WMU75645 (transcribed_cluster) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	WMU75645
Type	transcribed_cluster
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	FAD/NAD(P)-binding oxidoreductase family protein
Location	Chr2 : 3841697 .. 3841919 (+)
Sequence length	225

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

GGGTTAGTATTGCAGGCGATGCGCTCCACTCGATGACCCCAGATCTCGGACAAGGAGGTTGTTCTGCATTGGAAGACGGTGTCATTCTTGCCCGATGTTTAGCCGGAGCTATGTGGAGGAATCCAAAGGAGGAAGTAGATGAGAAGGAGGAATATAAAAGAATTGAAAAGGGGTTGGAGAAATATGCAAAAGAGAGGAGATGGAGAAAGCATTAAGCTTATAACT

Library


Library category	ESTs
watermelon fruit 34d library	1

Homology

[Close]

BLAST of WMU75645 vs. TAIR10
Match: AT4G38540.1 (AT4G38540.1 FAD/NAD(P)-binding oxidoreductase family protein)

HSP 1 Score: 95.1 bits (235), Expect = 1.9e-20
Identity = 48/71 (67.61%), Postives = 56/71 (78.87%), Query Frame = 3


Query: 3   VSIAGDALHSMTPDLGQGGCSALEDGVILARCLAGAM-WRNPKEEVDEKEE--YKRIEKG 62
           V +AGDALH MTPD+GQGGCSA+EDGVILARCL  A+  ++ K E +E EE  YKRIE+G
Sbjct: 286 VCVAGDALHPMTPDIGQGGCSAMEDGVILARCLGEAIKAKSLKGETEENEEEGYKRIEEG 345

Query: 63  LEKYAKERRWR 122
           L+KYA ER+WR
Sbjct: 346 LKKYAGERKWR 356

Query: 123  182
           
Sbjct: 406  356

Query: 183  207
           
Sbjct: 466  356

[Close]

BLAST of WMU75645 vs. TAIR10
Match: AT5G05320.1 (AT5G05320.1 FAD/NAD(P)-binding oxidoreductase family protein)

HSP 1 Score: 95.1 bits (235), Expect = 1.9e-20
Identity = 48/70 (68.57%), Postives = 53/70 (75.71%), Query Frame = 3


Query: 3   VSIAGDALHSMTPDLGQGGCSALEDGVILARCLAGAM-WRNPKEEV-DEKEEYKRIEKGL 62
           V +AGDALH MTPD+GQGGCSA+EDGVILARCL  AM  +N K E  DE E Y+RIE GL
Sbjct: 285 VCVAGDALHPMTPDIGQGGCSAMEDGVILARCLGEAMKAKNMKGETEDENESYRRIEDGL 344

Query: 63  EKYAKERRWR 122
           +KYA  R+WR
Sbjct: 345 KKYAGSRKWR 354

Query: 123  182
           
Sbjct: 405  354

Query: 183  207
           
Sbjct: 465  354

[Close]

BLAST of WMU75645 vs. TAIR10
Match: AT4G15760.1 (AT4G15760.1 monooxygenase 1)

HSP 1 Score: 58.9 bits (141), Expect = 1.5e-09
Identity = 31/68 (45.59%), Postives = 43/68 (63.24%), Query Frame = 3


Query: 3   VSIAGDALHSMTPDLGQGGCSALEDGVILARCLAGAMWRNPKEEVDEKEEYKRIEKGLEK 62
           V++AGDA+H M P L QGG +ALED V+LARCLA  +  +  + + +    K IE+ +++
Sbjct: 307 VTVAGDAMHVMGPFLAQGGSAALEDAVVLARCLARKVGPDHGDLLKDC-SMKNIEEAIDE 366

Query: 63  YAKERRWR 122
           Y  ERR R
Sbjct: 367 YVDERRMR 373

Query: 123  182
           
Sbjct: 427  373

Query: 183  207
           
Sbjct: 487  373

[Close]

