WMU79022 (transcribed_cluster) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	WMU79022
Type	transcribed_cluster
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Alpha-galactosidase
Location	Chr11 : 15266669 .. 15270162 (+)
Sequence length	560

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

CAACATCGGATATTCAAGATAACTGGACTAGCATGACAACCATTGCTGATCAGAATGACAAATGGGCTTCTTACGCAAAACCAGGAGGATGGAATGATCCCGACATGCTTAAGTGGGGAACGGAGGGATGACCACAGTAGAATACCGTTCTCATTTTAGCATTTGGGCACTGGCAAAAGCTCCATTGCTGATTGGATGTGACATCAGATCAATGGACAACGTCACCTTGAAATTGTTAAGTAATAAGGAGGTTATTGCAGTTAATCAGGATAAGCTTGGAGTCCAAGGCAAGAAGGTCTATAAATATGGAGACCTCGAGGTTTGGGCAGGGCCGCTGAGTGGTAAAAGAGTCGCCGTGGTTCTATGGAACAGAGGTTTGTGGCGAGCCAATATCACTGCATCTTGGTCTGATATAGGTTTGAGTCCATCAACTGTAGTTACTGCTCGAGACTTATGGGAGCACTCCAGGCTGGTGGTTCAACATCACCTTACTGCTGAAGTAGACTCTCATGGCTGTAAGATGTACGTTCTCACACCCCACTAAGCCAAAACTCGTAATT

Library


Library category	ESTs
Watermelon normalization and subtraction library	1

Homology

[Close]

BLAST of WMU79022 vs. TAIR10
Match: AT5G08370.1 (AT5G08370.1 alpha-galactosidase 2)

HSP 1 Score: 213.0 bits (541), Expect = 1.6e-55
Identity = 106/142 (74.65%), Postives = 115/142 (80.99%), Query Frame = 2


Query: 113 VGNGGMTTVEYRSHFSIWALAKAPLLIGCDIRSMDNVTLKLLSNKEVIAVNQDKLGVQGK 172
           VGNGGMT  EY SHFSIWALAKAPLLIGCD+RSMD VT +LLSNKEVIAVNQDKLG+QGK
Sbjct: 251 VGNGGMTKEEYMSHFSIWALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGK 310

Query: 173 KVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSPSTVVTARDLWEHSRL 232
           KV K GDLEVWAGPLS KRVAV+LWNRG   ANITA W++IGL+ S +V ARDLWEHS  
Sbjct: 311 KVKKEGDLEVWAGPLSKKRVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTY 370

Query: 233 -VVQHHLTAEVDSHGCKMYVLT 292
             V+  L+A V+ H CKMY LT
Sbjct: 371 SCVKKQLSALVEPHACKMYTLT 392

Query: 293  352
           
Sbjct: 431  392

Query: 353  412
           
Sbjct: 491  392

Query: 413  472
           
Sbjct: 551  392

Query: 473  532
           
Sbjct: 611  392

Query: 533  536
           
Sbjct: 671  392

HSP 2 Score: 76.3 bits (186), Expect = 2.3e-14
Identity = 32/42 (76.19%), Postives = 33/42 (78.57%), Query Frame = 3


Query: 3   TSDIQDNWTSMTTIADQNDKWASYAKPGGWNDPDMLKWGTEG 62
           T DIQDNW SMT IADQND+WASYA+PG WNDPDML+ G  G
Sbjct: 214 TGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGG 255

Query: 63   122
           
Sbjct: 274  255

Query: 123  129
           
Sbjct: 334  255

[Close]

BLAST of WMU79022 vs. TAIR10
Match: AT5G08380.1 (AT5G08380.1 alpha-galactosidase 1)

HSP 1 Score: 181.0 bits (458), Expect = 6.6e-46
Identity = 90/144 (62.50%), Postives = 108/144 (75.00%), Query Frame = 2


Query: 113 VGNGGMTTVEYRSHFSIWALAKAPLLIGCDIRSMDNVTLKLLSNKEVIAVNQDKLGVQGK 172
           VGNGGMT  EY  HFSIWA++KAPLL+GCDIR+M   T+++++NKEVIA+NQD  GVQ K
Sbjct: 265 VGNGGMTKDEYIVHFSIWAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAK 324

