42.52	Pollen receptor-like kinase 4 OS=Arabidopsis thaliana GN=PRK4 PE=1 SV=1	[more]
PRK2_ARATH	3.1e-39	47.31	Pollen receptor-like kinase 2 OS=Arabidopsis thaliana GN=PRK2 PE=1 SV=1	[more]
Y4312_ARATH	5.4e-39	41.56	Probable LRR receptor-like serine/threonine-protein kinase At4g31250 OS=Arabidop...	[more]
PRK5_ARATH	5.4e-39	41.83	Pollen receptor-like kinase 5 OS=Arabidopsis thaliana GN=PRK5 PE=1 SV=1	[more]

BLAST of Csa2G377880.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LPV8_CUCSA	2.5e-128	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G377880 PE=4 SV=1	[more]
W9SHU2_9ROSA	3.3e-51	53.96	Putative LRR receptor-like serine/threonine-protein kinase OS=Morus notabilis GN...	[more]
A0A061G9L2_THECC	1.0e-49	53.51	Leucine-rich repeat protein kinase family protein, putative OS=Theobroma cacao G...	[more]
M5XIN2_PRUPE	1.0e-49	48.84	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa016973mg PE=4 SV=1	[more]
A0A0A0LGT6_CUCSA	5.7e-48	49.77	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G882950 PE=4 SV=1	[more]

BLAST of Csa2G377880.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G35390.1	2.0e-44	47.31	Leucine-rich repeat protein kinase family protein	[more]
AT3G20190.1	1.7e-43	42.52	Leucine-rich repeat protein kinase family protein	[more]
AT1G72460.1	1.0e-40	45.70	Leucine-rich repeat protein kinase family protein	[more]
AT2G07040.1	1.8e-40	47.31	Leucine-rich repeat protein kinase family protein	[more]
AT1G50610.1	3.0e-40	41.83	Leucine-rich repeat protein kinase family protein	[more]

BLAST of Csa2G377880.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700207733\|gb\|KGN62852.1\|	3.6e-128	100.00	hypothetical protein Csa_2G377880 [Cucumis sativus]	[more]
gi\|778672163\|ref\|XP_004138883.2\|	3.0e-122	100.00	PREDICTED: probable LRR receptor-like serine/threonine-protein kinase At4g31250 ...	[more]
gi\|659088595\|ref\|XP_008445066.1\|	1.4e-111	92.76	PREDICTED: probable LRR receptor-like serine/threonine-protein kinase At4g31250 ...	[more]
gi\|703158960\|ref\|XP_010112127.1\|	4.7e-51	53.96	putative LRR receptor-like serine/threonine-protein kinase [Morus notabilis]	[more]
gi\|596225408\|ref\|XP_007224182.1\|	1.5e-49	48.84	hypothetical protein PRUPE_ppa016973mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001611	Leu-rich_rpt
IPR013210	LRR_N_plant-typ

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004672	protein kinase activity

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Csa2G377880	Csa2G377880	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Csa2G377880.1	Csa2G377880.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Csa2G377880.1.cds1	Csa2G377880.1.cds1	CDS

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001611	Leucine-rich repeat	PFAM	PF13855	LRR_8	coord: 120..178 score: 3.1
IPR013210	Leucine-rich repeat-containing N-terminal, plant-type	PFAM	PF08263	LRRNT_2	coord: 29..67 score: 2.
None	No IPR available	PANTHER	PTHR27008	FAMILY NOT NAMED	coord: 6..220 score: 4.8
None	No IPR available	PANTHER	PTHR27008:SF5	SUBFAMILY NOT NAMED	coord: 6..220 score: 4.8