WMU14543 (transcribed_cluster) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	WMU14543
Type	transcribed_cluster
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Metal tolerance protein
Location	Chr6 : 810360 .. 815064 (-)
Sequence length	398

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

TTCTGCCTTTTGCTAGGGCTTTCTTCACCAACTTCAGCTTTCTTCTCCTCCAACCGAGATGGAAGTGCAAGATCATGGTCACATAATTGAAGTATGTGGAGATGTGCAAGCTGTAGGGCCTAGCACTGTGGGAAGTAAAATTTGTGGGGATGCACCCTCTTGTGGATTTTCTGATGCAAAAAGCAGTTCAAAAGATGCAAAGGAGCGATCTGCGTCTATGCGGAAGCTTTGCATAGCAGTTGTTCTCTGCATCGTTTTATGAGTGTAGAAGTAGTCGGAGGTATTAAAGCAAACAGTCTTGCAATATTGACTGATGCTGCTCATTTGTTGTCAGATGTTGCTGCTTTTGCCATTTCTTATTCTCACTCTGGGCCTCTGGTTGGGAGGCAACTCCACGT

Library


Library category	ESTs
watermelon fruit 26d library	4

Homology

[Close]

BLAST of WMU14543 vs. TAIR10
Match: AT2G46800.1 (AT2G46800.1 zinc transporter of Arabidopsis thaliana)

HSP 1 Score: 65.9 bits (159), Expect = 2.2e-11
Identity = 40/73 (54.79%), Postives = 44/73 (60.27%), Query Frame = 1


Query: 190 KRCKGAICVYAEALHSSCSLHR-----------------FMSVEVVGGIKANSLAILTDA 249
           K C  A C ++++ ++S   H                  FMSVEVVGGIKANSLAILTDA
Sbjct: 29  KVCGEAPCGFSDSKNASGDAHERSASMRKLCIAVVLCLVFMSVEVVGGIKANSLAILTDA 88

Query: 250 AHLLSDVAAFAIS 309
           AHLLSDVAAFAIS
Sbjct: 89  AHLLSDVAAFAIS 101

Query: 310  358
           
Sbjct: 149  101

[Close]

BLAST of WMU14543 vs. TAIR10
Match: AT3G58810.1 (AT3G58810.1 metal tolerance protein A2)

HSP 1 Score: 61.6 bits (148), Expect = 4.1e-10
Identity = 32/34 (94.12%), Postives = 31/34 (91.18%), Query Frame = 1


Query: 256 FMSVEVVGGIKANSLAILTDAAHLLSDVAAFAIS 315
           F+ VEVVGGIKANSLAILTDAAHLLSDVAAFAIS
Sbjct: 123 FIVVEVVGGIKANSLAILTDAAHLLSDVAAFAIS 156

Query: 316  358
           
Sbjct: 183  156

[Close]

BLAST of WMU14543 vs. TAIR10
Match: AT3G61940.1 (AT3G61940.1 Cation efflux family protein)

HSP 1 Score: 57.0 bits (136), Expect = 1.0e-08
Identity = 29/34 (85.29%), Postives = 29/34 (85.29%), Query Frame = 1


Query: 256 FMSVEVVGGIKANSLAILTDAAHLLSDVAAFAIS 315
           FMS+EVV GIKANSLAIL DAAHLL+DV AFAIS
Sbjct: 43  FMSIEVVCGIKANSLAILADAAHLLTDVGAFAIS 76

Query: 316  358
           
Sbjct: 103  76

[Close]

BLAST of WMU14543 vs. TAIR10
Match: AT2G29410.1 (AT2G29410.1 metal tolerance protein B1)

HSP 1 Score: 53.1 bits (126), Expect = 1.5e-07
Identity = 24/33 (72.73%), Postives = 27/33 (81.82%), Query Frame = 1


Query: 259 MSVEVVGGIKANSLAILTDAAHLLSDVAAFAIS 318
           MSV++VGG KANSLA++TDAAHLLSDVA   +S
Sbjct: 69  MSVQIVGGFKANSLAVMTDAAHLLSDVAGLCVS 101

Query: 319  358
           
Sbjct: 129  101

[Close]