Query: 173 KVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSPSTVVTARDLWEHSRL 232
           KV   GDLEVWAGPLSG RVA++L NRG  R +ITA W DI +  +++V ARDLWEH  L
Sbjct: 325 KVRMEGDLEVWAGPLSGYRVALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEHQTL 384

Query: 233 VVQH--HLTAEVDSHGCKMYVLTP 292
             +   +LTA VDSH CK+YVL P
Sbjct: 385 KQKFVGNLTATVDSHACKLYVLKP 408

Query: 293  352
           
Sbjct: 445  408

Query: 353  412
           
Sbjct: 505  408

Query: 413  472
           
Sbjct: 565  408

Query: 473  532
           
Sbjct: 625  408

Query: 533  539
           
Sbjct: 685  408

HSP 2 Score: 61.6 bits (148), Expect = 5.8e-10
Identity = 25/42 (59.52%), Postives = 31/42 (73.81%), Query Frame = 3


Query: 3   TSDIQDNWTSMTTIADQNDKWASYAKPGGWNDPDMLKWGTEG 62
           T+DI+D W SM +IAD N+ +A +A+PGGWNDPDML+ G  G
Sbjct: 228 TNDIKDTWLSMISIADMNEVYAEHARPGGWNDPDMLEVGNGG 269

Query: 63   122
           
Sbjct: 288  269

Query: 123  129
           
Sbjct: 348  269

[Close]

BLAST of WMU79022 vs. TAIR10
Match: AT3G56310.1 (AT3G56310.1 Melibiase family protein)

HSP 1 Score: 172.2 bits (435), Expect = 3.1e-43
Identity = 86/147 (58.50%), Postives = 102/147 (69.39%), Query Frame = 2


Query: 113 VGNGGMTTVEYRSHFSIWALAKAPLLIGCDIRSMDNVTLKLLSNKEVIAVNQDKLGVQGK 172
           +GNGGMT  EYR HFSIWAL KAPLLIGCD+R+M   TL++LSNKE+IAVNQD LGVQG+
Sbjct: 283 IGNGGMTYEEYRGHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGR 342

Query: 173 KVYKYGD---LEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSPSTVVTARDLWEH 232
           K+   G+    +VW+GPLSG R+ V LWNR    A ITASW  IGL  +  V+ RDLW+H
Sbjct: 343 KIQANGENDCQQVWSGPLSGDRMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQH 402

Query: 233 SRLV--VQHHLTAEVDSHGCKMYVLTP 292
             +         A+VD+H C MYVLTP
Sbjct: 403 KDVTENTSGSFEAQVDAHDCHMYVLTP 429

Query: 293  352
           
Sbjct: 463  429

Query: 353  412
           
Sbjct: 523  429

Query: 413  472
           
Sbjct: 583  429

Query: 473  532
           
Sbjct: 643  429

Query: 533  539
           
Sbjct: 703  429

HSP 2 Score: 70.9 bits (172), Expect = 9.6e-13
Identity = 30/42 (71.43%), Postives = 31/42 (73.81%), Query Frame = 3


Query: 3   TSDIQDNWTSMTTIADQNDKWASYAKPGGWNDPDMLKWGTEG 62
           T DI D W SMTTIAD N+KWA+YA PGGWNDPDML+ G  G
Sbjct: 246 TDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGG 287

Query: 63   122
           
Sbjct: 306  287

Query: 123  129
           
Sbjct: 366  287

[Close]

BLAST of WMU79022 vs. Swiss-Prot
Match: AGAL_CYATE (Alpha-galactosidase OS=Cyamopsis tetragonoloba PE=1 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 4.3e-55
Identity = 105/143 (73.43%), Postives = 116/143 (81.12%), Query Frame = 2


Query: 113 VGNGGMTTVEYRSHFSIWALAKAPLLIGCDIRSMDNVTLKLLSNKEVIAVNQDKLGVQGK 172
           VGNGGMTT EYRSHFSIWALAKAPLL+GCDIR+MD+ T +L+SN EVIAVNQDKLGVQGK
Sbjct: 267 VGNGGMTTEEYRSHFSIWALAKAPLLVGCDIRAMDDTTHELISNAEVIAVNQDKLGVQGK 326

Query: 173 KVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSPSTVVTARDLWEHS-R 232
           KV    DLEVWAGPLS  +VAV+LWNR   RA +TASWSDIGL   T V ARDLWEHS +
Sbjct: 327 KVKSTNDLEVWAGPLSDNKVAVILWNRSSSRATVTASWSDIGLQQGTTVDARDLWEHSTQ 386

