WMU32104 (transcribed_cluster) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	WMU32104
Type	transcribed_cluster
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Enoyl-CoA isomerase
Location	Chr5 : 4004242 .. 4005200 (-)
Sequence length	963

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

GTAGAGGTCCCAAAATGTGCACACTCGAGAACCGACGCAATCTCTTCATCCTAACTCTGACCGGCCACGACGACCACCGTCTCAGCCCCTCCTTCATCGCCTCCATCCTCGAAGCTCTTTCCAAGGTCAAGTCTCAAGCCTCCCCAGGCTCCGTTCTCATCACTACTAACCATGGCAAATTCTTCTCGAACGGCTTCGATCTCTCCTGGGCTCAAGCCGCCGGCTCTGTTTCAGCCGCCGCTGACCGCCTCAAGCATATGGTCCAGATTTTCAAGCCCGTCGTCGCCGAACTCCTCTCCCTCCCCATACGCCCACCATCGCCGCCATTTCCGGCCACGCCGCCGCGGCTGGGTTCGTGTTAGCCCTCAGTCACGACTATATGTTCATGAGGAGCGATCGAGGCGTTCTGTACATGAGCGAAGTGGATCTAGGGCTAAGTCTGCCCGATTACTTCATGGCGCTGTTTAAGTCGAAGATCGGCTCGAGTTCCGTCCGCCGCGATGTGTTCCTGAGGGGAATGAAAGTGAAAGGCGAAGCGGCGGTGAGAATGGGGATCGTGGAATCTACGCACGACGGCGAGAACGGCGTCATGGAGGCTGCCGTCCGACTCGGGGAGCAGTTGGGGGCGAGAAATTGGAACGGTGAACCCTACGCGGAAATTAGGAAAAGTCTTTATCCAGAGATCTCTCGGCTGCTTGGATTGCCGGAAAAGGTCATATCGATTTCCAAGCTATGAGAATTGAGAATTGATCGTTCTTTCAGTTGGTATGGTATGTCAATGTGTGCCAATTTATGATGCGAATTTCAAGATTATAATAAATTTCCTGATGCTGTCTGTACTATAAATCGATTGTTTTTCGTCATCTAAAAGTCTGATGAAGAGTTTATGTTCATGGATCGCCGGAAAATTGTTCATCAGGACGCCGGCAGCCAGCTACCGTCTGTGAAAATATCGATCTTAGT

Library


Library category	ESTs
watermelon fruit 10d library	4
watermelon fruit 18d library	2
watermelon fruit 26d library	9
watermelon fruit 34d library	11
Watermelon normalization and subtraction library	1

Homology

[Close]

BLAST of WMU32104 vs. TAIR10
Match: AT4G14430.1 (AT4G14430.1 indole-3-butyric acid response 10)

HSP 1 Score: 161.0 bits (406), Expect = 1.2e-39
Identity = 78/127 (61.42%), Postives = 100/127 (78.74%), Query Frame = 2


Query: 356 VLALSHDYMFMRSDRGVLYMSEVDLGLSLPDYFMALFKSKIGSSSVRRDVFLRGMKVKGE 415
           +LALSHDY+FMR DRGVLYMSEVD+GLS+PDYF AL ++KIG+S+ RR++ L G K++GE
Sbjct: 114 ILALSHDYVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGE 173

Query: 416 AAVRMGIVES-THDGENGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLLGLPEK 475
            AV +GIV+S  +D E GV+ A+VRLGE+L A+ W+GE YA IRKSLYPE+  +LGL  +
Sbjct: 174 EAVGLGIVDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETR 233

Query: 476 VISISKL 535
           V +  KL
Sbjct: 234 VFATPKL 240

Query: 536  595
           
Sbjct: 294  240

Query: 596  655
           
Sbjct: 354  240

Query: 656  715
           
Sbjct: 414  240

Query: 716  734
           
Sbjct: 474  240

HSP 2 Score: 97.1 bits (240), Expect = 2.2e-20
Identity = 50/91 (54.95%), Postives = 59/91 (64.84%), Query Frame = 3


Query: 15  MCTLENRRNLFILTLTGHDDHRLSPSFIASILEALSKVKSQASPGSVLITTNHGKFFSNG 74
           MCTLE R +LF+LTLTG  +HR  P  IA+IL  L + KSQ++ GS+LITT +GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 75  FDLXXXXXXXXXXXXXDRLKHMVQIFKPVVA 134
           FDL             +RL  MV+ FKPVVA
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVA 91

