WMU54043 (transcribed_cluster) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	WMU54043
Type	transcribed_cluster
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Pectinesterase
Location	Chr5 : 2209811 .. 2209982 (+)
Sequence length	179

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

CAATGCGACATTTACGGCACCGTCGATTTCATCTTCGGCAACGCCGCCGTCGTAATCCAAGATTCCAACATCATCGCTCGTGATCCTCCGAACAAAACCATCACACTCACCGCCCAAGGCCGCCACCCGAGGGTCCGTAAGGTCAGAACACCGGAATCTCCATCCACAATTGCAGAGTC

Library


Library category	ESTs
watermelon fruit 10d library	1

Homology

[Close]

BLAST of WMU54043 vs. TAIR10
Match: AT2G45220.1 (AT2G45220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 64.3 bits (155), Expect = 2.9e-11
Identity = 29/40 (72.50%), Postives = 32/40 (80.00%), Query Frame = 1


Query: 4   CDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGR 63
           CD+YGTVDFIFGNAA V+Q+ NI AR P +KT T+TAQGR
Sbjct: 344 CDVYGTVDFIFGNAAAVLQNCNIFARRPRSKTNTITAQGR 383

Query: 64   123
           
Sbjct: 404  383

[Close]

BLAST of WMU54043 vs. TAIR10
Match: AT4G00190.1 (AT4G00190.1 pectin methylesterase 38)

HSP 1 Score: 62.8 bits (151), Expect = 8.3e-11
Identity = 28/41 (68.29%), Postives = 31/41 (75.61%), Query Frame = 1


Query: 1   QCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGR 60
           +CDIYGTVDFIFGNAA V+Q+  I AR+PPN   T+TAQ R
Sbjct: 307 ECDIYGTVDFIFGNAAAVLQNCRIFARNPPNGVNTITAQSR 347

Query: 61   120
           
Sbjct: 367  347

Query: 121  124
           
Sbjct: 427  347

[Close]

BLAST of WMU54043 vs. TAIR10
Match: AT1G02810.1 (AT1G02810.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 57.8 bits (138), Expect = 2.7e-09
Identity = 27/42 (64.29%), Postives = 31/42 (73.81%), Query Frame = 1


Query: 1   QCDIYGTVDFIFGNAAVVIQDSNIIARDP-PNKTITLTAQGR 60
           +CD+YGTVDFIFGNAAVV Q+ N+  R P PN+   +TAQGR
Sbjct: 408 ECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGR 449

Query: 61   120
           
Sbjct: 468  449

Query: 121  124
           
Sbjct: 528  449

[Close]

BLAST of WMU54043 vs. TAIR10
Match: AT4G02330.1 (AT4G02330.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 57.4 bits (137), Expect = 3.5e-09
Identity = 28/42 (66.67%), Postives = 30/42 (71.43%), Query Frame = 1


Query: 1   QCDIYGTVDFIFGNAAVVIQDSNIIARDP-PNKTITLTAQGR 60
           +CDIYGTVDFIFGNAAVV QD N+  R P  N+   +TAQGR
Sbjct: 402 ECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAITAQGR 443

Query: 61   120
           
Sbjct: 462  443

Query: 121  124
           
Sbjct: 522  443

[Close]

BLAST of WMU54043 vs. TAIR10
Match: AT4G02300.1 (AT4G02300.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 57.0 bits (136), Expect = 4.6e-09
Identity = 27/43 (62.79%), Postives = 31/43 (72.09%), Query Frame = 1


Query: 1   QCDIYGTVDFIFGNAAVVIQDSNIIARDP-PNKTITLTAQGRH 60
           +CDIYGT+DFIFGNAAVV Q+S++ AR P P   I  TAQ R+
Sbjct: 362 ECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRN 404

Query: 61   120
           
Sbjct: 422  404

Query: 121  127
           
Sbjct: 482  404

[Close]

BLAST of WMU54043 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.9e-12
Identity = 33/41 (80.49%), Postives = 35/41 (85.37%), Query Frame = 1


Query: 1   QCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGR 60
           +CDIYGTVDFIFGNAAVV+Q+ NI AR PPN+T TLTAQGR
Sbjct: 341 ECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTAQGR 381

Query: 61   120
           
Sbjct: 401  381

Query: 121  124
           
Sbjct: 461  381

[Close]

BLAST of WMU54043 vs. Swiss-Prot
Match: PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=PME17 PE=2 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 5.1e-10
Identity = 29/40 (72.50%), Postives = 32/40 (80.00%), Query Frame = 1