Query: 233 LVVQHHLTAEVDSHGCKMYVLTP 292
            +V   ++AE+DSH CKMYVLTP
Sbjct: 387 SLVSGEISAEIDSHACKMYVLTP 409

Query: 293  352
           
Sbjct: 447  409

Query: 353  412
           
Sbjct: 507  409

Query: 413  472
           
Sbjct: 567  409

Query: 473  532
           
Sbjct: 627  409

Query: 533  539
           
Sbjct: 687  409

HSP 2 Score: 76.3 bits (186), Expect = 4.1e-13
Identity = 32/42 (76.19%), Postives = 33/42 (78.57%), Query Frame = 3


Query: 3   TSDIQDNWTSMTTIADQNDKWASYAKPGGWNDPDMLKWGTEG 62
           T DI+DNW SMT+IAD NDKWASYA PGGWNDPDML+ G  G
Sbjct: 230 TGDIEDNWNSMTSIADSNDKWASYAGPGGWNDPDMLEVGNGG 271

Query: 63   122
           
Sbjct: 290  271

Query: 123  129
           
Sbjct: 350  271

[Close]

BLAST of WMU79022 vs. Swiss-Prot
Match: AGAL_COFAR (Alpha-galactosidase OS=Coffea arabica PE=1 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 9.6e-55
Identity = 108/143 (75.52%), Postives = 113/143 (79.02%), Query Frame = 2


Query: 113 VGNGGMTTVEYRSHFSIWALAKAPLLIGCDIRSMDNVTLKLLSNKEVIAVNQDKLGVQGK 172
           VGNGGMTT EYRSHFSIWALAKAPLLIGCDIRSMD  T +LLSN EVIAVNQDKLGVQG 
Sbjct: 235 VGNGGMTTTEYRSHFSIWALAKAPLLIGCDIRSMDGATFQLLSNAEVIAVNQDKLGVQGN 294

Query: 173 KVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSPSTVVTARDLWEHS-R 232
           KV  YGDLEVWAGPLSGKRVAV LWNRG   A ITA WSD+GL  + VV ARDLW HS  
Sbjct: 295 KVKTYGDLEVWAGPLSGKRVAVALWNRGSSTATITAYWSDVGLPSTAVVNARDLWAHSTE 354

Query: 233 LVVQHHLTAEVDSHGCKMYVLTP 292
             V+  ++A VD+H  KMYVLTP
Sbjct: 355 KSVKGQISAAVDAHDSKMYVLTP 377

Query: 293  352
           
Sbjct: 415  377

Query: 353  412
           
Sbjct: 475  377

Query: 413  472
           
Sbjct: 535  377

Query: 473  532
           
Sbjct: 595  377

Query: 533  539
           
Sbjct: 655  377

HSP 2 Score: 71.2 bits (173), Expect = 1.3e-11
Identity = 30/42 (71.43%), Postives = 32/42 (76.19%), Query Frame = 3


Query: 3   TSDIQDNWTSMTTIADQNDKWASYAKPGGWNDPDMLKWGTEG 62
           T DI D+W+SMT+ AD NDKWASYA PGGWNDPDML+ G  G
Sbjct: 198 TGDIDDSWSSMTSRADMNDKWASYAGPGGWNDPDMLEVGNGG 239

Query: 63   122
           
Sbjct: 258  239

Query: 123  129
           
Sbjct: 318  239

[Close]

BLAST of WMU79022 vs. Swiss-Prot
Match: AGAL2_ARATH (Alpha-galactosidase 2 OS=Arabidopsis thaliana GN=AGAL2 PE=1 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 2.8e-54
Identity = 106/142 (74.65%), Postives = 115/142 (80.99%), Query Frame = 2


Query: 113 VGNGGMTTVEYRSHFSIWALAKAPLLIGCDIRSMDNVTLKLLSNKEVIAVNQDKLGVQGK 172
           VGNGGMT  EY SHFSIWALAKAPLLIGCD+RSMD VT +LLSNKEVIAVNQDKLG+QGK
Sbjct: 251 VGNGGMTKEEYMSHFSIWALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGK 310

Query: 173 KVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSPSTVVTARDLWEHSRL 232
           KV K GDLEVWAGPLS KRVAV+LWNRG   ANITA W++IGL+ S +V ARDLWEHS  
Sbjct: 311 KVKKEGDLEVWAGPLSKKRVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTY 370

