WMU62482 (transcribed_cluster) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	WMU62482
Type	transcribed_cluster
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Cystathionine beta-lyase
Location	Chr10 : 1519119 .. 1519266 (+)
Sequence length	157

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

GGGGGCCATGAAAAGGGGCTGACGGTGGTGGTGGATAATACTTTTGCCCCCATGGTTCTGTCGCCGGCGAGACTCGGTGCTGACGTCGTCGTTCATAGTATTTCTAAGTTCATTAGCGGTGGAGCTGACATCATTGCAGGTAACTTTTTTCTTACAC

Library


Library category	ESTs
watermelon fruit 18d library	1

Homology

[Close]

BLAST of WMU62482 vs. TAIR10
Match: AT1G64660.1 (AT1G64660.1 methionine gamma-lyase)

HSP 1 Score: 88.2 bits (217), Expect = 1.6e-18
Identity = 43/45 (95.56%), Postives = 43/45 (95.56%), Query Frame = 1


Query: 7   HEKGLTVVVDNTFAPMVLSPARLGADVVVHSISKFISGGADIIAG 66
           HEKG+TVVVDNTFAPMVLSPA+LGADVVVHSISKFISGGADIIAG
Sbjct: 215 HEKGVTVVVDNTFAPMVLSPAKLGADVVVHSISKFISGGADIIAG 259

Query: 67   126
           
Sbjct: 275  259

Query: 127  142
           
Sbjct: 335  259

[Close]

BLAST of WMU62482 vs. TAIR10
Match: AT3G57050.1 (AT3G57050.1 cystathionine beta-lyase)

HSP 1 Score: 49.7 bits (117), Expect = 6.4e-07
Identity = 24/46 (52.17%), Postives = 32/46 (69.57%), Query Frame = 1


Query: 7   HEKGLTVVVDNTFAPMVLS-PARLGADVVVHSISKFISGGADIIAG 66
           H +G  V+VDN+    VLS P  LGAD+V+HS +KFI+G +D++AG
Sbjct: 244 HAQGALVLVDNSIMSPVLSRPLELGADIVMHSATKFIAGHSDVMAG 289

Query: 67   126
           
Sbjct: 304  289

Query: 127  142
           
Sbjct: 364  289

[Close]

BLAST of WMU62482 vs. TAIR10
Match: AT3G01120.1 (AT3G01120.1 Pyridoxal phosphate (PLP)-dependent transferases superfamily protein)

HSP 1 Score: 45.8 bits (107), Expect = 9.3e-06
Identity = 22/46 (47.83%), Postives = 29/46 (63.04%), Query Frame = 1


Query: 7   HEKGLTVVVDNTFA-PMVLSPARLGADVVVHSISKFISGGADIIAG 66
           H++G  V +D TFA P+      LGAD+VVHS +K+I G  D++AG
Sbjct: 345 HKRGTLVCIDGTFATPLNQKALALGADLVVHSATKYIGGHNDVLAG 390

Query: 67   126
           
Sbjct: 405  390

Query: 127  142
           
Sbjct: 465  390

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BLAST of WMU62482 vs. Swiss-Prot
Match: MGL_ARATH (Methionine gamma-lyase OS=Arabidopsis thaliana GN=MGL PE=1 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 2.9e-17
Identity = 43/45 (95.56%), Postives = 43/45 (95.56%), Query Frame = 1


Query: 7   HEKGLTVVVDNTFAPMVLSPARLGADVVVHSISKFISGGADIIAG 66
           HEKG+TVVVDNTFAPMVLSPA+LGADVVVHSISKFISGGADIIAG
Sbjct: 215 HEKGVTVVVDNTFAPMVLSPAKLGADVVVHSISKFISGGADIIAG 259

Query: 67   126
           
Sbjct: 275  259

Query: 127  142
           
Sbjct: 335  259

[Close]

BLAST of WMU62482 vs. Swiss-Prot
Match: MEGL_FUSNP (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum GN=mgl PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.0e-06
Identity = 24/40 (60.00%), Postives = 31/40 (77.50%), Query Frame = 1


Query: 25  VVVDNTFA-PMVLSPARLGADVVVHSISKFISGGADIIAG 84
           V+VDNTFA P +  P +LGAD+VVHS++K+I+G  D+IAG
Sbjct: 181 VIVDNTFATPYMQKPLKLGADIVVHSVTKYINGHGDVIAG 220

Query: 85   142
           
Sbjct: 241  220

[Close]

BLAST of WMU62482 vs. Swiss-Prot
Match: CGL_DICDI (Cystathionine gamma-lyase OS=Dictyostelium discoideum GN=cysA PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 1.8e-06
Identity = 23/46 (50.00%), Postives = 33/46 (71.74%), Query Frame = 1


