WMU64448 (transcribed_cluster) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	WMU64448
Type	transcribed_cluster
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Unknown protein
Location	Chr7 : 13375920 .. 13402788 (-)
Sequence length	475

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

TGACCTCCACCAATCAATACCGCCCACTTATCCACCAAACGTAAACCTGGAGCAGTTTTTCTCGTTTCCAGAATGCGTGCTGGGCTTGCAGCATCTGCCATCGCCTTAGTTAAAGTTGCAATTCCACTCATTCTCTGCATAAAGTTCAGTGCCACCCTTTCAGCTACAAGGATGCTTTGTGCTTGCCCTGAAACTTTTCCAAATTGTAGTCCTTTATGAATATAATCTCCATCCTTTTGAGACCATTCCACCCTAATCGAAGGATCAACCTCCTGGAACACCATGTTTGCAAGAGCAATACCGGCAACAATGCCATCCTCTTTGGCCAAAAAGAAGCATCCACTAGCAATGTCAGAGGGGAATGGTAGCCAAAAGATGTTACATCACCAAAGTCAGCAGAATCTTCTGCAAGGGCTAACTGGATGATGCCACGCAGATCATAGGTAGGGTGAGATGGACGGCTGGATTGCTACTG

Library


Library category	ESTs
watermelon fruit 18d library	1

Homology

[Close]

BLAST of WMU64448 vs. TAIR10
Match: AT2G01350.1 (AT2G01350.1 quinolinate phoshoribosyltransferase)

HSP 1 Score: 193.0 bits (489), Expect = 1.4e-49
Identity = 94/119 (78.99%), Postives = 104/119 (87.39%), Query Frame = -3


Query: 3   PSDIASGCFFLAKEDGIVAGIALANMVFQEVDPSIRVEWSQKDGDYIHKGLQFGKVSGQA 62
           P D+    +FLAKEDGIVAG+ALA+M+F+ VDPS++VEW +KDGDY+HKGL+FGKVSG A
Sbjct: 72  PFDMEVEAYFLAKEDGIVAGVALADMIFEHVDPSLKVEWMRKDGDYVHKGLKFGKVSGNA 131

Query: 63  QSILVAERVALNFMQRMSGIATLTKAMADAASPARILETRKTAPGLRLVDKWAVLIGGG 122
             I+VAERV LNFMQRMSGIATLTK MADAASPA ILETRKTAPGLRLVDKWAVLIGGG
Sbjct: 132 HKIVVAERVLLNFMQRMSGIATLTKLMADAASPACILETRKTAPGLRLVDKWAVLIGGG 190

Query: 123  182
           
Sbjct: 192  190

Query: 183  242
           
Sbjct: 252  190

Query: 243  302
           
Sbjct: 312  190

Query: 303  360
           
Sbjct: 372  190

HSP 2 Score: 46.2 bits (108), Expect = 2.2e-05
Identity = 24/46 (52.17%), Postives = 28/46 (60.87%), Query Frame = -1


Query: 320 PSHPTYDLRGIIQLALAEDSADFGDVTSFGYHSPLTLLVDASFWPK 379
           PSHPTYDL+ +I+LALAED+   GDVT      P  + V+A F  K
Sbjct: 40  PSHPTYDLKAVIKLALAEDAGHTGDVTCMA-TIPFDMEVEAYFLAK 84

Query: 380  439
           
Sbjct: 100  84

Query: 440  458
           
Sbjct: 160  84

[Close]

BLAST of WMU64448 vs. Swiss-Prot
Match: NADC_ARATH (Nicotinate-nucleotide pyrophosphorylase [carboxylating], chloroplastic OS=Arabidopsis thaliana GN=QPT PE=2 SV=2)

HSP 1 Score: 193.0 bits (489), Expect = 2.5e-48
Identity = 94/119 (78.99%), Postives = 104/119 (87.39%), Query Frame = -3