Query: 233 -VVQHHLTAEVDSHGCKMYVLT 292
             V+  L+A V+ H CKMY LT
Sbjct: 371 SCVKKQLSALVEPHACKMYTLT 392

Query: 293  352
           
Sbjct: 431  392

Query: 353  412
           
Sbjct: 491  392

Query: 413  472
           
Sbjct: 551  392

Query: 473  532
           
Sbjct: 611  392

Query: 533  536
           
Sbjct: 671  392

HSP 2 Score: 76.3 bits (186), Expect = 4.1e-13
Identity = 32/42 (76.19%), Postives = 33/42 (78.57%), Query Frame = 3


Query: 3   TSDIQDNWTSMTTIADQNDKWASYAKPGGWNDPDMLKWGTEG 62
           T DIQDNW SMT IADQND+WASYA+PG WNDPDML+ G  G
Sbjct: 214 TGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGG 255

Query: 63   122
           
Sbjct: 274  255

Query: 123  129
           
Sbjct: 334  255

[Close]

BLAST of WMU79022 vs. Swiss-Prot
Match: AGAL_ORYSJ (Alpha-galactosidase OS=Oryza sativa subsp. japonica GN=Os10g0493600 PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 3.2e-50
Identity = 98/143 (68.53%), Postives = 106/143 (74.13%), Query Frame = 2


Query: 113 VGNGGMTTVEYRSHFSIWALAKAPLLIGCDIRSMDNVTLKLLSNKEVIAVNQDKLGVQGK 172
           VGNGGM+  EYRSHFSIWALAKAPLLIGCD+RSM   T  +LSN EVIAVNQD LGVQGK
Sbjct: 275 VGNGGMSEAEYRSHFSIWALAKAPLLIGCDVRSMSQQTKNILSNSEVIAVNQDSLGVQGK 334

Query: 173 KVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSPSTVVTARDLWEHSRL 232
           KV     LEVWAGPLS  R AVVLWNR  ++A ITA WS+IGL+ S  VTARDLW HS  
Sbjct: 335 KVQSDNGLEVWAGPLSNNRKAVVLWNRQSYQATITAHWSNIGLAGSVAVTARDLWAHSSF 394

Query: 233 VVQHHLTAEVDSHGCKMYVLTPH 292
             Q  ++A V  H CKMYVLTP+
Sbjct: 395 AAQGQISASVAPHDCKMYVLTPN 417

Query: 293  352
           
Sbjct: 455  417

Query: 353  412
           
Sbjct: 515  417

Query: 413  472
           
Sbjct: 575  417

Query: 473  532
           
Sbjct: 635  417

Query: 533  542
           
Sbjct: 695  417

HSP 2 Score: 69.7 bits (169), Expect = 3.8e-11
Identity = 29/42 (69.05%), Postives = 32/42 (76.19%), Query Frame = 3


Query: 3   TSDIQDNWTSMTTIADQNDKWASYAKPGGWNDPDMLKWGTEG 62
           T DI DNW SMT+ AD+ND+WA+YA PGGWNDPDML+ G  G
Sbjct: 238 TGDIADNWGSMTSRADENDQWAAYAGPGGWNDPDMLEVGNGG 279

Query: 63   122
           
Sbjct: 298  279

Query: 123  129
           
Sbjct: 358  279

[Close]

BLAST of WMU79022 vs. Swiss-Prot
Match: AGAL1_ARATH (Alpha-galactosidase 1 OS=Arabidopsis thaliana GN=AGAL1 PE=2 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.2e-44
Identity = 90/144 (62.50%), Postives = 108/144 (75.00%), Query Frame = 2


Query: 113 VGNGGMTTVEYRSHFSIWALAKAPLLIGCDIRSMDNVTLKLLSNKEVIAVNQDKLGVQGK 172
           VGNGGMT  EY  HFSIWA++KAPLL+GCDIR+M   T+++++NKEVIA+NQD  GVQ K
Sbjct: 265 VGNGGMTKDEYIVHFSIWAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAK 324

Query: 173 KVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSPSTVVTARDLWEHSRL 232
           KV   GDLEVWAGPLSG RVA++L NRG  R +ITA W DI +  +++V ARDLWEH  L
Sbjct: 325 KVRMEGDLEVWAGPLSGYRVALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEHQTL 384