Query: 135  194
           
Sbjct: 121  91

Query: 195  254
           
Sbjct: 181  91

Query: 255  288
           
Sbjct: 241  91

[Close]

BLAST of WMU32104 vs. TAIR10
Match: AT4G14440.1 (AT4G14440.1 3-hydroxyacyl-CoA dehydratase 1)

HSP 1 Score: 149.1 bits (375), Expect = 4.8e-36
Identity = 73/117 (62.39%), Postives = 87/117 (74.36%), Query Frame = 2


Query: 356 VLALSHDYMFMRSDRGVLYMSEVDLGLSLPDYFMALFKSKIGSSSVRRDVFLRGMKVKGE 415
           + ALSHDY+FMR DRGVLYMSEVD+GL +PDYF AL  +K+GS   RR++ L G K+KGE
Sbjct: 112 MFALSHDYVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGE 171

Query: 416 AAVRMGIVES-THDGENGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLLGL 475
            AV +GIV+S  HD   GV+EA V LGE L A+ WNGE YA IRKSLYPE+ R++ L
Sbjct: 172 EAVALGIVDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDL 228

Query: 476  535
           
Sbjct: 232  228

Query: 536  595
           
Sbjct: 292  228

Query: 596  655
           
Sbjct: 352  228

Query: 656  704
           
Sbjct: 412  228

HSP 2 Score: 97.8 bits (242), Expect = 1.3e-20
Identity = 51/91 (56.04%), Postives = 59/91 (64.84%), Query Frame = 3


Query: 15  MCTLENRRNLFILTLTGHDDHRLSPSFIASILEALSKVKSQASPGSVLITTNHGKFFSNG 74
           MCTLE R +LF+LTLTG D+HR  P  IAS+L  L + KSQ++ GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 75  FDLXXXXXXXXXXXXXDRLKHMVQIFKPVVA 134
           FDL              R+  MV+ FKPV+A
Sbjct: 61  FDL--AWAQSAGHGAIKRMHQMVKSFKPVLA 89

Query: 135  194
           
Sbjct: 121  89

Query: 195  254
           
Sbjct: 181  89

Query: 255  288
           
Sbjct: 241  89

[Close]

BLAST of WMU32104 vs. TAIR10
Match: AT1G65520.1 (AT1G65520.1 delta(3), delta(2)-enoyl CoA isomerase 1)

HSP 1 Score: 109.4 bits (272), Expect = 4.2e-24
Identity = 52/110 (47.27%), Postives = 74/110 (67.27%), Query Frame = 2


Query: 356 VLALSHDYMFMRSDRGVLYMSEVDLGLSLPDYFMALFKSKIGSSSVRRDVFLRGMKVKGE 415
           +LA+SHDY+ MR DRG LYMSE+D+ L +P +FMA+ + KIGS + RRDV L   KV  +
Sbjct: 109 ILAMSHDYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTAD 168

Query: 416 AAVRMGIVESTHDGENGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEI 475
             V+MGIV+S +      +EAA++LGE++  R  +G  Y ++R+SL  E+
Sbjct: 169 VGVKMGIVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

Query: 476  535
           
Sbjct: 229  218

Query: 536  595
           
Sbjct: 289  218

Query: 596  655
           
Sbjct: 349  218

Query: 656  686
           
Sbjct: 409  218

HSP 2 Score: 69.3 bits (168), Expect = 4.8e-12
Identity = 34/64 (53.12%), Postives = 45/64 (70.31%), Query Frame = 3


Query: 15  MCTLENRRNLFILTLTGHDDHRLSPSFIASILEALSKVKSQAS-PGSVLITTNHGKFFSN 74
           MC+LE R  LFIL LTG  +HRL+P+ + S+   +++++S  S   SVLITT+ GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 75  GFDL 134
           G+DL
Sbjct: 61  GYDL 64

Query: 135  194
           
Sbjct: 121  64

Query: 195  204
           
Sbjct: 181  64

[Close]

BLAST of WMU32104 vs. Swiss-Prot
Match: ECI2_ARATH (Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana GN=ECI2 PE=1 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.2e-38
Identity = 78/127 (61.42%), Postives = 100/127 (78.74%), Query Frame = 2


