WMU58246 (transcribed_cluster) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	WMU58246
Type	transcribed_cluster
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	AMP-dependent synthetase and ligase family protein
Location	Chr1 : 22616115 .. 22616462 (+)
Sequence length	350

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

GGGGAATTGCTGCCGTAGGTGGAACAAATGTTTGTATTCGAAAATTCGATGCATCATTAATTTTTTTCCCTGATTCGTCGCCATCATGTGACACACTTATGTGGAGCTCCGGTCGTACTCAATATGCTAACGAACTCTCCTGACAATCGACCACTTGATCGACCGGTCAAGATTCTGACTGCCGGAGCACCACCGCCTGCGGCAGTTCTGCTTCGAATTGAATCATTGGGGTTTGTTGTCACTCATGGGTATGGATTAACTGAGAGTGGTGGGACGATCATCAGCTGTTCATGGAAGTCGGAGTGGAACCGGCTGACGGATGGTTAGGGTTCCGGCGATGGAAGAGAGAA

Library


Library category	ESTs
watermelon fruit 10d library	1

Homology

[Close]

BLAST of WMU58246 vs. TAIR10
Match: AT5G16370.1 (AT5G16370.1 acyl activating enzyme 5)

HSP 1 Score: 129.4 bits (324), Expect = 1.4e-30
Identity = 58/84 (69.05%), Postives = 70/84 (83.33%), Query Frame = 1


Query: 64  FSLIRRHHVTHLCGAPVVLNMLTNSPDNRPLDRPVKILTAGAPPPAAVLLRIESLGFVVT 123
           + LIR H VTH+CGAPVVLNML+ + + +PL+RPV ILTAGAPPPAAVLLR ES+GFV++
Sbjct: 266 YRLIRDHGVTHMCGAPVVLNMLSATNEFQPLNRPVNILTAGAPPPAAVLLRAESIGFVIS 325

Query: 124 HGYGLTESGGTIISCSWKSEWNRL 183
           HGYGLTE+ G  +SC+WK +WNRL
Sbjct: 326 HGYGLTETAGLNVSCAWKPQWNRL 349

Query: 184  243
           
Sbjct: 386  349

Query: 244  303
           
Sbjct: 446  349

Query: 304  316
           
Sbjct: 506  349

HSP 2 Score: 41.6 bits (96), Expect = 3.9e-04
Identity = 18/21 (85.71%), Postives = 18/21 (85.71%), Query Frame = 3


Query: 3   GIAAVGGTNVCIRKFDASLIF 62
           GIAAVGGTNVC+RKFDA LI+
Sbjct: 246 GIAAVGGTNVCLRKFDAPLIY 266

Query: 63   66
           
Sbjct: 306  266

[Close]

BLAST of WMU58246 vs. TAIR10
Match: AT1G21540.1 (AT1G21540.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 128.6 bits (322), Expect = 2.4e-30
Identity = 53/84 (63.10%), Postives = 68/84 (80.95%), Query Frame = 1


Query: 64  FSLIRRHHVTHLCGAPVVLNMLTNSPDNRPLDRPVKILTAGAPPPAAVLLRIESLGFVVT 123
           + LI +HHVTH+C AP+VLNMLTN P  +PL  PV+++TAGAPPPAA++ R E+LGF V 
Sbjct: 268 YDLIDKHHVTHMCAAPMVLNMLTNYPSRKPLKNPVQVMTAGAPPPAAIISRAETLGFNVG 327

Query: 124 HGYGLTESGGTIISCSWKSEWNRL 183
           HGYGLTE+GG ++SC+WK+EW+ L
Sbjct: 328 HGYGLTETGGPVVSCAWKAEWDHL 351

Query: 184  243
           
Sbjct: 388  351

Query: 244  303
           
Sbjct: 448  351

Query: 304  316
           
Sbjct: 508  351

[Close]

BLAST of WMU58246 vs. TAIR10
Match: AT5G16340.1 (AT5G16340.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 127.5 bits (319), Expect = 5.4e-30
Identity = 55/84 (65.48%), Postives = 69/84 (82.14%), Query Frame = 1


Query: 64  FSLIRRHHVTHLCGAPVVLNMLTNSPDNRPLDRPVKILTAGAPPPAAVLLRIESLGFVVT 123
           + LIR H VTH+CGAPVVLNML+ + +++PL+ PV ILTAG+PPPA VLLR ES+GFV++
Sbjct: 266 YRLIRDHGVTHMCGAPVVLNMLSATQESQPLNHPVNILTAGSPPPATVLLRAESIGFVIS 325

