WMU43284 (transcribed_cluster) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	WMU43284
Type	transcribed_cluster
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Unknown protein
Location	Chr0 : 2391424 .. 2391880 (-)
Sequence length	360

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

TGGCGCTCCAACTGCCTTGATCACCAACCACCAGGGGCCCTTCTCCCCATAAAACACCCTATAATTCTCACCCGGAGAAGGCAACCCTTCATCAAGCACAATGGCGACATTCAATTTCTCGAACTCATCAGATTCAGCAAATTTCTCAGCACCGCCATGGCCACCAATTTCTTCATCCGGAACAAAAGATAGGTAAACAGAGCGCAGAGGCTCAAACCCAGATTCCTTCAAACGCCGAATAGCCTCAAGGTACTGCATCCCAACGCACTTCATATCCTGAGACCCTCTGGCATAGATATTGCCATGGGAATCGATATGAGCTCCAAGAGGAGGGTGCGTCCATTTGTGATGCTCAGCTGG

Library


Library category	ESTs
watermelon fruit 34d library	3

Homology

[Close]

BLAST of WMU43284 vs. TAIR10
Match: AT4G38220.2 (AT4G38220.2 Peptidase M20/M25/M40 family protein)

HSP 1 Score: 205.3 bits (521), Expect = 2.1e-53
Identity = 96/120 (80.00%), Postives = 103/120 (85.83%), Query Frame = -1


Query: 1   PAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFV 60
           P E  KWTH PL AH+D HG+IYARGSQDMKCVGMQYLEAIR+L+ SGF+PLRSVYLSFV
Sbjct: 97  PFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLEAIRKLQASGFKPLRSVYLSFV 156

Query: 61  PDEEIGGHGGAEKFAESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKGPWWLVIKAVGAP 120
           PDEEIGGH GAEKFAES  F+ LN+AIVLDEGLPSP E+YRVFYGE+ PWWLVIKA G P
Sbjct: 157 PDEEIGGHDGAEKFAESQLFKSLNIAIVLDEGLPSPTESYRVFYGERSPWWLVIKAKGPP 216

Query: 121  180
           
Sbjct: 217  216

Query: 181  240
           
Sbjct: 277  216

Query: 241  300
           
Sbjct: 337  216

Query: 301  360
           
Sbjct: 397  216

[Close]

BLAST of WMU43284 vs. TAIR10
Match: AT1G44180.1 (AT1G44180.1 Peptidase M20/M25/M40 family protein)

HSP 1 Score: 157.5 bits (397), Expect = 5.0e-39
Identity = 71/120 (59.17%), Postives = 86/120 (71.67%), Query Frame = -1


Query: 1   PAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFV 60
           PAE  KW HPP  AH    G+IYARG+QD KC+G+QYLEAIR LK   F PLR++++S+V
Sbjct: 104 PAESEKWIHPPFSAHRTIDGHIYARGAQDDKCIGVQYLEAIRNLKSRSFSPLRTIHISYV 163

Query: 61  PDEEIGGHGGAEKFAESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKGPWWLVIKAVGAP 120
           P+EEIGG GG  KFA S EF+ LN+  V+DEG  SPG+ +RVFY E+ PW L I+A G P
Sbjct: 164 PEEEIGGFGGMMKFAASSEFKDLNLGFVMDEGQASPGDEFRVFYAERTPWHLAIRANGMP 223

Query: 121  180
           
Sbjct: 224  223

Query: 181  240
           
Sbjct: 284  223

Query: 241  300
           
Sbjct: 344  223

Query: 301  360
           
Sbjct: 404  223

[Close]

BLAST of WMU43284 vs. TAIR10
Match: AT1G44820.1 (AT1G44820.1 Peptidase M20/M25/M40 family protein)

HSP 1 Score: 153.3 bits (386), Expect = 9.5e-38
Identity = 68/120 (56.67%), Postives = 86/120 (71.67%), Query Frame = -1