BLAST of WMU14543 vs. Swiss-Prot
Match: MTP1_ORYSJ (Metal tolerance protein 1 OS=Oryza sativa subsp. japonica GN=MTP1 PE=2 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 6.0e-11
Identity = 41/73 (56.16%), Postives = 48/73 (65.75%), Query Frame = 1


Query: 190 KRCKGAICVYAEALHSS-------CSLHR----------FMSVEVVGGIKANSLAILTDA 249
           K C+GA C ++++ +SS        S+ +          FM+VEVVGGIKANSLAILTDA
Sbjct: 29  KVCRGAACDFSDSSNSSKDARERMASMRKLIIAVILCIIFMAVEVVGGIKANSLAILTDA 88

Query: 250 AHLLSDVAAFAIS 309
           AHLLSDVAAFAIS
Sbjct: 89  AHLLSDVAAFAIS 101

Query: 310  358
           
Sbjct: 149  101

[Close]

BLAST of WMU14543 vs. Swiss-Prot
Match: MTP1_ARATH (Metal tolerance protein 1 OS=Arabidopsis thaliana GN=MTP1 PE=1 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 3.9e-10
Identity = 40/73 (54.79%), Postives = 44/73 (60.27%), Query Frame = 1


Query: 190 KRCKGAICVYAEALHSSCSLHR-----------------FMSVEVVGGIKANSLAILTDA 249
           K C  A C ++++ ++S   H                  FMSVEVVGGIKANSLAILTDA
Sbjct: 29  KVCGEAPCGFSDSKNASGDAHERSASMRKLCIAVVLCLVFMSVEVVGGIKANSLAILTDA 88

Query: 250 AHLLSDVAAFAIS 309
           AHLLSDVAAFAIS
Sbjct: 89  AHLLSDVAAFAIS 101

Query: 310  358
           
Sbjct: 149  101

[Close]

BLAST of WMU14543 vs. Swiss-Prot
Match: MTPA2_ARATH (Metal tolerance protein A2 OS=Arabidopsis thaliana GN=MTPA2 PE=2 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 7.4e-09
Identity = 32/34 (94.12%), Postives = 31/34 (91.18%), Query Frame = 1


Query: 256 FMSVEVVGGIKANSLAILTDAAHLLSDVAAFAIS 315
           F+ VEVVGGIKANSLAILTDAAHLLSDVAAFAIS
Sbjct: 84  FIVVEVVGGIKANSLAILTDAAHLLSDVAAFAIS 117

Query: 316  358
           
Sbjct: 144  117

[Close]

BLAST of WMU14543 vs. Swiss-Prot
Match: MTPA1_ARATH (Metal tolerance protein A1 OS=Arabidopsis thaliana GN=MTPA1 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 1.8e-07
Identity = 29/34 (85.29%), Postives = 29/34 (85.29%), Query Frame = 1


Query: 256 FMSVEVVGGIKANSLAILTDAAHLLSDVAAFAIS 315
           FMS+EVV GIKANSLAIL DAAHLL+DV AFAIS
Sbjct: 43  FMSIEVVCGIKANSLAILADAAHLLTDVGAFAIS 76

Query: 316  358
           
Sbjct: 103  76

[Close]

BLAST of WMU14543 vs. Swiss-Prot
Match: MTPB_ARATH (Metal tolerance protein B OS=Arabidopsis thaliana GN=MTPB PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 2.6e-06
Identity = 24/33 (72.73%), Postives = 27/33 (81.82%), Query Frame = 1


Query: 259 MSVEVVGGIKANSLAILTDAAHLLSDVAAFAIS 318
           MSV++VGG KANSLA++TDAAHLLSDVA   +S
Sbjct: 69  MSVQIVGGFKANSLAVMTDAAHLLSDVAGLCVS 101

Query: 319  358
           
Sbjct: 129  101

[Close]

BLAST of WMU14543 vs. TrEMBL
Match: A0A0A0KD36_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G080940 PE=4 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 7.6e-13
Identity = 36/38 (94.74%), Postives = 37/38 (97.37%), Query Frame = 2


Query: 56  EMEVQDHGHIIEVCGDVQAVGPSTVGSKICGDAPSCGF 115
           +MEVQDHGHIIEVCGDVQAVGPS VGSKICGDAPSCGF
Sbjct: 7   QMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGF 44

