WMU43778 (transcribed_cluster) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	WMU43778
Type	transcribed_cluster
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Delta(7)-sterol-C5(6)-desaturase
Location	Chr7 : 31334430 .. 31335443 (-)
Sequence length	494

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

GAGACTCTCTCTATTTTCTCCCTTTCAGCATCCTCTGCTTTTGATTTCACTGTTTTTTCTCTGTTTTCCGGTGGCTATTCTCCGACGGACATGGGCGGTGGGCCGCCGTATTTGGAGCTCTTTGTGGAGGAGACTAGTTTCTACAACCGCATTGTTTTGGGAACCATTTTTCCAGCTTTTACTTTAGACCCATTTCCTCATATTGTTCAAACTTGGCTTCGAAACTGCATTGGTGGATTCTTAATCTACTTCTTATCTGCCTTTCTTTGGTGCTTCTACATCTATTACTGGAAGCGCAATGTCTATGTTCCTAAAGATGCTGGTGATTTTTACCTTTTCCACGATGGATGATTGTAACTGATTACTAATCTACAGTATTTTTAGTCATCTACAGGTTCTTGTAGATAGATTTCCTAAAAGGCCTGTATTGATATGTGCAAGCTGATGATTCTTCTTTCTTGTCTTTCCTAGATGAATTGTTATGAAAAGACTGT

Library


Library category	ESTs
watermelon fruit 26d library	2

Homology

[Close]

BLAST of WMU43778 vs. TAIR10
Match: AT3G02580.1 (AT3G02580.1 sterol 1)

HSP 1 Score: 107.1 bits (266), Expect = 1.1e-23
Identity = 48/77 (62.34%), Postives = 54/77 (70.13%), Query Frame = 1


Query: 91  MGGGPPYLELFVEETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGFLIYFLSAFLW 150
           M     YL  FV+ETSFYNRIVL  + PA   +P PH +QTWLRN + G L+YF+S FLW
Sbjct: 1   MAADNAYLMQFVDETSFYNRIVLSHLLPANLWEPLPHFLQTWLRNYLAGTLLYFISGFLW 60

Query: 151 CFYIYYWKRNVYVPKDA 210
           CFYIYY K NVY+PKDA
Sbjct: 61  CFYIYYLKINVYLPKDA 77

Query: 211  270
           
Sbjct: 121  77

Query: 271  322
           
Sbjct: 181  77

[Close]

BLAST of WMU43778 vs. TAIR10
Match: AT3G02590.1 (AT3G02590.1 Fatty acid hydroxylase superfamily protein)

HSP 1 Score: 97.4 bits (241), Expect = 8.5e-21
Identity = 41/71 (57.75%), Postives = 50/71 (70.42%), Query Frame = 1


Query: 109 YLELFVEETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGFLIYFLSAFLWCFYIYY 168
           Y +  V ETSFYNR+VL  + P    +P PH +QTWLRN + G ++YF+S FLWCFYIYY
Sbjct: 8   YNDQIVNETSFYNRMVLSHLLPVNLWEPLPHFLQTWLRNYLAGNILYFISGFLWCFYIYY 67

Query: 169 WKRNVYVPKDA 228
            K NVYVPK++
Sbjct: 68  LKLNVYVPKES 78

Query: 229  288
           
Sbjct: 128  78

Query: 289  322
           
Sbjct: 188  78

[Close]

BLAST of WMU43778 vs. Swiss-Prot
Match: SC5D_TOBAC (Delta(7)-sterol-C5(6)-desaturase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 4.5e-24
Identity = 49/71 (69.01%), Postives = 55/71 (77.46%), Query Frame = 1


Query: 109 YLELFVEETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGFLIYFLSAFLWCFYIYY 168
           YL+ FVEETSFYNR+VLGT  P     P PH++Q WLRN IGG L+YF+S FLWCFYIY+
Sbjct: 4   YLKQFVEETSFYNRLVLGTFMPESWWGPLPHMLQGWLRNYIGGVLLYFISGFLWCFYIYH 63

Query: 169 WKRNVYVPKDA 228
            KRNVY+PKDA
Sbjct: 64  LKRNVYIPKDA 74

Query: 229  288
           
Sbjct: 124  74

Query: 289  322
           
Sbjct: 184  74

[Close]

BLAST of WMU43778 vs. Swiss-Prot
Match: SC5D1_ARATH (Delta(7)-sterol-C5(6)-desaturase 1 OS=Arabidopsis thaliana GN=STE1 PE=1 SV=2)

