WMU78668 (transcribed_cluster) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	WMU78668
Type	transcribed_cluster
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Glutathione S-transferase
Location	Chr5 : 3378773 .. 3378998 (+)
Sequence length	225

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

CTCCATTGAAGATGACAGAAGTGAAGCTCCATGGAGCTTGGCCAAGTCCATTCAGTTACAGAGTCATTTGGGCTCTGGCTCTCAAAGGCATTCCATACCAATACATAGAAGAGGATCTCGCCAACAAATCTCCTCTGCTTTTGCAATACAATCCCGTTCACAAGAAGATTCCAGTCCTCGTCCATGCCGGAAAACCCATCTGCGAATCCTCCATCATCCTCCAGT

Library


Library category	ESTs
watermelon fusarium SSH library	1

Homology

[Close]

BLAST of WMU78668 vs. TAIR10
Match: AT2G29450.1 (AT2G29450.1 glutathione S-transferase tau 5)

HSP 1 Score: 98.6 bits (244), Expect = 1.7e-21
Identity = 48/69 (69.57%), Postives = 52/69 (75.36%), Query Frame = 3


Query: 18  EVKLHGAWPSPFSYRVIWALALKGIPYQYIEEDLANKSPLLLQYNPVHKKIPVLVHAGKP 77
           EVKL G W SPFS RV  AL LKGIPY+Y+EE L NKSPLLL  NP+HKK+PVLVH GK 
Sbjct: 6   EVKLLGIWASPFSRRVEMALKLKGIPYEYVEEILENKSPLLLALNPIHKKVPVLVHNGKT 65

Query: 78  ICESSIILQ 137
           I ES +IL+
Sbjct: 66  ILESHVILE 74

Query: 138  197
           
Sbjct: 126  74

Query: 198  225
           
Sbjct: 186  74

[Close]

BLAST of WMU78668 vs. TAIR10
Match: AT2G29440.1 (AT2G29440.1 glutathione S-transferase tau 6)

HSP 1 Score: 97.1 bits (240), Expect = 5.0e-21
Identity = 47/72 (65.28%), Postives = 53/72 (73.61%), Query Frame = 3


Query: 9   KMTEVKLHGAWPSPFSYRVIWALALKGIPYQYIEEDLANKSPLLLQYNPVHKKIPVLVHA 68
           K  EVKL G W SPFS R+  AL LKG+PY+Y+EEDL NKS LLL  +P+HKKIPVLVH 
Sbjct: 3   KNEEVKLLGIWASPFSRRIEMALKLKGVPYEYLEEDLENKSSLLLALSPIHKKIPVLVHN 62

Query: 69  GKPICESSIILQ 128
           GK I ES +IL+
Sbjct: 63  GKTIIESHVILE 74

Query: 129  188
           
Sbjct: 123  74

Query: 189  225
           
Sbjct: 183  74

[Close]

BLAST of WMU78668 vs. TAIR10
Match: AT2G29420.1 (AT2G29420.1 glutathione S-transferase tau 7)

HSP 1 Score: 94.7 bits (234), Expect = 2.5e-20
Identity = 45/69 (65.22%), Postives = 52/69 (75.36%), Query Frame = 3


Query: 18  EVKLHGAWPSPFSYRVIWALALKGIPYQYIEEDLANKSPLLLQYNPVHKKIPVLVHAGKP 77
           EVKL G W SPFS R+  AL LKG+ Y+++E+D+ NKS LLLQ NPVHK IPVLVH GKP
Sbjct: 9   EVKLLGMWASPFSRRIEIALTLKGVSYEFLEQDITNKSSLLLQLNPVHKMIPVLVHNGKP 68

Query: 78  ICESSIILQ 137
           I ES +IL+
Sbjct: 69  ISESLVILE 77

Query: 138  197
           
Sbjct: 129  77

Query: 198  225
           
Sbjct: 189  77

[Close]

BLAST of WMU78668 vs. TAIR10
Match: AT2G29490.1 (AT2G29490.1 glutathione S-transferase TAU 1)

HSP 1 Score: 94.4 bits (233), Expect = 3.2e-20
Identity = 45/72 (62.50%), Postives = 53/72 (73.61%), Query Frame = 3


Query: 9   KMTEVKLHGAWPSPFSYRVIWALALKGIPYQYIEEDLANKSPLLLQYNPVHKKIPVLVHA 68
           K   VKL G W SPFS RV  AL LKG+PY+Y+EEDL NK+PLLL+ NP+HKK+PVLVH 
Sbjct: 4   KEESVKLLGFWASPFSRRVEMALKLKGVPYEYLEEDLPNKTPLLLELNPLHKKVPVLVHN 63

