Csa1G434690 (gene) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Csa1G434690
Type	gene
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	DNA polymerase epsilon subunit B, putative
Location	Chr1 : 15960589 .. 15962510 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGGAGAAAAAGTGAAGCAAAGTCTCAACCTAGAAGAATGAGCAGAAAGATAGGAGAATAGGTTGAGCCTCAGCGACTGAAACAGGTACGGCAACTTCCCCAACTGCTGAAACCCGGATACAGGAAGGAGAAGAACGGCTACGTGGACTGAGGCAGCTTCTAATGGCGCCGACTGATGGTTTGTGCGTCTTCTTATTGAAGTGTGAAATATGAGCTACTTGCTGACTCCCACTGGGTGGTAGGCTCTGGCCGGGTACCAAGAATTGAGTGAATATAGTGGGTTGGAAATCTTTGATCAAGTCAAGAGACCTTTCTCATTCCCGCAAGGACACAAGTACTTCTCCAACAAGAACTGAGAGCTACAGGAACAAGAACTAGCGGGACAAGAACTTAGTCAACACGAACAGCTACTGCAAGAGCGGCCTCTTGCCCTTGGACCACTCAAAATCAGGGAGCCACAAGGTTCGGATCCGTAGTAGTCTTTTCTTCTTCTTCCTATTCATTTGTTCTTTTTCTTCCTTTAACTGAAAACCTTTAACTGCGTTTTCTTGTTGGACTGATTACAATTGTATCTAGTAGAGCCAAAAAAGAATTTCGTCGTGAATCAACCCTCCATTTTCCAACCCCATCATCATTCCTTCACAATTATTGTGCAGAATTGCAGATTGTCTCACAGAAGAATGGACACATTAACTTTGAGGAAAAAGGTACAGAGGAAAGCTAAAATCAGAGGGTTGTACAGCATCAAACTGGAAGCTCTTGACGAGATTGTTTCCTTTGTCTCTCGTTCCCATGGCTTCGAAGATGAAGCTATAGAATTTGTTCTTGACAATCTCCATGAAGAATCTTGTACTGTTCCTTCTTCTTTCATACTACGATTATATCTTCTTTGCAATTCACCTCATTCCTTTTCATGGACAGTTTCTTTGTTTTGGCTATTGTGGCAGTGAAATCTCCAATATTAGACAAGGATGCGGTGCATCGAGTTATCAGCATTATGGTTGCAGCTGATAAAGTGGGAGAAGAAAGTCCCAATACCATTACCAGTACATCTGCTCTTTGCATCATCAACGCTTTTGATATCCCCAAGTTTCGTTATGATCCCATAAAAAAGATCTTTCTGTATGGTTTTCTCCCATTTATATTTATTTTTAATAGTTGTTAAACTAAGCATACCTGGTTAAGCATATACCTTGGGCCAAGAAGTCAGCAAAATCTCTCATTCCCATGTGTTGTTGAATACTTGAACTAAAAAAAAACTACTAGTTGGGTTTTTGGGATAATTAGACGCGGATAACTAATGCAGCTCATTGCAAAAAAGAGAGATATAATGGATTTAACTGAGAACTATCATCATCAACATTCCTTTTCTCTGCACTTGAAATCACATGTTCTTGAACCATGTACATATATATTTTTAGGCACACAGAAAACCTTCCAATTCATGGAGATGCTTCTGCTAAAGCTGCTTTATATAGAGATAGGTTTCTTTTGCTTTCCCAAAGGCTCTCTCGAGATCAGCATTTCTCCAAACCTGCTTTTGACATTGGCATGTCTCATTTTGGAAGTTGTGAGGTACTCCATTTCTCTTGACCTATGAATTGATCATGTTAGCCTTTACAATTGAACATGAGCATTTACACTAATTTTGTTTGTGATGTGTTTCCCAGATTTCTCCTATTCAATCTTTGGTGGGGCAAACAGGGAGAAAATGGGTCATGGGAGTGATCTCTCAAATGGAGGATGGCCACTTCTACTTGGAAGACCTTACCGCATCTGTGGAAATTAATTTATCTAGTGCTATATCCTTTACTGGCAACTTCTATGATGTGGAAAAATCTGAACTTGTTTGATATTTGAAGATGACATGCCCTGCAGTTTTCATTAATAGTTAGCACAAGATAACTACAGGTCTATTTACA