Query: 356 VLALSHDYMFMRSDRGVLYMSEVDLGLSLPDYFMALFKSKIGSSSVRRDVFLRGMKVKGE 415
           +LALSHDY+FMR DRGVLYMSEVD+GLS+PDYF AL ++KIG+S+ RR++ L G K++GE
Sbjct: 114 ILALSHDYVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGE 173

Query: 416 AAVRMGIVES-THDGENGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLLGLPEK 475
            AV +GIV+S  +D E GV+ A+VRLGE+L A+ W+GE YA IRKSLYPE+  +LGL  +
Sbjct: 174 EAVGLGIVDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETR 233

Query: 476 VISISKL 535
           V +  KL
Sbjct: 234 VFATPKL 240

Query: 536  595
           
Sbjct: 294  240

Query: 596  655
           
Sbjct: 354  240

Query: 656  715
           
Sbjct: 414  240

Query: 716  734
           
Sbjct: 474  240

HSP 2 Score: 97.1 bits (240), Expect = 3.8e-19
Identity = 50/91 (54.95%), Postives = 59/91 (64.84%), Query Frame = 3


Query: 15  MCTLENRRNLFILTLTGHDDHRLSPSFIASILEALSKVKSQASPGSVLITTNHGKFFSNG 74
           MCTLE R +LF+LTLTG  +HR  P  IA+IL  L + KSQ++ GS+LITT +GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 75  FDLXXXXXXXXXXXXXDRLKHMVQIFKPVVA 134
           FDL             +RL  MV+ FKPVVA
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVA 91

Query: 135  194
           
Sbjct: 121  91

Query: 195  254
           
Sbjct: 181  91

Query: 255  288
           
Sbjct: 241  91

[Close]

BLAST of WMU32104 vs. Swiss-Prot
Match: ECI3_ARATH (Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana GN=ECI3 PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 8.5e-35
Identity = 73/117 (62.39%), Postives = 87/117 (74.36%), Query Frame = 2


Query: 356 VLALSHDYMFMRSDRGVLYMSEVDLGLSLPDYFMALFKSKIGSSSVRRDVFLRGMKVKGE 415
           + ALSHDY+FMR DRGVLYMSEVD+GL +PDYF AL  +K+GS   RR++ L G K+KGE
Sbjct: 112 MFALSHDYVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGE 171

Query: 416 AAVRMGIVES-THDGENGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLLGL 475
            AV +GIV+S  HD   GV+EA V LGE L A+ WNGE YA IRKSLYPE+ R++ L
Sbjct: 172 EAVALGIVDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDL 228

Query: 476  535
           
Sbjct: 232  228

Query: 536  595
           
Sbjct: 292  228

Query: 596  655
           
Sbjct: 352  228

Query: 656  704
           
Sbjct: 412  228

HSP 2 Score: 97.8 bits (242), Expect = 2.2e-19
Identity = 51/91 (56.04%), Postives = 59/91 (64.84%), Query Frame = 3


Query: 15  MCTLENRRNLFILTLTGHDDHRLSPSFIASILEALSKVKSQASPGSVLITTNHGKFFSNG 74
           MCTLE R +LF+LTLTG D+HR  P  IAS+L  L + KSQ++ GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 75  FDLXXXXXXXXXXXXXDRLKHMVQIFKPVVA 134
           FDL              R+  MV+ FKPV+A
Sbjct: 61  FDL--AWAQSAGHGAIKRMHQMVKSFKPVLA 89

Query: 135  194
           
Sbjct: 121  89

Query: 195  254
           
Sbjct: 181  89

Query: 255  288
           
Sbjct: 241  89

[Close]

BLAST of WMU32104 vs. Swiss-Prot
Match: ECI1_ARATH (Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana GN=ECI1 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 7.5e-23
Identity = 52/110 (47.27%), Postives = 74/110 (67.27%), Query Frame = 2


Query: 356 VLALSHDYMFMRSDRGVLYMSEVDLGLSLPDYFMALFKSKIGSSSVRRDVFLRGMKVKGE 415
           +LA+SHDY+ MR DRG LYMSE+D+ L +P +FMA+ + KIGS + RRDV L   KV  +
Sbjct: 109 ILAMSHDYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTAD 168

Query: 416 AAVRMGIVESTHDGENGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEI 475
             V+MGIV+S +      +EAA++LGE++  R  +G  Y ++R+SL  E+
Sbjct: 169 VGVKMGIVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