Query: 69  GKPICESSIILQ 128
            K + ES +IL+
Sbjct: 64  DKILLESHLILE 75

Query: 129  188
           
Sbjct: 124  75

Query: 189  225
           
Sbjct: 184  75

[Close]

BLAST of WMU78668 vs. TAIR10
Match: AT5G62480.1 (AT5G62480.1 glutathione S-transferase tau 9)

HSP 1 Score: 93.2 bits (230), Expect = 7.2e-20
Identity = 44/69 (63.77%), Postives = 53/69 (76.81%), Query Frame = 3


Query: 18  EVKLHGAWPSPFSYRVIWALALKGIPYQYIEEDLANKSPLLLQYNPVHKKIPVLVHAGKP 77
           +V LHG++ SP+S R+  AL LK IPYQ+++EDL NKS  LL+YNPVHKKIPVLVH GKP
Sbjct: 8   KVILHGSFASPYSKRIELALRLKSIPYQFVQEDLQNKSQTLLRYNPVHKKIPVLVHNGKP 67

Query: 78  ICESSIILQ 137
           I ES  I++
Sbjct: 68  ISESLFIIE 76

Query: 138  197
           
Sbjct: 128  76

Query: 198  225
           
Sbjct: 188  76

[Close]

BLAST of WMU78668 vs. Swiss-Prot
Match: GSTX3_TOBAC (Probable glutathione S-transferase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 7.2e-22
Identity = 49/71 (69.01%), Postives = 55/71 (77.46%), Query Frame = 3


Query: 12  MTEVKLHGAWPSPFSYRVIWALALKGIPYQYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 71
           M EVKL G W SPF++RV WAL LKG+ Y+YIEED  NKS LLLQ NPVHKK+PVL+H G
Sbjct: 1   MAEVKLLGFWYSPFTHRVEWALKLKGVKYEYIEEDRDNKSSLLLQSNPVHKKVPVLIHNG 60

Query: 72  KPICESSIILQ 131
           KPI ES +IL+
Sbjct: 61  KPIVESMVILE 71

Query: 132  191
           
Sbjct: 121  71

Query: 192  225
           
Sbjct: 181  71

[Close]

BLAST of WMU78668 vs. Swiss-Prot
Match: GSTX1_SOLTU (Probable glutathione S-transferase OS=Solanum tuberosum GN=PRP1 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 9.5e-22
Identity = 49/71 (69.01%), Postives = 56/71 (78.87%), Query Frame = 3


Query: 12  MTEVKLHGAWPSPFSYRVIWALALKGIPYQYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 71
           M EVKL G   SPFS+RV WAL +KG+ Y++IEEDL NKSPLLLQ NP+HKKIPVL+H G
Sbjct: 1   MAEVKLLGLRYSPFSHRVEWALKIKGVKYEFIEEDLQNKSPLLLQSNPIHKKIPVLIHNG 60

Query: 72  KPICESSIILQ 131
           K ICES +IL+
Sbjct: 61  KCICESMVILE 71

Query: 132  191
           
Sbjct: 121  71

Query: 192  225
           
Sbjct: 181  71

[Close]

BLAST of WMU78668 vs. Swiss-Prot
Match: GSTX2_TOBAC (Probable glutathione S-transferase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 2.1e-21
Identity = 49/71 (69.01%), Postives = 55/71 (77.46%), Query Frame = 3


Query: 12  MTEVKLHGAWPSPFSYRVIWALALKGIPYQYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 71
           M EVKL G W SPFS+RV WAL +KG+ Y+YIEED  NKS LLLQ NPV+KK+PVL+H G
Sbjct: 1   MAEVKLLGFWYSPFSHRVEWALKIKGVKYEYIEEDRDNKSSLLLQSNPVYKKVPVLIHNG 60

Query: 72  KPICESSIILQ 131
           KPI ES IIL+
Sbjct: 61  KPIVESMIILE 71

Query: 132  191
           
Sbjct: 121  71

Query: 192  225
           
Sbjct: 181  71

[Close]

BLAST of WMU78668 vs. Swiss-Prot
Match: GSTX1_TOBAC (Probable glutathione S-transferase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 2.3e-20
Identity = 47/71 (66.20%), Postives = 53/71 (74.65%), Query Frame = 3


Query: 12  MTEVKLHGAWPSPFSYRVIWALALKGIPYQYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 71
           M EVKL G W SPFS RV WAL +KG+ Y+YIEED  NKS LLLQ NP+HKK+PVL+H G
Sbjct: 1   MAEVKLLGFWYSPFSRRVEWALKIKGVKYEYIEEDRDNKSSLLLQSNPIHKKVPVLIHNG 60

Query: 72  KPICESSIILQ 131
           K I ES +IL+
Sbjct: 61  KRIVESMVILE 71

Query: 132  191
           
Sbjct: 121  71

Query: 192  225
           
Sbjct: 181  71

[Close]