mRNA sequence

ATGGACACATTAACTTTGAGGAAAAAGGTACAGAGGAAAGCTAAAATCAGAGGGTTGTACAGCATCAAACTGGAAGCTCTTGACGAGATTGTTTCCTTTGTCTCTCGTTCCCATGGCTTCGAAGATGAAGCTATAGAATTTGTTCTTGACAATCTCCATGAAGAATCTTTGAAATCTCCAATATTAGACAAGGATGCGGTGCATCGAGTTATCAGCATTATGGTTGCAGCTGATAAAGTGGGAGAAGAAAGTCCCAATACCATTACCAGTACATCTGCTCTTTGCATCATCAACGCTTTTGATATCCCCAAGTTTCGTTATGATCCCATAAAAAAGATCTTTCTGCACACAGAAAACCTTCCAATTCATGGAGATGCTTCTGCTAAAGCTGCTTTATATAGAGATAGGTTTCTTTTGCTTTCCCAAAGGCTCTCTCGAGATCAGCATTTCTCCAAACCTGCTTTTGACATTGGCATGTCTCATTTTGGAAGTTGTGAGATTTCTCCTATTCAATCTTTGGTGGGGCAAACAGGGAGAAAATGGGTCATGGGAGTGATCTCTCAAATGGAGGATGGCCACTTCTACTTGGAAGACCTTACCGCATCTGTGGAAATTAATTTATCTAGTGCTATATCCTTTACTGGCAACTTCTATGATGTGGAAAAATCTGAACTTGTTTGA

Coding sequence (CDS)

ATGGACACATTAACTTTGAGGAAAAAGGTACAGAGGAAAGCTAAAATCAGAGGGTTGTACAGCATCAAACTGGAAGCTCTTGACGAGATTGTTTCCTTTGTCTCTCGTTCCCATGGCTTCGAAGATGAAGCTATAGAATTTGTTCTTGACAATCTCCATGAAGAATCTTTGAAATCTCCAATATTAGACAAGGATGCGGTGCATCGAGTTATCAGCATTATGGTTGCAGCTGATAAAGTGGGAGAAGAAAGTCCCAATACCATTACCAGTACATCTGCTCTTTGCATCATCAACGCTTTTGATATCCCCAAGTTTCGTTATGATCCCATAAAAAAGATCTTTCTGCACACAGAAAACCTTCCAATTCATGGAGATGCTTCTGCTAAAGCTGCTTTATATAGAGATAGGTTTCTTTTGCTTTCCCAAAGGCTCTCTCGAGATCAGCATTTCTCCAAACCTGCTTTTGACATTGGCATGTCTCATTTTGGAAGTTGTGAGATTTCTCCTATTCAATCTTTGGTGGGGCAAACAGGGAGAAAATGGGTCATGGGAGTGATCTCTCAAATGGAGGATGGCCACTTCTACTTGGAAGACCTTACCGCATCTGTGGAAATTAATTTATCTAGTGCTATATCCTTTACTGGCAACTTCTATGATGTGGAAAAATCTGAACTTGTTTGA

Protein sequence

MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSPILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENLPIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMGVISQMEDGHFYLEDLTASVEINLSSAISFTGNFYDVEKSELV*

Homology

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BLAST of Csa1G434690 vs. Swiss-Prot
Match: DPB2_ARATH (DNA polymerase epsilon subunit B OS=Arabidopsis thaliana GN=DPB2 PE=1 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 1.8e-55
Identity = 123/213 (57.75%), Postives = 156/213 (73.24%), Query Frame = 1


Query: 7   RKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFED-EAIEFVLDNLHEESLKSPILDKD 66
           RKK+Q+K K RG Y++K +ALDEI+ F  +    +D EAI+ +LDNL +E+ KS  +D +
Sbjct: 8   RKKIQKKFKNRG-YNLKFDALDEILVFADQFPDDDDGEAIDLLLDNL-QETHKSSTVDAE 67

Query: 67  AVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFL-HTENLPIHG 126
           +V  +I+ ++ A    EE     TS S+L II+AF +PKF YD +KK F  HT +LPIHG
Sbjct: 68  SVRGLINRLLGAHNAPEEPT---TSASSLAIIDAFLVPKFGYDSVKKKFNEHTSSLPIHG 127

Query: 127 DASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMG 186
           +ASAK ALYR+RF+LLSQR+SR +HFS+PAFD  MS F + EIS IQSL+ Q GRKWVMG
Sbjct: 128 EASAKTALYRERFMLLSQRVSRAEHFSRPAFDAEMSQFENNEISSIQSLISQRGRKWVMG 187

