WMU43497 (transcribed_cluster) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	WMU43497
Type	transcribed_cluster
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Cytochrome P450
Location	Chr5 : 8338335 .. 8339711 (+)
Sequence length	781

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

GAGACGACGGCGCTGGCCATCAGTTGGACGCTGCTGCTGTTGGCCACTCGCCCGGAATGGCAAAACATTTTACGAGAGGAGATTAAAGAAGTGGTCGGAGATAAAGATATTGATTTCTCCATGCTTTCTTCACTCAAAAAGATGGGATGGGTGTGGAATGAGGTACTTAGGCTATACCCCTCAGCCCCAAACATACAAAGACAAGCAAAAGGAGACATTGATTTAGGGGACATGAAGATCCCAAAAGGGACCAACATATGGATAGACATCGTCGCAATGCATCACGACTCGACCCTATGGGGCAACGACGTTAACGAGTTCAAGCCAGAGAGGTTCCAACAAGACACCATTCATGGCGGGTGCAACCACAAGATGGGATATTTGCCTTTTGGTTTTGGAGGGAGAATGTGCATTGGTAGAAACTTATCTTCTATGGAGTATAAGATTGTCCTAACCCTAATTCTTTCTACATTTTCGCTCTCTCTTTCTCCTTCTTACTCTCATTCTCCTGCTAATTTGCTCTCTCTGAGACCTGCCCATGGCATTCCTCTCATTCTAAGCCCGCTTTATCCTTAACTTTTTTCACGTCAATTGCTTTTAATTTTAGCTCAACATAACATTTAGTTGAGCCGAGTATGTATGTTTTAACTAGTTGAGTTATTTTTCTATAATATATATCTTTTTAATTTACGATTCAAGATATGAAGTAAAATAAAATGTCTTCATCTCATAAGGTTAAATGACCTTATTTTGGGAATGTGGGACTATATATTTTTGTTGT

Library


Library category	ESTs
watermelon fruit 10d library	2
Watermelon normalization and subtraction library	2

Homology

[Close]

BLAST of WMU43497 vs. TAIR10
Match: AT5G52400.1 (AT5G52400.1 cytochrome P450, family 715, subfamily A, polypeptide 1)

HSP 1 Score: 239.2 bits (609), Expect = 2.9e-63
Identity = 111/181 (61.33%), Postives = 132/181 (72.93%), Query Frame = 1


Query: 1   ETTALAISWTLLLLATRPEWQNILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYP 60
           ETTALA++WT +LLA  PEWQ+ +REEI+EV+GD  I+++ L+ LKKM WV NEVLRLYP
Sbjct: 332 ETTALALTWTFMLLAIHPEWQDTIREEIREVIGDSKIEYNKLAGLKKMSWVMNEVLRLYP 391

Query: 61  SAPNIQRQAKGDIDLGDMKIPKGTNIWIDIVAMHHDSTLWGNDVNEFKPERFQQDTIHGG 120
            APN QRQA+ DI++    IP GTNIWID+VAMHHD  LWG+DVNEFKPERF    +HGG
Sbjct: 392 PAPNAQRQARNDIEVNGRVIPNGTNIWIDVVAMHHDVELWGDDVNEFKPERF-DGNLHGG 451

Query: 121 CNHKMGYLPFGFGGRMCIGRNLSSMEYKIVXXXXXXXXXXXXXXXXXXXPANLLSLRPAH 180
           C +KMGY+PFGFGGRMCIGRNL++MEYKIV                   P  +LSLRP +
Sbjct: 452 CKNKMGYMPFGFGGRMCIGRNLTTMEYKIVLSLVLSRFEISVSPGYRHSPTYMLSLRPGY 511

Query: 181 G 240
           G
Sbjct: 512 G 511

Query: 241  300
           
Sbjct: 572  511

Query: 301  360
           
Sbjct: 632  511

Query: 361  420
           
Sbjct: 692  511

Query: 421  480
           
Sbjct: 752  511

Query: 481  540
           
Sbjct: 812  511

Query: 541  544
           
Sbjct: 872  511

[Close]

BLAST of WMU43497 vs. TAIR10
Match: AT5G24900.1 (AT5G24900.1 cytochrome P450, family 714, subfamily A, polypeptide 2)