Query: 124 HGYGLTESGGTIISCSWKSEWNRL 183
           HGYGLTE+ G I+SC+WK +WN L
Sbjct: 326 HGYGLTETAGVIVSCAWKPKWNHL 349

Query: 184  243
           
Sbjct: 386  349

Query: 244  303
           
Sbjct: 446  349

Query: 304  316
           
Sbjct: 506  349

HSP 2 Score: 41.6 bits (96), Expect = 3.9e-04
Identity = 18/21 (85.71%), Postives = 18/21 (85.71%), Query Frame = 3


Query: 3   GIAAVGGTNVCIRKFDASLIF 62
           GIAAVGGTNVC+RKFDA LI+
Sbjct: 246 GIAAVGGTNVCLRKFDAPLIY 266

Query: 63   66
           
Sbjct: 306  266

[Close]

BLAST of WMU58246 vs. TAIR10
Match: AT1G77240.1 (AT1G77240.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 120.2 bits (300), Expect = 8.6e-28
Identity = 51/84 (60.71%), Postives = 67/84 (79.76%), Query Frame = 1


Query: 64  FSLIRRHHVTHLCGAPVVLNMLTNSPDNRPLDRPVKILTAGAPPPAAVLLRIESLGFVVT 123
           F+LI ++ VTH+C AP+VLNMLTN P  +PL  PVK++TAGAPPPA V+ + E+LGF V+
Sbjct: 266 FNLIDKYQVTHMCAAPMVLNMLTNHPAQKPLQSPVKVMTAGAPPPATVISKAEALGFDVS 325

Query: 124 HGYGLTESGGTIISCSWKSEWNRL 183
           HGYG+TE+GG ++SC+ K EW+RL
Sbjct: 326 HGYGMTETGGLVVSCALKPEWDRL 349

Query: 184  243
           
Sbjct: 386  349

Query: 244  303
           
Sbjct: 446  349

Query: 304  316
           
Sbjct: 506  349

[Close]

BLAST of WMU58246 vs. TAIR10
Match: AT1G21530.2 (AT1G21530.2 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 114.4 bits (285), Expect = 4.7e-26
Identity = 47/84 (55.95%), Postives = 65/84 (77.38%), Query Frame = 1


Query: 64  FSLIRRHHVTHLCGAPVVLNMLTNSPDNRPLDRPVKILTAGAPPPAAVLLRIESLGFVVT 123
           ++LI +H+VTH+C AP+VLNML N P + PL  PV+++T+GAPPPA ++ R ESLGF V+
Sbjct: 265 YNLIDKHNVTHMCAAPMVLNMLINYPLSTPLKNPVQVMTSGAPPPATIISRAESLGFNVS 324

Query: 124 HGYGLTESGGTIISCSWKSEWNRL 183
           H YGLTE+ G ++SC+WK +W+ L
Sbjct: 325 HSYGLTETSGPVVSCAWKPKWDHL 348

Query: 184  243
           
Sbjct: 385  348

Query: 244  303
           
Sbjct: 445  348

Query: 304  316
           
Sbjct: 505  348

[Close]

BLAST of WMU58246 vs. Swiss-Prot
Match: AAE5_ARATH (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana GN=AAE5 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 2.5e-29
Identity = 58/84 (69.05%), Postives = 70/84 (83.33%), Query Frame = 1


Query: 64  FSLIRRHHVTHLCGAPVVLNMLTNSPDNRPLDRPVKILTAGAPPPAAVLLRIESLGFVVT 123
           + LIR H VTH+CGAPVVLNML+ + + +PL+RPV ILTAGAPPPAAVLLR ES+GFV++
Sbjct: 266 YRLIRDHGVTHMCGAPVVLNMLSATNEFQPLNRPVNILTAGAPPPAAVLLRAESIGFVIS 325

Query: 124 HGYGLTESGGTIISCSWKSEWNRL 183
           HGYGLTE+ G  +SC+WK +WNRL
Sbjct: 326 HGYGLTETAGLNVSCAWKPQWNRL 349

Query: 184  243
           
Sbjct: 386  349

Query: 244  303
           
Sbjct: 446  349

Query: 304  316
           
Sbjct: 506  349

HSP 2 Score: 41.6 bits (96), Expect = 6.9e-03
Identity = 18/21 (85.71%), Postives = 18/21 (85.71%), Query Frame = 3


Query: 3   GIAAVGGTNVCIRKFDASLIF 62
           GIAAVGGTNVC+RKFDA LI+
Sbjct: 246 GIAAVGGTNVCLRKFDAPLIY 266

Query: 63   66
           
Sbjct: 306  266

[Close]