Query: 187 VISQMEDGHFYLEDLTASVEINLSSAISFTGNF 218
           VISQ+EDGHFYLEDL+ASVEI+LS A   TG F
Sbjct: 188 VISQLEDGHFYLEDLSASVEIDLSKAKITTGFF 215

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BLAST of Csa1G434690 vs. Swiss-Prot
Match: DPOE2_DICDI (DNA polymerase epsilon subunit 2 OS=Dictyostelium discoideum GN=pole2 PE=3 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 5.8e-22
Identity = 68/207 (32.85%), Postives = 118/207 (57.00%), Query Frame = 1


Query: 7   RKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSPILDK-D 66
           +K++ +   + G +SIK +A+  I++ + ++ G++   I  ++DNL +   KS  L   +
Sbjct: 6   KKQITKSFSLTG-FSIKADAMKCIINRI-KNEGYD---ITQLIDNLLKNLDKSQYLQTIE 65

Query: 67  AVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENLP--IH 126
              ++I  + ++D + +E         AL +I+AF+ P F +D   K F+ +EN    +H
Sbjct: 66  GCFKLIDTINSSDAIEKE---------ALKVIDAFNTPLFNFDSNSKQFIKSENFDRSLH 125

Query: 127 GDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVM 186
           G +++K+ LYR R+L + QR  R+ +FS P             I+P+ SL+G TGRK V+
Sbjct: 126 GSSNSKSDLYRKRYLKVLQRTERNDYFSTPVLASDKLKNEYQTITPLSSLLGNTGRKHVL 185

Query: 187 GVISQMEDGHFYLEDLTASVEINLSSA 211
           G ISQ+E+  FY+EDL  +V+I++S A
Sbjct: 186 GTISQIEEDQFYIEDLNTNVKIDISKA 198

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BLAST of Csa1G434690 vs. Swiss-Prot
Match: DPOE2_BOVIN (DNA polymerase epsilon subunit 2 OS=Bos taurus GN=POLE2 PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 8.2e-16
Identity = 66/220 (30.00%), Postives = 115/220 (52.27%), Query Frame = 1


Query: 6   LRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFE-DEAIEFVLDNLHEESLKSPILDK 65
           LR +     K+RGL  ++ EA+  +   +   +  E ++A+E ++D + ++ L S ++++
Sbjct: 6   LRSRALSAFKLRGLM-LRGEAVKYLTEALQSINEIELEDALEKIIDAVEKQPLSSNMIER 65

Query: 66  DAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHT--ENLP- 125
             V    ++   +  V E      T      II AFDIP+F Y+  +K FL     N P 
Sbjct: 66  SVVEA--AVQECSQSVDE------TIDHIFNIIGAFDIPRFVYNSERKKFLPLLMTNHPA 125

Query: 126 --IHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGS-CEISPIQSLVGQT- 185
             + G A  KA L+R+R+ +L QR  R + F+ P         GS  ++  I++L+G T 
Sbjct: 126 PNLFGTARDKAELFRERYTILHQRTHRHELFTPPVIGSHPDESGSKFQLKTIETLLGSTS 185

Query: 186 --GRKWVMGVISQMEDGHFYLEDLTASVEINLSSAISFTG 216
             G   V+G+I+Q+++G F+LED T +++++LS A   +G
Sbjct: 186 KIGDVIVLGMITQLKEGKFFLEDPTGTIQLDLSKAQFHSG 216

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BLAST of Csa1G434690 vs. Swiss-Prot
Match: DPOE2_CHICK (DNA polymerase epsilon subunit 2 OS=Gallus gallus GN=POLE2 PE=2 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.0e-14
Identity = 63/228 (27.63%), Postives = 119/228 (52.19%), Query Frame = 1


Query: 1   MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFE-DEAIEFVLDNLHEESLKS 60
           M+   LR+++    ++RGL  ++ EA+  +   +   +  E ++ I+ ++D + ++ L +
Sbjct: 1   MEPERLRRRLGSAFRVRGLL-LRAEAVKYLTETLESLNEEEFEDVIDKIIDAVEKQPLST 60

Query: 61  PILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIF--LHT 120
            +++   V    ++   +  + E   N         II AFDIP++ Y+P +K F  L  
Sbjct: 61  NMIELSTVEA--AVQECSQALDETIKNVFN------IIGAFDIPRYIYNPDRKRFVPLSM 120