Query: 7   HEKGLTVVVDNTF-APMVLSPARLGADVVVHSISKFISGGADIIAG 66
           H +G T+VVDNTF +P   +P  LGAD+V+HS++K+I+G +D + G
Sbjct: 168 HSRGATLVVDNTFMSPYFQNPLDLGADIVMHSVTKYINGHSDCVMG 213

Query: 67   126
           
Sbjct: 228  213

Query: 127  142
           
Sbjct: 288  213

[Close]

BLAST of WMU62482 vs. Swiss-Prot
Match: METB_MYCBO (Cystathionine gamma-synthase OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) GN=metB PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 3.0e-06
Identity = 24/47 (51.06%), Postives = 32/47 (68.09%), Query Frame = 1


Query: 4   GHEKGLTVVVDNTFA-PMVLSPARLGADVVVHSISKFISGGADIIAG 63
           G ++   V+VDNTFA P +  P RLGADVV+HS +K+I G +D++ G
Sbjct: 173 GTDRSAKVLVDNTFASPALQQPLRLGADVVLHSTTKYIGGHSDVVGG 219

Query: 64   123
           
Sbjct: 233  219

Query: 124  142
           
Sbjct: 293  219

[Close]

BLAST of WMU62482 vs. Swiss-Prot
Match: METB_MYCTO (Cystathionine gamma-synthase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) GN=metB PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 3.0e-06
Identity = 24/47 (51.06%), Postives = 32/47 (68.09%), Query Frame = 1


Query: 4   GHEKGLTVVVDNTFA-PMVLSPARLGADVVVHSISKFISGGADIIAG 63
           G ++   V+VDNTFA P +  P RLGADVV+HS +K+I G +D++ G
Sbjct: 173 GTDRSAKVLVDNTFASPALQQPLRLGADVVLHSTTKYIGGHSDVVGG 219

Query: 64   123
           
Sbjct: 233  219

Query: 124  142
           
Sbjct: 293  219

[Close]

BLAST of WMU62482 vs. NCBI nr
Match: gi|449456795|ref|XP_004146134.1| (PREDICTED: methionine gamma-lyase-like [Cucumis sativus])

HSP 1 Score: 91.3 bits (225), Expect = 5.5e-16
Identity = 44/46 (95.65%), Postives = 46/46 (100.00%), Query Frame = 1


Query: 4   GHEKGLTVVVDNTFAPMVLSPARLGADVVVHSISKFISGGADIIAG 63
           GHEKG+TVVVDNTFAPM+LSPARLGADVVVHSISKFISGGADIIAG
Sbjct: 213 GHEKGVTVVVDNTFAPMILSPARLGADVVVHSISKFISGGADIIAG 258

Query: 64   123
           
Sbjct: 273  258

Query: 124  142
           
Sbjct: 333  258

[Close]

BLAST of WMU62482 vs. NCBI nr
Match: gi|307136088|gb|ADN33936.1| (cystathionine gamma-synthase [Cucumis melo subsp. melo])

HSP 1 Score: 91.3 bits (225), Expect = 5.5e-16
Identity = 44/46 (95.65%), Postives = 46/46 (100.00%), Query Frame = 1


Query: 4   GHEKGLTVVVDNTFAPMVLSPARLGADVVVHSISKFISGGADIIAG 63
           GHEKG+TVVVDNTFAPM+LSPARLGADVVVHSISKFISGGADIIAG
Sbjct: 180 GHEKGVTVVVDNTFAPMILSPARLGADVVVHSISKFISGGADIIAG 225

Query: 64   123
           
Sbjct: 240  225

Query: 124  142
           
Sbjct: 300  225

[Close]

BLAST of WMU62482 vs. NCBI nr
Match: gi|659095405|ref|XP_008448562.1| (PREDICTED: methionine gamma-lyase-like [Cucumis melo])

HSP 1 Score: 91.3 bits (225), Expect = 5.5e-16
Identity = 44/46 (95.65%), Postives = 46/46 (100.00%), Query Frame = 1


Query: 4   GHEKGLTVVVDNTFAPMVLSPARLGADVVVHSISKFISGGADIIAG 63
           GHEKG+TVVVDNTFAPM+LSPARLGADVVVHSISKFISGGADIIAG
Sbjct: 212 GHEKGVTVVVDNTFAPMILSPARLGADVVVHSISKFISGGADIIAG 257

Query: 64   123
           
Sbjct: 272  257

Query: 124  142
           
Sbjct: 332  257

[Close]

BLAST of WMU62482 vs. NCBI nr
Match: gi|451811145|gb|AGF70153.1| (methionine gamma-lyase [Cucumis melo])