HSP 1 Score: 107.1 bits (266), Expect = 1.9e-22
Identity = 48/77 (62.34%), Postives = 54/77 (70.13%), Query Frame = 1


Query: 91  MGGGPPYLELFVEETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGFLIYFLSAFLW 150
           M     YL  FV+ETSFYNRIVL  + PA   +P PH +QTWLRN + G L+YF+S FLW
Sbjct: 1   MAADNAYLMQFVDETSFYNRIVLSHLLPANLWEPLPHFLQTWLRNYLAGTLLYFISGFLW 60

Query: 151 CFYIYYWKRNVYVPKDA 210
           CFYIYY K NVY+PKDA
Sbjct: 61  CFYIYYLKINVYLPKDA 77

Query: 211  270
           
Sbjct: 121  77

Query: 271  322
           
Sbjct: 181  77

[Close]

BLAST of WMU43778 vs. Swiss-Prot
Match: SC5D2_ARATH (Putative Delta(7)-sterol-C5(6)-desaturase 2 OS=Arabidopsis thaliana GN=HDF7 PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.5e-19
Identity = 41/71 (57.75%), Postives = 50/71 (70.42%), Query Frame = 1


Query: 109 YLELFVEETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGFLIYFLSAFLWCFYIYY 168
           Y +  V ETSFYNR+VL  + P    +P PH +QTWLRN + G ++YF+S FLWCFYIYY
Sbjct: 8   YNDQIVNETSFYNRMVLSHLLPVNLWEPLPHFLQTWLRNYLAGNILYFISGFLWCFYIYY 67

Query: 169 WKRNVYVPKDA 228
            K NVYVPK++
Sbjct: 68  LKLNVYVPKES 78

Query: 229  288
           
Sbjct: 128  78

Query: 289  322
           
Sbjct: 188  78

[Close]

BLAST of WMU43778 vs. NCBI nr
Match: gi|449465599|ref|XP_004150515.1| (PREDICTED: delta(7)-sterol-C5(6)-desaturase [Cucumis sativus])

HSP 1 Score: 162.5 bits (410), Expect = 6.1e-37
Identity = 70/77 (90.91%), Postives = 74/77 (96.10%), Query Frame = 1


Query: 91  MGGGPPYLELFVEETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGFLIYFLSAFLW 150
           MGGGPPYLELFVEETSFYNRIVLGTIFP F+LDPFPHI+QTWLRNC+GGFLIYF S FLW
Sbjct: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60

Query: 151 CFYIYYWKRNVYVPKDA 210
           CFYIYYWKRNVYVPKD+
Sbjct: 61  CFYIYYWKRNVYVPKDS 77

Query: 211  270
           
Sbjct: 121  77

Query: 271  322
           
Sbjct: 181  77

[Close]

BLAST of WMU43778 vs. NCBI nr
Match: gi|659086753|ref|XP_008444093.1| (PREDICTED: delta(7)-sterol-C5(6)-desaturase-like [Cucumis melo])

HSP 1 Score: 159.1 bits (401), Expect = 6.7e-36
Identity = 69/77 (89.61%), Postives = 73/77 (94.81%), Query Frame = 1


Query: 91  MGGGPPYLELFVEETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGFLIYFLSAFLW 150
           MGGGPPYLELFVEETSFYNRIVLGTIFP F+L PFPHI+QTWLRNC+GGFLIYF S FLW
Sbjct: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLYPFPHILQTWLRNCVGGFLIYFFSGFLW 60

Query: 151 CFYIYYWKRNVYVPKDA 210
           CFYIYYWKRNVYVPKD+
Sbjct: 61  CFYIYYWKRNVYVPKDS 77

Query: 211  270
           
Sbjct: 121  77

Query: 271  322
           
Sbjct: 181  77

[Close]

BLAST of WMU43778 vs. NCBI nr
Match: gi|729380166|ref|XP_010549909.1| (PREDICTED: delta(7)-sterol-C5(6)-desaturase 1-like [Tarenaya hassleriana])

HSP 1 Score: 117.1 bits (292), Expect = 2.9e-23
Identity = 53/77 (68.83%), Postives = 59/77 (76.62%), Query Frame = 1


Query: 91  MGGGPPYLELFVEETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGFLIYFLSAFLW 150
           M G   YL LFV+ETS YNRIVLG + PA   DP PH +QTWLRN + G L+YF+S FLW
Sbjct: 1   MAGEQSYLMLFVDETSLYNRIVLGHLLPANLWDPLPHFLQTWLRNYLAGTLLYFISGFLW 60