Query: 3   PSDIASGCFFLAKEDGIVAGIALANMVFQEVDPSIRVEWSQKDGDYIHKGLQFGKVSGQA 62
           P D+    +FLAKEDGIVAG+ALA+M+F+ VDPS++VEW +KDGDY+HKGL+FGKVSG A
Sbjct: 72  PFDMEVEAYFLAKEDGIVAGVALADMIFEHVDPSLKVEWMRKDGDYVHKGLKFGKVSGNA 131

Query: 63  QSILVAERVALNFMQRMSGIATLTKAMADAASPARILETRKTAPGLRLVDKWAVLIGGG 122
             I+VAERV LNFMQRMSGIATLTK MADAASPA ILETRKTAPGLRLVDKWAVLIGGG
Sbjct: 132 HKIVVAERVLLNFMQRMSGIATLTKLMADAASPACILETRKTAPGLRLVDKWAVLIGGG 190

Query: 123  182
           
Sbjct: 192  190

Query: 183  242
           
Sbjct: 252  190

Query: 243  302
           
Sbjct: 312  190

Query: 303  360
           
Sbjct: 372  190

HSP 2 Score: 46.2 bits (108), Expect = 3.8e-04
Identity = 24/46 (52.17%), Postives = 28/46 (60.87%), Query Frame = -1


Query: 320 PSHPTYDLRGIIQLALAEDSADFGDVTSFGYHSPLTLLVDASFWPK 379
           PSHPTYDL+ +I+LALAED+   GDVT      P  + V+A F  K
Sbjct: 40  PSHPTYDLKAVIKLALAEDAGHTGDVTCMA-TIPFDMEVEAYFLAK 84

Query: 380  439
           
Sbjct: 100  84

Query: 440  458
           
Sbjct: 160  84

[Close]

BLAST of WMU64448 vs. Swiss-Prot
Match: NADC_ORYSJ (Nicotinate-nucleotide pyrophosphorylase [carboxylating], chloroplastic OS=Oryza sativa subsp. japonica GN=Os09g0553600 PE=2 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 1.1e-46
Identity = 89/119 (74.79%), Postives = 106/119 (89.08%), Query Frame = -3


Query: 3   PSDIASGCFFLAKEDGIVAGIALANMVFQEVDPSIRVEWSQKDGDYIHKGLQFGKVSGQA 62
           PSD+ +   F+AKEDG+VAGI+LA+M+F++VDPS++VEW + DG+Y+HKGLQFG+V G A
Sbjct: 95  PSDVKAEATFIAKEDGVVAGISLADMIFKQVDPSLKVEWFESDGNYVHKGLQFGRVYGCA 154

Query: 63  QSILVAERVALNFMQRMSGIATLTKAMADAASPARILETRKTAPGLRLVDKWAVLIGGG 122
           ++I+VAERV LNFMQRMSGIAT+TKAMADAA PA ILETRKTAPGLRLVDKWAVLIGGG
Sbjct: 155 RNIIVAERVVLNFMQRMSGIATMTKAMADAAHPACILETRKTAPGLRLVDKWAVLIGGG 213

Query: 123  182
           
Sbjct: 215  213

Query: 183  242
           
Sbjct: 275  213

Query: 243  302
           
Sbjct: 335  213

Query: 303  360
           
Sbjct: 395  213

HSP 2 Score: 42.7 bits (99), Expect = 4.2e-03
Identity = 17/27 (62.96%), Postives = 21/27 (77.78%), Query Frame = -1


Query: 377 PSHPTYDLRGIIQLALAEDSADFGDVT 436
           P+HPTYDL+ +I LAL+ED+ D GDV+
Sbjct: 63  PAHPTYDLKAVINLALSEDAGDRGDVS 89

Query: 437  458
           
Sbjct: 123  89

[Close]