HSP 1 Score: 141.4 bits (355), Expect = 8.1e-34
Identity = 70/181 (38.67%), Postives = 98/181 (54.14%), Query Frame = 1


Query: 1   ETTALAISWTLLLLATRPEWQNILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYP 60
           ++TA+++SW L+LLA  P WQ  +R+EI     +   D   + +LK +  V  E +RLYP
Sbjct: 340 DSTAVSVSWCLMLLALNPSWQVKIRDEILSSCKNGIPDAESIPNLKTVTMVIQETMRLYP 399

Query: 61  SAPNIQRQAKGDIDLGDMKIPKGTNIWIDIVAMHHDSTLWGNDVNEFKPERFQQDTIHGG 120
            AP + R+A  DI LGD+ +PKG  IW  I A+H D  +WG D N+FKPERF +  I   
Sbjct: 400 PAPIVGREASKDIRLGDLVVPKGVCIWTLIPALHRDPEIWGPDANDFKPERFSEG-ISKA 459

Query: 121 CNHKMGYLPFGFGGRMCIGRNLSSMEYKIVXXXXXXXXXXXXXXXXXXXPANLLSLRPAH 180
           C +   Y+PFG G R C+G+N   ME K++                   P++ L + P H
Sbjct: 460 CKYPQSYIPFGLGPRTCVGKNFGMMEVKVLVSLIVSKFSFTLSPTYQHSPSHKLLVEPQH 519

Query: 181 G 240
           G
Sbjct: 520 G 519

Query: 241  300
           
Sbjct: 580  519

Query: 301  360
           
Sbjct: 640  519

Query: 361  420
           
Sbjct: 700  519

Query: 421  480
           
Sbjct: 760  519

Query: 481  540
           
Sbjct: 820  519

Query: 541  544
           
Sbjct: 880  519

[Close]

BLAST of WMU43497 vs. TAIR10
Match: AT2G26710.1 (AT2G26710.1 Cytochrome P450 superfamily protein)

HSP 1 Score: 141.4 bits (355), Expect = 8.1e-34
Identity = 72/182 (39.56%), Postives = 99/182 (54.40%), Query Frame = 1


Query: 1   ETTALAISWTLLLLATRPEWQNILREEIKEVVGDKDIDFS-MLSSLKKMGWVWNEVLRLY 60
           +TT+  ++WT +LL+  PEWQ   R+E+  V G +D+     +  LK +  + NE LRLY
Sbjct: 327 QTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSRDVPTKDHVVKLKTLSMILNESLRLY 386

Query: 61  PSAPNIQRQAKGDIDLGDMKIPKGTNIWIDIVAMHHDSTLWGNDVNEFKPERFQQDTIHG 120
           P      R+AK D+ LG  KIP GT + I I+A+HHD  +WGNDVNEF P RF  D +  
Sbjct: 387 PPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQAIWGNDVNEFNPARF-ADGVPR 446

Query: 121 GCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVXXXXXXXXXXXXXXXXXXXPANLLSLRPA 180
              H +G++PFG G R CIG+NL+ ++ K+                    P  L+ L P 
Sbjct: 447 AAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTFHLAPTYQHAPTVLMLLYPQ 506

Query: 181 HG 240
           HG
Sbjct: 507 HG 507

Query: 241  300
           
Sbjct: 567  507

Query: 301  360
           
Sbjct: 627  507

Query: 361  420
           
Sbjct: 687  507

Query: 421  480
           
Sbjct: 747  507

Query: 481  540
           
Sbjct: 807  507

Query: 541  544
           
Sbjct: 867  507

[Close]

BLAST of WMU43497 vs. TAIR10
Match: AT1G67110.1 (AT1G67110.1 cytochrome P450, family 735, subfamily A, polypeptide 2)

HSP 1 Score: 139.8 bits (351), Expect = 2.4e-33
Identity = 74/182 (40.66%), Postives = 102/182 (56.04%), Query Frame = 1


Query: 1   ETTALAISWTLLLLATRPEWQNILREEIKEVVGDKDI-DFSMLSSLKKMGWVWNEVLRLY 60
           ETT+L ++WTL+LLA  P WQ+ +R+E+++V G   +     LSSL  +  V NE LRLY
Sbjct: 325 ETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLY 384