Query: 121 ENLPI---HGDASAKAALYRDRFLLLSQRLSRDQHFSKPAF----DIGMSHFGSCEISPI 180
            NLP+    G A  KA L+R+R+ +L QR  R + F+  A     D   S F   ++  +
Sbjct: 121 TNLPVPNLFGTARDKAELFRERYSILQQRTHRHELFTPAAVVIHPDDNRSKF---QLKTV 180

Query: 181 QSLVGQTGRKW---VMGVISQMEDGHFYLEDLTASVEINLSSAISFTG 216
           ++L+G T +     V+G+I+Q+++G ++LED T  V+++LS A   +G
Sbjct: 181 ETLLGNTAKVREVIVLGMITQLKEGKYFLEDPTGVVQLDLSKAQFHSG 216

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BLAST of Csa1G434690 vs. Swiss-Prot
Match: DPOE2_MOUSE (DNA polymerase epsilon subunit 2 OS=Mus musculus GN=Pole2 PE=2 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 2.0e-14
Identity = 64/220 (29.09%), Postives = 108/220 (49.09%), Query Frame = 1


Query: 6   LRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFE-DEAIEFVLDNLHEESLKSPILDK 65
           LR +     K+RGL  ++ EA   +   +      E +E +  ++D + ++ L S ++++
Sbjct: 6   LRSRTVSAFKLRGLL-LRSEATKYLTEALQSVTELELEENLGKIIDAVEKQPLSSNMIER 65

Query: 66  DAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL---- 125
             V        A  +  +    TI       II AFDIP+F Y+  +K FL         
Sbjct: 66  SVVE------AAVQECSQSMDETIEHV--FNIIGAFDIPRFIYNSERKKFLPLSMTNHAA 125

Query: 126 -PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGS-CEISPIQSLVGQT- 185
             + G A  KA LY +R+ +L QR  R + F+ P     +   GS  ++  I++L+G T 
Sbjct: 126 PTLFGTARDKAELYLERYTILHQRTHRHELFTPPVIGSHLEESGSKFQLKTIETLLGSTS 185

Query: 186 --GRKWVMGVISQMEDGHFYLEDLTASVEINLSSAISFTG 216
             G   V+G+I+Q+++G F+LED T +V+++LS A   +G
Sbjct: 186 KVGDVIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSG 216

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BLAST of Csa1G434690 vs. TrEMBL
Match: A0A0A0LUV9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G434690 PE=4 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 5.9e-122
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 1


Query: 1   MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP 60
           MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP
Sbjct: 1   MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP 60

Query: 61  ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL 120
           ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL
Sbjct: 61  ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL 120

Query: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK 180
           PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK 180

Query: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAISFTGNFYDVEKSELV 227
           WVMGVISQMEDGHFYLEDLTASVEINLSSAISFTGNFYDVEKSELV
Sbjct: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAISFTGNFYDVEKSELV 226

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BLAST of Csa1G434690 vs. TrEMBL
Match: A5AMH8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_038180 PE=4 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 9.2e-75
Identity = 149/209 (71.29%), Postives = 178/209 (85.17%), Query Frame = 1


Query: 7   RKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSPILDKDA 66
           RKKVQRK KIRG Y++K+EALDEI+SF+S     EDEAI+ +LD L  ESLKS ILDK++
Sbjct: 6   RKKVQRKFKIRG-YTLKVEALDEILSFLSHFQDAEDEAIDLLLDELENESLKSSILDKES 65

Query: 67  VHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFL-HTENLPIHGD 126
           VHRV+S+++ A+   EE+P  I++ SAL I++AF + KF YDPIKKIF  +T  L IHGD
Sbjct: 66  VHRVVSLLLEAEAAVEETPGAISTRSALRIVDAFLMSKFCYDPIKKIFYENTGRLAIHGD 125

Query: 127 ASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMGV 186
           ASAK+ALYR+RFLLL QRLSRDQHFSKPAFD  MSHFGSCEISPIQSL+GQTG++WVMGV
Sbjct: 126 ASAKSALYRNRFLLLFQRLSRDQHFSKPAFDTEMSHFGSCEISPIQSLIGQTGKRWVMGV 185