BLAST of WMU64448 vs. Swiss-Prot
Match: NADC_BACSU (Probable nicotinate-nucleotide pyrophosphorylase [carboxylating] OS=Bacillus subtilis (strain 168) GN=nadC PE=1 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 2.3e-17
Identity = 47/111 (42.34%), Postives = 68/111 (61.26%), Query Frame = -3


Query: 3   LAKEDGIVAGIALANMVFQEVDPSIRVEWSQKDGDYIHKGLQFGKVSGQAQSILVAERVA 62
           +AK +GI AG A+    F  +D +++    +KDGD +HKG    ++ G A ++L  ERV 
Sbjct: 39  VAKSEGIFAGAAIIKEGFSLLDENVQSILHKKDGDMLHKGEVIAELHGPAAALLSGERVV 98

Query: 63  LNFMQRMSGIATLTKAMADAA--SPARILETRKTAPGLRLVDKWAVLIGGG 122
           LN +QR+SGIAT+T+           +I +TRKT PGLR+++K+AV  GGG
Sbjct: 99  LNLIQRLSGIATMTREAVRCLDDEQIKICDTRKTTPGLRMLEKYAVRAGGG 149

Query: 123  182
           
Sbjct: 159  149

Query: 183  242
           
Sbjct: 219  149

Query: 243  302
           
Sbjct: 279  149

Query: 303  330
           
Sbjct: 339  149

[Close]

BLAST of WMU64448 vs. Swiss-Prot
Match: NADC_PSEAE (Nicotinate-nucleotide pyrophosphorylase [carboxylating] OS=Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) GN=nadC PE=3 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 6.7e-17
Identity = 45/110 (40.91%), Postives = 66/110 (60.00%), Query Frame = -3


Query: 6   LAKEDGIVAGIALANMVFQEVDPSIRVEWSQKDGDYIHKGLQFGKVSGQAQSILVAERVA 65
           + +E   +AG A  + VF++VDP + V W  +DG  +       ++ G A+++L  ER A
Sbjct: 46  ITREHATIAGRAWVDEVFRQVDPRVLVTWQVEDGQRVEPNQMLFQLKGPARALLTGERSA 105

Query: 66  LNFMQRMSGIATLTKAMAD--AASPARILETRKTAPGLRLVDKWAVLIGG 125
           LNF+Q +SG AT ++  AD  A +  ++L+TRKT PGLRL  K+AV  GG
Sbjct: 106 LNFLQLLSGTATRSQHYADLVAGTAVKLLDTRKTLPGLRLAQKYAVTCGG 155

Query: 126  185
           
Sbjct: 166  155

Query: 186  245
           
Sbjct: 226  155

Query: 246  305
           
Sbjct: 286  155

Query: 306  330
           
Sbjct: 346  155

[Close]

BLAST of WMU64448 vs. Swiss-Prot
Match: NADC_RHORU (Probable nicotinate-nucleotide pyrophosphorylase [carboxylating] OS=Rhodospirillum rubrum GN=nadC PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.5e-16
Identity = 49/112 (43.75%), Postives = 68/112 (60.71%), Query Frame = -3


Query: 3   FLAKEDGIVAGIALANMVFQEVDPSIRVEWSQKDGDYIHKGLQFGKVSGQAQSILVAERV 62
           F+A++ GI+AG+  A   F  +D ++      +DG  I  G    +V+G A++IL AER 
Sbjct: 49  FVARQPGILAGLGCARSAFALLDDTVTFTTPLEDGAEIAAGQTVAEVAGAARTILAAERT 108

Query: 63  ALNFMQRMSGIATLTKAMADAA--SPARILETRKTAPGLRLVDKWAVLIGGG 122
           ALNF+  +SGIAT T+   DA   + AR+  TRKT PGLR ++K+AV  GGG
Sbjct: 109 ALNFLGHLSGIATRTRRFGDAIAHTRARLTCTRKTTPGLRGLEKYAVRCGGG 160