BLAST of WMU58246 vs. Swiss-Prot
Match: AEE9_ARATH (Probable acyl-activating enzyme 9 OS=Arabidopsis thaliana GN=AEE9 PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 4.3e-29
Identity = 53/84 (63.10%), Postives = 68/84 (80.95%), Query Frame = 1


Query: 64  FSLIRRHHVTHLCGAPVVLNMLTNSPDNRPLDRPVKILTAGAPPPAAVLLRIESLGFVVT 123
           + LI +HHVTH+C AP+VLNMLTN P  +PL  PV+++TAGAPPPAA++ R E+LGF V 
Sbjct: 268 YDLIDKHHVTHMCAAPMVLNMLTNYPSRKPLKNPVQVMTAGAPPPAAIISRAETLGFNVG 327

Query: 124 HGYGLTESGGTIISCSWKSEWNRL 183
           HGYGLTE+GG ++SC+WK+EW+ L
Sbjct: 328 HGYGLTETGGPVVSCAWKAEWDHL 351

Query: 184  243
           
Sbjct: 388  351

Query: 244  303
           
Sbjct: 448  351

Query: 304  316
           
Sbjct: 508  351

[Close]

BLAST of WMU58246 vs. Swiss-Prot
Match: AAE6_ARATH (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana GN=AAE6 PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 9.6e-29
Identity = 55/84 (65.48%), Postives = 69/84 (82.14%), Query Frame = 1


Query: 64  FSLIRRHHVTHLCGAPVVLNMLTNSPDNRPLDRPVKILTAGAPPPAAVLLRIESLGFVVT 123
           + LIR H VTH+CGAPVVLNML+ + +++PL+ PV ILTAG+PPPA VLLR ES+GFV++
Sbjct: 266 YRLIRDHGVTHMCGAPVVLNMLSATQESQPLNHPVNILTAGSPPPATVLLRAESIGFVIS 325

Query: 124 HGYGLTESGGTIISCSWKSEWNRL 183
           HGYGLTE+ G I+SC+WK +WN L
Sbjct: 326 HGYGLTETAGVIVSCAWKPKWNHL 349

Query: 184  243
           
Sbjct: 386  349

Query: 244  303
           
Sbjct: 446  349

Query: 304  316
           
Sbjct: 506  349

HSP 2 Score: 41.6 bits (96), Expect = 6.9e-03
Identity = 18/21 (85.71%), Postives = 18/21 (85.71%), Query Frame = 3


Query: 3   GIAAVGGTNVCIRKFDASLIF 62
           GIAAVGGTNVC+RKFDA LI+
Sbjct: 246 GIAAVGGTNVCLRKFDAPLIY 266

Query: 63   66
           
Sbjct: 306  266

[Close]

BLAST of WMU58246 vs. Swiss-Prot
Match: AAE4_ARATH (Probable acyl-activating enzyme 4 OS=Arabidopsis thaliana GN=AEE4 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 1.5e-26
Identity = 51/84 (60.71%), Postives = 67/84 (79.76%), Query Frame = 1


Query: 64  FSLIRRHHVTHLCGAPVVLNMLTNSPDNRPLDRPVKILTAGAPPPAAVLLRIESLGFVVT 123
           F+LI ++ VTH+C AP+VLNMLTN P  +PL  PVK++TAGAPPPA V+ + E+LGF V+
Sbjct: 266 FNLIDKYQVTHMCAAPMVLNMLTNHPAQKPLQSPVKVMTAGAPPPATVISKAEALGFDVS 325

Query: 124 HGYGLTESGGTIISCSWKSEWNRL 183
           HGYG+TE+GG ++SC+ K EW+RL
Sbjct: 326 HGYGMTETGGLVVSCALKPEWDRL 349

Query: 184  243
           
Sbjct: 386  349

Query: 244  303
           
Sbjct: 446  349

Query: 304  316
           
Sbjct: 506  349

[Close]

BLAST of WMU58246 vs. Swiss-Prot
Match: AEE10_ARATH (Probable acyl-activating enzyme 10 OS=Arabidopsis thaliana GN=AEE10 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 8.4e-25
Identity = 47/84 (55.95%), Postives = 65/84 (77.38%), Query Frame = 1


Query: 64  FSLIRRHHVTHLCGAPVVLNMLTNSPDNRPLDRPVKILTAGAPPPAAVLLRIESLGFVVT 123
           ++LI +H+VTH+C AP+VLNML N P + PL  PV+++T+GAPPPA ++ R ESLGF V+
Sbjct: 265 YNLIDKHNVTHMCAAPMVLNMLINYPLSTPLKNPVQVMTSGAPPPATIISRAESLGFNVS 324