Query: 61  PSAPNIQRQAKGDIDLGDMKIPKGTNIWIDIVAMHHDSTLWGNDVNEFKPERFQQDTIHG 120
           P A  + R A  DI LGD+ IPKG +IWI ++A+HH + LWG D NEF PERF   +   
Sbjct: 385 PPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSF-A 444

Query: 121 GCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVXXXXXXXXXXXXXXXXXXXPANLLSLRPA 180
              H   ++PF  G R CIG+  + ME KI+                   P  +L+++P 
Sbjct: 445 SSRH---FMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTIKPK 502

Query: 181 HG 240
           +G
Sbjct: 505 YG 502

Query: 241  300
           
Sbjct: 565  502

Query: 301  360
           
Sbjct: 625  502

Query: 361  420
           
Sbjct: 685  502

Query: 421  480
           
Sbjct: 745  502

Query: 481  540
           
Sbjct: 805  502

Query: 541  544
           
Sbjct: 865  502

[Close]

BLAST of WMU43497 vs. TAIR10
Match: AT5G38450.1 (AT5G38450.1 cytochrome P450, family 735, subfamily A, polypeptide 1)

HSP 1 Score: 137.9 bits (346), Expect = 9.0e-33
Identity = 75/184 (40.76%), Postives = 99/184 (53.80%), Query Frame = 1


Query: 1   ETTALAISWTLLLLATRPEWQNILREEIKEVVGDKDI-DFSMLSSLKKMGWVWNEVLRLY 60
           ETTAL ++WT +LLA  P WQ  +REE++EV G   +     LS L  +  V NE LRLY
Sbjct: 331 ETTALLLTWTTMLLADNPTWQEKVREEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLY 390

Query: 61  PSAPNIQRQAKGDIDLGDMKIPKGTNIWIDIVAMHHDSTLWGNDVNEFKPERFQQDTIHG 120
           P A  + R A  D+ LGD+ IPKG +IWI ++A+HH   LWG D N+F PERF      G
Sbjct: 391 PPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIHHSEELWGKDANQFNPERF------G 450

Query: 121 GCNHKMG--YLPFGFGGRMCIGRNLSSMEYKIVXXXXXXXXXXXXXXXXXXXPANLLSLR 180
           G     G  ++PF  G R CIG+  + ME KI+                   P  +L+++
Sbjct: 451 GRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILATLISKFNFTISKNYRHAPIVVLTIK 508

Query: 181 PAHG 240
           P +G
Sbjct: 511 PKYG 508

Query: 241  300
           
Sbjct: 571  508

Query: 301  360
           
Sbjct: 631  508

Query: 361  420
           
Sbjct: 691  508

Query: 421  480
           
Sbjct: 751  508

Query: 481  540
           
Sbjct: 811  508

Query: 541  544
           
Sbjct: 871  508

[Close]

BLAST of WMU43497 vs. Swiss-Prot
Match: C14A2_ARATH (Cytochrome P450 714A2 OS=Arabidopsis thaliana GN=CYP714A2 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.4e-32
Identity = 70/181 (38.67%), Postives = 98/181 (54.14%), Query Frame = 1


Query: 1   ETTALAISWTLLLLATRPEWQNILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYP 60
           ++TA+++SW L+LLA  P WQ  +R+EI     +   D   + +LK +  V  E +RLYP
Sbjct: 340 DSTAVSVSWCLMLLALNPSWQVKIRDEILSSCKNGIPDAESIPNLKTVTMVIQETMRLYP 399

Query: 61  SAPNIQRQAKGDIDLGDMKIPKGTNIWIDIVAMHHDSTLWGNDVNEFKPERFQQDTIHGG 120
            AP + R+A  DI LGD+ +PKG  IW  I A+H D  +WG D N+FKPERF +  I   
Sbjct: 400 PAPIVGREASKDIRLGDLVVPKGVCIWTLIPALHRDPEIWGPDANDFKPERFSEG-ISKA 459