Query: 123  182
           
Sbjct: 169  160

Query: 183  242
           
Sbjct: 229  160

Query: 243  302
           
Sbjct: 289  160

Query: 303  333
           
Sbjct: 349  160

[Close]

BLAST of WMU64448 vs. NCBI nr
Match: gi|778730689|ref|XP_011659842.1| (PREDICTED: nicotinate-nucleotide pyrophosphorylase [carboxylating])

HSP 1 Score: 216.9 bits (551), Expect = 2.6e-53
Identity = 111/119 (93.28%), Postives = 114/119 (95.80%), Query Frame = -3


Query: 3   PSDIASGCFFLAKEDGIVAGIALANMVFQEVDPSIRVEWSQKDGDYIHKGLQFGKVSGQA 62
           PSD+    FFLAKEDGIVAGIALA+MVFQEVDPSIRVEWS+KDGDYIHKGLQFGKVSGQA
Sbjct: 101 PSDMLVDAFFLAKEDGIVAGIALADMVFQEVDPSIRVEWSKKDGDYIHKGLQFGKVSGQA 160

Query: 63  QSILVAERVALNFMQRMSGIATLTKAMADAASPARILETRKTAPGLRLVDKWAVLIGGG 122
           QSILVAERVALNFMQRMSGIATLTKAMADAASPA ILETRKTAPGLRLVDKWAVLIGGG
Sbjct: 161 QSILVAERVALNFMQRMSGIATLTKAMADAASPACILETRKTAPGLRLVDKWAVLIGGG 219

Query: 123  182
           
Sbjct: 221  219

Query: 183  242
           
Sbjct: 281  219

Query: 243  302
           
Sbjct: 341  219

Query: 303  360
           
Sbjct: 401  219

[Close]

BLAST of WMU64448 vs. NCBI nr
Match: gi|659109575|ref|XP_008454778.1| (PREDICTED: nicotinate-nucleotide pyrophosphorylase [carboxylating])

HSP 1 Score: 212.2 bits (539), Expect = 6.4e-52
Identity = 109/119 (91.60%), Postives = 113/119 (94.96%), Query Frame = -3


Query: 3   PSDIASGCFFLAKEDGIVAGIALANMVFQEVDPSIRVEWSQKDGDYIHKGLQFGKVSGQA 62
           PSD+    FFLAKEDGIVAGIALA+MVFQEVDPSIRVEWS+KDGDYI KGLQFGKVSG+A
Sbjct: 53  PSDMLVDAFFLAKEDGIVAGIALADMVFQEVDPSIRVEWSKKDGDYIRKGLQFGKVSGKA 112

Query: 63  QSILVAERVALNFMQRMSGIATLTKAMADAASPARILETRKTAPGLRLVDKWAVLIGGG 122
           QSILVAERVALNFMQRMSGIATLTKAMADAASPA ILETRKTAPGLRLVDKWAVLIGGG
Sbjct: 113 QSILVAERVALNFMQRMSGIATLTKAMADAASPACILETRKTAPGLRLVDKWAVLIGGG 171

Query: 123  182
           
Sbjct: 173  171

Query: 183  242
           
Sbjct: 233  171

Query: 243  302
           
Sbjct: 293  171

Query: 303  360
           
Sbjct: 353  171

[Close]

BLAST of WMU64448 vs. NCBI nr
Match: gi|659109569|ref|XP_008454775.1| (PREDICTED: nicotinate-nucleotide pyrophosphorylase [carboxylating])

HSP 1 Score: 212.2 bits (539), Expect = 6.4e-52
Identity = 109/119 (91.60%), Postives = 113/119 (94.96%), Query Frame = -3


Query: 3   PSDIASGCFFLAKEDGIVAGIALANMVFQEVDPSIRVEWSQKDGDYIHKGLQFGKVSGQA 62
           PSD+    FFLAKEDGIVAGIALA+MVFQEVDPSIRVEWS+KDGDYI KGLQFGKVSG+A
Sbjct: 101 PSDMLVDAFFLAKEDGIVAGIALADMVFQEVDPSIRVEWSKKDGDYIRKGLQFGKVSGKA 160