Query: 1   PAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFV 60
           PAE  KWT+PP  AH    G+IYARG+QD KC+G+QYLE+IR LK  GF PLR++++S+V
Sbjct: 102 PAESEKWTYPPFSAHKTIDGHIYARGAQDDKCIGVQYLESIRNLKSRGFSPLRTIHISYV 161

Query: 61  PDEEIGGHGGAEKFAESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKGPWWLVIKAVGAP 120
           P+EEIGG  G  KFA S EF+ LN+   +DEG  +PG+ +RVFY ++ PW  VIKA G P
Sbjct: 162 PEEEIGGFDGMMKFAASSEFKDLNLGFAMDEGQANPGDEFRVFYADRVPWHFVIKAEGIP 221

Query: 121  180
           
Sbjct: 222  221

Query: 181  240
           
Sbjct: 282  221

Query: 241  300
           
Sbjct: 342  221

Query: 301  360
           
Sbjct: 402  221

[Close]

BLAST of WMU43284 vs. Swiss-Prot
Match: ACY1_DICDI (Aminoacylase-1 OS=Dictyostelium discoideum GN=acy1 PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 1.3e-31
Identity = 65/119 (54.62%), Postives = 82/119 (68.91%), Query Frame = -1


Query: 7   PAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFV 66
           PA H  W   P  A  D  GNI+ RG+QDMKCV MQ+LE  RR+ +SG +  R+++LSFV
Sbjct: 81  PAVHDSWKVDPFSAWKDESGNIFGRGTQDMKCVCMQFLEVARRIVQSGQKLKRTLHLSFV 140

Query: 67  PDEEIGGHG-GAEKFAESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKGPWWLVIKAVG 126
           PDEEIGG G G EKF  +++F +LN+ + LDEGL SP  ++ VFYGE+ PWW+ I AVG
Sbjct: 141 PDEEIGGSGKGMEKFVYTEKFRQLNIGLCLDEGLASPTNDFTVFYGERAPWWVHITAVG 199

Query: 127  186
           
Sbjct: 201  199

Query: 187  246
           
Sbjct: 261  199

Query: 247  306
           
Sbjct: 321  199

Query: 307  361
           
Sbjct: 381  199

[Close]

BLAST of WMU43284 vs. Swiss-Prot
Match: ACY1_HUMAN (Aminoacylase-1 OS=Homo sapiens GN=ACY1 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 8.1e-31
Identity = 59/120 (49.17%), Postives = 76/120 (63.33%), Query Frame = -1


Query: 1   PAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFV 60
           P     W+H P  A  DS G IYARG+QDMKCV +QYLEA+RRLK  G    R+++++FV
Sbjct: 85  PVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCVSIQYLEAVRRLKVEGHRFPRTIHMTFV 144

Query: 61  PDEEIGGHGGAEKFAESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKGPWWLVIKAVGAP 120
           PDEE+GGH G E F +  EF  L     LDEG+ +P + + VFY E+ PWW+ + + G P
Sbjct: 145 PDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPTDAFTVFYSERSPWWVRVTSTGRP 204

Query: 121  180
           
Sbjct: 205  204

Query: 181  240
           
Sbjct: 265  204

Query: 241  300
           
Sbjct: 325  204

Query: 301  360
           
Sbjct: 385  204

[Close]

BLAST of WMU43284 vs. Swiss-Prot
Match: ACY1_PONAB (Aminoacylase-1 OS=Pongo abelii GN=ACY1 PE=2 SV=2)

HSP 1 Score: 134.0 bits (336), Expect = 1.1e-30
Identity = 58/120 (48.33%), Postives = 76/120 (63.33%), Query Frame = -1


Query: 1   PAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFV 60
           P     W+H P  A  DS G IYARG+QDMKC+ +QYLEA+RRLK  G    R+++++FV
Sbjct: 85  PVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCISIQYLEAVRRLKVEGHRFPRTIHMTFV 144

Query: 61  PDEEIGGHGGAEKFAESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKGPWWLVIKAVGAP 120
           PDEE+GGH G E F +  EF  L     LDEG+ +P + + VFY E+ PWW+ + + G P
Sbjct: 145 PDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPTDAFTVFYSERSPWWVRVTSTGRP 204

