WMU53301 (transcribed_cluster) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	WMU53301
Type	transcribed_cluster
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Pentatricopeptide repeat-containing protein
Location	Chr3 : 8652511 .. 8652885 (-)
Sequence length	377

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

TTAGTTTCTGCAAACCAGTTCAGGTTAGCAGAAACACTTCTTGATAGGATGAAGGAGGAGAAAATTGATGTCACTGAGGATATACTTCTCTCCATTTGTAGGGCTTATGGTCGTATCCATAAGCCATTGGATTCCATAAGAGTTTTCCATAAAATGCAGGATTTTCCATTGCAAGCCTACAGAGAAGTCTTACATTTCAGTGCTTGCCATTCTTGTGGAAGAGAATCAATTAAGATTAGCTTTTAGATTTTATAGGTATATGAGAAAAGTAGGTATTCCCCCTACTGTAGCTTCTCTTAATGTTCTAATCAAAGCCTTTTGTAAGAATAGTGTAACCATGGAAAAAGCAATACACATTTTTCGTGAAATGTCTAACT

Library


Library category	ESTs
watermelon fruit 10d library	1

Homology

[Close]

BLAST of WMU53301 vs. TAIR10
Match: AT5G46100.1 (AT5G46100.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 99.0 bits (245), Expect = 2.2e-21
Identity = 45/73 (61.64%), Postives = 59/73 (80.82%), Query Frame = 2


Query: 152 KCRIFHCKPTEKSYISVLAILVEENQLRLAFRFYRYMRKVGIPPTVASLNVLIKAFCKNS 211
           K + F C P++K+Y++VLAILVEENQL LAF+FY+ MR++G+PPTVASLNVLIKA C+N 
Sbjct: 111 KMKDFDCDPSQKAYVTVLAILVEENQLNLAFKFYKNMREIGLPPTVASLNVLIKALCRND 170

Query: 212 VTMEKAIHIFREM 271
            T++  + IF EM
Sbjct: 171 GTVDAGLKIFLEM 183

Query: 272  331
           
Sbjct: 231  183

Query: 332  371
           
Sbjct: 291  183

HSP 2 Score: 81.6 bits (200), Expect = 3.7e-16
Identity = 38/55 (69.09%), Postives = 44/55 (80.00%), Query Frame = 1


Query: 1   LVSANQFRLAETLLDRMKEEKIDVTEDILLSICRAYGRIHKPLDSIRVFHKMQDF 60
           LVSAN+F+ AE L+ RMK E   V+EDILLSICR YGR+H+P DS+RVFHKM+DF
Sbjct: 61  LVSANKFKAAEDLIVRMKIENCVVSEDILLSICRGYGRVHRPFDSLRVFHKMKDF 115

Query: 61   120
           
Sbjct: 121  115

Query: 121  166
           
Sbjct: 181  115

[Close]

BLAST of WMU53301 vs. TAIR10
Match: AT3G48810.1 (AT3G48810.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 55.8 bits (133), Expect = 2.2e-08
Identity = 30/79 (37.97%), Postives = 46/79 (58.23%), Query Frame = 2


Query: 140 EFSIKCRIFHCKPTEKSYISVLAILVEENQLRLAFRFYRYMRKVGIPPTVASLNVLIKAF 199
           E   + + F C P+ K Y  VL  L+ EN++++ +  YR M++ G  P V + NVL+KA 
Sbjct: 132 EMFYRIKEFGCDPSVKIYNHVLDTLLGENRIQMIYMVYRDMKRDGFEPNVFTYNVLLKAL 191

Query: 200 CKNSVTMEKAIHIFREMSN 259
           CKN+  ++ A  +  EMSN
Sbjct: 192 CKNN-KVDGAKKLLVEMSN 209

Query: 260  319
           
Sbjct: 252  209

Query: 320  377
           
Sbjct: 312  209

HSP 2 Score: 34.7 bits (78), Expect = 5.1e-02
Identity = 19/65 (29.23%), Postives = 31/65 (47.69%), Query Frame = 2


Query: 176 PTEKSYISVLAILVEENQLRLAFRFYRYMRKVGIPPTVASLNVLIKAFCKNSVTMEKAIH 235
           P   SY +++ +L    Q+ LAF F   M K G  P + +L+ L+K       T + A+ 
Sbjct: 279 PNVISYSTLINVLCNSGQIELAFSFLTQMLKRGCHPNIYTLSSLVKGCFLRGTTFD-ALD 338