HSP 1 Score: 91.3 bits (225), Expect = 5.5e-16
Identity = 44/46 (95.65%), Postives = 46/46 (100.00%), Query Frame = 1


Query: 4   GHEKGLTVVVDNTFAPMVLSPARLGADVVVHSISKFISGGADIIAG 63
           GHEKG+TVVVDNTFAPM+LSPARLGADVVVHSISKFISGGADIIAG
Sbjct: 212 GHEKGVTVVVDNTFAPMILSPARLGADVVVHSISKFISGGADIIAG 257

Query: 64   123
           
Sbjct: 272  257

Query: 124  142
           
Sbjct: 332  257

[Close]

BLAST of WMU62482 vs. NCBI nr
Match: gi|727653358|ref|XP_010497416.1| (PREDICTED: methionine gamma-lyase-like [Camelina sativa])

HSP 1 Score: 90.5 bits (223), Expect = 9.3e-16
Identity = 44/46 (95.65%), Postives = 46/46 (100.00%), Query Frame = 1


Query: 7   HEKGLTVVVDNTFAPMVLSPARLGADVVVHSISKFISGGADIIAGN 66
           HEKG+TVVVDNTFAPMVLSPA+LGADVVVHSISKFISGGADIIAGN
Sbjct: 135 HEKGVTVVVDNTFAPMVLSPAKLGADVVVHSISKFISGGADIIAGN 180

Query: 67   126
           
Sbjct: 195  180

Query: 127  145
           
Sbjct: 255  180

The following BLAST results are available for this feature:

BLAST of WMU62482 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G64660.1	1.6e-18	95.56	methionine gamma-lyase	[more]
AT3G57050.1	6.4e-07	52.17	cystathionine beta-lyase	[more]
AT3G01120.1	9.3e-06	47.83	Pyridoxal phosphate (PLP)-dependent transferases superfamily protein	[more]

BLAST of WMU62482 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MGL_ARATH	2.9e-17	95.56	Methionine gamma-lyase OS=Arabidopsis thaliana GN=MGL PE=1 SV=1	[more]
MEGL_FUSNP	1.0e-06	60.00	L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum GN=mgl PE...	[more]
CGL_DICDI	1.8e-06	50.00	Cystathionine gamma-lyase OS=Dictyostelium discoideum GN=cysA PE=1 SV=1	[more]
METB_MYCBO	3.0e-06	51.06	Cystathionine gamma-synthase OS=Mycobacterium bovis (strain ATCC BAA-935 / AF212...	[more]
METB_MYCTO	3.0e-06	51.06	Cystathionine gamma-synthase OS=Mycobacterium tuberculosis (strain CDC 1551 / Os...	[more]

BLAST of WMU62482 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449456795\|ref\|XP_004146134.1\|	5.5e-16	95.65	PREDICTED: methionine gamma-lyase-like [Cucumis sativus]	[more]
gi\|307136088\|gb\|ADN33936.1\|	5.5e-16	95.65	cystathionine gamma-synthase [Cucumis melo subsp. melo]	[more]
gi\|659095405\|ref\|XP_008448562.1\|	5.5e-16	95.65	PREDICTED: methionine gamma-lyase-like [Cucumis melo]	[more]
gi\|451811145\|gb\|AGF70153.1\|	5.5e-16	95.65	methionine gamma-lyase [Cucumis melo]	[more]
gi\|727653358\|ref\|XP_010497416.1\|	9.3e-16	95.65	PREDICTED: methionine gamma-lyase-like [Camelina sativa]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR000277	Cys/Met-Metab_PyrdxlP-dep_enz
IPR015421	PyrdxlP-dep_Trfase_major
IPR015424	PyrdxlP-dep_Trfase

Vocabulary: **Molecular Function**
Term	Definition
GO:0030170	pyridoxal phosphate binding
GO:0003824	catalytic activity

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0030170	pyridoxal phosphate binding

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Cla008888	Cla008888	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
S2_0073520	S2_0073520	EST

InterPro

Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	PANTHER	PTHR11808	TRANS-SULFURATION ENZYME FAMILY MEMBER	coord: 3..47 score: 2.1
IPR000277	Cys/Met metabolism, pyridoxal phosphate-dependent enzyme	PFAM	PF01053	Cys_Met_Meta_PP	coord: 3..47 score: 2.9
IPR015421	Pyridoxal phosphate-dependent transferase, major region, subdomain 1	GENE3D	G3DSA:3.40.640.10		coord: 3..47 score: 2.1
IPR015424	Pyridoxal phosphate-dependent transferase	unknown	SSF53383	PLP-dependent transferases	coord: 3..47 score: 1.0
None	No IPR available	PANTHER	PTHR11808:SF55	SUBFAMILY NOT NAMED	coord: 3..47 score: 2.1