Query: 116  170
           
Sbjct: 67   44

[Close]

BLAST of WMU14543 vs. TrEMBL
Match: A0A0A0KD36_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G080940 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 5.7e-08
Identity = 34/34 (100.00%), Postives = 34/34 (100.00%), Query Frame = 1


Query: 256 FMSVEVVGGIKANSLAILTDAAHLLSDVAAFAIS 315
           FMSVEVVGGIKANSLAILTDAAHLLSDVAAFAIS
Sbjct: 74  FMSVEVVGGIKANSLAILTDAAHLLSDVAAFAIS 107

Query: 316  358
           
Sbjct: 134  107

HSP 2 Score: 80.9 bits (198), Expect = 1.3e-12
Identity = 36/37 (97.30%), Postives = 36/37 (97.30%), Query Frame = 2


Query: 59  MEVQDHGHIIEVCGDVQAVGPSTVGSKICGDAPSCGF 118
           MEVQDHGHIIEVCGDVQAVGPS VGSKICGDAPSCGF
Sbjct: 1   MEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGF 37

Query: 119  170
           
Sbjct: 61   37

[Close]

BLAST of WMU14543 vs. TrEMBL
Match: A7L8D2_CUCSA (Metal tolerance protein OS=Cucumis sativus GN=MTP PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 5.7e-08
Identity = 34/34 (100.00%), Postives = 34/34 (100.00%), Query Frame = 1


Query: 256 FMSVEVVGGIKANSLAILTDAAHLLSDVAAFAIS 315
           FMSVEVVGGIKANSLAILTDAAHLLSDVAAFAIS
Sbjct: 67  FMSVEVVGGIKANSLAILTDAAHLLSDVAAFAIS 100

Query: 316  358
           
Sbjct: 127  100

HSP 2 Score: 80.9 bits (198), Expect = 1.3e-12
Identity = 36/37 (97.30%), Postives = 36/37 (97.30%), Query Frame = 2


Query: 59  MEVQDHGHIIEVCGDVQAVGPSTVGSKICGDAPSCGF 118
           MEVQDHGHIIEVCGDVQAVGPS VGSKICGDAPSCGF
Sbjct: 1   MEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGF 37

Query: 119  170
           
Sbjct: 61   37

[Close]

BLAST of WMU14543 vs. TrEMBL
Match: E5GC92_CUCME (Metal tolerance protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 5.7e-08
Identity = 34/34 (100.00%), Postives = 34/34 (100.00%), Query Frame = 1


Query: 256 FMSVEVVGGIKANSLAILTDAAHLLSDVAAFAIS 315
           FMSVEVVGGIKANSLAILTDAAHLLSDVAAFAIS
Sbjct: 67  FMSVEVVGGIKANSLAILTDAAHLLSDVAAFAIS 100

Query: 316  358
           
Sbjct: 127  100

HSP 2 Score: 70.9 bits (172), Expect = 1.3e-09
Identity = 43/73 (58.90%), Postives = 50/73 (68.49%), Query Frame = 1


Query: 190 KRCKGAICVYAEALHSS-------CSLHR----------FMSVEVVGGIKANSLAILTDA 249
           K CKGA C +++A +SS        S+ +          FM+VEVVGGIKANSLAILTDA
Sbjct: 45  KFCKGAACDFSDASNSSKDARERSASMRKLIVAVVLCVVFMAVEVVGGIKANSLAILTDA 104

Query: 250 AHLLSDVAAFAIS 309
           AHLLSDVAAFAIS
Sbjct: 105 AHLLSDVAAFAIS 117

Query: 310  358
           
Sbjct: 165  117

[Close]

BLAST of WMU14543 vs. TrEMBL
Match: A0A096QDE4_MAIZE (Uncharacterized protein OS=Zea mays GN=umc2311 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.3e-09
Identity = 43/73 (58.90%), Postives = 50/73 (68.49%), Query Frame = 1


Query: 190 KRCKGAICVYAEALHSS-------CSLHR----------FMSVEVVGGIKANSLAILTDA 249
           K CKGA C +++A +SS        S+ +          FM+VEVVGGIKANSLAILTDA
Sbjct: 30  KFCKGAACDFSDASNSSKDARERSASMRKLIVAVVLCVVFMAVEVVGGIKANSLAILTDA 89