Query: 187 ISQMEDGHFYLEDLTASVEINLSSAISFT 215
           ISQ+EDGHF+LEDLTASVEINLS+AISFT
Sbjct: 186 ISQLEDGHFFLEDLTASVEINLSNAISFT 213

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BLAST of Csa1G434690 vs. TrEMBL
Match: A0A161ZS40_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_023267 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 1.4e-70
Identity = 137/212 (64.62%), Postives = 176/212 (83.02%), Query Frame = 1


Query: 6   LRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSPILDKD 65
           +RK +Q+K K+RG Y++K+EAL+EI+ F+S+    EDEA++ +LD L  +SLKS ILDK+
Sbjct: 5   MRKLIQKKFKMRG-YTLKVEALNEILPFLSKFKDAEDEALDLLLDELQHQSLKSSILDKE 64

Query: 66  AVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFL-HTENLPIHG 125
           +V RV+S+++ A+   E++P + ++T AL II+AF +PK+RYDPIKKIFL HT  LPIHG
Sbjct: 65  SVSRVVSLLLEAEAAAEDTPASTSATGALRIIDAFIVPKYRYDPIKKIFLEHTGRLPIHG 124

Query: 126 DASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMG 185
           DASAKA LYRDRFLLL QRLSRD HFS+PAFD  +S FG+C+ISPIQSLVG+TG+ WVMG
Sbjct: 125 DASAKATLYRDRFLLLFQRLSRDPHFSRPAFDTDLSQFGNCQISPIQSLVGRTGKCWVMG 184

Query: 186 VISQMEDGHFYLEDLTASVEINLSSAISFTGN 217
           VISQ+EDGHFYLEDLTA+VEINLS+AIS   N
Sbjct: 185 VISQLEDGHFYLEDLTAAVEINLSNAISLPAN 215

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BLAST of Csa1G434690 vs. TrEMBL
Match: A0A059AW13_EUCGR (DNA polymerase epsilon subunit 2 OS=Eucalyptus grandis GN=EUGRSUZ_H00594 PE=3 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 3.1e-70
Identity = 141/215 (65.58%), Postives = 175/215 (81.40%), Query Frame = 1


Query: 6   LRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSPILDKD 65
           ++KKVQRK K+RG Y+++++ALD ++SF SR  G ED+A++ +LD L  ESLKS I+DKD
Sbjct: 5   MKKKVQRKFKMRG-YTLRVDALDAVLSFASRFAGAEDDALDLLLDQLDHESLKSSIIDKD 64

Query: 66  AVHRVISIMVAADKVGEESPNTITST--SALCIINAFDIPKFRYDPIKKIFL-HTENLPI 125
            V+RV+S+++ A+   EESP+       SAL +I+AF +PKFRYDP+KKIF  HT +LPI
Sbjct: 65  PVNRVVSLLLEAEAAVEESPDAACEGRGSALRVIDAFLVPKFRYDPVKKIFYEHTGSLPI 124

Query: 126 HGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWV 185
           HG+ASAKA LY+DRFLLL QRLSRDQHF+KPAF+   + FGSCEISPIQSLVGQTGR+WV
Sbjct: 125 HGEASAKAKLYKDRFLLLYQRLSRDQHFAKPAFETETTEFGSCEISPIQSLVGQTGRRWV 184

Query: 186 MGVISQMEDGHFYLEDLTASVEINLSSAISFTGNF 218
           MGVISQ+EDGHFYLEDLTASVEINLS A   TG F
Sbjct: 185 MGVISQLEDGHFYLEDLTASVEINLSDAKVTTGFF 218

[Close]

BLAST of Csa1G434690 vs. TrEMBL
Match: D7SIP4_VITVI (DNA polymerase epsilon subunit 2 OS=Vitis vinifera GN=VIT_17s0000g04830 PE=3 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 3.4e-69
Identity = 141/213 (66.20%), Postives = 172/213 (80.75%), Query Frame = 1


Query: 7   RKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSPILDKDA 66
           RKKVQRK KIRG Y++K+EALDEI+SF+S     EDEAI+ +LD L  ESLKS ILDK++
Sbjct: 6   RKKVQRKFKIRG-YTLKVEALDEILSFLSHFQDAEDEAIDLLLDELENESLKSSILDKES 65

Query: 67  VHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFL-HTENLPIHGD 126
           VHRV+S+++ A+   EE+P  I++ SAL I++AF + KF YDPIKKIF  +T  L IHGD
Sbjct: 66  VHRVVSLLLEAEAAVEETPGAISTRSALRIVDAFLMSKFCYDPIKKIFYENTGRLAIHGD 125