Query: 233 VVQH--HLTAEVDSHGCKMYVLTP 292
             +   +LTA VDSH CK+YVL P
Sbjct: 385 KQKFVGNLTATVDSHACKLYVLKP 408

Query: 293  352
           
Sbjct: 445  408

Query: 353  412
           
Sbjct: 505  408

Query: 413  472
           
Sbjct: 565  408

Query: 473  532
           
Sbjct: 625  408

Query: 533  539
           
Sbjct: 685  408

HSP 2 Score: 61.6 bits (148), Expect = 1.0e-08
Identity = 25/42 (59.52%), Postives = 31/42 (73.81%), Query Frame = 3


Query: 3   TSDIQDNWTSMTTIADQNDKWASYAKPGGWNDPDMLKWGTEG 62
           T+DI+D W SM +IAD N+ +A +A+PGGWNDPDML+ G  G
Sbjct: 228 TNDIKDTWLSMISIADMNEVYAEHARPGGWNDPDMLEVGNGG 269

Query: 63   122
           
Sbjct: 288  269

Query: 123  129
           
Sbjct: 348  269

[Close]

BLAST of WMU79022 vs. NCBI nr
Match: gi|659090245|ref|XP_008445911.1| (PREDICTED: alpha-galactosidase [Cucumis melo])

HSP 1 Score: 279.6 bits (714), Expect = 3.9e-72
Identity = 131/143 (91.61%), Postives = 136/143 (95.10%), Query Frame = 2


Query: 113 VGNGGMTTVEYRSHFSIWALAKAPLLIGCDIRSMDNVTLKLLSNKEVIAVNQDKLGVQGK 172
           VGNGGMTT EYRSHFSIWALAKAPLLIGCDIRSMDN+T+KLLSNKEVIAVNQDKLGVQGK
Sbjct: 277 VGNGGMTTAEYRSHFSIWALAKAPLLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGK 336

Query: 173 KVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSPSTVVTARDLWEHSRL 232
           KVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGL  ST VTARDLW+HS  
Sbjct: 337 KVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLCSSTTVTARDLWQHSSQ 396

Query: 233 VVQHHLTAEVDSHGCKMYVLTPH 292
           VVQHHLTA+VDSH CKM+VLTPH
Sbjct: 397 VVQHHLTAQVDSHDCKMFVLTPH 419

Query: 293  352
           
Sbjct: 457  419

Query: 353  412
           
Sbjct: 517  419

Query: 413  472
           
Sbjct: 577  419

Query: 473  532
           
Sbjct: 637  419

Query: 533  542
           
Sbjct: 697  419

[Close]

BLAST of WMU79022 vs. NCBI nr
Match: gi|525506969|ref|NP_001267530.1| (alpha-galactosidase-like precursor [Cucumis sativus])

HSP 1 Score: 266.2 bits (679), Expect = 4.4e-68
Identity = 127/143 (88.81%), Postives = 132/143 (92.31%), Query Frame = 2


Query: 113 VGNGGMTTVEYRSHFSIWALAKAPLLIGCDIRSMDNVTLKLLSNKEVIAVNQDKLGVQGK 172
           VGNGGMT  EYRSHFSIWALAKAPLLIGCDIRSMDN T+KLLSNKEVIAVNQDKLGVQGK
Sbjct: 279 VGNGGMTIAEYRSHFSIWALAKAPLLIGCDIRSMDNNTMKLLSNKEVIAVNQDKLGVQGK 338

Query: 173 KVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSPSTVVTARDLWEHSRL 232
           KV+KYGDLEVWAG LSGKRVAVVLWNR LWRANITA WSDIGLS ST VTARDLWEHS  
Sbjct: 339 KVHKYGDLEVWAGLLSGKRVAVVLWNRSLWRANITAYWSDIGLSSSTTVTARDLWEHSSQ 398

Query: 233 VVQHHLTAEVDSHGCKMYVLTPH 292
           VV+HHLTA+VDSH CKM+VLTPH
Sbjct: 399 VVRHHLTAQVDSHDCKMFVLTPH 421

Query: 293  352
           
Sbjct: 459  421

Query: 353  412
           
Sbjct: 519  421

Query: 413  472
           
Sbjct: 579  421

Query: 473  532
           
Sbjct: 639  421

Query: 533  542
           
Sbjct: 699  421

[Close]