Query: 151 CFYIYYWKRNVYVPKDA 210
           CFYIYY KRNVYVPKDA
Sbjct: 61  CFYIYYLKRNVYVPKDA 77

Query: 211  270
           
Sbjct: 121  77

Query: 271  322
           
Sbjct: 181  77

[Close]

BLAST of WMU43778 vs. NCBI nr
Match: gi|947044303|gb|KRG93932.1| (hypothetical protein GLYMA_19G0505002, partial [Glycine max])

HSP 1 Score: 116.7 bits (291), Expect = 3.8e-23
Identity = 50/71 (70.42%), Postives = 55/71 (77.46%), Query Frame = 1


Query: 109 YLELFVEETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGFLIYFLSAFLWCFYIYY 168
           Y  LFVE+T  YNRIVLGT+ P F   P PH +QTWLRN +GG L+Y LS  LWCFYIYY
Sbjct: 16  YSRLFVEDTDLYNRIVLGTLLPHFVWGPLPHFLQTWLRNYLGGVLLYLLSGLLWCFYIYY 75

Query: 169 WKRNVYVPKDA 228
           WKRNVYVPKDA
Sbjct: 76  WKRNVYVPKDA 86

Query: 229  288
           
Sbjct: 136  86

Query: 289  322
           
Sbjct: 196  86

[Close]

BLAST of WMU43778 vs. NCBI nr
Match: gi|255637351|gb|ACU19005.1| (unknown [Glycine max])

HSP 1 Score: 116.7 bits (291), Expect = 3.8e-23
Identity = 50/71 (70.42%), Postives = 55/71 (77.46%), Query Frame = 1


Query: 109 YLELFVEETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGFLIYFLSAFLWCFYIYY 168
           Y  LFVE+T  YNRIVLGT+ P F   P PH +QTWLRN +GG L+Y LS  LWCFYIYY
Sbjct: 16  YSRLFVEDTDLYNRIVLGTLLPHFVWGPLPHFLQTWLRNYLGGVLLYLLSGLLWCFYIYY 75

Query: 169 WKRNVYVPKDA 228
           WKRNVYVPKDA
Sbjct: 76  WKRNVYVPKDA 86

Query: 229  288
           
Sbjct: 136  86

Query: 289  322
           
Sbjct: 196  86

The following BLAST results are available for this feature:

BLAST of WMU43778 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G02580.1	1.1e-23	62.34	sterol 1	[more]
AT3G02590.1	8.5e-21	57.75	Fatty acid hydroxylase superfamily protein	[more]

BLAST of WMU43778 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SC5D_TOBAC	4.5e-24	69.01	Delta(7)-sterol-C5(6)-desaturase OS=Nicotiana tabacum PE=2 SV=1	[more]
SC5D1_ARATH	1.9e-22	62.34	Delta(7)-sterol-C5(6)-desaturase 1 OS=Arabidopsis thaliana GN=STE1 PE=1 SV=2	[more]
SC5D2_ARATH	1.5e-19	57.75	Putative Delta(7)-sterol-C5(6)-desaturase 2 OS=Arabidopsis thaliana GN=HDF7 PE=3...	[more]

BLAST of WMU43778 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449465599\|ref\|XP_004150515.1\|	6.1e-37	90.91	PREDICTED: delta(7)-sterol-C5(6)-desaturase [Cucumis sativus]	[more]
gi\|659086753\|ref\|XP_008444093.1\|	6.7e-36	89.61	PREDICTED: delta(7)-sterol-C5(6)-desaturase-like [Cucumis melo]	[more]
gi\|729380166\|ref\|XP_010549909.1\|	2.9e-23	68.83	PREDICTED: delta(7)-sterol-C5(6)-desaturase 1-like [Tarenaya hassleriana]	[more]
gi\|947044303\|gb\|KRG93932.1\|	3.8e-23	70.42	hypothetical protein GLYMA_19G0505002, partial [Glycine max]	[more]
gi\|255637351\|gb\|ACU19005.1\|	3.8e-23	70.42	unknown [Glycine max]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Cla010940	Cla010940	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
S3_0111900	S3_0111900	EST
S3_0143347	S3_0143347	EST

InterPro

Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR11863	STEROL DESATURASE	coord: 7..77 score: 4.6
None	No IPR available	PANTHER	PTHR11863:SF33	LATHOSTEROL OXIDASE	coord: 7..77 score: 4.6