Query: 236 IFREM 295
           ++ +M
Sbjct: 339 LWNQM 342

Query: 296  355
           
Sbjct: 399  342

Query: 356  371
           
Sbjct: 459  342

[Close]

BLAST of WMU53301 vs. TAIR10
Match: AT1G74580.1 (AT1G74580.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 47.4 bits (111), Expect = 7.7e-06
Identity = 24/71 (33.80%), Postives = 37/71 (52.11%), Query Frame = 2


Query: 164 FHCKPTEKSYISVLAILVEENQLRLAFRFYRYMRKVGIPPTVASLNVLIKAFCKNSVTME 223
           + C+PT  SY +++++LV+      A + Y  MR  GI P V S  + +K+FCK S    
Sbjct: 105 YDCEPTVFSYNAIMSVLVDSGYFDQAHKVYMRMRDRGITPDVYSFTIRMKSFCKTS-RPH 164

Query: 224 KAIHIFREMSN 283
            A+ +   MS+
Sbjct: 165 AALRLLNNMSS 174

Query: 284  343
           
Sbjct: 225  174

Query: 344  377
           
Sbjct: 285  174

[Close]

BLAST of WMU53301 vs. Swiss-Prot
Match: PP418_ARATH (Pentatricopeptide repeat-containing protein At5g46100 OS=Arabidopsis thaliana GN=At5g46100 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 3.9e-20
Identity = 45/73 (61.64%), Postives = 59/73 (80.82%), Query Frame = 2


Query: 152 KCRIFHCKPTEKSYISVLAILVEENQLRLAFRFYRYMRKVGIPPTVASLNVLIKAFCKNS 211
           K + F C P++K+Y++VLAILVEENQL LAF+FY+ MR++G+PPTVASLNVLIKA C+N 
Sbjct: 111 KMKDFDCDPSQKAYVTVLAILVEENQLNLAFKFYKNMREIGLPPTVASLNVLIKALCRND 170

Query: 212 VTMEKAIHIFREM 271
            T++  + IF EM
Sbjct: 171 GTVDAGLKIFLEM 183

Query: 272  331
           
Sbjct: 231  183

Query: 332  371
           
Sbjct: 291  183

HSP 2 Score: 81.6 bits (200), Expect = 6.5e-15
Identity = 38/55 (69.09%), Postives = 44/55 (80.00%), Query Frame = 1


Query: 1   LVSANQFRLAETLLDRMKEEKIDVTEDILLSICRAYGRIHKPLDSIRVFHKMQDF 60
           LVSAN+F+ AE L+ RMK E   V+EDILLSICR YGR+H+P DS+RVFHKM+DF
Sbjct: 61  LVSANKFKAAEDLIVRMKIENCVVSEDILLSICRGYGRVHRPFDSLRVFHKMKDF 115

Query: 61   120
           
Sbjct: 121  115

Query: 121  166
           
Sbjct: 181  115

[Close]

BLAST of WMU53301 vs. Swiss-Prot
Match: PP270_ARATH (Pentatricopeptide repeat-containing protein At3g48810 OS=Arabidopsis thaliana GN=At3g48810 PE=2 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 3.8e-07
Identity = 30/79 (37.97%), Postives = 46/79 (58.23%), Query Frame = 2


Query: 140 EFSIKCRIFHCKPTEKSYISVLAILVEENQLRLAFRFYRYMRKVGIPPTVASLNVLIKAF 199
           E   + + F C P+ K Y  VL  L+ EN++++ +  YR M++ G  P V + NVL+KA 
Sbjct: 132 EMFYRIKEFGCDPSVKIYNHVLDTLLGENRIQMIYMVYRDMKRDGFEPNVFTYNVLLKAL 191

Query: 200 CKNSVTMEKAIHIFREMSN 259
           CKN+  ++ A  +  EMSN
Sbjct: 192 CKNN-KVDGAKKLLVEMSN 209

Query: 260  319
           
Sbjct: 252  209

Query: 320  377
           
Sbjct: 312  209

HSP 2 Score: 34.7 bits (78), Expect = 9.1e-01
Identity = 19/65 (29.23%), Postives = 31/65 (47.69%), Query Frame = 2