Query: 121 CNHKMGYLPFGFGGRMCIGRNLSSMEYKIVXXXXXXXXXXXXXXXXXXXPANLLSLRPAH 180
           C +   Y+PFG G R C+G+N   ME K++                   P++ L + P H
Sbjct: 460 CKYPQSYIPFGLGPRTCVGKNFGMMEVKVLVSLIVSKFSFTLSPTYQHSPSHKLLVEPQH 519

Query: 181 G 240
           G
Sbjct: 520 G 519

Query: 241  300
           
Sbjct: 580  519

Query: 301  360
           
Sbjct: 640  519

Query: 361  420
           
Sbjct: 700  519

Query: 421  480
           
Sbjct: 760  519

Query: 481  540
           
Sbjct: 820  519

Query: 541  544
           
Sbjct: 880  519

[Close]

BLAST of WMU43497 vs. Swiss-Prot
Match: C734A_ARATH (Cytochrome P450 734A1 OS=Arabidopsis thaliana GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.4e-32
Identity = 72/182 (39.56%), Postives = 99/182 (54.40%), Query Frame = 1


Query: 1   ETTALAISWTLLLLATRPEWQNILREEIKEVVGDKDIDFS-MLSSLKKMGWVWNEVLRLY 60
           +TT+  ++WT +LL+  PEWQ   R+E+  V G +D+     +  LK +  + NE LRLY
Sbjct: 327 QTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSRDVPTKDHVVKLKTLSMILNESLRLY 386

Query: 61  PSAPNIQRQAKGDIDLGDMKIPKGTNIWIDIVAMHHDSTLWGNDVNEFKPERFQQDTIHG 120
           P      R+AK D+ LG  KIP GT + I I+A+HHD  +WGNDVNEF P RF  D +  
Sbjct: 387 PPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQAIWGNDVNEFNPARF-ADGVPR 446

Query: 121 GCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVXXXXXXXXXXXXXXXXXXXPANLLSLRPA 180
              H +G++PFG G R CIG+NL+ ++ K+                    P  L+ L P 
Sbjct: 447 AAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTFHLAPTYQHAPTVLMLLYPQ 506

Query: 181 HG 240
           HG
Sbjct: 507 HG 507

Query: 241  300
           
Sbjct: 567  507

Query: 301  360
           
Sbjct: 627  507

Query: 361  420
           
Sbjct: 687  507

Query: 421  480
           
Sbjct: 747  507

Query: 481  540
           
Sbjct: 807  507

Query: 541  544
           
Sbjct: 867  507

[Close]

BLAST of WMU43497 vs. Swiss-Prot
Match: C7352_ARATH (Cytokinin hydroxylase OS=Arabidopsis thaliana GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 4.2e-32
Identity = 74/182 (40.66%), Postives = 102/182 (56.04%), Query Frame = 1


Query: 1   ETTALAISWTLLLLATRPEWQNILREEIKEVVGDKDI-DFSMLSSLKKMGWVWNEVLRLY 60
           ETT+L ++WTL+LLA  P WQ+ +R+E+++V G   +     LSSL  +  V NE LRLY
Sbjct: 325 ETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLY 384

Query: 61  PSAPNIQRQAKGDIDLGDMKIPKGTNIWIDIVAMHHDSTLWGNDVNEFKPERFQQDTIHG 120
           P A  + R A  DI LGD+ IPKG +IWI ++A+HH + LWG D NEF PERF   +   
Sbjct: 385 PPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSF-A 444

Query: 121 GCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVXXXXXXXXXXXXXXXXXXXPANLLSLRPA 180
              H   ++PF  G R CIG+  + ME KI+                   P  +L+++P 
Sbjct: 445 SSRH---FMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTIKPK 502

Query: 181 HG 240
           +G
Sbjct: 505 YG 502

Query: 241  300
           
Sbjct: 565  502

Query: 301  360
           
Sbjct: 625  502

Query: 361  420
           
Sbjct: 685  502

Query: 421  480
           
Sbjct: 745  502

Query: 481  540
           
Sbjct: 805  502

Query: 541  544
           
Sbjct: 865  502

[Close]

BLAST of WMU43497 vs. Swiss-Prot
Match: C7351_ARATH (Cytokinin hydroxylase OS=Arabidopsis thaliana GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.6e-31
Identity = 75/184 (40.76%), Postives = 99/184 (53.80%), Query Frame = 1