Query: 121  180
           
Sbjct: 205  204

Query: 181  240
           
Sbjct: 265  204

Query: 241  300
           
Sbjct: 325  204

Query: 301  360
           
Sbjct: 385  204

[Close]

BLAST of WMU43284 vs. Swiss-Prot
Match: ACY1_PIG (Aminoacylase-1 OS=Sus scrofa GN=ACY1 PE=1 SV=2)

HSP 1 Score: 133.3 bits (334), Expect = 1.8e-30
Identity = 59/120 (49.17%), Postives = 75/120 (62.50%), Query Frame = -1


Query: 1   PAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFV 60
           P     W+H P     D+ G IY RG+QDMKCV +QYLEA+RRLK  G    R+++++FV
Sbjct: 85  PVFKEHWSHDPFEGFKDADGYIYGRGAQDMKCVSIQYLEAVRRLKVEGHHFPRTIHMTFV 144

Query: 61  PDEEIGGHGGAEKFAESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKGPWWLVIKAVGAP 120
           PDEE+GGH G E F +  EF+ L     LDEGL SP + + VFY E+ PWWL + + G P
Sbjct: 145 PDEEVGGHQGMELFVKRPEFQALRAGFALDEGLASPTDAFTVFYSERSPWWLRVTSTGKP 204

Query: 121  180
           
Sbjct: 205  204

Query: 181  240
           
Sbjct: 265  204

Query: 241  300
           
Sbjct: 325  204

Query: 301  360
           
Sbjct: 385  204

[Close]

BLAST of WMU43284 vs. Swiss-Prot
Match: ACY1B_RAT (Aminoacylase-1B OS=Rattus norvegicus GN=Acy1b PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 3.1e-30
Identity = 58/120 (48.33%), Postives = 75/120 (62.50%), Query Frame = -1


Query: 1   PAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFV 60
           P     W+H P  A  DS G IY RG+QDMKCV +QYLEA++RLK  G    R+++++FV
Sbjct: 85  PVFKEHWSHDPFEAFKDSEGYIYTRGAQDMKCVSIQYLEAVKRLKVEGHRFPRTIHMTFV 144

Query: 61  PDEEIGGHGGAEKFAESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKGPWWLVIKAVGAP 120
           PDEE+GGH G E F +  EF  L     LDEGL +P + + VFY E+ PWW+ + + G P
Sbjct: 145 PDEEVGGHQGMELFVQRHEFHALRAGFALDEGLANPTDAFTVFYSERSPWWVRVTSTGRP 204

Query: 121  180
           
Sbjct: 205  204

Query: 181  240
           
Sbjct: 265  204

Query: 241  300
           
Sbjct: 325  204

Query: 301  360
           
Sbjct: 385  204

[Close]

BLAST of WMU43284 vs. NCBI nr
Match: gi|659094625|ref|XP_008448158.1| (PREDICTED: aminoacylase-1 [Cucumis melo])

HSP 1 Score: 253.8 bits (647), Expect = 1.5e-64
Identity = 116/120 (96.67%), Postives = 118/120 (98.33%), Query Frame = -1


Query: 1   PAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFV 60
           PAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLK SGF+PLRSVYLSFV
Sbjct: 107 PAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFV 166

Query: 61  PDEEIGGHGGAEKFAESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKGPWWLVIKAVGAP 120
           PDEEIGGHGGAEKFAESDEF+KLNVAIVLDEGLPSPGENYRVFYGEK PWWLVIKAVGAP
Sbjct: 167 PDEEIGGHGGAEKFAESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAP 226

Query: 121  180
           
Sbjct: 227  226

Query: 181  240
           
Sbjct: 287  226

Query: 241  300
           
Sbjct: 347  226

Query: 301  360
           
Sbjct: 407  226

[Close]

BLAST of WMU43284 vs. NCBI nr
Match: gi|449444431|ref|XP_004139978.1| (PREDICTED: aminoacylase-1 [Cucumis sativus])

HSP 1 Score: 250.4 bits (638), Expect = 1.6e-63
Identity = 115/120 (95.83%), Postives = 117/120 (97.50%), Query Frame = -1