BLAST of WMU79022 vs. NCBI nr
Match: gi|823264980|ref|XP_012465230.1| (PREDICTED: alpha-galactosidase isoform X1 [Gossypium raimondii])

HSP 1 Score: 226.5 bits (576), Expect = 3.9e-56
Identity = 111/150 (74.00%), Postives = 121/150 (80.67%), Query Frame = 2


Query: 113 VGNGGMTTVEYRSHFSIWALAKAPLLIGCDIRSMDNVTLKLLSNKEVIAVNQDKLGVQGK 172
           VGNGGMTT EYR HFSIWALAKAPLLIGCD+RSMDNVT +L++NKEVI VNQDKLGVQGK
Sbjct: 264 VGNGGMTTEEYRCHFSIWALAKAPLLIGCDVRSMDNVTFELVANKEVIDVNQDKLGVQGK 323

Query: 173 KVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSPSTVVTARDLWEHS-R 232
           KV K GDLEVWAGPL+   VAVVLWNRG   ANITA WSDIGL PSTVV  RDLW HS  
Sbjct: 324 KVKKEGDLEVWAGPLANNMVAVVLWNRGSSSANITAYWSDIGLKPSTVVDCRDLWAHSTE 383

Query: 233 LVVQHHLTAEVDSHGCKMYVLTPH*AKTRN 292
             V+  ++AEVDSH CKMY L PH  + R+
Sbjct: 384 TGVEDEISAEVDSHACKMYTLKPHTNRDRS 413

Query: 293  352
           
Sbjct: 444  413

Query: 353  412
           
Sbjct: 504  413

Query: 413  472
           
Sbjct: 564  413

Query: 473  532
           
Sbjct: 624  413

Query: 533  560
           
Sbjct: 684  413

[Close]

BLAST of WMU79022 vs. NCBI nr
Match: gi|590615546|ref|XP_007023252.1| (Alpha-galactosidase 2 [Theobroma cacao])

HSP 1 Score: 226.5 bits (576), Expect = 3.9e-56
Identity = 111/143 (77.62%), Postives = 120/143 (83.92%), Query Frame = 2


Query: 113 VGNGGMTTVEYRSHFSIWALAKAPLLIGCDIRSMDNVTLKLLSNKEVIAVNQDKLGVQGK 172
           VGNGGMTT EYRSHFSIW+LAKAPLLIGCDIRSMDNVT +LLSNKEVIAVNQDKLGVQGK
Sbjct: 268 VGNGGMTTEEYRSHFSIWSLAKAPLLIGCDIRSMDNVTFELLSNKEVIAVNQDKLGVQGK 327

Query: 173 KVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSPSTVVTARDLWEHS-R 232
           KV K GDLEVWAGPL+  RVAVVLWNRG   ANITA WSDIGL PSTV   + LW HS  
Sbjct: 328 KVKKDGDLEVWAGPLTDNRVAVVLWNRGSSSANITAYWSDIGLKPSTVCDVQHLWAHSTE 387

Query: 233 LVVQHHLTAEVDSHGCKMYVLTP 292
           L VQ  L+A+VD+H C+MY +TP
Sbjct: 388 LSVQDQLSAQVDAHACRMYTITP 410

Query: 293  352
           
Sbjct: 448  410

Query: 353  412
           
Sbjct: 508  410

Query: 413  472
           
Sbjct: 568  410

Query: 473  532
           
Sbjct: 628  410

Query: 533  539
           
Sbjct: 688  410

[Close]

BLAST of WMU79022 vs. NCBI nr
Match: gi|729361561|ref|XP_010546252.1| (PREDICTED: alpha-galactosidase [Tarenaya hassleriana])

HSP 1 Score: 225.3 bits (573), Expect = 8.7e-56
Identity = 110/142 (77.46%), Postives = 121/142 (85.21%), Query Frame = 2


Query: 113 VGNGGMTTVEYRSHFSIWALAKAPLLIGCDIRSMDNVTLKLLSNKEVIAVNQDKLGVQGK 172
           VGNGGMTT EYRSHFSIWALAKAPLLIGCDIRSMD +T KLLSNKEVIAVNQDKLGVQGK
Sbjct: 255 VGNGGMTTEEYRSHFSIWALAKAPLLIGCDIRSMDKLTFKLLSNKEVIAVNQDKLGVQGK 314