Query: 1   ETTALAISWTLLLLATRPEWQNILREEIKEVVGDKDI-DFSMLSSLKKMGWVWNEVLRLY 60
           ETTAL ++WT +LLA  P WQ  +REE++EV G   +     LS L  +  V NE LRLY
Sbjct: 331 ETTALLLTWTTMLLADNPTWQEKVREEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLY 390

Query: 61  PSAPNIQRQAKGDIDLGDMKIPKGTNIWIDIVAMHHDSTLWGNDVNEFKPERFQQDTIHG 120
           P A  + R A  D+ LGD+ IPKG +IWI ++A+HH   LWG D N+F PERF      G
Sbjct: 391 PPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIHHSEELWGKDANQFNPERF------G 450

Query: 121 GCNHKMG--YLPFGFGGRMCIGRNLSSMEYKIVXXXXXXXXXXXXXXXXXXXPANLLSLR 180
           G     G  ++PF  G R CIG+  + ME KI+                   P  +L+++
Sbjct: 451 GRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILATLISKFNFTISKNYRHAPIVVLTIK 508

Query: 181 PAHG 240
           P +G
Sbjct: 511 PKYG 508

Query: 241  300
           
Sbjct: 571  508

Query: 301  360
           
Sbjct: 631  508

Query: 361  420
           
Sbjct: 691  508

Query: 421  480
           
Sbjct: 751  508

Query: 481  540
           
Sbjct: 811  508

Query: 541  544
           
Sbjct: 871  508

[Close]

BLAST of WMU43497 vs. Swiss-Prot
Match: C14C2_ORYSJ (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 4.6e-31
Identity = 71/181 (39.23%), Postives = 97/181 (53.59%), Query Frame = 1


Query: 1   ETTALAISWTLLLLATRPEWQNILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYP 60
           ETT+   +W L+LLA+  EWQ+  R E  ++   + +DF +L  LKK+  V  E LRLYP
Sbjct: 335 ETTSTTAAWCLMLLASHHEWQSRARVESLDICQGRPLDFDILRKLKKLTMVIQETLRLYP 394

Query: 61  SAPNIQRQAKGDIDLGDMKIPKGTNIWIDIVAMHHDSTLWGNDVNEFKPERFQQDTIHGG 120
            A  + R+A  D+ LG + IPKGTNIWI I   H D ++WG   ++F P+RF  + I G 
Sbjct: 395 PASFVAREALNDMKLGGIDIPKGTNIWIPIAMAHRDPSVWGPSADKFDPDRF-ANGIAGA 454

Query: 121 CNHKMGYLPFGFGGRMCIGRNLSSMEYKIVXXXXXXXXXXXXXXXXXXXPANLLSLRPAH 180
           C     Y+PFG G R C G+NL+ +E K+V                   PA  L++ P  
Sbjct: 455 CKPPHMYMPFGVGVRTCAGQNLAMVELKVVLSLLLSKFEFKLSPNYVHCPAFRLTIEPGK 514

Query: 181 G 240
           G
Sbjct: 515 G 514

Query: 241  300
           
Sbjct: 575  514

Query: 301  360
           
Sbjct: 635  514

Query: 361  420
           
Sbjct: 695  514

Query: 421  480
           
Sbjct: 755  514

Query: 481  540
           
Sbjct: 815  514

Query: 541  544
           
Sbjct: 875  514

[Close]

BLAST of WMU43497 vs. NCBI nr
Match: gi|778679591|ref|XP_004151435.2| (PREDICTED: cytokinin hydroxylase [Cucumis sativus])

HSP 1 Score: 318.2 bits (814), Expect = 1.4e-83
Identity = 150/181 (82.87%), Postives = 156/181 (86.19%), Query Frame = 1


Query: 1   ETTALAISWTLLLLATRPEWQNILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYP 60
           ETTALAISWTLLLLAT P+WQ ILR+EIKEV+GDKDIDFSMLSSLKKMGWVWNEVLRLYP
Sbjct: 335 ETTALAISWTLLLLATSPDWQTILRDEIKEVIGDKDIDFSMLSSLKKMGWVWNEVLRLYP 394