Query: 4   CDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGR 63
           CD+YGTVDFIFGNAA V+Q+ NI AR P +KT T+TAQGR
Sbjct: 344 CDVYGTVDFIFGNAAAVLQNCNIFARRPRSKTNTITAQGR 383

Query: 64   123
           
Sbjct: 404  383

[Close]

BLAST of WMU54043 vs. Swiss-Prot
Match: PME38_ARATH (Putative pectinesterase/pectinesterase inhibitor 38 OS=Arabidopsis thaliana GN=PME38 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 1.5e-09
Identity = 28/41 (68.29%), Postives = 31/41 (75.61%), Query Frame = 1


Query: 1   QCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGR 60
           +CDIYGTVDFIFGNAA V+Q+  I AR+PPN   T+TAQ R
Sbjct: 307 ECDIYGTVDFIFGNAAAVLQNCRIFARNPPNGVNTITAQSR 347

Query: 61   120
           
Sbjct: 367  347

Query: 121  124
           
Sbjct: 427  347

[Close]

BLAST of WMU54043 vs. Swiss-Prot
Match: PME7_ARATH (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana GN=PME7 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 4.7e-08
Identity = 27/42 (64.29%), Postives = 31/42 (73.81%), Query Frame = 1


Query: 1   QCDIYGTVDFIFGNAAVVIQDSNIIARDP-PNKTITLTAQGR 60
           +CD+YGTVDFIFGNAAVV Q+ N+  R P PN+   +TAQGR
Sbjct: 408 ECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGR 449

Query: 61   120
           
Sbjct: 468  449

Query: 121  124
           
Sbjct: 528  449

[Close]

BLAST of WMU54043 vs. Swiss-Prot
Match: PME41_ARATH (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana GN=PME41 PE=2 SV=2)

HSP 1 Score: 57.4 bits (137), Expect = 6.2e-08
Identity = 28/42 (66.67%), Postives = 30/42 (71.43%), Query Frame = 1


Query: 1   QCDIYGTVDFIFGNAAVVIQDSNIIARDP-PNKTITLTAQGR 60
           +CDIYGTVDFIFGNAAVV QD N+  R P  N+   +TAQGR
Sbjct: 402 ECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAITAQGR 443

Query: 61   120
           
Sbjct: 462  443

Query: 121  124
           
Sbjct: 522  443

[Close]

BLAST of WMU54043 vs. NCBI nr
Match: gi|449432283|ref|XP_004133929.1| (PREDICTED: pectinesterase 2 [Cucumis sativus])

HSP 1 Score: 83.6 bits (205), Expect = 1.3e-13
Identity = 39/41 (95.12%), Postives = 40/41 (97.56%), Query Frame = 1


Query: 1   QCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGR 60
           QCDIYGTVDFIFGNAAVV+QD NIIARDPPNKTITLTAQGR
Sbjct: 344 QCDIYGTVDFIFGNAAVVLQDCNIIARDPPNKTITLTAQGR 384

Query: 61   120
           
Sbjct: 404  384

Query: 121  124
           
Sbjct: 464  384

[Close]

BLAST of WMU54043 vs. NCBI nr
Match: gi|659075527|ref|XP_008438193.1| (PREDICTED: pectinesterase 2 [Cucumis melo])

HSP 1 Score: 82.4 bits (202), Expect = 2.9e-13
Identity = 38/41 (92.68%), Postives = 40/41 (97.56%), Query Frame = 1


Query: 1   QCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGR 60
           QCDIYGTVDFIFGNAAVV+QD NIIARDPPNKTIT+TAQGR
Sbjct: 346 QCDIYGTVDFIFGNAAVVLQDCNIIARDPPNKTITVTAQGR 386

Query: 61   120
           
Sbjct: 406  386

Query: 121  124
           
Sbjct: 466  386

[Close]

BLAST of WMU54043 vs. NCBI nr
Match: gi|976903303|gb|KVH91211.1| (Pectinesterase, active site-containing protein [Cynara cardunculus var. scolymus])

HSP 1 Score: 76.6 bits (187), Expect = 1.6e-11
Identity = 36/41 (87.80%), Postives = 37/41 (90.24%), Query Frame = 1


Query: 1   QCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGR 60
           QCDIYGTVDFIFGNAAVV+Q  NI ARDPPNKT TLTAQGR
Sbjct: 342 QCDIYGTVDFIFGNAAVVLQSCNIYARDPPNKTNTLTAQGR 382