Query: 127 ASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMGV 186
           ASAK+ALYR+RFLLL QRLSRDQHFSKPAFD  MSHFGSCEISPIQSL+GQTG++WVMGV
Sbjct: 126 ASAKSALYRNRFLLLFQRLSRDQHFSKPAFDTEMSHFGSCEISPIQSLIGQTGKRWVMGV 185

Query: 187 ISQMEDGHFYLEDLTASVEINLSSAISFTGNFY 219
           ISQ+EDGHF+LEDLTAS+ + L      T  F+
Sbjct: 186 ISQLEDGHFFLEDLTASLAL-LHQQYKITTGFF 216

[Close]

BLAST of Csa1G434690 vs. TAIR10
Match: AT5G22110.1 (AT5G22110.1 DNA polymerase epsilon subunit B2)

HSP 1 Score: 217.2 bits (552), Expect = 1.0e-56
Identity = 123/213 (57.75%), Postives = 156/213 (73.24%), Query Frame = 1


Query: 7   RKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFED-EAIEFVLDNLHEESLKSPILDKD 66
           RKK+Q+K K RG Y++K +ALDEI+ F  +    +D EAI+ +LDNL +E+ KS  +D +
Sbjct: 8   RKKIQKKFKNRG-YNLKFDALDEILVFADQFPDDDDGEAIDLLLDNL-QETHKSSTVDAE 67

Query: 67  AVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFL-HTENLPIHG 126
           +V  +I+ ++ A    EE     TS S+L II+AF +PKF YD +KK F  HT +LPIHG
Sbjct: 68  SVRGLINRLLGAHNAPEEPT---TSASSLAIIDAFLVPKFGYDSVKKKFNEHTSSLPIHG 127

Query: 127 DASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMG 186
           +ASAK ALYR+RF+LLSQR+SR +HFS+PAFD  MS F + EIS IQSL+ Q GRKWVMG
Sbjct: 128 EASAKTALYRERFMLLSQRVSRAEHFSRPAFDAEMSQFENNEISSIQSLISQRGRKWVMG 187

Query: 187 VISQMEDGHFYLEDLTASVEINLSSAISFTGNF 218
           VISQ+EDGHFYLEDL+ASVEI+LS A   TG F
Sbjct: 188 VISQLEDGHFYLEDLSASVEIDLSKAKITTGFF 215

[Close]

BLAST of Csa1G434690 vs. NCBI nr
Match: gi|700210427|gb|KGN65523.1| (hypothetical protein Csa_1G434690 [Cucumis sativus])

HSP 1 Score: 444.9 bits (1143), Expect = 8.5e-122
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 1


Query: 1   MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP 60
           MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP
Sbjct: 1   MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP 60

Query: 61  ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL 120
           ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL
Sbjct: 61  ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL 120

Query: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK 180
           PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK 180

Query: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAISFTGNFYDVEKSELV 227
           WVMGVISQMEDGHFYLEDLTASVEINLSSAISFTGNFYDVEKSELV
Sbjct: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAISFTGNFYDVEKSELV 226

[Close]

BLAST of Csa1G434690 vs. NCBI nr
Match: gi|778660923|ref|XP_011657177.1| (PREDICTED: DNA polymerase epsilon subunit 2 [Cucumis sativus])

HSP 1 Score: 416.4 bits (1069), Expect = 3.2e-113
Identity = 213/217 (98.16%), Postives = 213/217 (98.16%), Query Frame = 1


Query: 1   MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP 60
           MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP
Sbjct: 1   MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP 60

Query: 61  ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL 120
           ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL
Sbjct: 61  ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFLHTENL 120

Query: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK 180
           PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK
Sbjct: 121 PIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRK 180

Query: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAISFTGNF 218
           WVMGVISQMEDGHFYLEDLTASVEINLSSA   TG F
Sbjct: 181 WVMGVISQMEDGHFYLEDLTASVEINLSSAKITTGLF 217

[Close]

BLAST of Csa1G434690 vs. NCBI nr
Match: gi|659066275|ref|XP_008463560.1| (PREDICTED: LOW QUALITY PROTEIN: DNA polymerase epsilon subunit 2 [Cucumis melo])

HSP 1 Score: 367.9 bits (943), Expect = 1.3e-98
Identity = 190/218 (87.16%), Postives = 201/218 (92.20%), Query Frame = 1