Query: 1   PAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFV 60
           PAEH KWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLK SGF+PLRSVYLSFV
Sbjct: 107 PAEHKKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFV 166

Query: 61  PDEEIGGHGGAEKFAESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKGPWWLVIKAVGAP 120
           PDEEIGGHGGAEKFAESDEF+KLNVAIVLDEGLPSPGENYRVFYGEK PWWLVIKAVGAP
Sbjct: 167 PDEEIGGHGGAEKFAESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAP 226

Query: 121  180
           
Sbjct: 227  226

Query: 181  240
           
Sbjct: 287  226

Query: 241  300
           
Sbjct: 347  226

Query: 301  360
           
Sbjct: 407  226

[Close]

BLAST of WMU43284 vs. NCBI nr
Match: gi|731382564|ref|XP_010645690.1| (PREDICTED: aminoacylase-1 isoform X2 [Vitis vinifera])

HSP 1 Score: 221.9 bits (564), Expect = 6.2e-55
Identity = 97/120 (80.83%), Postives = 108/120 (90.00%), Query Frame = -1


Query: 1   PAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFV 60
           PAEHHKWTHPP  AH+D +G+IYARGSQDMKCVG+QYLEAIRRLK SGF+PLR+VYLSFV
Sbjct: 137 PAEHHKWTHPPFDAHVDGNGDIYARGSQDMKCVGLQYLEAIRRLKSSGFQPLRTVYLSFV 196

Query: 61  PDEEIGGHGGAEKFAESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKGPWWLVIKAVGAP 120
           PDEEIGGH GAEKFA+SDEF+K+NV IVLDEGL SP ENYR FY E+ PWWLVI+A GAP
Sbjct: 197 PDEEIGGHDGAEKFADSDEFKKMNVGIVLDEGLASPNENYRAFYAERSPWWLVIRATGAP 256

Query: 121  180
           
Sbjct: 257  256

Query: 181  240
           
Sbjct: 317  256

Query: 241  300
           
Sbjct: 377  256

Query: 301  360
           
Sbjct: 437  256

[Close]

BLAST of WMU43284 vs. NCBI nr
Match: gi|731382562|ref|XP_002283140.2| (PREDICTED: aminoacylase-1 isoform X1 [Vitis vinifera])

HSP 1 Score: 221.9 bits (564), Expect = 6.2e-55
Identity = 97/120 (80.83%), Postives = 108/120 (90.00%), Query Frame = -1


Query: 1   PAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFV 60
           PAEHHKWTHPP  AH+D +G+IYARGSQDMKCVG+QYLEAIRRLK SGF+PLR+VYLSFV
Sbjct: 137 PAEHHKWTHPPFDAHVDGNGDIYARGSQDMKCVGLQYLEAIRRLKSSGFQPLRTVYLSFV 196

Query: 61  PDEEIGGHGGAEKFAESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKGPWWLVIKAVGAP 120
           PDEEIGGH GAEKFA+SDEF+K+NV IVLDEGL SP ENYR FY E+ PWWLVI+A GAP
Sbjct: 197 PDEEIGGHDGAEKFADSDEFKKMNVGIVLDEGLASPNENYRAFYAERSPWWLVIRATGAP 256

Query: 121  180
           
Sbjct: 257  256

Query: 181  240
           
Sbjct: 317  256

Query: 241  300
           
Sbjct: 377  256

Query: 301  360
           
Sbjct: 437  256

[Close]

BLAST of WMU43284 vs. NCBI nr
Match: gi|566188409|ref|XP_002312793.2| (aminoacylase family protein [Populus trichocarpa])

HSP 1 Score: 216.1 bits (549), Expect = 3.4e-53
Identity = 97/120 (80.83%), Postives = 103/120 (85.83%), Query Frame = -1


Query: 1   PAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKESGFEPLRSVYLSFV 60
           P EHHKWTH P GAH+DSHGNI+ARGSQDMKCVGMQYLEAIRRLK SGF PLRSVYLSFV
Sbjct: 107 PVEHHKWTHHPFGAHVDSHGNIFARGSQDMKCVGMQYLEAIRRLKSSGFVPLRSVYLSFV 166