Query: 476  535
           
Sbjct: 229  218

Query: 536  595
           
Sbjct: 289  218

Query: 596  655
           
Sbjct: 349  218

Query: 656  686
           
Sbjct: 409  218

HSP 2 Score: 69.3 bits (168), Expect = 8.5e-11
Identity = 34/64 (53.12%), Postives = 45/64 (70.31%), Query Frame = 3


Query: 15  MCTLENRRNLFILTLTGHDDHRLSPSFIASILEALSKVKSQAS-PGSVLITTNHGKFFSN 74
           MC+LE R  LFIL LTG  +HRL+P+ + S+   +++++S  S   SVLITT+ GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 75  GFDL 134
           G+DL
Sbjct: 61  GYDL 64

Query: 135  194
           
Sbjct: 121  64

Query: 195  204
           
Sbjct: 181  64

[Close]

BLAST of WMU32104 vs. TrEMBL
Match: A0A0A0L8C7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141780 PE=4 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 4.7e-56
Identity = 113/127 (88.98%), Postives = 118/127 (92.91%), Query Frame = 2


Query: 353 FVLALSHDYMFMRSDRGVLYMSEVDLGLSLPDYFMALFKSKIGSSSVRRDVFLRGMKVKG 412
           F+LALSHDY+ MRSDRGVLYMSEVDLGLSLPDYF ALFKSKI SSSVRRDV +RGMKVKG
Sbjct: 113 FLLALSHDYLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIASSSVRRDVLMRGMKVKG 172

Query: 413 EAAVRMGIVESTHDGENGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLLGLPEK 472
           EAAV+MGI +S HDGENGVMEAAVRLGEQLG R WNGEPYAEIRKSLYPEISRLLGLPEK
Sbjct: 173 EAAVKMGIADSAHDGENGVMEAAVRLGEQLGERKWNGEPYAEIRKSLYPEISRLLGLPEK 232

Query: 473 VISISKL 532
            ISISKL
Sbjct: 233 AISISKL 239

Query: 533  592
           
Sbjct: 293  239

Query: 593  652
           
Sbjct: 353  239

Query: 653  712
           
Sbjct: 413  239

Query: 713  734
           
Sbjct: 473  239

[Close]

BLAST of WMU32104 vs. TrEMBL
Match: A0A0A0L8C7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141780 PE=4 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.7e-29
Identity = 71/92 (77.17%), Postives = 74/92 (80.43%), Query Frame = 3


Query: 15  MCTLENRRNLFILTLTGHDDHRLSPSFIASILEALSKVKSQASPGSVLITTNHGKFFSNG 74
           MCTLE RRNLFILTLTGHDDHR SPS IA+ILEALSKVKSQAS GSVLITT+HGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGHDDHRFSPSVIAAILEALSKVKSQASTGSVLITTSHGKFFSNG 60

Query: 75  FDLXXXXXXXXXXXXXDRLKHMVQIFKPVVAE 134
           FDL             +RL HMVQIFKPVVAE
Sbjct: 61  FDLSWAQAAGSVSAAAERLHHMVQIFKPVVAE 92

Query: 135  194
           
Sbjct: 121  92

Query: 195  254
           
Sbjct: 181  92

Query: 255  291
           
Sbjct: 241  92

HSP 2 Score: 202.6 bits (514), Expect = 7.2e-49
Identity = 101/126 (80.16%), Postives = 114/126 (90.48%), Query Frame = 2


Query: 356 VLALSHDYMFMRSDRGVLYMSEVDLGLSLPDYFMALFKSKIGSSSVRRDVFLRGMKVKGE 415
           VLAL+HDY+ MRSDRGVLYMSE+DLG +LPDYF+AL KSKIGSSSVRRDVFLRGMKV+GE
Sbjct: 33  VLALAHDYLVMRSDRGVLYMSELDLGATLPDYFIALAKSKIGSSSVRRDVFLRGMKVRGE 92

Query: 416 AAVRMGIVESTHDGENGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLLGLPEKV 475
            AV+MG+ ES H GE+GVMEAAVRLGE+L ARNW+G+ YAEIRKSLYPEIS LLGL  KV
Sbjct: 93  TAVKMGLAESAHQGEDGVMEAAVRLGEELAARNWDGDVYAEIRKSLYPEISGLLGLTTKV 152

Query: 476 ISISKL 535
           I+ISKL
Sbjct: 153 ITISKL 158

Query: 536  595
           
Sbjct: 213  158

Query: 596  655
           
Sbjct: 273  158

Query: 656  715
           
Sbjct: 333  158

Query: 716  734
           
Sbjct: 393  158

[Close]