BLAST of WMU75645 vs. TAIR10
Match: AT2G35660.1 (AT2G35660.1 FAD/NAD(P)-binding oxidoreductase family protein)

HSP 1 Score: 50.8 bits (120), Expect = 4.1e-07
Identity = 29/65 (44.62%), Postives = 33/65 (50.77%), Query Frame = 3


Query: 3   VSIAGDALHSMTPDLGQGGCSALEDGVILARCLAGAMWRNPKEEVDEKEEYKRIEKGLEK 62
           V + GDA H MTP+LGQG C ALED V+LA  LA A+              + IE  +E 
Sbjct: 326 VVLVGDAWHPMTPNLGQGACCALEDSVVLANKLANAI----------NGGTESIEVAMES 380

Query: 63  YAKER 122
           Y  ER
Sbjct: 386 YGSER 380

Query: 123  182
           
Sbjct: 446  380

Query: 183  198
           
Sbjct: 506  380

[Close]

BLAST of WMU75645 vs. TAIR10
Match: AT2G29720.1 (AT2G29720.1 FAD/NAD(P)-binding oxidoreductase family protein)

HSP 1 Score: 49.7 bits (117), Expect = 9.1e-07
Identity = 28/56 (50.00%), Postives = 32/56 (57.14%), Query Frame = 3


Query: 3   VSIAGDALHSMTPDLGQGGCSALEDGVILARCLAGAMWRNPKEEVDEKEEYKRIEK 62
           V + GDA H MTP+LGQG C ALED V+LA  LA A+     E V+   E  R E+
Sbjct: 324 VVLVGDAWHPMTPNLGQGACCALEDSVLLANKLASAI-NGGTESVEGAMESYRSER 378

Query: 63   122
           
Sbjct: 384  378

Query: 123  171
           
Sbjct: 444  378

[Close]

BLAST of WMU75645 vs. Swiss-Prot
Match: ZEP_ONCHC (Zeaxanthin epoxidase, chloroplastic OS=Oncidium hybrid cultivar GN=ZEP PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 5.6e-06
Identity = 27/68 (39.71%), Postives = 40/68 (58.82%), Query Frame = 3


Query: 3   VSIAGDALHSMTPDLGQGGCSALEDGVILARCLAGAMWRNPKEEVDEKEEYKRIEKGLEK 62
           V++ GD++H+M P++GQGGC A+ED   LA  L  A     KE +  ++    ++  L++
Sbjct: 367 VTLLGDSVHAMQPNMGQGGCMAIEDSYQLAHELEKAR----KESIQSRKPMD-VKSALKR 426

Query: 63  YAKERRWR 122
           Y KERR R
Sbjct: 427 YEKERRLR 429

Query: 123  182
           
Sbjct: 487  429

Query: 183  207
           
Sbjct: 547  429

[Close]

BLAST of WMU75645 vs. Swiss-Prot
Match: HPXO_KLEP7 (FAD-dependent urate hydroxylase OS=Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) GN=hpxO PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 9.5e-06
Identity = 24/52 (46.15%), Postives = 33/52 (63.46%), Query Frame = 3


Query: 3   VSIAGDALHSMTPDLGQGGCSALEDGVILARCLAGAMWRNPKEEVDEKEEYK 62
           V++ GDA HS TPD+GQGGC+A+ED V+L     GA++R  ++      EY+
Sbjct: 280 VALLGDAGHSTTPDIGQGGCAAMEDAVVL-----GAVFRQTRDIAAALREYE 326

Query: 63   122
           
Sbjct: 340  326

Query: 123  159
           
Sbjct: 400  326

[Close]

BLAST of WMU75645 vs. NCBI nr
Match: gi|659094208|ref|XP_008447940.1| (PREDICTED: zeaxanthin epoxidase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 124.4 bits (311), Expect = 8.3e-26
Identity = 62/68 (91.18%), Postives = 64/68 (94.12%), Query Frame = 3