Query: 61   120
           
Sbjct: 402  382

Query: 121  124
           
Sbjct: 462  382

[Close]

BLAST of WMU54043 vs. NCBI nr
Match: gi|703108167|ref|XP_010098938.1| (Pectinesterase 2 [Morus notabilis])

HSP 1 Score: 75.1 bits (183), Expect = 4.6e-11
Identity = 34/41 (82.93%), Postives = 38/41 (92.68%), Query Frame = 1


Query: 1   QCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGR 60
           +CDIYGTVDFIFGNAAVV+Q+ NI ARDPPNKT T+TAQGR
Sbjct: 353 ECDIYGTVDFIFGNAAVVLQNCNIYARDPPNKTNTITAQGR 393

Query: 61   120
           
Sbjct: 413  393

Query: 121  124
           
Sbjct: 473  393

[Close]

BLAST of WMU54043 vs. NCBI nr
Match: gi|697149003|ref|XP_009628696.1| (PREDICTED: pectinesterase 2 [Nicotiana tomentosiformis])

HSP 1 Score: 75.1 bits (183), Expect = 4.6e-11
Identity = 34/41 (82.93%), Postives = 38/41 (92.68%), Query Frame = 1


Query: 1   QCDIYGTVDFIFGNAAVVIQDSNIIARDPPNKTITLTAQGR 60
           +CDIYGTVDFIFGNAAVV+Q+ NI ARDPPNKT T+TAQGR
Sbjct: 380 ECDIYGTVDFIFGNAAVVLQNCNIFARDPPNKTNTVTAQGR 420

Query: 61   120
           
Sbjct: 440  420

Query: 121  124
           
Sbjct: 500  420

The following BLAST results are available for this feature:

BLAST of WMU54043 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G45220.1	2.9e-11	72.50	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G00190.1	8.3e-11	68.29	pectin methylesterase 38	[more]
AT1G02810.1	2.7e-09	64.29	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02330.1	3.5e-09	66.67	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02300.1	4.6e-09	62.79	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

BLAST of WMU54043 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PME2_CITSI	1.9e-12	80.49	Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1	[more]
PME17_ARATH	5.1e-10	72.50	Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=P...	[more]
PME38_ARATH	1.5e-09	68.29	Putative pectinesterase/pectinesterase inhibitor 38 OS=Arabidopsis thaliana GN=P...	[more]
PME7_ARATH	4.7e-08	64.29	Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana GN=PM...	[more]
PME41_ARATH	6.2e-08	66.67	Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana GN=P...	[more]

BLAST of WMU54043 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449432283\|ref\|XP_004133929.1\|	1.3e-13	95.12	PREDICTED: pectinesterase 2 [Cucumis sativus]	[more]
gi\|659075527\|ref\|XP_008438193.1\|	2.9e-13	92.68	PREDICTED: pectinesterase 2 [Cucumis melo]	[more]
gi\|976903303\|gb\|KVH91211.1\|	1.6e-11	87.80	Pectinesterase, active site-containing protein [Cynara cardunculus var. scolymus...	[more]
gi\|703108167\|ref\|XP_010098938.1\|	4.6e-11	82.93	Pectinesterase 2 [Morus notabilis]	[more]
gi\|697149003\|ref\|XP_009628696.1\|	4.6e-11	82.93	PREDICTED: pectinesterase 2 [Nicotiana tomentosiformis]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR000070	Pectinesterase_cat
IPR011050	Pectin_lyase_fold/virulence
IPR012334	Pectin_lyas_fold

Vocabulary: **Cellular Component**
Term	Definition
GO:0005618	cell wall

Vocabulary: **Molecular Function**
Term	Definition
GO:0030599	pectinesterase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0042545	cell wall modification

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
cellular_component	GO:0005618	cell wall
molecular_function	GO:0030599	pectinesterase activity

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Cla021325	Cla021325	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
S1_0095172	S1_0095172	EST

InterPro

Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 1..43 score: 6.9
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 2..42 score: 1.3
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 1..43 score: 4.2
None	No IPR available	PANTHER	PTHR31707	FAMILY NOT NAMED	coord: 1..41 score: 9.9
None	No IPR available	PANTHER	PTHR31707:SF11	PECTINESTERASE/PECTINESTERASE INHIBITOR 17-RELATED	coord: 1..41 score: 9.9