BLAST of WMU32104 vs. TrEMBL
Match: A0A0A0L9X4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141770 PE=4 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.2e-48
Identity = 100/127 (78.74%), Postives = 111/127 (87.40%), Query Frame = 2


Query: 353 FVLALSHDYMFMRSDRGVLYMSEVDLGLSLPDYFMALFKSKIGSSSVRRDVFLRGMKVKG 412
           F LALSHDY+ MRSDRGVLYM E+DLGL+LPDYFMALFKSKIGSS   RD+ LRGMKV+G
Sbjct: 113 FTLALSHDYLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRG 172

Query: 413 EAAVRMGIVESTHDGENGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLLGLPEK 472
           EAAVRMGIV+S HDGE+ V+ AAVRLGEQL  RNW+GE YAEIRKSLYPE+S +LGLP K
Sbjct: 173 EAAVRMGIVDSAHDGEDSVVNAAVRLGEQLAGRNWDGEVYAEIRKSLYPEVSEVLGLPGK 232

Query: 473 VISISKL 532
            ISISKL
Sbjct: 233 PISISKL 239

Query: 533  592
           
Sbjct: 293  239

Query: 593  652
           
Sbjct: 353  239

Query: 653  712
           
Sbjct: 413  239

Query: 713  734
           
Sbjct: 473  239

[Close]

BLAST of WMU32104 vs. TrEMBL
Match: A0A0A0L9X4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141770 PE=4 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 4.7e-24
Identity = 61/92 (66.30%), Postives = 69/92 (75.00%), Query Frame = 3


Query: 15  MCTLENRRNLFILTLTGHDDHRLSPSFIASILEALSKVKSQASPGSVLITTNHGKFFSNG 74
           MCT+E R N FILTLTG DDHRLSPS I SIL+ALS+VK+QASP SVLITT+HGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 75  FDLXXXXXXXXXXXXXDRLKHMVQIFKPVVAE 134
           FDL              RL H+++IFK VVAE
Sbjct: 61  FDLPWALSAGSVSAARHRLNHLIEIFKSVVAE 92

Query: 135  194
           
Sbjct: 121  92

Query: 195  254
           
Sbjct: 181  92

Query: 255  291
           
Sbjct: 241  92

HSP 2 Score: 168.3 bits (425), Expect = 1.5e-38
Identity = 81/127 (63.78%), Postives = 103/127 (81.10%), Query Frame = 2


Query: 356 VLALSHDYMFMRSDRGVLYMSEVDLGLSLPDYFMALFKSKIGSSSVRRDVFLRGMKVKGE 415
           +LALSHDY+FMR+DRGVLYMSEVD+GLS+PDYF A+ +SKIG+S+ RR+VFL G K++GE
Sbjct: 161 MLALSHDYVFMRNDRGVLYMSEVDIGLSMPDYFAAIVRSKIGTSAARREVFLSGKKIRGE 220

Query: 416 AAVRMGIVES-THDGENGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLLGLPEK 475
            AV +GIV+S  HD E GV+EA VRLGE+L A+ W+GE YA IRKSLYPE+  +L L  K
Sbjct: 221 EAVALGIVDSAAHDSEEGVLEATVRLGEKLAAKKWSGEVYASIRKSLYPELCGMLNLEAK 280

Query: 476 VISISKL 535
           + +  KL
Sbjct: 281 IFATPKL 287

Query: 536  595
           
Sbjct: 341  287

Query: 596  655
           
Sbjct: 401  287

Query: 656  715
           
Sbjct: 461  287

Query: 716  734
           
Sbjct: 521  287

[Close]

BLAST of WMU32104 vs. TrEMBL
Match: V4MKX3_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10027007mg PE=4 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 2.3e-18
Identity = 52/91 (57.14%), Postives = 62/91 (68.13%), Query Frame = 3


Query: 15  MCTLENRRNLFILTLTGHDDHRLSPSFIASILEALSKVKSQASPGSVLITTNHGKFFSNG 74
           MCTLE R +LF+LTLTG  +HR  P+ IASI+  L + KSQ+  GSVLITT +GKFFSNG
Sbjct: 48  MCTLEKRGDLFLLTLTGDGEHRFHPNTIASIISLLEQAKSQSKRGSVLITTANGKFFSNG 107