Query: 61  SAPNIQRQAKGDIDLGDMKIPKGTNIWIDIVAMHHDSTLWGNDVNEFKPERFQQDTIHGG 120
           SAPNIQRQAKGDIDLGD+KIPKGTNIWIDIVAMHHD TLWG+DVNEF P+RFQ DTIHGG
Sbjct: 395 SAPNIQRQAKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGG 454

Query: 121 CNHKMGYLPFGFGGRMCIGRNLSSMEYKIVXXXXXXXXXXXXXXXXXXXPANLLSLRPAH 180
           CNHKMGYLPFGFGGRMCIGRNLSSMEYKIV                   PA LLSLRPAH
Sbjct: 455 CNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYTHSPATLLSLRPAH 514

Query: 181 G 240
           G
Sbjct: 515 G 515

Query: 241  300
           
Sbjct: 575  515

Query: 301  360
           
Sbjct: 635  515

Query: 361  420
           
Sbjct: 695  515

Query: 421  480
           
Sbjct: 755  515

Query: 481  540
           
Sbjct: 815  515

Query: 541  544
           
Sbjct: 875  515

[Close]

BLAST of WMU43497 vs. NCBI nr
Match: gi|659077497|ref|XP_008439236.1| (PREDICTED: cytokinin hydroxylase [Cucumis melo])

HSP 1 Score: 314.7 bits (805), Expect = 1.5e-82
Identity = 149/181 (82.32%), Postives = 154/181 (85.08%), Query Frame = 1


Query: 1   ETTALAISWTLLLLATRPEWQNILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYP 60
           ETTALAISWTLLLLAT P+WQ ILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYP
Sbjct: 335 ETTALAISWTLLLLATSPDWQRILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYP 394

Query: 61  SAPNIQRQAKGDIDLGDMKIPKGTNIWIDIVAMHHDSTLWGNDVNEFKPERFQQDTIHGG 120
           SAPNIQRQAKGDIDLGD+KIPKGTNIWID+VAMHHD TLWG+DVN+F P RFQ DTIHGG
Sbjct: 395 SAPNIQRQAKGDIDLGDIKIPKGTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGG 454

Query: 121 CNHKMGYLPFGFGGRMCIGRNLSSMEYKIVXXXXXXXXXXXXXXXXXXXPANLLSLRPAH 180
           C HKMGYLPFGFGGRMCIGRNLSSMEYKIV                   PA LLSLRPAH
Sbjct: 455 CYHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAH 514

Query: 181 G 240
           G
Sbjct: 515 G 515

Query: 241  300
           
Sbjct: 575  515

Query: 301  360
           
Sbjct: 635  515

Query: 361  420
           
Sbjct: 695  515

Query: 421  480
           
Sbjct: 755  515

Query: 481  540
           
Sbjct: 815  515

Query: 541  544
           
Sbjct: 875  515

[Close]

BLAST of WMU43497 vs. NCBI nr
Match: gi|1009122703|ref|XP_015878144.1| (PREDICTED: cytokinin hydroxylase-like [Ziziphus jujuba])

HSP 1 Score: 262.3 bits (669), Expect = 8.9e-67
Identity = 122/181 (67.40%), Postives = 141/181 (77.90%), Query Frame = 1


Query: 1   ETTALAISWTLLLLATRPEWQNILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYP 60
           ETTALAI+WTLLLLA  PEWQ  LREEIKEV+GDK+ID S+LS LKKMGWV +E+LRLYP
Sbjct: 331 ETTALAITWTLLLLAIHPEWQTQLREEIKEVIGDKEIDASLLSGLKKMGWVLSEILRLYP 390

Query: 61  SAPNIQRQAKGDIDLGDMKIPKGTNIWIDIVAMHHDSTLWGNDVNEFKPERFQQDTIHGG 120
           SAPN+QRQA+GDI + D+ IP GTN+WID+VAMHHD  LWG DVNEFKP+RF+ D I+GG
Sbjct: 391 SAPNVQRQARGDIQVNDLTIPDGTNMWIDVVAMHHDPNLWGEDVNEFKPQRFKDD-INGG 450