Query: 3   VSIAGDALHSMTPDLGQGGCSALEDGVILARCLAGAMWRNPKEEVDEKEEYKRIEKGLEK 62
           VSIAGDALHSMTPDLGQGGCSALEDGVILARCLA AM RNP  EV++KEEYKRIEKGLEK
Sbjct: 291 VSIAGDALHSMTPDLGQGGCSALEDGVILARCLAEAMSRNPNGEVEDKEEYKRIEKGLEK 350

Query: 63  YAKERRWR 122
           YAKERRWR
Sbjct: 351 YAKERRWR 358

Query: 123  182
           
Sbjct: 411  358

Query: 183  207
           
Sbjct: 471  358

[Close]

BLAST of WMU75645 vs. NCBI nr
Match: gi|449454223|ref|XP_004144855.1| (PREDICTED: zeaxanthin epoxidase, chloroplastic [Cucumis sativus])

HSP 1 Score: 121.3 bits (303), Expect = 7.0e-25
Identity = 59/68 (86.76%), Postives = 63/68 (92.65%), Query Frame = 3


Query: 3   VSIAGDALHSMTPDLGQGGCSALEDGVILARCLAGAMWRNPKEEVDEKEEYKRIEKGLEK 62
           ++IAGDALHSMTPDLGQGGCSALEDGVILARCL  AM RNP  EV++KEEYKRIEKGLEK
Sbjct: 292 ITIAGDALHSMTPDLGQGGCSALEDGVILARCLGEAMSRNPNGEVEDKEEYKRIEKGLEK 351

Query: 63  YAKERRWR 122
           YAKERRWR
Sbjct: 352 YAKERRWR 359

Query: 123  182
           
Sbjct: 412  359

Query: 183  207
           
Sbjct: 472  359

[Close]

BLAST of WMU75645 vs. NCBI nr
Match: gi|922408735|ref|XP_013470337.1| (FAD/NAD(P)-binding oxidoreductase family protein [Medicago truncatula])

HSP 1 Score: 111.7 bits (278), Expect = 5.5e-22
Identity = 53/68 (77.94%), Postives = 60/68 (88.24%), Query Frame = 3


Query: 3   VSIAGDALHSMTPDLGQGGCSALEDGVILARCLAGAMWRNPKEEVDEKEEYKRIEKGLEK 62
           V +AGDALH MTPDLGQGGCSALEDGV+LARCLA A  + PKEE +E+EEYKRIE+G +K
Sbjct: 680 VCVAGDALHPMTPDLGQGGCSALEDGVVLARCLAEAFSKKPKEEEEEEEEYKRIEEGFKK 739

Query: 63  YAKERRWR 122
           YAKERRWR
Sbjct: 740 YAKERRWR 747

Query: 123  182
           
Sbjct: 800  747

Query: 183  207
           
Sbjct: 860  747

[Close]

BLAST of WMU75645 vs. NCBI nr
Match: gi|590684201|ref|XP_007041787.1| (FAD/NAD(P)-binding oxidoreductase family protein, putative [Theobroma cacao])

HSP 1 Score: 104.4 bits (259), Expect = 8.9e-20
Identity = 51/72 (70.83%), Postives = 62/72 (86.11%), Query Frame = 3


Query: 3   VSIAGDALHSMTPDLGQGGCSALEDGVILARCLAGAMWRNP----KEEVDEKEEYKRIEK 62
           V +AGDALH MTPDLGQGGCSA+EDGV+LARCLA A+ + P    KE++DE+EEYKRIE 
Sbjct: 283 VCLAGDALHPMTPDLGQGGCSAMEDGVVLARCLAEALLKVPRGEIKEKIDEEEEYKRIEM 342

Query: 63  GLEKYAKERRWR 122
           GL+K+A+ERRWR
Sbjct: 343 GLKKFAQERRWR 354

Query: 123  182
           
Sbjct: 403  354

Query: 183  207
           
Sbjct: 463  354

[Close]