Query: 75  FDLXXXXXXXXXXXXXDRLKHMVQIFKPVVA 134
           FDL             +RL HMV+ FKPVVA
Sbjct: 108 FDLAWAQSSGSKTAAAERLHHMVESFKPVVA 138

Query: 135  194
           
Sbjct: 168  138

Query: 195  254
           
Sbjct: 228  138

Query: 255  288
           
Sbjct: 288  138

HSP 2 Score: 166.4 bits (420), Expect = 5.7e-38
Identity = 79/127 (62.20%), Postives = 100/127 (78.74%), Query Frame = 2


Query: 353 FVLALSHDYMFMRSDRGVLYMSEVDLGLSLPDYFMALFKSKIGSSSVRRDVFLRGMKVKG 412
           F LALSHDY  MR DRGVLYM+EVD+GLS+PDYFMALFK K+G++S RRD+ LRG+K+KG
Sbjct: 113 FALALSHDYAVMRRDRGVLYMAEVDIGLSVPDYFMALFKEKMGAASARRDLLLRGLKMKG 172

Query: 413 EAAVRMGIVESTHDGENGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLLGLPEK 472
           + A + GIVE+ +D +  V EA+VR+GE L  R W+GE YAEIRK LYPE+S +LGL  K
Sbjct: 173 DEAEKRGIVEAAYDSDGEVREASVRMGEDLAKRKWDGEVYAEIRKGLYPELSAMLGLATK 232

Query: 473 VISISKL 532
           V +  +L
Sbjct: 233 VFASPRL 239

Query: 533  592
           
Sbjct: 293  239

Query: 593  652
           
Sbjct: 353  239

Query: 653  712
           
Sbjct: 413  239

Query: 713  734
           
Sbjct: 473  239

[Close]

BLAST of WMU32104 vs. NCBI nr
Match: gi|449432586|ref|XP_004134080.1| (PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis sativus])

HSP 1 Score: 226.9 bits (577), Expect = 5.1e-56
Identity = 113/127 (88.98%), Postives = 118/127 (92.91%), Query Frame = 2


Query: 353 FVLALSHDYMFMRSDRGVLYMSEVDLGLSLPDYFMALFKSKIGSSSVRRDVFLRGMKVKG 412
           F+LALSHDY+ MRSDRGVLYMSEVDLGLSLPDYF ALFKSKI SSSVRRDV +RGMKVKG
Sbjct: 113 FLLALSHDYLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIASSSVRRDVLMRGMKVKG 172

Query: 413 EAAVRMGIVESTHDGENGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLLGLPEK 472
           EAAV+MGI +S HDGENGVMEAAVRLGEQLG R WNGEPYAEIRKSLYPEISRLLGLPEK
Sbjct: 173 EAAVKMGIADSAHDGENGVMEAAVRLGEQLGERKWNGEPYAEIRKSLYPEISRLLGLPEK 232

Query: 473 VISISKL 532
            ISISKL
Sbjct: 233 AISISKL 239

Query: 533  592
           
Sbjct: 293  239

Query: 593  652
           
Sbjct: 353  239

Query: 653  712
           
Sbjct: 413  239

Query: 713  734
           
Sbjct: 473  239

[Close]

BLAST of WMU32104 vs. NCBI nr
Match: gi|659076208|ref|XP_008438558.1| (PREDICTED: enoyl-CoA hydratase domain-containing protein 2, mitochondrial-like [Cucumis melo])

HSP 1 Score: 226.9 bits (577), Expect = 5.1e-56
Identity = 113/127 (88.98%), Postives = 120/127 (94.49%), Query Frame = 2


Query: 353 FVLALSHDYMFMRSDRGVLYMSEVDLGLSLPDYFMALFKSKIGSSSVRRDVFLRGMKVKG 412
           F+LALSHDY+ MRSDRGVLYMSEVDLGLSLPDYF ALFKSKIGSSSVRRDV +RGMKVKG
Sbjct: 113 FMLALSHDYLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIGSSSVRRDVLMRGMKVKG 172

Query: 413 EAAVRMGIVESTHDGENGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLLGLPEK 472
           EAAV+MGI +S +DGE+GVMEAAVRLGEQLGAR WNGEPYAEIRKSLYPEISRLLGLPEK
Sbjct: 173 EAAVKMGIADSAYDGESGVMEAAVRLGEQLGARKWNGEPYAEIRKSLYPEISRLLGLPEK 232