BLAST of WMU78668 vs. Swiss-Prot
Match: GSTU5_ARATH (Glutathione S-transferase U5 OS=Arabidopsis thaliana GN=GSTU5 PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 3.0e-20
Identity = 48/69 (69.57%), Postives = 52/69 (75.36%), Query Frame = 3


Query: 18  EVKLHGAWPSPFSYRVIWALALKGIPYQYIEEDLANKSPLLLQYNPVHKKIPVLVHAGKP 77
           EVKL G W SPFS RV  AL LKGIPY+Y+EE L NKSPLLL  NP+HKK+PVLVH GK 
Sbjct: 6   EVKLLGIWASPFSRRVEMALKLKGIPYEYVEEILENKSPLLLALNPIHKKVPVLVHNGKT 65

Query: 78  ICESSIILQ 137
           I ES +IL+
Sbjct: 66  ILESHVILE 74

Query: 138  197
           
Sbjct: 126  74

Query: 198  225
           
Sbjct: 186  74

[Close]

BLAST of WMU78668 vs. NCBI nr
Match: gi|659075966|ref|XP_008438427.1| (PREDICTED: probable glutathione S-transferase isoform X2 [Cucumis melo])

HSP 1 Score: 146.0 bits (367), Expect = 2.7e-32
Identity = 67/71 (94.37%), Postives = 69/71 (97.18%), Query Frame = 3


Query: 12  MTEVKLHGAWPSPFSYRVIWALALKGIPYQYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 71
           MTEVKLHGAWPSPFSYRVIWAL LKGIPY+YIEEDL+NKSPLLLQYNPVHKKIPVLVHAG
Sbjct: 1   MTEVKLHGAWPSPFSYRVIWALELKGIPYEYIEEDLSNKSPLLLQYNPVHKKIPVLVHAG 60

Query: 72  KPICESSIILQ 131
           KPICES IILQ
Sbjct: 61  KPICESMIILQ 71

Query: 132  191
           
Sbjct: 121  71

Query: 192  225
           
Sbjct: 181  71

[Close]

BLAST of WMU78668 vs. NCBI nr
Match: gi|778678021|ref|XP_011650902.1| (PREDICTED: probable glutathione S-transferase [Cucumis sativus])

HSP 1 Score: 140.6 bits (353), Expect = 1.1e-30
Identity = 64/71 (90.14%), Postives = 68/71 (95.77%), Query Frame = 3


Query: 12  MTEVKLHGAWPSPFSYRVIWALALKGIPYQYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 71
           MTEVKL+GAWPSPFSYRVIWALALKGIPY+Y+EEDL+NKS LLLQYNPVHKKIPV VHAG
Sbjct: 1   MTEVKLYGAWPSPFSYRVIWALALKGIPYEYVEEDLSNKSSLLLQYNPVHKKIPVFVHAG 60

Query: 72  KPICESSIILQ 131
           KPICES IILQ
Sbjct: 61  KPICESMIILQ 71

Query: 132  191
           
Sbjct: 121  71

Query: 192  225
           
Sbjct: 181  71

[Close]

BLAST of WMU78668 vs. NCBI nr
Match: gi|659075964|ref|XP_008438426.1| (PREDICTED: probable glutathione S-transferase isoform X1 [Cucumis melo])

HSP 1 Score: 139.4 bits (350), Expect = 2.5e-30
Identity = 65/71 (91.55%), Postives = 67/71 (94.37%), Query Frame = 3


Query: 12  MTEVKLHGAWPSPFSYRVIWALALKGIPYQYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 71
           MTEVKLHG WPSPFSYRVIWALALKGI Y+YIEEDL+NKSPLLLQYNPVHKKIPVLVHAG
Sbjct: 1   MTEVKLHGTWPSPFSYRVIWALALKGISYEYIEEDLSNKSPLLLQYNPVHKKIPVLVHAG 60

Query: 72  KPICESSIILQ 131
           KPI ES IILQ
Sbjct: 61  KPISESMIILQ 71

Query: 132  191
           
Sbjct: 121  71

Query: 192  225
           
Sbjct: 181  71

[Close]

BLAST of WMU78668 vs. NCBI nr
Match: gi|659075968|ref|XP_008438428.1| (PREDICTED: probable glutathione S-transferase isoform X3 [Cucumis melo])

HSP 1 Score: 139.4 bits (350), Expect = 2.5e-30
Identity = 65/71 (91.55%), Postives = 67/71 (94.37%), Query Frame = 3


Query: 12  MTEVKLHGAWPSPFSYRVIWALALKGIPYQYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 71
           MTEVKLHG WPSPFSYRVIWALALKGI Y+YIEEDL+NKSPLLLQYNPVHKKIPVLVHAG
Sbjct: 1   MTEVKLHGTWPSPFSYRVIWALALKGISYEYIEEDLSNKSPLLLQYNPVHKKIPVLVHAG 60