Query: 250 AHLLSDVAAFAIS 309
           AHLLSDVAAFAIS
Sbjct: 90  AHLLSDVAAFAIS 102

Query: 310  358
           
Sbjct: 150  102

[Close]

BLAST of WMU14543 vs. NCBI nr
Match: gi|659120153|ref|XP_008460041.1| (PREDICTED: metal tolerance protein 1-like isoform X1 [Cucumis melo])

HSP 1 Score: 82.0 bits (201), Expect = 8.4e-13
Identity = 39/49 (79.59%), Postives = 41/49 (83.67%), Query Frame = 2


Query: 23  LHQLQLSSPPTEMEVQDHGHIIEVCGDVQAVGPSTVGSKICGDAPSCGF 82
           +H  QL     +MEVQDHGHIIEVCGDVQAVGPS VGSKICGDAPSCGF
Sbjct: 1   MHTTQL-----QMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGF 44

Query: 83   142
           
Sbjct: 61   44

Query: 143  170
           
Sbjct: 121  44

[Close]

BLAST of WMU14543 vs. NCBI nr
Match: gi|449454576|ref|XP_004145030.1| (PREDICTED: metal tolerance protein 1-like [Cucumis sativus])

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-12
Identity = 36/38 (94.74%), Postives = 37/38 (97.37%), Query Frame = 2


Query: 56  EMEVQDHGHIIEVCGDVQAVGPSTVGSKICGDAPSCGF 115
           +MEVQDHGHIIEVCGDVQAVGPS VGSKICGDAPSCGF
Sbjct: 7   QMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGF 44

Query: 116  170
           
Sbjct: 67   44

[Close]

BLAST of WMU14543 vs. NCBI nr
Match: gi|821595393|ref|NP_001295856.1| (uncharacterized protein LOC101213602 [Cucumis sativus])

HSP 1 Score: 80.9 bits (198), Expect = 1.9e-12
Identity = 36/37 (97.30%), Postives = 36/37 (97.30%), Query Frame = 2


Query: 59  MEVQDHGHIIEVCGDVQAVGPSTVGSKICGDAPSCGF 118
           MEVQDHGHIIEVCGDVQAVGPS VGSKICGDAPSCGF
Sbjct: 1   MEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGF 37

Query: 119  170
           
Sbjct: 61   37

[Close]

BLAST of WMU14543 vs. NCBI nr
Match: gi|659120155|ref|XP_008460043.1| (PREDICTED: metal tolerance protein 1-like isoform X2 [Cucumis melo])

HSP 1 Score: 80.9 bits (198), Expect = 1.9e-12
Identity = 36/37 (97.30%), Postives = 36/37 (97.30%), Query Frame = 2


Query: 59  MEVQDHGHIIEVCGDVQAVGPSTVGSKICGDAPSCGF 118
           MEVQDHGHIIEVCGDVQAVGPS VGSKICGDAPSCGF
Sbjct: 1   MEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGF 37

Query: 119  170
           
Sbjct: 61   37

[Close]

BLAST of WMU14543 vs. NCBI nr
Match: gi|195619884|gb|ACG31772.1| (metal tolerance protein A2 [Zea mays])

HSP 1 Score: 69.7 bits (169), Expect = 4.3e-09
Identity = 43/73 (58.90%), Postives = 50/73 (68.49%), Query Frame = 1


Query: 190 KRCKGAICVYAEALHSS-------CSLHR----------FMSVEVVGGIKANSLAILTDA 249
           K CKGA C +++A +SS        S+ +          FM+VEVVGGIKANSLAILTDA
Sbjct: 45  KFCKGAACDFSDASNSSKDARERSASMRKLIVAVVLCVVFMAVEVVGGIKANSLAILTDA 104

Query: 250 AHLLSDVAAFAIS 309
           AHLLSDVAAFAIS
Sbjct: 105 AHLLSDVAAFAIS 117

Query: 310  358
           
Sbjct: 165  117

The following BLAST results are available for this feature:

BLAST of WMU14543 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G46800.1	2.2e-11	54.79	zinc transporter of Arabidopsis thaliana	[more]
AT3G58810.1	4.1e-10	94.12	metal tolerance protein A2	[more]
AT3G61940.1	1.0e-08	85.29	Cation efflux family protein	[more]
AT2G29410.1	1.5e-07	72.73	metal tolerance protein B1	[more]

BLAST of WMU14543 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MTP1_ORYSJ	6.0e-11	56.16	Metal tolerance protein 1 OS=Oryza sativa subsp. japonica GN=MTP1 PE=2 SV=1	[more]
MTP1_ARATH	3.9e-10	54.79	Metal tolerance protein 1 OS=Arabidopsis thaliana GN=MTP1 PE=1 SV=2	[more]
MTPA2_ARATH	7.4e-09	94.12	Metal tolerance protein A2 OS=Arabidopsis thaliana GN=MTPA2 PE=2 SV=2	[more]
MTPA1_ARATH	1.8e-07	85.29	Metal tolerance protein A1 OS=Arabidopsis thaliana GN=MTPA1 PE=2 SV=1	[more]
MTPB_ARATH	2.6e-06	72.73	Metal tolerance protein B OS=Arabidopsis thaliana GN=MTPB PE=2 SV=1	[more]

BLAST of WMU14543 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KD36_CUCSA	7.6e-13	94.74	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G080940 PE=4 SV=1	[more]
A0A0A0KD36_CUCSA	5.7e-08	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G080940 PE=4 SV=1	[more]
A7L8D2_CUCSA	5.7e-08	100.00	Metal tolerance protein OS=Cucumis sativus GN=MTP PE=2 SV=1	[more]
E5GC92_CUCME	5.7e-08	100.00	Metal tolerance protein OS=Cucumis melo subsp. melo PE=4 SV=1	[more]
A0A096QDE4_MAIZE	1.3e-09	58.90	Uncharacterized protein OS=Zea mays GN=umc2311 PE=4 SV=1	[more]

BLAST of WMU14543 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659120153\|ref\|XP_008460041.1\|	8.4e-13	79.59	PREDICTED: metal tolerance protein 1-like isoform X1 [Cucumis melo]	[more]
gi\|449454576\|ref\|XP_004145030.1\|	1.1e-12	94.74	PREDICTED: metal tolerance protein 1-like [Cucumis sativus]	[more]
gi\|821595393\|ref\|NP_001295856.1\|	1.9e-12	97.30	uncharacterized protein LOC101213602 [Cucumis sativus]	[more]
gi\|659120155\|ref\|XP_008460043.1\|	1.9e-12	97.30	PREDICTED: metal tolerance protein 1-like isoform X2 [Cucumis melo]	[more]
gi\|195619884\|gb\|ACG31772.1\|	4.3e-09	58.90	metal tolerance protein A2 [Zea mays]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR002524	Cation_efflux
IPR027469	Cation_efflux_TMD_sf

Vocabulary: **Biological Process**
Term	Definition
GO:0006812	cation transport
GO:0055085	transmembrane transport

Vocabulary: **Molecular Function**
Term	Definition
GO:0008324	cation transmembrane transporter activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006812	cation transport
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008324	cation transmembrane transporter activity

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Cla007010	Cla007010	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
S3_0099246	S3_0099246	EST
S3_0107970	S3_0107970	EST
S3_0120677	S3_0120677	EST
S3_0139352	S3_0139352	EST

InterPro

Analysis Name: InterPro Annotations of watermelon unigene v2
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002524	Cation efflux protein	PANTHER	PTHR11562	CATION EFFLUX PROTEIN/ ZINC TRANSPORTER	coord: 35..100 score: 2.9
IPR002524	Cation efflux protein	PFAM	PF01545	Cation_efflux	coord: 58..101 score: 5.
IPR027469	Cation efflux protein transmembrane domain	GENE3D	G3DSA:1.20.1510.10		coord: 55..101 score: 6.5
None	No IPR available	PANTHER	PTHR11562:SF31	RE54080P	coord: 35..100 score: 2.9
None	No IPR available	unknown	SSF161111	Cation efflux protein transmembrane domain-like	coord: 51..101 score: 2.22