Query: 473 VISISKL 532
            ISISKL
Sbjct: 233 AISISKL 239

Query: 533  592
           
Sbjct: 293  239

Query: 593  652
           
Sbjct: 353  239

Query: 653  712
           
Sbjct: 413  239

Query: 713  734
           
Sbjct: 473  239

[Close]

BLAST of WMU32104 vs. NCBI nr
Match: gi|700201750|gb|KGN56883.1| (hypothetical protein Csa_3G141790 [Cucumis sativus])

HSP 1 Score: 203.0 bits (515), Expect = 7.9e-49
Identity = 101/126 (80.16%), Postives = 114/126 (90.48%), Query Frame = 2


Query: 356 VLALSHDYMFMRSDRGVLYMSEVDLGLSLPDYFMALFKSKIGSSSVRRDVFLRGMKVKGE 415
           VLAL+HDY+ MRSDRGVLYMSE+DLG +LPDYF+AL KSKIGSSSVRRDVFLRGMKV+GE
Sbjct: 33  VLALAHDYLVMRSDRGVLYMSELDLGATLPDYFIALAKSKIGSSSVRRDVFLRGMKVRGE 92

Query: 416 AAVRMGIVESTHDGENGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLLGLPEKV 475
            AV+MG+ ES H GE+GVMEAAVRLGE+L ARNW+G+ YAEIRKSLYPEIS LLGL  KV
Sbjct: 93  TAVKMGLAESAHQGEDGVMEAAVRLGEELAARNWDGDVYAEIRKSLYPEISGLLGLTTKV 152

Query: 476 ISISKL 535
           I+ISKL
Sbjct: 153 ITISKL 158

Query: 536  595
           
Sbjct: 213  158

Query: 596  655
           
Sbjct: 273  158

Query: 656  715
           
Sbjct: 333  158

Query: 716  734
           
Sbjct: 393  158

[Close]

BLAST of WMU32104 vs. NCBI nr
Match: gi|449432588|ref|XP_004134081.1| (PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis sativus])

HSP 1 Score: 203.0 bits (515), Expect = 7.9e-49
Identity = 101/126 (80.16%), Postives = 114/126 (90.48%), Query Frame = 2


Query: 356 VLALSHDYMFMRSDRGVLYMSEVDLGLSLPDYFMALFKSKIGSSSVRRDVFLRGMKVKGE 415
           VLAL+HDY+ MRSDRGVLYMSE+DLG +LPDYF+AL KSKIGSSSVRRDVFLRGMKV+GE
Sbjct: 116 VLALAHDYLVMRSDRGVLYMSELDLGATLPDYFIALAKSKIGSSSVRRDVFLRGMKVRGE 175

Query: 416 AAVRMGIVESTHDGENGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLLGLPEKV 475
            AV+MG+ ES H GE+GVMEAAVRLGE+L ARNW+G+ YAEIRKSLYPEIS LLGL  KV
Sbjct: 176 TAVKMGLAESAHQGEDGVMEAAVRLGEELAARNWDGDVYAEIRKSLYPEISGLLGLTTKV 235

Query: 476 ISISKL 535
           I+ISKL
Sbjct: 236 ITISKL 241

Query: 536  595
           
Sbjct: 296  241

Query: 596  655
           
Sbjct: 356  241

Query: 656  715
           
Sbjct: 416  241

Query: 716  734
           
Sbjct: 476  241

[Close]

BLAST of WMU32104 vs. NCBI nr
Match: gi|449432584|ref|XP_004134079.1| (PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis sativus])

HSP 1 Score: 202.2 bits (513), Expect = 1.4e-48
Identity = 100/127 (78.74%), Postives = 111/127 (87.40%), Query Frame = 2


Query: 353 FVLALSHDYMFMRSDRGVLYMSEVDLGLSLPDYFMALFKSKIGSSSVRRDVFLRGMKVKG 412
           F LALSHDY+ MRSDRGVLYM E+DLGL+LPDYFMALFKSKIGSS   RD+ LRGMKV+G
Sbjct: 113 FTLALSHDYLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRG 172

Query: 413 EAAVRMGIVESTHDGENGVMEAAVRLGEQLGARNWNGEPYAEIRKSLYPEISRLLGLPEK 472
           EAAVRMGIV+S HDGE+ V+ AAVRLGEQL  RNW+GE YAEIRKSLYPE+S +LGLP K
Sbjct: 173 EAAVRMGIVDSAHDGEDSVVNAAVRLGEQLAGRNWDGEVYAEIRKSLYPEVSEVLGLPGK 232