BLAST of WMU75645 vs. NCBI nr
Match: gi|720015221|ref|XP_010260765.1| (PREDICTED: zeaxanthin epoxidase, chloroplastic-like isoform X1 [Nelumbo nucifera])

HSP 1 Score: 102.4 bits (254), Expect = 3.4e-19
Identity = 49/71 (69.01%), Postives = 58/71 (81.69%), Query Frame = 3


Query: 3   VSIAGDALHSMTPDLGQGGCSALEDGVILARCLAGAMWRNPKEE---VDEKEEYKRIEKG 62
           V +AGDALH MTPD+GQGGCSALEDG++LA+CLA A+   PK E   +DEKEEY RI+  
Sbjct: 289 VCVAGDALHPMTPDIGQGGCSALEDGIVLAKCLAQALLMEPKREAKGIDEKEEYNRIKNA 348

Query: 63  LEKYAKERRWR 122
           LEK+AKERRWR
Sbjct: 349 LEKFAKERRWR 359

Query: 123  182
           
Sbjct: 409  359

Query: 183  207
           
Sbjct: 469  359

The following BLAST results are available for this feature:

BLAST of WMU75645 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G38540.1	1.9e-20	67.61	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT5G05320.1	1.9e-20	68.57	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT4G15760.1	1.5e-09	45.59	monooxygenase 1	[more]
AT2G35660.1	4.1e-07	44.62	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT2G29720.1	9.1e-07	50.00	FAD/NAD(P)-binding oxidoreductase family protein	[more]

BLAST of WMU75645 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ZEP_ONCHC	5.6e-06	39.71	Zeaxanthin epoxidase, chloroplastic OS=Oncidium hybrid cultivar GN=ZEP PE=2 SV=1	[more]
HPXO_KLEP7	9.5e-06	46.15	FAD-dependent urate hydroxylase OS=Klebsiella pneumoniae subsp. pneumoniae (stra...	[more]

BLAST of WMU75645 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659094208\|ref\|XP_008447940.1\|	8.3e-26	91.18	PREDICTED: zeaxanthin epoxidase, chloroplastic-like [Cucumis melo]	[more]
gi\|449454223\|ref\|XP_004144855.1\|	7.0e-25	86.76	PREDICTED: zeaxanthin epoxidase, chloroplastic [Cucumis sativus]	[more]
gi\|922408735\|ref\|XP_013470337.1\|	5.5e-22	77.94	FAD/NAD(P)-binding oxidoreductase family protein [Medicago truncatula]	[more]
gi\|590684201\|ref\|XP_007041787.1\|	8.9e-20	70.83	FAD/NAD(P)-binding oxidoreductase family protein, putative [Theobroma cacao]	[more]
gi\|720015221\|ref\|XP_010260765.1\|	3.4e-19	69.01	PREDICTED: zeaxanthin epoxidase, chloroplastic-like isoform X1 [Nelumbo nucifera...	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR002938	FAD-bd
IPR023753	FAD/NAD-binding_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0071949	FAD binding
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0016491	oxidoreductase activity

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Cla015815	Cla015815	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
S4_0049061	S4_0049061	EST

InterPro

Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002938	FAD-binding domain	PFAM	PF01494	FAD_binding_3	coord: 2..37 score: 6.
IPR023753	FAD/NAD(P)-binding domain	GENE3D	G3DSA:3.50.50.60		coord: 3..68 score: 5.
IPR023753	FAD/NAD(P)-binding domain	unknown	SSF51905	FAD/NAD(P)-binding domain	coord: 3..69 score: 7.13
None	No IPR available	PANTHER	PTHR13789	MONOOXYGENASE	coord: 2..69 score: 2.2
None	No IPR available	PANTHER	PTHR13789:SF185	FAD/NAD(P)-BINDING OXIDOREDUCTASE FAMILY PROTEIN	coord: 2..69 score: 2.2