Query: 63  QSILVAERVALNFMQRMSGIATLTKAMADAASPARILETRKTAPGLRLVDKWAVLIGGG 122
           QSILVAERVALNFMQRMSGIATLTKAMADAASPA ILETRKTAPGLRLVDKWAVLIGGG
Sbjct: 161 QSILVAERVALNFMQRMSGIATLTKAMADAASPACILETRKTAPGLRLVDKWAVLIGGG 219

Query: 123  182
           
Sbjct: 221  219

Query: 183  242
           
Sbjct: 281  219

Query: 243  302
           
Sbjct: 341  219

Query: 303  360
           
Sbjct: 401  219

[Close]

BLAST of WMU64448 vs. NCBI nr
Match: gi|255570096|ref|XP_002526010.1| (PREDICTED: nicotinate-nucleotide pyrophosphorylase [carboxylating])

HSP 1 Score: 200.7 bits (509), Expect = 1.9e-48
Identity = 99/119 (83.19%), Postives = 110/119 (92.44%), Query Frame = -3


Query: 3   PSDIASGCFFLAKEDGIVAGIALANMVFQEVDPSIRVEWSQKDGDYIHKGLQFGKVSGQA 62
           PS++    +FLAKEDGI+AGIALA M+F EVDPS+ VEWS+KDGDY+HKGLQFGKVSG+A
Sbjct: 77  PSEMEVEAYFLAKEDGIIAGIALAEMLFHEVDPSLTVEWSRKDGDYVHKGLQFGKVSGRA 136

Query: 63  QSILVAERVALNFMQRMSGIATLTKAMADAASPARILETRKTAPGLRLVDKWAVLIGGG 122
            +I+VAERVALNFMQRMSGIATLTKAMADAASPA ILETRKTAPGLRLVDKWAVLIGGG
Sbjct: 137 HNIVVAERVALNFMQRMSGIATLTKAMADAASPACILETRKTAPGLRLVDKWAVLIGGG 195

Query: 123  182
           
Sbjct: 197  195

Query: 183  242
           
Sbjct: 257  195

Query: 243  302
           
Sbjct: 317  195

Query: 303  360
           
Sbjct: 377  195

[Close]

BLAST of WMU64448 vs. NCBI nr
Match: gi|1000952196|ref|XP_015578974.1| (PREDICTED: nicotinate-nucleotide pyrophosphorylase [carboxylating])

HSP 1 Score: 200.7 bits (509), Expect = 1.9e-48
Identity = 99/119 (83.19%), Postives = 110/119 (92.44%), Query Frame = -3


Query: 3   PSDIASGCFFLAKEDGIVAGIALANMVFQEVDPSIRVEWSQKDGDYIHKGLQFGKVSGQA 62
           PS++    +FLAKEDGI+AGIALA M+F EVDPS+ VEWS+KDGDY+HKGLQFGKVSG+A
Sbjct: 109 PSEMEVEAYFLAKEDGIIAGIALAEMLFHEVDPSLTVEWSRKDGDYVHKGLQFGKVSGRA 168

Query: 63  QSILVAERVALNFMQRMSGIATLTKAMADAASPARILETRKTAPGLRLVDKWAVLIGGG 122
            +I+VAERVALNFMQRMSGIATLTKAMADAASPA ILETRKTAPGLRLVDKWAVLIGGG
Sbjct: 169 HNIVVAERVALNFMQRMSGIATLTKAMADAASPACILETRKTAPGLRLVDKWAVLIGGG 227

Query: 123  182
           
Sbjct: 229  227

Query: 183  242
           
Sbjct: 289  227

Query: 243  302
           
Sbjct: 349  227

Query: 303  360
           
Sbjct: 409  227

The following BLAST results are available for this feature:

BLAST of WMU64448 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G01350.1	1.4e-49	78.99	quinolinate phoshoribosyltransferase	[more]

BLAST of WMU64448 vs. Swiss-Prot


Match Name	E-value	Identity	Description
NADC_ARATH	2.5e-48	78.99	Nicotinate-nucleotide pyrophosphorylase [carboxylating], chloroplastic OS=Arabid...	[more]
NADC_ORYSJ	1.1e-46	74.79	Nicotinate-nucleotide pyrophosphorylase [carboxylating], chloroplastic OS=Oryza ...	[more]
NADC_BACSU	2.3e-17	42.34	Probable nicotinate-nucleotide pyrophosphorylase [carboxylating] OS=Bacillus sub...	[more]
NADC_PSEAE	6.7e-17	40.91	Nicotinate-nucleotide pyrophosphorylase [carboxylating] OS=Pseudomonas aeruginos...	[more]
NADC_RHORU	1.5e-16	43.75	Probable nicotinate-nucleotide pyrophosphorylase [carboxylating] OS=Rhodospirill...	[more]

BLAST of WMU64448 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778730689\|ref\|XP_011659842.1\|	2.6e-53	93.28	PREDICTED: nicotinate-nucleotide pyrophosphorylase [carboxylating]	[more]
gi\|659109575\|ref\|XP_008454778.1\|	6.4e-52	91.60	PREDICTED: nicotinate-nucleotide pyrophosphorylase [carboxylating]	[more]
gi\|659109569\|ref\|XP_008454775.1\|	6.4e-52	91.60	PREDICTED: nicotinate-nucleotide pyrophosphorylase [carboxylating]	[more]
gi\|255570096\|ref\|XP_002526010.1\|	1.9e-48	83.19	PREDICTED: nicotinate-nucleotide pyrophosphorylase [carboxylating]	[more]
gi\|1000952196\|ref\|XP_015578974.1\|	1.9e-48	83.19	PREDICTED: nicotinate-nucleotide pyrophosphorylase [carboxylating]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR002638	Quinolinate_PRibosylTrfase_C
IPR013785	Aldolase_TIM
IPR022412	Quinolinate_PRibosylTrfase_N

Vocabulary: **Molecular Function**
Term	Definition
GO:0004514	nicotinate-nucleotide diphosphorylase (carboxylating) activity
GO:0003824	catalytic activity
GO:0016763	transferase activity, transferring pentosyl groups

Vocabulary: **Biological Process**
Term	Definition
GO:0009435	NAD biosynthetic process

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009435	NAD biosynthetic process
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0004514	nicotinate-nucleotide diphosphorylase (carboxylating) activity
molecular_function	GO:0016763	transferase activity, transferring pentosyl groups

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Cla001944	Cla001944	gene
Cla001945	Cla001945	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
S2_0109732	S2_0109732	EST

InterPro

Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002638	Quinolinate phosphoribosyl transferase, C-terminal	PFAM	PF01729	QRPTase_C	coord: 119..158 score: 4.9
IPR002638	Quinolinate phosphoribosyl transferase, C-terminal	unknown	SSF51690	Nicotinate/Quinolinate PRTase C-terminal domain-like	coord: 119..158 score: 7.85
IPR013785	Aldolase-type TIM barrel	GENE3D	G3DSA:3.20.20.70		coord: 138..158 score: 1.
IPR022412	Quinolinate phosphoribosyl transferase, N-terminal	GENE3D	G3DSA:3.90.1170.20		coord: 8..137 score: 1.2
IPR022412	Quinolinate phosphoribosyl transferase, N-terminal	PFAM	PF02749	QRPTase_N	coord: 29..117 score: 2.7
None	No IPR available	PANTHER	PTHR32179	FAMILY NOT NAMED	coord: 2..158 score: 3.0
None	No IPR available	unknown	SSF54675	Nicotinate/Quinolinate PRTase N-terminal domain-like	coord: 6..118 score: 3.06