Query: 176 PTEKSYISVLAILVEENQLRLAFRFYRYMRKVGIPPTVASLNVLIKAFCKNSVTMEKAIH 235
           P   SY +++ +L    Q+ LAF F   M K G  P + +L+ L+K       T + A+ 
Sbjct: 279 PNVISYSTLINVLCNSGQIELAFSFLTQMLKRGCHPNIYTLSSLVKGCFLRGTTFD-ALD 338

Query: 236 IFREM 295
           ++ +M
Sbjct: 339 LWNQM 342

Query: 296  355
           
Sbjct: 399  342

Query: 356  371
           
Sbjct: 459  342

[Close]

BLAST of WMU53301 vs. NCBI nr
Match: gi|659068118|ref|XP_008442845.1| (PREDICTED: pentatricopeptide repeat-containing protein At5g46100 isoform X1 [Cucumis melo])

HSP 1 Score: 128.6 bits (322), Expect = 7.4e-27
Identity = 62/71 (87.32%), Postives = 67/71 (94.37%), Query Frame = 2


Query: 164 FHCKPTEKSYISVLAILVEENQLRLAFRFYRYMRKVGIPPTVASLNVLIKAFCKNSVTME 223
           FHCKPTEKSYISVLAILVEENQL+LAFRFYR MRK+GIPPTV SLNVLIKAFCKNS TM+
Sbjct: 114 FHCKPTEKSYISVLAILVEENQLKLAFRFYRDMRKMGIPPTVTSLNVLIKAFCKNSGTMD 173

Query: 224 KAIHIFREMSN 283
           KA+H+FR MSN
Sbjct: 174 KAMHLFRTMSN 184

Query: 284  343
           
Sbjct: 234  184

Query: 344  377
           
Sbjct: 294  184

[Close]

BLAST of WMU53301 vs. NCBI nr
Match: gi|778658822|ref|XP_004146658.2| (PREDICTED: pentatricopeptide repeat-containing protein At5g46100 [Cucumis sativus])

HSP 1 Score: 127.5 bits (319), Expect = 1.7e-26
Identity = 62/75 (82.67%), Postives = 68/75 (90.67%), Query Frame = 2


Query: 152 KCRIFHCKPTEKSYISVLAILVEENQLRLAFRFYRYMRKVGIPPTVASLNVLIKAFCKNS 211
           K + FHCKPTEKSYISVLAILVEENQL+ AFRFYR MRK+GIPPTV SLNVLIKAFCKNS
Sbjct: 110 KMQDFHCKPTEKSYISVLAILVEENQLKSAFRFYRDMRKMGIPPTVTSLNVLIKAFCKNS 169

Query: 212 VTMEKAIHIFREMSN 271
            TM+KA+H+FR MSN
Sbjct: 170 GTMDKAMHLFRTMSN 184

Query: 272  331
           
Sbjct: 230  184

Query: 332  377
           
Sbjct: 290  184

[Close]

BLAST of WMU53301 vs. NCBI nr
Match: gi|700209609|gb|KGN64705.1| (hypothetical protein Csa_1G075590 [Cucumis sativus])

HSP 1 Score: 127.5 bits (319), Expect = 1.7e-26
Identity = 62/75 (82.67%), Postives = 68/75 (90.67%), Query Frame = 2


Query: 152 KCRIFHCKPTEKSYISVLAILVEENQLRLAFRFYRYMRKVGIPPTVASLNVLIKAFCKNS 211
           K + FHCKPTEKSYISVLAILVEENQL+ AFRFYR MRK+GIPPTV SLNVLIKAFCKNS
Sbjct: 217 KMQDFHCKPTEKSYISVLAILVEENQLKSAFRFYRDMRKMGIPPTVTSLNVLIKAFCKNS 276

Query: 212 VTMEKAIHIFREMSN 271
            TM+KA+H+FR MSN
Sbjct: 277 GTMDKAMHLFRTMSN 291

Query: 272  331
           
Sbjct: 337  291

Query: 332  377
           
Sbjct: 397  291

[Close]

BLAST of WMU53301 vs. NCBI nr
Match: gi|567914199|ref|XP_006449413.1| (hypothetical protein CICLE_v10015063mg [Citrus clementina])

HSP 1 Score: 111.3 bits (277), Expect = 1.2e-21
Identity = 55/75 (73.33%), Postives = 63/75 (84.00%), Query Frame = 2