Query: 121 CNHKMGYLPFGFGGRMCIGRNLSSMEYKIVXXXXXXXXXXXXXXXXXXXPANLLSLRPAH 180
           C HKMGYLPFGFG RMC+GRNL+ MEYKIV                   P+ +LSLRP H
Sbjct: 451 CKHKMGYLPFGFGARMCVGRNLTFMEYKIVLTLILSRFSFTLSPNYSHSPSIMLSLRPIH 510

Query: 181 G 240
           G
Sbjct: 511 G 510

Query: 241  300
           
Sbjct: 571  510

Query: 301  360
           
Sbjct: 631  510

Query: 361  420
           
Sbjct: 691  510

Query: 421  480
           
Sbjct: 751  510

Query: 481  540
           
Sbjct: 811  510

Query: 541  544
           
Sbjct: 871  510

[Close]

BLAST of WMU43497 vs. NCBI nr
Match: gi|395146537|gb|AFN53691.1| (cytochrome P450 [Linum usitatissimum])

HSP 1 Score: 261.5 bits (667), Expect = 1.5e-66
Identity = 123/181 (67.96%), Postives = 141/181 (77.90%), Query Frame = 1


Query: 1   ETTALAISWTLLLLATRPEWQNILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYP 60
           ETTALAISWTL+LLA  PEWQ+ LR+EIKEV  DK +DFSMLSSLKKMGWV NEVLRLY 
Sbjct: 323 ETTALAISWTLMLLAKHPEWQDELRKEIKEVSKDKMVDFSMLSSLKKMGWVMNEVLRLYS 382

Query: 61  SAPNIQRQAKGDIDLGDMKIPKGTNIWIDIVAMHHDSTLWGNDVNEFKPERFQQDTIHGG 120
            APN+QRQAK DI + D  IPKGTN+WID+VAMHHD TLWG+DV EFKPERF+ D ++GG
Sbjct: 383 PAPNVQRQAKEDIKVFDRTIPKGTNMWIDVVAMHHDRTLWGDDVYEFKPERFKADPLYGG 442

Query: 121 CNHKMGYLPFGFGGRMCIGRNLSSMEYKIVXXXXXXXXXXXXXXXXXXXPANLLSLRPAH 180
           C HKMG++PFGFGGRMC+GRNL+ MEYKIV                   PA +LSLRP+H
Sbjct: 443 CKHKMGFMPFGFGGRMCVGRNLTMMEYKIVLSLILTRFSFSLSPSYDHSPAIVLSLRPSH 502

Query: 181 G 240
           G
Sbjct: 503 G 503

Query: 241  300
           
Sbjct: 563  503

Query: 301  360
           
Sbjct: 623  503

Query: 361  420
           
Sbjct: 683  503

Query: 421  480
           
Sbjct: 743  503

Query: 481  540
           
Sbjct: 803  503

Query: 541  544
           
Sbjct: 863  503

[Close]

BLAST of WMU43497 vs. NCBI nr
Match: gi|743857201|ref|XP_011030117.1| (PREDICTED: cytokinin hydroxylase-like isoform X1 [Populus euphratica])

HSP 1 Score: 260.4 bits (664), Expect = 3.4e-66
Identity = 121/181 (66.85%), Postives = 141/181 (77.90%), Query Frame = 1


Query: 1   ETTALAISWTLLLLATRPEWQNILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYP 60
           ETTALA+SWTLLLLA  PEWQNILREEI+EV GDK+IDF+ ++ LKKMGWV NEVLRLYP
Sbjct: 344 ETTALALSWTLLLLAMHPEWQNILREEIREVTGDKEIDFTKVAGLKKMGWVMNEVLRLYP 403

Query: 61  SAPNIQRQAKGDIDLGDMKIPKGTNIWIDIVAMHHDSTLWGNDVNEFKPERFQQDTIHGG 120
            +PN+QRQA+ DI + D+ IP GTN+WID+VAMHHD  LWG D NEFKPERF+ D ++GG
Sbjct: 404 PSPNVQRQAREDIRVNDLLIPNGTNMWIDVVAMHHDPKLWGEDANEFKPERFKDD-LYGG 463