Query: 473 VISISKL 532
            ISISKL
Sbjct: 233 PISISKL 239

Query: 533  592
           
Sbjct: 293  239

Query: 593  652
           
Sbjct: 353  239

Query: 653  712
           
Sbjct: 413  239

Query: 713  734
           
Sbjct: 473  239

The following BLAST results are available for this feature:

BLAST of WMU32104 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G14430.1	1.2e-39	61.42	indole-3-butyric acid response 10	[more]
AT4G14440.1	4.8e-36	62.39	3-hydroxyacyl-CoA dehydratase 1	[more]
AT1G65520.1	4.2e-24	47.27	delta(3), delta(2)-enoyl CoA isomerase 1	[more]

BLAST of WMU32104 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ECI2_ARATH	2.2e-38	61.42	Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana GN=ECI2 PE=1 SV...	[more]
ECI3_ARATH	8.5e-35	62.39	Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana GN=ECI3 PE=1 SV=1	[more]
ECI1_ARATH	7.5e-23	47.27	Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana GN=ECI1 PE=1 SV...	[more]

BLAST of WMU32104 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8C7_CUCSA	4.7e-56	88.98	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141780 PE=4 SV=1	[more]
A0A0A0L8C7_CUCSA	1.7e-29	77.17	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141780 PE=4 SV=1	[more]
A0A0A0L9X4_CUCSA	1.2e-48	78.74	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141770 PE=4 SV=1	[more]
A0A0A0L9X4_CUCSA	4.7e-24	66.30	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G141770 PE=4 SV=1	[more]
V4MKX3_EUTSA	2.3e-18	57.14	Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10027007mg PE=4 SV=1	[more]

BLAST of WMU32104 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449432586\|ref\|XP_004134080.1\|	5.1e-56	88.98	PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis...	[more]
gi\|659076208\|ref\|XP_008438558.1\|	5.1e-56	88.98	PREDICTED: enoyl-CoA hydratase domain-containing protein 2, mitochondrial-like [...	[more]
gi\|700201750\|gb\|KGN56883.1\|	7.9e-49	80.16	hypothetical protein Csa_3G141790 [Cucumis sativus]	[more]
gi\|449432588\|ref\|XP_004134081.1\|	7.9e-49	80.16	PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis...	[more]
gi\|449432584\|ref\|XP_004134079.1\|	1.4e-48	78.74	PREDICTED: 3-hydroxybutyryl-CoA dehydratase-like protein, mitochondrial [Cucumis...	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR001753	Enoyl-CoA_hydra/iso

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
molecular_function	GO:0003824	catalytic activity

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Cla021543	Cla021543	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
S1_0044794	S1_0044794	EST
S1_0078838	S1_0078838	EST
S1_0082695	S1_0082695	EST
S1_0131490	S1_0131490	EST
S2_0106480	S2_0106480	EST
S2_0176130	S2_0176130	EST
S3_0032250	S3_0032250	EST
S3_0036188	S3_0036188	EST
S3_0049151	S3_0049151	EST
S3_0073360	S3_0073360	EST
S3_0098287	S3_0098287	EST
S3_0123740	S3_0123740	EST
S3_0136934	S3_0136934	EST
S3_0139242	S3_0139242	EST
S3_0177343	S3_0177343	EST
S4_0034829	S4_0034829	EST
S4_0040128	S4_0040128	EST
S4_0040296	S4_0040296	EST
S4_0049468	S4_0049468	EST
S4_0095102	S4_0095102	EST
S4_0143514	S4_0143514	EST
S4_0148581	S4_0148581	EST
S4_0153282	S4_0153282	EST
S4_0155631	S4_0155631	EST
S4_0160075	S4_0160075	EST
S4_0174422	S4_0174422	EST
WMN29E12	WMN29E12	EST

InterPro

Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001753	Crotonase superfamily	PFAM	PF00378	ECH_1	coord: 28..209 score: 1.8
None	No IPR available	PANTHER	PTHR11941	ENOYL-COA HYDRATASE-RELATED	coord: 88..229 score: 3.5E-86coord: 6..72 score: 3.5
None	No IPR available	PANTHER	PTHR11941:SF84	SUBFAMILY NOT NAMED	coord: 88..229 score: 3.5E-86coord: 6..72 score: 3.5