Query: 124 HGYGLTESGGTIISCSWKSEWNRL 183
           H YGLTE+ G ++SC+WK +W+ L
Sbjct: 325 HSYGLTETSGPVVSCAWKPKWDHL 348

Query: 184  243
           
Sbjct: 385  348

Query: 244  303
           
Sbjct: 445  348

Query: 304  316
           
Sbjct: 505  348

[Close]

BLAST of WMU58246 vs. NCBI nr
Match: gi|449462358|ref|XP_004148908.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus])

HSP 1 Score: 166.4 bits (420), Expect = 3.0e-38
Identity = 78/84 (92.86%), Postives = 80/84 (95.24%), Query Frame = 1


Query: 64  FSLIRRHHVTHLCGAPVVLNMLTNSPDNRPLDRPVKILTAGAPPPAAVLLRIESLGFVVT 123
           FSLIRRHHVTHLCGAPVVLNMLTNSPDN PLDRPVKILTAGAPPPA+VLLR ESLGFVVT
Sbjct: 270 FSLIRRHHVTHLCGAPVVLNMLTNSPDNLPLDRPVKILTAGAPPPASVLLRTESLGFVVT 329

Query: 124 HGYGLTESGGTIISCSWKSEWNRL 183
           HGYGLTES GTII CSWKSEWN+L
Sbjct: 330 HGYGLTESAGTIICCSWKSEWNQL 353

Query: 184  243
           
Sbjct: 390  353

Query: 244  303
           
Sbjct: 450  353

Query: 304  316
           
Sbjct: 510  353

[Close]

BLAST of WMU58246 vs. NCBI nr
Match: gi|659126420|ref|XP_008463174.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo])

HSP 1 Score: 166.0 bits (419), Expect = 3.9e-38
Identity = 77/84 (91.67%), Postives = 80/84 (95.24%), Query Frame = 1


Query: 64  FSLIRRHHVTHLCGAPVVLNMLTNSPDNRPLDRPVKILTAGAPPPAAVLLRIESLGFVVT 123
           FSLIRRHHVTHLCGAPVVLNMLTNSPDN PLDRPVKILTAGAPPPA++LLR ESLGFVVT
Sbjct: 270 FSLIRRHHVTHLCGAPVVLNMLTNSPDNLPLDRPVKILTAGAPPPASILLRTESLGFVVT 329

Query: 124 HGYGLTESGGTIISCSWKSEWNRL 183
           HGYGLTES GTII CSWKSEWN+L
Sbjct: 330 HGYGLTESAGTIICCSWKSEWNQL 353

Query: 184  243
           
Sbjct: 390  353

Query: 244  303
           
Sbjct: 450  353

Query: 304  316
           
Sbjct: 510  353

[Close]

BLAST of WMU58246 vs. NCBI nr
Match: gi|449462360|ref|XP_004148909.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus])

HSP 1 Score: 155.6 bits (392), Expect = 5.3e-35
Identity = 71/84 (84.52%), Postives = 78/84 (92.86%), Query Frame = 1


Query: 64  FSLIRRHHVTHLCGAPVVLNMLTNSPDNRPLDRPVKILTAGAPPPAAVLLRIESLGFVVT 123
           FSLI RHHVTH+CGAPVVLNMLTNSP+NRPL+RPVKILTAGAPPPAAVLLR ESLGF V+
Sbjct: 289 FSLINRHHVTHMCGAPVVLNMLTNSPNNRPLERPVKILTAGAPPPAAVLLRTESLGFDVS 348

Query: 124 HGYGLTESGGTIISCSWKSEWNRL 183
           HGYGLTE+GG +I CSWK EWN+L
Sbjct: 349 HGYGLTETGGLVIFCSWKLEWNQL 372

Query: 184  243
           
Sbjct: 409  372

Query: 244  303
           
Sbjct: 469  372

Query: 304  316
           
Sbjct: 529  372

[Close]

BLAST of WMU58246 vs. NCBI nr
Match: gi|356553214|ref|XP_003544953.1| (PREDICTED: probable acyl-activating enzyme 6 [Glycine max])

HSP 1 Score: 155.2 bits (391), Expect = 6.9e-35
Identity = 66/84 (78.57%), Postives = 80/84 (95.24%), Query Frame = 1