Query: 1   MDTLTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSP 60
           MD LTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSR HGFED+AIE VLD+LHEESLKSP
Sbjct: 1   MDALTLRKKVQRKAKIRGLYSIKLEALDEIVSFVSRFHGFEDDAIELVLDDLHEESLKSP 60

Query: 61  ILDKDAVHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIF-LHTEN 120
           IL+KD VHRVIS ++AA++  E SPNTITST+ALCII+AFDI KFRYDPIKKIF  HT +
Sbjct: 61  ILEKDGVHRVISKLLAAEEAEEASPNTITSTTALCIIDAFDISKFRYDPIKKIFHRHTGD 120

Query: 121 LPIHGDASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 180
           LPIHGDA AKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR
Sbjct: 121 LPIHGDAPAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGR 180

Query: 181 KWVMGVISQMEDGHFYLEDLTASVEINLSSAISFTGNF 218
           KWVMGVISQMEDGHFYLEDLTASVEINLS+A   TG F
Sbjct: 181 KWVMGVISQMEDGHFYLEDLTASVEINLSNAKITTGLF 218

[Close]

BLAST of Csa1G434690 vs. NCBI nr
Match: gi|147818232|emb|CAN73546.1| (hypothetical protein VITISV_038180 [Vitis vinifera])

HSP 1 Score: 288.1 bits (736), Expect = 1.3e-74
Identity = 149/209 (71.29%), Postives = 178/209 (85.17%), Query Frame = 1


Query: 7   RKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSPILDKDA 66
           RKKVQRK KIRG Y++K+EALDEI+SF+S     EDEAI+ +LD L  ESLKS ILDK++
Sbjct: 6   RKKVQRKFKIRG-YTLKVEALDEILSFLSHFQDAEDEAIDLLLDELENESLKSSILDKES 65

Query: 67  VHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFL-HTENLPIHGD 126
           VHRV+S+++ A+   EE+P  I++ SAL I++AF + KF YDPIKKIF  +T  L IHGD
Sbjct: 66  VHRVVSLLLEAEAAVEETPGAISTRSALRIVDAFLMSKFCYDPIKKIFYENTGRLAIHGD 125

Query: 127 ASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMGV 186
           ASAK+ALYR+RFLLL QRLSRDQHFSKPAFD  MSHFGSCEISPIQSL+GQTG++WVMGV
Sbjct: 126 ASAKSALYRNRFLLLFQRLSRDQHFSKPAFDTEMSHFGSCEISPIQSLIGQTGKRWVMGV 185

Query: 187 ISQMEDGHFYLEDLTASVEINLSSAISFT 215
           ISQ+EDGHF+LEDLTASVEINLS+AISFT
Sbjct: 186 ISQLEDGHFFLEDLTASVEINLSNAISFT 213

[Close]

BLAST of Csa1G434690 vs. NCBI nr
Match: gi|225455720|ref|XP_002267181.1| (PREDICTED: DNA polymerase epsilon subunit 2 [Vitis vinifera])

HSP 1 Score: 283.5 bits (724), Expect = 3.3e-73
Identity = 148/212 (69.81%), Postives = 177/212 (83.49%), Query Frame = 1


Query: 7   RKKVQRKAKIRGLYSIKLEALDEIVSFVSRSHGFEDEAIEFVLDNLHEESLKSPILDKDA 66
           RKKVQRK KIRG Y++K+EALDEI+SF+S     EDEAI+ +LD L  ESLKS ILDK++
Sbjct: 6   RKKVQRKFKIRG-YTLKVEALDEILSFLSHFQDAEDEAIDLLLDELENESLKSSILDKES 65

Query: 67  VHRVISIMVAADKVGEESPNTITSTSALCIINAFDIPKFRYDPIKKIFL-HTENLPIHGD 126
           VHRV+S+++ A+   EE+P  I++ SAL I++AF + KF YDPIKKIF  +T  L IHGD
Sbjct: 66  VHRVVSLLLEAEAAVEETPGAISTRSALRIVDAFLMSKFCYDPIKKIFYENTGRLAIHGD 125

Query: 127 ASAKAALYRDRFLLLSQRLSRDQHFSKPAFDIGMSHFGSCEISPIQSLVGQTGRKWVMGV 186
           ASAK+ALYR+RFLLL QRLSRDQHFSKPAFD  MSHFGSCEISPIQSL+GQTG++WVMGV
Sbjct: 126 ASAKSALYRNRFLLLFQRLSRDQHFSKPAFDTEMSHFGSCEISPIQSLIGQTGKRWVMGV 185