Query: 61  PDEEIGGHGGAEKFAESDEFEKLNVAIVLDEGLPSPGENYRVFYGEKGPWWLVIKAVGAP 120
           PDEEIGGH GA KFA+SD F  +NV IVLDEGL SP ENYR FY E+ PWW+VIKA GAP
Sbjct: 167 PDEEIGGHDGAAKFADSDIFNSMNVGIVLDEGLASPDENYRTFYAERCPWWMVIKATGAP 226

Query: 121  180
           
Sbjct: 227  226

Query: 181  240
           
Sbjct: 287  226

Query: 241  300
           
Sbjct: 347  226

Query: 301  360
           
Sbjct: 407  226

The following BLAST results are available for this feature:

BLAST of WMU43284 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G38220.2	2.1e-53	80.00	Peptidase M20/M25/M40 family protein	[more]
AT1G44180.1	5.0e-39	59.17	Peptidase M20/M25/M40 family protein	[more]
AT1G44820.1	9.5e-38	56.67	Peptidase M20/M25/M40 family protein	[more]

BLAST of WMU43284 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ACY1_DICDI	1.3e-31	54.62	Aminoacylase-1 OS=Dictyostelium discoideum GN=acy1 PE=2 SV=1	[more]
ACY1_HUMAN	8.1e-31	49.17	Aminoacylase-1 OS=Homo sapiens GN=ACY1 PE=1 SV=1	[more]
ACY1_PONAB	1.1e-30	48.33	Aminoacylase-1 OS=Pongo abelii GN=ACY1 PE=2 SV=2	[more]
ACY1_PIG	1.8e-30	49.17	Aminoacylase-1 OS=Sus scrofa GN=ACY1 PE=1 SV=2	[more]
ACY1B_RAT	3.1e-30	48.33	Aminoacylase-1B OS=Rattus norvegicus GN=Acy1b PE=1 SV=1	[more]

BLAST of WMU43284 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659094625\|ref\|XP_008448158.1\|	1.5e-64	96.67	PREDICTED: aminoacylase-1 [Cucumis melo]	[more]
gi\|449444431\|ref\|XP_004139978.1\|	1.6e-63	95.83	PREDICTED: aminoacylase-1 [Cucumis sativus]	[more]
gi\|731382564\|ref\|XP_010645690.1\|	6.2e-55	80.83	PREDICTED: aminoacylase-1 isoform X2 [Vitis vinifera]	[more]
gi\|731382562\|ref\|XP_002283140.2\|	6.2e-55	80.83	PREDICTED: aminoacylase-1 isoform X1 [Vitis vinifera]	[more]
gi\|566188409\|ref\|XP_002312793.2\|	3.4e-53	80.83	aminoacylase family protein [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR001261	ArgE/DapE_CS
IPR002933	Peptidase_M20

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0008237	metallopeptidase activity
GO:0016787	hydrolase activity

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
biological_process	GO:0006508	proteolysis
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0008237	metallopeptidase activity

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Cla001207	Cla001207	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
S4_0136058	S4_0136058	EST
S4_0138869	S4_0138869	EST
S4_0150115	S4_0150115	EST

InterPro

Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001261	ArgE/DapE/ACY1/CPG2/YscS, conserved site	PROSITE	PS00759	ARGE_DAPE_CPG2_2	coord: 27..66 scor
IPR002933	Peptidase M20	PFAM	PF01546	Peptidase_M20	coord: 3..93 score: 6.2
None	No IPR available	GENE3D	G3DSA:3.40.630.10		coord: 1..112 score: 2.1
None	No IPR available	PANTHER	PTHR11014	PEPTIDASE M20 FAMILY MEMBER	coord: 1..120 score: 1.2
None	No IPR available	PANTHER	PTHR11014:SF106	AMINOACYLASE-1	coord: 1..120 score: 1.2
None	No IPR available	unknown	SSF53187	Zn-dependent exopeptidases	coord: 4..114 score: 5.19