Query: 173 KVYKYGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLSPSTVVTARDLWEHS-R 232
           KV K GDLEVWAGPL+ KRVA+VLWNRG  +ANITA W+DIGL  STVV  RDLWEHS +
Sbjct: 315 KVKKEGDLEVWAGPLTKKRVAIVLWNRGESQANITARWTDIGLGSSTVVDVRDLWEHSTK 374

Query: 233 LVVQHHLTAEVDSHGCKMYVLT 292
             V++ ++A V+ H CKMY LT
Sbjct: 375 SAVKYQISALVEPHACKMYTLT 396

Query: 293  352
           
Sbjct: 435  396

Query: 353  412
           
Sbjct: 495  396

Query: 413  472
           
Sbjct: 555  396

Query: 473  532
           
Sbjct: 615  396

Query: 533  536
           
Sbjct: 675  396

The following BLAST results are available for this feature:

BLAST of WMU79022 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G08370.1	1.6e-55	74.65	alpha-galactosidase 2	[more]
AT5G08380.1	6.6e-46	62.50	alpha-galactosidase 1	[more]
AT3G56310.1	3.1e-43	58.50	Melibiase family protein	[more]

BLAST of WMU79022 vs. Swiss-Prot


Match Name	E-value	Identity	Description
AGAL_CYATE	4.3e-55	73.43	Alpha-galactosidase OS=Cyamopsis tetragonoloba PE=1 SV=1	[more]
AGAL_COFAR	9.6e-55	75.52	Alpha-galactosidase OS=Coffea arabica PE=1 SV=1	[more]
AGAL2_ARATH	2.8e-54	74.65	Alpha-galactosidase 2 OS=Arabidopsis thaliana GN=AGAL2 PE=1 SV=1	[more]
AGAL_ORYSJ	3.2e-50	68.53	Alpha-galactosidase OS=Oryza sativa subsp. japonica GN=Os10g0493600 PE=1 SV=1	[more]
AGAL1_ARATH	1.2e-44	62.50	Alpha-galactosidase 1 OS=Arabidopsis thaliana GN=AGAL1 PE=2 SV=1	[more]

BLAST of WMU79022 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659090245\|ref\|XP_008445911.1\|	3.9e-72	91.61	PREDICTED: alpha-galactosidase [Cucumis melo]	[more]
gi\|525506969\|ref\|NP_001267530.1\|	4.4e-68	88.81	alpha-galactosidase-like precursor [Cucumis sativus]	[more]
gi\|823264980\|ref\|XP_012465230.1\|	3.9e-56	74.00	PREDICTED: alpha-galactosidase isoform X1 [Gossypium raimondii]	[more]
gi\|590615546\|ref\|XP_007023252.1\|	3.9e-56	77.62	Alpha-galactosidase 2 [Theobroma cacao]	[more]
gi\|729361561\|ref\|XP_010546252.1\|	8.7e-56	77.46	PREDICTED: alpha-galactosidase [Tarenaya hassleriana]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR002241	Glyco_hydro_27
IPR013780	Glyco_hydro_b
IPR013785	Aldolase_TIM
IPR017853	Glycoside_hydrolase_SF

Vocabulary: **Molecular Function**
Term	Definition
GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Cla022885	Cla022885	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
WMN09F10	WMN09F10	EST

InterPro

Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002241	Glycoside hydrolase, family 27	PRINTS	PR00740	GLHYDRLASE27	coord: 26..45 score: 1.0E-14coord: 47..68 score: 1.0
IPR002241	Glycoside hydrolase, family 27	PFAM	PF16499	Melibiase_2	coord: 3..156 score: 1.2
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	G3DSA:2.60.40.1180		coord: 86..180 score: 6.6
IPR013785	Aldolase-type TIM barrel	GENE3D	G3DSA:3.20.20.70		coord: 2..85 score: 2.0
IPR017853	Glycoside hydrolase superfamily	unknown	SSF51445	(Trans)glycosidases	coord: 2..91 score: 5.48
None	No IPR available	PANTHER	PTHR11452	ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE	coord: 2..180 score: 5.7E
None	No IPR available	PANTHER	PTHR11452:SF33	SUBFAMILY NOT NAMED	coord: 2..180 score: 5.7E
None	No IPR available	unknown	SSF51011	Glycosyl hydrolase domain	coord: 86..179 score: 2.34