Query: 187 ISQMEDGHFYLEDLTASVEINLSSAISFTGNF 218
           ISQ+EDGHF+LEDLTASVEINLS+A   TG F
Sbjct: 186 ISQLEDGHFFLEDLTASVEINLSNAKITTGFF 216

The following BLAST results are available for this feature:

BLAST of Csa1G434690 vs. Swiss-Prot


Match Name	E-value	Identity	Description
DPB2_ARATH	1.8e-55	57.75	DNA polymerase epsilon subunit B OS=Arabidopsis thaliana GN=DPB2 PE=1 SV=1	[more]
DPOE2_DICDI	5.8e-22	32.85	DNA polymerase epsilon subunit 2 OS=Dictyostelium discoideum GN=pole2 PE=3 SV=1	[more]
DPOE2_BOVIN	8.2e-16	30.00	DNA polymerase epsilon subunit 2 OS=Bos taurus GN=POLE2 PE=2 SV=1	[more]
DPOE2_CHICK	2.0e-14	27.63	DNA polymerase epsilon subunit 2 OS=Gallus gallus GN=POLE2 PE=2 SV=1	[more]
DPOE2_MOUSE	2.0e-14	29.09	DNA polymerase epsilon subunit 2 OS=Mus musculus GN=Pole2 PE=2 SV=2	[more]

BLAST of Csa1G434690 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LUV9_CUCSA	5.9e-122	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G434690 PE=4 SV=1	[more]
A5AMH8_VITVI	9.2e-75	71.29	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_038180 PE=4 SV=1	[more]
A0A161ZS40_DAUCA	1.4e-70	64.62	Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_023267 PE=4 SV=1	[more]
A0A059AW13_EUCGR	3.1e-70	65.58	DNA polymerase epsilon subunit 2 OS=Eucalyptus grandis GN=EUGRSUZ_H00594 PE=3 SV...	[more]
D7SIP4_VITVI	3.4e-69	66.20	DNA polymerase epsilon subunit 2 OS=Vitis vinifera GN=VIT_17s0000g04830 PE=3 SV=...	[more]

BLAST of Csa1G434690 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G22110.1	1.0e-56	57.75	DNA polymerase epsilon subunit B2	[more]

BLAST of Csa1G434690 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700210427\|gb\|KGN65523.1\|	8.5e-122	100.00	hypothetical protein Csa_1G434690 [Cucumis sativus]	[more]
gi\|778660923\|ref\|XP_011657177.1\|	3.2e-113	98.16	PREDICTED: DNA polymerase epsilon subunit 2 [Cucumis sativus]	[more]
gi\|659066275\|ref\|XP_008463560.1\|	1.3e-98	87.16	PREDICTED: LOW QUALITY PROTEIN: DNA polymerase epsilon subunit 2 [Cucumis melo]	[more]
gi\|147818232\|emb\|CAN73546.1\|	1.3e-74	71.29	hypothetical protein VITISV_038180 [Vitis vinifera]	[more]
gi\|225455720\|ref\|XP_002267181.1\|	3.3e-73	69.81	PREDICTED: DNA polymerase epsilon subunit 2 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071897	DNA biosynthetic process
biological_process	GO:0006261	DNA-dependent DNA replication
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0051781	positive regulation of cell division
cellular_component	GO:0008622	epsilon DNA polymerase complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003887	DNA-directed DNA polymerase activity
molecular_function	GO:0070182	DNA polymerase binding

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
CU103566	cucumber EST collection version 3.0	transcribed_cluster
CU129897	cucumber EST collection version 3.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csa1G434690.1	Csa1G434690.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
CU103566	CU103566	transcribed_cluster
CU129897	CU129897	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR12708	DNA POLYMERASE EPSILON SUBUNIT B	coord: 1..218 score: 2.9
None	No IPR available	PANTHER	PTHR12708:SF0	DNA POLYMERASE EPSILON SUBUNIT 2	coord: 1..218 score: 2.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Csa1G434690	CSPI01G20940	Wild cucumber (PI 183967)	cpicuB001
Csa1G434690	Cucsa.054690	Cucumber (Gy14) v1	cgycuB039
Csa1G434690	CsGy1G020700	Cucumber (Gy14) v2	cgybcuB002

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None