Query: 72  KPICESSIILQ 131
           KPI ES IILQ
Sbjct: 61  KPISESMIILQ 71

Query: 132  191
           
Sbjct: 121  71

Query: 192  225
           
Sbjct: 181  71

[Close]

BLAST of WMU78668 vs. NCBI nr
Match: gi|449433207|ref|XP_004134389.1| (PREDICTED: probable glutathione S-transferase [Cucumis sativus])

HSP 1 Score: 139.0 bits (349), Expect = 3.2e-30
Identity = 64/71 (90.14%), Postives = 67/71 (94.37%), Query Frame = 3


Query: 12  MTEVKLHGAWPSPFSYRVIWALALKGIPYQYIEEDLANKSPLLLQYNPVHKKIPVLVHAG 71
           M E+KLHG WPSPFSYRVIWALALKGIPYQYIEEDL+NKSPLLLQYNPVHKKIPVLVHAG
Sbjct: 1   MEELKLHGTWPSPFSYRVIWALALKGIPYQYIEEDLSNKSPLLLQYNPVHKKIPVLVHAG 60

Query: 72  KPICESSIILQ 131
           KPI ES IIL+
Sbjct: 61  KPISESMIILE 71

Query: 132  191
           
Sbjct: 121  71

Query: 192  225
           
Sbjct: 181  71

The following BLAST results are available for this feature:

BLAST of WMU78668 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G29450.1	1.7e-21	69.57	glutathione S-transferase tau 5	[more]
AT2G29440.1	5.0e-21	65.28	glutathione S-transferase tau 6	[more]
AT2G29420.1	2.5e-20	65.22	glutathione S-transferase tau 7	[more]
AT2G29490.1	3.2e-20	62.50	glutathione S-transferase TAU 1	[more]
AT5G62480.1	7.2e-20	63.77	glutathione S-transferase tau 9	[more]

BLAST of WMU78668 vs. Swiss-Prot


Match Name	E-value	Identity	Description
GSTX3_TOBAC	7.2e-22	69.01	Probable glutathione S-transferase OS=Nicotiana tabacum PE=2 SV=1	[more]
GSTX1_SOLTU	9.5e-22	69.01	Probable glutathione S-transferase OS=Solanum tuberosum GN=PRP1 PE=2 SV=1	[more]
GSTX2_TOBAC	2.1e-21	69.01	Probable glutathione S-transferase OS=Nicotiana tabacum PE=2 SV=1	[more]
GSTX1_TOBAC	2.3e-20	66.20	Probable glutathione S-transferase OS=Nicotiana tabacum PE=2 SV=1	[more]
GSTU5_ARATH	3.0e-20	69.57	Glutathione S-transferase U5 OS=Arabidopsis thaliana GN=GSTU5 PE=2 SV=1	[more]

BLAST of WMU78668 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659075966\|ref\|XP_008438427.1\|	2.7e-32	94.37	PREDICTED: probable glutathione S-transferase isoform X2 [Cucumis melo]	[more]
gi\|778678021\|ref\|XP_011650902.1\|	1.1e-30	90.14	PREDICTED: probable glutathione S-transferase [Cucumis sativus]	[more]
gi\|659075964\|ref\|XP_008438426.1\|	2.5e-30	91.55	PREDICTED: probable glutathione S-transferase isoform X1 [Cucumis melo]	[more]
gi\|659075968\|ref\|XP_008438428.1\|	2.5e-30	91.55	PREDICTED: probable glutathione S-transferase isoform X3 [Cucumis melo]	[more]
gi\|449433207\|ref\|XP_004134389.1\|	3.2e-30	90.14	PREDICTED: probable glutathione S-transferase [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR004045	Glutathione_S-Trfase_N
IPR012336	Thioredoxin-like_fold

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Cla021463	Cla021463	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
SSH0002296	SSH0002296	EST

InterPro

Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004045	Glutathione S-transferase, N-terminal	PFAM	PF02798	GST_N	coord: 8..75 score: 1.4
IPR004045	Glutathione S-transferase, N-terminal	PROFILE	PS50404	GST_NTER	coord: 6..76 score: 18
IPR012336	Thioredoxin-like fold	GENE3D	G3DSA:3.40.30.10		coord: 6..75 score: 6.4
IPR012336	Thioredoxin-like fold	unknown	SSF52833	Thioredoxin-like	coord: 5..75 score: 3.55
None	No IPR available	PANTHER	PTHR11260	GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING	coord: 5..74 score: 3.7
None	No IPR available	PANTHER	PTHR11260:SF227	SUBFAMILY NOT NAMED	coord: 5..74 score: 3.7