Query: 152 KCRIFHCKPTEKSYISVLAILVEENQLRLAFRFYRYMRKVGIPPTVASLNVLIKAFCKNS 211
           K + F C+PT KSYI+VLAILVEENQL+LAFRFYR+MR++GI P VASLNVLIKA CKNS
Sbjct: 110 KMKEFGCEPTPKSYITVLAILVEENQLKLAFRFYRHMREIGIQPCVASLNVLIKALCKNS 169

Query: 212 VTMEKAIHIFREMSN 271
            TM+ A  IFREM N
Sbjct: 170 GTMDAAFKIFREMPN 184

Query: 272  331
           
Sbjct: 230  184

Query: 332  377
           
Sbjct: 290  184

[Close]

BLAST of WMU53301 vs. NCBI nr
Match: gi|568826734|ref|XP_006467725.1| (PREDICTED: pentatricopeptide repeat-containing protein At5g46100 [Citrus sinensis])

HSP 1 Score: 111.3 bits (277), Expect = 1.2e-21
Identity = 55/75 (73.33%), Postives = 63/75 (84.00%), Query Frame = 2


Query: 152 KCRIFHCKPTEKSYISVLAILVEENQLRLAFRFYRYMRKVGIPPTVASLNVLIKAFCKNS 211
           K + F C+PT KSYI+VLAILVEENQL+LAFRFYR+MR++GI P VASLNVLIKA CKNS
Sbjct: 110 KMKEFGCEPTPKSYITVLAILVEENQLKLAFRFYRHMREIGIQPCVASLNVLIKALCKNS 169

Query: 212 VTMEKAIHIFREMSN 271
            TM+ A  IFREM N
Sbjct: 170 GTMDAAFKIFREMPN 184

Query: 272  331
           
Sbjct: 230  184

Query: 332  377
           
Sbjct: 290  184

The following BLAST results are available for this feature:

BLAST of WMU53301 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G46100.1	2.2e-21	61.64	Pentatricopeptide repeat (PPR) superfamily protein	[more]
AT3G48810.1	2.2e-08	37.97	Pentatricopeptide repeat (PPR) superfamily protein	[more]
AT1G74580.1	7.7e-06	33.80	Pentatricopeptide repeat (PPR) superfamily protein	[more]

BLAST of WMU53301 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PP418_ARATH	3.9e-20	61.64	Pentatricopeptide repeat-containing protein At5g46100 OS=Arabidopsis thaliana GN...	[more]
PP270_ARATH	3.8e-07	37.97	Pentatricopeptide repeat-containing protein At3g48810 OS=Arabidopsis thaliana GN...	[more]

BLAST of WMU53301 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659068118\|ref\|XP_008442845.1\|	7.4e-27	87.32	PREDICTED: pentatricopeptide repeat-containing protein At5g46100 isoform X1 [Cuc...	[more]
gi\|778658822\|ref\|XP_004146658.2\|	1.7e-26	82.67	PREDICTED: pentatricopeptide repeat-containing protein At5g46100 [Cucumis sativu...	[more]
gi\|700209609\|gb\|KGN64705.1\|	1.7e-26	82.67	hypothetical protein Csa_1G075590 [Cucumis sativus]	[more]
gi\|567914199\|ref\|XP_006449413.1\|	1.2e-21	73.33	hypothetical protein CICLE_v10015063mg [Citrus clementina]	[more]
gi\|568826734\|ref\|XP_006467725.1\|	1.2e-21	73.33	PREDICTED: pentatricopeptide repeat-containing protein At5g46100 [Citrus sinensi...	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR002885	Pentatricopeptide_repeat

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Cla019695	Cla019695	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
S1_0083894	S1_0083894	EST

InterPro

Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002885	Pentatricopeptide repeat	PFAM	PF13041	PPR_2	coord: 63..108 score: 2.5
IPR002885	Pentatricopeptide repeat	TIGRFAMs	TIGR00756	TIGR00756	coord: 63..96 score: 0.0012coord: 98..124 score: 3.
IPR002885	Pentatricopeptide repeat	PROFILE	PS51375	PPR	coord: 25..59 score: 5.799coord: 60..94 score: 9.065coord: 95..126 score: 8.835coord: 1..24 score: 5
None	No IPR available	PANTHER	PTHR24015	FAMILY NOT NAMED	coord: 1..125 score: 2.9
None	No IPR available	PANTHER	PTHR24015:SF383	SUBFAMILY NOT NAMED	coord: 1..125 score: 2.9