Query: 64  FSLIRRHHVTHLCGAPVVLNMLTNSPDNRPLDRPVKILTAGAPPPAAVLLRIESLGFVVT 123
           +SLI+RHHVTH+CGAPVVLNMLTNSPDN+PL++PV+ILTAGAPPPAAVL R E+LGFVV+
Sbjct: 267 YSLIKRHHVTHMCGAPVVLNMLTNSPDNKPLEKPVQILTAGAPPPAAVLFRTEALGFVVS 326

Query: 124 HGYGLTESGGTIISCSWKSEWNRL 183
           HGYGLTE+GG ++SC+WK EWN+L
Sbjct: 327 HGYGLTETGGLVVSCAWKGEWNKL 350

Query: 184  243
           
Sbjct: 387  350

Query: 244  303
           
Sbjct: 447  350

Query: 304  316
           
Sbjct: 507  350

[Close]

BLAST of WMU58246 vs. NCBI nr
Match: gi|659126423|ref|XP_008463175.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo])

HSP 1 Score: 153.3 bits (386), Expect = 2.6e-34
Identity = 70/84 (83.33%), Postives = 77/84 (91.67%), Query Frame = 1


Query: 64  FSLIRRHHVTHLCGAPVVLNMLTNSPDNRPLDRPVKILTAGAPPPAAVLLRIESLGFVVT 123
           FSLI RHHVTHLCGAPVVLNMLTNSP+NRPL+RPVKILTAGAPPPAAVL R ESLGF V+
Sbjct: 268 FSLINRHHVTHLCGAPVVLNMLTNSPNNRPLERPVKILTAGAPPPAAVLFRTESLGFDVS 327

Query: 124 HGYGLTESGGTIISCSWKSEWNRL 183
           HGYGLTE+GG +I CSWK +WN+L
Sbjct: 328 HGYGLTETGGLVIFCSWKLKWNQL 351

Query: 184  243
           
Sbjct: 388  351

Query: 244  303
           
Sbjct: 448  351

Query: 304  316
           
Sbjct: 508  351

The following BLAST results are available for this feature:

BLAST of WMU58246 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G16370.1	1.4e-30	69.05	acyl activating enzyme 5	[more]
AT1G21540.1	2.4e-30	63.10	AMP-dependent synthetase and ligase family protein	[more]
AT5G16340.1	5.4e-30	65.48	AMP-dependent synthetase and ligase family protein	[more]
AT1G77240.1	8.6e-28	60.71	AMP-dependent synthetase and ligase family protein	[more]
AT1G21530.2	4.7e-26	55.95	AMP-dependent synthetase and ligase family protein	[more]

BLAST of WMU58246 vs. Swiss-Prot


Match Name	E-value	Identity	Description
AAE5_ARATH	2.5e-29	69.05	Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana GN=AAE5 P...	[more]
AEE9_ARATH	4.3e-29	63.10	Probable acyl-activating enzyme 9 OS=Arabidopsis thaliana GN=AEE9 PE=2 SV=1	[more]
AAE6_ARATH	9.6e-29	65.48	Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana GN=AAE6 PE=2 SV=1	[more]
AAE4_ARATH	1.5e-26	60.71	Probable acyl-activating enzyme 4 OS=Arabidopsis thaliana GN=AEE4 PE=2 SV=1	[more]
AEE10_ARATH	8.4e-25	55.95	Probable acyl-activating enzyme 10 OS=Arabidopsis thaliana GN=AEE10 PE=2 SV=1	[more]

BLAST of WMU58246 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449462358\|ref\|XP_004148908.1\|	3.0e-38	92.86	PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus]	[more]
gi\|659126420\|ref\|XP_008463174.1\|	3.9e-38	91.67	PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo]	[more]
gi\|449462360\|ref\|XP_004148909.1\|	5.3e-35	84.52	PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus]	[more]
gi\|356553214\|ref\|XP_003544953.1\|	6.9e-35	78.57	PREDICTED: probable acyl-activating enzyme 6 [Glycine max]	[more]
gi\|659126423\|ref\|XP_008463175.1\|	2.6e-34	83.33	PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Cla009110	Cla009110	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
S1_0157609	S1_0157609	EST

InterPro

Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	G3DSA:3.40.50.980		coord: 4..92 score: 1.
None	No IPR available	PANTHER	PTHR24095	FAMILY NOT NAMED	coord: 2..91 score: 5.4
None	No IPR available	PANTHER	PTHR24095:SF177	ACYL-ACTIVATING ENZYME 5, PEROXISOMAL-RELATED	coord: 2..91 score: 5.4
None	No IPR available	unknown	SSF56801	Acetyl-CoA synthetase-like	coord: 5..92 score: 9.4