Query: 121 CNHKMGYLPFGFGGRMCIGRNLSSMEYKIVXXXXXXXXXXXXXXXXXXXPANLLSLRPAH 180
           C HKMG+LPFGFGGRMCIGRNL++MEYKIV                   PA LLSLRP +
Sbjct: 464 CRHKMGFLPFGFGGRMCIGRNLTAMEYKIVLTLILSRFSFSVSPTYSHSPAILLSLRPGN 523

Query: 181 G 240
           G
Sbjct: 524 G 523

Query: 241  300
           
Sbjct: 584  523

Query: 301  360
           
Sbjct: 644  523

Query: 361  420
           
Sbjct: 704  523

Query: 421  480
           
Sbjct: 764  523

Query: 481  540
           
Sbjct: 824  523

Query: 541  544
           
Sbjct: 884  523

The following BLAST results are available for this feature:

BLAST of WMU43497 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G52400.1	2.9e-63	61.33	cytochrome P450, family 715, subfamily A, polypeptide 1	[more]
AT5G24900.1	8.1e-34	38.67	cytochrome P450, family 714, subfamily A, polypeptide 2	[more]
AT2G26710.1	8.1e-34	39.56	Cytochrome P450 superfamily protein	[more]
AT1G67110.1	2.4e-33	40.66	cytochrome P450, family 735, subfamily A, polypeptide 2	[more]
AT5G38450.1	9.0e-33	40.76	cytochrome P450, family 735, subfamily A, polypeptide 1	[more]

BLAST of WMU43497 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C14A2_ARATH	1.4e-32	38.67	Cytochrome P450 714A2 OS=Arabidopsis thaliana GN=CYP714A2 PE=2 SV=1	[more]
C734A_ARATH	1.4e-32	39.56	Cytochrome P450 734A1 OS=Arabidopsis thaliana GN=CYP734A1 PE=2 SV=1	[more]
C7352_ARATH	4.2e-32	40.66	Cytokinin hydroxylase OS=Arabidopsis thaliana GN=CYP735A2 PE=1 SV=1	[more]
C7351_ARATH	1.6e-31	40.76	Cytokinin hydroxylase OS=Arabidopsis thaliana GN=CYP735A1 PE=1 SV=1	[more]
C14C2_ORYSJ	4.6e-31	39.23	Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica GN=CYP714C2 PE=2 SV=1	[more]

BLAST of WMU43497 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778679591\|ref\|XP_004151435.2\|	1.4e-83	82.87	PREDICTED: cytokinin hydroxylase [Cucumis sativus]	[more]
gi\|659077497\|ref\|XP_008439236.1\|	1.5e-82	82.32	PREDICTED: cytokinin hydroxylase [Cucumis melo]	[more]
gi\|1009122703\|ref\|XP_015878144.1\|	8.9e-67	67.40	PREDICTED: cytokinin hydroxylase-like [Ziziphus jujuba]	[more]
gi\|395146537\|gb\|AFN53691.1\|	1.5e-66	67.96	cytochrome P450 [Linum usitatissimum]	[more]
gi\|743857201\|ref\|XP_011030117.1\|	3.4e-66	66.85	PREDICTED: cytokinin hydroxylase-like isoform X1 [Populus euphratica]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450
IPR002401	Cyt_P450_E_grp-I
IPR017972	Cyt_P450_CS

Vocabulary: **Molecular Function**
Term	Definition
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506	iron ion binding
GO:0020037	heme binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Cla006173	Cla006173	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
S1_0068702	S1_0068702	EST
S1_0075821	S1_0075821	EST
WMN07F01	WMN07F01	EST
WMN80A11	WMN80A11	EST

InterPro

Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 50..61 score: 5.3E-8coord: 128..137 score: 5.3E-8coord: 137..148 score: 5.3E-8coord: 16..29 score: 5.
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 1..188 score: 7.0
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 1..165 score: 2.1
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 1..188 score: 4.98
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 137..160 score: 3.2E-14coord: 7..33 score: 3.2E-14coord: 127..137 score: 3.2
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 130..139 scor
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 1..191 score: 4.6E
None	No IPR available	PANTHER	PTHR24282:SF15	CYTOCHROME P450, FAMILY 715, SUBFAMILY A, POLYPEPTIDE 1	coord: 1..191 score: 4.6E